CN101803732B - Health-care food with blood-fat reduction assistance function and preparation method thereof - Google Patents
Health-care food with blood-fat reduction assistance function and preparation method thereof Download PDFInfo
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- CN101803732B CN101803732B CN2010101497367A CN201010149736A CN101803732B CN 101803732 B CN101803732 B CN 101803732B CN 2010101497367 A CN2010101497367 A CN 2010101497367A CN 201010149736 A CN201010149736 A CN 201010149736A CN 101803732 B CN101803732 B CN 101803732B
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Abstract
The invention provides a health-care food with a blood-fat reduction assistance function, comprising the following components in percentage by weight: 40 to 60 percent of purple perilla seed oil, 5 to 25 percent of grape seed oil, 2.5 to 22.5 percent of seabuckthorm seed oil, and 12.5 to 32.5 percent of corn oil. Meanwhile, the invention also provides a preparation method for the health-care food, which comprises the following steps: uniformly mixing the purple perilla seed oil, the grape seed oil, the seabuckthorm seed oil and the corn oil in proportion; and filling the mixed materials into capsules. The health-care food has the function of blood-fat reduction assistance without any toxic or side effect, and is applicable to hyperlipidemia people caused by hypercholesterolemia or hypertriglyceridemia.
Description
Technical field
The present invention relates to a kind of health food and preparation method thereof, particularly relate to a kind of health food and preparation method thereof with auxiliary lipid-lowering function.
Background technology
Dyslipidemia is commonly called as high fat of blood, is meant the illness that one or more lipid components increase unusually in the blood, is more common disease.The pathogenic factor of hyperlipidemia; Mainly contain following several respects: (1) Along with people's growth in the living standard; There is quite a few the elderly the part teenager also to be arranged to carbohydrate, lipid material Excessive Intake; Simultaneously lack physical training again, cause lipid material to store up in vivo too much, the obesity that promptly common people say; (2) long-term smoking or great drinker, the people who gets used to sitting quietly, live irregular, mood is emotional, spiritual in tension person, crowds such as postmenopausal women very easily cause the lipid material metabolic disorder, cause that lipid material stores up too much in the blood; (3) diseases such as metabolic disease such as diabetes, hypothyroidism, liver and kidney disease cause the lipid material metabolism bad, cause that lipid material stores up too much in the blood; (4) the medical history person of high fat of blood family is arranged.
High fat of blood still causes hypertensive immediate cause.Vascular lipids material slightly soluble or be insoluble to blood, year in year out, lipid matter can not be transported out blood vessel but inboard attached to blood vessel by blood; The space narrows down in the blood vessel, and therefore under the constant situation of CBF, it is anxious that blood flow intravascular speed becomes; Impulsive force to vascular wall increases; Cause blood pressure higher, along with the continuous increase of lipid matter, blood pressure also further raises.At present, the depressor kind on the market is a lot, and function also alternates betwwen good and bad, but the principle of these depressor is not to remove the vascular lipids material but hemangiectasis, thereby reduces velocity of blood flow, reduces impact, the pressure of blood to vascular wall.Though this medicine can play the effect of step-down within a certain period of time; But along with the increase of vascular lipids material, just need to strengthen dosage, more serious consequence is that blood vessel is by unlimited expansion; Cause the vascular wall attenuation; In case increase dosage once again, cause angiorrhoxis probably, threat to life safety.Therefore, it is very necessary selecting the health food of auxiliary antilipemic.
Summary of the invention
Goal of the invention: the object of the present invention is to provide a kind of health food with auxiliary lipid-lowering function.
Another object of the present invention provides the preparation method of above-mentioned health food.
Technical scheme: a kind of health food with auxiliary lipid-lowering function comprises following components in weight percentage: 40～60% Purple Perilla Seed Oil, 5～25% grape-kernel oil, 2.5～22.5% Seabuckthorm Seed Oil, 12.5～32.5% corn oil.
Wherein, Purple Perilla Seed Oil is by the vacuum outgas of purple perilla seed, adds CO
2, extraction, separate and to form.
Wherein, grape-kernel oil is by the grape pip vacuum outgas, adds CO
2, extraction, separate and to form.
Wherein, Seabuckthorm Seed Oil is by the seabuckthorn seeds vacuum outgas, adds CO
2, extraction, separate and to form.
Prepare described a kind of method, may further comprise the steps with health food of auxiliary lipid-lowering function:
(1) pressing formula ratio mixes Purple Perilla Seed Oil, grape-kernel oil, Seabuckthorm Seed Oil and corn oil;
(2) mixed material is incapsulated.
Wherein, the hybrid mode of step (1) was advisable with intermittent stirring at normal temperatures in 1 hour, made mixed material become uniform liquid.
Beneficial effect: the present invention adopts natural material, has auxiliary lipid-lowering function, scientific formulation, and processing method is simple, does not bring any impurity into, has no side effect, and is fit to the people with hyperlipidemia that high cholesterol or high triglyceride cause.
The specific embodiment
Embodiment 1:
Get 40% Purple Perilla Seed Oil (unit is a percetage by weight, down with), 25% grape-kernel oil, 7.5% Seabuckthorm Seed Oil and 27.5% corn oil, intermittent at normal temperatures the stirring 1 hour incapsulates mixed material every 0.5g then.
Embodiment 2:
Get 45% Purple Perilla Seed Oil, 20% grape-kernel oil, 2.5% Seabuckthorm Seed Oil and 32.5% corn oil, intermittent at normal temperatures the stirring 1 hour incapsulates mixed material every 0.5g then.
Embodiment 3:
Get 50% Purple Perilla Seed Oil, 15% grape-kernel oil, 12.5% Seabuckthorm Seed Oil and 22.5% corn oil, intermittent at normal temperatures the stirring 1 hour incapsulates mixed material every 0.5g then.
Embodiment 4:
Get 55% Purple Perilla Seed Oil, 10% grape-kernel oil, 22.5% Seabuckthorm Seed Oil and 12.5% corn oil, intermittent at normal temperatures the stirring 1 hour incapsulates mixed material every 0.5g then.
Embodiment 5:
Get 60% Purple Perilla Seed Oil, 5% grape-kernel oil, 17.5% Seabuckthorm Seed Oil and 17.5% corn oil, intermittent at normal temperatures the stirring 1 hour incapsulates mixed material every 0.5g then.
Embodiment 6:
The zoopery of auxiliary lipid-lowering function of the present invention
1. material
1.1 sample: provide by the Wuxi City health bio tech ltd of being bestowed by heaven.The oral recommended amounts of human body is every day 2 times, each 2, and become body weight for humans press 60kg calculating, amount to dosage 0.0333g/kg.bw.
1.2 experiment equipment, animal and feed
1.2.1 experiment equipment
OLYMPUS AU400 automatic clinical chemistry analyzer, cholesterol (CHOL), triglycerides (TG) kit, HDL-C (HDL-C) kit.Above equipment is provided by the disease prevention and control center, Hunan Province.
1.2.2 animal used as test
40 of SPF level male SD rats provide animal used as test production licence number SCXK (Henan) 2005-0001 by Henan Province's Experimental Animal Center.Feed is provided by Changsha Kaifu District east wound animal used as test science service portion, and production licence number is SCXK (Hunan) 2006-0001.Experiment condition is a barrier environment, 22～24 ℃ of experimental session experimental situation temperature, humidity 56～58%.The animal used as test occupancy permit number is SYXK (Hunan) 2005-0001.
1.2.3 high lipid food
78.8% basal feed, 1% cholesterol, 10% yolk powder, 10% lard, 0.2% cholate.
2. experiment
2.1 dosage design
If basic, normal, high dosage is respectively 0.167g/kg.bw, 0.333g/kg.bw, 1.000g/kg.bw (be equivalent to respectively human body recommended amounts 5,10,30 times); Being tried the thing preparation time gets content 3.34g of the present invention, 6.66g, 20.00g respectively and adds vegetable oil and be assigned to 100ml; Give animal subject respectively and irritate stomach, once a day, irritate the long-pending 0.5ml/100g.bw of being of body of stomach; Continuous 30 days, control group gave the equal-volume vegetable oil.
2.2 experimental technique
With basal feed feed raise rat and observe a week after; Fasting 16 hours; Get tail blood; Measure serum total cholesterol (TC), triglycerides (TG), HDL-C (HDL-C) with the OLYMPUSAU400 automatic clinical chemistry analyzer, take into account TG according to the TC level animal is divided into 4 groups at random: high fat control group and basic, normal, high three are tried the thing group.Begin from formal test, each treated animal is used high lipid food instead, designs to each group animal subject by 2.1 dosage simultaneously and irritates stomach.Weigh weekly once, finish fasting 16 hours, pull out the eyeball blood sampling and measure serum TC, TG, HDL-C in experiment.
3. data
Data are carried out statistical analysis with Excel2003, Spss11.0 software.Earlier data are carried out homogeneity test of variance,, adopt one-way analysis of variance totally to compare, find differences again and to carry out comparing in twos between a plurality of dose groups and control group mean with the Dunnett method if variance is neat.If heterogeneity of variance, then initial data is carried out suitable variable conversion, satisfy homogeneity test of variance after, add up with the data after the conversion; If do not reach the neat purpose of variance yet after the variable conversion, to use rank test instead and add up, discovery is overall more variant, then adopts Tamhane ' the sT2 check that does not require homogeneity of variance to compare.
4. the result judges
4.1 the auxiliary lipid-lowering function result judges
In serum total cholesterol, triglycerides, HDL-C detect, serum total cholesterol, the triglycerides binomial index positive, this given the test agent auxiliary lipid-lowering function zoopery of decidable result is positive.
4.2 the auxiliary triglycerides result that reduces judges
1. two dose groups results of triglycerides are positive; 2. dose groups result of triglycerides is positive, and HDL-C is significantly higher than control group simultaneously, the auxiliary triglycerides zoopery result positive that reduces of this given the test agent of decidable.
4.3 the auxiliary serum total cholesterol result that reduces judges
1. two dose groups results of serum total cholesterol are positive; 2. dose groups result of serum total cholesterol is positive, and HDL-C is significantly higher than control group simultaneously, the auxiliary serum total cholesterol zoopery result positive that reduces of this given the test agent of decidable.
5. result
5.1 the present invention is to the influence of rat body weight
Table 1 the present invention is to the influence
of rat body weight
Visible by table 1, tried thing treated animal body weight and experimental session body weight gain and high fat control group before and after the experiment relatively, difference does not have conspicuousness (P＞0.05), explains that the present invention does not have influence to rat body weight.
5.2 the present invention is to the influence of Serum TC, TG, HDL-C
Respectively organize Serum TC level
before and after table 2 experiment
Respectively organize rat blood serum TG level
before and after table 3 experiment
Respectively organize rat blood serum DHL-C level
before and after table 4 experiment
Visible by table 2～4, after the experiment, high fat control group serum TC, TG all obviously raise, and relatively preceding with experiment, difference has conspicuousness (P＜0.05), surperficial modeling success.Compare with high fat control group, middle and high dose groups can significantly reduce the rat blood serum triglyceride levels, and high dose group can significantly reduce rat total cholesterol level (P＜0.05).
6. sum up
Under this experiment condition; Content of the present invention with 0.167g/kg.bw, 0.333g/kg.bw, 1.000g/kg.bw dosage is given SD level rat oral gavage 30 days; Compare with high fat control group; 0.333g/kg.bw, 1.000g/kg.bw dosage can significantly reduce and raise high lipid food rat blood serum triglyceride levels, 1.000g/kg.bw dosage can significantly reduce raises high lipid food rat blood serum total cholesterol level (P＜0.05).Judge that according to " health food check and assessment technique standard " (version in 2003) the present invention has auxiliary lipid-lowering function to animal.
Embodiment 7:
The human experiment experiment of auxiliary lipid-lowering function of the present invention
1. material
1.1 sample: provided by the Wuxi City health bio tech ltd of being bestowed by heaven, be divided into capsule No. 1, No. 2, wherein No. 1 capsule is a placebo, and No. 2 capsule is a content of the present invention.The oral RD of human body is every day 2 times, each 2.
1.2 the experimenter selects
1.2.1 the standard of including in
Experimenter's male or female, 18～65 years old age.Blood sampling is 2 times in half a year, 2 serum total cholesterols all >=5.2mmol/L or serum triglyceride >=1.65mmol/L, simple dyslipidemia does not have obvious brain, the heart, liver, lung, kidney, blood illness, does not have the history of taking medicine for a long time, receives examination to guarantee to cooperate voluntarily.
1.2.2 experimenter's exclusion standard
Gestation or women breast-feeding their children are to the health food allergy sufferers; Merge to have the inclination, serious disease patients such as liver, kidney and hemopoietic system; Take the article relevant in a short time, have influence on judgement person as a result with being tried function; Do not meet the standard of including in,, can't judge effect or data not umbra sound effect or security judgement person not by the practical given the test agent of regulation.
2. experiment
2.1 design and grouping
Adopt two kinds of control design between self and group.Be divided into capsule test-meal group of the present invention and placebo group at random by experimenter's blood lipid level, consider influence result's principal element such as age, sex, diet etc. as far as possible, carry out harmony to check, with the comparativity between the assurance group.Carry out the test-meal test by double-blind study.
2.2 experimental technique
The experimenter takes sample by RD every day, takes continuously 45 days.Duration of test does not change original eating habit, normal diet.
3. observation index
Each measures once each item observation index when test-meal on-test and end.
3.1 safety indexes
3.1.1 general physical examination: perquisition and understand experimenter's situation such as spirit, sleep, diet, stool and urine, body weight, blood pressure, changes in heart rate before and after the experiment with measuring before the test.
3.1.2 routine blood test: red blood cell count(RBC), white blood cell count(WBC) and classification, content of hemoglobin mensuration etc.
3.1.3 routine urinalysis: pH value, leucocyte, glucose in urine etc.
3.1.4 stool routine examination.
3.1.5 Abdominal B type ultrasonography, electrocardiogram, X line fluoroscopy of chest inspection (measuring once before on-test).
3.1.6 blood biochemistry index inspection: total serum protein (TP), albumin (ALB), glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease (AST), uric acid (UA), urea nitrogen (BUN), creatinine (Cr), blood sugar mensuration such as (GLU).
3.1.7 bad reaction is observed.
3.2 effect index
Serum total cholesterol (CHOL), triglycerides (TG), HDL-C (HDL-C).
4. the result judges
4.1CHOL reduce＞10%; TG reduces＞15%; HDL-C rising＞0.104mmol/L; Between group statistical significance is arranged more all after each effect observation index test front and back self comparison and the test-meal, can judge that this index is positive.
4.2 serum total cholesterol, the triglycerides binomial index positive; HDL-C significantly is not lower than control group; Decidable is tried thing and is had the auxiliary lipid-lowering function effect: positive in serum total cholesterol, the triglycerides binomial index; HDL-C significantly is not lower than control group, and decidable is tried thing and has auxiliary reduce serum total cholesterol or the effect of auxiliary reduction triglycerides.
5. statistical procedures
The result representes with mean ± standard deviation, and self paired data adopts paired t-test, between test group and the control group under the neat prerequisite of variance; Mean relatively adopts t check in groups, transforms otherwise carry out variable, satisfies the neat back of variance and adopts the t check; If variance is still uneven, adopt rank test.
6. result
6.1 security is observed
6.1.1 ordinary circumstance: test-meal group 53 examples, control group 53 examples.Before and after the test-meal, examination trencherman spirit, sleep, diet, stool and urine situation no abnormality seen; Control group: man/woman is 20/33, and the age is 46.74 ± 11.56 years old; The test-meal group: man/woman is 20/33, and the age is 46.72 ± 11.55.
6.1.2 Abdominal B type ultrasonography, electrocardiogram, the fluoroscopy of chest of X line detect: all in normal range (NR).
6.1.3 body weight, blood pressure, heart rate, routine urinalysis, stool routine examination, routine blood test index situation of change
Body weight, blood pressure, heart rate, routine urinalysis, stool routine examination and routine blood test situation of change
before and after table 5 test-meal
Visible by table 5, test-meal is tried the forward and backward test-meal group of thing and control group body weight, blood pressure, heart rate, routine urinalysis, stool routine examination and routine blood test are not all seen obvious ANOMALOUS VARIATIONS, explains that the present invention does not have the harmful effect of above-mentioned aspect.
6.1.4 blood biochemistry index situation of change before and after the test-meal test
Blood biochemistry index situation of change
before and after the table 6 test-meal test
Visible by table 6; Test-meal is tried the forward and backward test-meal group of thing and control group total serum protein (TP), albumin (ALB), glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease (AST), creatinine (Cr), blood sugar (GLU), uric acid (UA), urea nitrogen (BUN) is and sees obvious ANOMALOUS VARIATIONS, explains that the present invention does not have the harmful effect of above-mentioned aspect.
6.1.5 do not see obvious adverse reaction during the test-meal.
6.2 effect is observed
Change of serum C HOL, TG, HDL-C level
before and after the table 7 test-meal test
Change of serum C HOL, TG, HDL-C level
before and after the table 8 test-meal test
Annotate:
*With comparison P＜0.05 before the test-meal, # and control group be P＜0.05 relatively.
Change of serum C HOL, TG, HDL-C situation of change before and after table 9 test-meal
Visible by table 7～9, blood sampling twice in half a year is respectively test-meal preceding 1 and the preceding 2 blood fat data of test-meal, divides into groups and statistical disposition near 2 blood lipid levels before taking the test-meal that is tried thing with the date.Control group and test-meal group change of serum C HOL, TG level compare before the test, and difference does not have conspicuousness (P＞0.05), points out between two groups to have comparativity.Compare before CHOL, TG and the test-meal and after the control group test-meal after the test-meal of test-meal group, difference all has conspicuousness (P＜0.05).The HDL-C level is obviously to change (P＞0.05) before and after the test-meal.
7. conclusion
Adopt counter point between own control and group; The volunteer that selection meets experimental condition takes and is tried thing after 45 days; The result shows: take the present invention and tried before CHOL after the test-meal of thing test group, TG and the test-meal relatively; Difference has conspicuousness (P＜0.05), and descends 10.21%, 20.99% before CHOL, TG and the test-meal more respectively after the test-meal; Compare before test-meal group CHOL, TG and the test-meal and after the control group test-meal after the test-meal, difference has conspicuousness (P＜0.05); Test-meal group HDL-C and control group comparing difference do not have conspicuousness (P＞0.05) after the test-meal.Do not see obvious adverse reaction during the test-meal.According to " health food check and assessment technique standard " (version in 2003) evaluation criterion, can judge that the present invention has the auxiliary lipid-lowering function effect.
Claims (2)
1. the health food with auxiliary lipid-lowering function is characterized in that it comprises following components in weight percentage: 40～60% Purple Perilla Seed Oil, 5～25% grape-kernel oil, 2.5～22.5% Seabuckthorm Seed Oil, 12.5～32.5% corn oil; Wherein, said Purple Perilla Seed Oil is by the vacuum outgas of purple perilla seed, adds CO
2, extraction, separate and to form, said grape-kernel oil is by the grape pip vacuum outgas, adds CO
2, extraction, separate and to form, said Seabuckthorm Seed Oil is by the seabuckthorn seeds vacuum outgas, adds CO
2, extraction, separate and to form.
2. prepare the described a kind of method of claim 1, it is characterized in that it may further comprise the steps with health food of auxiliary lipid-lowering function:
(1) pressing formula ratio mixes Purple Perilla Seed Oil, grape-kernel oil, Seabuckthorm Seed Oil and corn oil;
(2) mixed material is incapsulated.
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| CN2010101497367A CN101803732B (en) | 2010-04-19 | 2010-04-19 | Health-care food with blood-fat reduction assistance function and preparation method thereof |
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| CN101803732B true CN101803732B (en) | 2012-08-22 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103039984A (en) * | 2012-12-28 | 2013-04-17 | 杨石 | Health-care food for assisting in reducing blood fat |
| CN115414433B (en) * | 2021-10-14 | 2023-08-18 | 广东怡康生物科技有限公司 | Vegetable oil compound soft capsule with health care effect and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1281032A (en) * | 2000-08-21 | 2001-01-24 | 孙传经 | Process for back extraction of medicinal plant oil with supercritical CO2 |
| CN1363363A (en) * | 2001-09-02 | 2002-08-14 | 白山市永利药业股份有限公司 | Grape seed preparation and its extracting process |
| CN101214049A (en) * | 2007-12-26 | 2008-07-09 | 南方李锦记有限公司 | Health food with auxiliary blood fat reducing function |
| CN101366522A (en) * | 2008-10-17 | 2009-02-18 | 罗红宇 | Edible nourishing oil with balanced fatty acid rate |
-
2010
- 2010-04-19 CN CN2010101497367A patent/CN101803732B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1281032A (en) * | 2000-08-21 | 2001-01-24 | 孙传经 | Process for back extraction of medicinal plant oil with supercritical CO2 |
| CN1363363A (en) * | 2001-09-02 | 2002-08-14 | 白山市永利药业股份有限公司 | Grape seed preparation and its extracting process |
| CN101214049A (en) * | 2007-12-26 | 2008-07-09 | 南方李锦记有限公司 | Health food with auxiliary blood fat reducing function |
| CN101366522A (en) * | 2008-10-17 | 2009-02-18 | 罗红宇 | Edible nourishing oil with balanced fatty acid rate |
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