CN104721184A - Preparation capable of improving cow conception rate as well as preparation method and application of preparation - Google Patents
Preparation capable of improving cow conception rate as well as preparation method and application of preparation Download PDFInfo
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- CN104721184A CN104721184A CN201510085043.9A CN201510085043A CN104721184A CN 104721184 A CN104721184 A CN 104721184A CN 201510085043 A CN201510085043 A CN 201510085043A CN 104721184 A CN104721184 A CN 104721184A
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Abstract
The invention relates to the field of animal husbandry and veterinary medicines, and particularly provides a preparation capable of improving the cow conception rate, as well as a preparation method and an application of the preparation. An effective component of the preparation comprises melatonin with the concentration of 10<-3> to 10<-1>mol/L and vitamin E (Ve) with the concentration of 10<-6> to 10<-4> mol/L. The animal experiment proves that when in-vitro injection of the preparation is carried out on breeding at the same time, the damage of reactive oxygen species (ROS) to quality of ovum can be reduced, and egg-sperm binding is promoted to achieve fertilization. According to the preparation, the cow conception rate can be effectively improved, and the reproduction efficiency of cows is improved.
Description
Technical field
The present invention relates to animal and veterinary field, specifically, relate to and a kind ofly improve preparation of milch cow conception rate and preparation method thereof and application.
Background technology
Along with improving constantly of people's living standard, annual milk consumption figure increases day by day, but the quantity of the nearly 2 years numerous cows of domestic energy is in rapid decline, the reproductive efficiency how improving milch cow is related to the reproductive speed of milch cow and the vital interests of raiser, is also an important indicator of scale field production management.The medicine of raising cow reproduction efficiency conventional is at present mainly gonadotrophins medicine, mainly promote cow ovaries function, as promoted the secretory function of follicular development, ovulation and corpus luteum, overcome the breeding difficulty caused thus, but the quality of mature egg can be had influence on, the requirement of satisfied raising cow reproduction efficiency that can not be not bad.
Melatonin (MT) is a kind of hormone primarily of pinus secretion, and chemical constitution is N-acetyl-5-hydroxytryptamine, in the Growth and reproduction of animal, have important adjusting function.In animal reproduction process, metabolism is very vigorous, can produce a large amount of active oxygens.Active oxygen can cause DNA Damage, causes apoptosis of granulosa cell, as long as there is the apoptosis of granulosa cell of 10% will cause follicular atresia (Manabe N etc., 2004) in 1 follicle.On the one hand, melatonin is combined the growth and ovulation of participating in follicle directly by antioxidant membrane receptor or nuclear receptor on ovary, maintains the stable state of steroid hormones; By its antioxidation Scavenger of ROS, suppress follicular cell apoptosis, guarantee carrying out smoothly of follicular development and ovulation process.On the other hand, research shows that melatonin can promote the maturation in vitro of oocyte and the ectogenesis of embryo.The test group blastocyst rate 68.85% (42/61) adding MT in the ectogenesis liquid of cattle embryo is significantly higher than matched group 50.79% (32/63) (Papis etc., 2007).More than study explanation: melatonin can improve cow reproduction efficiency by its antioxidation.
Cow reproduction is one of key factor affecting milk cow production performance performance.Research finds that superoxides is the important factor in follicle maturity and ovulation process, but superoxides can the quality of direct reduction mature egg.Utilize the method for subcutaneous injection composite antioxidant, contribute to the formation of polyphenoils peak value in milch cow maturation of ovum process, promote that polyphenoils is laid in follicle, after follicle maturity is broken, eliminate the impact of superoxides on ovum quality.The method is easy, easy to operate.
Summary of the invention
In order to solve problems of the prior art, the object of this invention is to provide and a kind ofly improve preparation of milch cow conception rate and preparation method thereof and application.
In order to realize the object of the invention, technical scheme of the present invention is as follows:
Improve a preparation for milch cow conception rate, described preparation is injection, and its effective ingredient is melatonin and vitamin E.
Further, the concentration of described melatonin is 10
-3~ 10
-1mol/L.
As preferably, the concentration of described melatonin is 10
-2mol/L.
Further, the concentration of described vitamin E is 10
-6~ 10
-4mol/L.
As preferably, the concentration of described vitamin E is 10
-5mol/L.
Further, the adjuvant of described preparation is 75% alcoholic solution or dehydrated alcohol.
Invention further provides the preparation method of described preparation, described method is: vitamin E is dissolved in 75% alcoholic solution or dehydrated alcohol, then adds melatonin, and whole operating process (lucifuge) need be carried out in darkroom.
Present invention also offers described preparation and improve the application in cow reproduction efficiency.
Further, when milch cow breeds by preparation described in subcutaneous injection, improve milch cow conception rate.
As preferably, injected dose is 2mL.
Beneficial effect of the present invention is:
The preparation of raising milch cow conception rate provided by the invention, melatonin and vitamin E are dissolved in certain proportion, that breeds milch cow passes through subcutaneous injection, can improve the conception rate of milch cow, and provides useful reference for carrying out the research improving cow reproduction efficiency further.
The present invention is found by zoopery, is 10 containing concentration
-2the preparation of mol/L melatonin can make the non-return rate of milch cow improve 9-12 percentage point, reaches 20-50%.Absolutely prove that hormone invention formulation simultaneously of breeding can reduce reactive oxygen free radical (Reactiveoxygen species, the ROS) damage to ovum quality, promoted that sperm ovum binding realizes fertilization.
In addition, the present invention realizes finding by animal, vitamin E makes there is obvious facilitation in milch cow non-return rate to the preparation containing melatonin in raising, use the preparation made according to claim 3 and 5 can improve milch cow non-return rate (44.09 ± 0.76%), be significantly higher than not containing vitamin E (37.35 ± 0.88%) or only containing the preparation of vitamin E (37.29 ± 0.49%).
Accompanying drawing explanation
Fig. 1 is the Changing Pattern of MT in holstein cow body in 24 hours in experimental example 1 of the present invention.
The change of antioxidant in body when Fig. 2 is milch cow puerperal and breeding in experimental example 1 of the present invention.
Detailed description of the invention
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
The reagent used in following examples and material are commercial goods.
Melatonin requires that purity reaches 99.9%.
Embodiment 1 improves preparation of milch cow conception rate and preparation method thereof
Raw material: melatonin, vitamin E;
Preparation method: precise 0.4307mg vitamin E, 232.27mg melatonin, first by 0.4307mg vitamin E with 75% dissolve with ethanol solution in 100mL volumetric flask, stir, and then add 232.27mg melatonin, stir, be settled to 100mL, be finally distributed into the injected dose of 2mL, all operations all completes in darkroom.
Embodiment 2
With embodiment 1, difference is, the concentration of described melatonin is 10
-1mol/L, the concentration of described vitamin E is 10
-4mol/L, and described vitamin E anhydrous alcohol solution.
Embodiment 3
With embodiment 1, difference is, the concentration of described melatonin is 10
-3mol/L, the concentration of described vitamin E is 10
-6mol/L.
Experimental example 1
1, the Changing Pattern of MT in holstein cow body in 24 hours
Multiparity holstein cow 7 (1-2 tire), normally searches for food, drinks water and milks.From morning 8, every 2 hr iv take a blood sample 10 milliliters, and centrifuging and taking serum is for subsequent use, until morning 8 next day point.Detect MT concentration in serum with liquid chromatography, measurement result is shown in Fig. 1.
As shown in Figure 1, in multiparity holstein cow 24 hours there is the change of obvious daily rhythmicity in melatonin secretion, and night, hormone-content was high, and daytime, content was low.
2, the change of MT in body when milch cow puerperal and breeding
Select multiparity holstein cow 22, from puerperal, adopt venous blood collection 10 milliliters in the morning 8 of every 4 days, and centrifuging and taking serum is for subsequent use, until this milch cow has bred.Detect MT concentration in serum with liquid chromatography, when successful holstein cow of breeding first is from puerperal to breeding, blood, MT concentration determination the results are shown in Figure 2.
As shown in Figure 2, in multiparity holstein cow serum in puerperal, melatonin concentration raises in time, and in cow serum during rutting, melatonin concentration reaches the highest.
3, cervical region subcutaneous injection while breeding
In 8-9 month, preparation 2mL described in subcutaneous injection embodiment 1 when breeding first puerperal to multiparity holstein cow (1-3 tire), takes the mode of random injection (every the mode of an injection, first injection, do not inject for second, the 3rd injection, by that analogy).
4, milch cow conception rate detects
Breed after 50-60 days, the method of examination per rectum is adopted to carry out pregnancy check to experiment holstein cow, statistics pregnancy rate, find to inject preparation of the present invention when milking cow is bred simultaneously, can significantly improve milch cow conception rate more than 20%, the highest increasing amount is close to 50% (see table 1, table 2).Wherein when summer, normal conception rate was lower slightly, the effect more outstanding (see table 1) of the raising milch cow conception rate of preparation of the present invention.
The conception rate situation of invention formulation is injected in the multiparity holstein cow breeding of table 1 scale cattle farm simultaneously
The conception rate situation of invention formulation is injected in the multiparity holstein cow breeding of table 2 Animal Raising Zone simultaneously
These results suggest that, there is circadian changes in multiparity holstein cow melatonin secretion, to oestrus serum, melatonin content significance raises, and now breeds and injects composite antioxidant, melatonin content in further raising serum, can improve the conception rate of milch cow significantly.
Comparative example 1
1, cervical region subcutaneous injection vitamin C (Vitamin C, Vc) and Vitamin E preparation (2mL) while breeding
In 4-5 month, subcutaneous injection vitamin C and Vitamin E preparation 2mL when breeding first puerperal to multiparity holstein cow (1-3 tire), ascorbic concentration is 10
-2mol/L, the concentration of vitamin E is 10
-5mol/L, take random injection mode (every one injection mode, first injection, does not inject for second, the 3rd injection, by that analogy).
2, milch cow conception rate detects
Breed after 50-60 days, the method of examination per rectum is adopted to carry out pregnancy check to experiment holstein cow, statistics pregnancy rate, find to inject vitamin C and Vitamin E preparation when milking cow is bred simultaneously, milch cow conception rate about 5% can be improved, with matched group without significant difference (P>0.05), but significantly lower than the action effect (see table 3) of preparation of the present invention.
Table 3 invention formulation and vitamin C and Vitamin E preparation improve milch cow conception rate situation and compare
Comparative example 2
1, the preparation (2mL) of cervical region subcutaneous injection different ratio while breeding
11-12 month in 2013 and 1-5 month in 2014, when breeding first puerperal to multiparity holstein cow (1-3 tire), the preparation 2mL of subcutaneous injection different ratio of the present invention, mainly contains MT (10
-2mol/L)+Ve (0mol/L); MT (10
-1mol/L)+Ve (10
-4mol/L); MT (10
-2mol/L)+Ve (10
-5mol/L); MT (10
-3mol/L)+Ve (10
-6mol/L); MT (0mol/L)+Ve (10
-5mol/L) 5 kinds.Take the mode of random injection (mode of injecting in turn, first injection MT (10
-2mol/L)+Ve (0mol/L), second injection MT (10
-1mol/L)+Ve (10
-4mol/L), the 3rd injection MT (10
-2mol/L)+Ve (10
-5mol/L), four-head injection MT (10
-3mol/L)+Ve (10
-6mol/L), the 5th injection MT (0mol/L)+Ve (10
-5mol/L), by that analogy.
2, milch cow conception rate detects
Breed after 50-60 days, adopt the method for examination per rectum to carry out pregnancy check to experiment holstein cow, statistics pregnancy rate, find that the preparation of injection injection different ratio simultaneously when milking cow is bred all can improve milch cow conception rate about, but difference on effect is comparatively large, MT (10
-1mol/L)+Ve (10
-4mol/L), MT (10
-2mol/L)+Ve (10
-5and MT (10 mol/L)
-3mol/L)+Ve (10
-6mol/L) three kinds of proportionings improve the Be very effective (P<0.05) of milch cow conception rate higher than contrast, MT (10
-2mol/L)+Ve (0mol/L) and MT (0mol/L)+Ve (10
-5mol/L) group.Matched group, MT (10
-2mol/L)+Ve (0mol/L) group and MT (0mol/L)+Ve (10
-5mol/L) between group without significant difference (P>0.05) (see table 4).
The preparation of table 4 different ratio of the present invention improves milch cow conception rate situation and compares
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. improve a preparation for milch cow conception rate, it is characterized in that, described preparation is injection, and its effective ingredient is melatonin and vitamin E.
2. preparation according to claim 1, is characterized in that, the concentration of described melatonin is 10
-3~ 10
-1mol/L.
3. preparation according to claim 2, is characterized in that, the concentration of described melatonin is 10
-2mol/L.
4. the preparation according to any one of claim 1-3, is characterized in that, the concentration of described vitamin E is 10
-6~ 10
-4mol/L.
5. preparation according to claim 4, is characterized in that, the concentration of described vitamin E is 10
-5mol/L.
6. preparation according to claim 5, is characterized in that, the adjuvant of described preparation is 75% alcoholic solution or dehydrated alcohol.
7. the preparation method of preparation described in any one of claim 1-6, is characterized in that, described method is: vitamin E is dissolved in 75% alcoholic solution or dehydrated alcohol, then adds melatonin, and operating process lucifuge is carried out.
8. preparation described in any one of claim 1-6 is improving the application in cow reproduction efficiency.
9. application according to claim 8, is characterized in that, when milch cow breeds by preparation described in subcutaneous injection, improves milch cow conception rate.
10. application according to claim 9, is characterized in that, injected dose is 2mL.
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Cited By (8)
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| CN106474133A (en) * | 2016-09-21 | 2017-03-08 | 北京市农林科学院 | A kind of preparation improving milch cow conception rate and its application |
| CN106511386A (en) * | 2016-10-19 | 2017-03-22 | 北京市农林科学院 | Bovine frozen semen preparation and preparation method thereof |
| CN109042515A (en) * | 2018-08-16 | 2018-12-21 | 阜阳师范学院 | Improve the attached method for planting quantity and nest litter size of body early embryo in goat body |
| CN110637929A (en) * | 2019-09-27 | 2020-01-03 | 中国农业大学 | Method for increasing litter size of sows by feeding melatonin before and after oestrus |
| CN110720410A (en) * | 2019-10-14 | 2020-01-24 | 浙江海洋大学 | Method for improving fertilization rate of zebra fish |
| CN111183955A (en) * | 2020-01-03 | 2020-05-22 | 中国农业大学 | Method for improving reproductive performance of mammals after pregnancy by oral administration of melatonin |
| CN116171932A (en) * | 2023-02-24 | 2023-05-30 | 北京首农畜牧发展有限公司 | Integrated method for improving melatonin content in milk |
| CN116270618A (en) * | 2023-05-05 | 2023-06-23 | 中国人民解放军海军军医大学第一附属医院 | Melatonin and vitamin E pharmaceutical composition and application thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106474133A (en) * | 2016-09-21 | 2017-03-08 | 北京市农林科学院 | A kind of preparation improving milch cow conception rate and its application |
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| CN106511386A (en) * | 2016-10-19 | 2017-03-22 | 北京市农林科学院 | Bovine frozen semen preparation and preparation method thereof |
| CN106511386B (en) * | 2016-10-19 | 2019-12-06 | 北京市农林科学院 | Frozen bovine semen preparation and preparation method thereof |
| CN109042515A (en) * | 2018-08-16 | 2018-12-21 | 阜阳师范学院 | Improve the attached method for planting quantity and nest litter size of body early embryo in goat body |
| CN110637929A (en) * | 2019-09-27 | 2020-01-03 | 中国农业大学 | Method for increasing litter size of sows by feeding melatonin before and after oestrus |
| CN110720410A (en) * | 2019-10-14 | 2020-01-24 | 浙江海洋大学 | Method for improving fertilization rate of zebra fish |
| CN110720410B (en) * | 2019-10-14 | 2021-08-20 | 浙江海洋大学 | Method for improving fertilization rate of zebra fish |
| CN111183955A (en) * | 2020-01-03 | 2020-05-22 | 中国农业大学 | Method for improving reproductive performance of mammals after pregnancy by oral administration of melatonin |
| CN116171932A (en) * | 2023-02-24 | 2023-05-30 | 北京首农畜牧发展有限公司 | Integrated method for improving melatonin content in milk |
| CN116270618A (en) * | 2023-05-05 | 2023-06-23 | 中国人民解放军海军军医大学第一附属医院 | Melatonin and vitamin E pharmaceutical composition and application thereof |
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