CN101716295A - Chinese medicinal composition containing Chinese yams, and application thereof - Google Patents

Abstract

The invention provides a Chinese medicinal composition containing Chinese yams. The composition consists of the following components in mass ratio: 5 to 25 parts of Chinese yams, 5 to 25 parts of coix seeds and 1 to 15 parts of tangerine peel. The Chinese medicinal composition containing Chinese yams has the main advantages of: (1) reasonable formulation, function of invigorating spleen and regulating qi and capability of effectively regulating astrointestinal motor function; (2) capability of improving stress ability of organisms; (3) capability of regulating hormone level and immune function of stomachs-intestines with functional dyspepsia; and (4) capability of improving the ability of the organisms to clear oxygen free radicals.

Description

A kind of Chinese medicine composition and application thereof that contains Rhizoma Dioscoreae

(1) technical field

The present invention relates to a kind of Chinese medicine composition that contains Rhizoma Dioscoreae, and the application in preparation control functional dyspepsia medicine.

(2) background technology

Functional dyspepsia (Functional Dyspepsia, FD) be meant have glutted after the meal discomfort, early full, belch, feel sick, symptoms such as vomiting, upper abdominal pain or upper abdomen burn feeling, get rid of one group of clinical syndrome of the organic disease that causes these symptoms on inspection, Rome III standard is divided into uncomfortable after the meal syndrome and Upper abdominal pain syndrome with it.Its morbidity is very general, and everyone nearly all had dyspeptic symptom in life.Western countries' epidemiological study shows has 15%～20% people to live through dyspepsia every year, and domestic statistics accounts for 18.9% of normal population, accounts for about 40% of Digestive System Department patient number, accounts for gastrointestinal special outpatient clinic patient's 1/3.And along with rhythm of life is accelerated day by day, its morbidity is more and more extensive.Though FD does not jeopardize people's life, have a strong impact on people's study and work and quality of life, taken quite high medical expense.Although the primary disease prognosis is good relatively, secular dyspepsia can influence patient's body constitution, thereby makes immunity degradation, is in a kind of sub-health state, is easy to infect other diseases, thereby this disease becomes main health care problem in the modern society gradually.In addition, because the pathogenesis of FD is not illustrated as yet fully, so its treatment aspect does not still have specific medicament, doctor trained in Western medicine is confined to symptomatic treatment more, and drug resistance and side effect are cut and easily produced in uncertain therapeutic efficacy; Though the Chinese medicine decoction good effect mostly is the individualized treatment scheme, be difficult for promoting.Thereby how effectively to prevent and treat the focus that FD has become at present clinical and scientific research.In light of this situation, clinically press for a kind of good efficacy that had both possessed Chinese medicine, be easy to carry out the group feature treatment again, and the Chinese patent medicine of taking convenience.

(3) summary of the invention

The present invention seeks in order to provide a kind of determined curative effect, taking convenience, medicine source abundant, cheap, the Chinese medicine composition that contains Rhizoma Dioscoreae that can effectively regulate the gastrointestinal motility function of no obvious toxic-side effects, and the application of this Chinese drug-treated group conjunction thing in preparation control functional dyspepsia medicine.

The technical solution used in the present invention is:

A kind of Chinese medicine composition that contains Rhizoma Dioscoreae, form by the component that the quality proportioning is following:

5～25 parts of Rhizoma Dioscoreaes

5～25 parts of Semen Coiciss

1～15 part of Pericarpium Citri Reticulatae.

Preferably, described composition quality proportioning is as follows:

10 parts of Rhizoma Dioscoreaes

10 parts of Semen Coiciss

5 parts of Pericarpium Citri Reticulataes.

Rhizoma Dioscoreae sweet in the mouth wherein, property is flat, returns spleen, lung, kidney channel, has supplementing QI and nourishing YIN, the effect of spleen reinforcing stomach reinforcing.Modern pharmacological research shows that Rhizoma Dioscoreae mainly contains compositions such as diosgenin, allantoin, polysaccharide, can regulate body's immunity and gastrointestinal motility function.The Semen Coicis slightly sweet flavor is returned spleen, stomach, lung meridian, has promoting diuresis to eliminate damp pathogen, invigorating spleen and reinforcing stomach, and the effect of clearing away heat and discharging pus eliminating impediment, cold nature and not injuring one's stomach, strengthening the spleen and do not grow greasyly is a good merchantable brand of mending dampness removing clearly.Semen Coicis contains Semen Coicis fat, crude protein, lipid, also contains glucosan and multiple volatile oil, has effects such as enhancing body humoral immunization, analgesia, analgesic, antiinflammatory.Pericarpium Citri Reticulatae bitter in the mouth, suffering, warm in nature, return lung, spleen channel, have regulating qi-flowing for strengthening spleen, the effect of drying dampness to eliminate phlegm, Pericarpium Citri Reticulatae mainly contains compositions such as volatile oil, flavonoid, organic amine and trace element, has the ptyalin of promotion enzymatic activity, promote mice gastric emptying and the propulsive effect of intestinal, its volatile oil has demulcent stimulation to digestive tract, is beneficial to the discharge of gastrointestinal pneumatosis.Three medicines share, clear tonifying the spleen and stomach, strong fortune Central Region, orderly and clearly mechanism of qi.This side's prescription thought is clear and definite, the compatibility refine, and power is specially imitated grand, has demonstrated fully " the spleen invigorating key is at activating the spleen " purport, suits the main pathogenesis of gastrointestinal motility dysfunction---and spleen fortune is lost strong, disorder of movement of QI.Its clinical practice all obtains good curative effect for many years on treatment functional digestive disease.

The effective ingredient of described Chinese medicine composition decoction and alcohol sedimentation technique routinely carries out, concrete, described method comprises: the Rhizoma Dioscoreae of getting formula ratio, Semen Coicis and Pericarpium Citri Reticulatae, the adding quality is a Rhizoma Dioscoreae, the water of Semen Coicis and 1.5～5 times of quality of Pericarpium Citri Reticulatae gross mass, reflux, extract, 1～3 time, each 0.5～2 hour, merge aqueous extract, filter, get filtrate decompression concentrate clear paste, it is 60～70% (v/v) that clear paste stirs ethanol to the ethanol final concentration that adds 80～95% (v/v), leaves standstill more than 24 hours, get supernatant, cross the leaching decompression filtrate recycling ethanol, concentrate, get Chinese medicine extract.

The invention still further relates to the application of described Chinese medicine composition in preparation control functional dyspepsia medicine.

Described medicine can be made into one of following dosage form: 1. capsule, 2. granule, 3. powder, 4. tablet.

Functional dyspepsia is a kind of gastrointestinal motility disorder disease.Its morbidity is closely related with physical stress ability drop, gut hormone [as motilin (MTL), vasoactive intestinal peptide (VIP), nitric oxide (NO) etc.] imbalance, oxygen radical removing systemic-function and immunologic function degression.The Chinese medicine composition that contains Rhizoma Dioscoreae of the present invention can not only improve the stress ability of body, and also the gastrointestinal motility to different conditions plays different regulating actions: under normal circumstances promptly, gastrointestinal motility is had certain inhibitory action; And, can improve the content of its MTL to the functional dyspepsia person, and the content of its VIP is obviously reduced, reduce serum NO content and NOS vigor, reduce MDA content, the increased SOD vigor can improve index and spleen index.Reach therapeutical effect by gut hormone, oxygen radical removing system and the many target spots of immunologic function, multipath to functional dyspepsia.

The beneficial effect that contains the Chinese medicine composition of Rhizoma Dioscoreae of the present invention is mainly reflected in: (1) composite formula provided by the invention is reasonable, has the effect that spleen invigorating is regulated the flow of vital energy, and can effectively regulate the gastrointestinal motility function; (2) can improve the physical stress ability; (3) energy dyspeptic gut hormone level of regulatory function and immunologic function; (4) can improve the ability that body is removed oxygen-derived free radicals.

(4) specific embodiment

The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:

Embodiment 1: contain the preparation of the Chinese medicine composition extract of Rhizoma Dioscoreae

1, prescription

Rhizoma Dioscoreae 100g

Semen Coicis 100g

Pericarpium Citri Reticulatae 50g

2, preparation method

Get above-mentioned prescription medical material, extracting in water 2 times adds 3 times of water gagings for the first time, soaked 0.5 hour, extracted 1.5 hours, add 1.5 times of water gagings for the second time, extracted 1 hour, collecting decoction filters, filtrate decompression is concentrated into the clear paste of 0.8g/ml, and qinghuo reagent stirs the ethanol that adds 95% (v/v), making the ethanol ultimate density is 65% (v/v), leaves standstill 24 hours, gets supernatant, filter, decompression filtrate recycling ethanol also is concentrated into every milliliter of crude drug content 12.5g.

Embodiment 2:

1, prescription

Rhizoma Dioscoreae 105g

Semen Coicis 105g

Pericarpium Citri Reticulatae 40g

2, preparation method

Get above-mentioned prescription medical material, extracting in water 2 times adds 3 times of water gagings for the first time, soaked 0.5 hour, extracted 1.5 hours, add 1.5 times of water gagings for the second time, extracted 1 hour, collecting decoction filters, filtrate decompression is concentrated into the clear paste of 0.9g/ml, and qinghuo reagent stirs the ethanol that adds 95% (v/v), making the ethanol ultimate density is 70% (v/v), leaves standstill 24 hours, gets supernatant, filter, decompression filtrate recycling ethanol also is concentrated into every milliliter of crude drug content 12.5g.

Embodiment 3:

1, prescription

Rhizoma Dioscoreae 100g

Semen Coicis 95g

Pericarpium Citri Reticulatae 55g

2, preparation method

Get above-mentioned prescription medical material, extracting in water 2 times adds 2 times of water gagings for the first time, soaked 1 hour, extracted 2 hours, add 1.5 times of water gagings for the second time, extracted 1 hour, collecting decoction filters, filtrate decompression is concentrated into the clear paste of 0.8g/ml, and qinghuo reagent stirs the ethanol that adds 90% (v/v), making the ethanol ultimate density is 60% (v/v), leaves standstill 24 hours, gets supernatant, filter, decompression filtrate recycling ethanol also is concentrated into every milliliter of crude drug content 12.5g.

Embodiment 4:

1, prescription

Rhizoma Dioscoreae 95g

Semen Coicis 105g

Pericarpium Citri Reticulatae 50g

2, preparation method

Get above-mentioned prescription medical material, extracting in water 2 times adds 3 times of water gagings for the first time, soaked 1 hour, extracted 2 hours, add 2 times of water gagings for the second time, extracted 1 hour, collecting decoction filters, filtrate decompression is concentrated into the clear paste of 0.8g/ml, and qinghuo reagent stirs the ethanol that adds 95% (v/v), making the ethanol ultimate density is 65% (v/v), leaves standstill 24 hours, gets supernatant, filter, decompression filtrate recycling ethanol also is concentrated into every milliliter of crude drug content 12.5g.

Embodiment 5: contain the research of the Chinese medicine composition extract of Rhizoma Dioscoreae to the bowel movement effect of exsomatizing

1, laboratory animal

Healthy Japan large ear rabbit, regular grade, male and female are regardless of, and body weight 2～2.5Kg is provided by Zhejiang University of Traditional Chinese Medicine Animal Experimental Study center.

2, experimental drug and reagent

Described combined extracts is according to embodiment 1 preparation; Tyrode's solution: be made into distilled water and contain NaCl 8.0g, KCl 0.2g, MgSO ₄7H ₂O 0.26g, NaH ₂PO ₄2H ₂O 0.065g, NaHCO ₃1.0g, CaCl ₂0.20g, the 1000ml solution of glucose 1.0g, standby; The used NaCl of preparation tyrode's solution, KCl, MgSO ₄7H ₂O, NaH ₂PO ₄2H ₂O, NaHCO ₃, CaCl ₂, glucose is analytical pure.

3, experiment equipment

Trace many diversions body organ level bath and necessary accessories: Zhejiang University of Traditional Chinese Medicine Animal Experimental Study center provides; MedLab bio signal acquisition processing system: Meiyi Science ﹠ Technology Co., Ltd., Nanjing; Adjustment of tonicity transducer: Nanjing Immunivest Corp..

4, experimentation

(1) experimental technique

Get healthy rabbits, fasting 24h before the experiment, freely drink water, cut the abdominal cavity after the stunning immediately open, find out stomach and duodenum intersection, take out one section duodenum intestinal segment, find apart from ileocecus 10cm place, get one section ileum, separate mesentery and its content is rinsed well with tyrode's solution, be cut into 2～3cm intestinal tube respectively, be stored in low temperature (4～6 ℃) and contain gaseous mixture (95%O ₂+ 5%CO ₂) in the saturated tyrode's solution, standby.

By the intestinal smooth muscle perfusion experimental technique that exsomatizes, with automatic oxygen supply sustainable supply gaseous mixture (95%O ₂+ 5%CO ₂), intestinal tube one end is fixed on the ventilation hook, the other end is connected and fixed on tonotransducer and causes computer interface.Water-bath keeps in the bath temperature (37 ± 0.5) ℃, feeds 60～80 bubble/min of gaseous mixture, specimen load 1g, and chart speed 4mm/min, range 20mv, balance 30～45min, every 15min renews bright tyrode's solution once between the specimen stable phase.Write down one section normal contraction curve, begin to add tested medicine.Medicine directly adds in the thermostatic bath with microsyringe.Each administration volume 0.1ml after every experiment finishes, washes specimen with tyrode's solution, and each 10min at interval totally 3 times, treats just to carry out next step experiment after the intestinal segment contraction is stablized.

(2) experiment content

Specimen is stablized 1h, after producing the constant self-disciplining of rule, the state smoothing of record contrast earlier flesh activity curve 5min, add the Chinese medicine composition extract that contains Rhizoma Dioscoreae of various dose then in bath, making its final concentration is (0.025g/ml, 0.050g/ml, 0.100g/ml, 0.200g/ml) (in contained crude drug amount), observe 3min after each dosing, amplitude, mean tension and frequency change that the record medicine is had an effect.

(3) statistical method

All (x ± S) expression carries out statistical analysis with SPSS software statistics bag to each group data to every data with mean ± standard deviation.Two groups of means are relatively with the t check, and mean is relatively used variance analysis between many groups, relatively checks with q in twos between mean.

5, experimental result

The result shows, this combined extracts is obvious inhibitory action to stripped duodenum of rabbit and ileum autonomic movement, show as amplitude and weaken (P＜0.01 or P＜0.05), tonicity decline (P＜0.01 or P＜0.05), frequency deceleration (P＜0.01 or P＜0.05), and strengthen with dosage, effect is strengthened.

(1) to the influence of the duodenum autonomic movement amplitude that exsomatizes, tension force, frequency

Table 1 shows, and compares before the administration, and the amplitude of each dosage group of combined extracts all obviously reduces (P＜0.01); Be tangible dose-effect relationship between each dosage group, compare with the 0.025g/ml group, 0.050g/ml, 0.100g/ml and 0.200g/ml group amplitude obviously reduce (P＜0.01); Compare with the 0.050g/ml group, 0.100g/ml and 0.200g/ml group amplitude reduce (P＜0.01).

With compare before the administration, the tension force of each dosage group of combined extracts all obviously reduces (P＜0.01 or P＜0.05); Be tangible dose-effect relationship between each dosage group, compare with the 0.025g/ml group, 0.100g/ml and 0.200g/ml group tension force obviously reduce (P＜0.01 or P＜0.05); Compare with the 0.050g/ml group, 0.200g/ml group tension force reduces (P＜0.01); Compare with the 0.100g/ml group, 0.200g/ml group tension force reduces (P＜0.05).

With compare before the administration, concentration is that the frequency of 0.200g/ml group reduces (P＜0.05); Compare with the 0.025g/ml group, 0.100g/ml and 0.200g/ml class frequency obviously reduce (P＜0.01 or P＜0.05); Compare with the 0.050g/ml group, 0.100g/ml and 0.200g/ml class frequency reduce (P＜0.01 or P＜0.05).

(2) to the influence of the ileum autonomic movement amplitude that exsomatizes, tension force, frequency

Table 2 shows, and compares before the administration, and the amplitude of each dosage group all obviously reduces (P＜0.01 or P＜0.05); Be tangible dose-effect relationship between each dosage group, compare with the 0.025g/ml group, 0.050g/ml, 0.100g/ml and 0.200g/ml group amplitude obviously reduce (P＜0.01); Compare with the 0.050g/ml group, 0.100g/ml and 0.200g/ml group amplitude reduce (P＜0.01 or P＜0.05).

With compare before the administration, the tension force of each dosage group all obviously reduces (P＜0.01 or P＜0.05); Dose-effect relationship between each dosage group not obvious (P＞0.05).

With compare before the administration, concentration is that the frequency of 0.100g/ml and 0.200g/ml group obviously reduces (P＜0.01); Compare with the 0.025g/ml group, 0.050g/m1,0.100g/ml and 0.200g/ml class frequency obviously reduce (P＜0.01 or P＜0.05); Compare with the 0.050g/ml group, 0.100g/ml and 0.200g/ml class frequency obviously reduce (P＜0.01).

6, conclusion

The Chinese medicine composition extract that contains Rhizoma Dioscoreae all has the obvious suppression effect to amplitude, tension force, the frequency that exsomatize duodenum and ileum move, and presents certain dose-effect relationship, shows that it has regulating action external to gastrointestinal motility.

Embodiment 6: the Chinese medicine composition extract that contains Rhizoma Dioscoreae is to mice gastric emptying, intestinal propulsion effect

Research

1, laboratory animal

60 of cleaning level kunming mices, male and female half and half, body weight 18～22g is provided by Zhejiang University of Traditional Chinese Medicine Animal Experimental Study center.

2, experimental drug

Described combined extracts is according to embodiment 1 preparation;

The Chinese medicine composition decoct that contains Rhizoma Dioscoreae: get prescription medical material Rhizoma Dioscoreae, Semen Coicis, Pericarpium Citri Reticulatae (2: 2: 1) in right amount, extracting in water 2 times, for the first time add 3 times of water gagings, soaked 0.5 hour, extracted 1.5 hours, add for the second time 1.5 times of water gagings, extracted 1 hour, collecting decoction filters, filtrate decompression is concentrated into the decoct of 0.8g/ml, and is standby; XIANGSHA LIUJU WAN: the Henan Province Wanxi Pharmacy Stock Co., Ltd, lot number 060602, with preceding water furnishing 0.1125g/ml suspension, standby;

Self-control is semi-solid sticks with paste: the 10g carboxymethyl cellulose, be dissolved in the 250ml distilled water, and add 16g milk powder, 8g sugar, 8g starch respectively, 2g active carbon end, stir each the adding, and the semisolid that is made into the about 300g of 300ml is stuck with paste, and deepfreeze is standby.

3, experiment equipment

Mouse stomach device, balance, graduated cylinder, the conventional operating theater instruments of dissecting: Zhejiang University of Traditional Chinese Medicine Animal Experimental Study center provides.

4, animal is selected and grouping

By the completely random design method 60 mices are divided into 6 groups, 10 every group.Be respectively: normal control group, XIANGSHA LIUJU WAN group, decoct group, the basic, normal, high dosage group of extract.The normal control group gavages equal-volume normal saline (20ml/kg), the XIANGSHA LIUJU WAN group gavages XIANGSHA LIUJU WAN (2.25g/kg), the decoct group gavages decoct (20g/kg, in contained crude drug amount), the high, medium and low dosage group of extract gavages extract 5g/kg respectively, 10g/kg, 20g/kg (in contained crude drug amount), every day 1 time, each 0.4ml.

5, experimental procedure

(1) gastric emptying experiment

With each treated animal precuring 3 days, enter formal experiment after observing no abnormal situation.Each treated animal was pressed above-mentioned dosage continuous use after 14 days, fasting be can't help water 12 hours, gavage semi-solid stick with paste (0.2ml/10g) of self-control in the 15th day every Mus, take off cervical vertebra after half an hour and put to death animal, cut the abdominal cavity open, ligation stomach cardia and pylorus, get stomach and wipe away dried back title full weight with filter paper, cut off body of stomach along greater gastric curvature then, wiping away driedly behind the flush away gastric content, claim the stomach net weight, is that the stomach residue is heavy with the difference of stomach full weight stomach function regulating net weight, the percentage ratio heavy with this and given paste is gastric food survival rate, and then draws gastric emptying rate.Computing formula is as follows:

Gastric emptying rate (%)=100%-(stomach full weight-stomach net weight) ÷ sticks with paste heavy * 100%

(2) intestinal propulsion experiment

With the digestive tract of mice in the gastric emptying experiment from pylorus until the complete extraction of rectum end, do not add traction, naturally stretch tiling, measure pylorus to ileocecus distance (small intestinal total length), and the forward position at record charcoal end is calculated the charcoal end with following formula and is advanced percentage rate to the distance (small intestinal charcoal end advances length) of pylorus.

Forward position, intestinal propulsion rate (%)=charcoal end to pylorus apart from the ÷ pylorus to ileocecus distance * 100%

6, statistical method

All (x ± S) expression carries out statistical analysis with SPSS software statistics bag to each group data to every data with mean ± standard deviation.Mean is relatively used variance analysis between many groups, relatively checks with q in twos between mean.

7, experimental result

(1) to the influence of mice gastric emptying

The result shows: compare with the normal control group, dosage group and decoct group gastric emptying rate obviously reduce (P＜0.01 or P＜0.05) in the extract; Extract high dose group gastric emptying rate compares between XIANGSHA LIUJU WAN group, each group of decoct group (P＜0.01 or P＜0.05) extract, and the high dose group gastric emptying rate is apparently higher than low dose group and middle dosage (P＜0.01 or P＜0.05).(seeing Table 3)

(2) to the propulsive influence of mouse small intestine

The result shows: compare with the normal control group, each pharmaceutical intervention group intestinal propulsion rate obviously reduces (P＜0.01 and P＜0.05); Wherein extract high dose group and XIANGSHA LIUJU WAN group intestinal propulsion rate obviously reduce (P＜0.01 and P＜0.05); Extract high dose group intestinal propulsion rate is starkly lower than decoct group (P＜0.01); Relatively, high dose group intestinal propulsion rate is starkly lower than low dose group (P＜0.05) between each group of extract.(seeing Table 3)

Table 3: each organizes the comparison of mice gastric emptying rate and intestinal propulsion rate

Annotate: compare with the normal control group: ☆ P＜0.05, ☆ ☆ P＜0.01; Compare with the XIANGSHA LIUJU WAN group: * P＜0.05; Compare with the decoct group: ★ ★ P＜0.01; Compare with the extract low dose group: ▲ P＜0.05; Compare with dosage group in the extract: ■ ■ P＜0.01.

8, conclusion

Integral experiment shows, contains that the dosage group can obviously suppress gastric emptying in the Chinese medicine composition extract of Rhizoma Dioscoreae, and high dose group can suppress intestinal propulsion, points out it can alleviate cramps of gastrointestinal tract, reduces the gastrointestinal tonicity, thereby regulates the gastrointestinal motion.

Embodiment 7: the Chinese medicine composition extract that contains Rhizoma Dioscoreae is adjusted the experimentation of mice stress ability

1, laboratory animal

120 of cleaning level Kunming mouses, body weight 20 ± 2g, male and female half and half, Zhejiang University of Traditional Chinese Medicine zoopery center provides.

2, experimental drug

The combined extracts that contains Rhizoma Dioscoreae prepares by embodiment 1, faces with preceding to be made into the filling gastric juice that concentration is respectively 0.25g/ml, 0.5g/ml, 1.0g/ml (in contained crude drug amount) with distilled water, is respectively basic, normal, high dosage medicinal liquid; The compositions decoct that contains Rhizoma Dioscoreae prepares by preparation method among the embodiment 6, faces with preceding to be made into the filling gastric juice that concentration is 2.0g/ml (in contained crude drug amount) with distilled water; XIANGSHA LIUJU WAN: the Henan Province Wanxi Pharmacy Stock Co., Ltd produces, the product colon: 050105, face with the filling gastric juice of preceding porphyrize with distilled water furnishing 0.2g/ml.Sodica calx: Shanghai the May 4th chemical reagent company limited provides production code member: Q/VAEN 1-2006.Vaseline: white clouds pharmaceutcal corporation, Ltd in Nanchang provides batch number: 20080306.

3, experiment equipment

Mouse stomach device, 250ml wide mouthed bottle, stopwatch, galvanized wire, water bath, stopwatch, electronic balance etc.

4, experimental technique

(1) animal is selected and grouping

Get 120 of cleaning level Kunming mouses, male and female half and half, body weight 20 ± 2g is divided into 6 groups at random: dosage group, extract high dose group in normal control group, XIANGSHA LIUJU WAN group, decoct group, extract low dose group, the extract.

(2) experimental procedure

The conventional raising after 3 days, each medicine group is pressed the 0.2ml/10g body weight, gavages XIANGSHA LIUJU WAN suspension, decoct, the basic, normal, high dosage medicinal liquid of extract respectively, and the normal control group gives the equivalent distilled water and irritates stomach, every day 1 time, successive administration 7 days.Behind last administration 1h, each group is got 10 mices respectively, puts it in the 250ml wide mouthed bottle that fills the 15g sodica calx (1 every bottle), smear bottleneck with vaseline, cover completely, make it air tight, pick up counting, observe and write down the time of mice because of the death of anoxia asphyxiating.In addition, 10 mices of each group residue, the bath that every Mus afterbody system puts into 20 ± 2 ℃ of depth of water 30cm, temperature with the load of body weight 10% is swum, and observes and record mice mouth is positioned at below the water surface, the dead time promptly occurs.

5, statistical method

(1) descriptive statistics: adopt the SPSS13.0 statistical software to handle.Date processing measurement data mean ± standard deviation (x ± S) expression;

(2) statistical inference: one factor analysis of variance is adopted in data statistics.Compare in twos between group, check with LSD during homogeneity of variance, check with Dunnett T3 during heterogeneity of variance.P＜0.05 is as significance of difference standard, and P＜0.01 is as difference highly significant standard.

6, experimental result

The result shows: compare with the normal control group, extraction object height, middle dosage group mice hypoxia endurance time obviously prolong (P＜0.01), and XIANGSHA LIUJU WAN group and decoct group mice hypoxia endurance time prolong (P＜0.05).(seeing Table 4)

The result shows: compare with the normal control group, extraction object height, middle dosage group, decoct group mice swimming time obviously prolong (P＜0.01), and extract low dose group and XIANGSHA LIUJU WAN group mice swimming time prolong (P＜0.05).(seeing Table 4)

Table 4: each organize mice anoxia enduring and swimming with a load attached to the body time ratio (x ± S, min:s)

Annotate: compare with the normal control group: ☆ P＜0.05, ☆ ☆ P＜0.01.

7, conclusion

The experimental result explanation, the Chinese medicine composition extract that contains Rhizoma Dioscoreae can improve the stress ability of mice, and then can infer that its preventive and therapeutic effect to FD may be to realize by the stress ability that improves body.

Embodiment 8: the experimentation that contains the Chinese medicine composition extract for preventing and treating functional dyspepsia of Rhizoma Dioscoreae

1, laboratory animal

70 of cleaning level healthy adult Wistar rats, male and female half and half, body weight 200 ± 20 grams, Zhejiang University of Traditional Chinese Medicine zoopery center provides.

2, experimental drug

The combined extracts that contains Rhizoma Dioscoreae is by embodiment 1 preparation, faces with preceding that to be made into the filling gastric juice that concentration is respectively 0.125g/ml, 0.25g/ml, 0.5g/ml (in contained crude drug amount) with distilled water be basic, normal, high dosage medicinal liquid.The compositions decoct that contains Rhizoma Dioscoreae prepares by embodiment 6 preparation methoies, faces with preceding to be made into the filling gastric juice that concentration is 1.0g/ml (in contained crude drug amount) with distilled water.XIANGSHA LIUJU WAN: the Henan Province Wanxi Pharmacy Stock Co., Ltd produces, batch number: 050105, face with the filling gastric juice of preceding porphyrize with distilled water furnishing 0.1g/ml.Folium Sennae: Chinese medicine pharmacy provides by the Zhejiang Prov. Hospital of Traditional Chinese Medicine, with 90 ℃ of hot-water soaks twice of preceding usefulness, and each 30 minutes, merge immersion, being prepared into crude drug concentration is the Folium Sennae preserved material of 0.3g/ml.

3, main experiment reagent and instrument and equipment

Vasoactive intestinal peptide (VIP) radioimmunological kit: Beijing China English biotechnology research provides lot number: 20081028; Motilin (MTL) radioimmunological kit: Beijing China English biotechnology research provides lot number: 20081028; Nitric oxide (NO) test kit, Nanjing is built up bio-engineering research and is provided, lot number: 20081028; Nitricoxide synthase (NOS) test kit, Nanjing is built up bio-engineering research and is provided, lot number: 20081028; Malonaldehyde (MDA) test kit, Nanjing is built up bio-engineering research and is provided, lot number: 20081028; Superoxide dismutase (SOD) test kit, Nanjing is built up bio-engineering research and is provided, lot number: 20081028; 0.9% sodium chloride injection, Shanghai Baxter Healthcare Ltd. provides, lot number: S0807161; 10% chloral hydrate: Zhejiang Prov. Hospital of Traditional Chinese Medicine Pharmacy department provides; 10% neutral formalin solution: Zhejiang University of Traditional Chinese Medicine zoopery center provides; R-911 is automatically put and exempted from calculating instrument: Chinese University of Science and Technology's industry head office is produced; TGL-16G type High speed refrigerated centrifuge: Anting Scientific Instrument Factory, Shanghai produces; FJ-200 high speed dispersion homogenizer: Shanghai Sample Model Factory produces; Nikon eslipse 80i microscope: Nikon company; The rotary tissue processor of STP120 type: Microm company; AP280-2 type paraffin embedding machine: Microm company; DW-FL208 type ultra cold storage freezer: U.S. Pedicellus et Pericarpium Trapae company of middle section

4, animal is selected and grouping

Get 70 of cleaning level healthy adult Wistar rats, male and female half and half, body weight 200 ± 20 grams are divided into 7 groups: normal control group, model control group, XIANGSHA LIUJU WAN group, decoct group, the basic, normal, high dosage group of extract at random.

5, animal modeling

(1) modeling method

All animals except that the normal control group are with reference to Guo Shi appropriateness folder tail stimulus method ^[4]Irritate stomach in conjunction with Folium Sennae and make liver-depression and spleen-insufficiency type FD model:,, make its rage and beat up with other rat with broken Pi Weidu with clip rat tail root place, thereby enrage full cage rat, continuously stimulate 30min at every turn, stimulate 1 time every day 4 times every 3h, continued stimulus 7 days, along with the aggravation of fighting, rat may be scratched, and disturbs for avoiding inflammation, available 0.5% povidone iodine is embrocated the injury, and control infection is disturbed to avoid inflammation.Meanwhile, every day, every rat gave Folium Sennae preserved material 2ml filling stomach, every day 2 times, and modeling 7 days, feed, drinking-water to laboratory animal during the modeling will not limit.

(2) model evaluation

Method by folder rat root of the tail portion during modeling enrages full cage rat, causes and beats up each other, and after fighting, rat forelimb performance is liftoff to be raised, and erects face-off, and send " z z z " anger, remain the rage state in half an hour.The modeling animal occurred on the 2nd day from modeling that food-intake reduces, Excreta is dirty smelly, rose in the 3rd day agitation, irritability, crissum filth to occur, the 5th day bradykinesia, like flock together, the back of a bow, there is hair color withered simultaneously, gloss reduction, and reactions such as anxiety, anxiety are arranged, food-intake obviously reduced in the 7th day.

6, pharmaceutical intervention

Duplicating the FD animal model finishes next day and plays the beginning gastric infusion, each group is pressed the 2ml/100g body weight, gavages XIANGSHA LIUJU WAN suspension, decoct, the basic, normal, high dosage medicinal liquid of extract respectively, and normal control group and model control group gavage the distilled water of equivalent, every day 2 times, successive administration 7 days.Normal feed and drinking-water during the administration.

7, observation index

(1) gross examination of skeletal muscle

Observe body weight, food ration, a quilt hair situation, activity situation, mental status and the defecation condition etc. of animal.

(2) gastric antrum histological observation

Administration after the 7th day fasting can't help water, in inferior daily 10% chloral hydrate (pressing the 4ml/kg body weight) intraperitoneal injection of anesthesia, behind the anesthetized rat stomach is opened, gastric content is rinsed well, reuse filter paper sops up the moisture on gastric mucosa top layer, gets the part gastric antrum, uses normal saline flushing immediately, the naked eyes preliminary observation has or not organic disease, as: erosion, hemorrhage etc., with pin specimen is fixed on the plastics scraps of paper then, in case it curls, 10% neutral formalin is fixed, conventional dehydration, paraffin embedding, section, HE dyeing, the general histology's performance of rat gastric antrum is respectively organized in observation under the optical microscope.

(3) to the influence of active intestinal peptide (VIP) content of gastric tissue medium vessels

Get the about 100mg of gastric tissue near pylorus, weigh and be placed in the normal saline that boils (the volume total amount of normal saline should be 9 times of piece of tissue weight) and boil 3min, add 1mol/L glacial acetic acid 0.5ml after the cooling respectively, use refiner homogenate, add 1mol NaNaOH0.5ml neutralization, 4 ℃ of centrifugal 3500r/min, 15min gets supernatant and preserves in-80 ℃ of cryogenic refrigerators.Operate according to the explanation of radioimmunity test kit, detect the content of VIP in the gastric tissue with the quantitative mode of histone.

(4) to the influence of motilin (MTL) content in the jejunum tissue

Getting the about 150mg of epimere jejunum tissue opens, content is rinsed well, reuse filter paper sops up the moisture on mucosa top layer, (the volume total amount of normal saline should be 9 times of piece of tissue weight) boils 3min in the normal saline that the back adding of weighing is boiled, add 1mol/L glacial acetic acid 0.5ml after the cooling and in homogenizer, make homogenate, the NaOH 0.5ml neutralization of reuse 1mol/L, 4 ℃ of centrifugal 3500r/min, 15min gets in the supernatant-80 ℃ cryogenic refrigerator and preserves.Operate according to the explanation of radioimmunity test kit, detect the content of MTL in the jejunum tissue with the quantitative mode of histone.

(5) to the influence of rat blood serum NO, NOS, MDA, SOD content

Give fasting the 7th day afternoon at gastric infusion and can't help water, in inferior daily 10% chloral hydrate (pressing the 4ml/kg body weight) intraperitoneal injection of anesthesia, extract 10ml blood by vein under the abdominal cavity, leave standstill more than the 2h, the centrifugal 10min of 3000r/min draws supernatant, moves in the EP pipe, seal, put in-80 ℃ of refrigerators preserve to be measured.Measure the content of serum NO level, NOS, MDA, SOD to specifications

(6) to Rats Spleen index, thymus index, the exponential influence of adrenal gland

After getting the blood agglomeration bundle, take out thymus, spleen immediately, the adrenal gland blots the blood and slime of organ surface with filter paper, uses scales/electronic balance weighing, by formula: organ index=organ weights/body weight * 1000 (unit: mg/g), calculate organ index.

8, statistical method

(1) descriptive statistics: adopt the SPSS13.0 statistical software to handle.Date processing measurement data mean ± standard deviation (x ± S) expression;

(2) statistical inference: one factor analysis of variance is adopted in data statistics.Compare in twos between group, check with LSD during homogeneity of variance, check with Dunnett T3 during heterogeneity of variance.P＜0.05 is as significance of difference standard, and P＜0.01 is as difference highly significant standard.

9, experimental result

(1) gross examination of skeletal muscle

Rats in normal control group diet, activity, stool character and hair etc. are all no abnormal, and body weight increases gradually; Model control group rat appearance activity reduces, and hair is withered, the back of a bow, and body weight obviously descends, and appetite reduces, and happiness flocks together, symptoms such as loose stool; General symptom of extract high, medium and low three groups and decoct group, XIANGSHA LIUJU WAN group rat and symptom of digestive tract are along with treatment has in various degree recovery.

(2) gastric antrum histological observation

Each treated animal gastric antrum of perusal is organized does not all have organic diseases such as rotten to the corn, hemorrhage.Observed result shows under the optical microscope, and the gastric antrum mucosa is methodically arranged, and the mucosal epithelium cell is arranged closely, N/D, and no ulcer, the lamina propria body of gland is not seen atrophy.

(3) to liver-depression and spleen-insufficiency type FD rat stomach intestinal hormones, oxygen radical removing ability and Immune Effects

Compare with the normal control group, the content of VIP rising (P＜0.05) in the model control group rat stomach tissue, the content of MTL reduces (P＜0.05), serum NO content, NOS vigor, MAD content significantly raise (P＜0.01), the SOD vigor significantly reduces (P＜0.01), and index and spleen index obviously reduces (P＜0.05).Thymus index and adrenal gland's index differential there are no significant meaning (P＞0.05).

Compare with model control group, the content of each dosage group of combined extracts and XIANGSHA LIUJU WAN group VIP obviously reduces (P＜0.01 or P＜0.05), the content of combined extracts, decoct and XIANGSHA LIUJU WAN group MTL obviously raise (P＜0.01 or P＜0.05), NO content, NOS vigor, MDA content reduce (P＜0.01 or P＜0.05), SOD vigor rising (P＜0.01 or P＜0.05), index and spleen index raises (P＜0.05), and each index is all comparatively remarkable with middle dosage group in the combined extracts.

Compare the content rising (P＜0.01 or P＜0.05) of low, the middle dosage group of extract MTL with the XIANGSHA LIUJU WAN group.

Compare with the decoct group, the content of dosage group MTL obviously raises (P＜0.01) in the extract, and NO content obviously reduces (P＜0.01).(seeing Table 5)

10, conclusion

The Chinese medicine composition extract that contains Rhizoma Dioscoreae can obviously improve the various symptoms of liver-depression and spleen-insufficiency type FD rat, improve its jejunum tissue MT L content, reduce gastric tissue VIP content, reduce serum NO levels, NOS vigor, show that it can effectively regulate the gut hormone of liver-depression and spleen-insufficiency type FD rat parasecretion, promote digestive tract power, improve the FD symptom that delayed gastric emptying causes.Simultaneously, can obviously reduce spleen liver depression-type FD rat blood serum MDA content, improve the SOD vigor, thereby improve the ability that body is removed oxygen-derived free radicals, suppress lipid peroxidation in the gastrointestinal tissue, reducing a large amount of generations of oxygen-derived free radicals in the body, alleviate the histiocyte extent of damage, may be one of action pathway of its control FD.Also can improve spleen liver depression-type FD Rats Spleen ponderal index, point out it can increase FD rat immunity organ weight, strengthen lymphocyte function, thereby have the effect of adjusting immune function.

Claims (5)

1. Chinese medicine composition that contains Rhizoma Dioscoreae, form by the component that the quality proportioning is following:

5～25 parts of Rhizoma Dioscoreaes

5～25 parts of Semen Coiciss

1～15 part of Pericarpium Citri Reticulatae.

2. Chinese medicine composition as claimed in claim 1 is characterized in that described composition quality proportioning is as follows:

10 parts of Rhizoma Dioscoreaes

10 parts of Semen Coiciss

5 parts of Pericarpium Citri Reticulataes.

3. the extracting method of Chinese medicine composition according to claim 1, it is characterized in that described method comprises: Rhizoma Dioscoreae, Semen Coicis and the Pericarpium Citri Reticulatae of getting formula ratio, the adding quality is the water of Rhizoma Dioscoreae, Semen Coicis and 1.5～5 times of quality of Pericarpium Citri Reticulatae gross mass, reflux, extract, 1～3 time, each 0.5～2 hour, merge aqueous extract, filter, get filtrate decompression concentrate clear paste, ethanol to the ethanol final concentration that clear paste stirs adding 80～95% is 60～70%, leave standstill more than 24 hours, get supernatant, cross the leaching decompression filtrate recycling ethanol, concentrate, get Chinese medicine extract.

4. the application of Chinese medicine composition as claimed in claim 1 in preparation control functional dyspepsia medicine.

5. application as claimed in claim 4 is characterized in that described medicine makes one of following dosage form: 1. capsule, 2. granule, 3. powder, 4. tablet.