CN101720695A - Method and isolated plant for hatching abalone fertilized ova by electric field excitation - Google Patents
Method and isolated plant for hatching abalone fertilized ova by electric field excitation Download PDFInfo
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- CN101720695A CN101720695A CN200910220731A CN200910220731A CN101720695A CN 101720695 A CN101720695 A CN 101720695A CN 200910220731 A CN200910220731 A CN 200910220731A CN 200910220731 A CN200910220731 A CN 200910220731A CN 101720695 A CN101720695 A CN 101720695A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses method and isolated plant for hatching abalone fertilized ova by electric field excitation, which can increase the hatchability and the survival rate of abalone artificial breeding and reduce the death rate of young stripped abalones. The method comprises the following steps of: selecting and then placing abalone fertilized ova after being fertilized for 20-50min in the environment of a uniform electric field the field intensity of which is 8-14kV/cm, and then placing the abalone fertilized ova excited by the electric field for incubation. The isolated plant is provided with a closed outer housing (1); the upper and lower ends in the closed outer housing (1) are respectively fixed with an upper polar plate (2) and a lower polar plate (3) which are connected with a high-voltage power supply and can generate 5-15kV/cm uniform electric field; the lower polar plate (3) is provided with an insulating mat (4); and the insulating mat (4) is provided with an insulating sample room (5).
Description
Technical field:
The present invention relates to the technical field of aquaculture, especially a kind of incubation rate and emergence rate that improves artificial abalone fry culture, the method and the isolated plant of the hatching abalone fertilized ova by electric field excitation of the young Bao lethality after minimizing is peeled off.
Background technology:
Bao (being commonly called as abalone) is a kind of shellfish with very high economic worth, is listed in first of the marine products eight delicacies.Along with improving constantly of living standards of the people, increase day by day for the demand of seafood especially precious marine product, still can not satisfy the demand because of the supply of precious marine product in the international market, so price rises steadily, the speed of growth has surpassed the several times of normal food.In recent years, though artificial breeding technique of Bao and aquaculture technology the earth to the utmost have promoted the development of foster Bao Ye, conventional artificial raise seedling method also exists incubation rate and emergence rate is low, the young Bao lethality height after peeling off or the like problem.
Summary of the invention:
The present invention is in order to solve the above-mentioned technical problem of existing in prior technology, a kind of incubation rate and emergence rate that improves artificial abalone fry culture to be provided, reducing the method and the isolated plant of the hatching abalone fertilized ova by electric field excitation of the young Bao lethality after peeling off.
Technical solution of the present invention is: a kind of method of hatching abalone fertilized ova by electric field excitation, it is uniform electric field environment 10～30min of 8～14kv/cm that the abalone fertilized ova that it is characterized in that choosing after fertilization 20～50min places field intensity, and then will be put in the incubation tank by the abalone fertilized ova of electric field excitation and hatch.
A kind of hatching abalone fertilized ova by electric field excitation method device, it is characterized in that: be provided with closed cover, upper and lower side is fixed with top crown, the bottom crown that joins with high voltage source and can produce 8～14kv/cm uniform electric field respectively in closed cover, on bottom crown, be equipped with felt pad, be equipped with the sample room of insulation on the felt pad.
Described top crown, bottom crown and felt pad are round, and the sample room of described insulation is a cylindrical shape, and the diameter of described top crown, bottom crown is the twice of sample room at least.
Be fixed with lower carriage in described closed cover, described bottom crown is fixed on the lower carriage; Described top crown joins with the vertical connecting rod that passes the closed cover top cover, and vertically connecting rod joins by transverse link and arrangement for adjusting height.
The present invention is based on the basic principle of electromagnetic environment biological effect, rationally utilize external environment electric field excitation abalone development of fertilized ova, can improve the incubation rate and the emergence rate of Bao artificial breeding, reduce the young Bao lethality after peeling off, the survival rate of young Bao and the adaptive capacity of young Bao Shengchang later stage environmental change have been improved, the present invention simultaneously can not cause biotic habitat problems such as abalone gene mutation, non-secondary pollution, for abalone culture already provides good seedling.
Description of drawings:
Fig. 1 is the structural representation of embodiment of the invention equipment therefor.
Fig. 2 is the curve map of the embodiment of the invention to the influence of hatching abalone fertilized ova rate.
Embodiment:
The method of the embodiment of the invention be the abalone fertilized ova of choosing after fertilization 20～50min to place field intensity be uniform electric field environment 10～30min of 8～14kv/cm, and then will be put in the incubation tank by the abalone fertilized ova of electric field excitation and hatch.
Embodiment of the invention equipment therefor is to be provided with the closed cover made from the insulation material 1, upper and lower side is fixed with top crown 2, the bottom crown 3 that joins with high voltage source and can produce 8～14kv/cm uniform electric field respectively in closed cover 1, on bottom crown 3, be equipped with felt pad 4, be equipped with the sample room 5 of insulation on the felt pad 4, regulate the high voltage source output voltage, or the distance between adjustment top crown 2 and the bottom crown 3, make between top crown 2, the bottom crown 3 and produce 8～14kv/cm uniform electric field.
Described top crown 2, bottom crown 3 and felt pad 4 are round, and the sample room 5 of described insulation is a cylindrical shape, and in order to obtain uniform electric field, the diameter of described top crown 2, bottom crown 3 is the twice of sample room 5 at least.
Be fixed with lower carriage 6 in described closed cover 1, described bottom crown 3 is fixed on the lower carriage 6; Described top crown 2 joins with the vertical connecting rod 7 that passes closed cover 1 top cover, and vertically connecting rod 7 joins by transverse link 8 and arrangement for adjusting height 9.Arrangement for adjusting height 9 can be existing rod-type rotary extension self-locking device, also can be that a vertical rod is set, the sleeve pipe that joins with transverse link 8 is arranged in vertical rod, the a plurality of holes that on the vertical direction of vertical rod and sleeve pipe, are processed with respectively, by moving up and down sleeve pipe and fixing with pin, finish the height of sleeve pipe and regulate, promptly finish the distance adjustment between top crown 2 and the bottom crown 3.
Experimental example:
The embodiment of the invention acts on the influence of 40 minutes abalone fertilized ova of after fertilization to incubation rate
1. experiment material
The haliotis discus hannai Ino that female, male close Bao Junwei the northern area of China is cultured by manually storing up close Bao, can make the close Bao long period keep maturity state, and by induced spawning and test-tube method, obtaining can be for the fertilized egg of experiment.
2. instrument and equipment
The device of the embodiment of the invention is used in experiment; As requested, produce the electric field of 0～15kv/cm in control electric power output voltage or the adjusting top crown height effector on the scene.
When observation fertilized egg and hatching situation thereof quantitative, adopt blood counting chamber and Japanese Olympus light microscope
3. experimental technique
According to set electric-field intensity condition number N, the fertilized egg of abalone is divided into the N+1 part that approximately equates.Wherein organize in contrast for 1 part, other N part fertilized egg places the sample room of field-effect device to carry out electric field excitation respectively.Respectively with intensity be 0,2,4,6,8,10,12, the electric field excitation 0,5,10,15,20 of 14kv/cm, 30min; Fertilized egg after the excitation is hatched under identical environmental condition with unperturbed fertilized egg, grows trochophora when it and carries out quantitative observation period.
With abalone fertilized egg hatching rate is observation index, promptly use blood counting chamber, ghost number in microscopically observation unit water body, lopsided number and the number of normally surviving, wherein three's sum is total ovum amount, the ratio that sum and total ovum amount are counted in deformity number and normal survival is an incubation rate, and the ratio of normally survive number and total ovum amount is an emergence rate.In quantitative process, to the sample of each treatment conditions, take out three samples simultaneously, each sample numeration 3～6 times, its mean value is as final result.
4. experimental result
Experimental result shows that the incubation rate of abalone fertilized ova increases along with the increase of electric-field intensity (abbreviation field intensity) and actuation duration.
As seen from Figure 1, for the actuation duration is four groups of experiments of 10min, 15min, 20min and 30min, field intensity is very close to the empirical curve of abalone hatchability of fertile eggs influence, and promptly field intensity is in the 0-4kv/cm interval, and the incubation rate of control group and experimental group is more or less the same; Field intensity incubation rate in 4～7kv/cm interval almost is linear increasing, and amplitude of variation is very big; Field intensity is during greater than 7kv/cm, and the incubation rate empirical curve is close to and is parallel to axis of abscissas, and incubation rate is tending towards a metastable value.For the actuation duration is that two groups of empirical curves of 3min and 5min demonstrate because the actuation duration is shorter, causes incubation rate always to be lower than four groups of long experiments of actuation duration; And to be 30 minutes empirical curve demonstrate the actuation duration, and after electric-field intensity was greater than 8kv/cm, incubation rate began to reduce.When actuation duration one timing, abalone fertilized egg hatching rate increases with the increase of electric-field intensity, after field intensity is increased to certain numerical value, abalone fertilized egg hatching rate reduces lentamente along with the increase of electric-field intensity again, and this shows that the field intensity factor is the deciding factor to the influence of abalone hatchability of fertile eggs.Specifically, when electric-field intensity during less than 4kv/cm, field intensity is little to the influence of abalone fertilized egg hatching rate; Powerful then and there when 4kv/cm, field intensity to the influence of abalone fertilized egg hatching rate obviously.When field intensity surpassed 10kv/cm, incubation rate had a declining tendency.When field intensity was 10kv/cm, the incubation rate of abalone fertilized ova approached peak value.
In a word, the hatching abalone fertilized ova by electric field excitation effect is obvious, particularly when field intensity be 8～14kv/cm, actuation duration during greater than 10min, the incubation rate of abalone fertilized ova is more than 85%, exceeds about 10 percentage points than hatching naturally.
Other has experimental result to show, abalone fertilized ova by electric field excitation not only incubation rate improves, and emergence rate also has a more substantial increase, and to the late growing stage of young Bao and to adaptive capacity to environment facilitation is arranged all also simultaneously.
Claims (4)
1. the method for a hatching abalone fertilized ova by electric field excitation, it is uniform electric field environment 10～30min of 8～14kv/cm that the abalone fertilized ova that it is characterized in that choosing after fertilization 20～50min places field intensity, and then will be put in the incubation tank by the abalone fertilized ova of electric field excitation and hatch.
2. hatching abalone fertilized ova by electric field excitation method device, it is characterized in that: be provided with closed cover (1), upper and lower side is fixed with top crown (2), the bottom crown (3) that joins with high voltage source and can produce 8～14kv/cm uniform electric field respectively in closed cover (1), on bottom crown (3), be equipped with felt pad (4), be equipped with the sample room (5) of insulation on the felt pad (4).
3. hatching abalone fertilized ova by electric field excitation method device according to claim 2, it is characterized in that: described top crown (2), bottom crown (3) and felt pad (4) are round, the sample room of described insulation (5) is a cylindrical shape, and the diameter of described top crown (2), bottom crown (3) is the twice of sample room (5) at least.
4. according to claim 2 or 3 described hatching abalone fertilized ova by electric field excitation method devices, it is characterized in that: be fixed with lower carriage (6) in described closed cover (1), described bottom crown (3) is fixed on the lower carriage (6); Described top crown (2) joins with the vertical connecting rod (7) that passes closed cover (1) top cover, and vertically connecting rod (7) joins by transverse link (8) and arrangement for adjusting height (9).
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910220731A CN101720695A (en) | 2009-12-14 | 2009-12-14 | Method and isolated plant for hatching abalone fertilized ova by electric field excitation |
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| CN200910220731A CN101720695A (en) | 2009-12-14 | 2009-12-14 | Method and isolated plant for hatching abalone fertilized ova by electric field excitation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961000A (en) * | 2010-10-29 | 2011-02-02 | 中国农业科学院植物保护研究所 | Method for breeding large amount of Stratiolaelaps scimitus and Hypoaspis aculeifer (Canestrini) manually |
| CN106771723A (en) * | 2016-11-30 | 2017-05-31 | 广东电网有限责任公司佛山供电局 | A kind of time adjustable power current acts on the experimental provision of fish body |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961000A (en) * | 2010-10-29 | 2011-02-02 | 中国农业科学院植物保护研究所 | Method for breeding large amount of Stratiolaelaps scimitus and Hypoaspis aculeifer (Canestrini) manually |
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| CN106771723A (en) * | 2016-11-30 | 2017-05-31 | 广东电网有限责任公司佛山供电局 | A kind of time adjustable power current acts on the experimental provision of fish body |
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Application publication date: 20100609 |