CN101715725B - Method for removing impurities and maintaining purity for fields of shandan 8806 maize hybrid seeds - Google Patents
Method for removing impurities and maintaining purity for fields of shandan 8806 maize hybrid seeds Download PDFInfo
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- CN101715725B CN101715725B CN2009102195499A CN200910219549A CN101715725B CN 101715725 B CN101715725 B CN 101715725B CN 2009102195499 A CN2009102195499 A CN 2009102195499A CN 200910219549 A CN200910219549 A CN 200910219549A CN 101715725 B CN101715725 B CN 101715725B
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Abstract
The invention discloses a method for removing impurities and maintaining purity for fields of shandan 8806 maize hybrid seeds, which selects a maize inbred line L102 as a female parent and a maize inbred line K22 as a male parent, and produces the maize hybrid seed shandan 8806 according to the conventional method; when the maize hybrid seedlings shandan 8806 are cultured between the three-leaf stage and the five-leaf stage, weeding is performed in the maize fields, a herbicide nicosulfuron or a mixed herbicide containing nicosulfuron is selected to be sprayed on field weeds and stem leaves of the shandan 8806 hybrid seedlings to remove the female parent inbred line plants, then thinning and final singling of seedlings are performed according to the conditions of the field seedlings. The method can realize weeding in the maize fields as well as maintaining purity of the hybrid seeds, thus ensuring the field purity of the maize hybrid seed shandan 8806 and providing a simple, intuitive and accurate purity detection method for the maize hybrid seed shandan 8806.
Description
Technical field
The invention belongs to crop heterosis and utilize technical field, pure method is protected in the field removal of impurities that is specifically related to single 8806 corn hybrid seeds in a kind of Shan.
Background technology
Corn is to utilize hybrid vigour one of crops the most widely the earliest in the agricultural production.For a long time, utilize the hybrid vigour of crop to become important measures that increase corn yield and improve quality.Usually, the production of corn hybrid seed can obtain through following two kinds of approach:
(1) male method is taken out in manual work;
(2) cytoplasm male sterility three line method etc.
Yet in the middle of the actual production of corn hybrid seed; Because a variety of causes; People can not totally drain the maternal male flower of corn inbred line only completely in time, perhaps can not reach sterile degree fully because of reasons such as weather cause the male sterile degree of maternal inbred line sometimes, cause the corn seed purity of production can not reach 100%; Thereby influenced the heterotic utilization of corn variety, caused the reduction of corn hybrid seed output or a large amount of corn seed to scrap because of purity problem.
Corn seed is that Maize Production basically the most also is the means of production of most critical. the particularly increase of maternal self-crossing seedling in the corn hybrid seed of the reduction of purity, its can significantly reduce maize yield and quality.About corn hybrid seed seed purity and the research of field corn relation of yield more.For example, Li Chengde [crop magazine, 2004 (2) 46～48] research is thought: extremely significant growth and decline relation is arranged between purity of hybrid maize and the field corn output.Maternal inbred line strain rate is in 0.67%～16% scope, and the every raising 1% of purity can increase production 0.4751%, but the per hectare units increased in production reaches 40.25kg.Red beautiful No. 6 corn purity Combined Trials that six units such as Dandong Inst. of Agricultural Science carried out in 1978 show, the seed of purity 94.6% is than the seed underproduction 14.9% of purity 99.66%, 67.8 kilograms of the mu underproduction; The result that the corn seed purity that units such as Agricultural University Of Shenyang carried out for No. 3 with Shen Dan in 1981 is tested yield effect is: the seed of purity 96.5%, 95.6% is than the seed underproduction 10.5% and 11.1% respectively of purity 98.6%; Academy of agricultural sciences, Shandong corn research institute served as for No. 4 that the test that the examination material carries out has also drawn similar result with No. 2, middle list, the former list in Shandong in 1980.Test shows, No. 10 corn seeds of the iron list of purity 94% are than No. 10 corn kinds of iron list of purity 99%, 67.5 kilograms of every mu of underproduction.Although the research of various places constituent parts is different because of the kind of being selected for use, the result who obtains is not quite identical, and any is arranged is similar, and promptly the corn hybrid seed seed purity affects the output of corn crop significantly.In the middle of the actual Maize Production of China, the production loss that causes because of purity is low is very surprising, therefore should give great attention.
Single 8806 corn hybrid seeds in Shan are to adopt self bred inbred line L102 to make female parent by agricultural college of Xibei Univ. of Agricultural & Forest Science & Technology, and K22 does the paternal hybrid assembly and forms.2005 through Henan Province's kind introduction and Experiment (Henan draws beautiful 2005015) through the authorization of the Shaanxi Province variety certification committee (Shan examines beautiful 2003004) in 2003.This kind obtained national new variety of plant protection power in 2007, its kind power number is CNA20030271.X.Single 8806 corn hybrid seeds in Shan have high yield, stable yields, characteristics such as protect green property, fecundity is good and resistant to lodging and adaptability is strong.
In China, the weed killer herbicide kind that is used to prevent and kill off corn field weed at present is more, but has that the grass of killing is effective, environmental safety strong and operation is used easily medicament seldom.Find herbicide nicosulfuron (nicosulfuron), chemical name: 2-(4 under study for action; 6-dimethoxypyridin-2-base carbamyl aminosulfonyl)-and N, the N-dimethyl nicotinamide is a systemic herbicide; Can be weeds cauline leaf and root and absorb, in plant corpus, conduct subsequently, cause sensitive plant growth retardation, cauline leaf chlorisis, withered gradually; Death in 20-25 days generally speaking, but under the lower situation of temperature, some perennial weed is needed the long time.In the dispenser good drug efficacy before the phase of 4 leaves behind the bud, the dispenser drug effect descended when seedling was big.This medical instrument has the preceding activity of weeding of bud, but active in low behind the bud.Herbicide nicosulfuron has the following advantages:
(1) biologically active is high, and application dosage is few.Every mu of effective ingredient consumption is the 2.3-4.7 gram only, and activity is tens times of existing conventional dose, and is reliable to corn and Environmental security;
(2) preventive effect is good, can kill corn field weed and reach more than 90%, can effectively prevent and treat annual and perennial grassy weed and some broad-leaved type weeds;
(3) selectivity is strong, and the residence time is short, and is minimum to the influence of succession crop and environment;
(4) toxicity is low, and no carcinogenic, teratogenesis, mutagenesis belong to low-toxin farm chemicals;
(5) operation is used conveniently, it can either seedling before the sealing weeding, but again behind the seedling cauline leaf spray.
Just because of all advantages of nicosulfuron, make nicosulfuron become the most widely used a kind of sulfonylurea herbicide of present corn field.
Summary of the invention
Pure method is protected in the field removal of impurities that the purpose of this invention is to provide single 8806 corn hybrid seeds in a kind of Shan; This method can make the field purity of single 8806 corn hybrid seeds in Shan increase considerably; To improve corn hybrid use of advantage level; Keep the intrinsic yield potentiality of corn variety, reduce corn variety because of the reduction of purity problem to output.
In order to realize above-mentioned task, the present invention realizes through following approach:
Pure method is protected in the single 8806 corn hybrid seed field removal of impurities in a kind of Shan, it is characterized in that this method comprises the following steps:
1) it is maternal to select corn inbred line L102 to do, and corn inbred line K22 makes male parent, produces corn hybrid seed Shan single 8806 by conventional method hybridization;
2) when the single 8806 hybridization seedlings in corn Shan grow to three leaves wholeheartedly to one heart stage of five leaves; In conjunction with the corn field weeding; The mixed herbicide of selecting herbicide nicosulfuron or containing nicosulfuron is sprayed to weeds in field and the cauline leaf that seedlings are hybridized in Shan single 8806; Remove maternal inbred line plant, carry out the thinning final singling according to the field growth of cereal crop seedlings then.
The described mixed herbicide that contains nicosulfuron is meant the complex preparation of nicosulfuron and other weed killer herbicide, and wherein the mu consumption of pure nicosulfuron is no less than 3.0 grams.
Described thinning final singling is implemented after using herbicide nicosulfuron or containing 7～8 days of mixed herbicide of nicosulfuron.
The present invention has the following advantages and beneficial effect:
(1) can guarantee and improve the singly field purity of 8806 corn hybrid seeds of Shan, keep the intrinsic yield potentiality of corn variety, improve corn hybrid use of advantage level, and then avoid the production loss of corn land for growing field crops.
(2) utilize this method also can a kind of simple, intuitive and accurate purity check method be provided to single 8806 corn hybrid seeds in Shan.
(3) under the situation of not increasing input, in cornfield weeding, also can realize the pure purpose of crossbreed removal of impurities guarantor.
Embodiment
The applicant is through discovering; Different corn inbred lines exists extremely significant difference in seedling stage to the resistance of herbicide nicosulfuron; For example, some corn inbred line has the sensitive lethality proterties to herbicide nicosulfuron, and some corn inbred line then has good resistance to herbicide nicosulfuron; Utilize this otherness; The applicant selects the corn inbred line L102 that weed killer herbicide is had the sensitive lethality proterties to make female parent, and the corn inbred line K22 that weed killer herbicide is had good resistance makes male parent, hybridizes single 8806 corn hybrid seeds in production Shan according to the method for routine then.
The further experimental study of applicant is also found; Corn inbred line L102 belongs to recessive character to the sensitive lethality proterties of herbicide nicosulfuron; It and the hybrid F1 generation that the corn inbred line K22 hybridization that herbicide nicosulfuron is had resistance produces have good resistance equally to herbicide nicosulfuron.
Therefore, pure method is protected in the field removal of impurities of single 8806 corn hybrid seeds in Shan of the present invention, selects the inbred line L102 that herbicide nicosulfuron is had the sensitive lethality proterties to do maternal; Its corn inbred line K22 with resistance is made male parent; Produce corn hybrid seed according to the method for routine, when the corn hybridization seedling is grown to one heart stage of three leaves to one heart stage of five leaves, in conjunction with the corn field weeding; Select herbicide nicosulfuron or contain the mixed herbicide of nicosulfuron; Cauline leaf to the single 8806 hybridization seedlings of weeds in field and Shan is sprayed, and removes maternal inbred line plant, carries out the thinning final singling according to the field growth of cereal crop seedlings after 7～8 days.
At herbicide nicosulfuron or contain in the mixed herbicide application of nicosulfuron, be sure not to mix use, if be necessary that its medication interval phase is no less than 7 days with organophosphorous pesticide.
Below be the embodiment that the inventor provides, the invention is not restricted to these embodiment.In following examples, the constituent content of weed killer herbicide and land for growing field crops spraying concentration are all used according to weed killer herbicide distributors recommended amounts.
Embodiment 1:
Be chosen in and seedling stage the inbred line L102 that herbicide nicosulfuron has the sensitive lethality proterties made female parent, its corn inbred line K22 with good resistance is made male parent, produce corn hybrid seed Shan single 8806 according to the method for routine.
When seedlings growth to three leaves are hybridized wholeheartedly to five leaves one heart stage (prioritization scheme is one heart stages of three leaves) in corn Shan single 8806; In conjunction with the corn field weeding; Employing contains nicosulfuron mixed herbicide (suspending agent; Wherein nicosulfuron content 2.5%, atrazine content 19.5%), every mu of dose by 120 grams carries out cauline leaf to single 8806 milpas of weeds and Shan comprehensively and sprays.
Because maternal inbred line L102 plant does not have resistance to herbicide nicosulfuron, it can stop growing after dispenser, 4～5 days top young leaves chlorosis, and leaf sheath and vein and partial blade all become aubergine after 7～8 days, and whole strain is dead gradually.Herbicide nicosulfuron does not have obvious influence to single 8806 plant in corn hybrid seed Shan, can carry out the thinning final singling according to the field growth of cereal crop seedlings then, like this; Both can the little individual plant of abnormal aubergine be removed; Also can it be regarded as undesired assorted strain and keep when the thinning final singling, the last undesired little assorted strain of aubergine is dead automatically, thereby has correctly eliminated corn inbred line L102 plant; Improved corn hybrid seed field purity; Empirical tests, the purity of single 8806 corn hybrid seeds in the Shan of results brings up to 99.8%, has given full play to the intrinsic yield potentiality of corn hybrid seed.
Embodiment 2:
Be chosen in and seedling stage the inbred line L102 that herbicide nicosulfuron has the sensitive lethality proterties made female parent, its corn inbred line K22 with good resistance is made male parent produce corn hybrid seed Shan single 8806 according to the method for routine.
When seedlings growth to three leaves are hybridized wholeheartedly to five leaves one heart stage (prioritization scheme is one heart stages of three leaves) in corn Shan single 8806; In conjunction with the corn field weeding; With nicosulfuron herbicide agent (oil-suspending agent; Effective component content be 40 gram nicosulfurons/liter), according to every mu 70～100 gram consumption weeds and milpa are carried out cauline leaf comprehensively and spray.
Because maternal inbred line L102 plant does not have resistance to herbicide nicosulfuron, it can stop growing after dispenser, 4～5 days top young leaves chlorosis, and leaf sheath and vein and partial blade all become aubergine after 7～8 days, and whole strain is dead gradually.Herbicide nicosulfuron does not have obvious influence to single 8806 plant in corn hybrid seed Shan; Can carry out the thinning final singling according to the field growth of cereal crop seedlings then, both can the little individual plant of abnormal aubergine be pulled out, also can it be regarded as undesired assorted strain and keep when the thinning final singling; The last undesired little assorted strain of aubergine is dead automatically; Thereby correctly eliminate corn inbred line L102 plant, improved the field purity in corn hybrid seed Shan single 8806, empirical tests; The purity of single 8806 corn hybrid seeds in the Shan of results brings up to 99.9%, has brought into play the intrinsic yield potentiality of corn hybrid seed.
Claims (2)
1. pure method is protected in the single 8806 corn hybrid seed field removal of impurities in Shan, it is characterized in that this method comprises the following steps:
1) it is maternal to select corn inbred line L102 to do, and corn inbred line K22 makes male parent, and corn hybrid seed Shan single 8806 is produced in hybridization;
2) grow to three leaves wholeheartedly to five leaves during one heart stage when the single 8806 hybridization seedlings in corn Shan; In conjunction with the corn field weeding; The mixed herbicide of selecting herbicide nicosulfuron or containing nicosulfuron is sprayed to weeds in field and the cauline leaf that seedlings are hybridized in Shan single 8806; Remove maternal inbred line plant, carry out the thinning final singling according to the field growth of cereal crop seedlings then, described thinning final singling is implemented after using herbicide nicosulfuron or containing 7～8 days of mixed herbicide of nicosulfuron.
2. the method for claim 1 is characterized in that, the described mixed herbicide that contains nicosulfuron is meant the complex preparation of nicosulfuron and other weed killer herbicide, and wherein the mu consumption of pure nicosulfuron is no less than 3.0 grams.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009102195499A CN101715725B (en) | 2009-12-17 | 2009-12-17 | Method for removing impurities and maintaining purity for fields of shandan 8806 maize hybrid seeds |
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| CN2009102195499A CN101715725B (en) | 2009-12-17 | 2009-12-17 | Method for removing impurities and maintaining purity for fields of shandan 8806 maize hybrid seeds |
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| CN101715725A CN101715725A (en) | 2010-06-02 |
| CN101715725B true CN101715725B (en) | 2012-02-01 |
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| CN2009102195499A Expired - Fee Related CN101715725B (en) | 2009-12-17 | 2009-12-17 | Method for removing impurities and maintaining purity for fields of shandan 8806 maize hybrid seeds |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102550395B (en) * | 2012-01-12 | 2013-06-26 | 西北农林科技大学 | Field weeding and purity-keeping method for corn hybrids |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1217870A (en) * | 1997-11-28 | 1999-06-02 | 湖北省农业科学院农业现代化研究所 | Technology for making male sterile of female lines for rice hybridization |
| CN1253472A (en) * | 1997-04-28 | 2000-05-17 | 严文贵 | Crop heterosis and herbicide |
| CN1263701A (en) * | 1999-01-27 | 2000-08-23 | 黄大年 | Breeding method for hybridized rice |
| CN1914982A (en) * | 2006-09-05 | 2007-02-21 | 张家口市农业科学院 | Method for planting millet cenospecies of anti weedicide |
| US20090275473A1 (en) * | 2008-05-02 | 2009-11-05 | Pioneer Hi-Bred Interntional, Inc. | Chemical selection of resistant gametes of plants in the field |
-
2009
- 2009-12-17 CN CN2009102195499A patent/CN101715725B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1253472A (en) * | 1997-04-28 | 2000-05-17 | 严文贵 | Crop heterosis and herbicide |
| CN1217870A (en) * | 1997-11-28 | 1999-06-02 | 湖北省农业科学院农业现代化研究所 | Technology for making male sterile of female lines for rice hybridization |
| CN1263701A (en) * | 1999-01-27 | 2000-08-23 | 黄大年 | Breeding method for hybridized rice |
| CN1914982A (en) * | 2006-09-05 | 2007-02-21 | 张家口市农业科学院 | Method for planting millet cenospecies of anti weedicide |
| US20090275473A1 (en) * | 2008-05-02 | 2009-11-05 | Pioneer Hi-Bred Interntional, Inc. | Chemical selection of resistant gametes of plants in the field |
Non-Patent Citations (2)
| Title |
|---|
| 苏少泉."玉米田除草剂新品种与应用".《农药研究与应用》.2009,第13卷(第1期),第1－6页. |
| 苏少泉."玉米田除草剂新品种与应用".《农药研究与应用》.2009,第13卷(第1期),第1-6页. * |
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