CN106924408A - A kind of Chinese medicine composition for being beneficial to high fat of blood and diabetes B crowd - Google Patents
A kind of Chinese medicine composition for being beneficial to high fat of blood and diabetes B crowd Download PDFInfo
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Abstract
The invention discloses a kind of Chinese medicine composition for being beneficial to high fat of blood and diabetes B crowd, the bulk drug of composition is emblic, cassia seed, hoveniae semoveniae semen, raspberry and the fruit of Chinese magnoliavine.Chinese medicine composition of the invention, both scorching marks of clearing lung-heat stomach and liver, and kidney tonifying deficiency of Yin is originally；Hypoglycemic promotes the production of body fluid to quench thirst and is used in combination with relieving alcoholism, has both taken the excellent of the current internal organs of wine, and except the disadvantage of its wine poison；The five internal organs are simultaneously controlled, five kinds of Chinese medicine, and conscience spleen and lung kidney is incorporated to, Mutiple Targets synthesis treatment, have good health-care effect to high fat of blood, diabetes B crowd.
Description
Technical field
It is more particularly to a kind of to be beneficial in high fat of blood and diabetes B crowd the present invention relates to a kind of Chinese medicine composition
Drug composition.
Background technology
China is onset diabetes number highest country of the world, is found according to research recently, 18 years old and above adult's sample
In, diabetes prevalence is 11.6%, about 1.139 hundred million people.Wherein more than 95% belongs to diabetes B.This kind of diabetic population
Feature is that many, economic condition of middle age morbidity is relatively preferable.Therefore they are also simultaneously simultaneously Drinking rate crowds high.
Diabetes are one group of metabolic syndromes being characterized with hyperglycaemia, can cause various acute and chronic complication.Liver is made
It is the regulation most important organ of blood sugar, effect of the liver function in treating diabetes is now of interest by increasing people.According to
The diabetic of report 50% can merge fatty liver, and severe patient can develop into cirrhosis.And alcohol produces acetaldehyde in vivo, liver
Dirty can be acetic acid discharge by oxidation of acetaldehyde.But if insobriety, beyond the detoxification ability of liver, what is influenceed first is also liver
Dirty, fatty liver is the earliest sign for occurring, and long time integration may result in " fibrosis ", become AML.Hypoglycemic medicinal liquor
Influence to liver in addition to hypoglycemic be also need pay close attention to problem.
Issued first according to many unit consolidations such as Chinese health care association, Chinese Medical Association《Chinese common people's health is drunk shape
Condition survey report》It has been shown that, current China men and women drink ratio respectively up to 84.1% and 29.3%.Many diabetes Bs are suffered from
Person, long-term, excessive consumption of alcohol is unfavorable to glycemic control, but they due to a variety of causes cannot abstinence from alcohol.
The existing wine containing Chinese medicine, partly containing the Chinese medicine for being not suitable as health food, drink has not for a long time to human body
Profit influence, basic effect without hypoglycemic fat-regulating.Additionally, the taste of traditional Chinese medicine of part wine is denser, mouthfeel is not good.
Diabetes and excessive drinking have turned into world's public health problem of serious threat human health, based on China's national situation, I
State diabetic 95% is above is diabetes B patient, it is morbidity above middle age that they are mostly.This kind of crowd is also the height of excessive drinking
Danger crowd.It is effectively one of important measures of control blood sugar and its complication to people's diabetic population above middle age limit wine.Can
While low alcohol consumption, with traditional Chinese medical theory, by adding the medicine with effects such as hypoglycemic, lipid-loweringings in wine, control is assisted
Blood sugar processed, will provide a kind of new treatment thoughts and method for above-mentioned diabetic.
The content of the invention
It is an object of the invention to provide a kind of Chinese medicine composition for being beneficial to high fat of blood and diabetes B crowd.
The technical solution used in the present invention is：
A kind of Chinese medicine composition, the bulk drug of its active component is emblic, cassia seed, hoveniae semoveniae semen, raspberry and the fruit of Chinese magnoliavine.
Used as the further improvement of above-mentioned Chinese medicine composition, the mass ratio of bulk drug is：50~70 parts of emblic, cassia seed
20~30 parts, 10~20 parts of hoveniae semoveniae semen, 10~20 parts of raspberry, 5~10 parts of the fruit of Chinese magnoliavine.
Used as the further improvement of above-mentioned Chinese medicine composition, raw material the effective elements of the medicine is its alcohol extract.
Above-mentioned Chinese medicine composition can be used to prepare the health food for being beneficial to high fat of blood, diabetic population, particularly health care
Wine.
A kind of health liquor, wine is contained within above-mentioned Chinese medicine composition or its extract.
As the further improvement of above-mentioned health liquor, flavouring is also added with wine.
Used as the further improvement of above-mentioned health liquor, flavouring is cloves or its extract.
As the further improvement of above-mentioned health liquor, in the bulk drug of every 100 mass parts, the fourth of 1~3 mass parts is added with
It is fragrant.
A kind of manufacture craft of health liquor, including above-mentioned Chinese medicine composition is immersed in wine, filtered after the completion of immersion
Obtain health liquor.
Used as the further improvement of above-mentioned health liquor manufacture craft, the alcoholic strength of wine is not less than 50v/v%, further,
The alcoholic strength of wine is in 55v/v%~70v/v%.
As the further improvement of above-mentioned health liquor manufacture craft, 50~70 grams, cassia seed 20~30 are added in every liter of wine
Gram, 10~20 grams of hoveniae semoveniae semen, 10~20 grams of raspberry, 5~10 grams of the fruit of Chinese magnoliavine soaked.
The beneficial effects of the invention are as follows：
Chinese medicine composition of the invention, one is to treat both principal and secondary aspect of disease, and both scorching marks of clearing lung-heat stomach and liver, and kidney tonifying deficiency of Yin is originally；Two is drop
Sugar promotes the production of body fluid to quench thirst and is used in combination with relieving alcoholism, has both taken the excellent of the current internal organs of wine, and except the disadvantage of its wine poison；Three are the five internal organs and control, five kinds of Chinese medicine,
Conscience spleen and lung kidney is incorporated to, Mutiple Targets synthesis treatment, has good health-care effect to high fat of blood, diabetes B crowd.
Inventor uses and injects alloxan 200mg/kg to mouse web portion, prepares diabetes model.By 80 modelings into
The mouse of work(be randomly divided into Normal group, model group, five sons promote the production of body fluid hypoglycemic wine (Chinese medicine composition of the present invention), emblic subgroup,
Cassia subgroup, hoveniae semoveniae semen group, raspberry group, five tastes subgroup, gavage five sons and promote the production of body fluid hypoglycemic wine list to diabetic mice respectively
Taste medicine and compatibility extract 0.5g/kg.Determine mouse blood sugar and serum insulin level, observation experiment Mouse Weight, food ration
With amount of drinking water and ordinary circumstance.As a result：Compared with model group, five sons hypoglycemic wine of promoting the production of body fluid can improve experiment mice body weight, food ration
And amount of drinking water, there were significant differences between group；Compared with model group, five sons hypoglycemic wine of promoting the production of body fluid can improve diabetic mice serum insulin
There were significant differences between level, reduction blood sugar, improvement diabetic symptom, group；Compared with model group, emblic subgroup, Cassia subgroup, trifoliate orange
Dulcis subgroups, raspberry group, five groups of five tastes subgroup are to experiment mice body weight, food ration and amount of drinking water, serum insulin level, empty stomach
Blood sugar changes certain improvement result, but without significant difference between group.Conclusion：Five sons hypoglycemic wine of promoting the production of body fluid has that to be obviously improved diabetes small
The general symptom of mouse and hypoglycemic effect, effect are better than exclusive use emblic, cassia seed, hoveniae semoveniae semen, raspberry, in reflection
The compatibility advantage of hospital's agent.
Inventor replicates diabetes B mould using high fat diet joint intraperitoneal injection Streptozotocin (STZ) 30mg/kg
Type.60 successful SD rats of modeling are randomly divided into model group, five sons to promote the production of body fluid hypoglycemic wine (Chinese medicine composition of the present invention) (Wz)
High, medium and low dosage group, common white spirit group, Normal group.Gavage gives relative medicine, normal group and mould respectively for each treatment group
Type group gives physiological saline, totally 8 weeks.A fasting blood-glucose (FBG) is respectively determined at 1,4,8 weekends；8 weekends determined serum glycerine three
Ester (TG), T-CHOL (TC), low-density lipoprotein (LDL-C), HDL (HDL-C) and FPI
(FINS) insulin sensitivity index (ISI), is calculated；Experiment terminates execution rat and takes hepatic tissue detection glutathione peroxidase
(GSH-Px), superoxide dismutase (SOD) activity and MDA (MDA) level.Result compared with Normal group, model group
Rat FBG is significantly raised, (P ＜ 0.01) variant between group；SOD, GSH-Px activity are dramatically increased, and MDA levels are remarkably decreased
Compare, promote the production of body fluid hypoglycemic wine (Wz) height, middle dose group FBG, TG, TC, LDL-C, FINS level of five sons is substantially reduced, HDL-C liters
It is high；Diabetes rat hepatic tissue oxidative stress increases, middle and high dosage group diabetes.Of conclusion five promotes the production of body fluid hypoglycemic wine (Wz)
Blood glucose in diabetic rats, blood fat can be reduced, mitigates insulin resistance.
Specific embodiment
A kind of Chinese medicine composition, the bulk drug of its active component is emblic, cassia seed, hoveniae semoveniae semen, raspberry and the five tastes
Son.
Used as the further improvement of above-mentioned Chinese medicine composition, the mass ratio of bulk drug is：50~70 parts of emblic, cassia seed
20~30 parts, 10~20 parts of hoveniae semoveniae semen, 10~20 parts of raspberry, 5~10 parts of the fruit of Chinese magnoliavine.
Used as the further improvement of above-mentioned Chinese medicine composition, raw material the effective elements of the medicine is its alcohol extract.
A kind of health liquor, wine is contained within above-mentioned Chinese medicine composition or its extract.
As the further improvement of above-mentioned health liquor, flavouring is also added with wine.
Used as the further improvement of above-mentioned health liquor, flavouring is cloves or its extract.
As the further improvement of above-mentioned health liquor, in the bulk drug of every 100 mass parts, the fourth of 1~3 mass parts is added with
It is fragrant.
Cloves can be effectively improved the mouthfeel of wine, reduce the peculiar taste of medicinal material, be easier to wine acceptable.
A kind of manufacture craft of health liquor, including by above-mentioned Chinese medicine composition, be preferably added flavouring and be immersed in wine
In, health liquor is obtained after the completion of immersion.
Related, this area skills such as the soak time of medicinal material and the alcoholic strength of wine, environment temperature, the broken situation of herb in granule form
Art personnel can be adjusted correspondingly according to actual conditions.In general, under 15~25 DEG C of environment temperature, soak time is not
Less than 50 days, to make active ingredient more sufficiently discharge, soak time was more particularly suitable more than 80 days.The alcoholic strength of wine is high,
Soak time can suitably be shortened.
In order to obtain the wine liquid more clarified, physical filtering treatment can be carried out to wine liquid after the completion of immersion.
Used as the further improvement of above-mentioned health liquor manufacture craft, the alcoholic strength of wine is not less than 50v/v%, further,
The alcoholic strength of wine is in 55v/v%~70v/v%.
As the further improvement of above-mentioned health liquor manufacture craft, 50~70 grams, cassia seed 20~30 are added in every liter of wine
Gram, 10~20 grams of hoveniae semoveniae semen, 10~20 grams of raspberry, 5~10 grams of the fruit of Chinese magnoliavine soaked.
As the further improvement of above-mentioned health liquor manufacture craft, flavouring, such as cloves are added during immersion, to adjust health care
The mouthfeel of wine.
Certainly, above-mentioned health liquor is after completing, it is also possible to carry out the modulation of a step, reduces its alcoholic strength, meets
The need for different crowd.
As the further improvement of above-mentioned health liquor manufacture craft, the cloves conduct of 1~3g is added during immersion in every liter of wine
Flavouring.
Fang Xie：Diabetes spp traditional Chinese medical science diabete category, the diabetic for drinking mostly a middle-aged person, its Pathogenesis of Diabetes is generally
It is mark that lung stomach and liver is scorching, and kidney-yin deficiency is this.The total five tastes medicine composition of we, emblic in side is sweet, cool, attaches to the lung and stomach meridians.Tool
Clearing lung-heat stomach is scorching, be monarch drug in a prescription the effect of promote the production of body fluid.Hoveniae semoveniae semen, mild-natured, thoughts of returning home spleen and lung warp, with only wine poison, relieving restlessness of quenching the thirst effect,
It is ministerial drug, one side principal drug assistance is scorching clearly, promotes the production of body fluid to quench thirst, another aspect relieving alcoholism, reduction is drunk and causes discomfort.Cassia seed, bitter,
It is salty, it is slightly cold, Return liver, large intestine channel.With heat-clearing improving eyesight, relax bowel.Be adjutant, one side removing heat from the liver, another aspect defaecation with
Sharp heat toxin is excluded.Heat-clearing takes stopgap measures simultaneously, need to take into account the deficiency of Yin originally.Raspberry, Return liver, kidney, bladder warp.Supplementing the kidney to control the nocturnal contracting urine, supports
Liver improving eyesight.The fruit of Chinese magnoliavine, return lung, the heart, kidney channel.Astringent or styptic treatment for spontaneous sweating, nourishing generate fluid is restrained, kidney calming is adjutant, and one side principal drug assistance promotes the production of body fluid
Quench the thirst, another aspect kidney tonifying enriching yin.All medicine compatibilities, clearing lung-heat stomach and liver of gathering altogether is scorching, the effect that kidney-nourishing promotes the production of body fluid to quench thirst.
With reference to embodiment, technical scheme is further illustrated.
For the sake of aspect, the wine that immersion medicinal material is used in following examples is 60v/v% jowar white wine.It is of course also possible to make
Soaked in the edible distillate spirit of more than 50v/v% with other alcoholic strengthes.
Emblic, raspberry, cassia seed, the fruit of Chinese magnoliavine used in following examples, hoveniae semoveniae semen, cloves are purchased from Guangzhou
The first affiliated hospital of medical pharmaceutical university pharmacy, identifies through Chinese traditional medicine identification teaching and research room professor Zhang Danyan.
Embodiment 1
1000ml alcoholic strengthes are taken for 60v/v% jowar white wine, 60 grams of emblic, 25 grams of cassia seed is added, 15 grams of hoveniae semoveniae semen, is covered basin
Sub 15 grams, 7.5 grams of the fruit of Chinese magnoliavine, in being soaked 100 days in 18 DEG C~22 DEG C constant temperature wine cellars, (five sons promote the production of body fluid hypoglycemic to be filtrated to get medicinal liquor
Wine).
Embodiment 2
1000ml alcoholic strengthes are taken for 60v/v% jowar white wine, 50 grams of emblic, 30 grams of cassia seed is added, 15 grams of hoveniae semoveniae semen, is covered basin
10 grams of son, 10 grams of the fruit of Chinese magnoliavine, 1 gram of cloves, in being soaked 80 days in 18 DEG C~22 DEG C wine cellars, are filtrated to get medicinal liquor.
Embodiment 3
A kind of Chinese medicine composition, by the mass parts of emblic 70, the mass parts of cassia seed 25, the mass parts of hoveniae semoveniae semen 10, the matter of raspberry 20
Amount part, the mass parts of the fruit of Chinese magnoliavine 5, the mass parts of cloves 2 composition.
Embodiment 4
A kind of Chinese medicine composition, by the mass parts of emblic 60, the mass parts of cassia seed 20, the mass parts of hoveniae semoveniae semen 20, the matter of raspberry 10
Amount part, the mass parts of the fruit of Chinese magnoliavine 8, the mass parts of cloves 3 composition.
With reference to experiment, technical scheme is further illustrated
Five sons promote the production of body fluid the influence of hypoglycemic wine simple and compatibility to blood glucose in diabetic mice
1 material and instrument
1.1 animal Kunming mouses, male, body weight (22.1 ± 1.5) g.Purchased from Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center, close
Lattice card numbering:0086745, credit number:SCXK (Guangdong) 2008-0002.Preposition animal is tested in indoor adaptation environment 1 week, room
Temperature control system is fed, standard feed at 20~25 DEG C using posture point cage, and it is running water to drink water.Fasting 12h before modeling, can't help water.
1.2 medicines and reagent
Alloxan lot number:(S8060 Sigma Co., USAs)；Blood sugar test paper (German Roche Diagnostics GmbH, lot number:
2307432)；Glucose estimation kit (Nanjing bioengineering Co., Ltd)；125I- insulin radioimmunoassays medicine box by
U.S. biology Beijing Kemei Biological Technology Co., Ltd. of Beijing section provides (lot number:20110425).
The preparation of 1.3 diabetes animal models is by normal mouse intraperitoneal injection alloxan 200mg/kg, tail vein after 72h
Blood sugar is surveyed in blood sampling, chooses blood glucose value in more than 11.0mmol/L persons.The difference of each group animal average blood sugar value is not more than during packet
0.5mmol/L。
1.4 5 sons promote the production of body fluid hypoglycemic wine：Promoted the production of body fluid hypoglycemic wine using five sons of embodiment 1.
80 diabetic mices that 1.5 packets will be successfully prepared with administration are divided into 8 groups, every group 10, each group by blood glucose value
Blood sugar level is close, and the difference of average must not exceed 0.9mmol/L.8 groups are respectively：Normal group, model group, emblic subgroup
(YGZ), Cassia subgroup (JMZ), hoveniae semoveniae semen group (ZJZ), raspberry group (FBZ), five tastes subgroup (WWZ).Above each group is normal right
Physiological saline is given according to group and model group, five sons promote the production of body fluid, and often 10g body weight gives 0.1mL medicinal liquor amounts to hypoglycemic group (WZSJ), and other are each
Group presses 0.5g/kg extract gastric infusions, 1 time/d, successive administration 15 days respectively.In 1h measuring blood sugar of blood extracting after last dose and
Insulin level, determines body weight before and after experiment.
1.6 observation index
1.6.1 ordinary circumstance activity, diet, drinking-water, body weight of experiment mice etc..The food ration and amount of drinking water of mouse use metal
Metabolic cage is determined.Mouse is respectively placed in metabolic cage before determining, the food ration and amount of drinking water to mouse 24h are remembered respectively
Record.
1.6.2 the measure 8~10h of fasting of fasting blood-glucose (FBS) and serum insulin (FINS), after etherization, from rear
Orbital venous plexus are taken a blood sample.Blood sugar and insulin press the detection of kit operational manual.
Difference between the mean ± standard deviation (x ± s) of 1.7 statistical method each groups, from statistical package treatment.Group
Between difference analysis using t check and one-way analysis of variance.
2 results
The observation of 2.1 general status compared with Normal group, model group mouse on the feed, drinking-water, the aspect of urine volume have increasing
Plus, i.e. the symptom of " three-many-one-little ".After successive administration after 15 days, model group mouse ordinary circumstance is poor, and fur is thick random and unglazed
Pool, muscle power decay, drinking-water, food ration increase more, there were significant differences between group (P<0.01).Compared with model, five sons promote the production of body fluid drop
Sugared group of (WZSJ) mouse spirit is gradually taken a turn for the better, and fur gradually recovers gloss, and drinking-water, dietary amount are gradually reduced, and body weight has increase, group
Between there were significant differences (P<0.05).Compared with model group, emblic subgroup (YGZ), Cassia subgroup (JMZ), hoveniae semoveniae semen group (ZJZ),
Raspberry group (FBZ), five tastes subgroup (WWZ) mouse drinking-water, food ration are reduced, and body weight increased, but without significance difference between group
Different (P>0.05).The results are shown in Table 1.
The each group Mouse Weight of table 1, drinking-water, the change (x ± s) of dietary amount
1. compared with Normal group, P<0.01；2. with model group ratio, P>0.05；3. with model group ratio, P<0.05.
2.2 each experimental group fasting blood-glucoses, serum insulin compare compared with Normal group, and model group mouse blood sugar has bright
It is aobvious to raise, there were significant differences between group (P<0.01)；Serum insulin is decreased obviously, there were significant differences between group (P<0.01).With mould
Type group is compared, and five sons promote the production of body fluid the decline of hypoglycemic group (WZSJ) mouse fasting blood-glucose, there were significant differences between group (P<0.01)；Serum islet
Have and be decreased obviously, there were significant differences between group (P<0.01).Compared with model group, emblic subgroup (YGZ), Cassia subgroup (JMZ),
Hoveniae semoveniae semen group (ZJZ), raspberry group (FBZ), five tastes subgroup (WWZ) mouse fasting blood-glucose has between decline, but group without significance difference
Different (P>0.05)；Serum insulin has between rising, but group without significant difference (P>0.05).The results are shown in Table 2.
The each group mouse fasting blood-glucose of table 2, the change (x ± s) of serum insulin amount
1. compared with Normal group, P<0.01；2. with model group ratio, P>0.05；3. with model group ratio, P<0.05.
3 discuss
This experiment uses model induced by alloxan diabetes animal model, and animal 72h blood sugar almost all after film is made exceedes
14.0mmol/L, and the diabetic symptom of " three high and one low " is presented, hints model is replicated successfully.This result of study shows that five is sub
Hypoglycemic wine of promoting the production of body fluid can obviously reduce model mice fasting blood-glucose (P<0.05) with serum insulin level (P<0.05) glycosuria, is improved
The food ration and amount of drinking water of sick mouse, weight increase；And five sons promote the production of body fluid, each single medicinal liquor of hypoglycemic wine has certain blood sugar decreasing effect,
But compared with model group, not statistically significant (P>0.05).This result of study shows that five sons hypoglycemic wine of promoting the production of body fluid has preferable hypoglycemic
Effect and improvement diabetic symptom effect.Existing grinding shows that five sons promote the production of body fluid each simple emblic of hypoglycemic wine, cassia seed, trifoliate orange Dulcis
Son, raspberry, the extract of the fruit of Chinese magnoliavine have compared with blood sugar reducing function, but its prepare medicinal liquor and not without obvious blood sugar reducing function,
Its mechanism need to be inquired into further.Five sons promote the production of body fluid hypoglycemic wine by emblic, cassia seed, hoveniae semoveniae semen, raspberry, the fruit of Chinese magnoliavine by TCM Recipe
Agent principles of formulating prescriptions compatibility is prepared, diabetes spp traditional Chinese medical science diabete category, the diabetic for drinking mostly a middle-aged person, its diabete
It is mark that machine is generally lung stomach and liver scorching, and kidney-yin deficiency is this.Emblic in side, it is sweet, it is cool, attach to the lung and stomach meridians.Tool clearing lung-heat stomach it is scorching, promote the production of body fluid
The effect of, it is monarch drug in a prescription.Hoveniae semoveniae semen, mild-natured, thoughts of returning home spleen and lung warp, with only wine poison, relieving restlessness of quenching the thirst effect, is ministerial drug, is on the one hand helped
Monarch drug in a prescription is scorching clearly, promotes the production of body fluid to quench thirst, another aspect relieving alcoholism, and reduction is drunk and causes discomfort.Cassia seed, it is bitter, salty, it is slightly cold, it is Return liver, big
Intestines are passed through.With heat-clearing improving eyesight, relax bowel.It is adjutant, one side removing heat from the liver, another aspect defaecation is excluded with sharp heat toxin.Heat-clearing
Take stopgap measures simultaneously, the deficiency of Yin need to be taken into account originally.Raspberry, Return liver, kidney, bladder warp.Supplementing the kidney to control the nocturnal contracting urine, nourishes the liver to improve visual acuity.The fruit of Chinese magnoliavine, returns
Lung, the heart, kidney channel.Astringent or styptic treatment for spontaneous sweating, nourishing generate fluid is restrained, kidney calming is adjutant, and one side principal drug assistance promotes the production of body fluid to quench thirst, on the other hand mended
Kidney enriching yin.All medicine compatibilities, clearing lung-heat stomach and liver of gathering altogether is scorching, the effect that kidney-nourishing promotes the production of body fluid to quench thirst.Our compatible experiments：One is to treat both principal and secondary aspect of disease,
Both the scorching mark of clearing lung-heat stomach and liver, and the kidney tonifying deficiency of Yin is originally；Two is that hypoglycemic promotes the production of body fluid to quench thirst and is used in combination with relieving alcoholism, has both taken the current internal organs of wine
It is excellent, and except its wine poison it；Three are the five internal organs and control, five kinds of Chinese medicine, and conscience spleen and lung kidney is incorporated to.Five sons promote the production of body fluid hypoglycemic wine and single medicine phases
Than there is preferable blood sugar reducing function, compatibility of medicines in a prescription advantage place is further illustrated.
Five sons promote the production of body fluid hypoglycemic wine hypoglycemic and the influence to liver
1 materials and methods
1.1 Experimental Animals Male SD rats, SPF grades, weight (200 ± 2) g, purchased from Traditional Chinese Medicine University Of Guangzhou experimental animal
The heart, quality certification numbering:0086745, credit number:SCXK (Guangdong) 2008-0002.The feeding environment of rat is steady in experimentation
It is fixed, keep room temperature in (25 ± 2) DEG C, relative humidity 50%~70%, feeding environment meets experimental animal environmental facility requirement.
1.2 medicines and reagent：Streptozotocin (STZ) ((lot number:S8060 Sigma Co., USAs)；125I- insulin is put
Penetrate immunoassays medicine box and provide (lot number by U.S. biology Beijing Kemei Biological Technology Co., Ltd. of Beijing section:20110425)；Total courage
Sterol (TC) determines kit (lot number:101091,201011), triglycerides (TG) determines kit (lot number:
104091.201008), HDL-C (HDL-C) determines kit (lot number:100241.201011), low-density
Lipoprotein cholesterol (LDL-C) determines kit (lot number:100271.201011)；Glutathione peroxidase (GSH-Px),
Superoxide Dismutase (SOD) activity and MDA (MDA) determine kit (Bioengineering Research Institute's offer is built up in Nanjing).
1.3 key instrument blood sugar test papers are provided by MediSence Optium companies；BHJ2 blood glucose meters are by MediSence
Optium companies provide；RT2100C enzyme micro-plate readers are provided by Rayto Life and Analytical Sciences Co., Ltd.；SN695B types are put
Immune γ measuring instruments are penetrated by the offer of one factory of day ring instrument of Shanghai atomic nucleus institute；FA (N)/JA (N) electronic balances are by Shanghai Min Qiao essences
Close scientific instrument Co., Ltd provides；37 DEG C of YZB insulating boxs are provided by Guangdong Medical Apparatus and Instruments Factory.UV -2100 type is ultraviolet-and can
See light spectrophotometer (Japanese Shimadzu)；Electro-heating standing-temperature cultivator (Shanghai Yi Heng laboratory apparatus Co., Ltd DHP -9272 type)；
Supercentrifuge (table-type high-speed refrigerated centrifuge:Abbott Laboratories of the U.S.).
1.4 5 sons promote the production of body fluid hypoglycemic wine：Five sons that embodiment 1 is prepared promote the production of body fluid hypoglycemic wine.
After 100 SD rats adaptability of preparation and treatment of 1.5 diabetes rat models are fed 1 week, therefrom randomly select
10 are only used as Normal group, and remaining is used as Glycemia Decline animal.Rats in normal control group standard rat chow feeds (carbon water
Compound accounts for 41.47%, fat and accounts for 14.42%, protein and accounts for 21.06%), and Glycemia Decline group high lipid food feeds (10%
Lard, 2.0% cholesterol, 0.5% cholate, 87.5% basal feed).Disposable intraperitoneal injection STZ 30mg/kg are lured after 4 weeks
Hair hyperglycaemia, tail vein takes blood after 5d, detects blood sugar, chooses the rat of blood sugar ＞ 11.1mmol/L as diabetes model.Just
Normal control group only injects isometric citric acid-sodium citrate buffer solution.Take modeling success rat 60, by blood glucose value, quality with
Machine be divided into model group, five sons promote the production of body fluid the high, medium and low dosage group of hypoglycemic wine (0.5,1.0,2.0g bulk drugs/kg, medicinal liquor 5mL/kg) and
Common white spirit positive controls (5mL/kg), separately set Normal group.Gastric infusion, Normal group and model group are gavaged respectively
Physiological saline, successive administration 8 weeks.During experiment, model group continues to give high fat diet with each experimental group, and Normal group gives
Full diet, each group animal ad lib, drinking-water.
The collection of 1.6 samples and index determining separate serum respectively at the 0th, 4,8 weekend empty stomach tail vein bloods are tested,
For surveying fasting blood-glucose (FBG)；In experiment terminate eve rat prohibit 12h, 10% chloraldurate 3mL/kg anesthesia after abdomen actively
Arteries and veins is put to death after taking blood, leaves and takes serum, for surveying triglycerides (TG), T-CHOL (TC), low-density lipoprotein (LDL-C), height
Density lipoprotein (HDL-C) and serum insulin (FINS) simultaneously calculate insulin sensitivity index (ISI)；Experiment terminates to put to death dynamic
Thing, opens abdominal cavity immediately, takes out liver, is placed in -70 DEG C of ultra low temperature freezers in case surveying hepatic tissue GSH-Px, SOD, MDA.
1.6.1 FBG blood sugar tests are detected using Roche blood-sugar detecting instrument.
1.6.2 lipid determination automatic clinical chemistry analyzer is determined.
1.6.3 insulin (FINS) detection is operated by enzyme linked immunosorbent assay detection kit specification.ISI=ln [1/
(FINS×FBG)]
1.6.4 hepatic tissue GSH-Px, SOD activity, the detection of MDA levels prepares 10% liver tissue homogenate's liquid, refrigerated centrifuge
3000r/min is centrifuged 15min, takes supernatant, is operated in strict accordance with kit explanation step.
The 1.8 statistical procedures softwares of SPSS 11.0, all data are represented with x ± s, and single factor test variance is carried out to data
Analysis (one-way ANOVA), P ＜ 0.05 are that difference is statistically significant.
2 results
2.1 pairs of influences of diabetic model rats fasting blood-glucose (FBG) are compared with Normal group, and model group FBG is significantly raised,
(P ＜ 0.01) variant between group；Compared with model group, after taking 1 week, blood sugar has rising to common white spirit group, but without significance difference
Different (P ＞ 0.05), at the 4th week and the 8th week, blood glucose rise, there were significant differences (P ＜ 0.05) between group；Compared with model group, five sons
Hypoglycemic wine of promoting the production of body fluid each group rat 1 week, 4 weeks, 8 weeks each time point FBG have reduction compared with model group, wherein middle and high dosage group is the 4th
With the 8th week after reduce there were significant differences (P ＜ 0.05), low dose group is without significant difference (P ＞ 0.05).It is shown in Table 3.
Of table 3 five promotes the production of body fluid influence (x ± s) of the hypoglycemic wine to diabetes rat fasting blood-glucose (FBG)
1. compared with Normal group, P<0.01；2. with model group ratio, P>0.05；3. with model group ratio, P<0.05.
Compared with Normal group, model group TG, TC, LDL-C raise (P for 2.2 pairs of influences of fro diabetic rat fat
＜ 0.01), HDL-C declines (P ＜ 0.01), and there were significant differences between group.After being administered 8 weeks, compared with model group, common white spirit group
TG, TC, LDL-C are raised (P ＜ 0.05), and HDL-C declines (P ＜ 0.05), and there were significant differences between group；Compared with model group, five
Son promotes the production of body fluid middle and high dosage group blood fat TG, TC, LDL-C of hypoglycemic wine low (P ＜ 0.05), and HDL-C rises between (P ＜ 0.05), group
There were significant differences；Compared with model group, low dose group, TG, TC, LDL-C raise (P>0.05), HDL-C declines (P>0.05),
But without significant difference between group.The results are shown in Table 4.
Of table 4 five promotes the production of body fluid influence (x ± s) of the hypoglycemic wine to diabetes rat blood fat
1. compared with Normal group, P<0.01；2. with model group ratio, P<0.05；3. with model group ratio, P>0.05.
The influence of 2.3 pairs of Serum of Diabetes Rats insulin (FINS) and Insulin Sensitivity Index (ISI) with just
Normal control group is compared, and model group serum insulin level is raised, and insulin sensitivity index declines, there were significant differences between group (P ＜
0.01)；After being administered 8 weeks, compared with model group, five sons promote the production of body fluid the middle and high dosage group of hypoglycemic wine insulin level decline (P ＜
0.05), ISI increases (P ＜ 0.05), and there were significant differences between group；Low dose group insulin level declines, and insulin sensitivity index increases
Plus, without significant difference (P ＞ 0.05) between group；Common white spirit group insulin level raises (P ＞ 0.05), and ISI reduces (P ＞
0.05), without significant difference between group.The results are shown in Table 5.
Influence (x ± s) of the table 5 to serum insulin (FINS) and Insulin Sensitivity Index (ISI)
1. compared with Normal group, P<0.01；2. with model group ratio, P>0.05；3. with model group ratio, P<0.05.
The influence of 2.4 couples of diabetic model rats livers GSH-Px, SOD, MDA is compared with Normal group, and model group is big
GSH-Px and SOD declines (P ＜ 0.01) in mouse liver, and MDA raises (P ＜ 0.01), and there were significant differences between group, illustrates experimental sugar
Oxidative damage is there may be in the sick rat liver of urine；Compare with model group, five sons promote the production of body fluid that hypoglycemic wine is high, in middle dose group liver
GSH-Px and SOD is raised, and MDA declines, and there were significant differences (P ＜ 0.05) between group, and low dose group is without significant difference (P ＞ 0.05)；
With model group ratio, common white spirit group GSH-Px and SOD decline, and MDA is raised, and there were significant differences (P ＜ 0.05) between group.The results are shown in Table
6。
Influence (x ± s) of the table 6 to diabetes rat liver GSH-Px, SOD, MDA
1. compared with Normal group, P<0.01；2. with model group ratio, P<0.05；3. with model group ratio, P>0.05.
Diabetes B is often accompanied by glucose -lipid metabolism disorder, this test result indicate that, the rats by intraperitoneal injection STZ of fat feeding high
After replicating successfully diabetes B model, there is glucose -lipid metabolism disorder in rat.Give five sons promote the production of body fluid hypoglycemic wine treatment after, except rat
Blood sugar be effectively controlled outer, its disorders of lipid metabolism has obtained a certain degree of improvement, shows that five sons promote the production of body fluid hypoglycemic wine in drop
While hypoglycemia, the blood lipid metabolism of diabetes rat can be adjusted.Oxidative stress is that diabetes trigger the common of each complication
Pathogenesis, is also one of the reason for causing liver fibrosis or even cirrhosis.This research show, diabetes rat liver SOD and
GSH levels decline, and MDA levels are improved, and point out the oxidative damage existed in diabetes rat liver.Five sons hypoglycemic wine of promoting the production of body fluid is controlled
After treatment, diabetes rat liver SOD and GSH levels are raised, and the reduction of MDA levels illustrates that it has certain antioxidation.This
Result of study shows, five sons hypoglycemic wine of promoting the production of body fluid has preferable hypoglycemic, blood fat, mitigates the effect of insulin resistance, to diabetes
The hepar damnification for causing has certain protective effect.
Claims (10)
1. a kind of Chinese medicine composition, the bulk drug of its active component is emblic, cassia seed, hoveniae semoveniae semen, raspberry and the fruit of Chinese magnoliavine.
2. Chinese medicine composition according to claim 1, it is characterised in that：The mass ratio of bulk drug is：Emblic 50~70
Part, 20~30 parts of cassia seed, 10~20 parts of hoveniae semoveniae semen, 10~20 parts of raspberry, 5~10 parts of the fruit of Chinese magnoliavine.
3. Chinese medicine composition according to claim 1, it is characterised in that：Raw material the effective elements of the medicine is its alcohol extract.
4. a kind of health liquor, it is characterised in that：Wine is contained within Chinese medicine composition or its extract described in claim 1 or 2.
5. health liquor according to claim 4, it is characterised in that：Flavouring is also added with wine.
6. health liquor according to claim 5, it is characterised in that：Flavouring is cloves or its extract.
7. health liquor according to claim 5, it is characterised in that：In the bulk drug of every 100 mass parts, 1~3 matter is added with
Measure the cloves of part.
8. a kind of manufacture craft of health liquor, including the Chinese medicine composition described in claim 1 or 2 is immersed in wine, soak
After the completion of obtain health liquor.
9. manufacture craft according to claim 8, it is characterised in that：The alcoholic strength of wine is not less than 50v/v%.
10. manufacture craft according to claim 8 or claim 9, it is characterised in that：50~70 grams, cassia seed are added in every liter of wine
20~30 grams, 10~20 grams of hoveniae semoveniae semen, 10~20 grams of raspberry, 5~10 grams of the fruit of Chinese magnoliavine soaked.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114514912A (en) * | 2022-03-10 | 2022-05-20 | 北京中医药大学第三附属医院 | Construction method of LPS-induced chronic lung injury stagnant heat internal accumulation syndrome mouse model and application of model |
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| CN101002869A (en) * | 2006-12-08 | 2007-07-25 | 深圳微芯生物科技有限责任公司 | Compounding preparation for lowering fat and protecting liver, its preparing method and use |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101002869A (en) * | 2006-12-08 | 2007-07-25 | 深圳微芯生物科技有限责任公司 | Compounding preparation for lowering fat and protecting liver, its preparing method and use |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114514912A (en) * | 2022-03-10 | 2022-05-20 | 北京中医药大学第三附属医院 | Construction method of LPS-induced chronic lung injury stagnant heat internal accumulation syndrome mouse model and application of model |
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