CN101686722B - Composition including Chlorella vulgaris and extract and uses - Google Patents
Composition including Chlorella vulgaris and extract and uses Download PDFInfo
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- CN101686722B CN101686722B CN2008800165844A CN200880016584A CN101686722B CN 101686722 B CN101686722 B CN 101686722B CN 2008800165844 A CN2008800165844 A CN 2008800165844A CN 200880016584 A CN200880016584 A CN 200880016584A CN 101686722 B CN101686722 B CN 101686722B
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- ginseng
- chlorella
- valerian
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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Abstract
The present invention relates to a novel health food comprising chlorella extract, a herbal extract obtained from a mixture of herbs selected from Panax ginseng, Ganoderma lucidum, Valeriana frauriei birq, Poria cocus, Zerumbet Bupleurum scorzoneraefolium, Glycyrrhiza and along with a malt and honey. The health food has the enhancement effects of anti-oxidant, immunoenhancing, autonerve-modulating and hematopoiesis.
Description
Technical field
The present invention relates to a kind of chlorella extract that comprises, the herb extracts that is obtained by the herbal mixture that is selected from ginseng (Panax ginseng), glossy ganoderma (Ganoderma lucidum), valerian (Valeriana frauriei birq), Poria cocos (Poria cocus), rhizoma zingiberis (Zerumbet), radix bupleuri (Bupleurum scorzoneraefolium), Radix Glycyrrhizae (Glycyrrhiza), and the new health food of Fructus Hordei Germinatus and honey.That this health food has is anti-oxidant, strengthen the function of immunity, regulating plant nervous system, hematopoiesis.
Background technology
In order to improve immune system, the health food of having developed many kinds of chemicals and having comprised the immunopotentiator of plant origin at present.Known to numerous, immune system is activated by Castle's intrinsic factor and extrinsic factor or suppresses.As if the factor of Immunosuppression system is a lot, such as oxidative stress, immune cell function obstacle, automatic nervous system disorder, hematopoietic disorder, trophic disturbance etc.
Oxidative stress is exactly that the free radical of superoxides and so on is caused, and this superoxides is to produce by human body metabolism, various diseases, radioactive ray etc.
Free radical is the normal accessory substance in the daily metabolism, and it promotes disease and aging course.Free radical seems Promote immunity system and cytoclasis.Especially free radical is considered to possibility Immunosuppression cell or macrophage, and macrophage plays main key effect in immune response.Antioxidant can resist radical effect, so improving immunocompetence.
In the middle of the people that suffer from inhibitive ability of immunity disease or similar symptom, lymphocyte quantity reduces, and cell factor quantity also correspondingly reduces.Cell factor plays an important role in immune system.
Some scientists prompting, immune system and automatic nervous system substantial connection.Find by in overwork, grave illness or the virus infections generation body stress the time, stomodaeal nervous system is by excitement, and parasympathetic is suppressed, thereby can produce insomnia, neuroticism and gastricism.Continue the weakening that such state brings immune defense function.
When the endogenous immune response was not enough, the inhibition of stomodaeal nervous system was considered to can be used for strengthening immune response, and the hyperfunction of parasympathetic can strengthen immune response.Immunostimulant, hematopoiesis promoter, nutriment etc. can strengthen immune defense.
Many medicines have useful effect to the human immune system, nowadays also come out but can regulate antioxidation, immune stimulatory cell, enhancing hematopoiesis function and neuro medicinal herbs extract.
Main purpose of the present invention is exactly that exploitation strengthens the health food of immune defense function with treatment or prevention various diseases.
Described immunomodulator, material and nutritious supplementary pharmaceutical that hematopoiesis is relevant strengthen immune defense.
Prepared health food of the present invention to reduce oxidative stress, activating immune cell, regulating plant nervous system and to strengthen hemopoietic system.
Health food of the present invention comprises chlorella extract, the herb extracts and Fructus Hordei Germinatus and the honey that are obtained by the herbal mixture that is selected from ginseng, glossy ganoderma, valerian, Poria cocos, rhizoma zingiberis, radix bupleuri, Radix Glycyrrhizae.
Summary of the invention
Chlorella (being called high nutritional agents) is the monoplast green alga class, and it contains polysaccharide, polysaccharide mixture and active glucan, and is used as immunomodulator.Chlorella also is rich in vitamin, mineral matter, dietary fibre, nucleic acid and amino acid.
Ginseng stimulates the immunocyte of secrete cytokines, thereby strengthens immune response.Ginseng promotes fatigue recovery, prevents haemolysis and promotes hematopoiesis.It also has hyperfunction, the system that excites nerve, cardiac stimulant, excitant function, digests and assimilates and anti-allergic effects.It also has antioxidation.
Glossy ganoderma contains ergosterol, protease, several amino acids, organic acid and polysaccharide.Known glossy ganoderma has a significant effect to calmness, spinal cord, prostate, autoimmune disease, hematopoiesis function, intestines and stomach, liver, small intestine and large intestine.Glucan in the glossy ganoderma and lentinan can be used for improving immunologic function, and as antiblastic.
The glycocalix in chlorella, ginseng, glossy ganoderma source is thought the activated immune cell agent in the existing document.The present invention seeks to make the immunostimulatory activity of above-mentioned polysaccharide to be conducive to treat various diseases.
Valerian can be used for improving palpitaition, nervousness, insomnia (agrypnia) and heart disease, and these effects are caused by the lipid components in the valerian.
Poria cocos contains the pachyman (pakaman) that belongs in a large number polysaccharide, and this composition has insomnia (aryypina) and sedation
Rhizoma zingiberis contains volatile oil, fat sample material, thus rhizoma zingiberis can be used as aromatic series stomach invigorating antiemetic be used for the treatment of have a stomach-ache, vomiting, the vomiting of pregnancy, indigestion.
Radix bupleuri has brings down a fever and sedation, therefore can be used for treating malaria, cold, intercostal neuralgia, neurasthenia, anxiety, and also is used for the treatment of hepatitis, cholecystitis and cholepathia.This effect
Radix Glycyrrhizae long ago just is used for the treatment of other diseases, such as ulcer, productive cough, and be used for detoxifcation, seasoning, and main active ingredient is glycyrrhizin.
Notice that at present the several mineral materials in the Fructus Hordei Germinatus and forulic acid (perula acids) have significant antioxidation activity and sympatho inhibitory activity.
About treatment cranial vascular disease, respiratory disease, disease of digestive system, disease of the genitourinary system, disease of metabolism, hepatitis, tuberculosis, gynecologic and obstetric disease, vegetative nerve disease and climacteric the various diseases such as obstacle, this health food demonstrates excellent effect.
The specific embodiment
Health food of the present invention comprises chlorella extract, the herb extracts and Fructus Hordei Germinatus and the honey that are obtained by the herbal mixture that is selected from ginseng, glossy ganoderma, valerian, Poria cocos, rhizoma zingiberis, radix bupleuri, Radix Glycyrrhizae.
In a specific embodiments of the present invention, the content of chlorella extract is at about 5mg-50mg, in the preferred 10mg-20mg scope.
In a specific embodiments of the present invention, ginseng is in about 14-17% scope of described health product.
In a specific embodiments of the present invention, glossy ganoderma is in about 13-17% scope of described health product.
In a specific embodiments of the present invention, valerian is in about 12-18% scope of described health product.
In a specific embodiments of the present invention, Poria cocos is in about 10-15% scope of described health product.
In a specific embodiments of the present invention, rhizoma zingiberis is in about 10-13% scope of described health product.
In a specific embodiments of the present invention, radix bupleuri is in about 5-9% scope of described health product.
In a specific embodiments of the present invention, Radix Glycyrrhizae is in about 6-7% scope of described health product.
In one or more specific embodiments of the present invention, the percentage of Fructus Hordei Germinatus is in about 8-10% scope.
In another specific embodiments of the present invention, the characteristic that the composition of this health food has is anti-oxidant, automatic nervous system is regulated, immunity strengthens and hematopoiesis function promotes.
The present invention also provides has that anti-oxidant, automatic nervous system is regulated, immunity strengthens and hematopoiesis function promotes the preparation method of the health food of characteristic.
Specify the present invention below by embodiment.
Embodiment 1: the preparation of the present composition
1. the preparation of Aquo-composition of the present invention
Chlorella extract;
Chlorella dry powder is dissolved in the ethyl acetate, extracted 10 hours at 45 ℃.
Centrifugal solution is obtained supernatant.
Ethanol is incorporated in the supernatant, the precipitation active material.
Filtering precipitate is cleaned the vacuum drying extract with absolute ethyl alcohol.
Herb extracts;
Ginseng, glossy ganoderma, valerian, Poria cocos, rhizoma zingiberis, radix bupleuri, Radix Glycyrrhizae are mixed and be crushed to size is about 0.001-10mm
3
The mixture of pulverizing is added in distilled water or the ethanol, extracted 5 hours at 80 ℃.
The solution of centrifugal extraction is obtained supernatant.
Fructus Hordei Germinatus;
Behind the boiling rice bran, aspergillus oryzae (Aspergillus Oryzae) is implanted.
20-25 ℃ of fermentation 2-3 days,
Certainly degraded about 10 hours at 50-80 ℃,
Distilled water is added in degraded solutions,
Centrifugal mixed solution is obtained supernatant.
Mix
To mix by chlorella extract, malt extract and the herb extracts that above-mentioned steps obtains, again honey is added in the composition of the present invention.
2. the preparation of solid composite of the present invention
1) ginseng, glossy ganoderma, valerian, Poria cocos, rhizoma zingiberis, radix bupleuri, Radix Glycyrrhizae are mixed and pulverizing.
2) mixture of pulverizing is added in the distilled water, extracted 24 hours at 60 ℃.
3) filtering the solution that extracts also concentrates.
4) concentrated material is mixed with chlorella extract and malt extract, and then together rub with honey and to mix.
5) this is rubbed the mixture of mixing and make pill.
According to the method described above, the present composition with and each component content shown in table 1, table 2, table 3 and table 4.
Table 1: the present composition 1
Composition | %w/w |
Chlorella | 0.01 |
Ginseng | 16 |
Glossy ganoderma | 15 |
Valerian | 13 |
Poria cocos | 12 |
Rhizoma zingiberis | 10 |
Radix bupleuri | 9 |
Radix Glycyrrhizae | 7 |
Fructus Hordei Germinatus | 8 |
Proper honey extremely | 100 |
[0065] Table 2: the present composition 2
Composition | %w/w |
Chlorella | 0.013 |
Ginseng | 14 |
Glossy ganoderma | 16 |
Valerian | 12 |
Poria cocos | 15 |
Rhizoma zingiberis | 11 |
Radix bupleuri | 9 |
Radix Glycyrrhizae | 6 |
Fructus Hordei Germinatus | 9 |
Proper honey extremely | 100 |
Table 3: the present composition 3
Composition | %w/w |
Chlorella | 0.015 |
Ginseng | 17 |
Glossy ganoderma | 13 |
Valerian | 18 |
Poria cocos | 10 |
Rhizoma zingiberis | 12 |
Radix bupleuri | 8 |
Radix Glycyrrhizae | 7 |
Fructus Hordei Germinatus | 9 |
Proper honey extremely | 100 |
[0069] Table 4: the present composition 4
Composition | %w/w |
Chlorella | 0.02 |
Ginseng | 16 |
Glossy ganoderma | 17 |
Valerian | 15 |
Poria cocos | 12 |
Rhizoma zingiberis | 13 |
Radix bupleuri | 5 |
Radix Glycyrrhizae | 7 |
Proper honey | 8 |
*Mg is the chlorella extract that adds, and is not weight ratio
Embodiment 2: the mensuration of antioxidation activity
The elimination of DPPH free radical can cause the DPPH change color, so Antioxidative Activity Determination is as follows:
5mg DPPH is dissolved in 60% methyl alcohol of 100ml with preparation DPPH solution.
Sample solution: DPPH solution (750ul) and mixed solution UC(200ul).
Blank solution: the mixed solution of 60% methyl alcohol (750ul) and UC solution (200ul).
Contrast solution: the mixed solution of DPPH solution (750ul) and 60% methyl alcohol (200ul).
Under 515nm, measure respectively the absorbance of each solution.Antioxidation activity (free radical elimination activity) calculates with following formula:
Antioxidation activity (%)=(C-S)/(C-B) * 100
C: contrast solution absorbance
B: blank solution absorbance
C: sample solution absorbance
Above-mentioned steps is applied to respectively the present composition 1, the present composition 2, the present composition 3 or the present composition 4.Antioxidation activity is as shown in table 5 below.
Table 5: the antioxidation activity of the present composition
The present composition | 1 | 2 | 3 | 4 |
Antioxidation activity | 82 | 87 | 83 | 85 |
Embodiment 3: the mensuration of hematopoietic potential
Radiation destroys bone marrow cell, and generation and the propagation of normal immunocyte is had harmful effect, has therefore reduced hematopoiesis and immunologic function.Explore administration UC in this experiment and can reduce the hematopoiesis of following radiotherapy and the reduction of immunologic function.
Mouse (body weight is 24-27g) is with 60Co, the illuminated gamma-rays of 2Gy.After the irradiation with UC2 with 0.3ml/kg oral 30 days.Kill respectively mouse in irradiation after 10 days, 20 days and 30 days, then extract peripheral blood, measure the wherein quantity of red blood cell (RBC), leucocyte (WBC) and blood platelet (BP).
Table 6: red blood cell number changes (* 10
12/ l) (n=30)
Table 7: platelet count changes (* 10
9/ l) (n=30)
Table 8: leukocyte count changes (* 10
9/ l) (n=30)
Expression relative ratios in ().
* represent that the contrast group has significant difference (p<0.05)
* represents that the contrast group has significant difference (p<0.01)
Test bit represents mean+/-standard error.
Embodiment 4: to neuro regulating action
In this test, UC1 measures by the method for changes in heart rate inspection regulating neuro impact.
The patient was lied on a bed 5-10 minute, then electrocardiogram II (ECG II) conductor is arranged on patient's the four limbs.ECG data was input into computer 5 minutes continuously.
LF/HF is than the reflection sympathetic nerve function.
The UC2 of 0.3ml/kg is administered orally in the patient of insomnia, and administration was measured LF/HF according to said method after 2 hours.
Table 9: on the impact (n=5) of automatic nervous system adjusting
From above-mentioned table as seen, composition of the present invention has the effect that suppresses stomodaeal nervous system and hyperfunction parasympathetic.
Embodiment 5: the cytophagic impact of human peripheral blood
PBS (contrast) or UC are injected in mouse peritoneum.After 24 hours, put to death mouse, extract peripheral blood in heparin solution (5IV/ml) from eyeball.
Carry out the phagocytosis test of yeast cream behind the counting.To add through the new blood 0.1ml that heparin is processed the yeast cream (5 * 10 of 0.05ml dilution
8/ ml) in, 37 ℃ of lower incubations 60 minutes, gentle agitation once in a while.Incubation is coated cell suspending liquid on the slide of light microscope after finishing.Carry out Accustain dyeing, use observation by light microscope.
The cell of engulfing minimum 3 particles is thought positive cell.The result is as shown in table 6.The result clearly illustrates that, but present composition intense stimulus monocyte and granulocyte phagocytosis.
Table 10: the monocyte of engulfing and granulocytic percentage
Embodiment 10: the present composition is on the impact of engulfing of the cell that is derived from the abdominal cavity
With PBS (contrast) and UC intraperitoneal injection in one group of mouse.After 24 hours, put to death mouse, abdominal cavity cell is collected in the culture medium.Behind the hemocytometer counting cells, in the culture medium that contains 5% hyclone, abdominal cavity cell is diluted to 1 * 10
7Cell.
To contain 2 * 10 in the culture medium of 5% hyclone at 0.2ml
6Cell and isopyknic yeast cream (5 * 10
8/ ml) be mixed together.Then 37 ℃ of lower incubations 60 minutes, stir once in a while.Under 150g, stopped incubation in centrifugal 5 minutes, sediment is suspended again.
Under light microscope, estimate the macrophage of Phagocytic granules in the smear after the Accustain dyeing.Engulf 4 or more granose cell and be considered to positive.The result is as shown in table 7, and the result shows that composition of the present invention can stimulate the phagocytosis of peritoneal macrophages consumingly.
Table 11: the percentage of the macrophage of engulfing
Claims (8)
1. composition, it is comprised of following material: chlorella extract; The concentrated herb extracts that the mixture that is comprised of ginseng, glossy ganoderma, valerian, Poria cocos, rhizoma zingiberis, radix bupleuri, Radix Glycyrrhizae obtains; Malt extract and honey, described malt extract is aspergillus oryzae to be implanted Fructus Hordei Germinatus matrix obtain, and the raw material of described composition is: chlorella 0.01wt%, ginseng 16wt%, glossy ganoderma 15wt%, valerian 13wt%, Poria cocos 12wt%, rhizoma zingiberis 10wt%, radix bupleuri 9wt%, Radix Glycyrrhizae 7wt%, Fructus Hordei Germinatus 8wt%, honey surplus.
2. composition, it is comprised of following material: chlorella extract; The concentrated herb extracts that the mixture that is comprised of ginseng, glossy ganoderma, valerian, Poria cocos, rhizoma zingiberis, radix bupleuri, Radix Glycyrrhizae obtains; Malt extract and honey, described malt extract is aspergillus oryzae to be implanted Fructus Hordei Germinatus matrix obtain, and the raw material of described composition is: chlorella 0.013wt%, ginseng 14wt%, glossy ganoderma 16wt%, valerian 12wt%, Poria cocos 15wt%, rhizoma zingiberis 11wt%, radix bupleuri 9wt%, Radix Glycyrrhizae 6wt%, Fructus Hordei Germinatus 9wt%, honey surplus.
3. composition, it is comprised of following material: chlorella extract; The concentrated herb extracts that the mixture that is comprised of ginseng, glossy ganoderma, valerian, Poria cocos, rhizoma zingiberis, radix bupleuri, Radix Glycyrrhizae obtains; Malt extract and honey, described malt extract is aspergillus oryzae to be implanted Fructus Hordei Germinatus matrix obtain, and the raw material of described composition is: chlorella 0.015wt%, ginseng 17wt%, glossy ganoderma 13wt%, valerian 18wt%, Poria cocos 10wt%, rhizoma zingiberis 12wt%, radix bupleuri 8wt%, Radix Glycyrrhizae 7wt%, Fructus Hordei Germinatus 9wt%, honey surplus.
4. each described composition of claim 1-3, it exists with tablet and solution form.
5. each described composition of claim 1-3 has application in the health food of antioxidation activity in preparation.
6. each described composition of claim 1-3 has application in the health food that promotes hematopoiesis function in preparation.
7. each described composition of claim 1-3 has unify the application in the health food of hyperfunction parasympathetic effect of lax sympathetic nervous system in preparation.
8. each described composition of claim 1-3 has the application in the health food of immune defense function of radiation in preparation.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KP07373 | 2007-05-18 | ||
KP37307 | 2007-05-18 | ||
KP07-373 | 2007-05-18 | ||
PCT/KP2008/000012 WO2008143365A1 (en) | 2007-05-18 | 2008-01-21 | Composition containing chlorella and herbal extracts |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101686722A CN101686722A (en) | 2010-03-31 |
CN101686722B true CN101686722B (en) | 2013-04-24 |
Family
ID=40032042
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN2008800165844A Expired - Fee Related CN101686722B (en) | 2007-05-18 | 2008-01-21 | Composition including Chlorella vulgaris and extract and uses |
Country Status (2)
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CN (1) | CN101686722B (en) |
WO (1) | WO2008143365A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101999725B (en) * | 2010-10-20 | 2012-10-03 | 汤永强 | Method for preparing chlorella pyrenoidosa nucleic acid organic drink |
IT202000019789A1 (en) * | 2020-08-07 | 2022-02-07 | Kolinpharma S P A | MULTICOMPONENT COMPOSITION INCLUDING GANODERMA LUCIDUM EXTRACT, PANAX EXTRACT, VITAMINS, ZINC AND BACTERIA STRAINS AND ITS USE IN THE PREVENTION AND TREATMENT OF INFLUENZA SYMPTOMS AND IN INCREASING THE IMMUNE DEFENSES |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1112131C (en) * | 2000-01-07 | 2003-06-25 | 华南理工大学 | Green alga food |
DE10062813A1 (en) * | 2000-12-18 | 2002-07-11 | Katharina Kern | Nutritional supplement based on migroalgae, also containing medicinal plant to give stronger and more targeted action, e.g. in improving general health or improving blood flow |
DE102006042149A1 (en) * | 2005-11-18 | 2007-05-24 | Christov, Bozena, Dr. | Home animal fodder product made from dried fabric parts enriched with auxiliary agents, where the nutritional value is increased by inserting the fabric parts into a saturated solution with water and the auxiliary agents before drying |
KR100715653B1 (en) * | 2006-04-21 | 2007-05-09 | 박승만 | Growth-promoting foodstuff |
CN100391488C (en) * | 2006-05-26 | 2008-06-04 | 李继业 | Chinese medicinal health product |
-
2008
- 2008-01-21 CN CN2008800165844A patent/CN101686722B/en not_active Expired - Fee Related
- 2008-01-21 WO PCT/KP2008/000012 patent/WO2008143365A1/en active Application Filing
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Publication number | Publication date |
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WO2008143365A1 (en) | 2008-11-27 |
CN101686722A (en) | 2010-03-31 |
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