CN101790957A - Breeding method for maize inbred line - Google Patents
Breeding method for maize inbred line Download PDFInfo
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- CN101790957A CN101790957A CN201010129595A CN201010129595A CN101790957A CN 101790957 A CN101790957 A CN 101790957A CN 201010129595 A CN201010129595 A CN 201010129595A CN 201010129595 A CN201010129595 A CN 201010129595A CN 101790957 A CN101790957 A CN 101790957A
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Abstract
The invention relates to a preparation method for maize hybrids, belonging to the technical field of agricultural new species breeding. By taking MW94 line 449 as a female parent and ETO germ plasm as a male parent, a single hybrid is obtained through hybridization. By taking the single hybrid as a female parent and Luda red cob 340 as a male parent, a triple hybrid is obtained through hybridization. Superior plants are selected after two generations of continuous inbreeding. MW94 line 449 is used as a male parent for hybridization to obtain a base material. Then six generations of inbreeding are conducted to obtain an inbred line 216. The inbred line 216 of the invention has the advantages that a plurality of kinds of diseases such as gray speck can be resisted, the hereditary character is stable and the combining ability is strong, and is a superior inbred line for the preparation of maize hybrids.
Description
Technical field
The present invention relates to a kind of seeding technique of corn hybrid seed, belong to the technical field of agriculture seed selection novel species.
Background technology
Corn inbred line is the basis of corn hybridization use of advantage, preparing hybrid kind.Corn has corresponding adaptability to environment, if will make the corn hybrid seed of high yield, high-quality, many anti-, wide adaptabilities, selecting the good inbred line of hereditary capacity is crucial work.But inbred line increases because of propagation generation, uses the age to increase, and can occur that vitality weakens, disease resistance reduces, degradation phenomenon under the reproductive output, cause the crossbreed disease resistance that makes poor, yield poorly.Therefore the corn inbred line that the urgent need seed selection makes new advances in the agricultural production satisfies the demand of preparation new hybrid.
Summary of the invention
Technical problem to be solved by this invention provides a kind of selection of corn inbred line 216, and the crossbreed that this method selects has king-rod, anti-multiple diseases, stabilization characteristics of genetics, advantage that general combining ability is high.
Solving the scheme that technical problem of the present invention adopts is: present invention includes the seed selection of parental inbred line, distance isolation, proper seeding, rational close planting, artificial hybridization, bagging is isolated and selfing purification series of steps, and breeding is that to select with China ink white 94 be 449 to be female parent in these measures, is male parent with the ETO germplasm, hybridization obtains a single cross hybrid, be female parent with this single cross hybrid again, the red bone in Luda (red 340) is a male parent, after hybridization obtains a triple hybrid, continuously in 2 generations of selfing, selected excellent strain, be 449 be must a basic material after male parent is hybridized with white 94 choosings of China ink again, 6 generations of selfing again, thus obtain inbred line 216.
Of the present invention be 449 to be female parent with white 94 choosings of China ink, with the ETO germplasm is in the paternal hybrid, answer artificial bagging to isolate before female fringe and the tassel heading, gather the pollen of male parent ETO germplasm when weaving silk loose powder, invest white 94 choosings of maternal China ink and be 449 female taking, be enclosed within on the female fringe with sack again, thereby obtain a single cross hybrid.
In the red bone germplasm of described single cross hybrid and Luda (red 340) hybridization, answer artificial bagging to isolate female fringe and tassel before the heading, the pollen of gathering male parent Luda red bone germplasm pellet 340 when weaving silk loose powder is invested on the filigree of maternal single cross hybrid, puts female fringe with sack again, and results obtain a triple hybrid.
In described triple hybrid selfing, the preceding female tassel of artificial bagging homophyletic of loose powder of weaving silk of earing is invested the homophyletic filigree with homophyletic pollen when weaving silk loose powder, puts female fringe with sack again, selects advantage selfing strain.
In white 94 choosings of described advantage selfing strain and China ink is in 449 hybridization, answers artificial bagging to isolate before the loose powder of weaving silk, and when weaving silk loose powder, invests on the maternal filigree with paternal pollen, puts maternal female fringe with sack again, and results obtain basic material.
In the 6 generations selfing of described basic material, the female tassel of artificial bagging homophyletic before the loose powder of weaving silk is invested the homophyletic filigree with homophyletic pollen when weaving silk loose powder, puts female fringe with sack again.
The measure of taking during hybrid seeding is as follows:
1, distance isolation: the distance isolation that inbred line is produced will be more than 500m.
2, proper seeding: stable when soil temperature, sow during by 10 ℃.
3, rational close planting: calculate every mu of 4000 strains of keeping a full stand of seedings with mixed area.
4,,, pull out the assorted strain, diseased plant, the weak plant that do not meet parent's characteristic feature characteristic based on the impurity elimination in seedling stage in seedling stage, shooting stage, should carry out three impurity eliminations before taking out male loose powder.
The standard of inbred line 216
1, source: with white 94 choosings of China ink is 449 to be female parent, and the ETO germplasm is that paternal hybrid gets a single cross hybrid; Be female parent with this single cross hybrid again, the red bone in Luda (red 340) is for paternal hybrid gets a basic material, the continuous excellent strain of selfing 2 generation seed selection; Again with white 94 choosings of China ink be 449 be male parent this with excellent strain hybridize behind the basic material, 6 generations of selfing and obtaining again.
2, feature: 216 inbred lines are in the 135 days time of infertility of Yunnan mountain of papers, seedling leaf sheath aubergine, and seedling growth is slow, and blade is overlapping smooth, leaf look grey violet, cane purple, plant height 180cm, fringe position 70cm, 20～21 of total leaf numbers, fringe upper blade are 6～7, and the tassel branch is medium, and pollen amount is big, grain husk shell lilac, female fringe bract is short, filigree aubergine, fruit ear tubular, 16 centimetres of spike lengths, tassel row number 16-18 is capable, several 26 of row grain, grain white osculant, seed-producing rate 84%, thousand kernel weight 270 grams.
216 inbred lines are identified naturally, high anti-ear rot, black sick, the leaf blight of silk fringe, middle sense helminthosporium maydis, banded sclerotial blight, rust.
Beneficial effect of the present invention:
The inbred line 216 of seed selection of the present invention has king-rod, large spike, and anti-grey leaf spot disease of maize (the main disease of Maize Production at present), anti-Maize Ear Rot, resistant to lodging, barren-resistant, outstanding features such as general combining ability height.Do with 216 maternal, 86 first generation of hybrid F that form as paternal hybrid
1(No. 13, Bi Dan), its various features characteristic is very good, is subjected to liking of vast farmers deeply, and supply falls short of demand for seed.It mainly shows the output height, the Xing Huangdan 892 that promotes mainly than Yunnan, can single No. 4 etc. kind volume increase more than 25%.The general spike length 24cm of especially big rod, the longest 30cm, tassel row number 14～18 row, several about 50 of row grain, seed-producing rate 86.7%, diseases such as anti-graywall, fringe Maize Ear Rot, barren-resistant, drought resistance is strong, suitability is wide, can plant height above sea level is 800～1800m.
Description of drawings
Fig. 1 is a production of hybrid seeds schematic diagram of the present invention.
Embodiment
Embodiment:
The seeding technique of inbred line 216
The basic skills of the production of hybrid seeds: with white 94 choosings of China ink is 449 to be female parent, and the ETO germplasm is that paternal hybrid gets a single cross hybrid; Be female parent with this single cross hybrid again, the red bone in Luda (red 340) is for paternal hybrid gets a basic material, the continuous excellent strain of selfing 2 generation seed selection; Again with white 94 choosings of China ink be 449 be male parent this with excellent strain hybridize behind the basic material, 6 generations of selfing and obtaining again.
1, choosing ground and isolated area setting: select medium above fertility, the plot of irrigation conditions arranged as seed farm.Safety is isolated, and breeding this inbred line will can not have other corn variety with interior at 500 meters.
2, proper seeding: when soil temperature is stablized by 10 ℃, should in time sow, sow in spring in the area, Yunnan and generally can sow in the last ten-days period in March.
3, rational close planting: calculate general every mu of 4000 strains of keeping a full stand of seedings with mixed area.
4, results management and threshing: when the fruit ear maturation, results airing in time prevents to drench with rain during the bract jaundice.When seed moisture content reduces to 16～18%, can threshing.Assorted fringe, variation fringe be should select before the threshing, rot grain, the grain of damaging by worms removed.
5, every mu of fertilizing amount and fertilizer practice: enrich farmyard manure according to actual conditions, 40 kilograms of every mu of general calcium of usefulness, every mu of usefulness is nitrogenous 15%, contain potassium 15%, phosphorous 15% composite fertilizer makes base manure for 40 kilograms, every mu of six leaf phase impose 15～20 kilograms in urea, wait every mu of fringe latter stage and execute 20 kilograms in urea as fringe granulated fertilizer.
Claims (10)
1. the selection of a corn inbred line 216, comprise the seed selection of parental inbred line, distance isolation, proper seeding, rational close planting, artificial hybridization, bagging is isolated and selfing purification series of steps, it is characterized in that: with white 94 choosings of China ink is 449 to be female parent, with the ETO germplasm is male parent, and hybridization obtains a single cross hybrid, is female parent with this single cross hybrid again, Luda Hong Gudan 340 is a male parent, after hybridization obtained a triple hybrid, continuously in 2 generations of selfing, selected excellent strain, is 449 be must a basic material after male parent is hybridized with white 94 choosings of China ink again, 6 generations of selfing again, thus inbred line 216 obtained.
2. the selection of corn inbred line 216 according to claim 1, it is characterized in that: be 449 to be female parent with white 94 choosings of China ink, with the ETO germplasm is in the paternal hybrid, answer artificial bagging to isolate before female fringe and the tassel heading, gather the pollen of male parent ETO germplasm when weaving silk loose powder, invest white 94 choosings of maternal China ink and be 449 female taking, be enclosed within on the female fringe with sack again, thereby obtain a single cross hybrid.
3. the selection of corn inbred line 216 according to claim 1, it is characterized in that: in red 340 hybridization of the red bone germplasm of single cross hybrid and Luda, answer artificial bagging to isolate female fringe and tassel before the heading, the pollen of gathering male parent Luda red bone germplasm pellet 340 when weaving silk loose powder is invested on the filigree of maternal single cross hybrid, put female fringe with sack again, results obtain a triple hybrid.
4. the selection of corn inbred line 216 according to claim 1, it is characterized in that: in the triple hybrid selfing, the preceding female tassel of artificial bagging homophyletic of loose powder of weaving silk of earing is invested the homophyletic filigree with homophyletic pollen when weaving silk loose powder, put female fringe with sack again, select advantage selfing strain.
5. the selection of corn inbred line 216 according to claim 1, it is characterized in that: in white 94 choosings of advantage selfing strain and China ink is in 449 hybridization, answer artificial bagging to isolate before the loose powder of weaving silk, when weaving silk loose powder, invest on the maternal filigree with paternal pollen, put maternal female fringe with sack again, results obtain basic material.
6. the selection of corn inbred line 216 according to claim 2 is characterized in that: in the 6 generations selfing of basic material, the female tassel of artificial bagging homophyletic before the loose powder of weaving silk is invested the homophyletic filigree with homophyletic pollen when weaving silk loose powder, puts female fringe with sack again.
7. according to the selection of claim 2,3,4,5 or 6 described corn inbred lines 216, it is characterized in that: the distance isolation that selfing is produced will be more than 500m.
8. according to the selection of claim 2,3,4,5 or 6 described corn inbred lines 216, it is characterized in that: proper seeding should be undertaken when soil temperature is stablized by 10 ℃.
9. according to the selection of claim 2,3,4,5 or 6 described corn inbred lines 216, it is characterized in that: rational close planting is to calculate every mu of 4000 strains of keeping a full stand of seedings with mixed area.
10. according to the selection of claim 2,3,4,5 or 6 described corn inbred lines 216, it is characterized in that: should carry out three impurity eliminations in seedling stage, shooting stage, before taking out male loose powder, based on the impurity elimination in seedling stage, pull out the assorted strain, diseased plant, the weak plant that do not meet parent's characteristic feature characteristic.
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| CN201010129595A CN101790957A (en) | 2010-03-23 | 2010-03-23 | Breeding method for maize inbred line |
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| CN201010129595A CN101790957A (en) | 2010-03-23 | 2010-03-23 | Breeding method for maize inbred line |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090322A (en) * | 2010-11-29 | 2011-06-15 | 山东冠丰种业科技有限公司 | Seed selection method for new strain of maize inbred line |
| CN102884977A (en) * | 2012-10-18 | 2013-01-23 | 山西屯玉种业科技股份有限公司 | Breeding method of maize inbred line with high resistance, stable yield and high combining ability |
| CN103828714A (en) * | 2014-03-07 | 2014-06-04 | 毕节市农业科学研究所 | Method for induction and diploidization of different-germplasm haploid of corn in northwest Guizhou |
| CN106106156A (en) * | 2016-06-29 | 2016-11-16 | 无锡南理工科技发展有限公司 | A kind of breeding method of anti-rough dwarf disease Semen Maydis |
| CN106386464A (en) * | 2016-09-26 | 2017-02-15 | 四川鑫万发种子科技有限公司 | Corn parental inbred line breeding method |
| CN107347630A (en) * | 2017-08-21 | 2017-11-17 | 山西省农业科学院谷子研究所 | The selection of corn inbred line 99 751 |
| CN110537463A (en) * | 2019-10-17 | 2019-12-06 | 黑龙江省农业科学院耕作栽培研究所 | Corn cultivation method considering high yield and high quality of corn stigma |
-
2010
- 2010-03-23 CN CN201010129595A patent/CN101790957A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090322A (en) * | 2010-11-29 | 2011-06-15 | 山东冠丰种业科技有限公司 | Seed selection method for new strain of maize inbred line |
| CN102884977A (en) * | 2012-10-18 | 2013-01-23 | 山西屯玉种业科技股份有限公司 | Breeding method of maize inbred line with high resistance, stable yield and high combining ability |
| CN103828714A (en) * | 2014-03-07 | 2014-06-04 | 毕节市农业科学研究所 | Method for induction and diploidization of different-germplasm haploid of corn in northwest Guizhou |
| CN106106156A (en) * | 2016-06-29 | 2016-11-16 | 无锡南理工科技发展有限公司 | A kind of breeding method of anti-rough dwarf disease Semen Maydis |
| CN106386464A (en) * | 2016-09-26 | 2017-02-15 | 四川鑫万发种子科技有限公司 | Corn parental inbred line breeding method |
| CN107347630A (en) * | 2017-08-21 | 2017-11-17 | 山西省农业科学院谷子研究所 | The selection of corn inbred line 99 751 |
| CN110537463A (en) * | 2019-10-17 | 2019-12-06 | 黑龙江省农业科学院耕作栽培研究所 | Corn cultivation method considering high yield and high quality of corn stigma |
| CN110537463B (en) * | 2019-10-17 | 2022-02-11 | 黑龙江省农业科学院耕作栽培研究所 | Corn cultivation method considering high yield and high quality of corn stigma |
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Open date: 20100804 |