CN111202052A - Preparation method of pig semen diluent - Google Patents
Preparation method of pig semen diluent Download PDFInfo
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- CN111202052A CN111202052A CN202010183124.3A CN202010183124A CN111202052A CN 111202052 A CN111202052 A CN 111202052A CN 202010183124 A CN202010183124 A CN 202010183124A CN 111202052 A CN111202052 A CN 111202052A
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- diluent
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
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- Zoology (AREA)
- Environmental Sciences (AREA)
- Biophysics (AREA)
- Physiology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a preparation method of a pig semen diluent, and relates to the field of livestock breeding. The boar semen diluent comprises the following components in parts by weight: 30-40 parts of nutrient, 15-25 parts of buffer solution, 1-3 parts of potassium chloride, 1-2 parts of ethylene diamine tetraacetic acid, 1-3 parts of protective agent, 0.1-0.3 part of vitamin E, 2-5 parts of enrofloxacin, 0.5-0.8 part of amphotericin B and 1000 parts of solution 1400, wherein the nutrient is one of glucose, fructose, lactose and mannose, preferably glucose, the buffer solution is a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, the ratio of the sodium dihydrogen phosphate to the disodium hydrogen phosphate is 1:1, the protective agent is a mixture of bovine serum albumin and glycerol, the ratio of the bovine serum albumin to the glycerol is 1: 3-4. Through reasonable material selection and preparation mode for can provide same suitable preservation environment for boar semen, and then this diluent can greatly increased when long to the save of boar semen, the work of later stage insemination of being convenient for goes on.
Description
Technical Field
The invention relates to the technical field of livestock breeding, in particular to a preparation method of a pig semen diluent.
Background
Nowadays, animal husbandry has become a great important industry, with the continuous development and progress of society, people start scientific breeding, in order to optimize and improve the breeds of pigs in breeding, semen of boars is transmitted into the bodies of sows in a conveying mode, so that the sows are pregnant, the mode is also called artificial insemination, the technology greatly pushes the vigorous development of the animal husbandry industry in China, and great contribution is made to the improvement of excellent breeds of the pigs.
At present, the artificial insemination between the boar semen needs to use the diluent, but most of the diluents are powder diluents and some liquid diluents, but the diluents have short preservation time when the boar semen is preserved, so the boar semen is easy to lose efficacy, and certain defects exist.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a preparation method of a boar semen diluent, which solves the problems that the existing diluent has short preservation time when the boar semen is preserved, the boar semen is easy to lose efficacy, and certain defects exist in use.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a preparation method of a boar semen diluent comprises the following steps of: 30-40 parts of nutrient, 15-25 parts of buffer solution, 1-3 parts of potassium chloride, 1-2 parts of ethylene diamine tetraacetic acid, 1-3 parts of protective agent, 0.1-0.3 part of vitamin E, 2-5 parts of enrofloxacin, 0.5-0.8 part of amphotericin B and 1400 parts of solution.
Preferably, the nutrient is one of glucose, fructose, lactose and mannose, and is preferably glucose.
Preferably, the buffer solution is a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, and the ratio of the sodium dihydrogen phosphate to the disodium hydrogen phosphate is 1: 1.
Preferably, the protective agent is a mixture of bovine serum albumin and glycerol, and the ratio of the bovine serum albumin to the glycerol is 1: 3-4.
Preferably, the solution is one of distilled water, double distilled water and triple distilled water.
A method for preparing a diluent of pig semen is characterized by comprising the following steps: the preparation method comprises the following preparation steps:
s1, selecting nutrients, buffer solution, potassium chloride, Ethylene Diamine Tetraacetic Acid (EDTA) ethanol, protective solution, vitamin E, enrofloxacin and amphotericin in a quantitative ratio for sterilization treatment to obtain sterilized raw materials;
s2, mixing, stirring and dissolving the sterilized nutrient, potassium chloride, ethylene diamine tetraacetic acid, protective solution, vitamin E, enrofloxacin and amphotericin with the solution to obtain a diluent;
s3, injecting the buffer solution into the obtained diluent for multiple times slowly and continuously stirring, measuring the pH of the diluent in real time in the injection process, and adjusting the pH to 6.5-6.9 to obtain the final diluent;
and S4, bagging, sealing and storing the obtained diluent for later use.
Preferably, the stirring speed in S2 and S3 is 200-500r/min, and the stirring time in S2 is not less than 1 hour.
Preferably, in S4, the bag needs to be sterilized before being sealed.
(III) advantageous effects
The invention provides a preparation method of a boar semen diluent. The method has the following beneficial effects:
1. through reasonable material selection and preparation mode for can provide same suitable preservation environment for boar semen, and then this diluent can greatly increased when long to the save of boar semen, the work of later stage insemination of being convenient for goes on.
2. Common raw materials are selected for preparation, so that the production process is more convenient, and the use cost in the production process can be reduced.
Detailed Description
The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
the embodiment of the invention provides a preparation method of a boar semen diluent, wherein the boar semen diluent comprises the following components in parts by weight: 30 parts of nutrient, 15 parts of buffer solution, 1 part of potassium chloride, 1 part of ethylene diamine tetraacetic acid, 1 part of protective agent, 0.1 part of vitamin E, 2 parts of enrofloxacin, 0.5 part of amphotericin B and 1000 parts of solution.
Wherein the nutrient is one of glucose, fructose, lactose and mannose, preferably glucose.
The buffer solution is a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, wherein the ratio of the sodium dihydrogen phosphate to the disodium hydrogen phosphate is 1: 1.
Wherein the protective agent is a mixture of bovine serum albumin and glycerol, wherein the ratio of the bovine serum albumin to the glycerol is 1: 3-4.
Wherein the solution is one of distilled water, double distilled water and triple distilled water.
A method for preparing a diluent of pig semen is characterized by comprising the following steps: the preparation method comprises the following preparation steps:
s1, selecting nutrients, buffer solution, potassium chloride, Ethylene Diamine Tetraacetic Acid (EDTA) ethanol, protective solution, vitamin E, enrofloxacin and amphotericin in a quantitative ratio for sterilization treatment to obtain sterilized raw materials;
s2, mixing, stirring and dissolving the sterilized nutrient, potassium chloride, ethylene diamine tetraacetic acid, protective solution, vitamin E, enrofloxacin and amphotericin with the solution to obtain a diluent;
s3, injecting the buffer solution into the obtained diluent for multiple times slowly and continuously stirring, measuring the pH of the diluent in real time in the injection process, and adjusting the pH to 6.5-6.9 to obtain the final diluent;
and S4, bagging, sealing and storing the obtained diluent for later use.
Wherein the stirring speed in S2 and S3 is 200-500r/min, and the stirring time in S2 is not less than 1 hour.
Wherein in S4, the bag needs to be sterilized before being sealed.
Example two:
the embodiment of the invention provides a preparation method of a boar semen diluent, wherein the boar semen diluent comprises the following components in parts by weight: 35 parts of nutrient, 20 parts of buffer solution, 2 parts of potassium chloride, 1.5 parts of ethylene diamine tetraacetic acid, 2 parts of protective agent, 0.2 part of vitamin E, 3 parts of enrofloxacin, 0.6 part of amphotericin B and 1200 parts of solution.
Wherein the nutrient is one of glucose, fructose, lactose and mannose, preferably glucose.
The buffer solution is a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, wherein the ratio of the sodium dihydrogen phosphate to the disodium hydrogen phosphate is 1: 1.
Wherein the protective agent is a mixture of bovine serum albumin and glycerol, wherein the ratio of the bovine serum albumin to the glycerol is 1: 3-4.
Wherein the solution is one of distilled water, double distilled water and triple distilled water.
A method for preparing a diluent of pig semen is characterized by comprising the following steps: the preparation method comprises the following preparation steps:
s1, selecting nutrients, buffer solution, potassium chloride, Ethylene Diamine Tetraacetic Acid (EDTA) ethanol, protective solution, vitamin E, enrofloxacin and amphotericin in a quantitative ratio for sterilization treatment to obtain sterilized raw materials;
s2, mixing, stirring and dissolving the sterilized nutrient, potassium chloride, ethylene diamine tetraacetic acid, protective solution, vitamin E, enrofloxacin and amphotericin with the solution to obtain a diluent;
s3, injecting the buffer solution into the obtained diluent for multiple times slowly and continuously stirring, measuring the pH of the diluent in real time in the injection process, and adjusting the pH to 6.5-6.9 to obtain the final diluent;
and S4, bagging, sealing and storing the obtained diluent for later use.
Wherein the stirring speed in S2 and S3 is 200-500r/min, and the stirring time in S2 is not less than 1 hour.
Wherein in S4, the bag needs to be sterilized before being sealed.
Example three:
the embodiment of the invention provides a preparation method of a boar semen diluent, wherein the boar semen diluent comprises the following components in parts by weight: 40 parts of nutrient, 25 parts of buffer solution, 3 parts of potassium chloride, 2 parts of ethylene diamine tetraacetic acid, 13 parts of protective agent, 0.3 part of vitamin E, 5 parts of enrofloxacin, 0.8 part of amphotericin B and 1400 parts of solution.
Wherein the nutrient is one of glucose, fructose, lactose and mannose, preferably glucose.
The buffer solution is a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, wherein the ratio of the sodium dihydrogen phosphate to the disodium hydrogen phosphate is 1: 1.
Wherein the protective agent is a mixture of bovine serum albumin and glycerol, wherein the ratio of the bovine serum albumin to the glycerol is 1: 3-4.
Wherein the solution is one of distilled water, double distilled water and triple distilled water.
A method for preparing a diluent of pig semen is characterized by comprising the following steps: the preparation method comprises the following preparation steps:
s1, selecting nutrients, buffer solution, potassium chloride, Ethylene Diamine Tetraacetic Acid (EDTA) ethanol, protective solution, vitamin E, enrofloxacin and amphotericin in a quantitative ratio for sterilization treatment to obtain sterilized raw materials;
s2, mixing, stirring and dissolving the sterilized nutrient, potassium chloride, ethylene diamine tetraacetic acid, protective solution, vitamin E, enrofloxacin and amphotericin with the solution to obtain a diluent;
s3, injecting the buffer solution into the obtained diluent for multiple times slowly and continuously stirring, measuring the pH of the diluent in real time in the injection process, and adjusting the pH to 6.5-6.9 to obtain the final diluent;
and S4, bagging, sealing and storing the obtained diluent for later use.
Wherein the stirring speed in S2 and S3 is 200-500r/min, and the stirring time in S2 is not less than 1 hour.
Wherein in S4, the bag needs to be sterilized before being sealed.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A method for preparing a diluent of pig semen is characterized by comprising the following steps: the boar semen diluent comprises the following components in parts by weight: 30-40 parts of nutrient, 15-25 parts of buffer solution, 1-3 parts of potassium chloride, 1-2 parts of ethylene diamine tetraacetic acid, 1-3 parts of protective agent, 0.1-0.3 part of vitamin E, 2-5 parts of enrofloxacin, 0.5-0.8 part of amphotericin B and 1400 parts of solution.
2. The method of claim 1, wherein the diluent is selected from the group consisting of: the nutrient is one of glucose, fructose, lactose and mannose, and is preferably glucose.
3. The method of claim 1, wherein the diluent is selected from the group consisting of: the buffer solution is a mixture of sodium dihydrogen phosphate and disodium hydrogen phosphate, and the ratio of the sodium dihydrogen phosphate to the disodium hydrogen phosphate is 1: 1.
4. The method of claim 1, wherein the diluent is selected from the group consisting of: the protective agent is a mixture of bovine serum albumin and glycerol, and the ratio of the bovine serum albumin to the glycerol is 1: 3-4.
5. The method of claim 1, wherein the diluent is selected from the group consisting of: the solution is one of distilled water, double distilled water and triple distilled water.
6. A method for preparing a diluent of pig semen is characterized by comprising the following steps: the preparation method comprises the following preparation steps:
s1, selecting nutrients, buffer solution, potassium chloride, Ethylene Diamine Tetraacetic Acid (EDTA) ethanol, protective solution, vitamin E, enrofloxacin and amphotericin in a quantitative ratio for sterilization treatment to obtain sterilized raw materials;
s2, mixing, stirring and dissolving the sterilized nutrient, potassium chloride, ethylene diamine tetraacetic acid, protective solution, vitamin E, enrofloxacin and amphotericin with the solution to obtain a diluent;
s3, injecting the buffer solution into the obtained diluent for multiple times slowly and continuously stirring, measuring the pH of the diluent in real time in the injection process, and adjusting the pH to 6.5-6.9 to obtain the final diluent;
and S4, bagging, sealing and storing the obtained diluent for later use.
7. The method of claim 6, wherein the diluent is selected from the group consisting of: the stirring speed in S2 and S3 is 200-500r/min, and the stirring time in S2 is not less than 1 hour.
8. The method of claim 6, wherein the diluent is selected from the group consisting of: in S4, the bag needs to be sterilized before being sealed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202010183124.3A CN111202052A (en) | 2020-03-16 | 2020-03-16 | Preparation method of pig semen diluent |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010183124.3A CN111202052A (en) | 2020-03-16 | 2020-03-16 | Preparation method of pig semen diluent |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113545336A (en) * | 2021-07-19 | 2021-10-26 | 杨凌职业技术学院 | Pig semen purifying agent |
Citations (4)
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|---|---|---|---|---|
| CN1712519A (en) * | 2004-06-25 | 2005-12-28 | 华中农业大学 | Liquid for preserving pig semen |
| CN101856016A (en) * | 2010-06-19 | 2010-10-13 | 张尤嘉 | Diluent for preserving porcine semen at normal temperature |
| KR101457526B1 (en) * | 2013-06-15 | 2014-11-03 | (주)나비바이오텍 | Diluent Compound for Improving Pig Sperm Preservation Ability |
| CN107372460A (en) * | 2017-07-13 | 2017-11-24 | 漳州傲农现代农业开发有限公司 | A kind of porcine semen at normal temperature of anti-oxidation stress preserves diluent, preserves liquid and application |
-
2020
- 2020-03-16 CN CN202010183124.3A patent/CN111202052A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1712519A (en) * | 2004-06-25 | 2005-12-28 | 华中农业大学 | Liquid for preserving pig semen |
| CN101856016A (en) * | 2010-06-19 | 2010-10-13 | 张尤嘉 | Diluent for preserving porcine semen at normal temperature |
| KR101457526B1 (en) * | 2013-06-15 | 2014-11-03 | (주)나비바이오텍 | Diluent Compound for Improving Pig Sperm Preservation Ability |
| CN107372460A (en) * | 2017-07-13 | 2017-11-24 | 漳州傲农现代农业开发有限公司 | A kind of porcine semen at normal temperature of anti-oxidation stress preserves diluent, preserves liquid and application |
Non-Patent Citations (1)
| Title |
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| 王志清: "《家畜繁殖和人工授精》", 31 July 1987, 安徽科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113545336A (en) * | 2021-07-19 | 2021-10-26 | 杨凌职业技术学院 | Pig semen purifying agent |
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Application publication date: 20200529 |
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