CN101731199A - Preserving liquid for swine sperm - Google Patents
Preserving liquid for swine sperm Download PDFInfo
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- CN101731199A CN101731199A CN200910155880A CN200910155880A CN101731199A CN 101731199 A CN101731199 A CN 101731199A CN 200910155880 A CN200910155880 A CN 200910155880A CN 200910155880 A CN200910155880 A CN 200910155880A CN 101731199 A CN101731199 A CN 101731199A
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Abstract
The invention relates to preserving liquid for sperm, in particular to the preserving liquid for swine sperm, and belongs to the technical field of animal reproduction. The preserving liquid for the swine sperm comprises the following components: 25.5 to 35.0g/l of anhydrous dextrose, 5.1 to 7.0 g/l of trisodium citrate, 1 to 10 g/l of sodium bicarbonate, 2 to 4g/l of EDTA, 1 to 3g/l of potassium chloride, 2.4 to 3.4g/l of citric acid, 8.5 to 9.5g/l of trihydroxymethylaminomethane, 0.26 to 1g/l of amikacin and 0.2 to 1g/l of vitamin C. Through detection, in the swine sperm preserved by the preserving liquid, the sperm viability reaches 0.60 to 0.85 and the integrity rate of sperm acrosome reaches 90 percent, which excess the requirement of the current artificial fertilization.
Description
Technical field
The present invention relates to a kind of seminal fluid and preserve liquid, particularly the preservation liquid of a boar seminal fluid belongs to the animal reproduction technical field.
Technical background
Artificial insemination can be described as and improves the most important management work of livestock and poultry reproduction rate.From 20 beginnings of the century, the kind horse breeder Sand of Denmark and Stribolt with artificial insemination as commercial instrument with since maximally utilising good species horse seminal fluid, technology of artificial insemination on horse, poultry, ox and sheep produce by large-scale application.
The artificial fertilization technology has the following advantages: 1. good breeding stock (fowl) seminal fluid that is used on genetics inseminates for numerous dams (fowl), makes genetic improvement speed be significantly improved; 2. improve the health of livestock and poultry, the biosafety level on raising pig farm by the propagation of controlling some pathogene; 3. reduce the number of animals raised of male animal (fowl), reduce production costs.
The preservation technology of seminal fluid is one of key technology of being achieved of artificial insemination.Existing seminal fluid store method can be divided into normal temperature (15-25 ℃) preservation, low temperature (0-5 ℃) preservation and ultralow temperature (79～196 ℃) and preserve three kinds.Wherein, it is that seminal fluid is kept under the room temperature of certain amplitude of fluctuation that normal temperature is preserved (15-25 ℃), so also claim alternating temperature to preserve or room temperature preservation.Because the pig sperm is very responsive to low temperature, particularly sharply is cooled to 10-0 ℃ from body temperature, can make the irreversible cold shock phenomenon of spermatogenesis, therefore, low temperature is preserved and ultralow temperature is preserved less aborning application.And that normal temperature is preserved required equipment is simple, is convenient to penetration and promotion, is suitable for pig complete set seminal fluid especially and preserves.
But under the temperature condition (15-25 ℃) that normal temperature is preserved, the metabolism and the inhibition microbial growth that reduce sperm all compare difficulty, and diluting boar semen directly has influence on the sperm time-to-live with the quality of preserving liquid.It is shorter that existing porcine semen at normal temperature preservation liquid exists the holding time, causes seminal fluid to be wasted in a large number; Simultaneously, the pig seminal fluid of external import is preserved liquid and is cost an arm and a leg, and can not satisfy the demand in Chinese vast rural area and pastoral area.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, aim to provide a kind of pig seminal fluid, prolongation seminal fluid holding time and lower-cost pig seminal fluid can effectively preserved and preserve liquid.
For this reason, the present invention adopts following technical scheme:
The preservation liquid of one boar seminal fluid, it is characterized in that its set of dispense than (by weight) is: DEXTROSE ANHYDROUS 25.5～35.0g/l, two hydration trisodium citrates, 5.1～7.0g/l, sodium bicarbonate 1～10g/l, EDTA 2～4g/l, potassium chloride 1～3g/l, citric acid 2.4～3.4g/l, TRIS (trishydroxymethylaminomethane) 8.5～9.5g/l, AMK (amikacin) 0.26～1g/l, V-C (vitamin C) 0.2～1g/l.
As preferred ingredients proportioning (by weight) be: DEXTROSE ANHYDROUS 30.0～35.0g/l, two hydration trisodium citrates, 6.2～7.0g/l, sodium bicarbonate 4～10g/l, EDTA 3～4g/l, potassium chloride 2～3g/l, citric acid 3.0～3.4g/l, TRIS 8.7～9.2g/l, AMK 0.6～1g/l, V-C 0.6～1g/l.
As preferably, the present invention can also comprise that concentration is 5 * 10
-6～5 * 10
-5The epiphysin of mol/l (Melatonin, MLT).
As preferably, the concentration of described epiphysin is 1 * 10
-5Mol/l.
Preserve the pig seminal fluid that liquid is preserved with pig seminal fluid of the present invention, its sperm viability reaches 0.60-0.85 after testing, and the perforatorium percentage of head rice reaches 90%, surpasses the requirement of present artificial insemination.
Embodiment
The preservation liquid of 1: one boar seminal fluid of embodiment, its set of dispense than (by weight) is: DEXTROSE ANHYDROUS 25.5g/l, two hydration trisodium citrate 5.1g/l, sodium bicarbonate 2g/l, EDTA 2g/l, potassium chloride 1g/l, citric acid 2.4g/l, TRIS 8.5g/l, AMK 0.26g/l, V-C 0.2g/l.
The preparation method:
(1) metering takes by weighing DEXTROSE ANHYDROUS 25.5g, two hydration trisodium citrate 5.1g, sodium bicarbonate 2g, EDTA 2g, potassium chloride 1g, citric acid 2.4g, TRIS 8.5g, AMK 0.26g, V-C 0.2g respectively;
(2) component of step (1) is inserted in the container, adding distil water is dissolved to 1 liter, shakes up;
(3) solution that step (2) is obtained filters, and obtains preserving liquid, regulates pH value to 7.2-7.4;
When (4) using, add 2 parts of ratios of preserving liquid with 1 part of seminal fluid and use, and be kept under 16～22 ℃ the temperature environment.
The preservation liquid of 2: one boar seminal fluid of embodiment, its set of dispense than (by weight) is: DEXTROSE ANHYDROUS 35.0g/l, two hydration trisodium citrate 7.0g/l, sodium bicarbonate 10g/l, EDTA 4g/l, potassium chloride 3g/l, citric acid 3.4g/l, TRIS 9.5g/l, AMK 1g/l, V-C 1g/l.
The preparation method is with embodiment 1.
The preservation liquid of 3: one boar seminal fluid of embodiment, its set of dispense than (by weight) is: DEXTROSE ANHYDROUS 30.0g/l, two hydration trisodium citrate 6.0g/l, sodium bicarbonate 5.5g/l, EDTA 3g/l, potassium chloride 2g/l, citric acid 3.0g/l, TRIS 9.0g/l, AMK 0.6g/l, V-C 0.6g/l.
The preparation method is with embodiment 1.
The preservation liquid of 4: one boar seminal fluid of embodiment, its set of dispense than (by weight) is: DEXTROSE ANHYDROUS 32.5g/l, two hydration trisodium citrate 6.7g/l, sodium bicarbonate 6g/l, EDTA 3g/l, potassium chloride 2.5g/l, citric acid 3.0g/l, TRIS 9.0g/l, AMK 0.7g/l, V-C 0.8g/l.
The preparation method is with embodiment 1.
Embodiment 5: adding concentration on the basis of embodiment 4 is 1 * 10
-5The epiphysin of mol/l (Melatonin, MLT).
The preservation liquid of different embodiments of the invention, the effect of preservation pig seminal fluid is (preserving formula of liquid with the BTS seminal fluid is contrast) as shown in Table 1 and Table 2:
Table 1 pig seminal fluid is preserved sperm viability and acrosomal integrity after 7 days
| Preservation liquid numbering | Sperm viability | The perforatorium percentage of head rice |
| The preservation liquid of embodiment 1 | ??0.60 | ??0.89 |
| The preservation liquid of embodiment 2 | ??0.63 | ??0.88 |
| Preservation liquid numbering | Sperm viability | The perforatorium percentage of head rice |
| The preservation liquid of embodiment 3 | ??0.70 | ??0.89 |
| The preservation liquid of embodiment 4 | ??0.75 | ??0.90 |
| The preservation liquid of embodiment 5 | ??0.85 | ??0.92 |
| Contrast (BTS) | ??0.47 | ??0.81 |
Table 2 preservation liquid of the present invention and BTS preserve liquid artificial insemination and natural mating effect are compared
At last, it is also to be noted that what more than enumerate only is some specific embodiments of the present invention.If those of ordinary skills from all distortion that content disclosed by the invention directly derives or associates, all should think protection scope of the present invention.
Claims (5)
1. the preservation liquid of a boar seminal fluid, it is characterized in that its set of dispense than (by weight) is: DEXTROSE ANHYDROUS 25.5～35.0g/l, two hydration trisodium citrates, 5.1～7.0g/l, sodium bicarbonate 1～10g/l, EDTA2～4g/l, potassium chloride 1～3g/l, citric acid 2.4～3.4g/l, trishydroxymethylaminomethane 8.5～9.5g/l, amikacin 0.26～1g/l, vitamin C 0.2～1g/l.
2. the preservation liquid of pig seminal fluid according to claim 1, it is characterized in that its set of dispense than (by weight) is: DEXTROSE ANHYDROUS 30.0～35.0g/l, two hydration trisodium citrates, 6.2～7.0g/l, sodium bicarbonate 4～10g/l, EDTA 3～4g/l, potassium chloride 2～3g/l, citric acid 3.0～3.4g/l, trishydroxymethylaminomethane 8.7～9.2g/l, amikacin 0.6～1g/l, vitamin C 0.6～1g/l.
3. the preservation liquid of pig seminal fluid according to claim 1, it is characterized in that its set of dispense than (by weight) is: DEXTROSE ANHYDROUS 32.5g/l, two hydration trisodium citrate 6.7g/l, sodium bicarbonate 6g/l, EDTA3g/l, potassium chloride 2.5g/l, citric acid 3.0g/l, trishydroxymethylaminomethane 9.0g/l, amikacin 0.7g/l, vitamin C 0.8g/l.
4. according to the preservation liquid of the arbitrary described pig seminal fluid of claim 1-3, it is characterized in that: comprise that also concentration is 5 * 10
-6～5 * 10
-5The epiphysin of mol/l.
5. the preservation liquid of pig seminal fluid according to claim 4 is characterized in that: the concentration of described epiphysin is 1 * 10
-5Mol/l.
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| CN2009101558809A CN101731199B (en) | 2009-12-28 | 2009-12-28 | Preserving liquid for swine sperm |
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| CN2009101558809A CN101731199B (en) | 2009-12-28 | 2009-12-28 | Preserving liquid for swine sperm |
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| CN101731199B CN101731199B (en) | 2012-10-31 |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856016A (en) * | 2010-06-19 | 2010-10-13 | 张尤嘉 | Diluent for preserving porcine semen at normal temperature |
| CN102318596A (en) * | 2011-07-12 | 2012-01-18 | 青岛康大食品有限公司 | Diluent for preserving rabbit sperms at normal temperature and preparation method thereof |
| CN102613168A (en) * | 2012-03-05 | 2012-08-01 | 深圳市安多福动物药业有限公司 | Disinfectant for animal semen and preparation method thereof |
| CN102687715A (en) * | 2011-03-23 | 2012-09-26 | 北京浩邦猪人工授精服务有限责任公司 | Cold-water instant pig seminal fluid dilution powder formula and manufacturing process and application method |
| CN102919218A (en) * | 2012-11-21 | 2013-02-13 | 湖北维达健基因技术有限公司 | Composite for preservation of human saliva and preparation method there of |
| CN104721184A (en) * | 2015-02-16 | 2015-06-24 | 中国农业大学 | Preparation capable of improving cow conception rate as well as preparation method and application of preparation |
| WO2015193265A1 (en) | 2014-06-16 | 2015-12-23 | Università degli Studi di Parma | Composition for extenders for the long-term conservation of animal seminal material |
| CN105532645A (en) * | 2016-01-18 | 2016-05-04 | 赵彪 | Animal semen culture agent achieving constant-temperature preservation |
| CN107372460A (en) * | 2017-07-13 | 2017-11-24 | 漳州傲农现代农业开发有限公司 | A kind of porcine semen at normal temperature of anti-oxidation stress preserves diluent, preserves liquid and application |
| CN108588014A (en) * | 2018-03-15 | 2018-09-28 | 广州伯瑞丁生物新技术开发有限公司 | A kind of Boar spermatozoa culture medium and preparation method thereof |
| CN108812646A (en) * | 2018-08-01 | 2018-11-16 | 公安部南昌警犬基地 | A kind of stored refrigerated dilution of dog sperm |
| CN111436420A (en) * | 2020-04-14 | 2020-07-24 | 武汉市金驰海科技有限公司 | Long-acting fresh pig semen diluting preservative and using method thereof |
| JP2021500927A (en) * | 2017-10-30 | 2021-01-14 | プレミアム ジェネティクス （ユーケー） リミテッド | Compositions and Methods for Improving Gamete Viability and Function |
| CN113615682A (en) * | 2021-09-05 | 2021-11-09 | 内蒙古赛诺种羊科技有限公司 | Sheep hypertonic complexing agent semen dilution preserving fluid and application method thereof |
| CN114794086A (en) * | 2022-06-08 | 2022-07-29 | 杭州东源生物科技有限公司 | Anti-coagulation pig semen dilution powder and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3364680B2 (en) * | 1998-10-07 | 2003-01-08 | 山梨県 | Diluent for swine semen storage and dilution of swine semen |
| CN101543208B (en) * | 2009-03-31 | 2012-05-30 | 华南农业大学 | Powder for diluting and storing boar semen at normal temperature and preparation method and application thereof |
-
2009
- 2009-12-28 CN CN2009101558809A patent/CN101731199B/en active Active
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856016A (en) * | 2010-06-19 | 2010-10-13 | 张尤嘉 | Diluent for preserving porcine semen at normal temperature |
| CN101856016B (en) * | 2010-06-19 | 2013-03-13 | 张尤嘉 | Diluent for preserving porcine semen at normal temperature |
| CN102687715A (en) * | 2011-03-23 | 2012-09-26 | 北京浩邦猪人工授精服务有限责任公司 | Cold-water instant pig seminal fluid dilution powder formula and manufacturing process and application method |
| CN102318596A (en) * | 2011-07-12 | 2012-01-18 | 青岛康大食品有限公司 | Diluent for preserving rabbit sperms at normal temperature and preparation method thereof |
| CN102613168A (en) * | 2012-03-05 | 2012-08-01 | 深圳市安多福动物药业有限公司 | Disinfectant for animal semen and preparation method thereof |
| CN102919218A (en) * | 2012-11-21 | 2013-02-13 | 湖北维达健基因技术有限公司 | Composite for preservation of human saliva and preparation method there of |
| WO2015193265A1 (en) | 2014-06-16 | 2015-12-23 | Università degli Studi di Parma | Composition for extenders for the long-term conservation of animal seminal material |
| CN104721184A (en) * | 2015-02-16 | 2015-06-24 | 中国农业大学 | Preparation capable of improving cow conception rate as well as preparation method and application of preparation |
| CN105532645A (en) * | 2016-01-18 | 2016-05-04 | 赵彪 | Animal semen culture agent achieving constant-temperature preservation |
| CN107372460A (en) * | 2017-07-13 | 2017-11-24 | 漳州傲农现代农业开发有限公司 | A kind of porcine semen at normal temperature of anti-oxidation stress preserves diluent, preserves liquid and application |
| JP2021500927A (en) * | 2017-10-30 | 2021-01-14 | プレミアム ジェネティクス （ユーケー） リミテッド | Compositions and Methods for Improving Gamete Viability and Function |
| CN108588014A (en) * | 2018-03-15 | 2018-09-28 | 广州伯瑞丁生物新技术开发有限公司 | A kind of Boar spermatozoa culture medium and preparation method thereof |
| CN108812646A (en) * | 2018-08-01 | 2018-11-16 | 公安部南昌警犬基地 | A kind of stored refrigerated dilution of dog sperm |
| CN111436420A (en) * | 2020-04-14 | 2020-07-24 | 武汉市金驰海科技有限公司 | Long-acting fresh pig semen diluting preservative and using method thereof |
| CN113615682A (en) * | 2021-09-05 | 2021-11-09 | 内蒙古赛诺种羊科技有限公司 | Sheep hypertonic complexing agent semen dilution preserving fluid and application method thereof |
| CN114794086A (en) * | 2022-06-08 | 2022-07-29 | 杭州东源生物科技有限公司 | Anti-coagulation pig semen dilution powder and application thereof |
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| CN101731199B (en) | 2012-10-31 |
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