CN101803595A - Diluent for storing the sperm of pig under normal temperature and preparation and dilution method thereof - Google Patents
Diluent for storing the sperm of pig under normal temperature and preparation and dilution method thereof Download PDFInfo
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- CN101803595A CN101803595A CN 201010133910 CN201010133910A CN101803595A CN 101803595 A CN101803595 A CN 101803595A CN 201010133910 CN201010133910 CN 201010133910 CN 201010133910 A CN201010133910 A CN 201010133910A CN 101803595 A CN101803595 A CN 101803595A
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Abstract
The invention relates to a diluent for storing the sperm of a pig under normal temperature, which is characterized by comprises the following components by weight ratio: 9-11 of glucose, 0.2-0.3 of ethylene diamine tetraacetic acid, 0.5-0.6 of trisodium citrate, 0.08-0.12 of 1570U/mg penicillin and 0.2-0.25 of 720U/mg streptomycin. The diluent is completely dissolved in distilled water with distilled water according to the dilution ratio that diluent of 1g is diluted with distilled water of 20ml, and dilution liquid with the pH of being 6.5-6.8 and the osmotic pressure of being 290-310mOsmo/L is obtained; the diluent of the sperm of the pig, produced by the formulation, can effectively prolong the holding time, has convenient use and low cost, is suitable for being used by an artificial insemination station of the pig and a large-scale pig farm, has good application effect in the long-term production, has wider application range than the imported sperm, and can lead the conception rate of a sow in the oestrous period when being applied to a foreign pig to enable conception rate of a sow to be up to 86.8 percent with the litter size of 11.2 heads.
Description
Technical field
The present invention relates to a kind of porcine semen at normal temperature and preserve thinner, be used for the artificial insemination of pig, belong to the animal reproduction technical field.
Background technology
When natural mating, boar 1 sow that can only breed for 1 time, and the boar semen of once gathering carries out artificial insemination through dilution process, 20-30 the sow that generally can breed, and the pregnancy rate of sow can not reduce.Scientific research proves that each giving when oestrusing the sow semen deposition contained hundred million motile sperms of 20-30 in the seminal fluid of input, can guarantee the pregnancy rate of sow.According to up-to-date scientific research, the quality of boar semen inspection, the sperm of straight ahead motion accounts for total number of sperm between 60%-95%, accepts the pregnancy rate of artificial insemination sow and does not have significant difference.Common method by the International Evaluation motility of sperm can be divided into 4 grades.A level sperm is quick forward travel, and B level sperm is forward travel at a slow speed, and C level sperm is omnidirectional motion, and D level sperm is not for moving.Appliance computer sperm motility image analysis technology is measured, and in very high the reaching in 90% the fresh semen of the total vitality of subject of sperm, A level sperm generally is no more than 50%, and B level sperm is no more than 40%.In implementing secondary artificial insemination, as long as A level sperm accounts for 25% of total number of sperm, B level sperm accounts for 35%, nearly 4-5 hundred million A levels sperm and 5～600,000,000 B level sperms, and the pregnancy rate of sow can reach desirable index.When a volume of semen (1) is 300 milliliters, 2.8 hundred million/milliliter of sperm concentrations (2), sperm dilution preserve 2-4 days vigor (3) be 65%, first service reaches the motile sperm (4) more than 2,500,000,000.With (1) (2) (3) Xiang Liancheng, can get extension rate so divided by (4) item, can be with 22 parts of this sperm dilution.Along with scale and the fast development on pig farm on a fairly large scale, the modern technology of artificial insemination of pig begins large tracts of land to be promoted, but owing to lack high-quality sperm dilution preservative agent on the domestic market, and cost an arm and a leg from the semen diluent of external import, be difficult to popularize and use, and all have yolk in the thinner commonly used at present, and the portable pathogenic bacteria of yolk content cause easily pregnancy rate or farrowing rate low.Limited the penetration and promotion of technology of artificial insemination.
Given this, relevant expert in the institute is organized in the Sichuan Province Animal Husbandry Institute of Science and Technology, pull together to cooperate, on the basis of using for reference domestic and international pig breeding achievement in research, by the multiple formulations combined sorting, and a large amount of application tests, develop the diluting boar semen agent product of super quality and competitive price, and be widely used in production practices, and promoted the swine artificial insemination technical merit, brought good economic benefit for numerous raisers.
Summary of the invention
The objective of the invention is in order to overcome above-mentioned deficiency, provide a kind of porcine semen at normal temperature to preserve thinner, solve domestic no high-quality diluting boar semen agent, and the external too high present situation of import diluting boar semen agent price, to promote applying of swine artificial insemination technology.
In order to achieve the above object, the present invention has adopted following technical scheme:
A kind of porcine semen at normal temperature is preserved thinner and is made up of following components in weight percentage: the penicillin 0.08～0.12 of glucose 9～11, disodium ethylene diamine tetraacetate 0.2～0.3, trisodium citrate 0.5～0.6,1570U/mg, the streptomycin 0.2～0.25 of 720U/mg, evenly mix according to each component of the accurate weighing of aforementioned formula ratio, vacuum packaging promptly makes the present invention, the present invention is dissolved in distilled water according to the 1g thinner fully with the dilution ratio of 20ml distilled water diluting, obtaining pH is 6.5～6.8, and osmotic pressure is the dilution of 290～310mOsmo/L;
The porcine semen at normal temperature that the present invention preferably prepares is preserved thinner and is specifically comprised following composition in weight proportion: the penicillin 0.1 of glucose 10, disodium ethylene diamine tetraacetate 0.25, trisodium citrate 0.55,1570U/mg, the streptomycin 0.225 of 720U/mg, each component of the accurate weighing of aforementioned formula ratio is evenly mixed, then the present invention is dissolved in distilled water according to the 1g thinner fully with the dilution ratio of 20ml distilled water diluting, obtaining pH is 6.65, and osmotic pressure is the dilution of 300mOsmo/L.
It is standby more than half an hour that dilution after preparing places 35～37 ℃ water-bath to heat up, result of use the best after a hour.
The fresh semen of gathering should place 35～37 ℃ water-bath constant temperature immediately, is preferably in half an hour and carries out.Semen quality on inspection, vigor reaches can dilute more than 0.8 grade.Extension rate generally is advisable with 2～4 times, with 2 times of dilutions is example: as the fresh semen volume is 50 milliliters, then should measure 100 milliliters of above-mentioned dilutions (is former smart 1 part, 2 parts of dilutions) in beaker, slowly pour in dilution along walls of beaker fresh semen again, should accomplish during dilution that equality of temperature is with pressing, the temperature that is fresh semen has identical temperature and identical osmotic pressure with dilution, temperature generally is advisable with 35～37 ℃, dilution with the thinner preparation can guarantee the osmotic pressure identical with fresh semen.
Semen at normal temperature after the dilution is preserved (15～25 ℃), and optimum temperature is 17 ℃ (preferably using special-purpose seminal fluid insulating box).Seminal fluid after the dilution must slowly heat up in water-bath, treats that temperature reaches 35～37 ℃, and the microscopy vigor can semen deposition more than 0.5 grade.
Beneficial effect of the present invention is: the diluting boar semen agent of producing by this prescription; can improve effectively the sperm retention time; easy to use; and cheap, be suitable for swine artificial insemination station and large-scale pig farm and use, effect is good in long-term production; more wider than the utilization of import semen diluent; apply to outer boar and can make the sow non-return rate can reach 86.8%, the nest litter size can reach 11.2, obtains users' height praise.
Embodiment
Embodiment 1
With 4.5g glucose, 0.10g disodium ethylene diamine tetraacetate, 0.25g trisodium citrate, 0.04g the penicillin of 1570U/mg, the streptomycin of the 720U/mg of 0.1g, (beaker should boil sterilization in 30 minutes in advance to insert beaker after mixing, and use the distilled water rinse) in, add distilled water 90ml dissolving, gained dilution pH is 6.5, and osmotic pressure is 290mOsmo/L.
Embodiment 2
With 5g glucose, 0.125g disodium ethylene diamine tetraacetate, 0.275g trisodium citrate, 0.05g the penicillin of 1570U/mg, the streptomycin of the 720U/mg of 0.1125g, (beaker should boil sterilization in 30 minutes in advance to insert beaker, and use the distilled water rinse) in, add distilled water 100ml dissolving, gained dilution pH is 6.65, and osmotic pressure is 300mOsmo/L.
Embodiment 3
With 5.5g glucose, 0.15g disodium ethylene diamine tetraacetate, 0.3g trisodium citrate, 0.06g the penicillin of 1570U/mg, the streptomycin of the 720U/mg of 0.125g, (beaker should boil sterilization in 30 minutes in advance to insert beaker, and use the distilled water rinse) in, add distilled water 110ml dissolving, gained dilution pH is 6.8, and osmotic pressure is 310mOsmo/L.
The present invention dilutes pig seminal fluid experiment effect:
Table 1 is to adopt the present invention to dilute the pig seminal fluid to preserve its pig sperm viability after 3 days
Claims (5)
1. a porcine semen at normal temperature is preserved thinner, it is characterized in that this thinner is made up of following components in weight percentage: the penicillin 0.08～0.12 of glucose 9～11, disodium ethylene diamine tetraacetate 0.2～0.3, trisodium citrate 0.5～0.6,1570U/mg, the streptomycin 0.2～0.25 of 720U/mg.
2. porcine semen at normal temperature as claimed in claim 1 is preserved thinner, it is characterized in that the weight proportion of each component is: the penicillin 0.1 of glucose 10, disodium ethylene diamine tetraacetate 0.25, trisodium citrate 0.55,1570U/mg, the streptomycin 0.225 of 720U/mg.
3. prepare the method for claim 1 or 2 described thinners, it is characterized in that: evenly mix according to each component of the accurate weighing of aforementioned formula ratio, vacuum packaging promptly.
4. the dilution process of a thinner as claimed in claim 1, it is characterized in that: thinner is dissolved in distilled water according to the 1g thinner fully with the dilution ratio of 20ml distilled water diluting, obtaining pH is 6.5～6.8, and osmotic pressure is the dilution of 290～310mOsmo/L.
5. the dilution process of a thinner as claimed in claim 2 is characterized in that: thinner is dissolved in distilled water according to the 1g thinner fully with the dilution ratio of 20ml distilled water diluting, and obtaining pH is 6.65, and osmotic pressure is the dilution of 300mOsmo/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010133910 CN101803595A (en) | 2010-03-26 | 2010-03-26 | Diluent for storing the sperm of pig under normal temperature and preparation and dilution method thereof |
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| CN 201010133910 CN101803595A (en) | 2010-03-26 | 2010-03-26 | Diluent for storing the sperm of pig under normal temperature and preparation and dilution method thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613168A (en) * | 2012-03-05 | 2012-08-01 | 深圳市安多福动物药业有限公司 | Disinfectant for animal semen and preparation method thereof |
| CN102687715A (en) * | 2011-03-23 | 2012-09-26 | 北京浩邦猪人工授精服务有限责任公司 | Cold-water instant pig seminal fluid dilution powder formula and manufacturing process and application method |
| CN102726367A (en) * | 2012-06-12 | 2012-10-17 | 宁波市畜牧工程技术研究服务中心 | Low-temperature protective agent of boar semen |
| CN104770362A (en) * | 2015-03-12 | 2015-07-15 | 西北农林科技大学 | Application of schisandrin B in preparation of diluted liquid for preserving boar semen |
| WO2015193265A1 (en) | 2014-06-16 | 2015-12-23 | Università degli Studi di Parma | Composition for extenders for the long-term conservation of animal seminal material |
| CN105325401A (en) * | 2015-11-10 | 2016-02-17 | 天津市畜牧兽医研究所 | Fresh-pig-semen preserving long-acting dilution powder, preparing method thereof and application thereof |
| CN109258623A (en) * | 2018-09-18 | 2019-01-25 | 华中农业大学 | A kind of diluting boar semen agent and application |
| CN110393183A (en) * | 2018-04-24 | 2019-11-01 | 天津农学院 | Diluent and preparation method thereof and the application in porcine semen at normal temperature preservation |
| CN113273566A (en) * | 2021-05-31 | 2021-08-20 | 西北农林科技大学 | Application of glycine as effective component in boar semen normal-temperature preservation solution |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101392236A (en) * | 2008-11-06 | 2009-03-25 | 上海交通大学 | Antifreezer for diluting boar semen |
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2010
- 2010-03-26 CN CN 201010133910 patent/CN101803595A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101392236A (en) * | 2008-11-06 | 2009-03-25 | 上海交通大学 | Antifreezer for diluting boar semen |
Non-Patent Citations (2)
| Title |
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| 《上海畜牧兽医通讯》 20001231 尹雄鹰 不同稀释液及保存温度对猪精子存活时间的影响 28-29 1-5 , 第01期 * |
| 《甘肃畜牧兽医》 19931231 刘丑生等 猪精液常温条件下延长保存时间的研究 5-7 1-5 第23卷, 第2期 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687715A (en) * | 2011-03-23 | 2012-09-26 | 北京浩邦猪人工授精服务有限责任公司 | Cold-water instant pig seminal fluid dilution powder formula and manufacturing process and application method |
| CN102613168A (en) * | 2012-03-05 | 2012-08-01 | 深圳市安多福动物药业有限公司 | Disinfectant for animal semen and preparation method thereof |
| CN102726367A (en) * | 2012-06-12 | 2012-10-17 | 宁波市畜牧工程技术研究服务中心 | Low-temperature protective agent of boar semen |
| WO2015193265A1 (en) | 2014-06-16 | 2015-12-23 | Università degli Studi di Parma | Composition for extenders for the long-term conservation of animal seminal material |
| CN104770362A (en) * | 2015-03-12 | 2015-07-15 | 西北农林科技大学 | Application of schisandrin B in preparation of diluted liquid for preserving boar semen |
| CN105325401A (en) * | 2015-11-10 | 2016-02-17 | 天津市畜牧兽医研究所 | Fresh-pig-semen preserving long-acting dilution powder, preparing method thereof and application thereof |
| CN110393183A (en) * | 2018-04-24 | 2019-11-01 | 天津农学院 | Diluent and preparation method thereof and the application in porcine semen at normal temperature preservation |
| CN109258623A (en) * | 2018-09-18 | 2019-01-25 | 华中农业大学 | A kind of diluting boar semen agent and application |
| CN113273566A (en) * | 2021-05-31 | 2021-08-20 | 西北农林科技大学 | Application of glycine as effective component in boar semen normal-temperature preservation solution |
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