CN101775363A - 乙醇生产 - Google Patents
乙醇生产 Download PDFInfo
- Publication number
- CN101775363A CN101775363A CN200910204036A CN200910204036A CN101775363A CN 101775363 A CN101775363 A CN 101775363A CN 200910204036 A CN200910204036 A CN 200910204036A CN 200910204036 A CN200910204036 A CN 200910204036A CN 101775363 A CN101775363 A CN 101775363A
- Authority
- CN
- China
- Prior art keywords
- leu
- ala
- val
- ile
- gly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 141
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 23
- 230000001580 bacterial effect Effects 0.000 claims abstract description 66
- 238000000034 method Methods 0.000 claims abstract description 44
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 36
- 238000000855 fermentation Methods 0.000 claims abstract description 24
- 230000004151 fermentation Effects 0.000 claims abstract description 23
- 230000006801 homologous recombination Effects 0.000 claims abstract description 22
- 238000002744 homologous recombination Methods 0.000 claims abstract description 22
- 230000002779 inactivation Effects 0.000 claims abstract description 15
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 50
- 239000013612 plasmid Substances 0.000 claims description 44
- 241000894006 Bacteria Species 0.000 claims description 40
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 36
- 244000005700 microbiome Species 0.000 claims description 30
- 101150104734 ldh gene Proteins 0.000 claims description 29
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 27
- 238000003780 insertion Methods 0.000 claims description 24
- 230000037431 insertion Effects 0.000 claims description 24
- 229920001184 polypeptide Polymers 0.000 claims description 20
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 20
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 20
- 101150067366 adh gene Proteins 0.000 claims description 19
- 108020004414 DNA Proteins 0.000 claims description 18
- 235000014655 lactic acid Nutrition 0.000 claims description 18
- 239000004310 lactic acid Substances 0.000 claims description 18
- 101150034686 PDC gene Proteins 0.000 claims description 14
- 239000002253 acid Substances 0.000 claims description 14
- 230000008521 reorganization Effects 0.000 claims description 14
- 239000013605 shuttle vector Substances 0.000 claims description 14
- 241000588724 Escherichia coli Species 0.000 claims description 13
- 238000003672 processing method Methods 0.000 claims description 13
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims description 12
- 101710088194 Dehydrogenase Proteins 0.000 claims description 12
- 230000001476 alcoholic effect Effects 0.000 claims description 12
- 102000007698 Alcohol dehydrogenase Human genes 0.000 claims description 11
- 238000012408 PCR amplification Methods 0.000 claims description 11
- 241000588902 Zymomonas mobilis Species 0.000 claims description 11
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 229910052760 oxygen Inorganic materials 0.000 claims description 10
- 239000001301 oxygen Substances 0.000 claims description 10
- 102000008579 Transposases Human genes 0.000 claims description 9
- 108010020764 Transposases Proteins 0.000 claims description 9
- 238000012216 screening Methods 0.000 claims description 9
- 230000008859 change Effects 0.000 claims description 8
- 230000010354 integration Effects 0.000 claims description 8
- 230000000975 bioactive effect Effects 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 6
- 210000002966 serum Anatomy 0.000 claims description 6
- 244000144987 brood Species 0.000 claims description 5
- 241000193390 Parageobacillus thermoglucosidasius Species 0.000 claims description 4
- 230000002950 deficient Effects 0.000 claims description 4
- 238000009396 hybridization Methods 0.000 claims description 4
- 230000004071 biological effect Effects 0.000 claims description 3
- 238000012262 fermentative production Methods 0.000 claims description 3
- 241000193385 Geobacillus stearothermophilus Species 0.000 claims description 2
- 241000193749 Bacillus coagulans Species 0.000 claims 1
- 241001468175 Geobacillus thermodenitrificans Species 0.000 claims 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 claims 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 claims 1
- 241000588901 Zymomonas Species 0.000 claims 1
- 241000193761 [Bacillus] caldolyticus Species 0.000 claims 1
- 241000193758 [Bacillus] caldotenax Species 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 238000010924 continuous production Methods 0.000 claims 1
- 230000002427 irreversible effect Effects 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 230000014509 gene expression Effects 0.000 abstract description 9
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 33
- 102220023257 rs387907546 Human genes 0.000 description 20
- 102220369447 c.1352G>A Human genes 0.000 description 16
- 229940107700 pyruvic acid Drugs 0.000 description 16
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 14
- 239000000047 product Substances 0.000 description 13
- 125000003275 alpha amino acid group Chemical group 0.000 description 11
- 230000002829 reductive effect Effects 0.000 description 9
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 8
- 238000013459 approach Methods 0.000 description 7
- 150000001720 carbohydrates Chemical class 0.000 description 7
- 235000014633 carbohydrates Nutrition 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 108091008146 restriction endonucleases Proteins 0.000 description 7
- 102220023258 rs387907548 Human genes 0.000 description 7
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 6
- 239000013611 chromosomal DNA Substances 0.000 description 6
- 230000004060 metabolic process Effects 0.000 description 6
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 6
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 102220369445 c.668T>C Human genes 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 230000035772 mutation Effects 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 108010081577 aldehyde dehydrogenase (NAD(P)+) Proteins 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 230000002503 metabolic effect Effects 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- 150000002972 pentoses Chemical class 0.000 description 4
- 230000002441 reversible effect Effects 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 238000013016 damping Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 108010008221 formate C-acetyltransferase Proteins 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 102000057593 human F8 Human genes 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- XUWPJKDMEZSVTP-LTYMHZPRSA-N kalafungina Chemical compound O=C1C2=C(O)C=CC=C2C(=O)C2=C1[C@@H](C)O[C@H]1[C@@H]2OC(=O)C1 XUWPJKDMEZSVTP-LTYMHZPRSA-N 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 229940047431 recombinate Drugs 0.000 description 3
- 102220004457 rs11567847 Human genes 0.000 description 3
- 230000002269 spontaneous effect Effects 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 230000001131 transforming effect Effects 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- 101150105382 MET gene Proteins 0.000 description 2
- 108700023175 Phosphate acetyltransferases Proteins 0.000 description 2
- 108010053763 Pyruvate Carboxylase Proteins 0.000 description 2
- 102100039895 Pyruvate carboxylase, mitochondrial Human genes 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 2
- KLOHDWPABZXLGI-YWUHCJSESA-M ampicillin sodium Chemical compound [Na+].C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C([O-])=O)(C)C)=CC=CC=C1 KLOHDWPABZXLGI-YWUHCJSESA-M 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 238000001976 enzyme digestion Methods 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- 230000034659 glycolysis Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000013067 intermediate product Substances 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 102220023256 rs387907547 Human genes 0.000 description 2
- 230000003248 secreting effect Effects 0.000 description 2
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 2
- 229960003487 xylose Drugs 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 244000153158 Ammi visnaga Species 0.000 description 1
- 235000010585 Ammi visnaga Nutrition 0.000 description 1
- 101100060007 Caenorhabditis elegans mig-22 gene Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 241000606790 Haemophilus Species 0.000 description 1
- 101000833492 Homo sapiens Jouberin Proteins 0.000 description 1
- 101000651236 Homo sapiens NCK-interacting protein with SH3 domain Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 102100024407 Jouberin Human genes 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 101100233669 Pseudomonas aeruginosa istB gene Proteins 0.000 description 1
- 108010042687 Pyruvate Oxidase Proteins 0.000 description 1
- 101001004672 Schizosaccharomyces pombe (strain 972 / ATCC 24843) Probable L-lactate dehydrogenase Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 102220369446 c.1274G>A Human genes 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000010531 catalytic reduction reaction Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000006114 decarboxylation reaction Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 101150040373 istA gene Proteins 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000006241 metabolic reaction Methods 0.000 description 1
- 230000001035 methylating effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011946 reduction process Methods 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Steroid Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (46)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0024554.8 | 2000-10-06 | ||
GBGB0024554.8A GB0024554D0 (en) | 2000-10-06 | 2000-10-06 | Ethanol production |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNA018195660A Division CN1798846A (zh) | 2000-10-06 | 2001-10-05 | 乙醇生产 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101775363A true CN101775363A (zh) | 2010-07-14 |
CN101775363B CN101775363B (zh) | 2016-08-03 |
Family
ID=9900827
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNA018195660A Pending CN1798846A (zh) | 2000-10-06 | 2001-10-05 | 乙醇生产 |
CN200910204036.0A Expired - Fee Related CN101775363B (zh) | 2000-10-06 | 2001-10-05 | 乙醇生产 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNA018195660A Pending CN1798846A (zh) | 2000-10-06 | 2001-10-05 | 乙醇生产 |
Country Status (6)
Country | Link |
---|---|
US (3) | US20020081677A1 (zh) |
CN (2) | CN1798846A (zh) |
AT (1) | ATE509114T1 (zh) |
DK (1) | DK1322775T3 (zh) |
GB (1) | GB0024554D0 (zh) |
ZA (1) | ZA200302998B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114410562A (zh) * | 2022-02-08 | 2022-04-29 | 安徽工程大学 | 一种克雷伯氏菌工程菌及其在生产乙醇中的应用 |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100376677C (zh) * | 2003-03-12 | 2008-03-26 | 株式会社味滋集团公司 | 醋酸菌的乙醇脱氢酶基因 |
NZ563043A (en) * | 2005-05-04 | 2010-04-30 | Tmo Renewables Ltd | Thermophilic microorganisms with inactivated lactate dehydrogenase gene (LDH) for ethanol production |
AP2013006819A0 (en) | 2006-07-21 | 2013-04-30 | Xyleco Inc | Conversion systems for biomass |
SG186645A1 (en) * | 2007-12-13 | 2013-01-30 | Glycos Biotechnologies Inc | Microbial conversion of oils and fatty acids to high-value chemicals |
US20090246841A1 (en) * | 2008-03-26 | 2009-10-01 | Jamieson Andrew C | Methods and compositions for production of acetaldehyde |
EP2519628A2 (en) | 2009-12-29 | 2012-11-07 | Butamax (TM) Advanced Biofuels LLC | Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols |
BR112012018694A2 (pt) * | 2010-01-26 | 2015-09-15 | Scale Biofuel Aps | "métodos para a produção e a colheita de etanol e aparelho para a produção e a colheita do mesmo." |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58147540A (ja) | 1982-02-26 | 1983-09-02 | Hitachi Ltd | 薄膜永久磁石の製造方法 |
US5482846A (en) * | 1988-08-31 | 1996-01-09 | University Of Florida | Ethanol production in Gram-positive microbes |
ES2095233T3 (es) | 1989-08-11 | 1997-02-16 | Gist Brocades Nv | Produccion eficaz de proteasa mutante. |
JP2931939B2 (ja) | 1992-07-10 | 1999-08-09 | 明治乳業株式会社 | Lactobacillus delbrueckii 種染色体への遺伝子組込み方法及び遺伝子組込み体 |
US5827515A (en) | 1995-08-30 | 1998-10-27 | Sandoz Ltd. | Bacillus thuringiensis sporulation gene |
GB0000185D0 (en) | 2000-01-06 | 2000-03-01 | Agrol Limited | Ethanol production |
-
2000
- 2000-10-06 GB GBGB0024554.8A patent/GB0024554D0/en not_active Ceased
-
2001
- 2001-10-05 DK DK01972318.8T patent/DK1322775T3/da active
- 2001-10-05 CN CNA018195660A patent/CN1798846A/zh active Pending
- 2001-10-05 AT AT01972318T patent/ATE509114T1/de not_active IP Right Cessation
- 2001-10-05 US US09/971,361 patent/US20020081677A1/en not_active Abandoned
- 2001-10-05 CN CN200910204036.0A patent/CN101775363B/zh not_active Expired - Fee Related
-
2003
- 2003-04-16 ZA ZA200302998A patent/ZA200302998B/en unknown
-
2005
- 2005-04-19 US US11/108,870 patent/US20050181492A1/en not_active Abandoned
-
2009
- 2009-02-24 US US12/391,366 patent/US8097460B2/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114410562A (zh) * | 2022-02-08 | 2022-04-29 | 安徽工程大学 | 一种克雷伯氏菌工程菌及其在生产乙醇中的应用 |
CN114410562B (zh) * | 2022-02-08 | 2023-05-30 | 安徽工程大学 | 一种克雷伯氏菌工程菌及其在生产乙醇中的应用 |
Also Published As
Publication number | Publication date |
---|---|
CN101775363B (zh) | 2016-08-03 |
US20050181492A1 (en) | 2005-08-18 |
ZA200302998B (en) | 2006-07-26 |
ATE509114T1 (de) | 2011-05-15 |
GB0024554D0 (en) | 2000-11-22 |
DK1322775T3 (da) | 2011-08-29 |
CN1798846A (zh) | 2006-07-05 |
US20020081677A1 (en) | 2002-06-27 |
US8097460B2 (en) | 2012-01-17 |
US20100190259A1 (en) | 2010-07-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5916787A (en) | Ethanol production in gram-positive microbes | |
CA1335430C (en) | Ethanol production by engineered microbes | |
CN103502435B (zh) | 重组微生物及其用途 | |
JP5732083B2 (ja) | エタノール産生 | |
US7691620B2 (en) | Ethanol production | |
CN101883861A (zh) | 利用hima表达减少的重组发酵单胞菌属菌株从包含木糖的培养基生产乙醇的方法 | |
US20120101304A1 (en) | Cells and method for producing acetone | |
CN101970671A (zh) | 在包含浓缩糖和乙酸盐的培养基中具有改进的乙醇产量的发酵单胞菌属 | |
US8097460B2 (en) | Ethanol production in bacillus | |
CN101448948A (zh) | 微生物乙醇生产的增强 | |
US6849434B2 (en) | Ethanol production in recombinant hosts | |
WO2007110592A2 (en) | Fermentation process for the production of ethanol | |
US20050158836A1 (en) | Ethanol production in gram-positive microbes | |
CN101868548A (zh) | 将木质纤维素生物质转化为乙醇的嗜热生物体 | |
AU2007231884B2 (en) | Ethanol production | |
Tanner | The microbial conversion of synthesis gas to ethanol: Characterization and optimization. | |
Liou | The microbial conversion of synthesis gas to ethanol: Characterization and optimization | |
Begum | Genetic and fermentation studies related to conversion of xylose to ethanol | |
Desai | Metabolic engineering of catabolic product metabolism in thermophilic bacteria |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CI01 | Publication of corrected invention patent application |
Correction item: Agent Correct: Yang Qing False: Yang Qing Number: 28 Volume: 26 |
|
CI02 | Correction of invention patent application |
Correction item: Agent Correct: Yang Qing False: Yang Qing Number: 28 Page: The title page Volume: 26 |
|
REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1143834 Country of ref document: HK |
|
CI01 | Publication of corrected invention patent application |
Correction item: Biological Conservation Information Correct: 2 Tiao False: 4 Tiao Number: 45 Volume: 26 |
|
CI02 | Correction of invention patent application |
Correction item: Biological Conservation Information Correct: 2 Tiao False: 4 Tiao Number: 45 Page: The title page Volume: 26 |
|
ERR | Gazette correction |
Free format text: CORRECT: BIOLOGICAL PRESERVATION INFORMATION; FROM: 4 ITEMS TO: 2 ITEMS |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
REG | Reference to a national code |
Ref country code: HK Ref legal event code: WD Ref document number: 1143834 Country of ref document: HK |
|
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160803 Termination date: 20161005 |