CN113545336A - Pig semen purifying agent - Google Patents
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- CN113545336A CN113545336A CN202110815619.8A CN202110815619A CN113545336A CN 113545336 A CN113545336 A CN 113545336A CN 202110815619 A CN202110815619 A CN 202110815619A CN 113545336 A CN113545336 A CN 113545336A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
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Abstract
The invention relates to the field of pig industry, in particular to a pig semen purifying agent which is prepared from the following raw materials: 15-25 g of glucose, 6-16 g of trisodium citrate, 1-5 g of ethylene diamine tetraacetic acid disodium, 0.5-3.5 g of sodium bicarbonate, 0.5-1.5 g of polyvinyl alcohol, 2-11 g of tricarboxymethylaminomethane, 1-8 g of citric acid, 0.1-0.3 g of cysteine, 0.05-0.15ml of ribavirin injection, 0.05-0.15ml of enrofloxacin injection, 0.05-0.15ml of radix bupleuri injection and 1200ml of deionized water 800-. The technology has the advantages that the broad-spectrum antibacterial agent and the Chinese herbal medicine extracting solution are added in the semen diluting process, the semen is invisible and toxic, bacteria breeding in the semen collecting and diluting processes are relieved and purified, the semen quality is optimized, and the breeding performance is improved.
Description
Technical Field
The invention relates to the field of pig industry, in particular to a pig semen purifying agent.
Background
The breeding link of the modern pig industry adopts an artificial insemination technology, namely, the collected original semen is used for artificial insemination after being diluted and subpackaged. At present, the boar purification mainly adopts breeding boar screening, and boars with clinical expression diseases can not be used for hybridization. The semen is invisible and toxic, and bacteria breeding in the semen collecting and diluting process cannot be purified, so that the breeding performance is influenced.
Disclosure of Invention
The invention aims to provide a pig semen purifying agent to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a pig semen purifying agent is prepared from the following raw materials: 15-25 g of glucose, 6-16 g of trisodium citrate, 1-5 g of ethylene diamine tetraacetic acid disodium, 0.5-3.5 g of sodium bicarbonate, 0.5-1.5 g of polyvinyl alcohol, 2-11 g of tricarboxymethylaminomethane, 1-8 g of citric acid, 0.1-0.3 g of cysteine, 0.05-0.15ml of ribavirin injection, 0.05-0.15ml of enrofloxacin injection, 0.05-0.15ml of radix bupleuri injection and 1200ml of deionized water 800-.
As a still further scheme of the invention: the feed is prepared from the following raw materials: 17-23 g of glucose, 8-14 g of trisodium citrate, 2-4 g of ethylene diamine tetraacetic acid, 1-2.5 g of sodium bicarbonate, 0.7-1.3 g of polyvinyl alcohol, 4-9 g of tricarboxymethylaminomethane, 3-6 g of citric acid, 0.15-0.25 g of cysteine, 0.07-0.13ml of ribavirin injection, 0.07-0.13ml of enrofloxacin injection, 0.07-0.13ml of radix bupleuri injection and 900-1100ml of deionized water.
As a still further scheme of the invention: the feed is prepared from the following raw materials: 20 g of glucose, 11.65 g of trisodium citrate, 2.35 g of ethylene diamine tetraacetic acid disodium salt, 1.75 g of sodium bicarbonate, 1g of polyvinyl alcohol, 5.65 g of tricarboxymethylaminomethane, 4.1 g of citric acid, 0.20 g of cysteine, 0.1ml of ribavirin injection, 0.1ml of enrofloxacin injection, 0.1ml of radix bupleuri injection and 1000ml of deionized water.
As a still further scheme of the invention: the content of ribavirin in the ribavirin injection is 50 mg/ml.
As a still further scheme of the invention: the enrofloxacin injection has the enrofloxacin content of 25 mg/ml.
A preparation method of a pig semen purifying agent comprises the following steps:
(1) dissolving polyvinyl alcohol in 100-200ml of deionized water at 30-50 ℃ on a heating electromagnetic stirrer to obtain a polyvinyl alcohol solution;
(2) dissolving other components in 400-600ml of cold deionized water, adding a polyvinyl alcohol solution, and finally adding the rest deionized water to obtain a finished product.
As a still further scheme of the invention: the stirring time in the step (1) is 25-35 min.
Compared with the prior art, the invention has the beneficial effects that: the technology has the advantages that the broad-spectrum antibacterial agent and the Chinese herbal medicine extracting solution are added in the semen diluting process, the semen is invisible and toxic, bacteria breeding in the semen collecting and diluting processes are relieved and purified, the semen quality is optimized, and the breeding performance is improved.
Detailed Description
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The present invention will be described in detail with reference to examples.
Example one
A pig semen purifying agent is prepared from the following raw materials: 15 g of glucose, 6 g of trisodium citrate, 1g of ethylene diamine tetraacetic acid disodium salt, 0.5 g of sodium bicarbonate, 0.5 g of polyvinyl alcohol, 2 g of tricarboxymethylaminomethane, 1g of citric acid, 0.1g of cysteine, 0.05ml of ribavirin injection, 0.05ml of enrofloxacin injection, 0.05ml of radix bupleuri injection and 800ml of deionized water.
The content of ribavirin in the ribavirin injection is 50 mg/ml.
The enrofloxacin injection has the enrofloxacin content of 25 mg/ml.
A preparation method of a pig semen purifying agent comprises the following steps:
(1) dissolving polyethylene ethanol in 100ml of deionized water at 30 ℃ on a heating electromagnetic stirrer to obtain a polyethylene ethanol solution;
(2) dissolving the other components in 400ml of cold deionized water, adding a polyethylene ethanol solution, and finally adding the rest deionized water to obtain a finished product.
The stirring time in the step (1) is 25 min.
Example two
A pig semen purifying agent is prepared from the following raw materials: 25 g of glucose, 16 g of trisodium citrate, 5 g of ethylene diamine tetraacetic acid disodium salt, 3.5 g of sodium bicarbonate, 1.5 g of polyvinyl alcohol, 11 g of tricarboxymethylaminomethane, 8 g of citric acid, 0.3 g of cysteine, 0.15ml of ribavirin injection, 0.15ml of enrofloxacin injection, 0.15ml of radix bupleuri injection and 1200ml of deionized water.
The content of ribavirin in the ribavirin injection is 50 mg/ml.
The enrofloxacin injection has the enrofloxacin content of 25 mg/ml.
A preparation method of a pig semen purifying agent comprises the following steps:
(1) dissolving polyvinyl alcohol in 200ml of deionized water at 50 ℃ on a heating electromagnetic stirrer to obtain a polyvinyl alcohol solution;
(2) dissolving other components in 600ml of cold deionized water, adding a polyethylene ethanol solution, and finally adding the rest deionized water to obtain a finished product.
The stirring time in the step (1) is 35 min.
EXAMPLE III
A pig semen purifying agent is prepared from the following raw materials: 20 g of glucose, 11.65 g of trisodium citrate, 2.35 g of ethylene diamine tetraacetic acid disodium salt, 1.75 g of sodium bicarbonate, 1g of polyvinyl alcohol, 5.65 g of tricarboxymethylaminomethane, 4.1 g of citric acid, 0.20 g of cysteine, 0.1ml of ribavirin injection, 0.1ml of enrofloxacin injection, 0.1ml of radix bupleuri injection and 1000ml of deionized water.
The content of ribavirin in the ribavirin injection is 50 mg/ml.
The enrofloxacin injection has the enrofloxacin content of 25 mg/ml.
A preparation method of a pig semen purifying agent comprises the following steps:
(1) dissolving polyethylene ethanol in 150ml of deionized water at 40 ℃ on a heating electromagnetic stirrer to obtain a polyethylene ethanol solution;
(2) dissolving the other components in 500ml of cold deionized water, adding a polyethylene ethanol solution, and finally adding the rest deionized water to obtain a finished product.
The stirring time in the step (1) is 30 min.
Enrofloxacin is a broad-spectrum bactericidal drug and has special effects on mycoplasma. Has antibacterial effect on Escherichia coli, Klebsiella, Salmonella, Proteus, Pseudomonas aeruginosa, Haemophilus, Pasteurella multocida, hemolytic Pasteurella, Staphylococcus aureus, and Streptococcus.
Enrofloxacin can be used as animal medicine, has long half-life period in animal body, good tissue distribution, belongs to broad-spectrum bacteriostat, has bacteriostasis effect on gram-positive bacteria, gram-negative bacteria and mildews, and is used for controlling vibriosis and colibacillosis diseases of cultured fishes.
Experimental example 1:
screening of antibacterial drugs in semen dilution
In the experiment, the antibacterial effect of the antibacterial agent on the semen culture medium is observed by adding the antibacterial agent with different concentrations into the semen diluent, so that a basis is provided for the selection and application of the antibacterial agent in the pig semen diluent.
TABLE 1 comparison of the antibacterial Effect of drugs in the porcine semen dilution
Note: "-" indicates that the total number of bacteria is 0; "+" indicates the total number of bacteria ≦ 50; "+ +" indicates the total number of bacteria is 50-100; "+ + + +" indicates a total number of bacteria ≧ 100.
The data in Table 1 show that streptomycin sulfate and gentamicin are present at a concentration of 10-2When the bacterial colony number of the plate is 0, all bacteria in the tested semen can be inhibited. PenicillinSodium at a concentration of 10-3When the number of bacterial colonies on the plate is 0, all bacteria in the test semen can be inhibited. Enrofloxacin at a concentration of 10-4When the number of bacterial colonies on the plate is 0, all bacteria in the test semen can be inhibited. The results of antibacterial effect comparison tests of adding different antibacterial drugs into the boar semen diluent show that the enrofloxacin can achieve the effect of inhibiting all bacteria in the tested semen at the lowest concentration, namely the enrofloxacin has the best effect of inhibiting the bacteria in the boar semen diluent.
Experimental example 2:
effect of antibacterial Agents on semen preservation time
Diluting with the dilution solution of antibiotics of different concentration groups, preserving sperm at constant temperature, and checking sperm motility every 24 h.
The basic semen diluent formula is added with antibacterial drugs (penicillin, streptomycin and enrofloxacin) which are divided into 10-2～10-5Several dilution gradients. And (3) placing the diluent in a water bath, heating to 37 ℃ to enable the diluent to be equal to the temperature of the just-collected original semen, wherein the semen diluent is mixed with the collected original semen 1: 1 mixing, diluting, storing in a special constant temperature refrigerator for semen, and detecting the sperm motility rate every 24 hours. The sperm survival time refers to the preservation time from the constant temperature preservation after the semen is diluted in vitro to the sperm motility reduction of 0.6.
The experimental data in table 2 show that: penicillin and streptomycin are added into semen diluent, after 72 hours, the sperm activity of 4 dilution ratio groups is more than 0.6, and semen has the matching capability. After 96h, the concentration of group 2 i.e. penicillin streptomycin was 10-3The sperm motility is kept at 0.6, while the sperm motility rates of other 3 observation groups are all reduced to be below 0.6, and the sperm lose the utilization value of artificial insemination due to low motility rate. When the preservation time extends to 96h, the sperm motility rate of the group 2 is 0.6, and the sperm is preserved in vitro at constant temperature for 4 d. After 5d in vitro preservation, the sperm motility rate in all the streptomycin dilution test groups is reduced to be below 0.4. Indicating that streptomycin is present at a concentration of 10-3Preserving semen in vitro at constant temperature for 4 days.
When the concentration gradient of penicillin is 10-3When the dilution is required, 50 million units of the dilution is added into each 1000ml of the dilutionThe penicillin has the longest sperm in-vitro preservation time under the concentration gradient, and the diluent with the concentration gradient larger or smaller than the concentration gradient can obviously influence the effective preservation time of the sperm, namely the concentration of the antibacterial drug in the diluent influences the preservation time of the sperm during the constant-temperature preservation of the sperm in vitro. When the concentration of the antibacterial agent is too high, the sperm is easily damaged to shorten the preservation time, and when the concentration of the antibacterial agent is too low, the bacteria cannot be prevented from expanding and breeding in the semen due to poor bacteriostatic effect, so that the preservation time outside the semen is short.
TABLE 2 Effect of antimicrobial on sperm preservation time
Preserving sperm in the enrofloxacin diluents with different concentration gradients at constant temperature in vitro, wherein the sperm motility of the enrofloxacin diluents with different concentration gradients is over 0.6 after 96 hours, namely the effective preservation time of the enrofloxacin diluents sperm for maintaining insemination capability is over 4 days, wherein 10 enrofloxacin is used-4The semen diluted by the concentration gradient is effectively preserved for 5 days at constant temperature in vitro. The test result shows that the effect of enrofloxacin in preserving semen is superior to that of streptomycin diluent. 10-2And 10-3Group due to the excessive concentration of the drug, the semen preservation effect is poor, and the sperm may be damaged by the high concentration of the antibacterial drug 10-5The quality of the semen preservation effect of the group is poor, and the concentration of the medicine is low, so that the bacteriostatic effect cannot be achieved.
When the concentration gradient of the enrofloxacin is 10-4In the process, 0.1g of analytically pure enrofloxacin antibacterial is added into every 1000ml of diluent, the in-vitro preservation time of the sperms is longest under the concentration gradient, the effective preservation time of the sperms can be obviously influenced by the enrofloxacin diluent with the concentration gradient larger or smaller than the concentration gradient, namely the preservation time of the sperms is influenced by the concentration of the enrofloxacin antibacterial in the diluent during the in-vitro constant-temperature preservation of the sperms. When the concentration of the antibacterial agent is too high, spermatogenesis is easy to occurThe storage time is shortened due to damage, and when the concentration of the antibacterial agent is too low, the in-vitro storage time of the sperms is short due to the fact that bacteria cannot be prevented from expanding and propagating in the semen because of poor antibacterial effect.
Experimental example 3
Effect of antiviral Chinese herbal medicine on sperm survival time
2 antiviral chemical drugs, 5 antiviral Chinese medicinal preparations are added into the basic diluent screened in the research to form different antiviral diluent combinations, the different antiviral diluent combinations are compared with the basic semen diluent, and the influence of sperm motility after the semen is diluted by the antiviral semen diluent is measured. Provides scientific basis for the normal-temperature preservation of the boar semen and the research of the antiviral semen. All assays were run in duplicate.
TABLE 3 Effect of antiviral drugs on sperm survival time (h)
Note: the different shoulder marks in the table represent significant differences (p > 0.05).
As shown in the test results in Table 3, the concentration gradient (drug/diluent) of 2 western antiviral drugs of moroxydine and acyclovir is larger than 10-4In the above, the in vitro preservation time of the sperms is less than 1d, which indicates that 2 western medicines cause the sperms to die within 24 hours under the normal temperature preservation condition due to overlarge concentration, namely 2 antiviral western medicines have large stimulation and damage effects on the sperms and are not beneficial to maintaining the effective preservation time and the activity of the sperms.
When the drug concentration gradient is 10-5When the medicine is taken (0.01 g/1000ml of diluent of concentrated freeze-dried traditional Chinese medicine), the traditional Chinese medicines of radix bupleuri, ribavirin, honeysuckle, Lianzhou and radix isatidis are all obviously higher than 2 chemical antiviral medicines (moroxydine and acyclovir) of other antiviral medicines, and the medicine concentration gradient (medicine diluent) is 10 because the medicine has great effect on sperm damage-5In time, the sperm have low vitality and can not reach the technical standard of artificial insemination, and the sperm are eliminated. The effect of 5 antiviral traditional Chinese medicines on sperm preservation and activity is less than that of western medicines, 10-5Can be effectively preserved at a certain concentrationSemen 4d above.
The diluent of the bupleurum and ribavirin test group is used for preserving sperms in vitro, and the result shows that the sperms have the best tolerance to 2 drug combinations, and the effective semen preservation time can reach 6 days. The ribavirin virus saliva component is used as an antiviral chemical drug, has small damage to sperms, meets the requirements of artificial insemination technical standards on sperm motility, can effectively improve semen preservation time and sperm preservation quality, and can be used as a semen diluent antiviral drug to be combined with Chinese medicament radix bupleuri.
Experimental example 4
Killing effect of antiviral drug on Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
Virus 100TCID50(PRRSV, maintenance of 2% New born calf serum) and an equal volume dilution of 10-5The drug samples are mixed and acted for 2 hours at 37 ℃. Digesting and passaging Marc145 cells according to a conventional method, adjusting the cell concentration, inoculating 100ul of cell suspension into each hole, culturing until the cells grow to be full of a monolayer, removing the culture medium, washing with PBS for three times, inoculating a mixed solution of virus and traditional Chinese medicine extract after 2 hours of action, repeating for 4 holes at 100 ul/hole, and additionally arranging a cell control and a virus control. Incubation at 37 ℃ with 5% carbon dioxide was continued until the virus control showed CPE. After the supernatant was aspirated, washed 2 to 3 times with PBS, 20. mu.l of MTT solution (5mg/ml) was added thereto, and after 4 hours of incubation, the culture medium was removed, 150. mu.l of dimethyl sulfoxide was added to each well, and the mixture was shaken on a shaker at a low speed for 15 minutes to dissolve the formazan crystal. Zero setting wells (medium, MTT, DMSO. Absorbance of each well was measured at OD570 nm.
TABLE 4 Experimental results of the killing effect of antiviral drugs on PRRSV
| Drug sample | A570Value of | Inhibition ratio (%) |
| Ribavirin | 0.253±0.008 | 72 |
| Honeysuckle | 0.249±0.010 | 61 |
| Forsythia fruit | 0.258±0.007 | 66 |
| Radix Isatidis | 0.255±0.021 | 67 |
| Radix bupleuri and ribavirin | 0.269±0.011 | 87 |
| Cell control group | 0.273±0.003 | 1 |
| Virus control group | 0.203±0.011 | 0 |
Note: inhibition rate (drug treatment group a)570Value-viral control group A570Value)/(cell control A570Value-viral control group A570Value) 100%.
The data in table 4 show that the inhibition rates of ribavirin, honeysuckle, forsythia, isatis root and the bupleurum and ribavirin combined drug on the PRRSV virus are 72%, 61%, 66%, 67% and 87%, respectively. The combination of the bupleurum and the ribavirin obviously enhances the inhibition effect of the PRRSV virus.
Experimental example 5
Test of effect of pig semen purifying agent on production performance of breeding sows
The method is carried out in two breeding sow houses of the same variety, and the breeding sows are respectively set as an experimental group and a control group. Artificial insemination was performed three times after the same estrus in 2 houses using artificial insemination breeding mode, the first afternoon, the second morning and the second afternoon, respectively. And counting the conception rate after 21 days, observing the health degree of pregnant sows in the gestation period, counting the survival rate of piglets after 21-day parturition and weaning, and comparing the medicine use cost in the two housing feeding management processes in the whole period.
The test result shows that the fertility performances of the artificial insemination breeding used in the two houses, such as conception rate, litter average litter size, and the like, are different. The results are shown in Table 5. As can be seen from the table 5, the artificial insemination effect of the pig semen purifying agent is better than that of a control group, the conception rate of the sows is 5.21 percent higher than that of control common diluent, meanwhile, the drug cost is reduced by 4.66 ten thousand yuan in the breeding cycle, and the pig semen purifying agent can effectively improve the reproductive performance of the sows. The healthy rate of weaned healthy piglets is 2.52% higher than that of the control group, which indicates that the boar semen purifying agent can effectively improve the health level of swinery. The effect of gradually purifying the swinery is achieved.
Influence of artificial insemination of pig semen purificant on conception rate and litter size of long and white pigs
Product pilot test summary: the pig semen purifying agent is practically applied to pig raising enterprises, and shows that the pig semen purifying agent is effectively preserved for 7-12 days at constant temperature in vitro, the conception rate is improved by 7%, and the health rate of swineries is improved by 30%. Compared with the application effect of the pig semen purifying agent and the diluted powder, the pig raising comprehensive benefit is improved. The pig semen purifying agent can obtain good practical application effect when being applied to artificial insemination of pigs.
The pig semen purifying agent can be directly used for semen alleviation purification after pre-warming, and links such as double distilled water preparation, magnetic stirrer stirring and the like are omitted; the sterile environment is also ensured by ultra-high temperature sterilization in the manufacturing process flow. The pig semen purifying agent product is added with broad-spectrum antibiotics and Chinese herbal medicine extract, can purify invisible semen with toxicity, and can breed bacteria in the processes of collecting and diluting semen. Meanwhile, the product is convenient to operate, sterile and efficient, the breeding performance of the sows is improved, and the swinery is purified.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (7)
1. The pig semen purifying agent is characterized by being prepared from the following raw materials: 15-25 g of glucose, 6-16 g of trisodium citrate, 1-5 g of ethylene diamine tetraacetic acid disodium, 0.5-3.5 g of sodium bicarbonate, 0.5-1.5 g of polyvinyl alcohol, 2-11 g of tricarboxymethylaminomethane, 1-8 g of citric acid, 0.1-0.3 g of cysteine, 0.05-0.15ml of ribavirin injection, 0.05-0.15ml of enrofloxacin injection, 0.05-0.15ml of radix bupleuri injection and 1200ml of deionized water 800-.
2. The pig semen purifying agent as claimed in claim 1, which is prepared from the following raw materials: 17-23 g of glucose, 8-14 g of trisodium citrate, 2-4 g of ethylene diamine tetraacetic acid, 1-2.5 g of sodium bicarbonate, 0.7-1.3 g of polyvinyl alcohol, 4-9 g of tricarboxymethylaminomethane, 3-6 g of citric acid, 0.15-0.25 g of cysteine, 0.07-0.13ml of ribavirin injection, 0.07-0.13ml of enrofloxacin injection, 0.07-0.13ml of radix bupleuri injection and 900-1100ml of deionized water.
3. The pig semen purifying agent as claimed in claim 1, which is prepared from the following raw materials: 20 g of glucose, 11.65 g of trisodium citrate, 2.35 g of ethylene diamine tetraacetic acid disodium salt, 1.75 g of sodium bicarbonate, 1g of polyvinyl alcohol, 5.65 g of tricarboxymethylaminomethane, 4.1 g of citric acid, 0.20 g of cysteine, 0.1ml of ribavirin injection, 0.1ml of enrofloxacin injection, 0.1ml of radix bupleuri injection and 1000ml of deionized water.
4. The pig semen purification agent as claimed in any one of claims 1 to 3, wherein the content of ribavirin in the ribavirin injection is 50 mg/ml.
5. The boar semen purifying agent according to any one of the claims 1 to 3, wherein the content of enrofloxacin in the enrofloxacin injection is 25 mg/ml.
6. The method for preparing a pig semen purifying agent as claimed in any one of claims 1 to 5, which comprises the following steps:
(1) dissolving polyvinyl alcohol in 100-200ml of deionized water at 30-50 ℃ on a heating electromagnetic stirrer to obtain a polyvinyl alcohol solution;
(2) dissolving other components in 400-600ml of cold deionized water, adding a polyvinyl alcohol solution, and finally adding the rest deionized water to obtain a finished product.
7. The method for preparing the pig semen purifying agent as claimed in claim 6, wherein the stirring time in the step (1) is 25-35 min.
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Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1349797A (en) * | 2000-09-11 | 2002-05-22 | Imv技术公司 | Attenuant for preserving pig's sperm |
| CN1814749A (en) * | 2005-02-04 | 2006-08-09 | 谢来安 | Instant pork-essence liquor diluting solution |
| UA84411U (en) * | 2013-03-07 | 2013-10-25 | Інститут Біології Тварин Уаан | Ecosperm b medium for dilution and storage of boars semen |
| CN104322483A (en) * | 2014-10-09 | 2015-02-04 | 广东中农联生物制药有限公司 | Boar antiviral long-acting semen diluent formula and preparation method |
| CN105010304A (en) * | 2014-04-23 | 2015-11-04 | 马乃祥 | Prescription composition of pig antiviral semen diluent |
| WO2015193265A1 (en) * | 2014-06-16 | 2015-12-23 | Università degli Studi di Parma | Composition for extenders for the long-term conservation of animal seminal material |
| CN106259306A (en) * | 2016-08-03 | 2017-01-04 | 江苏农牧科技职业学院 | A kind of pig seminal fluid cryopreservation method |
| CN106417259A (en) * | 2016-10-26 | 2017-02-22 | 湖北省农业科学院畜牧兽医研究所 | Boar seminal fluid long-acting diluting powder and application thereof |
| CN106508888A (en) * | 2016-10-26 | 2017-03-22 | 湖北省农业科学院畜牧兽医研究所 | Pig semen long-acting diluting powder and application thereof |
| CN107372460A (en) * | 2017-07-13 | 2017-11-24 | 漳州傲农现代农业开发有限公司 | A kind of porcine semen at normal temperature of anti-oxidation stress preserves diluent, preserves liquid and application |
| CN107912422A (en) * | 2017-11-21 | 2018-04-17 | 广东中农联生物制药有限公司 | A kind of dilution for pig semen and preparation method thereof |
| KR20180109321A (en) * | 2017-03-27 | 2018-10-08 | 농업회사법인 주식회사 대영육가공 | The formula for pig foot |
| CN109010534A (en) * | 2018-11-01 | 2018-12-18 | 衡阳市武顺循环农牧绿色生态科技有限公司 | Prevent and treat drug and preparation method thereof of the boar without smart dead essence |
| CN111202052A (en) * | 2020-03-16 | 2020-05-29 | 厦门汇承药业有限公司 | Preparation method of pig semen diluent |
-
2021
- 2021-07-19 CN CN202110815619.8A patent/CN113545336A/en active Pending
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1349797A (en) * | 2000-09-11 | 2002-05-22 | Imv技术公司 | Attenuant for preserving pig's sperm |
| CN1814749A (en) * | 2005-02-04 | 2006-08-09 | 谢来安 | Instant pork-essence liquor diluting solution |
| UA84411U (en) * | 2013-03-07 | 2013-10-25 | Інститут Біології Тварин Уаан | Ecosperm b medium for dilution and storage of boars semen |
| CN105010304A (en) * | 2014-04-23 | 2015-11-04 | 马乃祥 | Prescription composition of pig antiviral semen diluent |
| WO2015193265A1 (en) * | 2014-06-16 | 2015-12-23 | Università degli Studi di Parma | Composition for extenders for the long-term conservation of animal seminal material |
| CN104322483A (en) * | 2014-10-09 | 2015-02-04 | 广东中农联生物制药有限公司 | Boar antiviral long-acting semen diluent formula and preparation method |
| CN106259306A (en) * | 2016-08-03 | 2017-01-04 | 江苏农牧科技职业学院 | A kind of pig seminal fluid cryopreservation method |
| CN106417259A (en) * | 2016-10-26 | 2017-02-22 | 湖北省农业科学院畜牧兽医研究所 | Boar seminal fluid long-acting diluting powder and application thereof |
| CN106508888A (en) * | 2016-10-26 | 2017-03-22 | 湖北省农业科学院畜牧兽医研究所 | Pig semen long-acting diluting powder and application thereof |
| KR20180109321A (en) * | 2017-03-27 | 2018-10-08 | 농업회사법인 주식회사 대영육가공 | The formula for pig foot |
| CN107372460A (en) * | 2017-07-13 | 2017-11-24 | 漳州傲农现代农业开发有限公司 | A kind of porcine semen at normal temperature of anti-oxidation stress preserves diluent, preserves liquid and application |
| CN107912422A (en) * | 2017-11-21 | 2018-04-17 | 广东中农联生物制药有限公司 | A kind of dilution for pig semen and preparation method thereof |
| CN109010534A (en) * | 2018-11-01 | 2018-12-18 | 衡阳市武顺循环农牧绿色生态科技有限公司 | Prevent and treat drug and preparation method thereof of the boar without smart dead essence |
| CN111202052A (en) * | 2020-03-16 | 2020-05-29 | 厦门汇承药业有限公司 | Preparation method of pig semen diluent |
Non-Patent Citations (4)
| Title |
|---|
| MANPOONG等: "Efficacy of potassium permanganate and turmeric as antimicrobial agents on the bacterial load and quality of boar semen during preservation at 15 degrees C", 《VETERINARSKI ARHIV》 * |
| 万遂如: "高度重视猪的免疫抑制性传染病的防治", 《养猪》 * |
| 张金龙等: "抗生素对猪精液常温保存效果的影响", 《黑龙江动物繁殖》 * |
| 马乃祥: "猪高效精液稀释液研究与应用效果评价", 《中国博士学位论文全文数据库 (农业科技辑)》 * |
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