CN101795697A

CN101795697A - Gastropod biological fluid, preparation and process for purification and purposes

Info

Publication number: CN101795697A
Application number: CN200880104094A
Authority: CN
Inventors: 威廉·王; 伊俊; 柯盛; 玛丽亚·哈尔梅拉
Original assignee: PHARMACOM INTERNAT Inc
Current assignee: PHARMACOM INTERNAT Inc
Priority date: 2007-06-23
Filing date: 2008-06-24
Publication date: 2010-08-04
Also published as: WO2009002982A3; WO2009002982A2

Abstract

The technology of the present invention relate generally to skin care compositions and methods, medication cosmetics or preparation, and preparation or using method.More specifically, the method of technology relate generally to preparation of the present invention and use compositions, medication cosmetics or preparation, described compositions, medication cosmetics or preparation comprise from gastropod, and promptly Helix Aspersa M ü ller etc. collects and purified unique combination thing.

Description

Gastropod biological fluid, preparation and process for purification and purposes

Related application

The application relates to following application and requires the rights and interests of following application: the title that on June 23rd, 2007 submitted to is BIOMEMBRANE PRODUCTS FOR SKIN RENEWAL, the U.S. Provisional Application 60/937 of WOUNDREPAIR AND SCAR REMOVAL, the title that on April 6th, 008 and 2008 submitted to is BIOCREAM PRODUCTS FOR SKIN RENEWAL, WOUNDREPAIR, the U.S. Provisional Application of AND SCAR REMOVAL (Express Mail Label No.EV 332942220US, Attorney Docket No.40064PIIA).Incorporate the full content mode by reference of aforementioned application into this paper.

Background of invention

The technology of the present invention relate generally to skin care compositions and methods, medication cosmetic composition and preparation or use their method.More specifically, technology relate generally to of the present invention utilizes from gastropod (gastropod) (that is, Helix Aspersa M ü ller etc.) and reclaims and skin care compositions and methods, medication cosmetic composition and preparation and their method of use of purified unique combination thing.

The organ of the body system maximum that skin is made up of the multilamellar epithelial tissue of protection underlying muscle and organ.Because it and environment junction, skin are resisted in pathogen and other environmental hazard at the protection health and are played an important role.Skin also plays an important role in the protection of the synthetic and vitamin B folate of other function such as isolation, thermoregulation, sensation, vitamin D.Therefore, must keep healthy skin and the rehabilitation that promotes impaired or compromised skin.Many conventional skin nursing products on current market contain and for example always do not help the skin rehabilitation, even may cause the chemosynthesis composition of deleterious side effect.

Different with many skin nursing products, the technology of the present invention is based on Chinese medicine (traditional Chinesemedicine), and the active component that wherein promotes skin repair and rehabilitation is from natural origin acquisition and refining.Glycoconjugate be to the general classification of the covalently bound sugar of other chemical species, have been found that it is a very important chemical compound in biology, is made up of many different types such as glycoprotein, glycopeptide, Peptidoglycan, glycolipid and lipopolysaccharide.In cell-cell interaction (comprising cell-cell recognition) and cell-matrix interaction, relate to glycoconjugate.What is interesting is that people are to having had new interest again by the excretory benefit of unique glycoconjugate of medicinal benefit that can provide of gastropod.

Gastropod or snail (snails) are the diversified classifications that belongs to Mollusca, have 60,000-75,000 known survival species.People have known that snail provides medicinal benefit several centuries, but recently the composition of snail, snail body part and/or snail secretions have been had darker understanding, prove that it has beneficial property.According to the described the technology of the present invention of the application, find unexpectedly, collect and purifiedly have forming of uniqueness by gastropod excretory biofluid of skin rehabilitation and regeneration properties that provides when stressed.This excretory biofluid can be used for many skin care compositions and methods and other preparation, is used to handle a large amount of dermatosis, for example wound, burn, scar, keratosis, psoriasis, acne, wrinkle and aging and skin injury.

Summary of the invention

On the one hand, the technology of the present invention provides the peculiar methods of collecting and making with extra care Gastropod biological fluid from the gastropod that lives.Described method comprises, for example, stimulates the step of gastropod, to increase the secretion of the biofluid of wishing.Stimulate the method for gastropod to comprise, for example, make gastropod be exposed to sonic vibration (sound vibration); Make gastropod be exposed to low high pressure (low hyperbaricpressure); Make gastropod be exposed to Spurs (thermal puncture); Make gastropod be exposed to physical stimulation (physical stimulus); Make gastropod be exposed to ionizing radiation (ionizing radiation); Make gastropod be exposed to gravity; Or reversing gastropod.Described method also comprises the step of collecting secreted fluid and centrifugal secreted fluid, to form supernatant.Described method also comprises the step of filtering supernatant to reclaim filtrate, and described filtrate is contained the glycosaminoglycans of the amount of raising, a kind of glycoconjugate.It should be understood that the technology of the present invention comprises the Gastropod biological fluid of collecting with described method.

On the other hand, the technology of the present invention provides one or more medication cosmetics (cosmeceuticals), and described medication toiletry bag contains the collection and the purified Gastropod biological fluid of effective dose.Recovery and purified Gastropod biological fluid (for example, filtrate or supernatant) can account for about by weight 1% to about 30% of the described compositions of the application.Selectively, Gastropod biological fluid can account for about 10% to about 20% of described one or more skin care compositions and methods of the application, preparation or medication cosmetics.Described compositions can be used for skin repair and regeneration, handles wound, acne and scar, and/or can be used as sunscreen, defying age protective skin cream, anti-wrinkle cream, moisturizing cream or fair complexion cream.Compositions of the present invention can also comprise the squalane of effective dose and other additives such as hyaluronic acid of effective dose.

Aspect another, the technology of the present invention provides the gastropod secretion fluid of biologic effective dose and delivery system that can application to skin.Delivery system can, for example, comprise cream frost (creams), gel, solution, oil, suspension, dispersion, ointment (ointments), film, paster (patches), powder, suppository, capsule, pill, soap and adhering skin glue (skin adhesive glues).

Aspect technology of the present invention another, provide medication cosmetics, compositions or the preparation of containing that promotes skin regeneration and rehabilitation at least about the biological filtrate of 15 weight % gastropods.

On the other hand, the technology of the present invention provides Gastropod biological fluid, and this Gastropod biological fluid comprises about 0.002% to about 0.1% alduronic acid; About 0.003% to about 0.5% aminohexose (hexozamine); About 0.001 to about 0.1% acetyl group thing (acetyl); About 0.001 to about 0.1% hydrosulphate (sulfate); About 0.02% to about 1.0% at least a mucopolysaccharide; About 0.05% to about 0.5% at least a fibroblast growth factor; About 0.01% to about 0.1% at least a enzyme; About 0.01% to about 1.0% hyaluronic acid; About 0.001% to about 0.2% at least a carrier of oxygen; About 0.005% to about 0.1% at least a high molecular weight protein; About 0.0001 to about 0.01% at least a trace element; About 0.005% to about 0.1% at least a high molecular anti-oxidant compounds (antioxidant compound); About 0.005% to about 0.1% at least a low molecular weight compound; And/or about 80% to about 98% water.

Aspect selectable, the technology of the present invention provides to be had at least about 1% to compositions, medication cosmetics or preparation at least about 30% Gastropod biological fluid.Described Gastropod biological fluid can be supernatant or the filtrate that contains the glycosaminoglycans of enriching quantity.

Aspect another, the technology of the present invention provides to be had about 10.0% and secretes fluidic compositions, medication cosmetics or preparation to about 25.0% gastropod.Described compositions, medication cosmetics or preparation also comprise about 0.5% to about 10.0% stearic acid; About 0.1% to about 1.0% glyceryl monostearate; About 1.0% to about 10.0% at least a oil; About 0.1% to about 1.0% octadecanol; About 1.0% to about 10.0% glycerol; About 0.02% to about 2.0% potassium hydroxide; And/or about 0.2% to about 2.0% antiseptic.

Aspect another, the technology of the present invention provides compositions, medication cosmetics or preparation, and described compositions, medication cosmetics or preparation have about 10% to about 20% gastropod secretion fluid; About 0.2% to about 2.0% Rosehips seed oil (rose hip seed oil); About 0.2% to about 2.0% olive oil emulsifying agent; About 0.5% to about 5.0% Sargassum extract; About 0.1% to about 1.0% hyaluronic acid; About 0.4% to about 4.0% chamomile extract; About 0.5% to about 5.0% sugared isomerate (saccharide isomerate); About 0.3% to about 3.0% Radix Glycyrrhizae extract; And/or about 0.3% to about 3.0% antiseptic.

Description of drawings

Fig. 1 is the image of SDS-PAGE gel, demonstrates with heparinlyase I, heparinlyase II or heparinlyase III to compare to the digestion of Gastropod biological fluid GAG with tester.

Fig.2 is one group of image of the tissue staining (histological staining) of diagram Helix Aspersa M ü ller mantle.Fig. 2-1 diagram WPA dyeing; Fig. 2-2 diagram nNOS dyeing; Fig. 2-3 diagram has the dyeing of the Dan Baijutang of carboxyl; The dyeing of Fig. 2-4 diagram carboxylic acid Dan Baijutang; Other glycoprotein dyeing of Fig. 2-5 diagram; Fig. 2-6 diagram δ-D-glucose and δ-D-mannose dyeing; The dyeing of Fig. 2-7 diagram oligosaccharide; Fig. 2-8 diagram δ-D-gal and (1-3)-D-Gal-Nac dyeing; Fig. 2-9 diagram δ-L-fucose dyeing; And Fig. 2-10 diagram N-acetyl group-D-galactosamine or the dyeing of D-galactose.

Fig. 3 is a line chart, is shown under citrate existence and the non-existent situation with Gastropod biological fluid (snail serosity (serum), the increase of fibroblastic cell proliferation of SJ) handling.

Fig. 4 illustrates the tissue staining of fibronectin assembling (fibronectin assembly) in the CHO-K1 cell of handling with the Gastropod biological fluid (SJ) of the technology of the present invention.

Fig. 5 illustrates at the tissue staining of handling the human skin flbroblast form in back (human dermal fibroblast morphology) with Gastropod biological fluid of the present invention.

Fig. 6 is the line chart of the antioxidation quality of diagram Gastropod biological fluid (snail serosity).

Fig. 7 is the line chart that illustrates the humidification quality of the humidifying liquid preparation that contains Gastropod biological fluid.

Fig. 8 illustrates the diagram to long-term (22 days) humidification effect of health volunteer's skin of the preparation that contains Gastropod biological fluid.

Fig. 9 is a stereo-picture, demonstrates that (C) and with the group of the anti-wrinkle cream processing that contains the technology of the present invention Gastropod biological fluid (F), anti-wrinkle cream is to the effect of wrinkle depth for D, E for A, B for the group of handling with contrast frost.Handle 0 day (A, D), handle 15 days (B, E) or handle 30 days (C measures in the time of F).

As described in the present application, the peculiar methods of Gastropod biological fluid and this Gastropod biological fluid purposes composition, medication cosmetics or preparation that useful skin nursing processing and treatment results for example are provided is collected and made with extra care to the technology of the present invention relate generally to from the gastropod that lives. Should can trigger based on natural composition, medication cosmetics and/or preparation the regeneration of damaged cell; Prevent or reduce scar, angling, the psoriasis scales of skin that peel off and all types of skin blemishes; Prevent or reduce the appearance of acne; Prevent or reduce the sign of wrinkle; And can replenish and/or humidification the skin lipid barrier. Described composition, medication cosmetics and/or preparation are based on natural component, preferably contain on a small quantity or do not contain chemicals, washing agent or filler. In this case, the unique combination thing that has this natural component is considered to (not fettered by any concrete theory although do not wish) and provides antimicrobial, protection, humidification, reparation and/or new property more to skin (comprise the cell inner structure of simulated skin and to the requisite component of normal skin function or component system).

In one embodiment, the technology of the present invention provides the method for collecting and making with extra care Gastropod biological fluid from the gastropod that lives. At least one advantage of the technology of the present invention is to reuse the gastropod collection of biological fluid, because the method is not preferably damaged gastropod. Gastropod secretes due-in collection and refining biofluid when stressed, with its skin membrane of protection, humidification, reparation and renewal. Said method comprising the steps of: the gastropod that thorn activates is to increase the biofluid secretion; Collect the fluid of secretion; The fluid of centrifugal secretion is to produce supernatant; With filtering supernatant to reclaim filtrate. This filtrate is contained one or more unique gastropod glycosaminoglycans of the amount of raising.

Gastropod used in this application is the member of Mollusca Gastropoda, comprise Cepaea (Helix) (with shell) and non-Cepaea (non-Helix) (no shell) member, its glandular secretion fluid is used as active component in the technology of the present invention practice. The gastropod that is fit to comprises, for example, Helix pomatia, Helixhortensis, Helix nemoralis, Helix cardidula, Helix tchthyomma (being also referred to as Helixcampylea), Helix fructicicola (being also referred to as Helix se uca), Helix strigella, Helixfruticum, Helix bidens, Helix arbostorum, Helix rotundata, Helix aculeata, Helix pulchella, Helix personata, Helix holoserica, Helix aperta, Helixparnassia, Helix alonensis, Helix candidissima, Helix pisana and Helixgualteviana. The widely discussion of relevant Gastropoda can be referring to Invertebrate Zoology (the 5th edition), 864p.Robert D.Barnes, (Saunders College Publishing, Inc., Troy, Mich., 1987). Particularly suitable gastropod is Helix Aspersa M ü ller. Selectively, the another kind of gastropod that is fit to is Helix pomatia.

At least a method of technology of the present invention comprises that the gastropod that thorn activates secretes to increase biofluid. The method of the gastropod that thorn activates can comprise does not damage gastropod alive, increases simultaneously the Gastropod biological fluid discharge rate, thereby makes gastropod again be used for any method that biofluid is collected. Stimulate and cause the gastropod increase by the biofluid secretion of mucoprotein (mucinous), albumen and the natural generation of salivary gland. These methods comprise, but be not limited to, make gastropod be exposed to acoustic vibration, make gastropod be exposed to low high pressure, make gastropod be exposed to Spurs, make gastropod be exposed to hypoxia condition (hypoxic conditions), make gastropod be exposed to gravity, the reversing gastropod with change it towards with make gastropod be exposed to physical stimulation.

Make gastropod be exposed to acoustic vibration and comprise methods known in the art, make gastropod be exposed to about 5kHz to about 25kHz, selectively about 10KHz is to about 20kHz, selectively about 15kHz is to the frequency of about 20kHz, continue about 1 second to about 60 seconds, selectively about 5 seconds to about 30 seconds, about 10 seconds more suitably. Can make gastropod be exposed to about 5kHz, about 10kHz, about 12kHz, about 15kHz, the audio frequency of about 20kHz continued at least about 5 seconds, at least about 10 seconds, at least about 15 seconds, at least about 20 seconds, to increase the biofluid secretion.

The particularly suitable method that makes gastropod be exposed to acoustic vibration comprises: use the ultrasonic generator UP 200H horntype (20kHz that is furnished with radial ultrasonic welding electrode S3 (radial Sonotrode S3) (3mm diameter), maximum amplitude is 210 μ m, maximum rated power is output as 460W), make gastropod be exposed to ultrasonic irradiation, continue about 10 seconds.

Selectively, can stimulate gastropod by making gastropod be exposed to low high pressure, to increase the output of waiting the biofluid collecting and make with extra care. Can use any suitable mode known in the art, comprise hyperbaric oxygen therapy (HBOT) chamber. The HBOT chamber that is fit to can make gastropod be exposed to about 3 pounds/square inchs (psi) to about 8psi, about 4psi (1.27ATA (absolute atmosphere) 8.92FSW) is to the pressure of about 7.35psi (1.5ATA 16.38FSW) more suitably, oxygen concentration is at least about 90%, more suitably at least about 95%, more suitably at least about 100%. Make gastropod be exposed to low high pressure, continue at least about 5 seconds, more suitably at least about 10 seconds, more suitably at least about 12 seconds, more suitably at least about 15 seconds, produce biofluid to stimulate gastropod.

Stimulate gastropod also can comprise the time that gastropod is reversed specified amount to increase the biofluid secretion. Can gastropod be suspended in midair by the gastropod afterbody, thereby the reversing gastropod makes its head towards ground, continues at least about 5 seconds, at least about 7 seconds, at least about 10 seconds, at least about 30 seconds, at least about 1 minute, at least about 1.5 minutes, at least about 2 minutes. For example, can use small-sized pliers to clamp the afterbody of gastropod and it was reversed about 10 seconds to about 2 minutes aloft. Can use suction pipe (straw) along gastropod abdominal surface collection of biological fluid.

Selectively, stimulating gastropod can comprise with the secretion of increase biofluid makes gastropod be exposed to gravity. Gravity causes the curved zone (sinus region) gastropod to be secreted such as cheek or the heart curved (buccal orcardiac sinus) biofluid to be increased. Can use syringe to draw biofluid to remove biofluid.

For increasing fluid secretion, also can make gastropod be exposed to ionizing radiation (x-ray or gamma-rays), continue about 0.5 second, about 1.0 seconds, about 2.0 seconds.Also can make gastropod be exposed to hypoxia condition to stimulate fluid secretion.Make gastropod be exposed to oxygen depletion or environment hypoxia, continue, at least about 12 seconds, at least about 15 seconds, to increase secretions output at least about 10 seconds.Can use any method of known oxygen depletion of those skilled in the art or environment hypoxia.

In another embodiment, stimulate gastropod can for example realize by making gastropod be exposed to physical stimulation to increase the biofluid secretion.The suitable mode of physical stimulation gastropod is centrifugal gastropod.Centrifugally under the condition of the shell that does not destroy band shell gastropod, carry out, perhaps if there is not the shell gastropod, then gastropod keep survival and the condition that do not weighed wounded under carry out.Gastropod is being enough to cause gastropod to secrete fluidic temperature and centrifugal a period of time of gravity (G).What stimulate that gastropod produces that the needed power of fluid is fit to is less than about 7G, be more suitable for less than about 6G, about 1G is about 6G extremely with being more suitable for, about 2G is about 5G extremely with being more suitable for, lasting about 2 minutes to about 10 minutes.A kind of suitable embodiment comprises, for example, or about 10 minutes of the centrifugal gastropod of about 2G.The centrifugal gastropod of following temperature: about 10 ℃ or higher, be more suitable for 15 ℃ or higher on ground, be more suitable for 20 ℃ or higher on ground, be more suitable for about 10 ℃ to about 35 ℃ of ground, be more suitable for about 20 ℃ to about 35 ℃.

Selectable centrifugal method be included in centrifugal during the fluctuation gastropod about 3 to about 4 times.Fluctuation comprises the centrifugal G power to hope of quickening, and speed reducing centrifugal also quickens centrifugal once more.The embodiment that is fit to of this method was included in the centrifugal gastropod of about 2G about 10 minutes, and implemented about 3 or about 4 secondary undulations during this period.

The method of collecting Gastropod biological fluid after stimulating gastropod can be to be familiar with the known any method of those skilled in the art.For example, can use suction pipe to draw biofluid from the gastropod abdominal surface.

Can be with the excretory biofluid of collected gastropod at about 200rpm to about 5000rpm, be more suitable for centrifugal at about 2000rpm, continue at least about 2 minutes, selectively at least about 5 minutes, selectively at least about 2 minutes to about 10 minutes, to remove any macroparticle and to produce supernatant.With the supernatant decantation and by the aperture is about 0.1 to filter to about 1 micron microfilter, to produce filtrate.Filtration can be carried out with the accelerated filtration process under pressure.Reclaim the filtrate that contains the active component in the Gastropod biological fluid then.

In some embodiments, also be included in from the method for the gastropod collection of biological fluid of living and make gastropod jejunitas before stimulating gastropod, selectively about 1 day to about 2 days at least about 1 day to about 5 days.Once more, do not wish to be bound by any concrete theory, make the amount of the toxin that may in secreted fluid, exist that the jejunitas by-product that is considered to reduce as the gastropod eaten food of gastropod produces.Selectively, if do not make gastropod jejunitas, then can remove toxin from secreted fluid by being familiar with the known method of those skilled in the art (for example, passivation toxin).

The technology of the present invention also provides and has contained with the supernatant of method for preparing and the Gastropod biological fluid of filtrate.Described in following embodiment, Gastropod biological fluid contains the mixture that oriented human skin provides the component of character such as useful rehabilitation and regeneration effect.When the delivery medium application to skin that use to be fit to, Gastropod biological fluid can, for example, promote immune system to remove dead and dying cell, the new cell of triggering for generating is rebuild dermal matrix by reparation, humidification and protecting skin component part.Described Gastropod biological fluid comprises about 0.001% to about 0.1% alduronic acid; About 0.003% to about 0.3% aminohexose; About 0.001 to about 0.1% acetyl group thing; About 0.001% to about 0.1% hydrosulphate; About 0.02% to about 5.0% at least a mucopolysaccharide; About 0.05% to about 0.5% at least a fibroblast growth factor; About 0.01% to about 0.1% at least a enzyme; About 0.01% to about 1.0% hyaluronic acid; About 0.001% to about 0.1% at least a carrier of oxygen; About 0.001% to about 0.1% at least a high molecular weight protein; About 0.0001 to about 0.01% at least a trace element; About 0.005% to about 0.1% at least a high molecular anti-oxidant compounds; About 0.005% to about 0.1% at least a low molecular mass antioxidants chemical compound; And/or about 80% to about 98% water.What be fit to is that described Gastropod biological fluid derives from Helix Aspersa M ü ller.

Described at least a mucopolysaccharide can comprise glycosaminoglycans (GAG) and the Dan Baijutang (PG) that contains sugar moieties (carbohydrate moieties), comprise chondroitin sulfate (chondroitin sufate, CS), dermatan sulfate (dermatan sufate, DS), heparin (heparin, HP) and Heparan sulfate (heparan sulfate, HS).What be fit to is, described mucopolysaccharide is the novel glycosaminoglycans that is produced by Helix Aspersa M ü ller, and molecular weight is about 29, and 000Da (measuring based on the viscometer method) evenly repeats the disaccharide structure and is

-2-acetyl group, 2-deoxidation-α-D-Glucopyranose.

-2-sulfo group-α-L-pyrans idose base alduronic acid (

-2-acetyl, 2-deoxy-α-D-glucopyranose

-2-sulfo-α-L-idopyranosyluronicacid).This polysaccharide is represented unique and is considered to new glycosaminoglycans.It is relevant with Heparan sulfate family with the heparin of glycosaminoglycans, but obviously is different from all known members of these kind glycosaminoglycans.The structure of this polysaccharide has adjacent N-acetyl glucosamine and 2-sulfo group-iduronic acid residue.The combination results of core protein and specific glycosaminoglycans have unique Dan Baijutang of accurate developmental pattern (precisedevelopmental patter).This glycosaminoglycans account for Limax soft tissue dry weight general about 3% to about 5%, hint has the important biomolecule effect to this organic existence.The gastropod glycosaminoglycans is securely in conjunction with bivalent cation, for example copper (II).Mucopolysaccharide account for that Gastropod biological fluid forms at least about 0.02%, at least about 0.2%, at least about 0.3%, at least about 0.5%, at least about 0.8, at least about 1%, at least about 2%, at least about 3% or at least about 4%.

Fibroblast growth factor (FGF) is the structurally relevant polypeptide growth factor of a class, at present by 23 member compositions with heparin-binding region.Known FGF works in vascularization, wound healing and fetal development.The described at least a fibroblast growth factor (FGF) of the technology of the present invention can be selected from: for example, and FGF1, FGF2, FGF3, FGF4, FGF5, FGF6, FGF7, FGF8, FGF9, FGF10, FGF11, FGF12, FGF13, FGF14, FGF15, FGF16, FGF17, FGF18, FGF19, FGF20, FGF21, FGF22, FGF23 or their combination.What be fit to is that at least a fibroblast growth factor that utilizes in the technology of the present invention practice is FGF1, FGF4, FGF7, FGF10 or their combination.Unique Gastropod biological fluid of the technology of the present invention contains by weight at least about 0.005%, at least about 0.01%, at least about 0.05%, at least about 0.08%, at least about 0.1%,, form at least about 0.5% at least a fibroblast growth factor at least about 0.2%.

Unique Gastropod biological fluid of the technology of the present invention also comprises one or more enzymes.Described enzyme can comprise, for example, and gelatinase, Dan Baijutang enzyme (proteoglycanases), collagenase (collagenases) or their combination.Gelatinase comprises any proteolytic enzyme of allowing organism gelatin hydrolysate alive, includes but not limited to MMP2 and MMP9.The Dan Baijutang enzyme belongs to a class matrix metalloproteinase (MMPs), and described matrix metalloproteinase (MMPs) is zinc dependency endopeptidase (zinc dependentendopeptidases).Their can degrade extracellular matrix proteins of all kinds.The Dan Baijutang enzyme that is fit to includes but not limited to MMP-23a, MMP-2, MMP23B, MMP-7, MMP-26.Collagenase comprises any enzyme that destroys the collagen peptide bond.Collagenase is considered to participate in destroying the extracellular structure in pathogen such as the antibacterial, therefore generates in response to tissue injury or immunne response.Described one or more enzymes account for by weight that Gastropod biological fluid forms at least about 0.005%, selectively at least about 0.01%, selectively at least about 0.02%, selectively at least about 0.05%, selectively at least about 0.08% or selectively at least about 0.1%.

Described Gastropod biological fluid also contains at least a carrier of oxygen, for example hemocyanin such as cupremia azurin.Hemocyanin is a high molecular oxygen carrier protein.Described oxygen carrier protein is considered to work in cell proliferation that reverses oxidative damage and promotion Skin Cell and regeneration.Gastropod biological fluid contains at least about 0.001%, selectively at least about 0.01%, and selectively at least about 0.02%, selectively at least about 0.05%, selectively at least about 0.08%, or selectively at least about 0.1% at least a carrier of oxygen.

Described Gastropod biological fluid also comprises at least a higher molecular weight protein.The higher molecular weight protein of finding in biofluid can comprise mucin (mucin) or mucous glycoprotein (mucusglycoproteins).Mucin or mucous glycoprotein are class polydispersity molecules, are designed to carry out multitask at the mucomembranous surface of whole body.Mucin is a high molecular epithelium glycoprotein, has high-load cluster oligosaccharide (clustered oligosaccharides), and described cluster oligosaccharide becomes the mode of glycosides to be connected to the tandem repeat peptide that is rich in threonine, serine and proline with O-.They are rich in cysteine residues, and described cysteine residues relates to the subunit that forms macromolecular complex crosslinked (sub unit crosslinking).They are also facilitated mucus gel barrier (mucus gel barrier) and are parts dynamic, mutual, the mucosa system of defense.For many years, to multinomial studies show that the mucous glycoprotein of many organs is carried out, these macromole are by combining by interchain disulfide bond and forming by the subunit of the further stabilisation of noncovalent interaction.What is interesting is, the Gastropod biological fluid of the technology of the present invention probably comprises by weight at least about 0.001%, selectively at least about 0.005%, selectively at least about 0.01%, selectively at least about 0.05%, or selectively at least about 0.08% at least a high molecular weight protein.

Described Gastropod biological fluid also comprises at least a trace element.Trace element comprises, for example, and copper (Cu), zinc (Zn), ferrum (Fe), calcium (Ca ²⁺) or their combination.Trace element accounts for the about 0.0001% of biofluid by weight, selectively at least about 0.0005%, and selectively at least about 0.001%, selectively at least about 0.005%, or selectively at least about 0.01%.

Described Gastropod biological fluid can also contain at least a high molecular anti-oxidant compounds.Antioxidant is the molecule that can slow down or prevent other molecular oxidation.Oxidation is with the chemical reaction of electronics from substance transfer to oxidant.Oxidation reaction can produce free radical, and free radical causes the chain reaction of infringement cell.Antioxidant stops these chain reactions by removing free radical intermediate, and suppresses other oxidation reaction by oxidation self.As a result, antioxidant often is a Reducing agent, includes but not limited to mercaptan or polyphenol.It is about by weight 0.005% that described Gastropod biological fluid contains, selectively at least about 0.008%, and selectively at least about 0.01%, selectively at least about 0.05%, or selectively at least about 0.1% at least a higher molecular weight antioxidant.In addition, medication cosmetics, compositions or the preparation that contains Gastropod biological fluid also can further comprise at least a other anti-oxidant compounds.Can use the anti-oxidant compounds of any suitable natural generation known in the art, include but not limited to olive oil derivant, oleic acid, Palmic acid, uric acid, carotenoid, vitamin Q (ubichinones), thioctic acid, vitamin C, vitamin E, phenolic compound, resveratrol, bata-carotene, selenium, high molecular antioxidant, low molecular mass antioxidants chemical compound, its derivant and their combination.Described medication cosmetics, compositions or preparation can comprise and account for total composition about 0.01% by weight to about 10.0%, are more suitable for ground about 0.1% to about 5%, are more suitable for the described anti-oxidant compounds on ground about 0.5% to about 3%.Described antioxidant can account for about 0.01%, about 0.05%, about 0.1%, about 0.2% of medication cosmetics, compositions or preparation by weight, about 0.5%, about 0.8%, about 1.0%, about 1.2%, about 1.5%, about 1.8%, about 2.0%, about 2.2%, about 2.5%, about 2.8%, about 3.0%, about 5.0%, about 7.0%, about 8.0%, about 10.0%.

It is about by weight 0.005% that described Gastropod biological fluid also contains, at least about 0.008%, and at least about 0.01%, at least about 0.05%, at least a low molecular mass antioxidants chemical compound at least about 0.1%.The low molecular mass antioxidants chemical compound is the pith of cell and organic antioxidation defense mechanism.Main low molecular mass antioxidants includes but not limited to uric acid, vitamin Q, thioctic acid, vitamin C and E, carotenoid and phenolic compound.

Described Gastropod biological fluid can be stored in use and final preparation/compositions/medication cosmetics first being processed.Can be at-10 ℃ to-30 ℃ approximately approximately, preferably approximately-15 ℃ to approximately-20 ℃ with this fluid quick freezing.Can store a large amount of biofluids more than 1 year by lyophilizing.Described biofluid is thermally sensitized, can be in about 60 ℃ to 80 ℃ degeneration, if be heated to approximately more than 90 ℃, then lose regeneration and antibacterial properties.Preferably, when when-20 ℃ of extremely about 70 ℃ temperature are stored approximately, this biofluid is stable in compositions.

In some embodiments, described Gastropod biological fluid can use with effective dose in the medication cosmetics." medication cosmetics " are the compositions that contains the combination of cosmetics and biological product and/or medicine, for example anti-wrinkle cream and sunscreen.Preferably, the medication cosmetics of the technology of the present invention provide beneficial property to skin.Beneficial property includes but not limited to keep healthy skin; Humidification; Rehabilitation and prevent cicatrixization; Treatment is by the skin of day photodamaged; Prevent premature aging and wrinkling; Induce skin regeneration and wound healing; Induce skin repair; Induce oxidative stress; Skin whitening pigmentation or the colour of skin is shoaled; Flexibility is organized in answer; Open the pore of obstruction; Treatment ingrown hair, pseudofolliculitis barbae (razor bumps), drying and photoaging; Antiinflammatory action; Alleviate acne scarsization; To anti-acne dermatitis (acne breakouts); Perhaps resist scalp and skin infection.The medication cosmetics that are fit to that can comprise the Gastropod biological fluid of the technology of the present invention preferably include defying age, crease-resistant, acne therapeutic agent, wound paster, trauma agent/ointment, Lightening compositions and antimicrobial compositions.

" effective dose " of Gastropod biological fluid provides the useful result's of hope amount.The effective dose of the Gastropod biological fluid that uses in the medication cosmetics of the technology of the present invention depends on employed delivery system and uses type.The effective dose of Gastropod biological fluid account for by weight the medication cosmetics at least about 1%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%.Gastropod biological fluid can account for about 1%, about 2%, about 4%, about 6% of medication cosmetics by weight, about 8%, about 10%, about 12%, about 14%, about 16%, about 18%, about 20%, about 22%, about 24%, about 25%, about 26%, about 28%, about 30%, about 32%, about 33%, about 34%, about 35%.

The medication cosmetics can comprise the Gastropod biological fluid of effective dose and delivery medium that can application to skin.The delivery medium that is fit to includes but not limited to cream frost, solution, gel, oil, suspension, dispersion, ointment, film, paster, powder, capsule, soap, suppository, its derivant or their combination.

The amount of Gastropod biological fluid can depend on employed delivery medium.In one embodiment, for example, the cream frost that is used for process facial, hand or health can comprise about 15% to about 20% Gastropod biological fluid.In another embodiment (immersion hand and foot or in shower the washing health liquid), compositions, medication cosmetics or preparation can contain has an appointment 1% to about 5% Gastropod biological fluid.(be used for specific region of skin is carried out the oil of Local treatment) in another embodiment, compositions, medication cosmetics or preparation can comprise about 30% to about 35% Gastropod biological fluid.In another embodiment, compositions, medication cosmetics or preparation can be the powder that is applied to wound area or specific region of skin, and this powder contains 15% to about 20% the Gastropod biological fluid of having an appointment.In another embodiment, compositions, medication cosmetics or preparation can be cover (mask) or the face shields (face mask) that covers big wound, and described cover or face shield comprise about 20% to about 25% Gastropod biological fluid.In another embodiment, the medication cosmetics can be the face of cover part or the film of body region or wound area, and this film comprises about 20% to about 25% Gastropod biological fluid.As controlling paster or the band (strip) that active component discharges, the medication cosmetics can comprise about 15% to about 25% Gastropod biological fluid.In another embodiment, the medication cosmetics can comprise pill or the capsule that is used for the oral administration active component, and described pill or capsule comprise the Gastropod biological fluid in medication cosmetics gross weight about 25% to about 30%.In another embodiment, the medication cosmetics can be the soaps of washing hand, face or health, and this soap comprises in composition total weight about 1% to about 10%, is more suitable for the Gastropod biological fluid on ground about 1% to about 3%.

In addition, described medication cosmetics can also comprise at least a olive oil or olive oil derivant.The olive oil derivant includes but not limited to oleic acid, Palmic acid and other fatty acid, Squalene, sterol (comprising plant sterol and fertility sterol (tocosterols)), butyl alcohol, hydroxytyrosol (hydroxytryrosol) (comprising oleocanthal and oleuropein), olive oil acid polyethylene glycol (4) ester (Peg-4olivate), surfactant anhydrosorbitol olive oil acid esters (sorbitan olivate), its derivant or their combination.The olive oil derivant contains effective anti-oxidants character, and humidification and immunostimulatory properties (immunestimulating properties).Described compositions comprises about by weight 0.01% to about 10%, is more suitable at least a olive oil derivant on ground about 0.5% to about 5%.Described at least a olive oil derivant can account for about 0.08%, about 0.1%, about 0.2%, about 0.3%, about 0.5% of medication cosmetics of the present invention, compositions or total formulation weight amount, about 0.8%, about 1.0%, about 1.2%, about 1.5%, about 1.8%, about 2.0%, about 2.2%, about 2.3%, about 2.4%, about 2.5%, about 2.6%, about 2.8%, about 3.0%, about 3.2%, about 3.4%, about 3.6%, about 4.0%, about 4.2%, about 4.5%, about 4.8%, about 5.0%, about 7.0%, about 10.0%.Described olive oil derivant can compatibly be the combination of olive oil acid polyethylene glycol (4) ester, squalane and anhydrosorbitol olive oil acid esters.In a kind of suitable embodiment, described at least a olive oil derivant comprises about 0.2% to about 2.0%, is more suitable for olive oil acid polyethylene glycol (4) ester on ground 0.8% to about 1.0%; About 0.2% to about 2.0%, is more suitable for the squalane on ground about 0.8% to about 1.0%; With about 0.2% to about 2.0%, be more suitable for the anhydrosorbitol olive oil acid esters on ground about 0.8% to about 1.0%.

In some embodiments, described medication cosmetics also comprise about 0.01% to about 10%, are more suitable for ground about 0.1% to about 2%, are more suitable for the squalane on ground about 0.8% to about 1.2%, based on the gross weight of medication cosmetics, compositions or preparation.Squalane is a natural organic-compound, mainly derives from shark liver oil or olive oil, but also is present in plant source such as three-coloured amaranth seed, Testa oryzae, Fructus Hordei Germinatus and Fructus Canarii albi.Squalene is used as natural humidizer and antioxidant in the medication cosmetics, its skin permeation stays greasy residue rapidly and not.

In some embodiments, described medication cosmetics also comprise hyaluronic acid.Hyaluronic acid can account for composition total weight about 0.01% to about 10%, be more suitable for ground about 0.1% to about 5%, be more suitable for ground about 0.5 to about 2%, be more suitable for ground about 0.8% to about 1.0%.Hyaluronic acid is not Sulfated glycosaminoglycans, is distributed in connective tissue, epithelial tissue and nervous tissue widely.It is one of key component of extracellular matrix, facilitates cell proliferation and migration, participates in many cell surface receptors interactions and relates to tissue repair.Hyaluronic acid has proved effective free radical scavenger, and also can be effective when removing the oxidant that is caused by ultraviolet radiation.It works in induced tissue rehabilitation (particularly after operation is as cataract operation).In compositions, hyaluronic acid can work organizing rehabilitation and induce in the oxidative stress on the skin.In some embodiments, make the combination of hyaluronic acid and Squalene and Gastropod biological fluid, to form the medication cosmetics that need.

In other embodiments, described medication cosmetics, compositions or preparation can comprise antiseptic.Antiseptic is to be added into medication cosmetics, compositions or preparation to avoid the composition of the infringement that caused by air and photoconduction to prevent microorganism (comprising antibacterial, virus, yeast and fungus) growth and protection medication cosmetics.Described medication cosmetics can comprise about 0.01 to about 10%, are more suitable for ground about 0.1 to about 5%, are more suitable for ground about 0.5% to about 5%, are more suitable for the antiseptic on ground about 0.8% to about 1.2%, based on the gross weight of compositions.Particularly suitable antiseptic comprises phenoxyethanol, ethylhexyl glycerol, its derivant or their combination.Phenoxyethanol is an organic compound, the glycol ethers antibacterial.Ethylhexyl glycerol (1-(2-ethylhexyl)-glycerin ether) is the antiseptic potentiator, bacteria growing inhibiting and be gentle wetting agent and skin isostearyl glyceryl pentaerythrityl ether.Can use any suitable antiseptic that is used for cosmetics known in the art.Other selectable antiseptic comprises p-Hydroxybenzoate.In addition, antiseptic can be the combination of plant extract.The plant extract that is fit to comprises, for example, leaf of Herba Origani extract (organumvulgare leaf extract), Thymi Serpylli Herba extract (thymus vulgaris extract), Cortex Cinnamomi (cinnamomum zeylanicum bark extract), leaf of Herba Rosmarini Officinalis extract (rosmarinus officinalisleaf extract), lavender flower extract (lavandula augustifolia flower extract), Fericarpium Citri Limoniae extract (citrus medica limonum peel extract), Folium Menthae extract (menthe piperitaleaf extract), goldenseal root extract (hydrastis Canadensis root extract), Olive leaf P.E (olea europaea leaf extract), its derivant or their combination.

Described medication cosmetics, compositions or preparation can also comprise at least a solvent.Solvent is to make solid, liquid or the gas dissolving liquid in the gained solution.The solvent that is fit to includes but not limited to water, glycerol, alcohol, its derivant and their combination.Glycerol is colourless heavy-gravity sugar alcohol, and it also can easily be dissolved in alcohol or the water.Glycerol also is height " hygroscopic ", this means that it is from absorption of air water.Glycerol provides following effect to compositions: improve smoothness, provide lubricated and as wetting agent.The amount of glycerol depends on the delivery system of medication cosmetics, compositions or preparation.What be fit to is, described medication cosmetics, compositions or preparation comprise and account for gross weight about 0.1% to about solvent of 99%, selectively about 10% to about 90%.The medication cosmetics can comprise and account for compositions by weight, medication cosmetics or preparation at least about 0.1%, about 1.0%, about 5%, about 8%, about 10.0%, about 12.0%, about 15.0%, about 18.0%, about 20.0%, about 22.0%, about 24.0%, about 25.0%, about 28.0%, about 30.0%, about 32.0%, about 35.0%, about 36.0%, about 38.0%, about 40.0%, about 42.0%, about 44.0%, about 48.0%, about 50.0%, about 55.0%, about 58.0%, about 60.0%, about 62.0%, about 64.0%, about 66.0%, about 68.0%, about 70.0%, about 72.0%, about 74.0%, about 76.0%, about 78.0%, about 80.0%, about 82.0%, about 84.0%, about 86.0%, about 88.0%, about 90.0%, about 92.0%, about 94.0%, about 96.0%, about 98.0% solvent.

In some embodiments, described medication cosmetics, compositions or preparation can comprise abrasivus.The abrasivus that is fit to provides the result that comes off of dead cell or tissue and other chip, and does not damage skin.The abrasivus that is fit to is known in the art, includes but not limited to corudrum oxide.Described medication cosmetics, compositions or preparation can comprise about by weight 0.0005% to about 0.05%, are more suitable for the abrasivus on ground about 0.001% to about 0.01%.

In some embodiments, described medication cosmetics, compositions or preparation can comprise essence (aromatic essence).Essence can provide fragrance to preparation, it includes but not limited to, for example, vanilla, jasmin essence, violet compound, rose essence, lavandula angustifolia essence, Chamomile essence, flavoring peach essence, strawberry essence, cocoanut flavour, green tea essence, Herba Menthae essence, orange essence etc.Described medication cosmetics, compositions or preparation can comprise about 0.005%, selectively about 0.01%, selectively about 0.05%, selectively about 0.1%, selectively about 0.5%, selectively about 0.8%, selectively about 1.0%, or selectively about 2.0% essence.

In a kind of specific embodiment, described medication cosmetics, compositions or preparation can comprise about 5.0% to about 10.0% stearic acid, about 0.1% to about 1% glyceryl monostearate, about 1% to about 10% at least a oil, about 0.1% to about 1% octadecanol, about 5% to about 15% glycerol, about 10% to about 20% Gastropod biological fluid, about 0.1% to about 1.0% potassium hydroxide, about 0.1% antiseptic (comprising the combination of phenoxyethanol and ethylhexyl glycerol) to about 2.0% essence and about 0.1% to about 2.0%.Described one or more oil can comprise white oil, silicone oil, its derivant or their combination.What be fit to is, described compositions, medication cosmetics or preparation comprise at least a oil, described at least a oil contain have an appointment 4.0% to about 5.0% white oil and about 1.0% to about 2.0% silicone oil, based on the gross weight of compositions.These medication cosmetics can also comprise the water of capacity, so that compositions reaches about 1000 grams.

In the another kind of specific embodiment, described medication cosmetics, compositions or preparation can comprise about 0.1% to about 1.2% olive oil acid polyethylene glycol (4) ester, about 0.1% to about 1.2% anhydrosorbitol olive oil acid esters, about 0.1% to about 1.2% squalane, about 10.0% to the biological serosity of about 20.0% gastropod, about 0.1% to about 2.0% hyaluronic acid, about 10% to about 20% glycerol, about 0.1% to about 2% antiseptic (for example, phenoxyethanol and ethylhexyl glycerol) and about 0.1% to about 1%corundrum oxide, based on the gross weight of compositions.

In another embodiment, described medication cosmetics, compositions or preparation comprise about 2.4% to about 3.0% at least a olive oil derivant, about 10% to about 15% Gastropod biological fluid, about 0.8% to about 1.2% hyaluronic acid, about 10.0% to about 15.0% glycerol and about 0.8% to 1.2% antiseptic (for example, phenoxyethanol and ethylhexyl glycerol), based on the gross weight of compositions.What be fit to is that these embodiments comprise water, so that weight of formulation reaches about 1000g.

In another specific embodiment, described medication cosmetics, compositions or preparation can comprise about 0.01% to about 5.0%, are more suitable for the Rosehips seed oil on ground about 0.1% to about 2.0%, based on the gross weight of compositions.The Rosehips seed oil is rich in the essential fatty acid of vitamin A precursor and nourishing and humidification skin.Described medication cosmetics, compositions or preparation can also comprise about 5% to about 20.0%, and the Gastropod biological fluid filtrate, about 0.1% that is more suitable for ground about 10% to about 15% is to about 10.0% olive oil derivant, based on the gross weight of compositions.The olive oil derivant comprises emulsifying agent, surfactant and the squalane of the lipid content that replenishes skin and their derivant, its include but not limited to the application described those.Described medication cosmetics, compositions or preparation can also comprise about 0.5% to about 5.0%, are more suitable for the Sargassum extract on ground about 1.5% to about 2.5%, based on the gross weight of compositions.Sargassum extract provides the trace element that promotes the cell growth.Described medication cosmetics also comprise about 0.01% to about 10.0%, and the hyaluronic acid, about 0.1% that is more suitable for ground about 0.1% to about 2.0% is more suitable for the Chamomile on ground about 1.0% to about 4% to about 10%.Chamomile provides antiinflammatory property, is useful for sensitive skin.In addition, described medication cosmetics, compositions or preparation can also comprise about 0.1% to about 10%, are more suitable for the sugared isomerate on ground about 2.0% to about 5.0%, based on the gross weight of compositions.The sugar isomerate comprises the promotion carbohydrate complexes similar to complex that find skin moisturizing (carbohydrate complex) in skin.In addition, described preparation can comprise Radix Glycyrrhizae extract (Glycyrrhizainflata extract), and it has antioxidation, antiinflammatory, irritation, antimicrobial and sebum and regulates active.Radix Glycyrrhizae extract can account for composition total weight about 0.5% to about 10%, be more suitable for ground about 1.0% to about 5.0%.In addition, described compositions can comprise about 0.1% to about 10%, is more suitable for the antiseptic derived from natural plant extracts on ground about 1% to about 5%, based on the gross weight of compositions.These plant extracts provide antimicrobial, antibiotic, antifungal, anti-yeast and/or antiviral properties.The plant extract that is fit to includes but not limited to Adeps Bovis seu Bubali (Adeps Bovis seu Bubali) leaf extract, Herba thymi vulgaris (Herba thymi vulgaris) extract, Cortex Cinnamomi (Cortex Cinnamomi) peel extract, Herba Rosmarini Officinalis (Herba Rosmarini Officinalis) leaf extract, lavandula angustifolia (lavandula angustifolia) flower extract, Fructus Citri Limoniae (Fructus Citri Limoniae) peel extract, Herba Menthae (Herba Menthae) leaf extract, goldenseal (goldenseal) root extract and Fructus Canarii albi (Fructus Canarii albi) leaf extract.

Medication cosmetics, compositions or the preparation of the technology of the present invention can also comprise the gastropod living things system of effective dose and derived from the composition of Chinese medicine, to produce new preparation.The composition that is fit to derived from Chinese medicine includes but not limited to that oil (for example, Oleum Rosae Rugosae, emulsion aburagi fruit oil, Fructus Maydis oil, Semen Tritici aestivi germ oil, fibert oil, Oleum sesami), sugar and sugar derivatives (for example, dextrose, AHA ('alpha '-hydroxy acids), glycolic, glycerol), plant and fruit elite (for example, Semen Ginkgo elite, Flos Tulipae Gesnerianae elite, Aloe, Radix Hamamelidis Mollis elite, Herba thymi vulgaris elite, salicylic acid, Willow bark elite, carbamide and Radix Glycyrrhizae elite (essenceofcarbamide and liquorice), orange essence, blackberry elite); Medicinal herbs (for example, Ganoderma, Radix Ginseng); Fruit, nut, vegetable and their derivant are (for example, red guava, Semen phaseoli radiati, Semen Castaneae, Fructus Rubi, ellagic acid, Sargassum, aqua aurantii florum, linseed acid (flaxseed acid), Semen Helianthi), vitamin (for example, vitamin C, vitamin E, ascorbic acid, ubiquinone); Detergent (for example, sodium laurate (sodium lauryl) or laureth sulfuric ester (laureth sulfate), triethanolamine lauryl sulfate (TEA-laurylsulfate)); Emulsifying agent (for example, cocoyl diethanolamine); Fungus and fungus derivant (for example, kojic acid); With other material (for example, C _14-16Thiazolinyl sodium sulfate (Sodium C14-16 olefinsulfate C14-16), triethanolamine, Mel elite (honey essence), left-handed-C (levorotation-C), amide, jobs of tears, arginine, lactic acid, carbamide etc.).These preparations can be used for various skin nursing item for disposal, include but not limited to, wound paster, operation back rehabilitation film, regeneration elite (rejuvenationessence), antibiotic medicine (refining conditioning frost (purification conditioning cream)), anti--acne cream, anti-wrinkle cream, humidification facial cleansing soap, shower gels, hand cream (hand cream), fair complexion cream, puerperal cosmetics, hemorrhoid thrombolytics, uvioresistant skin nursing frost, anti-drying skin nursing frost, the anti-rehabilitation frost (anti-burn healing cream) of burning).

A kind of specific embodiment of fair complexion cream comprises Gastropod biological fluid, vitamin C, HUANGLONG Radix Gentianae (yellow gentian grass), kojic acid, at least a antioxidant (for example, green tea) and at least a plant extract.The plant extract that is fit to comprises Pyrusussuriensis (sorbic), occident pear, olive fruits, Semen Ginkgo, Folium Vaccinii vitis-idaeae (ursolic), external Fagus sinensis Oliv. (foreign beech), Sargassum, Betula platyphylla Suk., mulberry, Radix Ginseng extract etc.

A kind of specific embodiment of sun-proof medication pomade frost comprises Gastropod biological fluid and is selected from following at least a ultraviolet blocker (UV-blocking agent): PABA (for example, para-amino benzoic acid, dimethylaminobenzoic acid monooctyl ester (Padimate O), dimethylaminobenzoic acid pentyl ester (PadimateA), glyceryl PAA (Glyceryl PAA)), salicylate (for example, heliophan, ethylhexyl salicylate, trolamine salicylate (trolamine salicylate)), cinnamate (for example, octyl methoxycinnamate, cinoxate); Benzophenone (for example, oxybenzone (oxybenzone), dioxybenzone (dioxybenzone), sulisobenzone), ortho-aminobenzoic acid (anthranilate), methyl 2-aminobenzoate (methylanthranilate), physical agent (for example, xinc oxide 4, red vaseline (redpetroleum)) and their combination.

A kind of specific embodiment of the white medication cosmetics of the therapeutic of the technology of the present invention crease-resistant (wrinkle resistant) comprises the combination of Gastropod biological fluid and for example following material: Semen Ginkgo, the green tea factor (green teafactors), Aloe, biological peptide-C1 (biopeptide-C1), vitamin E, placenta prime, the ARL defying age factor (ARL against aging factor) and Semen Armeniacae Amarum.

A kind of specific embodiment that the therapeutic of the technology of the present invention is removed acne medication pomade frost comprises, for example, Gastropod biological fluid, Radix Gentianae Bulbus Allii extract (gentian garlic extract), hop oil, chamomile extract, Helianthi extract, tea tree oil and Flos Inulae.

Embodiment above any can also comprise the wetting agent that makes dermal sensation soft and smooth.These wetting agent can comprise PEG (Polyethylene Glycol), polysorbate (polysorbates), propylene glycol, polypropylene glycol, butanediol, hexanediol, isopropyl myristate, its derivant or their combination.Embodiment above any can also comprise other antiseptic to prevent antibacterial, mycete (mold fungal), parasite, yeast or other growth of microorganism.The antiseptic that is fit to can comprise, for example, and quaternary ammonium 15 (quaternium15), imidazolidinyl urea, ethylparaben, butyl p-hydroxybenzoate, propyl p-hydroxybenzoate, methyl parahydroxybenzoate.

A kind of specific embodiment that the water conservation cream medicine of the technology of the present invention is treated cosmetics comprises Gastropod biological fluid, glycerol, HA (hyaluronic acid), PCA-NA (L-pyrrolidone sodium carboxylate or L-sodium pyroglutamate), NMF (nature moisturizing factor), neural thiamine (nerve thiamine) and collagen protein, carbamide, lactic acid, chitosan, Aloe and Sargassum extract.Other additional component that is fit to comprises, for example, propylene glycol, polypropylene glycol, butanediol, hexanediol, three (sad, capric acid, lauric acid) glyceride, annular dimethyl polysiloxane, polydimethylsiloxane, fatty acid, hyaluronic acid, pyrrolidone sodium carboxylate, collagen, ceramide, elastin laminin, lecithin, its derivant or their combination.

Described medication cosmetics also can the form with the therapy paste frost prepare described in following embodiment.This therapy paste frost can be used for (i) and handle polytype burning, and comprises heat and chemical burn, (ii) prevention or processing radiodermatitis and (iii) prevention or processing sunburn.

In another embodiment, the medication cosmetics of the technology of the present invention can be nourishing face or the hand cream frosts that uses on normal (normal), dryness or oily skin.Described Gastropod biological fluid is considered to repair epithelial tissue and rawhide skin more.This cream frost also is considered to as cracking-off agent, removes the dead skin layer (epithelium vitreous layer) of outside and promotes new skin growth.The use in this cream frost of pantothenic acid and heparin is chosen wantonly, but preferably uses, because they improve rehabilitation and osmosis.This cream frost makes the skin anxiety and rejuvenates, and can be used to handle SLE, eczema, dermatitis and very exsiccant skin symptom.

In another embodiment, the technology of the present invention medication cosmetics can be purification and the wetting liquids that is used for dryness, normal or oily skin.The cleaning liquid skin of gained and make skin smooth improves the transparency of the colour of skin.Also (for example, powder) foundation cream (base) protects skin to avoid the illeffects of cosmetic powder (this cosmetic powder is 1 mineral composition by hardness number on the Mohr scale) as cosmetics for it.The preparation that is fit to comprises about 2ml Gastropod biological fluid in about 200g output aggregate.What be fit to is, it is about 0.5% that described purification and wetting liquid preferably comprise, selectively about 1.0%, selectively about 2%, selectively about 5%, selectively about Gastropod biological fluid of the present invention of 10%, selectively about 15%.

In another embodiment, the technology of the present invention medication cosmetics can be to be in harmonious proportion and clean liquid.Recommendation is used in this liquid on the tender and lovely skin and exists on the skin of acne.It produces very frank sensation and demulcent quiet sensation.

In another embodiment, the technology of the present invention medication cosmetics can be the anti-wrinkle creams that is used for every day or uses weekly.This cream frost is easy to use, and if only use existing on the zone of wrinkle, be best.It is effectively that described Gastropod biological fluid active component is considered to for reducing wrinkle, and other component produces astriction simultaneously.After using, the effect of described cream frost continues at least one week at every turn.

In addition, the technology of the present invention medication cosmetics can be as sunscreen or sunburn inorganic agent.What be fit to is that described sunscreen must be used before being exposed to daylight.For the sunburn inorganic agent, described cream frost can be used once in per six hours, up to transference cure.In addition, described cream frost can be used as Ri Guang ﹠amp; The pigment correction agent (pigment corrector) (this pigment correction agent comprises Gastropod biological fluid and Rumex botanical extract) of aging speckle and melasma and under situation without any side effects the calm over-drastic bionical peptide (biomimetic peptide) of pigmentolysis.

Other medication cosmetics according to the technology of the present invention imagination comprise family's miniature dermabrasion cream frost (home microdermabrasion cream), described family miniature dermabrasion cream frost contains skin regeneration activator and crystallite, in wiping coarse part, dull skin (dull skin), damaged tissues and scar and allowing that enzyme in the serosity decomposes impaired albumen and in depth penetrates to the skin; No oleogel is with prevention and processing acne, SLE and scar; With the cream frost that is used for coarse, old health scar, conceived tractive vestige (pregnancy stretch marks), all constituents that are used to rebuild the engineering properties (comprising compactness, toughness, pliability and elasticity) of being responsible for skin with the aminoacid that decomposes dark scar tissue and discharge them.

The custom-designed excipient that is used to preserve biological substance and be used for dermatosis and cosmetics inorganic agent is used for and can (be positioned at Iowa City, the Younger that Iowa) is purchased from Pharmacom ^TMBiocream.An advantage of this excipient is, its accept nearly all other active component and give the stability of compositions the best.This preferred excipient is that about 900 to about 1300 Polyethylene Glycol mixture, spermol, glyceryl monostearate, the mineral oil of selection, the calcium carbonate that is the micropowder form, the zinc oxide that is the micropowder form and water are made by molecular weight ranges.This excipient is made up of following material basically: about 10% to about spermol of 17%, preferably about 12.0% to about 15.5% and/or its by-product such as laurate, myristinate and adipate ester; About 20% to about Polyethylene Glycol mixture of 50%, preferably about 30% to about 50% (for example, PEG400,1500 and 4000 mixture); About 3.5% to about glyceryl monostearate of 5.8%, preferably about 4% to about 6%; About 7% to about mineral oil of 12%, preferably about 8% to about 10% (preferably paraffin oil); About 5% to about calcium carbonate that is the micropowder form of 8%, preferably about 5.5% to about 7%; About 0.3-0.6%, the preferably zinc oxide that is the micropowder form of 0.32-0.40%; With the water of effective dose, usually as many as about 35%, preferably about 16% to about 30%.The amount of water base (aqueous base) should make and be enough to obtain the compositions of required denseness.If water base amount is higher than 35%, then should use antiseptic.Described water base water, saline solution [for example, not containing 0.9% the sodium chloride solution of giving birth to hot material (pyrogenous matter)] or the physiology serosity of comprising.Described percent be by weight and summation be 100%.Percent can easily be regulated with minimum experiment (minimalexperimentation), so that the creamy consistencycreamlike consistency that needs to be provided.Described excipient is tasteless and white, and not influenced by ultraviolet radiation, x-ray and gamma-rays.It can use in preparation cream frost, emulsion and unguentum etc.It is considered to not have ill-effect (even when going through long-time section when using) or do not produce adverse effect such as allergy or carcinogenic effect.This excipient also is considered to promote the percutaneous absorption of active component, is not damaging between-20 ℃ to about+70 ℃ approximately, and be not subjected to humidity effect, therefore can use in many weather limit.In addition, this excipient is stable, not influenced by the gamma-radiation of decimetric wave, ultraviolet radiation, 1.3MeV and photon.Preferably, not influenced by hold-up vessel.The importance of this excipient is not based on external component, and is based on their character, and this character is the result of a large amount of experimental preparations.

For described water base greater than 35% situation, the optional member that can use in the medication cosmetics comprises antiseptic such as methyl parahydroxybenzoate, oxidation retarder such as sodium sulfite and other composition that comprises usually in therapeutic composition.

The optional member that can use in compositions, medication cosmetics or the preparation of the technology of the present invention comprises mineral oil such as paraffin oil, Oleum Testudinis or other oil, and consumption is about 1% to about 10%, is preferably about 2%; Boric acid, consumption are about 0.1% to about 0.6%, are preferably about 0.3%; Chlorophyll, consumption are about 0.25% to about 1.5%, are preferably about 1.0%; Formalin (for example, about 30% concentration), consumption are about 0.3% to about 3%, are preferably about 0.4%; Glycerol, consumption are about 0.1% to about 4.0%, are preferably about 0.5%; Heparin, consumption are about 0.01% to about 0.4%, are preferably about 0.1%; Lanoline, consumption are about 0.1% to about 6%, are preferably about 0.5%; Menthol, consumption are about 0.25 to about 2.5%, are preferably about 1%; Pantothenic acid, consumption are about 0.01% to about 0.5%, are preferably about 0.1%; Aluminium potassium sulfate, consumption are about 0.1% to about 1.5%, are preferably about 0.4%; Copper sulfate, consumption are about 0.01% to about 0.3%, are preferably about 0.03%; Vitamin C, consumption are about 0.01% to about 0.5%, are preferably about 0.2%; Or metal iodide (metallic iodine), consumption is about 0.1% to about 1.1%, is preferably about 0.6%.Above percent be by weight, and the gross weight of cosmetic composition is by weight 100%.

By reference following examples, technology of the present invention and its advantage will be understood better.Provide these embodiment to describe the specific embodiment of the technology of the present invention.The inventor is scope and the purport by providing these embodiment to limit the technology of the present invention not.Those skilled in the art will be appreciated that the four corner of technology of the present invention comprises the purport that any change (alterations), modification (modifications), possibility (alternatives) or the equivalent (equivalents) of the claim of this description of enclosing and these claim are limited.

Embodiment

Embodiment 1: collect and refining Gastropod biological fluid

Helix Aspersa M ü ller originates in France, eats the high protein diet growth.With Helix AspersaM ü ller Limax centrifugal 10 minutes, and carry out 3 secondary undulations with 2G.Fluctuation is implemented by the following method: make centrifuge accelerate to 2G, make centrifuge decelerates and make centrifuge accelerate to 2G once more.At each fluctuating period, make centrifuge accelerate to 2G, slowing down also accelerates to 2G once more.After centrifugal, be used to remove fluidic suction pipe and collect the fluid exudates of Limax, and collect and be suitable in the centrifugal pipe from Limax.With fluid exudates with centrifugal 10 minutes of 2000rpm to remove any macroparticle.With the supernatant decantation in the cylinder that contains 0.1 μ m microfilter.Seal described cylinder with closure, described closure is connected to compressed air system to promote filtration.Then with the Gastropod biological fluid of filtrate as technology of the present invention.

Embodiment 2: the decomposition of components of Gastropod biological fluid

In order to estimate the chemical composition of Gastropod biological fluid, Helix Aspersa M ü ller Limax is handled GAG (glycosaminoglycans) concentration to measure them.Remove Crusta Limax, and use three acetone extracts of 24-hour whole software defat.Use razor blade that fat free dried Limax is cut into fine powder.Make that about 4g is that do, defat, comminuted powder is suspended in the 0.05M sodium carbonate buffer solution (sodium carbonated buffer) (pH 9.2) of 40ml.Add 2ml protease (Alcalase, 2.4 Anson units (Anson units)/g), then with suspension with 200rpm 60 ℃ of shakes 48 hours.Digestion mixture is cooled to 4 ℃, and adds the ultimate density of trichloroacetic acid to 5%.With sample mix, left standstill centrifugal 20 minutes then 10 minutes with 8000 * g.Reclaim supernatant by decantation.5% potassium acetate in ethanol of three volumes is added into the supernatant of a volume.After mixing, will be suspended in 4 ℃ of storages and spend the night, centrifugal 30 minutes then with 8,000 * g.Abandon supernatant, and with dehydrated alcohol (absolute alcohol) washing precipitate.Precipitate (1g) is dissolved among the 0.2MNaCl of 40ml and centrifugal 30 minutes, and abandons insoluble material with 8,000 * g.Add the cetyl pyridinium chloride (5%) of 0.5ml to the material of gained, and the precipitate by centrifugal collection gained.This precipitate is dissolved among the 2.5M NaCl of 10ml then, adds 5 volume of ethanol, and with precipitate with 10,000 * g centrifugal 30 minutes.Precipitate is dissolved in the water once more and to the water dialysis of 100 volumes, and will be through the part lyophilizing of dialysis, to obtain the GAG gastropod fluid as white powder filtrate (white powder filtrate) of 0.18g.

The analysis of physical of complete polysaccharide

Will be from the polysaccharide GAG of Gastropod biological fluid (200 μ g) at P ₂O ₅There is down finish-drying in a vacuum and uses and have Teflon and be dissolved in the 1M HCl methanol solution of 0.5ml as the screw capped tube (screw-cappedtube) of the lid of liner.Make nitrogen pass through the solution bubbling 15 seconds, then test tube is sealed.After 80 ℃ of Methanol Decomposition 24 hours, by adding 0.15ml pyridine neutralizing acid solution.Carry out N-acetylation once more (Re-N-acetylation) by adding the 0.1ml acetic anhydride.This mixture was kept about 1 hour in room temperature.Then, evaporate sample solutions with nitrogen current at 35 ℃.With residue through P ₂O ₅Vacuum drying 16 hours.At last, with 50 μ l silylating reagents (pyridine/N, O-is two-(trimethyl silyl) trifluoroacetamide, 1: 2 (v/v)) with sample room temperature trimethyl silylization 30 minutes.Use the capillary column AT-1 of Alltech Associates (being positioned at DeerfileIL), 0.53mm * 30-m (1.5-μ m thickness), at the Shimadzu (Kyoto that is furnished with Shimadzu Chromatopac CR501 integration recorder, Japan) gas chromatograph (model GC-14A has flame ionization detector) enterprising circulation of qi promoting phase chromatography (GC) composition analysis.Injection port temperature and detector temperature are respectively 270 ℃ and 280 ℃.For the analysis of mixture of monosaccharides, with furnace temperature be set at 120-260 ℃ (10 ℃/min).

The lyases of GAG is handled the analysis of formed oligosaccharide

By capillary electrophoresis (CE), the Dionex Capillary Electrophoresis system that use is furnished with the high voltage power supply that can carry out constant or gradient voltage control (can be purchased from the DionexCorporation of Sunnyvalle, has advanced computer interface, model I), (Sunnyvale, fused silica capillary analysis CA) is passed through the disaccharide of heparinlyase generation separately and the compositions of oligosaccharide by complete polysaccharide to use DionexCorporation.Operate the CE system by use sample at the negative electrode place with the reversed polarity pattern, and move with 20mM phosphoric acid (as mentioned above, being adjusted to pH 3.5) with the 1M sodium hydrogen phosphate.Use the 0.5M sodium hydroxide of 0.5ml before use,, use the electrophoretic buffer (running buffer) of 0.5ml that capillary tube (68cm is long for 75-μ m internal diameter, 375-μ m external diameter) is manually washed then then with the distilled water of 0.5m1.By hydrostatic pressure (45mm), use gravity to inject (gravity injection) (20 seconds) and use sample, produce the sample volume of 9.2ml.Each experiment is constant 18, and 000V carries out.Collect data at 232nm.By with come diagnostic peak at our prepared in laboratory and the disaccharide that characterizes and the common injection of oligosaccharide reference material.(1.5 * 25cm) go up and measure the size of handling the formed product of gastropod GAG with heparinlyase II, measure (carbazole assay) monitoring with 0.2M sodium chloride eluting and with carbazole at Sephadex G-50 (superfine (superfine)) post by gel permeation chromatography.The disaccharide product that preparation heparinlyase II handles is used for by half preparation type reinforcing YIN-essence ion exchange HPLC analysis and by chromatography desalination on 5-cm * 0.5-m Bio-Gel P-2 post.These results demonstrate complexity and unique composition of the polysaccharide in the Gastropod biological fluid of the technology of the present invention.

Gradient PAGE analyzes

With heparinlyase I, heparinlyase II or heparinlyase III digestion gastropod GAG.Go up enforcement gradient PAGE at polyacrylamide linear gradient separation gel (14 * 16cm, the acrylamide that 12-22% is total).Operation as untreated Limax GAG (swimming lane a), the Limax GAG (swimming lane d) that handles of heparinlyase I the Limax GAG (swimming lane b), the heparinlyase II that handle the Limax GAG (swimming lane c), the heparinlyase III that handle and from the sample of the Heparin Oligosaccharides reference material ladder (swimming lane e) of pulmonis Bovis seu Bubali heparin preparation.Make oligosaccharide as seen by alcian blue dyeing.The result can be referring to Fig. 1, and this figure shows that the Gastropod biological fluid of the technology of the present invention has the complexity composition of Dan Baijutang.

Helix Aspersa M ü ller is carried out histochemical stain to measure glycoprotein concentration

In order to measure the protein composition of Helix Aspersa M ü ller, Helix Aspersa M ü ller slice is complied with in the Kano fix in (family name) fixative (60% ethanol, 30% chloroform, 10% acetic acid) and 4% paraformaldehyde and be embedded among the paraplast.By the improved Mallory method of Ignesti (Carazzi hemalum, 1% acid fuchsin, 1% phosphomolybdic acid, Mallory ' s solution) stained.Use the histochemical method of the following description protein, glycoprotein and acidic protein polysaccharide.For protein, use bromophenol blue method, 1,2,3-indantrione monohydrate-schiff's reaction (ninhydrin-Schiff), toluene-sodium-sulfonchloramide-schiff's reaction (chloramines T-Schiff), peroxyformic acid-schiff's reaction (performic acid-Schiff), Morel and Sisley, Millon and Adams reaction, diazo-reaction and sakaguchi reaction.For glycoprotein, use alcian blue-periodic acid schiff (alcian blue-Periodic acid Schiff, AB-PAS) reaction and 1,1-Dimethyl-3,5-diketocyclohexane-PAS reaction according to McManus.In order to test the acidic protein polysaccharide, use AB (alcian blue) (pH 1) and AB (pH 2.5).Use AB-PAS (pH 1 and 2.5) to distinguish glycoprotein and acidic protein polysaccharide.In order to distinguish acidic protein polysaccharide, use AB-C.E.C. (CEC) dyeing, 0.05%AB, with 0.5-1M MgCl in acetate buffer (pH 5.8) with different acidity ₂The classification molar concentration suppress close A Lixin (alcianophilia); A little less than methylate-AB (pH 2.5); (pH 2.5) methylate by force-AB; A little less than methylate-saponification-AB (pH 2.5); (pH 2.5) methylate by force-saponification-AB.In 0.1M acetate buffer (pH 2.5),, and handle with AB (alcian blue) (pH 2.5) in 60 ℃ of acid hydrolysis 4 hours, and not saponification.The method of enzymic digestion: PAS-amylase; AB-neuraminidase (pH 2.5, from Clostridiumperfrigens), saponification and not saponification; Testicular hyaluronidase.In order to measure specific sugared residue, use biotinylated agglutinin and avidin-biotin enzymatic peroxidation (avidin-biotinperoxidase method) (ABC).Employed biotinylated agglutinin, their origin, specificity and inhibition sugar (inhibiting sugars) have been studied.In order to disclose nNOS, at 4 ℃, in the chamber of humidity with 1: 200 the dilution the anti-human brain nNOS of multi-clone rabbit antibody (nNOS type i antibody; Code 606-259-1550) tissue slice is incubated overnight.Then, should cut into slices in PBS (phosphate buffered saline (PBS) (phosphate buffer saline)) washing and with goat anti-rabbit igg-peroxide enzyme conjugates (1: 100) incubation 2 hours.By cutting into slices 0.015%3,3 '-diaminobenzidine is containing 0.005%H ₂O ₂0.01M Tris (Tris) buffer (pH 7.6) in solution in room temperature incubation 5-12 minute, make peroxidase activity as seen.Negative control comprises that omitting one resists (the primaryantibody) or replace it with the non-immunity rabbit anteserum.

In Helix Apsera M ü ller Limax mantle, skin is made up of the quite lax connective tissue that muscle fiber crosses.At the edge of mantle, there are four dark pleats, locate outside simple epithelium at this and only form, and also have goblet cell in the depths of pleat by the cuboid cell of non-cilium.Myxocyte is considered to contain glycoprotein and the Dan Baijutang with acidic-group, referring to for example, and Fig. 2-2.

Histochemical method has disclosed " protein " cell among the epithelial cell in mantle and foot.The diazo-reaction of carrying out with fast blue in alkaline medium shows that " phenol " cell exists only in the mantle.The PAS positive cell shows that glycoprotein exists only in the mantle.Dyeing is not present in the section of handling with beta amylase, shows " glycogen " epithelial cell that exists in the connective tissue below with the myxocyte coexistence.

And, the reaction that shows acid glycosamine glycan has disclosed five types myxocyte in the mantle: the cell (referring to for example, Fig. 2-3) that only has glycoprotein, has Sulfated acidic protein polysaccharide, has carboxylic Dan Baijutang and have glycoprotein and have carboxylic Dan Baijutang.Some myxocytes of foot epithelium contain the acid Dan Baijutang of carboxyl (referring to for example, Fig. 2-4) and other glycoprotein (referring to for example, Fig. 2-5).In the crust of mantle and foot, do not find sialic acid and hyaluronic acid.Use ConA, PHA-L, PNA, WPA to show with SBA agglutinin (it is all in conjunction with different sugar moieties); the last Intradermal of mantle and foot and the secretions of intradermal myxocyte for δ-D-glucose and δ-D-mannose (referring to for example; Fig. 2-6), oligosaccharide is (referring to for example; Fig. 2-7), δ-D-gal and (1-3)-D-Gal-Nac is (referring to for example; Fig. 2-8), δ-L-fucose is (referring to for example; Fig. 2-9) and N-acetyl group-D-galactosamine or D-galactose (referring to for example, Fig. 2-10) be male.In the superficial epithelium of mantle, seldom neuroendocrine cell is male for WGA (referring to for example, Fig. 2-1).In the mantle epithelium, found the male non-myxocyte of nNOS-(referring to for example, Fig. 2-2).

Embodiment 3: Gastropod biological fluid is induced the fibroblast in-vitro multiplication and is adjusted to the reorganization of fibrocyte cytoskeleton.

Whether relevant for the regeneration properties of studying Gastropod biological fluid (being labeled as SJ, " Limax juice (snail juice) ") with enhanced cell propagation, measured the influence of biofluid to the fibroblast in-vitro multiplication.

In order to measure fibroblast proliferation, every hole 25,000 fell fibroblasts are applied in the 12-orifice plate and with the Helix M ü ller biofluid (SJ) and 0.1mM citrate (pH 5.0) of 50 μ l that indicate or 100 μ l, 100U/ml LMW heparin or 1 μ M, six sulphuric acid mesoinositols (inositolhexasulfate) are handled a week together.Monolayer is washed with PBS, fixing in 10% formalin, and use the distilled water rinsing.Then cell was dyeed 30 minutes with 0.1% crystal violet (Sigma), fully rinsing and dry.With 2.0ml 10% acetic acid extraction cell-association dyestuff.With aliquot H ₂O carries out dilution in 1: 4, is transferred to 96-hole microwell plate, and measures optical density at 590nm.Value is normalized to untreated cell the 0th day optical density.In experiment, every bit is measured in triplicate; Each growth curve is implemented at least twice.Be shown in Fig. 3 with the growth curve of Limax serosity under citrate existence and non-existent situation.What is interesting is, improved cell proliferation from the biofluid of Helix Aspersa M ü ller, by adding citrate cell proliferation is further strengthened, described citrate has proved induces fibroblast proliferation.This result has obtained further reinforcement, because the biofluid of the technology of the present invention has improved the cell survival when using the UVA light radiation, and this hint, the beneficial effect of viewed biofluid is because the compound action of antioxidation and propagation.

Because extracellular matrix (ECM) assembling is the critical events in the tissue regeneration, so measured the effect of the Gastropod biological fluid of Helix Aspersa M ü ller.On the glass cover slide, use Ham ' s F-12 culture media supplemented agent (medium supplements) to cultivate 24 hours in the CHO-K1 cell with penicillin/streptomycin and 10% hyclone.Then, under existence of 5 μ g/ml people fibronectins or non-existent situation, handle cell, and after 24 hours, assemble by indirect immunofluorescence monitoring fibronectin with 50 or 100 μ g/ml Helix Aspersa M ü ller biofluids (SJ).Then cell is fixed 10 minutes in cold methanol.Fibronectin with anti--80kDa polyclonal antibody dyeing, is followed with the bonded anti-rabbit antibody incubation of Alexa488-.Sample is installed among the Mowiol, and with the Leica DMR photomicroscope inspection with 63x and 100x immersion objective lens.Use Leica Qfish software processes image, the results are shown in Fig. 4.Compare with control cells, the Gastropod biological fluid of Helix Aspersa M ü ller is induced the fibronectin assembling.What is interesting is that as if biofluid increased the fibronectin secretion, because it has increased the fibronectin deposition of cell under the situation that does not have the external source fibronectin.For the increase that fibronectin is described is not because the fibronectin that exists in the secretions uses one group of anti--fibronectin antibody to analyze biofluid by ELISA and Western blotting.Find that biofluid does not contain the fibronectin reactivity, as passing through among Western blotting or the ELISA with as shown in the equal anergy of any antibody.These experiment showed, that the effect of viewed Gastropod biological fluid in fibronectin assembling and secretion is because the effect of its pair cell, rather than because the direct deposition of contained ECM in the extract.Such presentation of results the component of uniqueness of Gastropod biological fluid of the technology of the present invention form and character.

In order to be determined at the change of handling back fibroblast form with Helix Aspersa M ü ller Gastropod biological fluid, the fell fibroblast of healthy volunteer's donor was cultivated 24 hours under existence of 100 μ g/ml biofluids or non-existent situation.With cell with 4% formaldehyde fixed among the PBS and use 0.1%Triton X-100 to change thoroughly.For F-actin dyeing, in room temperature with 1 μ g/ml Alexa488-bonded virotoxins (Invitrogen) incubation of coverslip in PBS 1 hour.Sample is installed as mentioned above and checked.At least 200 cell/conditions of three independent experiments have been checked.The results are shown in Fig. 8.By using alcian blue technology (by the arrow in (A) among Fig. 5 and the rod (B) illustrate) to make horizontal Dan Baijutang (transverse proteoglycans) as seen.Also labelling secreting type fibroblast (f) and microenv (me) (scale rod 0.2mm).Described biofluid influences the fibroblastic form of fell, thereby induces actin reorganization, bunchy (bundling) and microfilament to arrange, and causes cell elongation.This presentation of results some peculiar properties of Gastropod biological fluid of the technology of the present invention.

Embodiment 5: the antioxidant effect of Gastropod biological fluid

Provide the ability of antioxidant properties in order to test the Gastropod biological fluid that derives from Helix Aspersa M ü ller, use can be from Sigma Chemical Co, the glutathione-S-transferase that St.Louis MO is purchased (GST) colorimetric determination kit measurement glutathione S-transferase (GST, the molecule that in cell detoxifcation, plays a crucial role) active increase, and use superoxide dismutase (SOD) test kit that can be purchased from Calbiochem Co (San Diego CA).In the 1mg/mlSCA suspension, measure GST and SOD (antioxidase) activity according to the rules of test kit manufacturer.In test kit, provide positive and negative control thing and according to the use of being indicated.The ABTS (2 that use can be purchased from Vector Labs (Burlingame CA), 2 '-azine group-two (3-ethyl benzo thiazole phenanthrolines-6-sulfonic acid, 2,2 '-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid))) the antioxidant test kit tests Gastropod biological fluid (" SJ ") (100 μ g/ml), Quercetin (2.5 μ M) and Trolox C (10 μ M) or independent carrier with 300s.Before adding described reagent and afterwards. in of the absorption of different point in time measurement, this experiment is carried out in triplicate at 743nm.Fig. 6 shows the representative result of this experiment.This digital proof, the technology of the present invention Gastropod biological fluid has antioxidation and radical scavenging activity concurrently.

Embodiment 6: cream medicine is treated the preparation of the embodiment of cosmetic formulations

Prepare the product of 1000g altogether by adding listed composition, prepare following preparation.Product is fully mixed in the pharmacy blender.

Preparation I:

Olive oil acid polyethylene glycol (4) ester (about 8g is to about 10g)

Anhydrosorbitol olive oil acid esters (from olive oil) (about 8g is to about 10g)

Squalane (from olive oil) (about 8g is to about 10g)

Gastropod biological fluid filtrate (about 100ml is to about 150ml)

Hyaluronic acid (about 8g is to about 12g)

Glycerol (about 110g is to about 130g)

Ben Yangyichun ﹠amp; Ethylhexyl glycerol (about 9g is to about 10g)

Corundum oxide (corundum oxide) (about 4g is to about 6g)

Distilled water (about 500ml) adds enough water so that product weight reaches about 1000g.

Preparation 2:

Olive oil acid polyethylene glycol (4) ester (about 10g)

Anhydrosorbitol olive oil acid esters (from olive oil) (about 10g)

Squalane (from olive oil) (about 10g)

Gastropod biological fluid (about 150ml)

Hyaluronic acid (about 10g)

Glycerol (about 120g)

Ben Yangyichun ﹠amp; Ethylhexyl glycerol (about 10g)

Corundum oxide (about 5g) and

Preparation 3:

Olive oil acid polyethylene glycol (4) ester (about 10g)

Anhydrosorbitol olive oil acid esters (from olive oil) (about 10g)

Squalane (from olive oil) (about 10g)

Gastropod biological fluid (about 100ml)

Hyaluronic acid (about 10g)

Glycerol (about 120g)

Ben Yangyichun ﹠amp; Ethylhexyl glycerol (about 10g)

Corundum oxide (about 5g) and

Preparation 4:

Stearic acid (about 65 to about 70g)

Glyceryl monostearate (about 4 to about 5g)

White oil (about 45 to about 50ml)

Silicone oil (about 12ml is to about 15ml)

Octadecanol (about 4g is to about 5g)

Glycerol (about 55ml is to about 60ml)

Gastropod biological fluid (about 100ml to 150ml)

Potassium hydroxide (about 2.2g is to about 2.5g)

Essence (about 4.5ml is to about 5ml)

Ben Yangyichun ﹠amp; Ethylhexyl glycerol (about 9g is to about 11g)

Distilled water (about 600ml) adds enough water so that product weight reaches about 1000g.

Preparation 5:

Stearic acid (about 70g)

Glyceryl monostearate (about 5g)

White oil (about 50ml)

Silicone oil (about 15ml)

Octadecanol (about 5g)

Glycerol (about 60ml)

Gastropod biological fluid (about 100ml)

Potassium hydroxide (about 2.5g)

Essence (5ml)

Ben Yangyichun ﹠amp; Ethylhexyl glycerol (about 10g)

Distilled water (about 625ml) adds enough water so that product weight reaches about 1000g.

Preparation 6:

Stearic acid (about 70g)

Glyceryl monostearate (about 5g)

White oil (about 50ml)

Silicone oil (about 15ml)

Octadecanol (about 5g)

Glycerol (about 60ml)

Limax serosity (about 150ml)

Potassium hydroxide (about 2.5g)

Essence (about 5ml)

Ben Yangyichun ﹠amp; Ethylhexyl glycerol (about 10g)

Preparation 7:

Rosehips seed oil (about 8 to about 10ml)

Gastropod biological fluid filtrate (about 100 to about 150ml)

Olive oil emulsifying agent (about 8 to about 10g)

Sargassum extract (about 18g is to about 22g)

Hyaluronic acid (about 8g is to about 10g)

Chamomile (about 28g is to about 32g)

Sugar isomerate (about 38g is to about 42g),

Radix Glycyrrhizae (Glycyrrhiza inflata) extract (about 22g is to about 25g)

Antiseptic (about 18g is to about 22g): the following plants extract with trace prepares: leaf of Herba Origani extract, Thymi Serpylli Herba extract, Cortex Cinnamomi, leaf of Herba Rosmarini Officinalis extract, lavender flower extract, Fericarpium Citri Limoniae extract, Folium Menthae extract, goldenseal root extract and Olive leaf P.E

Preparation 8:

Rosehips seed oil (about 10ml)

Gastropod biological fluid filtrate (about 100ml)

Olive oil emulsifying agent (about 10g)

Sargassum extract (about 20g)

Hyaluronic acid (about 10g)

Chamomile (about 30g)

Sugar isomerate (about 40g),

Radix Glycyrrhizae (Glycyrrhiza inflata) extract (about 25g)

Antiseptic (about 20g): the following plants extract with trace prepares: Adeps Bovis seu Bubali (Adeps Bovis seu Bubali) leaf extract, Herba thymi vulgaris (Herba thymi vulgaris) extract, Cortex Cinnamomi (Cortex Cinnamomi) peel extract, Herba Rosmarini Officinalis (Herba Rosmarini Officinalis) leaf extract, lavandula angustifolia (lavandula angustifolia) flower extract, Fructus Citri Limoniae (Fructus Citri Limoniae) peel extract, Herba Menthae (Herba Menthae) leaf extract, goldenseal (goldenseal) root extract and Fructus Canarii albi (Fructus Canarii albi) leaf extract

Distilled water (600ml) adds enough water so that product weight reaches about 1000g.

With standard mixer compositions is mixed to evenly.

Embodiment 7: can be added into the preparation of Gastropod biological fluid with the excipient of preparation medication cosmetics

The preparation of excipient that is preferred for containing the technology of the present invention therapeutic composition of the technology of the present invention Gastropod biological fluid is carried out by the following method: with standard method fusion spermol (15g), the Polyethylene Glycol mixture [PEG400 (49.%), 1500 (1.0%) and 4000 (49.5%)] that adds 50g then is to form first mixture.When reaching fusing point, this mixture is transferred to commercial pharmacy laboratory (commercial pharmaceutical laboratory).Second mixture prepares by the following method: fusion 5g glyceryl monostearate and interpolation are heated to distilled water or the preferred physiological serum (physiological serum) of 80 ℃ 12.6g (12.6ml) in advance.When the temperature of the temperature of second mixture and first mixture is identical, second mixture is added into first mixture.Continue on one side to stir, on one side 7g calcium carbonate and 0.4g zinc oxide (the two all is the micropowder form) and 10ml paraffin oil are added into mixture.

Embodiment 8: the preparation of therapy paste frost

About altogether Gastropod biological fluid of 25 to about 30ml (or lyophilizing fluid of about 25 to about 30g) is mixed in about 30 ℃ to about 40 ℃ in blender with about 500 to about 600g above-mentioned excipient.Obtain about altogether 500 to about 700g cream frost.

Embodiment 9: the preparation of nutrition facial cream

Prepare the cream frost by the following method: in above-mentioned excipient, mix 10ml Gastropod biological fluid, 0.2g pantothenic acid and 0.2g heparin, altogether 200 grams.This cream frost is in harmonious proportion skin and rejuvenates.

Embodiment 10: the preparation of nutrition hands frost

Prepare the cream frost by the following method: mix 5g Gastropod biological fluid, 4cc. glycerol and 0.5g lanoline, about altogether 100 grams.The cream frost of gained is very smooth and sprawl good.It can be used by day or at night, with regeneration, more living and glabrous skin.

Embodiment 11: the preparation of purification and wetting liquid

This embodiment has described and has been used for dryness, normal or the purification of oily skin and the preparation of wetting liquid.For the purposes on normal and oily skin, prepare described liquid by mixing 200g excipient, 20cc. Gastropod biological fluid, 0.3g aluminium potassium sulfate and 10cc. rose water.For the purposes on dry skin, prepare liquid as mentioned above, different is to add 2cc. mineral oil, for example, paraffin oil.Can randomly add coloring agent.The cleaning liquid of gained and glabrous skin, and the transparency of the raising colour of skin.It also is used as the foundation cream of cosmetics (for example, powder).At last, its protection skin is avoided the illeffects of cosmetic powder (this cosmetic powder is 1 mineral composition by hardness number on the Mohr scale).

Instant moistening

In order to determine the wetting effect of the Gastropod biological fluid in the cream frost, use Corneometry to measure the moistening kinetics of the cream frost that contains 2%, 5% or 10% the technology of the present invention Gastropod biological fluid.Use separately with 2%, 5% and 10% Gastropod biological fluid with the product of the form of aqueous gel preparation after the measure of time humidity level of 0 minute (T0), 30 minutes, 2 hours and 4 hours.The results are shown in Fig. 7.The amount of application of preparation with maximum dose level Gastropod biological fluid (10%) is enough high, with the contained water of retaining part temporarily in cosmetic formulations.After 30 minutes, write down the highest 30.8% moistening increase.(2h 4h) discloses reading afterwards, and the water of percutaneous gathers, and does not dissipate in a large number.As for medium and low concentration (5% and 2%), temporal effect in the time of 30 minutes is not so remarkable, but pass in time and still kept more moisture, thereby the importance that the water of having strengthened percutaneous keeps, the water of described percutaneous keeps being considered to making its do not dissipate (kinetic factor) because the film forming effect of Gastropod biological fluid is gathered the water of percutaneous.

The long-acting wetting effect that contains the cream frost of Gastropod biological fluid

In order to estimate the long-acting wetting effect of the cream frost that contains Gastropod biological fluid of the present invention, the moistening dynamics research is extended to 21 days.The research participant is to their skin application formulations (10% Gastropod biological fluid or placebo (Placebo)), twice of every day.Measured each research participant's humidity level at the 22nd day, and the result is average, as shown in Figure 8.Compare at (D0/T0) initial value before that begins one's study with each volunteer, Gastropod biological fluid (Limax serosity) shows higher (+12.5%) wet water dawn of the 22nd day on processing region.These results show, compare with placebo, and this product of repetitive administration has cumulative wetting effect.

Embodiment 12: the preparation of mediation and clean liquid

Prepare liquid by the following method: mix 10cc. Gastropod biological fluid, 100cc. physiological serum, 0.3g aluminium potassium sulfate, 0.5g boric acid and 0.1g copper sulfate, 100g altogether.Recommendation is used in this liquid on the tender and lovely skin or exists on the skin of acne.It produces very frank sensation and demulcent quiet sensation.

Embodiment 13: the preparation of anti-wrinkle cream

The anti-wrinkle cream that use every day prepares by the following method: mix 5cc. Gastropod biological fluid, 7g lanoline and 1.56g aluminium potassium sulfate, and be added into basic excipient or physiological serum, 250g altogether.One week, maximum expendable condensed cream frosts prepared as mentioned above, different 15g active component, 15g lanoline and the 2g aluminium potassium sulfates of being to use.This cream frost is easy to use and only be used on the zone that has wrinkle.Active component is effective aspect the minimizing wrinkle, and other component produces astriction.

Implement to be used to measure the skin surface morphological analysis (Skin topographyanalysis) of anti-wrinkle cream effect, on certain facial zone of crossing with anti-wrinkle cream or placebo treatment of selected healthy women volunteer, obtained the silicon marking (silicon imprints).Handle 0,15 and 30 day (twice of every day) afterwards, obtaining the silicon marking.In order to realize the numeral of skin surface, used capacitance apparatus (capacitive device).By going up this equipment of applying light, obtain image in real time at the ROI of skin (region of interest).This equipment (fingerprint that initial design is used for biometric applications obtains and discerns) depends on effective capacitance pixel induction technology (active capacitive pixel sensing technology), can obtain the detail image of skin with the resolution of 50 μ m/ pixels.The array of induction apparatus is made up of 256 * 360 pixels, and is 12.8 * 18.0mm.The surface of each pixel is made up of two adjacent metal plates.When making skin alive (live skin) near sensor board, skin disturbs the field line between the two boards, and it is minimum that feedback capacity is reduced to.On the contrary, when with skin when sensor surface is removed, feedback capacity increases to maximum.Therefore,, and then, regulate the gray value of each pixel, represent the gray value of the output image of three-dimensional skin sensor to obtain the diagram of skin surface according to the distance between sensor board and the skin surface three-dimensional curve according to survey electric capacity.

The high-definition picture acquisition details that provide from capacitance apparatus are easy.Yet many factors can influence the gray value that sensor device provides: (1) puts on the pressure of sensor surface during obtaining; (2) the suction situation of skin influences the gray value of gained capacitance image partly; (3) output of capacitance apparatus response changes according to pixel displacement (pixel displacements), because pick off demonstrates different behaviors on the top of cell array; (4) electric capacity background image (image that obtains under the situation of no any material on the device senses device zone, hereinafter referred is a background) tends to be biased to higher gray value; (5) noise changes the final gray value of capacitance image.In the face of these problems, crucial is that the skin surface morphosis that detects from capacitance image is inferred real real metric system measured value (real absolute metricmeasures).

The first of experiment is the plan range of measuring between the configuration of surface structure of good identification.We will concentrate on wrinkle (by the high gray value identification with respect to surrounding skin), and it seems more black by contrast in capacitance image.At first, implement pre-treatment step, to reduce above-mentioned misleading factor.Then, we extract the profile of wrinkle, the profile of wrinkle are transformed by using normal cubic spline (natural cubic spline) with analytical form.Then, we measure the distance (hereinafter, abbreviating IWD as) between wrinkle, that is, in the distance of micron, described local minimum is extracted and belonged to adjacent wrinkle from the 2D profile between through the axle of the local minimum of two orders.The second portion of experiment is devoted to verify these measured values by these measured values are compared with the measured value in the identical body position zone that obtains by profile determination and analysis (profilometric analysis).Analyzed organosilicon skin copy with the optical profile analyzer of being furnished with 20 * enlarging objective (20 * magnification objective) and .5 * scale factor reduction lens (.5 * scale factorreducer lens), therefore obtain 10 * overall gain.This system (based on white light interferometric method) provides the accurate surface data degree of depth (accurate surface data depths) of the finite region of copy, and the visual field is 0.62 * 0.47mm ², image resolution ratio is 640 * 480 pixels.

Surpassing test condensed cream frost on 200 healthy women volunteers.This group is classified, and tested 30 days with above-mentioned anti-wrinkle cream or placebo.After each was used, anti-wrinkle effect continued an about week.Wrinkle is many more and big more, and the visual effects of this processing of analyzing gained by follow-up observation is just obvious more.Shown in diagrammatic representative result among Fig. 9, (Fig. 9 B and C) compares with the skin of crossing with placebo treatment, and the degree of depth of the wrinkle of the skin of handling with anti-wrinkle cream (Fig. 9 E and F) reduced after 15 and 30 days.The observed result that wrinkle depth obviously reduces after handling 30 days has confirmed to contain the crease-resistant benefit of the cream frost of the technology of the present invention Gastropod biological fluid.

Embodiment 14: the preparation of sunscreen cream

By mixing 250g material (having 5ml Gastropod biological fluid filtrate) preparation sunscreen cream altogether.Sun-proof solarization black frost prepares as mentioned above, and different is to add 1g metal iodide and 0.3g vitamin C.As preventive, it is just enough to use the skim sunscreen before being exposed to daylight.If tanned severely, can use the skim sunscreen cream in per six hours.

In order to test sunscreen, use sunscreen cream to handle 306 fair-complexioned (fair-complexioned) experimenters.Product prophylactically is used for 6% experimenter, is used for to therapeutic 94% experimenter.Sunscreen is used to handle experimenter's (this experimenter demonstrates foaming (blistering) (blister (phlytena))) who is tanned severely at the swimming pool with highly chlorinated water and the people who is tanned severely at the seabeach.In each case, described cream frost after using a few second internal diabetes except the pain and the sense of burning, blister disappeared in 5 to 6 hours; Sunburn disappeared in 24 hours.

Ultraviolet radiation causes such as sunburn, photosensitivity reaction or immunosuppressant adverse effect.The purpose of this research is to estimate Pharmacom by measuring erythema index and the human intradermal high molecular antioxidant of evaluation and its metabolite ^TMThe light protection result of Younger Sunscreen Cream.Use the sign of these materials as oxidation effectiveness.Eight health female experimenters in this research, have been recruited.Two abdomen area are exposed to daylight simulation radiation, use three kinds of minimum erythema doses, use sunscreen cream for one, another piece is not used sunscreen cream.Use two other zones in contrast, do not use SPF8 for one, another piece is used SPF8.Collect ascorbic acid and its metabolite (hydroascorbic acid, threonic acid THREONIC ACID., oxalic acid and xylose) from human corium by micro-dialysis, and measure by gas chromatography-mass spectrometry.Compare with other three positions, the radiation position of not using sunscreen obviously demonstrates lower corium ascorbic acid concentrations and higher erythema index (P＜0.05).With compare at control site I, the corium concentration of threonic acid THREONIC ACID., oxalic acid and xylose is in position III significantly higher (P＜0.05).Shielded radiation position does not show that erythema forms, and ascorbic acid corium concentration has stability, the metabolite no change.These result's hints, the sunscreen composition of the technology of the present invention can and prevent from as sunscreen skin is caused oxidation and UV infringement.

Now with so fully, clear and simple and clear term description the technology of the present invention, make those skilled in the art can put into practice the present invention.Will be appreciated that, above described the preferred implementation of the technology of the present invention, and can change, and break away from the purport and the scope of the technology of the present invention that the claim of enclosing sets forth it.

Claims

1. method of collecting Gastropod biological fluid said method comprising the steps of:

1) the activated gastropod of thorn is to increase the biofluid secretion;

2) collect described excretory biofluid;

3) centrifugal described secreted fluid is to produce supernatant; With

4) filter described supernatant contains the amount of raising with recovery the filtrate of glycosaminoglycans.

2. the method for claim 1, it also comprises makes jejunitas about 1 day to about 5 days of described gastropod to reduce the step of the toxin in the described excretory biofluid.

3. the method for claim 1, wherein said gastropod is Helix Aspersa M ü ller.

4. the method for claim 1, wherein said gastropod is Helix pomatia.

5. the method for claim 1, wherein said Gastropod biological fluid comprises (1 → 4)-2-acetyl group, 2-deoxidation-α-D-Glucopyranose. (1 → 4)-2-sulfo group-α-L-pyrans idose base alduronic acid.

6. the method for claim 1, the step of the gastropod of the described work of wherein said stimulation is to be selected from following method: make described gastropod be exposed to sonic vibration; Make described gastropod be exposed to low high pressure; Make described gastropod be exposed to hypoxia condition; Make described gastropod be exposed to Spurs; Make described gastropod be exposed to ionizing radiation; Make described gastropod be exposed to physical stimulation; With the described gastropod of reversing.

6. method as claimed in claim 5, the wherein said step that makes described gastropod be exposed to sonic vibration comprise that the ultrasonic radiation that makes described gastropod be exposed to about 20kHz was at least about 10 seconds.

7. method as claimed in claim 5, wherein said make described gastropod be exposed to low highly compressed step comprise make described gastropod be exposed to pressure for about 4psi extremely the altitude chamber of about 7.5psi at least about 10 seconds.

8. method as claimed in claim 7, wherein said gastropod were exposed to described altitude chamber at least about 15 seconds.

9. method as claimed in claim 5, the wherein said step that makes described gastropod be exposed to physical stimulation are included in about 1G centrifugal described gastropod about 2 minutes to about 10 minutes between the power of about 6G.

10. method as claimed in claim 9, wherein said centrifugation step is carried out in about 10 ℃ or higher temperature.

11. method as claimed in claim 9, wherein said centrifugation step is carried out in about 15 ℃ or higher temperature.

12. method as claimed in claim 9, wherein said centrifugation step is carried out in about 20 ℃ or higher temperature.

13. method as claimed in claim 9, the temperature of wherein said centrifugation step between about 20 ℃ to about 35 ℃ carried out.

13. a Gastropod biological fluid, it prepares by the method for claim 1.

14. Gastropod biological fluid as claimed in claim 13, wherein said Gastropod biological fluid contain the described glycosaminoglycans of the amount that effectively makes the mankind or animal skin tissue's rehabilitation.

15. medication cosmetics that promote skin regeneration and rehabilitation, it comprises the filtrate at least about the method preparation of passing through claim 1 of 15 weight %.

16. medication cosmetics as claimed in claim 15, it also comprises delivery system.

17. medication cosmetics as claimed in claim 16, wherein said delivery medium comprise cream frost, gel, solution, oil, suspension, dispersion, ointment, film, paster, cover, pill, suppository, soap, adhering skin glue, powder, its derivant or their combination.

18. a Gastropod biological fluid, it comprises:

About 0.001% to about 0.1% alduronic acid;

About 0.003% to about 0.3% aminohexose;

About 0.001 to about 0.1% acetyl group thing;

About 0.001 to about 0.1% hydrosulphate;

About 0.02% to about 5.0% at least a mucopolysaccharide;

About 0.05% to about 0.5% at least a fibroblast growth factor;

About 0.01% to about 0.1% at least a enzyme;

About 0.01% to about 1.0% hyaluronic acid;

About 0.001% to about 0.1% at least a carrier of oxygen;

About 0.001% to about 0.1% at least a high molecular weight protein;

About 0.0001 to about 0.01% at least a trace element;

About 0.005% to about 0.1% at least a high molecular anti-oxidant compounds;

About 0.005% to about 0.1% at least a low molecular weight compound; With

About 80% to about 98% water.

19. a Gastropod biological fluid, it comprises:

About 0.02% to about 0.03% alduronic acid;

About 0.03% to about 0.05% aminohexose;

About 0.01 to about 0.02% acetyl group thing;

About 0.01 to about 0.02% hydrosulphate;

About 0.28% to about 0.30% at least a mucopolysaccharide;

About 0.1% to about 0.2% at least a fibroblast growth factor;

About 0.05% to about 0.06% at least a enzyme;

About 0.1% to about 0.2% hyaluronic acid;

About 0.01% to about 0.02% at least a carrier of oxygen;

About 0.04% to about 0.05% at least a high molecular weight protein;

About 0.001 to about 0.002% at least a trace element;

About 0.05% to about 0.06% at least a high molecular anti-oxidant compounds;

About 0.05% to about 0.06% at least a low molecular weight compound; With

About 96.0% to about 96.5% water.

20. Gastropod biological fluid as claimed in claim 19, wherein said at least a mucopolysaccharide comprises (1 → 4)-2-acetyl group, 2-deoxidation-α-D-Glucopyranose. (1 → 4)-2-sulfo group-α-L-pyrans idose base alduronic acid.

21. Gastropod biological fluid as claimed in claim 19, wherein said at least a fibroblast growth factor are to be selected from following member: FGF1, FGF2, FGF3, FGF4, FGF5, FGF6, FGF7, FGF8, FGF9, FGF10, FGF11, FGF12, FGF13, FGF14, FGF15, FGF16, FGF17, FGF18, FGF19, FGF20, FGF21, FGF22, FGF23 and their combination.

22. Gastropod biological fluid as claimed in claim 19, wherein said at least a fibroblast growth factor are FGF1, FGF4, FGF7, FGF10 or their combination.

23. Gastropod biological fluid as claimed in claim 19, wherein said at least a enzyme comprises collagenase, gelatinase, Dan Baijutang enzyme, its derivant or their combination.

24. medication cosmetics, its comprise by weight at least about 1% to about 30% contain (1 → 4)-2-acetyl group, the Gastropod biological fluid of 2-deoxidation-α-D-Glucopyranose. (1 → 4)-2-sulfo group-α-L-pyrans idose base alduronic acid.

25. medication cosmetics as claimed in claim 24, it comprises by weight the described Gastropod biological fluid at least about 10% to about 25%.

26. medication cosmetics as claimed in claim 24, wherein said Gastropod biological fluid are the supernatant biofluids.

27. medication cosmetics as claimed in claim 24, wherein said Gastropod biological fluid are (1 → the 4)-2-acetyl group that contains effective dose, the filtrate of 2-deoxidation-α-D-Glucopyranose. (1 → 4)-2-sulfo group-α-L-pyrans idose base alduronic acid.

28. medication cosmetics as claimed in claim 24, it also comprises the Squalene of effective dose.

29. medication cosmetics as claimed in claim 28, wherein said Squalene account for about 0.3% to about 3.0% of described medication cosmetics by weight.

30. medication cosmetics as claimed in claim 29, wherein said Squalene account for about 0.8% to about 1.0% of described medication cosmetics by weight.

31. medication cosmetics as claimed in claim 24, it also comprises the hyaluronic acid of effective dose.

32. medication cosmetics as claimed in claim 31, wherein said hyaluronic acid account for about 0.3% to about 3.0% of described medication cosmetics by weight.

33. medication cosmetics as claimed in claim 31, wherein said hyaluronic acid account for about 0.5% to about 1.2% of described medication cosmetics by weight.

34. a skin care compositions and methods, it comprises:

The gastropod of biologic effective dose secretion fluid and

Can be applied to human or zoodermic delivery medium.

35. skin care compositions and methods as claimed in claim 34, wherein said delivery medium comprise cream frost, gel, solution, oil, suspension, dispersion, ointment, film, paster, cover, pill, suppository, soap, adhering skin glue, powder, its derivant or their combination.

36. skin care compositions and methods as claimed in claim 34, its also comprise about 0.3% to about 3.0% Squalene and about 0.3% to about 3.0% hyaluronic acid.

37. skin care compositions and methods as claimed in claim 32, it also comprises at least a antiseptic, at least a antioxidant, at least a solvent, at least a brightening agent and at least a abrasivus.

38. skin care compositions and methods as claimed in claim 37, wherein said at least a antiseptic comprises phenoxyethanol, ethylhexyl glycerol or their combination.

39. skin care compositions and methods as claimed in claim 37, wherein said at least a antiseptic account for about 0.1% to about 4.0% of described skin care compositions and methods by weight.

40. skin care compositions and methods as claimed in claim 37, wherein said antioxidant are to be selected from following member: olive oil derivant, oleic acid, Palmic acid, uric acid, carotenoid, vitamin Q, thioctic acid, vitamin C, vitamin E, phenolic compound, resveratrol, beta-carotene, selenium, high molecular anti-oxidant compounds, low molecular mass antioxidants chemical compound, its derivant and their combination.

41. skin care compositions and methods as claimed in claim 37, wherein said antioxidant account for about 0.1% to about 10% of described skin care compositions and methods gross weight by weight.

42. skin care compositions and methods as claimed in claim 37, wherein said antioxidant account for about 2.0% to about 3.0% of described skin care compositions and methods gross weight by weight.

43. skin care compositions and methods as claimed in claim 37, wherein said solvent are to be selected from following member: glycerol, water or their combination.

44. skin care compositions and methods as claimed in claim 43, wherein said solvent comprise about 60.0% to about 70.0% water and about 10.0% to about 20.0% glycerol, based on the gross weight of described skin care compositions and methods.

45. skin care compositions and methods as claimed in claim 37, wherein said solvent account for about 50.0% to about 90.0% of described skin care compositions and methods gross weight.

46. skin care compositions and methods as claimed in claim 37, wherein said abrasivus are the corundum oxides.

47. skin care compositions and methods as claimed in claim 37, wherein said abrasivus account for about 0.001% to about 0.005% of described skin care compositions and methods gross weight.

48. skin care compositions and methods as claimed in claim 34, it also comprises the olive oil derivant.

49. skin care compositions and methods as claimed in claim 48, wherein said olive oil derivant is selected from: anhydrosorbitol olive oil acid esters, olive oil acid polyethylene glycol (4) ester, Squalene, its derivant and their combination.

50. skin care compositions and methods as claimed in claim 48, wherein said olive oil derivant account for about 0.5% to about 5.0% of described skin care compositions and methods gross weight.

51. skin care compositions and methods as claimed in claim 34, it also comprises about by weight 2.0% to about 3.0% olive oil derivant, about 10% to about 20% described Gastropod biological fluid, about 0.5% to about 2.0% hyaluronic acid, about 10% to about 15% glycerol, about 0.5% to 1.5% the antiseptic that contains phenoxyethanol and ethylhexyl glycerol and about by weight 0.1% to about 1.0% corundum oxide by weight by weight by weight by weight, based on the gross weight of described skin care compositions and methods.

52. a compositions, it comprises:

About 10.0% to about 25.0% gastropod secretion fluid;

About 0.5% to about 10.0% stearic acid;

About 0.1% to about 1.0% glyceryl monostearate;

About 1.0% to about 10.0% at least a oil;

About 0.1% to about 1.0% octadecanol;

About 1.0% to about 10.0% glycerol;

About 0.02% to about 2.0% potassium hydroxide; With

About 0.2% to about 2.0% antiseptic.

53. compositions as claimed in claim 52, wherein said at least a oil comprises white oil, silicone oil or their combination.

54. compositions as claimed in claim 53, wherein said at least a oil comprise about 1% to about 6% white oil and about 1% to about 2% silicone oil.

55. compositions as claimed in claim 52, it also comprises about 0.1% to about 1.0% essence.

56. a preparation, it comprises:

About 10% to about 20% gastropod secretion fluid;

About 0.2% to about 2.0% Rosehips seed oil;

About 0.2% to about 2.0% olive oil emulsifying agent;

About 0.5% to about 5.0% Sargassum extract;

About 0.1% to about 1.0% hyaluronic acid;

About 0.4% to about 4.0% chamomile extract;

About 0.5% to about 5.0% sugared isomerate;

About 0.3% to about 3.0% Radix Glycyrrhizae extract; With

About 0.3% to about 3.0% antiseptic.

57. preparation as claimed in claim 56, it also comprises about 60% to about 65% water.

58. preparation as claimed in claim 56, wherein said antiseptic comprises one or more plant extracts.

59. preparation as claimed in claim 58, wherein said plant extract are to be selected from following member: leaf of Herba Origani extract, Thymi Serpylli Herba extract, Cortex Cinnamomi, leaf of Herba Rosmarini Officinalis extract, lavender flower extract, Fericarpium Citri Limoniae extract, Folium Menthae extract, goldenseal root extract, Olive leaf P.E, its derivant and their combination.