CN106508888A - Pig semen long-acting diluting powder and application thereof - Google Patents
Pig semen long-acting diluting powder and application thereof Download PDFInfo
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- CN106508888A CN106508888A CN201610954233.4A CN201610954233A CN106508888A CN 106508888 A CN106508888 A CN 106508888A CN 201610954233 A CN201610954233 A CN 201610954233A CN 106508888 A CN106508888 A CN 106508888A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
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Abstract
The invention belongs to the technical field of pig propagation and particularly relates to pig semen long-acting diluting powder and an application thereof. The pig semen long-acting diluting powder is prepared from the following components in parts by weight: 27-30g of glucose, 6-7g of trisodium citrate dehydrate, 2-3g of citric acid monohydrate, 2-3g of ethylenediamine tetraacetic acid, 0.5-1g of sodium bicarbonate, 0.3-0.6g of potassium chloride, 5-6g of tris(hydroxymethyl)metyl aminomethane, 3-4g of bovine serum albumin and 0.1-0.3g of antibiotic enrofloxacin. An optimized formula comprises 27.69g of glucose, 6.03g of trisodium citrate dehydrate, 2.52g of citric acid monohydrate, 2.03g of ethylenediamine tetraacetic acid, 0.83g of sodium bicarbonate, 0.46g of potassium chloride, 5.08g of tris(hydroxymethyl)metyl aminomethane, 3.69g of bovine serum albumin and 0.18g of antibiotic enrofloxacin. Semen can be kept as long as 11-13 days. The pig semen long-acting diluting powder can be applied to the auxiliary propagation of pigs.
Description
Technical field
The invention belongs to pig reproduction technique field, and in particular to a kind of long-acting dilution powder of pig semen and its application.
Background technology
Artificial Insemination Technology of Swine be gathered boar semen and quality inspection and dilution carried out to seminal fluid with manual implement after, then
Seminal fluid is injected in heat sow genital tract, to replace a kind of breeding method of boar natural mating, 80 years 20th century with apparatus
Generation worldwide rapid popularization, at present in the use area such as wider Europe and North America, popularity rate more than 80% is
The science of carrying out pig, the important means for realizing Swine Production modernization, it has the following advantages：(1) boar is saved, and is reduced per head
The access times of boar, overcome time and area differentiation, easily can breed in good time；(2) human and material resources, financial resources are saved, is carried
High economic benefit, reduces transmission of disease；(3) breeding rate is improved, as the instruments such as microscope, Density Measuring Instrument can be utilized to sperm
Quality is monitored, and effectively increases conception rate, and can adjust the poor boar of sperm quality in time；(4) increase heredity
The utilization rate of the boar of function admirable, expands breeding coverage rate, is conducive to breed improvement and union breeding.
In the artificial insemination work for carrying out pig, it is necessary to prepare the dilution of boar semen, to dilute preservation.
Application number 201310478237.6 discloses a kind of diluting boar semen agent, and application number 201010133910.9 is disclosed
A kind of porcine semen at normal temperature preserves diluent and its prepares and dilution process, preserves the seminal fluid time only 3 days；Application number
The 201410164618.1 antiviral semen diluent of pig, preservation seminal fluid time only have 4 days.
The disclosure of the invention of the Application No. 201410527131.5 antiviral long-acting semen dilution formula of liquid of a boar and system
Preparation Method, the time for preserving seminal fluid are 5-7 days, and its defect is that the polyvinyl alcohol in filling a prescription in the preparation is needed in heating electromagnetic
It is dissolved on agitator in the distilled water of 150ml 30-50 DEG C, stirs 20-40 minutes, prepares trouble.
A kind of long-acting diluting boar semen with the disclosure of the invention of the closely related application number 201210238501.4 of the present invention
Powder and preparation method thereof, the holding time of pig semen is 10-12 days, but dry chicken egg-yolk powder preparation method therein is complicated, system
During standby, contaminated probability is big, needs corresponding drying equipment, and quality guarantee period is short.
So develop and preserving pig semen time length, preparing easy boar semen dilution powder to technology of artificial insemination with weight
Want meaning.
The content of the invention
It is an object of the invention to overcome the defect of prior art, there is provided a kind of long-acting dilution powder of pig semen and its application,
The present invention draw materials easily, prepare it is easy, be easy to storage, can using the Semen routine time of the long-acting semen dilution powder of pig of the present invention
Up to 11-13 days.
It is a further object of the invention to provide a kind of application process of the long-acting dilution powder of pig semen.
Technical scheme is as described below：
A kind of long-acting dilution powder of pig semen, it is formed by the component mixed preparing of following weight portion：Glucose 27-30g,
Citrate dihydrate trisodium 6-7g, Citric Acid Mono 2-3g, ethylenediamine tetra-acetic acid 2-3g, sodium acid carbonate 0.5-1g, potassium chloride 0.3-
0.6g, trishydroxymethylaminomethane 5-6g, bovine serum albumin(BSA) 3-4g and antibiotic Enrofloxacin 0.1-0.3g.
Used as optimization formula, the long-acting dilution powder of pig semen of the present invention, it is by the component mixed preparing of following weight portion
Form：Glucose 27.69g, citrate dihydrate trisodium 6.03g, Citric Acid Mono 2.52g, ethylenediamine tetra-acetic acid 2.03g, carbonic acid
Hydrogen sodium 0.83g, potassium chloride 0.46g, trishydroxymethylaminomethane 5.08g, bovine serum albumin(BSA) 3.69g and antibiotic Enrofloxacin
0.18g。
A kind of application process of the long-acting dilution powder of pig semen, comprises the steps：Collection boar semen, measure are permeated
It is 7.04 ± 0.05 to press as 301.25 ± 14.52mmol/kg, pH value, according to the preparation purpose of the long-acting dilution powder of pig semen -- it is right
Sperm is harmless, can provide sperm nutrition, and pH scopes are adapted to sperm survival, and osmotic pressure is appropriate, and raw material is easy to get, configures simple, long-acting
Preserve, carry out the screening operation of component, screening each component for obtaining is：Glucose, citrate dihydrate trisodium
(C6H5Na307.2H2O), Citric Acid Mono, ethylenediamine tetra-acetic acid (EDTA), sodium acid carbonate (NaHCo3), potassium chloride (Kcl), three
Hydroxymethyl aminomethane (TRIS), bovine serum albumin(BSA) (BSA) and antibiotic Enrofloxacin.
According to the boar semen osmotic pressure and pH value and seminar that obtain pre- formula result of the test in advance is determined, by chlorine
Change potassium, bovine serum albumin(BSA) and Enrofloxacin as Main change factor, graded levels are 3, are tried using L9 (34) form
Test, 3 repetitions (seminal fluid of 3 boars), screening formula.Then the further optimization filled a prescription, adjusts physical and chemical parameter, to ooze
Thoroughly based on pressure, osmotic pressure gradient test, S1 (265mmol/kg), S2 (277mmol/kg), S3 (288mmol/kg), S4 are carried out
(309mmol/kg), S5 (318mmol/kg), observes sperm motility, tests through osmotic pressure gradient, finds osmotic pressure in 300-
310 or so is optimal, will fill a prescription by optimal osmotic pressure and further optimizes, and each test period is 13 days or so, until absolutely mostly
Number semen sample vigor stops detection after being reduced to 0.6, and then analysis obtains optimal formula combination, and the formula after optimization is dilute
Seminal fluid is released, is preserved 11-13 days, sperm motility is 0.60.
The invention has the beneficial effects as follows：
During natural mating, a boar can undertake 25 sows every year carries out the breeding load of this friendship, and adopts and manually award
Essence, a boar can match somebody with somebody more than 250 sows in 1 year, and compared with this friendship, its advantage can be to save the feeding of boar for artificial insemination
Support amount.Artificial insemination is carried out using long-acting dilution powder dilution pig semen of the invention, it is possible to reduce the sterilization in artificial insemination procedures
With the number of times with dilution, dilution seminal fluid, pig semen saves to use at any time within the time of a fixed length, energy-saving
Thing.With the pig semen of the long-acting dilution powder dilution of the pig semen of present invention preparation under the constant temperature that temperature is 17 DEG C, Ke Yibao
Deposit 11-13 days.
Specific embodiment
The present invention is described in further details below by embodiment, embodiment is only used for illustrating the present invention, does not limit
The scope of the present invention processed；Embodiment is raw materials used using the pure rank of analysis, and all steps are carried out in better purifying space, better purifying space
Purity requirements be 300,000 grades.
Embodiment 1
A kind of long-acting dilution powder of pig semen, it is formed by following components in parts by weight mixed preparing：Glucose 30.00g,
Citrate dihydrate trisodium 6.53g, Citric Acid Mono 2.73g, ethylenediamine tetra-acetic acid 2.20g, sodium acid carbonate 0.90g, potassium chloride
0.50g, trishydroxymethylaminomethane 5.50g, bovine serum albumin(BSA) 4.00g and antibiotic Enrofloxacin 0.20g.
Test method：
(1) embodiment raw material is sealed after weighing, vacuum packaging, is obtained dilution powder using analysis pure rank, and temperature is 2-8
DEG C, relative humidity be less than 50% under conditions of preserve.
(2) when using dilution powder, with the redistilled water stirring for being heated to 35-37 DEG C, it is well mixed, constant volume is arrived
1000ml, obtains semen diluent, is used for the dilution of pig semen after 1.5 hours.
(3), after pig semen collection, smell, the normal pig semen of color are first used sperm concentration instrument detection density, then pre-
Microscopy sperm motility under 100 power microscopes on hot 37 DEG C of slides, vigor calculate dilution with formula up to more than 0.9 grade of pig semen
Number：Weight × density × vigor ÷ 30=numbers (30 refer to the effective sperm count of every part of seminal fluid 3,000,000,000).
(4) no more than 1 DEG C of semen diluent and the pig semen temperature difference, and pig is made by the temperature for adjusting semen diluent
The temperature of seminal fluid and semen diluent is as close as, it is impossible to reverse operating.
(5) semen diluent is poured slowly in pig semen along chamber wall when diluting pig semen, drop should not be big, with not
Spume and be defined, be then shaken gently for or with sterilization glass bar stirring, both is softly mixed；Calculate by volume, such as make 1：2-4
Times dilution for many times when, should first make 1：1-1.5 times low power dilution, after half a minute by remaining semen diluent along container
Wall is slowly added to, and can not pour pig semen in semen diluent in turn.All dilution operations will be in room temperature 23-25 DEG C ring
Under border, complete in 30min.
(6) pig semen after dilution is slowly loaded in the seminal fluid bottle or semen bag of cleaning, with whole seminal fluid bottle or seminal fluid
Bag volume calculations, air content 6-7%, closed seminal fluid bottle or semen bag, are reduced sperm and are contacted with oxygen, the generation of Inhibit sperm
Thank, extend the holding time of seminal fluid, (can also use clean dry pure under 23-25 DEG C of room temperature after 1 hour of lucifuge slow cooling
After the big towel of cotton wraps up 2 layers) preservation in 17 DEG C of insulating boxs is positioned over, the slow shake seminal fluid every 8 hours, it is to avoid sperm deposition is made
Into injures and deaths.A sperm motility was detected every 24 hours.
(7) indoor no smoking, the cosmetics that female operator can not use smell dense, the thermometer contacted with seminal fluid,
Glass bar, diluted cup, 37 DEG C of preheatings of dilution water.To avoid in the transportation of pig semen sunlight irradiation, the vibrations that avoid jolting,
The temperature difference is avoided to rise and fall sharply and quickly.Using special constant temperature transport case or bubble chamber, summer puts frozen water bag, and winter puts cotton towel, protects
The temperature inside the box is held for 15-20 DEG C.
The implementation result of the present embodiment is shown in Table 1.
1 boar semen of table, 17 DEG C of constant temperature preserve vigour changes statistics
Embodiment 2
A kind of long-acting dilution powder optimum formula of pig semen is formed by the component mixed preparing of following weight portion：Glucose
27.69g, citrate dihydrate trisodium 6.03g, Citric Acid Mono 2.52g, ethylenediamine tetra-acetic acid 2.03g, sodium acid carbonate 0.83g, chlorine
Change potassium 0.46g, trishydroxymethylaminomethane 5.08g, bovine serum albumin(BSA) 3.69g and antibiotic Enrofloxacin 0.18g.
Test method：
(1) embodiment raw material is sealed after weighing, vacuum packaging, is obtained dilution powder using analysis pure rank, and temperature is 2-8
DEG C, relative humidity be less than 50% under conditions of preserve.
(2) when using dilution powder, with the redistilled water stirring for being heated to 35-37 DEG C, it is well mixed, constant volume is arrived
1000ml, obtains semen diluent, is used for the dilution of pig semen after 1.5 hours.
(3), after pig semen collection, smell, the normal pig semen of color are first used sperm concentration instrument detection density, then pre-
Microscopy sperm motility under 100 power microscopes on hot 37 DEG C of slides, vigor calculate dilution with formula up to more than 0.9 grade of pig semen
Number：Weight × density × vigor ÷ 30=numbers (30 refer to the effective sperm count of every part of seminal fluid 3,000,000,000).
(4) no more than 1 DEG C of semen diluent and the pig semen temperature difference, and pig is made by the temperature for adjusting semen diluent
The temperature of seminal fluid and semen diluent is as close as, it is impossible to reverse operating.
(5) semen diluent is poured slowly in pig semen along chamber wall when diluting pig semen, drop should not be big, with not
Spume and be defined, be then shaken gently for or with sterilization glass bar stirring, both is softly mixed；Calculate by volume, such as make 1：2-4
Times dilution for many times when, should first make 1：1-1.5 times low power dilution, after half a minute by remaining semen diluent along container
Wall is slowly added to, and can not pour pig semen in semen diluent in turn.All dilution operations will be in room temperature 23-25 DEG C ring
Under border, complete in 30min.
(6) pig semen after dilution is slowly loaded in the seminal fluid bottle or semen bag of cleaning, with whole seminal fluid bottle or seminal fluid
Bag volume calculations, air content 6-7%, closed seminal fluid bottle or semen bag, are reduced sperm and are contacted with oxygen, the generation of Inhibit sperm
Thank, extend the holding time of seminal fluid, (can also use clean dry pure under 23-25 DEG C of room temperature after 1 hour of lucifuge slow cooling
After the big towel of cotton wraps up 2 layers) preservation in 17 DEG C of insulating boxs is positioned over, the slow shake seminal fluid every 8 hours, it is to avoid sperm deposition is made
Into injures and deaths.A sperm motility was detected every 24 hours.
(7) indoor no smoking, the cosmetics that female operator can not use smell dense, the thermometer contacted with seminal fluid,
Glass bar, diluted cup, 37 DEG C of preheatings of dilution water.To avoid in the transportation of pig semen sunlight irradiation, the vibrations that avoid jolting,
The temperature difference is avoided to rise and fall sharply and quickly.Using special constant temperature transport case or bubble chamber, summer puts frozen water bag, and winter puts cotton towel, protects
The temperature inside the box is held for 15-20 DEG C.
The implementation result of the present embodiment is shown in Table 1.
With the pig semen after the long-acting dilution powder formula dilution of pig semen after optimization, can preserve 11-13 days, sperm motility is
0.60.Test shows that the sperm of straight ahead motion accounts for total sperm count in 60-95%, and the pregnancy rate of artificial insemination sow is without aobvious
Write difference.
Claims (3)
1. the long-acting dilution powder of a kind of pig semen, it is characterised in that mixed by the component of following weight portion：Glucose 27-
30g, citrate dihydrate trisodium 6-7g, Citric Acid Mono 2-3g, ethylenediamine tetra-acetic acid 2-3g, sodium acid carbonate 0.5-1g, potassium chloride
0.3-0.6g, trishydroxymethylaminomethane 5-6g, bovine serum albumin(BSA) 3-4g and antibiotic Enrofloxacin 0.1-0.3g.
2. the long-acting dilution powder of a kind of pig semen, it is characterised in that mixed by the component of following weight portion：Glucose
27.69g, citrate dihydrate trisodium 6.03g, Citric Acid Mono 2.52g, ethylenediamine tetra-acetic acid 2.03g, sodium acid carbonate 0.83g, chlorine
Change potassium 0.46g, trishydroxymethylaminomethane 5.08g, bovine serum albumin(BSA) 3.69g and antibiotic Enrofloxacin 0.18g.
3. application of the long-acting dilution powder of pig semen described in claim 1 or 2 in pig supplementary reproduction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610954233.4A CN106508888A (en) | 2016-10-26 | 2016-10-26 | Pig semen long-acting diluting powder and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610954233.4A CN106508888A (en) | 2016-10-26 | 2016-10-26 | Pig semen long-acting diluting powder and application thereof |
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| Publication Number | Publication Date |
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| CN106508888A true CN106508888A (en) | 2017-03-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610954233.4A Pending CN106508888A (en) | 2016-10-26 | 2016-10-26 | Pig semen long-acting diluting powder and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113545336A (en) * | 2021-07-19 | 2021-10-26 | 杨凌职业技术学院 | Pig semen purifying agent |
| CN115868476A (en) * | 2022-09-14 | 2023-03-31 | 广州金晨生物技术有限公司 | Long-acting boar semen dilution powder and preparation method thereof |
| CN116210678A (en) * | 2023-02-23 | 2023-06-06 | 安徽农业大学 | Normal temperature preserving preparation for pig semen and its use method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1712519A (en) * | 2004-06-25 | 2005-12-28 | 华中农业大学 | Liquid for preserving pig semen |
| CN103299987A (en) * | 2013-05-29 | 2013-09-18 | 西北农林科技大学 | Diluent for normal temperature preservation of boar semens and preparation method thereof |
-
2016
- 2016-10-26 CN CN201610954233.4A patent/CN106508888A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1712519A (en) * | 2004-06-25 | 2005-12-28 | 华中农业大学 | Liquid for preserving pig semen |
| CN103299987A (en) * | 2013-05-29 | 2013-09-18 | 西北农林科技大学 | Diluent for normal temperature preservation of boar semens and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 茆达干 等: "不同稀释液对猪精液常温保存效果的研究", 《畜牧兽医杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113545336A (en) * | 2021-07-19 | 2021-10-26 | 杨凌职业技术学院 | Pig semen purifying agent |
| CN115868476A (en) * | 2022-09-14 | 2023-03-31 | 广州金晨生物技术有限公司 | Long-acting boar semen dilution powder and preparation method thereof |
| CN116210678A (en) * | 2023-02-23 | 2023-06-06 | 安徽农业大学 | Normal temperature preserving preparation for pig semen and its use method |
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Application publication date: 20170322 |