Embodiment
In order to make those skilled in the art person understand the present invention better, and above-mentioned purpose of the present invention, feature and advantage can be become apparent more, the present invention is further detailed explanation below in conjunction with embodiment,
Embodiment 1:
For examination worm source: 2 committee's exigua larvaes that the field collects in July, 2010, carry out artificial feeding indoor, obtain adult and with lay eggs and raise to testing the required length of time.
Test method is as follows:
Stomach toxicity toxicity test method: adopt leaf dipping method to carry out the stomach toxicity toxicity test.The three-coloured amaranth blade soaked 10 ~ 15s in soup after, taking-up is dried, and is that the card punch of 1cm is made the roundleaf sheet, put into 12 orifice plates with diameter, healthy 2 neat ages and 4 ages 1 in 2 committee every holes of exigua larvae are chosen, 30 larvas of every processing in 3 in every hole, repeat for 3 times, handle in contrast with clear water.Place 26 ℃ of insulating boxs to raise each processing, statistics examination worm death toll behind the 24h.
The working concentration of various medicaments is the recommended density of commodity, and when composite, the final concentration of two kinds of medicaments is half of concentration when using separately.
The gained lethality is proofreaied and correct with the Abbott formula:
Result of the test
As can be seen from Table 1, in the common insecticide for examination, different medicaments are to the drug effect difference of 2 committee noctuids, the larva pesticide resistance significant difference in the different length of times.From larva stomach toxicity effect, the second instar larvae drug effect reached has chlopyrifos (94.44%), phoxim (93.33%), Methomyl (91.67%), an emamectin-benzoate (93.33%) more than 80%; And the four-age larva pesticide resistance significantly improves, wherein the drug effect of emamectin-benzoate quickly falls to 58.89%, drug effect is still having (86.67%) two kind of organophosphorous pesticide of chlopyrifos (88.89%) phoxim and a Methomyl (83.33%) more than 80%; It is all not good to the drug effect in 2 the different worm attitude of committee noctuid length of times to test used pyrethrin pesticide, but Methomyl and efficient cypermethrin compounded use can significantly improve the stomach toxicity virulence to 2 noctuids of entrusting, and can reach 94.44% to the lethality rate of 4 instar larvaes; The composite use of Methomyl and emamectin-benzoate also has good drug effect (92.22%) to 4 instar larvaes.This shows, two select the committee noctuid to the organophosphorous pesticide sensitivity, in non-organophosphorous pesticide, Methomyl is all better to the larva stomach toxicity effect in the different length of times, be the efficient medicament of control 2 committees noctuid, in addition, although pyrethrin pesticide and emamectin-benzoate are relatively poor to 2 committee's noctuid 4 instar larvae virulence, but with the composite use of Methomyl, can significantly improve drug effect.
In summary, Methomyl can be used as the agricultural chemicals of 2 committees of control noctuids, and can be used alternatingly with phoxim, chlopyrifos etc. for 2 committees of control noctuids with pyrethrin pesticide and emamectin-benzoate are composite, slows down drug-fast generation; Or the replacement organophosphorous pesticide, use the same period with weed killer herbicide, reach the double effects of weeding and insect protected.
Table 1 different insecticides is to the drug effect of 2 committee's exigua larvaes
Annotate: data are mean value ± standard error in the table, and different letter representations are significant difference on 5% level;
Embodiment 2
Test site: the Zang Cun of Ningjin County, Xingtai.
Kind: the summer corn experimental cultivar is Zheng Dan 958.
Test period: on June 30th, 2011.
Test plot soil is loam, middle fertility.
Test method: the mode dispenser of spraying is adopted in this test, needs to remove the maize seedling residual body of wheat straw on every side before the dispenser on wheat stubble between the ridge; Mu soup 45kg, concrete dosage is handled as follows:
A: mu 60mL 77.5% DDT EC;
B: mu 60mL 40% phoxim emulsifiable concentrate;
C: mu 60mL 20% methomyl milk oil;
D: mu 30mL 20% methomyl milk oil+10mL 2% emamectin-benzoate missible oil;
E: mu 30mL 20% methomyl milk oil+20mL 4.5% r;
G: clear water is handled
Every processing residential quarter 100m
2, repeating 3 times, after the dispenser second day, each treatment region all used nicosulfuron to carry out weeding.
Control time and method: before the medication, in the test region, the removing field is all pulled out in all dead killed strains of wilting, staying all is not killed healthy plant.After the hazard boom, each residential quarter plant is carried out general census, record killed strain number respectively, floral leaf poisoning strain number calculates killed strain rate, control efficiency and poisoning rate.
Result and analysis
The different medicaments of table 2 and nicosulfuron use the preventive effect of 2 committee noctuids simultaneously and the poisoning situation occurs
Test is handled | Total strain number | Killed strain number | Killed strain rate % | Preventive effect % | Floral leaf strain number | Poisoning rate % |
A | 662.67 | 19.67 | 2.97 | 84.31 | 537.67 | 81.14 |
B | 668.33 | 22.33 | 3.34 | 81.29 | 551.33 | 82.49 |
C | 676.00 | 22.67 | 3.35 | 81.23 | 2.33 | 0.34 |
D | 672.33 | 12.00 | 1.78 | 90.01 | 1.00 | 0.15 |
E | 661.67 | 14.67 | 2.22 | 87.59 | 2.00 | 0.30 |
F | 671.33 | 127.00 | 18.92 | ? | 1.33 | 0.20 |
Annotate: data are the mean value of 3 repetitions in the table
As can be seen from Table 2, under conventional amount used, it is all better to the control efficiency of 2 committee noctuids to test used several medicaments, the composite use of Methomyl and first dimension salt, beta-cypermethrin, and control efficiency is more quite a lot of; Cause used corn field to use herbicide nicosulfuron always in second day, and dichlorvos and phoxim are used the field and serious poisoning all occurred, and the poisoning rate is more than 80%, and other field pieces almost do not have poisoning to occur.
Implementation column 3
Test site: the Zang Cun of Ningjin County, Xingtai.
Kind: the summer corn experimental cultivar is Zheng Dan 958.
Test period: on July 2nd, 2011.
Test plot soil is loam, middle fertility.
Test method: 3 kinds of chemicals treatment modes are established in this test altogether, are respectively spraying, irritate root and spread poison bait, need to remove the maize seedling residual body of wheat straw on every side before the dispenser on wheat stubble between the ridge; The processing mode of poison bait is mu wheat bran with 5kg stir-fry perfume, converts suitable quantity of water to moistening, adds medicament, and abundant mixing is sprinkling upon the maize seedling limit in suitable ridge of dusk; Every chemicals treatment mode is established 5 concentration, mu soup 45kg, and concrete dosage sees Table 2; Every processing residential quarter 100m
2, repeat 3 times
Control time and method: before the medication, in the test region, the removing field is all pulled out in all dead killed strains of wilting, staying all is not killed healthy plant.Behind test region medication 24h, 72h and 168h, each residential quarter plant is carried out general census respectively, record killed strain number respectively, calculate killed strain rate and control efficiency.
The different application methods of table 3 and the control efficiency (168h) of different dosages to 2 committee noctuids
Annotate: data are the mean value of 3 repetitions in the table
Result and analysis
As can be seen from Table 3, different dispenser processing modes are along with the increase of formulation rate (concentration), and control efficiency also increases gradually.Spraying is handled, and spraying medicine concentration is between 500 ~ 2000 times the time, and preventive effect raises along with the increase of concentration, and when concentration was 500 times, preventive effect was preferably 92.86%; Root irrigation, similar with the spraying result, spraying medicine concentration is between 500 ~ 2000 times the time, and preventive effect raises along with the increase of concentration, and when concentration was 500 times, preventive effect was preferably 93.75%; Spread poison bait and handle, the mu formulation rate is between 75mL ~ 175mL the time, and preventive effect raises along with the increase of formulation rate, and when formulation rate was 175mL, preventive effect was preferably 94.30%.
Embodiment 4
Test site: the Zang Cun of Ningjin County, Xingtai.
Kind: the summer corn experimental cultivar is Zheng Dan 958.
Test period: on July 5th, 2011.
Test plot soil is loam, middle fertility.
Test method: mu is copied fragrant wheat bran with 5kg, converts suitable quantity of water to moistening, adds and respectively handles medicament, and abundant mixing is in being sprinkling upon the maize seedling limit along the ridge at dusk.Concrete processing is as follows:
A: mu 125mL 20% methomyl milk oil;
B: mu 100mL 20% methomyl milk oil+10mL 2% emamectin-benzoate missible oil (active ingredient weight ratio 100:1);
C: mu 75mL 20% methomyl milk oil+20mL 2% emamectin-benzoate missible oil (active ingredient weight ratio 37.5:1);
D: mu 50mL 20% methomyl milk oil+30mL 2% emamectin-benzoate missible oil (active ingredient weight ratio 16.67:1);
E: mu 25mL 20% methomyl milk oil+40mL 2% emamectin-benzoate missible oil (active ingredient weight ratio 6.25:1);
F: mu 50mL2% emamectin-benzoate missible oil
G: be left intact
Every processing residential quarter 100m
2, repeat 3 times; Investigation method and time are with embodiment 2.
Result and analysis:
Table 4 Methomyl and emamectin-benzoate different proportion are mixed to the preventive effect of 2 committee noctuids
Test is handled | Total strain number | Killed strain number | Killed strain rate | Preventive effect % |
A | 681.00 | 31.00 | 4.55 | 77.81 |
B | 676.00 | 30.33 | 4.49 | 78.13 |
C | 685.33 | 19.00 | 2.77 | 86.48 |
D | 672.67 | 10.67 | 1.59 | 92.27 |
E | 671.33 | 26.33 | 3.92 | 80.88 |
F | 689.00 | 29.67 | 4.31 | 79.01 |
G | 677.67 | 139.00 | 20.51 | — |
Annotate: data are the mean value of 3 repetitions in the table
As can be seen from Table 4, with being mixed for 2 committees of control exigua larvae of emamectin-benzoate and Methomyl different proportion, the gained control efficiency has evident difference.Wherein 20% methomyl milk oil and 2% emamectin-benzoate missible oil amount ratio are between 16.67 ~ 37.5:1) time in 75:20 ~ 50:30(active ingredient weight ratio, the control efficiency that is mixed all is significantly higher than the control efficiency that each medicament uses separately, both amount ratios are 16.67:1 in 50:30(active ingredient weight ratio) time effect best, preventive effect is up to 92.27%.
Embodiment 5
Test site: the Zang Cun of Ningjin County, Xingtai.
Kind: the summer corn experimental cultivar is Zheng Dan 958.
Test period: on July 5th, 2011.
Test plot soil is loam, middle fertility.
Test method: mu is copied fragrant wheat bran with 5Kg, converts suitable quantity of water to moistening, adds and respectively handles medicament, and abundant mixing is in being sprinkling upon the maize seedling limit along the ridge at dusk.Concrete processing is as follows:
A: mu 125mL 20% methomyl milk oil;
B: mu 100mL 20% methomyl milk oil+30mL 4.5% r (active ingredient weight ratio 14.81:1);
C: mu 75mL 20% methomyl milk oil+60mL 4.5% r (active ingredient weight ratio 5.56:1);
D: mu 50mL 20% methomyl milk oil+90mL 4.5% r (active ingredient weight ratio 2.47:1);
E: mu 25mL 20% methomyl milk oil+120mL 4.5% r (active ingredient weight ratio 0.93:1);
F: mu 150mL 4.5% r
G: be left intact
Every processing residential quarter 140m
2, repeat 3 times; Investigation method and time are with embodiment 2.
Result and analysis:
Table 5 Methomyl and beta-cypermethrin different proportion are mixed to the preventive effect of 2 committee noctuids
Test is handled | Total strain number | Killed strain number | Killed strain rate | Preventive effect % |
A | 918.67 | 36.67 | 3.99 | 78.90 |
B | 927.00 | 33.00 | 3.56 | 81.19 |
C | 919.00 | 14.67 | 1.60 | 91.59 |
D | 922.33 | 26.00 | 2.82 | 85.11 |
E | 930.00 | 41.00 | 4.41 | 76.69 |
F | 926.33 | 86.67 | 9.36 | 50.48 |
G | 917.67 | 173.33 | 18.89 | — |
Annotate: data are the mean value of 3 repetitions in the table
As can be seen from Table 5, with being mixed for 2 committees of control exigua larvae of Methomyl and beta-cypermethrin different proportion, the gained control efficiency has evident difference.Wherein 20% methomyl milk oil and 4.5% beta-cypermethrin amount ratio are between 2.47 ~ 5.56:1) time in 50:90 ~ 75:60(active ingredient weight ratio, the control efficiency that is mixed all is significantly higher than the control efficiency that each medicament uses separately, both amount ratios are 5.56:1 in 75:60(active ingredient weight ratio) time effect best, preventive effect is up to 91.59%.
Methomyl be a kind of in absorption have tag, the broad spectrum pesticide of the carbamates of stomach poison function, it has better control efficiency to 2 committee's exigua larvaes in summary, to notice when using in the field that being mixed of agricultural chemicals use and rotate use, with effective pest control, delay the generation of pest resistance to insecticide.
A kind of new purposes of 2 committees of Methomyl control noctuid of utilizing that the present invention relates to is described by specific embodiment.Those skilled in the art can use for reference links such as content appropriate change raw material of the present invention, process conditions and realize corresponding other purpose, its relevant change does not all break away from content of the present invention, all similar replacements and change will become apparent to those skilled in the art that and all be regarded as comprising within the scope of the present invention.