CN101700101A

CN101700101A - Zinc-containing microbial feed additive and fabrication method thereof

Info

Publication number: CN101700101A
Application number: CN200910172673A
Authority: CN
Inventors: 黄建壮
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-11-24
Filing date: 2009-11-24
Publication date: 2010-05-05

Abstract

The invention relates to a zinc-containing microbial feed additive capable of improving metabolism of animal bodies and a fabrication method thereof. The fabrication method is importantly characterized in that an organic zinc compound is added in culture solution containing soybean cake hydrolyzate and corn steep liquor, and then bifidobacterium, lactobacillus plantarum and pseudomonas are inoculated for carrying out liquid fermentation. With the progression of the fermentation, the mass propagation of the added strains is carried out, and the added organic zinc is gradually transformed into an organic zinc protein, thereby preparing a zinc-containing liquid protein product. Then, wheat bran and other carriers are further added, and a yeast strain is inoculated for carrying out solid fermentation, thereby preparing a solid product. The product contains a large number of beneficial live bacterial prepration, beneficial enzyme preparation, glutathione and zinc-rich protein, and can significantly improve the metabolism of animals, promote the healthy rapid growth of the animals, simultaneously significantly improve the reproduction rate, the egg laying rate and the milk production rate of the animals and improve the anti-disease ability of the animals as the microbial feed additive.

Description

A kind of Zinc-containing microbial feed additive and preparation method thereof

1. technical field

The present invention relates to livestock and poultry and use microbial forage additive field, specifically a kind of Zinc-containing microbial feed additive and preparation method thereof.After the product that utilizes this method to make enters in the animal body such as milk cow, can play and promote digestion, promotion absorbs, promotes the metabolic effect of animal to zinc, also can play vegetative cell simultaneously, prevent cell impaired, make up the interior good micro-ecological environments of animal body such as milk cow, promote the absorption of nutriment to transform, improve immunity of organisms, promote animal health to grow fast, can also significantly improve animal reproduction rate, laying rate, the rate of giving milk, and the raising of animal disease resistant power is had effect.

2. technical background

Zinc is that humans and animals carries out the requisite a kind of trace element of normal activities, and especially a lot of important enzyme in the human body all needs the participation of zinc could synthesize and guarantee normally carrying out of metabolism.The expert of United States Department of Agriculture current research report is pointed out, absorbs enough zinc, can health has flexibly, the performance of energy.The researcher carries out the diet research in nine weeks by a definite date at the male sex sportsman of 12 two teens, allows experimental group absorb the zinc of 18 micrograms every day, and control group has only the zinc of 3 micrograms every day.The researcher points out, when the zinc picked-up is not enough, just can't discharge the carbon dioxide that accumulates in the health effectively, also can't allow oxygen exchange fully in vivo, makes completely long-pending metabolic refuse in the health, naturally will be more and more tired.China people's diet structure is with the cereal master, and the bioavailability of zinc is very low in cereal, is 20-40% only, if children eat purified diet more, wherein the content of zinc is lost too much, the easier zinc dificiency that causes.

Each system of whole body all can be adversely affected during zinc deficiency.Especially to puberty gonad maturity influence more direct.The lad of The Nile, Egypt delta suffers from when lacking zinc disease, and main performance is growth retardation and hypogonadism.Confirm after deliberation that in Iran maiden and school-ager have growth retardation and the slow person of sexal maturity, food is strengthened zinc after, patient is grown is tending towards normal.Zoopery confirms, lacks the animal brain depauperation of zinc.Some tissue of eyeball lacks zinc, will influence photochemistry, makes eyesight become undesired.Zinc is again the composition of insulin, and the zinc in the pancreas is reduced to a half of normal contents, and the possibility of diabetes is just arranged.All have the zinc of denier to distribute in the overwhelming majority tissue, wherein liver, muscle and bone content are higher.Zinc is the main component of tens of kinds of enzymes in the body.Zinc is also relevant with brain development and intelligence.University of the U.S. finds that the interior zinc content of teenager's body clever, that succeed in school is all than fathead height.Zinc promotes the effect of lymphopoiesis and mobility in addition, cutaneous lesions such as epithelium and mucosal tissue are normal to keeping, defense against bacterial, viral intrusion, promotion wound healing, minimizing acne, and the malfunctioning grade of the correction sense of taste all has magical effect.

3. summary of the invention

In view of the important function of zinc, the invention provides and a kind ofly can improve metabolic Zinc-containing microbial feed additive of animal body and preparation method thereof for the humans and animals vital movement.After the product that utilizes this method to make enters in the animal body such as milk cow, can play and promote digestion, promotion absorbs, promotes the metabolic effect of animal to zinc, make up the interior good micro-ecological environments of animal body such as milk cow, promote the absorption of nutriment to transform, improve immunity of organisms, promote animal health to grow fast.Its key character is at first to add organic zinc compound in the nutrient solution that contains soya-bean cake hydrolyzate and corn steep liquor, inserts Bifidobacterium, Lactobacillus plantarum, false monospore bacillus again and carries out liquid fermentation.Along with the carrying out of fermentation, the bacterial classification of adding is bred in a large number, the organic zinc that adds is also gradated be organic zinc albumen, promptly makes the liquid protein product that contains zinc.And then add carrier such as wheat bran and insert the saccharomycete bacterial classification and carry out solid fermentation, make solid product.Contain a large amount of useful active bacteria formulations, useful enzyme preparation, glutathione in this invention product and contain the abundant albumen of zinc, can significantly improve the metabolism of animal as microbial forage additive, promote animal health to grow fast, simultaneously can also significantly improve animal reproduction rate, laying rate, the rate of giving milk, and the raising of animal disease resistant power is had effect.

For achieving the above object, a large amount of useful active bacteria formulation of the present invention comprises Bifidobacterium, Lactobacillus plantarum, false monospore bacillus and saccharomycete and metabolin; Active biological enzyme comprises proteolytic enzyme, amylase, contains zinc digestive ferment, superoxide dismutase, cellulase; Contain zymoprotein, zinc methionine that participation protein active division center that zinc albumen forms for the organic zinc that adds transforms forms after microbial metabolism, contain the compound of formation such as zinc polypeptide.

For achieving the above object, preparation method of the present invention, its key character is:

(1) soya-bean cake hydrolyzate, corn steep liquor and sterilized water are according to percentage (10-15): (3-5): (80-87) be configured to fluid nutrient medium earlier, carry out the high-temperature boiling sterilization;

(2) in will the fluid nutrient medium packing round behind boiling sterilization, and seal after adding the organic zinc material of percentage (0.5-1.0);

(3) get the preferred bacterial classification of cultivating that is in logarithmic phase and inoculate, Bifidobacterium, Lactobacillus plantarum, false monospore bacillus bacterium liquid is precentagewise (10-15) respectively: (5-10): (75-85) add.Adjusting the pH value is (7.0-7.5), and temperature is (25-35 a ℃), Continuous Cultivation (6-8) sky;

(4) after the end of liquid fermentation stage, liquid fermentation material and wheat bran are (through high-temperature sterilization, and spreading for cooling to room temperature) add according to the ratio of percentage by weight (1: 1.5), insert saccharomycete bacterium liquid (1-4) % simultaneously and carry out solid fermentation, incubation time be (2-5) sky;

(5) the solid fermentation stage mainly is to utilize wheat bran to contain zinc microbial liquid product to make solid product as carrier absorption.Can be observed after solid fermentation finishes and be covered with the white hypha body in the material, after dehydrating, can be made into and contain zinc microbial solid formulation products.

4. advantage of the present invention

(1) zinc in this product can participate in the synthetic of the interior plurality of enzymes of animal body, helps metabolism and normally carries out and can promote growing

(2) keep the normal sense of taste of animal and appetite, regulate V _AMetabolism

(3) can promote lymphocytic division and cell to change, keep the immunologic function of cell

(4) promote protein synthetic, quicken cell division and breeding, but and stabilized cell membrane structure

(5) power of regeneration of enhancing wound tissue is accelerated reparation, the healing of wound tissue.

Claims

1. a kind of Zinc-containing microbial feed additive of the present invention, its key character are that it contains a large amount of useful active bacteria formulations, useful enzyme preparation and contain the abundant albumen of zinc; Useful active bacteria formulation wherein comprises Bifidobacterium, Lactobacillus plantarum, false monospore bacillus and saccharomycete and metabolin; Active biological enzyme comprises proteolytic enzyme, amylase, contains zinc digestive ferment, superoxide dismutase, cellulase; Contain zymoprotein, zinc methionine that participation protein active division center that zinc albumen forms for the organic zinc that adds transforms forms after microbial metabolism, contain the compound of formation such as zinc polypeptide.

2. additive for microbe feedstuff as claimed in claim 1, it is characterized in that the living bacterial cells sum in the product reaches 2,000,000,000/ml-40, hundred million/ml, based on Bifidobacterium, Lactobacillus plantarum, it accounts for total viable count (65-85) %, and false monospore bacillus and saccharomycete account for (10-15) % of total viable count respectively.

3. additive for microbe feedstuff as claimed in claim 1 is characterized in that the addition in the feed is (0.1-0.5) % of feed gross weight, and the addition in drinking-water is (0.01-0.03) % of water weight.

4. the preparation method of a kind of Zinc-containing microbial feed additive of the present invention, it is characterized in that at first in the nutrient solution that contains soya-bean cake hydrolyzate and corn steep liquor, adding organic zinc compound, insert Bifidobacterium, Lactobacillus plantarum, false monospore bacillus again and carry out liquid fermentation.Along with the carrying out of fermentation, the bacterial classification of adding is bred in a large number, the organic zinc that adds is also gradated be organic zinc albumen, promptly makes the liquid protein product that contains zinc.And then add carrier such as wheat bran and insert the saccharomycete bacterial classification and carry out solid fermentation, make solid product.