CN103380770B - A kind of racoon dog dilution for preserving semen under normal temperature and semen collection method - Google Patents
A kind of racoon dog dilution for preserving semen under normal temperature and semen collection method Download PDFInfo
- Publication number
- CN103380770B CN103380770B CN201310274435.0A CN201310274435A CN103380770B CN 103380770 B CN103380770 B CN 103380770B CN 201310274435 A CN201310274435 A CN 201310274435A CN 103380770 B CN103380770 B CN 103380770B
- Authority
- CN
- China
- Prior art keywords
- racoon dog
- semen
- dilution
- dog
- racoon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of recoon dog dilution for preserving semen under normal temperature and semen collection method, it is characterized in that each component of dilution and suitable concentration thereof are: glucose 36-38g, sodium bicarbonate 1.20-1.30g, ethylenediamine tetra-acetic acid 1.20-1.30g, sodium citrate 5.50-6.30g, potassium chloride 0.65-0.85g, bovine serum albumin(BSA) 0.50-3.05g, acetylcysteine 0.05-0.30g, penicillin 0.50-1.20g, streptomycin 0.30-1.00g, lincomycin 0.05-1.50g, spectinomycin 0.01-0.05g, tri-distilled water is settled to 1000ml; Semen collection method key trains male racoon dog to make it the reflection of artificial semenpicking formation condition for dummy teaser for utilization female racoon dog that oestruses, and repeats simulation mother racoon dog stand replacement that 3-4 time utilization is placed with female racoon dog urine of oestrusing after the reflection of its formation condition female racoon dog that oestruses and can adopt to obtain male racoon dog seminal fluid smoothly.Utilize the present invention to adopt smoothly public racoon dog sperm dilution preserve after artificial insemination obtain offspring, by the popularization promoting racoon dog technology of artificial insemination, improve and support racoon dog industry economic benefit.
Description
Technical field
The present invention relates to breeding biological technical field, particularly relate to a kind of racoon dog dilution for preserving semen under normal temperature and semen collection method.
Background technology
Racoon dog is precious fur-bearing animal, and low with feeding cost, that crude feed tolerance, premunition are strong feature becomes one of large fur-bearing animal of China three, and its cultivation quantity increases year by year, and current China produces raccoon fur amount per year and ranks first in the world.Although racoon dog is very large in China's cultivation amount, but its breeding also adopts this friendship mode at present, this friendship needs the public racoon dog of a large amount of kinds to increase feeding cost, wasting manpower and material resources, and male racoon dog oestruses early than female racoon dog, often occurring planting in a large number public racoon dog in the breeding later stage cannot service condition, and racoon dog technology of artificial insemination can address these problems.
Racoon dog is because the time of raising and train is short, raising scale is little and the restriction of the factor such as species specificity difference, and the research data of domestic and international racoon dog semen collection and related fields is little.The animal artificial insemination such as current fox, dog are more ripe, about racoon dog artificial insemination due to the research and extension of semen collection problems affect.The research of current racoon dog Semen routine mainly concentrates in semen collection method, and the research of preserving special dilution about racoon dog semen at normal temperature is little, not yet knows that internet retrieval finds in China.
Summary of the invention
In order to improve the availability of the public racoon dog of breeding, reduce feeding and management cost.Accelerate racoon dog group improved speed, improve racoon dog fanning economics, the object of the invention is for racoon dog artificial insemination provides a kind of racoon dog dilution for preserving semen under normal temperature and semen collection method.Forefathers are being studied and is using for reference on the semen at normal temperature dilution formula of other animals and the basis of semen collection method, according to recoon dog sperm cell characteristics and Biological characteristics, with the important objective index such as sperm motility rate, survival index, acrosomal integrity, abnormal rate of preserving for foundation, develop a kind of racoon dog dilution for preserving semen under normal temperature formula and a kind of racoon dog semen collection method.
The formula of this dilution is: glucose 36-38g, sodium bicarbonate 1.20-1.30g, ethylenediamine tetra-acetic acid 1.20-1.30g, sodium citrate 5.50-6.30g, potassium chloride 0.65-0.85g, bovine serum albumin(BSA) 0.50-3.05g, N-acetyl-L-cysteine 0.05-0.30g, penicillin 0.50-1.20g, streptomycin 0.30-1.00g, lincomycin 0.05-1.50g, spectinomycin 0.01-0.05g, tri-distilled water is settled to 1000ml.Dilution adopts 0.22 μm of frit, and after sperm dilution, normal temperature storage temperature is 17 DEG C.
The key of semen collection method is that utilization female racoon dog that oestruses trains male racoon dog to make it the reflection of artificial semenpicking formation condition for dummy teaser, repeats to utilize after the reflection of its formation condition for 3-4 time the simulation mother racoon dog stand replacement being placed with female racoon dog urine of oestrusing female racoon dog that oestruses can adopt to obtain male racoon dog seminal fluid smoothly.
Compared with prior art, utilize the present invention to adopt smoothly public racoon dog seminal fluid carry out preservations and artificial insemination after dilution and also obtain offspring smoothly, practical semen collection technology and respond well semen diluent, the popularization of racoon dog technology of artificial insemination will be promoted to a great extent, improve and support racoon dog industry economic benefit.
Embodiment
1. the preparation of dilution: get glucose 37.538g, sodium bicarbonate 1.255g, ethylenediamine tetra-acetic acid 1.262g, sodium citrate 6.035g, potassium chloride 0.758g, bovine serum albumin(BSA) 1.052g, N-acetyl-L-cysteine 0.169g, penicillin 0.625g, streptomycin 0.505g, lincomycin 0.150g, spectinomycin 0.305g be dissolved in tri-distilled water, be settled in 1000ml volumetric flask, frozen with using or be sub-packed in aseptic bottle-20 DEG C after 0.22 μm of frit in time.
2. semen collection: female racoon dog that will oestrus is fixed on semen collection frame, is placed in snap ring by its neck and locked.Allow the female racoon dog of the male racoon dog mounting of semen collection, the public racoon dog in assistant Baoding.After cleaning public racoon dog external genital with warm suds, warm water washes down, and then soaks public racoon dog lower abdomen hair with 38-50 DEG C of 0.1%-0.2% bromogeramine soaking disinfection towel, is as the criterion not drip with water.First massage public racoon dog testis and perineum 15-60s during semen collection, excite sexual desires to public racoon dog semen collection signal.Thumb, forefinger are pinched in public racoon dog penis both sides by semen collection person, and middle finger is pinched at the penis outside of belly, and the shape that holds a pen to pinch in the middle part of penis and along the longitudinal strip pressure of penis and slip prepuce of penis, carries out friction stimulation to penis.Sliding speed 4-6 time/s during beginning, strip moves amplitude 5-7 centimetre.After telotism and bulbo cavernous are expanded, foreskin strip is continued the penis at strip pressure bulbo cavernous and rear portion to bulbo cavernous rear, strip pressure speed slows down, and strip per second presses 1 time, during strip pressure bulbo cavernous; Slightly firmly, strip pressing massage is till public racoon dog ejaculation so repeatedly, and another holds the smart cup of collection and collects seminal fluid rapidly.Or with another hand thumb and forefinger stimulating glans penis gently after cavernous body expands, hold the smart cup of collection with nameless and the centre of the palm and collect seminal fluid.Try not penis to pull to rear during semen collection between public racoon dog two back leg, front, side should be pulled to.Every semen collection in a day once, can be oestrused after repeating 3-4 formation condition reflection female racoon dog, adopt the female racoon dog stand of the simulation being placed with female racoon dog urine of oestrusing to get final product smooth extraction seminal fluid by after semen collection male racoon dog mounting simulating bench, utilize this method to coordinate the female racoon dog stand semen collection success rate of simulation to reach more than 80%.
3. smart for collection cup is put into 35 DEG C of water-baths, draw 20 μ l semen examination sperm viability and density with pipettor, get vigor more than 0.8 seminal fluid and dilute with this semen diluent isothermal preheated in advance, dilution is slowly injected seminal fluid, adds while shake up.The seminal fluid diluted can immediately for semen deposition, also can after slow cooling to room temperature 17 DEG C preserve semen deposition after 24-48h.
4. Semen routione Indexs measure: the mensuration of sperm viability adopts ocular estimate.From insulating box, take out normal temperature preserve seminal fluid, draw 30 μ l with pipettor and be placed on 37 DEG C of preheating slides, immediately at 200 times of optical microphotograph Microscopic observations after covered.Each sample random observation 3 visuals field, the number of sperm detecting visual field cathetus forward travel accounts for the ratio of total number of sperm as sperm viability, calculates sperm effective time-to-live and survival index.Adopt Nanjing to build up sperm rapid dyeing (quick improvement Pasteur method) kit, detect and preserve rate of teratosperm and acrosomal integrity, concrete outcome is in table 1.
5. artificial insemination: the seminal fluid applying the preservation of this example carries out artificial insemination to normal Wusuli Racoon Doy of oestrusing immediately and after 24h.Carry out pregnancy check after 25d, gestation mother is raised until healthy cub of giving a birth to obtain, artificial insemination conception rate more than 90%.
Table 1 dilution that the present embodiment makes preserves racoon dog semen quality testing result
Claims (1)
1. a recoon dog dilution for preserving semen under normal temperature, it is characterized in that each component of semen diluent and suitable concentration thereof are: glucose 37.538g, sodium bicarbonate 1.255g, ethylenediamine tetra-acetic acid 1.262g, sodium citrate 6.035g, potassium chloride 0.758g, bovine serum albumin(BSA) 1.052g, N-acetyl-L-cysteine 0.169g, penicillin 0.625g, streptomycin 0.505g, lincomycin 0.150g, spectinomycin 0.305g, tri-distilled water is settled to 1000ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310274435.0A CN103380770B (en) | 2013-06-27 | 2013-06-27 | A kind of racoon dog dilution for preserving semen under normal temperature and semen collection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310274435.0A CN103380770B (en) | 2013-06-27 | 2013-06-27 | A kind of racoon dog dilution for preserving semen under normal temperature and semen collection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103380770A CN103380770A (en) | 2013-11-06 |
| CN103380770B true CN103380770B (en) | 2015-09-02 |
Family
ID=49488862
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310274435.0A Expired - Fee Related CN103380770B (en) | 2013-06-27 | 2013-06-27 | A kind of racoon dog dilution for preserving semen under normal temperature and semen collection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103380770B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104663650A (en) * | 2015-03-02 | 2015-06-03 | 天津市宝坻区人民医院 | Sperm diluent preparing method |
| CN107802373A (en) * | 2017-10-30 | 2018-03-16 | 石阡裕和原生态农牧发展有限公司 | A kind of boar semen collection platform and its semen collection method |
| CN112852720A (en) * | 2021-02-04 | 2021-05-28 | 西北农林科技大学 | Sheep X, Y sperm sorting solution, sorting planktonic system and sorting method |
| CN115354036B (en) * | 2022-10-24 | 2023-03-24 | 北京纳捷诊断试剂有限公司 | Stabilizer of reverse transcriptase |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563359A (en) * | 2004-04-22 | 2005-01-12 | 上海交通大学 | Diluent for sperm of blue fox |
| CN1712519A (en) * | 2004-06-25 | 2005-12-28 | 华中农业大学 | Liquid for preserving pig semen |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007224004A (en) * | 2006-02-24 | 2007-09-06 | Yasushi Kuwabara | Diluting and preserving agent of semen for artificial insemination of ultrasmall pig |
-
2013
- 2013-06-27 CN CN201310274435.0A patent/CN103380770B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563359A (en) * | 2004-04-22 | 2005-01-12 | 上海交通大学 | Diluent for sperm of blue fox |
| CN1712519A (en) * | 2004-06-25 | 2005-12-28 | 华中农业大学 | Liquid for preserving pig semen |
Non-Patent Citations (6)
| Title |
|---|
| 对种公猪采精调教的体会;万全书等;《云南畜牧兽医》;20061231(第4期);15-16 * |
| 狐狸人工授精的研究-新鲜精液子宫内输精试验;孔庆松等;《野生动物》;19920828(第4期);32-34 * |
| 猪精液常温保存液;K.F.Weitze;《国外畜牧科技》;19901227(第6期);21-22 * |
| 貉按摩法采精技术的研究;韩欢胜等;《黑龙江畜牧兽医》;20090331(第5期);111-112 * |
| 貉精液冷冻配种技术的研究与效果;张旭秋等;《黑龙江畜牧兽医》;19991210(第12期);41 * |
| 貉精液采集技术的研究;韩欢胜;《东北林业大学硕士学位论文》;20080115;22、25、27 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103380770A (en) | 2013-11-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| O'Hara et al. | Effect of bovine blastocyst size at embryo transfer on day 7 on conceptus length on day 14: Can supplementary progesterone rescue small embryos? | |
| CN103380770B (en) | A kind of racoon dog dilution for preserving semen under normal temperature and semen collection method | |
| Robeck et al. | Deep intra-uterine artificial inseminations using cryopreserved spermatozoa in beluga (Delphinapterus leucas) | |
| Kendall et al. | Incidence and treatment of inadequate postovulatory progesterone concentrations in repeat breeder cows | |
| CN103271779A (en) | Artificial fertilization method for breeding geese | |
| Szostak et al. | The influence of the breed and age on the libido of insemination boars | |
| Benham et al. | Production of embryos and a live offspring using post mortem reproductive material from bison (Bison bison bison) originating in Yellowstone National Park, USA | |
| Navanukraw et al. | Estrous and ovulatory responses following cervical artificial insemination in Thai-native goats given a new or once-used controlled internal drug release with human chorionic gonadotropin | |
| Netto et al. | Use of two cloprostenol administrations 11.5 days apart efficiently synchronizes oestrus in photostimulated multiparous dairy goats in the non‐breeding season | |
| Jafariahangari et al. | The effect of pre-natal maternal environment on live weight, reproductive and semen characteristics in ram lambs | |
| Ombelet et al. | History of human artificial insemination | |
| Metodiev et al. | Short term progestagen treatment for estrus synchronization at nulliparous ewes from the Synthetic Population Bulgarian Milk | |
| CN110438068B (en) | Method for improving in-vitro fertilization embryo development rate of river buffalo | |
| Escrivão et al. | Effects of 48-hour calf withdrawal on conception rates of Bos indicus cows and calf weaning weights in extensive production systems | |
| Mat et al. | Preliminary study on influence of parity and body condition score (BCS) on estrus response and conception rate of Saanen-cross by estrus synchronization with CIDR | |
| CN203458494U (en) | Raccoon dog semen collection stand | |
| JP5087718B2 (en) | Method of collecting semen from experimental animals and its artificial insemination | |
| Meuffels-Barkas et al. | Comparative reproduction of the female horse, elephant and rhinoceros: implications for advancing assisted reproductive technologies | |
| Jeong et al. | Influence of somatic cell donor breed on reproductive performance and comparison of prenatal growth in cloned canines | |
| Hufana-Duran et al. | Full-term delivery of river buffalo calves (2n= 50) from in vitro-derived vitrified embryos by swamp buffalo recipients (2n= 48) | |
| Forshey et al. | Embryo production by superovulation and dual siring in alpacas (Vicugna pacos) | |
| Bezjian et al. | Characterization and cryopreservation of semen from endangered markhor goats (Capra falconeri heptneri) with evaluation of reproductive seasonality | |
| Aller et al. | Birth of live llama (Lama glama) derived from embryo transfer storage at 5° C for 24 h | |
| CHANDEL | EVALUATION OF INTRA-VAGINAL PROGESTERONE RELEASING INSERT IN REPEAT BREEDER COWS | |
| Qi et al. | Effect of Ovum Pick-Up Frequency on Quality of Buffalo Oocytes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150902 Termination date: 20170627 |