CN103518703B - A kind of diluting boar semen agent - Google Patents
A kind of diluting boar semen agent Download PDFInfo
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- CN103518703B CN103518703B CN201310478237.6A CN201310478237A CN103518703B CN 103518703 B CN103518703 B CN 103518703B CN 201310478237 A CN201310478237 A CN 201310478237A CN 103518703 B CN103518703 B CN 103518703B
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- boar semen
- present
- thinner
- diluting boar
- agent
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Abstract
The present invention relates to a kind of thinner, particularly relate to a kind of diluting boar semen agent, belong to artificial insemination field.Diluting boar semen agent disclosed by the invention contains glucose, trisodium citrate, potassium chloride, polyvinyl alcohol, Pehanorm, citric acid, Ciprofloxacin, glutamine dipeptide and antifreeze protein.Use thinner of the present invention, its sperm viability reaches 0.87% after testing, and Sperm acrasomal integvity reaches 91.2%, and rate of teratosperm is minimum is 7.67%.
Description
Technical field
The present invention relates to a kind of thinner, particularly relate to a kind of diluting boar semen agent, belong to artificial insemination field.
Background technology
The important step of pig artificial fertilization technology during pig semen storage in vitro, and the quality of thinner is the principal element affecting the pig semen holding time.The thinner major part of current domestic employing is all the product from external import, sub-fraction is also had to be introduce production and processing voluntarily of filling a prescription, import price comparison is expensive, add aquaculture cost, so develop good, the economical and practical thinner of preservation effect to technology of artificial insemination with to reduce artificial insemination cost significant.
Summary of the invention
The object of the present invention is to provide a kind of diluting boar semen agent.
Object of the present invention is achieved through the following technical solutions:
A kind of diluting boar semen agent, is characterized in that: thinner contains glucose, trisodium citrate, potassium chloride, polyvinyl alcohol, trishydroxymethylaminomethane, citric acid, Ciprofloxacin, glutamine dipeptide.
Preferably: glucose content is 30g/L ~ 40g/L.
Preferably: trisodium citrate content is 4g/L ~ 10g/L.
Preferably: KCE content is 1g/L ~ 3g/L.
Preferably: polyvinyl alcohol content is 1.2g/L ~ 3.5g/L.
Preferably: trishydroxymethylaminomethane content is 10g/L ~ 15g/L.
Preferably: citric acid content is 3g/L ~ 6g/L.
Preferably: Ciprofloxacin content is 3g/L ~ 5g/L.
Preferably: glutamine dipeptide content is 0.6g/L ~ 1.5g/L.
Preferably: dilution also comprises antifreeze protein, content is 0.7g/L ~ 1.3g/L.
Diluting boar semen agent preservation effect of the present invention is good, and price is suitable for, and can meet the requirement of pig artificial insemination.
Embodiment
Below in conjunction with specific embodiment, elaboration detailed is further done to the present invention, but embodiments of the present invention are not limited to the scope that embodiment represents.These embodiments only for illustration of the present invention, but not for limiting the scope of the invention.In addition, after reading content of the present invention, those skilled in the art can do various amendment to the present invention, and these equivalent variations fall within appended claims limited range of the present invention equally.
Embodiment 1
30g glucose, 4g trisodium citrate, 1g potassium chloride, 1.2g polyvinyl alcohol, 10g trishydroxymethylaminomethane, 3g citric acid, 3g Ciprofloxacin, 0.6g glutamine dipeptide, 0.7g antifreeze protein are added 500mL distilled water and stir, add water to 1000mL, regulate PH to 6.8 ~ 7.3.About dilute for standard containing 100,000,000 sperms with every milliliter of smart agent of dilution during use, preserve for 16 DEG C ~ 18 DEG C.
Embodiment 2
35g glucose, 6g trisodium citrate, 2g potassium chloride, 2.4g polyvinyl alcohol, 13g trishydroxymethylaminomethane, 4g citric acid, 3.5g Ciprofloxacin, 0.8g glutamine dipeptide, 1.2g antifreeze protein are added 500mL distilled water and stir, add water to 1000mL, regulate PH to 6.8 ~ 7.3.About dilute for standard containing 100,000,000 sperms with every milliliter of smart agent of dilution during use, preserve for 16 DEG C ~ 18 DEG C.
Embodiment 3
40g glucose, 10g trisodium citrate, 3g potassium chloride, 3.5g polyvinyl alcohol, 15g trishydroxymethylaminomethane, 6g citric acid, 5g Ciprofloxacin, 1.5g glutamine dipeptide, 1.3g antifreeze protein are added 500mL distilled water and stir, add water to 1000mL, regulate PH to 6.8 ~ 7.3.Dilute for standard containing 100,000,000 sperms with every milliliter of dilution extract solution during use, preserve for 16 DEG C ~ 18 DEG C.
According to the thinner of different embodiment, it is as shown in the table for the effect of preservation pig semen:
Table 1 preserves sperm viability, Sperm acrasomal integvity and rate of teratosperm after 3 days for pig semen
| Sperm viability | Sperm acrasomal integvity | Rate of teratosperm | |
| The conserving liquid of embodiment 1 | 0.81% | 90.1% | 8.71% |
| The conserving liquid of embodiment 2 | 0.87% | 89.7% | 8.13% |
| The conserving liquid of embodiment 3 | 0.85% | 91.2% | 7.67% |
Sperm viability, rate of teratosperm and Sperm acrasomal integvity are the domestic common counters carrying out sperm quality evaluation at present.Sperm viability >=60% of the qualified normal temperature seminal fluid product of regulation in national standard " boar normal temperature seminal fluid ", rate of teratosperm≤20%, the term of validity >=72h.When result of the test shows to preserve seminal fluid 3d, the normal temperature seminal fluid after above-mentioned thinner dilution complies with the national standard requirements, and its preservation effect meets seminal fluid need of production.
Claims (1)
1. a diluting boar semen agent, it is characterized in that: thinner contains glucose 30g/L ~ 40g/L, trisodium citrate 4g/L ~ 10g/L, potassium chloride 1g/L ~ 3g/L, polyvinyl alcohol 1.2g/L ~ 3.5g/L, trishydroxymethylaminomethane 10g/L ~ 15g/L, citric acid 3g/L ~ 6g/L, Ciprofloxacin 3g/L ~ 5g/L, glutamine dipeptide 0.6g/L ~ 1.5g/L and antifreeze protein, described antifreeze protein content is 0.7g/L ~ 1.3g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310478237.6A CN103518703B (en) | 2013-10-14 | 2013-10-14 | A kind of diluting boar semen agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310478237.6A CN103518703B (en) | 2013-10-14 | 2013-10-14 | A kind of diluting boar semen agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103518703A CN103518703A (en) | 2014-01-22 |
| CN103518703B true CN103518703B (en) | 2015-08-19 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310478237.6A Expired - Fee Related CN103518703B (en) | 2013-10-14 | 2013-10-14 | A kind of diluting boar semen agent |
Country Status (1)
| Country | Link |
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| CN (1) | CN103518703B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104322483A (en) * | 2014-10-09 | 2015-02-04 | 广东中农联生物制药有限公司 | Boar antiviral long-acting semen diluent formula and preparation method |
| CN107333751A (en) * | 2017-07-25 | 2017-11-10 | 昆明医科大学第二附属医院 | A kind of cells frozen storing liquid containing antifreeze protein |
| CN107751185A (en) * | 2017-10-19 | 2018-03-06 | 界首市起点家庭农场 | One boar high-quality semen diluent |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7208265B1 (en) * | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
| CN100399890C (en) * | 2006-11-07 | 2008-07-09 | 山东农业大学 | Goat sperm liquid-state preservation method |
| CN102687715A (en) * | 2011-03-23 | 2012-09-26 | 北京浩邦猪人工授精服务有限责任公司 | Cold-water instant pig seminal fluid dilution powder formula and manufacturing process and application method |
| CN102599147A (en) * | 2012-03-03 | 2012-07-25 | 平顶山瑞祥牧业有限公司 | Diluting powder for artificial fertilization of pigs |
| CN102726367A (en) * | 2012-06-12 | 2012-10-17 | 宁波市畜牧工程技术研究服务中心 | Low-temperature protective agent of boar semen |
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2013
- 2013-10-14 CN CN201310478237.6A patent/CN103518703B/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN103518703A (en) | 2014-01-22 |
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Granted publication date: 20150819 Termination date: 20181014 |