CN102687715A - Cold-water instant pig seminal fluid dilution powder formula and manufacturing process and application method - Google Patents
Cold-water instant pig seminal fluid dilution powder formula and manufacturing process and application method Download PDFInfo
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- CN102687715A CN102687715A CN2011100762016A CN201110076201A CN102687715A CN 102687715 A CN102687715 A CN 102687715A CN 2011100762016 A CN2011100762016 A CN 2011100762016A CN 201110076201 A CN201110076201 A CN 201110076201A CN 102687715 A CN102687715 A CN 102687715A
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Abstract
The invention provides a cold-water instant pig seminal fluid dilution powder formula, a manufacturing process and an application method, and is characterized in that each dosage of the formula comprises the following components: 36.0-40.0 g of glucose, 0.8-2.0 g of sodium bicarbonate, 6.0-8.0 g of trisodium citrate dihydrate, 1.0-3.0 g of potassium chloride, 1.0-3.0 g of citric acid monohydrate, 1.0-3.0 g of disodium ethylenediamine tetraacetic acid, 0.2-1.0 g of penicillin of 800,000 units, and 1.0-3.0 g of Tris (tris(hydroxymethyl)metyl aminomethane). The formula has easily-available materials, simple preparation, easy transport and storage, convenient usage, low cost, and long preservation time for seminal fluid; during application, only a certain amount of secondary distilled water is added, and dissolution is realized with cold water; the formula is suitable for dilution of pig seminal fluid which is preserved at a constant temperature of 17 DEG C, can reduce seminal fluid production cost and increase economic benefits.
Description
Technical field
The invention belongs to the swine artificial insemination technical field, be specifically related to a kind of can be in cold water rapidly-soluble dilution powder prescription and manufacture craft and the method for using that is used to preserve the pig seminal fluid.
Background technology
Along with the needs of the continuous development of China's pig industry and boar associating breeding work, the popularizing rapidly of swine artificial insemination technology, the swine artificial insemination station of different scales has become the raise pigs important component part of industry of China.The swine artificial insemination station must make full use of outstanding stock boar, brings into play its boar advantage, just can reach resource-sharing truly, and the holding time of boar semen, preservation effect are the key factors that success or failure are used in artificial insemination.Therefore, develop easy to use, preserve long and effective, cost of seminal fluid time and significantly be lower than existing commercial sperm dilution powder, significant as far as technology of artificial insemination.
At present, common thinner had liquid and two kinds of powder of solid particles states during swine artificial insemination was promoted.Wherein the liquid diluent processing technology complicated, be not easy to transportation and store.The difficult dissolving that the thinner of powder of solid particles state has needs to use magnetic stirrer or heating hydrotropy in the production, preparation time is long, and stabilization time is long, causes puzzlement to actual production.Simultaneously, the dilution powder that has like bovine serum albumin, HEPES (hydroxyethyl piperazine second thiosulfonic acid) etc., directly causes cost to rise because of containing the composition that some cost an arm and a leg, are difficult for purchase in the prescription, is unfavorable for large-scale promotion application.
Based on above factor, develop the diluting boar semen powder that relatively is fit to large scale of pig farm and small-sized Swine Production person use and be of great importance.
Summary of the invention
The purpose of this invention is to provide a kind of cold water instant dissolving type diluting boar semen powder prescription; Draw materials easily, preparation is easy, be easy to transportation and storage, cost is low, the seminal fluid holding time long, easy to use, only need add a certain amount of redistilled water during use and just can use, do not need winter to heat in advance yet and prepare used redistilled water; Thereby improve the utilization ratio of breeding boar; Quicken breed improvement, reduce the number of animals raised of boar, save the feeding and management expense; Reduce the production cost of seminal fluid, increase economic efficiency.
Another object of the present invention provides the manufacture craft of said diluting boar semen powder.
The method for using that also has a purpose to provide said diluting boar semen powder of the present invention.
Embodiment
The object of the invention is realized through following technical scheme:
A kind of boar semen dilution powder prescription is provided, and concrete composition is following:
Every part of finished product adds 1000 gram redistilled waters.
The preparation method is following:
Above-mentioned each composition is all selected for use and is analyzed pure level chemicals, pulverizes and cross 40 mesh sieves respectively, and accurately above-mentioned each composition of weighing fully mixes, and reaches uniformity, packs by every part 50 gram.
The operation room temperature requirement is below 20 ℃ in above weighing, pulverizing, mixing, the packaging process, and relative moisture is below 50%.
Storage, the method for using of the dilution powder of this prescription are following:
(1) finished product need be kept at cool place, dry place, guarantee temperature below 20 ℃, relative moisture is below 50%.
(2) per 50 gram finished products add 1000 gram redistilled waters, stir or shake for several times, can dissolve rapidly, promptly can be used for diluting the pig seminal fluid after 30 minutes.
The temperature that must regulate dilution according to the temperature of seminal fluid when (3) diluting seminal fluid, the two temperature difference must not be above 1 ℃.
(4) this dilution can be used for the low power dilution; Also can be used for dilution for many times; Extension rate is confirmed (according to the relevant regulations requirement of boar normal temperature seminal fluid standard GB 23238-2009, the sperm concentration of dilution back seminal fluid cathetus forward travel should more than 0.31 hundred million) according to the density of former seminal fluid.Should dilution slowly be poured in the seminal fluid with the glass bar drainage or along chamber wall during the dilution seminal fluid, can not conversely seminal fluid be poured in the dilution.
(5) slowly pack in clean the seminal fluid bottle or semen bag through the seminal fluid after the dilution, sealing, at room temperature lucifuge leaves standstill the insulating box of putting into 17 ℃ after 1 hour and preserves.Whenever slowly shook up seminal fluid, avoid the sperm deposition to cause injures and deaths at a distance from 8 hours.
The suitable formula components content of the present invention is:
Add 1000 gram redistilled waters.
Preserve experiment according to boar semen; Dilution powder with 50 these prescriptions of gram adds 1000 gram redistilled waters, after stirring or rocking evenly, is used for sperm dilution after 30 minutes; Seminal fluid after the dilution is packed in seminal fluid bottle or the semen bag; After lucifuge under 22～25 ℃ of room temperatures was slowly lowered the temperature 1 hour, use clean dry towel parcel to be positioned in 17 ℃ of insulating boxs and preserve, whenever slowly shook up once at a distance from 8 hours.Whenever detected a sperm viability at a distance from 24 hours.Slowly shake up seminal fluid before the detection; Draw 25 microlitre seminal fluid with pipettor and drop in (slide need carry out preheating in advance on 37 ℃ thermostatic electrothermal plate) on the slide; Add a cover cover glass; On 37 ℃ thermostatic electrothermal plate,,, observe 3 visuals field for every with microscope (200 times～400 times) observation vigor through preheating in 30 seconds.Each seminal fluid sample extraction three times is averaged and is carried out record.Write down each sperm viability.The gained data are following:
17 ℃ of constant temperature preservations of table 1 boar semen vigor changes statistics
Can find out from table 1, use the dilution powder of this prescription to dilute, seminal fluid sperm viability after preserving 7 days still can reach the semen deposition vigor requirement of boar normal temperature seminal fluid national standard still more than 0.6.Showing that this prescription can effectively prolong the holding time of seminal fluid, is a kind of dilution powder that boar semen is preserved that is suitable for, can large scale application.
Claims (9)
1. a boar semen dilution powder is filled a prescription, and it is characterized in that comprising the composition of following weight portion:
Add 1000 gram redistilled waters
2. according to the said boar semen dilution powder of claim 1, it is characterized in that also comprising a certain amount of antibiotic composition.
3. according to claim 1 or 2 said boar semen dilution powders, it is characterized in that each composition is the pure level of analysis chemicals.
4. according to claim 1 or 2 said boar semen dilution powders, it is characterized in that pulverizing to crossing the powder of 40 mesh sieves through air-cooled cracker.
5. according to the said boar semen dilution powder of claim 1, it is characterized in that each composition weighing of requirement accurately, mix, temperature below 20 ℃, relative moisture accomplishes weighing, pulverizing, mixing, packaging process in the gnotobasis below 50%.
6. the method for using of claim 1 or 2 said boar semen dilution powders is characterized in that may further comprise the steps:
(1) this dilution powder is dissolved in the ratio that per 50 grams add 1000 gram redistilled waters, be used to dilute seminal fluid after 30 minutes.
(2) temperature that need are regulated dilution according to the temperature of seminal fluid before the dilution seminal fluid, the two temperature difference is no more than 1 ℃.
(3) during the seminal fluid after the process dilution is slowly packed clean seminal fluid bottle or semen bag into, seal, lucifuge at room temperature leaves standstill the insulating box of putting into 17 ℃ after 1 hour and preserves, and whenever slowly shakes seminal fluid at a distance from 8 hours, avoids the sperm deposition to cause injures and deaths.
7. the method for using of claim 1 or 2 said boar semen dilution powders is slowly poured dilution in the seminal fluid into along chamber wall when it is characterized in that diluting seminal fluid, can not conversely seminal fluid be poured in the dilution.
8. the storage procedures of claim 1 or 2 said boar semen dilution powders is characterized in that finished product need be kept at cool place, dry place, temperature below 20 ℃, relative moisture is below 50%.
9. according to the said boar semen dilution powder of claim 1, it is characterized in that and directly to dissolve with the low temperature redistilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100762016A CN102687715A (en) | 2011-03-23 | 2011-03-23 | Cold-water instant pig seminal fluid dilution powder formula and manufacturing process and application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100762016A CN102687715A (en) | 2011-03-23 | 2011-03-23 | Cold-water instant pig seminal fluid dilution powder formula and manufacturing process and application method |
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| CN102687715A true CN102687715A (en) | 2012-09-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2011100762016A Pending CN102687715A (en) | 2011-03-23 | 2011-03-23 | Cold-water instant pig seminal fluid dilution powder formula and manufacturing process and application method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103518703A (en) * | 2013-10-14 | 2014-01-22 | 武鸣县水产畜牧兽医技术推广站 | Boar semen diluting agent |
| CN104068012A (en) * | 2014-06-17 | 2014-10-01 | 宜川欣源生态养殖专业合作社 | Formula of cold water instant type pig seminal fluid diluting powder as well as production process and application method |
| CN106417259A (en) * | 2016-10-26 | 2017-02-22 | 湖北省农业科学院畜牧兽医研究所 | Boar seminal fluid long-acting diluting powder and application thereof |
| CN114097769A (en) * | 2021-12-08 | 2022-03-01 | 山东道和生物科技有限公司 | Pig semen dilution powder and processing technology thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020182580A1 (en) * | 2000-09-11 | 2002-12-05 | Jean-Gerard Saint-Ramon | Extender for preserving porcine sperm cells |
| CN101062058A (en) * | 2007-06-05 | 2007-10-31 | 南京农业大学 | Tubule high-density type method for freezing pig jism and the products thereof |
| CN101543208A (en) * | 2009-03-31 | 2009-09-30 | 华南农业大学 | Powder for pig sperm dilution and room temperature preservation and preparation method and application thereof |
| CN101731199A (en) * | 2009-12-28 | 2010-06-16 | 杭州东源生物科技有限公司 | Preserving liquid for swine sperm |
| CN101803595A (en) * | 2010-03-26 | 2010-08-18 | 四川省畜牧科学研究院 | Diluent for storing the sperm of pig under normal temperature and preparation and dilution method thereof |
-
2011
- 2011-03-23 CN CN2011100762016A patent/CN102687715A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020182580A1 (en) * | 2000-09-11 | 2002-12-05 | Jean-Gerard Saint-Ramon | Extender for preserving porcine sperm cells |
| CN101062058A (en) * | 2007-06-05 | 2007-10-31 | 南京农业大学 | Tubule high-density type method for freezing pig jism and the products thereof |
| CN101543208A (en) * | 2009-03-31 | 2009-09-30 | 华南农业大学 | Powder for pig sperm dilution and room temperature preservation and preparation method and application thereof |
| CN101731199A (en) * | 2009-12-28 | 2010-06-16 | 杭州东源生物科技有限公司 | Preserving liquid for swine sperm |
| CN101803595A (en) * | 2010-03-26 | 2010-08-18 | 四川省畜牧科学研究院 | Diluent for storing the sperm of pig under normal temperature and preparation and dilution method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103518703A (en) * | 2013-10-14 | 2014-01-22 | 武鸣县水产畜牧兽医技术推广站 | Boar semen diluting agent |
| CN104068012A (en) * | 2014-06-17 | 2014-10-01 | 宜川欣源生态养殖专业合作社 | Formula of cold water instant type pig seminal fluid diluting powder as well as production process and application method |
| CN106417259A (en) * | 2016-10-26 | 2017-02-22 | 湖北省农业科学院畜牧兽医研究所 | Boar seminal fluid long-acting diluting powder and application thereof |
| CN114097769A (en) * | 2021-12-08 | 2022-03-01 | 山东道和生物科技有限公司 | Pig semen dilution powder and processing technology thereof |
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Application publication date: 20120926 |