CN101748163B - 一种微生物发酵法生产丙酸及其盐的方法 - Google Patents
一种微生物发酵法生产丙酸及其盐的方法 Download PDFInfo
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- CN101748163B CN101748163B CN 200910248741 CN200910248741A CN101748163B CN 101748163 B CN101748163 B CN 101748163B CN 200910248741 CN200910248741 CN 200910248741 CN 200910248741 A CN200910248741 A CN 200910248741A CN 101748163 B CN101748163 B CN 101748163B
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- fermentation
- propionic acid
- culture
- salt
- propionate
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- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 title claims abstract description 94
- 238000000855 fermentation Methods 0.000 title claims abstract description 50
- 230000004151 fermentation Effects 0.000 title claims abstract description 49
- 235000019260 propionic acid Nutrition 0.000 title claims abstract description 46
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 31
- 244000005700 microbiome Species 0.000 title claims abstract description 11
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 title abstract 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 37
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 21
- 238000004519 manufacturing process Methods 0.000 claims abstract description 21
- 235000011187 glycerol Nutrition 0.000 claims abstract description 16
- 239000000243 solution Substances 0.000 claims abstract description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000007864 aqueous solution Substances 0.000 claims abstract description 9
- 238000005516 engineering process Methods 0.000 claims abstract description 9
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 8
- 241000186429 Propionibacterium Species 0.000 claims abstract description 8
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims abstract description 7
- 239000000920 calcium hydroxide Substances 0.000 claims abstract description 7
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims abstract description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims abstract description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 4
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 4
- 239000007921 spray Substances 0.000 claims abstract description 4
- 150000003839 salts Chemical class 0.000 claims description 29
- 239000003513 alkali Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 8
- 230000000813 microbial effect Effects 0.000 claims description 8
- 238000010899 nucleation Methods 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 238000011218 seed culture Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 4
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- 239000010452 phosphate Substances 0.000 claims description 4
- 239000011591 potassium Substances 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 239000000376 reactant Substances 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 244000068988 Glycine max Species 0.000 claims description 2
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- 102000004142 Trypsin Human genes 0.000 claims description 2
- 108090000631 Trypsin Proteins 0.000 claims description 2
- 238000002835 absorbance Methods 0.000 claims description 2
- 230000001133 acceleration Effects 0.000 claims description 2
- 238000012856 packing Methods 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 229910001220 stainless steel Inorganic materials 0.000 claims description 2
- 239000010935 stainless steel Substances 0.000 claims description 2
- 239000012588 trypsin Substances 0.000 claims description 2
- 239000012533 medium component Substances 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 2
- 235000013373 food additive Nutrition 0.000 abstract description 2
- 239000002778 food additive Substances 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- 239000012670 alkaline solution Substances 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- BCZXFFBUYPCTSJ-UHFFFAOYSA-L Calcium propionate Chemical compound [Ca+2].CCC([O-])=O.CCC([O-])=O BCZXFFBUYPCTSJ-UHFFFAOYSA-L 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 235000010331 calcium propionate Nutrition 0.000 description 3
- 239000004330 calcium propionate Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 235000012204 lemonade/lime carbonate Nutrition 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Natural products CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 2
- NBBJYMSMWIIQGU-UHFFFAOYSA-N Propionic aldehyde Chemical compound CCC=O NBBJYMSMWIIQGU-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000003260 anti-sepsis Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- 239000001273 butane Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000009713 electroplating Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 1
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- BWILYWWHXDGKQA-UHFFFAOYSA-M potassium propanoate Chemical compound [K+].CCC([O-])=O BWILYWWHXDGKQA-UHFFFAOYSA-M 0.000 description 1
- 235000010332 potassium propionate Nutrition 0.000 description 1
- 239000004331 potassium propionate Substances 0.000 description 1
- 239000001294 propane Substances 0.000 description 1
- 239000011814 protection agent Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- XDWXRAYGALQIFG-UHFFFAOYSA-L zinc;propanoate Chemical compound [Zn+2].CCC([O-])=O.CCC([O-])=O XDWXRAYGALQIFG-UHFFFAOYSA-L 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
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CN 200910248741 CN101748163B (zh) | 2009-12-25 | 2009-12-25 | 一种微生物发酵法生产丙酸及其盐的方法 |
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CN101748163A CN101748163A (zh) | 2010-06-23 |
CN101748163B true CN101748163B (zh) | 2012-12-12 |
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Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012076621A1 (en) * | 2010-12-07 | 2012-06-14 | Purac Biochem Bv | Fruit ferments containing propionate and use thereof |
CN102268463B (zh) * | 2011-06-09 | 2012-12-19 | 太原科技大学 | 一种从酒精酒糟废水中制取丙酸的方法 |
CN102766589B (zh) * | 2012-05-25 | 2013-10-16 | 江南大学 | 一株产丙酸詹氏丙酸杆菌及其应用 |
CN102703530A (zh) * | 2012-06-08 | 2012-10-03 | 沈阳科纳提克生物科技有限公司 | 一种丙酸盐的生产工艺 |
CN103667373B (zh) * | 2012-09-05 | 2016-05-11 | 深圳市绿微康生物工程有限公司 | 微生物发酵产丙酸及其盐联产丁二酸及其盐的方法 |
CN102919551A (zh) * | 2012-11-22 | 2013-02-13 | 生物源生物技术(深圳)有限公司 | 微生物发酵法生产丙酸型防霉剂的方法及其用途 |
EP3152314A1 (en) * | 2014-06-06 | 2017-04-12 | PURAC Biochem BV | Glycerol-based fermentation process |
US10723681B2 (en) | 2014-07-09 | 2020-07-28 | Purac Biochem B.V. | Process for manufacturing purified glycerol |
EP3070173A1 (en) * | 2015-03-18 | 2016-09-21 | PURAC Biochem BV | Process for manufacturing propionate products |
WO2017059887A1 (en) | 2015-10-05 | 2017-04-13 | Purac Biochem Bv | Glycerol-based fermentation process |
CN106868064A (zh) * | 2015-12-14 | 2017-06-20 | 沈阳华美畜禽有限公司 | 一种微生物发酵丙酸及其盐的培养基 |
CN106868063A (zh) * | 2015-12-14 | 2017-06-20 | 沈阳华美畜禽有限公司 | 一种微生物发酵丙酸及其盐的工艺 |
CN106868062A (zh) * | 2015-12-14 | 2017-06-20 | 沈阳华美畜禽有限公司 | 一种发酵丙酸及其盐的方法 |
CN106635875A (zh) * | 2016-10-17 | 2017-05-10 | 沈阳科纳提克生物科技有限公司 | 培养基及其在发酵生产丙酸中的应用 |
CN107227321A (zh) * | 2017-06-02 | 2017-10-03 | 合肥华盖生物科技有限公司 | 一种微生物发酵法生产丙酸盐防霉剂生产方法及用途 |
CN111549080A (zh) * | 2020-06-12 | 2020-08-18 | 陕西科技大学 | 一种丙酸锌的发酵生产工艺 |
CN112176005B (zh) * | 2020-10-27 | 2022-04-29 | 清远一生自然生物研究院有限公司 | 一种丙酸钙的生产方法 |
-
2009
- 2009-12-25 CN CN 200910248741 patent/CN101748163B/zh active Active
Non-Patent Citations (5)
Title |
---|
E. H. Himmi等.Propionic acid fermentation of glycerol and glucose by Propionibacterium acidipropionici and Propionibacterium freudenreichii ssp. shermanii.《Appl Microbiol Biotechnol》.2000,435-440. * |
Jefferson Coral等.Batch Fermentation Model of Propionic Acid Production by Propionibacterium acidipropionici in Different Carbon Sources.《Appl Biochem Biotechnol》.2008,333-341. * |
V. Goswami等.Propionic acid production in an in situ cell retention bioreactor.《Appl Microbiol Biotechnol》.2001,676-680. * |
张华峰等.微生物发酵法生产丙酸.《饲料工业》.2004,第25卷(第8期),29-33. * |
熊海燕.微生物发酵法生产丙酸的研究进展.《江苏调味副食品》.2005,第22卷(第5期),10-13. * |
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