CN1289661C - Liquid for preserving pig semen - Google Patents
Liquid for preserving pig semen Download PDFInfo
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- CN1289661C CN1289661C CN 200410013364 CN200410013364A CN1289661C CN 1289661 C CN1289661 C CN 1289661C CN 200410013364 CN200410013364 CN 200410013364 CN 200410013364 A CN200410013364 A CN 200410013364A CN 1289661 C CN1289661 C CN 1289661C
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Abstract
The present invention specifically relates to a design and an application of preserving liquid of pig semen, which belongs to the technical field of pig propagation, and is characterized in that the preserving liquid contains dextrose, bovine serum albumin or no bovine serum albumin, potassium chloride or no potassium chloride, sodium citrate, edta, sodium bicarbonate, antibiotics and water. 80 to 100 ml of the preserving liquid is fed to one pig, and 3 billion live sperms are diluted, packaged separately, and preserved at a temperature of 17 DEG C; the activity is 0.53, the pH value is 7.4 or so, and the acrosome integrity rate of the sperms is 85% after 7 days; the conception rate of purebred sows inseminated within 7 days can reach more than 80% during the rut, the conception rate of cross-breeding sows can reach more than 85% during the rut, the number of live piglets rises by 0.5 to 1 per nest more than that of the live piglets by natural breeding, and the propagation rate of the sows rises by 5 to 10% per year, which reaches or exceeds the level of the natural breeding on present pig farms in China. The present invention can remarkably enhance the semen utilization of excellent service boars, can reduce the chances of disease spread, can reduce production cost, and simultaneously can avoid vicious circles, such as small radius of supplied semen caused by short preservation time of the semen, the shortening of the service life of boars, the decrease of frequent semen collection of the boars and the semen quality, etc., and other problems.
Description
Technical field
The invention belongs to the breeding technology field of pig, relate in particular to the seminal fluid preservation of pig and the preparation of diluent.
Background technology
From eighties of last century since the sixties, China has introduced a large amount of good pig kinds from external, for positive promoter action has been played in the development of Chinese pig industry.Along with the development of Swine Production, people require that good boar has that reproductive performance is good, fast growth, lean ratio height, adaptability is strong and feature such as good condition of health.Because swine disease is more and more obvious to the influence of boar, make some outstanding herd boars therefore be not fully utilized, cause the raising speed of breeding fraction of coverage comparatively slow.Adopt technology of artificial insemination can improve the utilization ratio of outstanding herd boar greatly, reduce the pathophoresis chance, reduce production costs.At present, the frozen semen technology of pig mainly is the bright smart semen deposition of using under the normal temperature preservation in the production still in conceptual phase.A test-tube major issue of using the bright capable pig of progressing greatly is exactly how to prolong survival time of boar sperm, to enlarge for smart scope as far as possible.In view of the pig sperm extremely responsive to cold shock, so frozen semen is very impossible for the breeding of pig.There is data to show that the pig seminal fluid of preserving more than 15 ℃ can reach the requirement to the sow insemination.Yet in this temperature range, reduce the metabolism of sperm and suppress microbial growth all relatively be stranded accurate (Zhang Zhongming. the application of long-acting diluent in swine artificial insemination. external Animal husbandry: pig and fowl .1990 (6) .-30-33; Huo Xiuxiang. screening of diluting boar semen liquid formula and store method test. Liaoning animal and veterinary .1994 (2) .-15-15).On the basis of skilled seminal fluid treatment technology accurately, the quality of diluent directly has influence on the sperm survival time.At present the liquid diluent kind of preserving of boar semen is a lot, its effect report differ (Ma Xiaolong etc. four kinds of diluents are tested pig seminal fluid preservation effect. the .1991 that raises pigs (2) .-11-12; Chen Dafang etc., the comparison test of diluent for preserving porcine semen at normal temperature prescription. Guizhou animal and veterinary .1998,22 (4) .-8-9; Dong Zaiyong, Qingyuan Pei. the diluent for preserving porcine semen at normal temperature formulation screening test. the journal .1995 of Guizhou Agriculture College, 14 (2) .-12-15 etc.); Exist cause because of the seminal fluid shelf time is short that seminal fluid is wasted in a large number, practical problemss such as the vicious cycle between the frequent semen collection of herd boar and semen quality decline and boar shortening working life.Simultaneously, the external semen dilution powder of import costs an arm and a leg, and can not apply in vast rural area of China and pastoral area.The applicant has carried out the shaker test that a series of pig seminal fluid are preserved and diluent is filled a prescription according to the Physiology and biochemistry characteristics of pig seminal fluid for this reason, is intended to filter out a kind of comparatively ideal pig seminal fluid and preserves and diluent.
Summary of the invention
The objective of the invention is to overcome the defective of prior art, prepare and a kind ofly can either preserve the seminal fluid of pig effectively, prevent the sperm inactivation, the pig seminal fluid that can obviously keep the insemination effect of this seminal fluid again and reduce the reproductive-cost of pig is preserved and diluent.
The present invention is achieved through the following technical solutions:
The preservation liquid of one boar seminal fluid, set of dispense ratio by weight is:
Glucose 37-60g/l; Bovine serum albumin 0.5-1.5g/l; Trisodium Citrate 3.7-6g/l; Ethylenediamine tetraacetic acid (EDTA) (EDTA) 1.3-3.7g/l; Sodium bicarbonate 1.2-1.3g/l; Repone K 0-0.4g/l; Penicillin 50-100 million international units; Streptomycin sulphate 50-100 million international units, adding distil water to 1 liter, pH7.2-7.4.
One of optimization of C is that set of dispense ratio by weight is:
Glucose 37g/l; Bovine serum albumin 0.5g/l; Trisodium Citrate 6g/l; EDTA 1.3g/l; Sodium bicarbonate 1.2g/l; Penicillin 100 million international units; Streptomycin sulphate 100 million international units, adding distil water to 1 liter, pH7.2-7.4.
Two of optimization of C is that set of dispense ratio by weight is:
Glucose 37g/l; Bovine serum albumin 1g/l; Trisodium Citrate 3.7g/l; EDTA 3.7g/l; Sodium bicarbonate 1.2g/l; Penicillin 100 million international units; Streptomycin sulphate 100 million international units, adding distil water to 1 liter, pH7.2-7.4.
Three of optimization of C is that set of dispense ratio by weight is:
Glucose 37g/l; Bovine serum albumin 1.5g/l; Trisodium Citrate 3.7g/l; EDTA3.7g/l; Sodium bicarbonate 1.2g/l; Repone K 0.4g/l; Penicillin 100 million international units; Streptomycin sulphate 100 million international units, adding distil water to 1 liter, pH7.2-7.4.
Eco-power prescription is: glucose 60g/l; Trisodium Citrate 6g/l; EDTA 3.7g/l; Sodium bicarbonate 1.2g/l; Repone K 0.4 gram; Penicillin 50 million international units; Streptomycin sulphate 50 million international units, adding distil water to 1 liter, pH7.2-7.4.
Positively effect of the present invention is
The diluting boar semen liquid that the present invention produces reaches 0.5-0.7 through the vigor that detects its sperm, and the perforatorium percentage of head rice surpasses 90%, surpasses present test-tube requirement.Simultaneously preservation liquid of the present invention has concurrently and preserves and diluting effect, the dilution seminal fluid of being prepared use and preserve all more convenient, can plug and play during artificial insemination, convenient and swift, can apply in vast rural area of China and professional pig farm.Implementation result shows that it is more remarkable than the effect of other similar diluent that the present invention preserves the seminal fluid of liquid preservation.Different ingredients of the present invention preparation pig seminal fluid preserve the liquid preparation seminal fluid effect as shown in Table 1 and Table 2:
Seminal fluid is preserved vigor, pH and acrosomal integrity after 7 days between table 1 diluent prescription
| Formula number | Motility of sperm | The pH of seminal fluid | Perforatorium percentage of head rice (%) |
| 001 | 0.53 | 7.42 | 0.85 |
| 002 | 0.59 | 7.44 | 0.89 |
| 003 | 0.64 | 7.40 | 0.87 |
| 004 | 0.63 | 7.44 | 0.86 |
| 005 | 0.75 | 7.43 | 0.90 |
| Contrast (BTS) | 0.47 | 7.41 | 0.81 |
Annotate: BTS is that the seminal fluid that u s company is recommended is preserved liquid formula, is that at present general seminal fluid is preserved liquid formula.
The applicant gives the sow semen deposition with preservation liquid that is numbered 002 formulated and the preservation liquid that is numbered 004 formulated respectively, and the key technical indexes such as their conception rate, litter size and natural crossing and the effect comparison of U.S. BTS diluent aspect artificial insemination are as shown in table 2.As can be seen from Table 2, use preservation liquid of the present invention and carry out liquid and natural crossing are preserved in artificial insemination respectively than BTS non-return rate high 1.7% and 2.3%, nest produces many 0.8 and 1.1 respectively of young number alive.
Table 2 seminal fluid of the present invention preserves liquid and BTS seminal fluid preservation liquid compares artificial insemination and natural crossing effect
| The breeding mode | Breeding sow number (head) | Non-return rate (%) | Nest produces the young number (head) of living |
| Use preservation liquid of the present invention and carry out artificial insemination | 3049 | 88.10 | 11.2 |
| Use the BTS diluent to carry out artificial insemination | 376 | 86.40 | 10.4 |
| Natural crossing | 939 | 85.80 | 10.1 |
Embodiment
Embodiment 1 pig normal temperature seminal fluid is preserved the preparation of liquid
(1) metering takes by weighing glucose 37g/l respectively; Bovine serum albumin 0.5g/l; Trisodium Citrate 6g/l; EDTA 1.3g/l; Sodium bicarbonate 1.2g/l; Penicillin 100 million international units; Streptomycin sulphate 100 million international units;
(2) component of step (1) is inserted in the container, added dissolved in distilled water to 1 liter, shake up;
(3) solution that step (2) is obtained filters, and obtains preserving liquid, transfers pH7.2-7.4;
(4) the preservation liquid that step (3) is obtained adds 2 parts of ratios of preserving liquid in 1 part of seminal fluid, slowly joins in the seminal fluid pig seminal fluid that obtains diluting;
(5) with above-mentioned dilution seminal fluid under 16-20 ℃ of condition, in preservation 4-7 days
Using method is: get 80 milliliters of the interior dilution seminal fluid of above-mentioned validity period, import the intrauterine of the sow that oestruses, promptly finish conventional artificial insemination.
Embodiment 2 pig normal temperature seminal fluid are preserved the preparation of liquid
According to embodiment 1 described step, the ratio of each component is of the preservation liquid of present embodiment:
Glucose 37g/l; Bovine serum albumin 1.5g/l; Trisodium Citrate 3.7g/l; EDTA 3.7g/l; Sodium bicarbonate 1.2g/1; Penicillin 50 million international units; Streptomycin sulphate 50 million international units, adding distil water to 1 liter, pH7.2-7.4.
Embodiment 3 pig normal temperature seminal fluid are preserved the preparation of liquid
According to embodiment 1 described step, the ratio of each component is of the preservation liquid of present embodiment:
Glucose 60g/l; Bovine serum albumin 1g/l; Trisodium Citrate 3.7g/l; EDTA 1.3g/l; Sodium bicarbonate 1.2g/l; Repone K 0.4 gram; Penicillin 100 million international units; Streptomycin sulphate 100 million international units, adding distil water to 1 liter, pH7.2-7.4.
Embodiment 4 pig normal temperature seminal fluid are preserved the preparation of liquid
According to embodiment 1 described step, the ratio of each component is of the preservation liquid of present embodiment:
Glucose 60g/l; Bovine serum albumin 0.8g/l; Trisodium Citrate 3.7g/l; EDTA 3.7g/l; Repone K 0.4g/l; Sodium bicarbonate 1.2g/l; Penicillin 100 million international units; Streptomycin sulphate 100 million international units, adding distil water to 1 liter, pH7.2-7.4
Embodiment 5 pig normal temperature seminal fluid are preserved the preparation of liquid
According to embodiment 1 described step, the ratio of each component is of the preservation liquid of present embodiment:
Glucose 60g/l; Trisodium Citrate 6g/l; EDTA 3.7g/l; Sodium bicarbonate 1.2g/l; Repone K 0.4g/l; Penicillin 50 million international units; Streptomycin sulphate 50 million international units, adding distil water to 1 liter, pH7.2-7.4.
Claims (5)
1, the preservation liquid of a boar seminal fluid is characterized in that, set of dispense ratio by weight is:
Glucose 37～60g/l; Bovine serum albumin 0.5-1.5g/l; Trisodium Citrate 3.7～6g/l; Ethylenediamine tetraacetic acid (EDTA) 1.3～3.7g/l; Sodium bicarbonate 1.2～1.3g/l; Repone K 0～0.4g/l; Penicillin 50-100 million international units; Streptomycin sulphate 50-100 million international units, adding distil water to 1 liter, pH7.2-7.4.
2, pig seminal fluid according to claim 1 is preserved liquid, it is characterized in that set of dispense ratio by weight is:
Glucose 37g/l; Bovine serum albumin 0.5g/l; Trisodium Citrate 6g/l; Ethylenediamine tetraacetic acid (EDTA) 1.3g/l; Sodium bicarbonate 1.2g/l; Penicillin 100 million international units; Streptomycin sulphate 100 million international units, adding distil water to 1 liter, pH7.2-7.4.
3, pig seminal fluid according to claim 1 is preserved liquid, it is characterized in that set of dispense ratio by weight is:
Glucose 37g/l; Bovine serum albumin 1.5g/1; Trisodium Citrate 3.7g/l; Ethylenediamine tetraacetic acid (EDTA) 3.7g/l; Sodium bicarbonate 1.2g/l; Penicillin 100 million international units; Streptomycin sulphate 100 million international units, adding distil water to 1 liter, pH7.2-7.4.
4, pig seminal fluid according to claim 1 is preserved liquid, it is characterized in that set of dispense ratio by weight is:
Glucose 37g/l; Bovine serum albumin 1g/l; Trisodium Citrate 3.7g/l; Ethylenediamine tetraacetic acid (EDTA) 3.7g/l; Sodium bicarbonate 1.2g/l; Repone K 0.4g/l; Penicillin 100 million international units; Streptomycin sulphate 100 million international units, adding distil water to 1 liter, pH7.2-7.4.
5, pig seminal fluid according to claim 1 is preserved liquid, it is characterized in that set of dispense ratio by weight is:
Glucose 60g/l; Bovine serum albumin 0.8g/l; Trisodium Citrate 6g/l; Ethylenediamine tetraacetic acid (EDTA) 3.7g/l; Sodium bicarbonate 1.2g/l; Repone K 0.4g/l; Penicillin 50 million international units; Streptomycin sulphate 50 million international units, adding distil water to 1 liter, pH7.2-7.4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410013364 CN1289661C (en) | 2004-06-25 | 2004-06-25 | Liquid for preserving pig semen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410013364 CN1289661C (en) | 2004-06-25 | 2004-06-25 | Liquid for preserving pig semen |
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| CN1712519A CN1712519A (en) | 2005-12-28 |
| CN1289661C true CN1289661C (en) | 2006-12-13 |
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| CN 200410013364 Expired - Fee Related CN1289661C (en) | 2004-06-25 | 2004-06-25 | Liquid for preserving pig semen |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200688B (en) * | 2007-10-30 | 2013-03-27 | 华坚青 | Boar semen freezing preservation tube and method thereof |
| CN101543208B (en) * | 2009-03-31 | 2012-05-30 | 华南农业大学 | Powder for pig sperm dilution and room temperature preservation and preparation method and application thereof |
| EP2443920B1 (en) | 2009-06-17 | 2016-12-21 | Hiroshima University | Sperm diluent solution and method for artificial insemination using same |
| CN101796945A (en) * | 2010-04-12 | 2010-08-11 | 西北农林科技大学 | Antifreeze used for freezing and preserving boar semens as well as preparation and application thereof |
| CN101856016B (en) * | 2010-06-19 | 2013-03-13 | 张尤嘉 | Diluent for preserving porcine semen at normal temperature |
| CN103380770B (en) * | 2013-06-27 | 2015-09-02 | 中国农业科学院特产研究所 | A kind of racoon dog dilution for preserving semen under normal temperature and semen collection method |
| CN103783034B (en) * | 2014-01-20 | 2015-10-21 | 广西大学 | One boar stem spermatogonium cryopreserving liquid and using method thereof |
| CN104770362B (en) * | 2015-03-12 | 2017-08-15 | 西北农林科技大学 | Deoxyschizandrin is used for the application for preparing pig semen preservation dilution |
| CN106508888A (en) * | 2016-10-26 | 2017-03-22 | 湖北省农业科学院畜牧兽医研究所 | Pig semen long-acting diluting powder and application thereof |
| CN107232184B (en) * | 2017-07-26 | 2021-02-05 | 西北农林科技大学 | Diluent for preserving semen of black pig in Guanzhong at normal temperature |
| CN108244095B (en) * | 2017-09-08 | 2018-11-02 | 东北农业大学 | One boar freezes smart defrosting dilution and its preparation method |
| CN111202052A (en) * | 2020-03-16 | 2020-05-29 | 厦门汇承药业有限公司 | Preparation method of pig semen diluent |
| CN112852720A (en) * | 2021-02-04 | 2021-05-28 | 西北农林科技大学 | Sheep X, Y sperm sorting solution, sorting planktonic system and sorting method |
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