CN101675740A - Repair plant black nightshade seed-soaking solution and application thereof - Google Patents
Repair plant black nightshade seed-soaking solution and application thereof Download PDFInfo
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- CN101675740A CN101675740A CN 200810013230 CN200810013230A CN101675740A CN 101675740 A CN101675740 A CN 101675740A CN 200810013230 CN200810013230 CN 200810013230 CN 200810013230 A CN200810013230 A CN 200810013230A CN 101675740 A CN101675740 A CN 101675740A
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Abstract
The invention relates to a quick germination method of heavy metal repair plant black nightshade seed, concretely a repair plant-black nightshade seed-soaking solution and application thereof. The seed-soaking solution using water as solvent comprises: A single seed-soaking fluid: 0.1-0.3 weight% of hydrogen peroxide; or B, a mixed seed-soaking fluid: 0.1-0.3 weight% of hydrogen peroxide, 100-300g/L gibberellin, 0.1-0.3 weight% of KNO3 and 0.01-0.1 weight% of KMnO4. The germination time of the black nightshade seed is obviously shortened and the germination rate is obviously increased and theplant young seedling is stronger.
Description
Technical field
The present invention relates to the quick germinating method of heavy metal repair plant black nightshade seed, specifically a kind of repair plant black nightshade seed-soaking solution and application thereof.
Background technology
The phytoremediation of heavy-metal contaminated soil mainly is meant utilizes heavy metal accumulation plant (particularly super enriching plant) excess from contaminated soil to absorb one or more heavy metals, and with its transfer, be stored to overground part organs such as stem, leaf, subsequently with plant integral body (comprising the part root) results and concentrated the processing, and then plantation continuously, so that make heavy metal in soil concentration be reduced to the acceptable level.[document 1:Chaney R.L., Malik M., Li Y.M., et al.1997, Phytoremediation of soilmetals.Current Opinions in Biotechnology.8:279～284; Document 2:Wong M.H., 2003, Ecological restoration of mine degraded soils, with emphasis on metalcontaminated soils.Chemosphere.50:775～780].
Black nightshade is a kind of cadmium super enriching plant of finding recently, cadmium pollution soil had higher reparative potential (document 3:Wei S.H., Zhou Q., X.Wang X., Zhang K.S., Guo G.L., MaL.Q.2005, A newly-di scovered Cd-hyperaccumulator Solanum nigrum L.Chinese Science Bulletin.50 (1): 33-38).There are some researches show that stem and leaf are the major organs that black nightshade is extracted Cd, flowering stage, axis, leaf dry weight sum reached 93.4% of maturing stage stem, leaf dry weight sum respectively.Account for to the maturing stage in flowering stage in 63.2% time of the whole potted plant growth time of plant, seed only accounts for whole plant to 8.3% of Cd clearance to the clearance of Cd.And flowering stage two, the plant species overground part reached the maturing stage respectively to 87.5% of Cd recovery rate to the recovery rate of Cd.If employing is gathered in the crops plant in flowering stage and will be used for the extraction reparation of next batch plant flowering stage to the maturing stage during this period of time, the remediation efficiency of plant will be single 1.75 times of (document 4:Wei S.H. that plant one batch of plant, .Zhou Q.X., Koval P.V.2006.Flowering stage characteri st ics of cadmiumhyperaccumulator Solanum nigrum L.and their significance tophytoremediation.Science of the Total Environment.369:441-446), this will greatly improve phytoremediation efficient.But the precondition that realizes this purpose is to take measures to shorten the seedling-growing time of black nightshade, and therefore, it is very crucial promoting black nightshade to germinate and improve germination rate fast.
Though about the more existing reports of the high-yield culture technique of black nightshade and the rule rate of growing (document 5: Wang Yiming, the pests occurrence rule of jungle .1995. black nightshade and the characteristic research preliminary study that grows. weeds science .2:13-14; Document 6: Liu Liang .2000. black nightshade artificial planting high yield test. traditional Chinese medicine .23 (10): 594-595; Document 7: Bian Yong, indoor growing and the utilization of Lu's winter rosy clouds .2003. black nightshade. biology magazine .20 (4): 40-41), but yet there are no report about its quick germinating method, this also is the problem that phytoremediation technology presses for solution.
Summary of the invention
The objective of the invention is to by adopting seed-soaking liquid so that a kind of quick germinating method of repair plant black nightshade to be provided the germinating time of its seed significantly be shortened, germination rate significantly improves, and plant seedlings is also relatively more healthy and stronger simultaneously.
For achieving the above object, the technical solution used in the present invention is:
Repair plant black nightshade seed-soaking solution is that the seed-soaking liquid of solvent consists of with water,
A. single seed-soaking liquid, mass concentration 0.1%～0.3% hydrogen peroxide;
Or B. mixing seed-soaking liquid, mass concentration 0.1%～0.3% hydrogen peroxide, 100～300g/L gibberellin, mass concentration 0.1%～0.3%KNO
3With mass concentration 0.01～0.1%KMnO
4
The application of described seed-soaking liquid in the repair plant black nightshade fast breeding:
1) black nightshade seed is all used mass concentration 1-2%NaClO solution disinfection 5-15 minute before seed soaking, and distilled water flushing is clean and shrivelled kernel floated remove, and is standby behind the natural air drying.
2) seed soaking:
A. single seed-soaking method is promptly sowed without cleaning directly after 12 hours with the seed soaking of mass concentration 0.1%～0.3% hydrogen peroxide;
B. mix seed-soaking method, promptly use quality 0.1%～0.3% hydrogen peroxide, 100～300g/L gibberellin, 0.1%～0.3%KNO
3With 0.01～0.1%KMnO
4The mixed liquor seed soaking was sowed without cleaning directly after 12 hours.
Above-mentioned two kinds of methods are all carried out under illumination condition, and light application time is 8-17.5 hour, and intensity of illumination is 350～450umolm
-2s
-1, temperature is between 25～30 ℃.
3) sowing and breeding: in temperature 25-30 ℃, air humidity: under 50%～80% condition, black nightshade seed is seeded in the soil thickness of earth covering 1-2cm on the seed; Soil moisture content is 60～80% of a maxmun field capacity, and controll day, light application time was 8～17.5 hours, can emerge by nature in 8-10 days.
Described soil: pH value 6.5～7.5, the content of organic matter 14.3～20.5g/kg, full N content 0.5～1.1g/kg, full P content 0.5～0.9g/kg, effective P content 8-15mg/kg, quick-acting K content 70～95mg/kg.
The present invention has following advantage:
1. the seedling-growing time of black nightshade seed shortens greatly.Adopt the present invention can make the seedling-growing time of black nightshade seed become 8-10 days by 2 weeks (the about 15-16 of conventional cultivation seedling-growing time days), shortened near half.
2. germination rate and emergence rate significantly improve.Adopt the present invention can make the germination rate of black nightshade seed reach 84.7-96.7%, emergence rate reaches more than the 81.6-96.1%.
3. seedling is relatively more healthy and stronger, and its leaf look and growing way all are better than the seedling of handling without seed-soaking liquid.
Embodiment
The germination test of embodiment 1 black nightshade seed
1.1 materials and methods
1.1.1 material
Black nightshade seed is collected in ecological experiment station, Chinese Academy of Sciences Shenyang.Gather ripe Solanum nigrum fruit, seed is cleaned with running water and natural air drying after standby.Chemical reagent potassium nitrate, potassium permanganate, hydrogen peroxide, gibberellin are common agents on the market.Why select above-mentioned 4 kinds of seed-soaking liquids for use, be because: gibberellin has the effect of antagonism inhibiting substances abscisic acid (ABA); Potassium permanganate and hydrogen peroxide (H
2O
2) be strong oxidizer, can promote the degraded and the discharge with inner noxious material of entering of oxygen, be beneficial to the normal redox reaction of cell interior; K in the potassium nitrate improves the activity that promotes the metabolism plurality of enzymes to a certain extent as the activator of plurality of enzymes, also makes cell membrane obtain the part reparation, therefore, all to germinateing facilitation is arranged all.
1.1.2 test method
1) black nightshade seed is all used mass concentration 1-2%NaClO solution disinfection 5-15 minute before seed soaking, and distilled water flushing is clean and shrivelled kernel floated remove, and is standby behind the natural air drying.
2) seed soaking:
A. single seed-soaking method is promptly sowed without cleaning directly after 12 hours with the seed soaking of mass concentration 0.1%～0.3% hydrogen peroxide;
B. mix seed-soaking method, promptly use quality 0.1%～0.3% hydrogen peroxide, 100～300g/L gibberellin, 0.1%～0.3%KNO
3With 0.01～0.1%KMnO
4The mixed liquor seed soaking was sowed without cleaning directly after 12 hours.
Above-mentioned two kinds of methods are all carried out under illumination condition, and light application time is 8-17.5 hour, and intensity of illumination is 350～450umol m
-2s
-1, temperature is between 25～30 ℃.
3) sowing and breeding: in temperature 25-30 ℃, air humidity: under 50%～80% condition, black nightshade seed is seeded in the soil thickness of earth covering 1-2cm on the seed; Soil moisture content is 60～80% of a maxmun field capacity, and controll day, light application time was 8～17.5 hours, can emerge by nature in 8-10 days.
Described soil: pH value 6.5～7.5, the content of organic matter 14.3～20.5g/kg, full N content 0.5～1.1g/kg, full P content 0.5～0.9g/kg, effective P content 8-15mg/kg, quick-acting K content 70～95mg/kg.
The orthogonal experiment (factor, horizontal correspondence table see Table 1,2) of 4 kinds of single seed soaking of reagent and 4 factors, 3 levels is adopted in this experiment.Before on-test, black nightshade seed is all used mass concentration 1%NaClO solution disinfection 10 minutes, and distilled water flushing is clean and shrivelled kernel floated remove.The seed that disinfects under 30 ℃ of constant temperatures, was soaked seed 12 hours, and with the distilled water seed soaking and seed soaking compare.
Running water seed soaking test is divided into to illumination condition and unglazed according to condition is arranged.It is two kinds of processing methods that the seed-soaking liquid test is divided into, the one, and single seed-soaking liquid is handled, and promptly respectively with potassium nitrate, potassium permanganate, hydrogen peroxide, gibberellin seed soaking, each agent administration level sees Table 1.The 2nd, mix seed-soaking liquid and handle, mixed liquor adopts the orthogonal design of 4 factors, 3 levels, and each is handled horizontal combination and sees Table 4.Each seed-soaking liquid is handled and is divided into seed soaking back cleaning seed again and does not clean two kinds of processing of seed.
In each is handled, choose 50 evenly distributed in the 9cm specification culture dish that is lined with individual layer filter paper, each is handled and repeats 3 times, cultivates in constant incubator and in the greenhouse by solar heat.Wherein, constant incubator is the without a licence optical condition, and culturing room is for there being illumination condition, and light application time is 8～17.5 hours, and intensity of illumination is 350～450umol m
-2s
-1, temperature all is controlled between 25～30 ℃.
The single seed-soaking liquid processing horizontal of table 1. table
Table 2. black nightshade seed germination test is respectively handled the table of comparisons
1.3. result and analysis
In general, crops require the germination rate of crop seed will reach more than 80% at least on producing.A kind of farmland weed plant of black nightshade, grain weight is huge, does not meet the germination standard of production requirement at present.Therefore, this test is germinateed with above-mentioned crops and is required a evaluation criterion as black nightshade seed germination rate height, promptly reaches more than 80% to be germination rate preferably.
1.3.1 the effect of illumination condition
As can be seen from Table 3, illumination condition has material impact to the germination of black nightshade seed, and seed germination rate is significantly higher than unglazed according to condition under the illumination condition.Occur to germinate since the 3rd day, unglazed according under the condition, percentage of seedgermination only 0.7～2.7%, and when illumination condition was arranged, germination rate then can during to off-test, have the germination rate of illumination condition to be about unglazed 5 times of the conditions of shining up to 2.7～13.2%.Therefore, illumination condition is one of essential condition of black nightshade germination.
The germination rate (%) of black nightshade under the table 3 running water seed soaking condition
1.3.2 the influence that single seed-soaking liquid germinates to black nightshade seed
From table 4-5 as can be seen, the processing percentage of seedgermination of soaking seed through potassium per nitrate, potassium permanganate, hydrogen peroxide, 4 kinds of reagent of gibberellin all is better than the contrast of soaking seed with running water, and when germination test finished, the germination rate comparison was up to 6.4 times (table 5) according to raising.But the percentage of seedgermination that cleans again after the seed soaking is starkly lower than the processing of directly germinateing without cleaning in the seed soaking back, and the two differs nearly 2 times, this explanation, and the treatment effect that directly germinates without cleaning in the seed soaking back is better.
In the processing that potassium nitrate, potassium permanganate, hydrogen peroxide, 4 kinds of seed-soaking liquids of gibberellin directly germinate after seed soaking (table 5), through the seed of hydrogen peroxide (0.1%～0.3%) seed soaking during at the 5th day germination rate surpass 80%, be respectively 84.7%, 88% and 92.7%, the time when finishing than test shortened near half.Though the germination rate through potassium nitrate and hydrogen peroxide seed soaking processing black nightshade seed also can reach more than 80% in off-test, it is long through the germinating time of hydrogen peroxide (0.1%～0.3%) seed soaking processing that germination rate surpasses 80% o'clock required time ratio.And the percentage of seedgermination of handling through potassium permanganate obviously is inferior to above-mentioned 3 kinds of seed soaking agents.In addition, the contrast seedling is tiny, little Huang, and a little less than the growing way, and all relatively more healthy and stronger through the seedling of seed soaking processing, its leaf look and growing way all are better than the seedling of handling without seed-soaking liquid.
To sum up, think best with the treatment effect of hydrogen peroxide (0.1%～0.3%) in 4 kinds of seed-soaking liquids.Germination rate during its off-test reaches 94～95%, and it is 5 days that germination rate surpassed for 80% used time, and germination rate is 84.7%～92.7%.
1.3.3 mix the influence that seed-soaking liquid germinates to black nightshade seed
From table 6-7 as can be seen, the processing percentage of seedgermination of soaking seed through 9 kinds of mixed liquors all is better than the contrast of soaking seed with running water, and when germination test finished, the germination rate comparison was up to 6.6 times (table 7) according to raising.。But the percentage of seedgermination that cleans again after the seed soaking is starkly lower than the processing of directly germinateing without cleaning in the seed soaking back, and the two differs nearly 1 times, this explanation, and the treatment effect that directly germinates without cleaning in the seed soaking back is better.
Mix in the processing that seed-soaking liquid directly germinates after seed soaking (table 7), the seed of handling through mixed liquor 3,7 and 9 seed soaking during at the 5th day germination rate surpass 80%, be respectively 85.3%, 86.3% and 96.7%, the time during than the test end shortened near half.Though when off-test, the germination rate of all 9 processing has all surpassed 80%, it is the shortest that the black nightshade seed germination rates that mixed liquor 3,7 and 9 is handled surpass 80% o'clock required time, and the germination rate during off-test is especially up to 97.3%～100%.In addition, the contrast seedling is tiny, little Huang, and a little less than the growing way, and all relatively more healthy and stronger through the seedling of seed soaking processing, its leaf look and growing way all are better than the seedling of handling without seed-soaking liquid.
To sum up, thinking 9 kinds, to mix in the seed-soaking liquids treatment effect with mixed liquor 3,7 and 9 good.Germination rate during its off-test reaches 97.3%～100%, and it is 5 days that germination rate surpassed for 80% used time, and germination rate is 85.3%～96.7%.
Comprehensive single seed-soaking liquid of contrast and the stratification effect (table 4～7) of mixing seed-soaking liquid, mixed liquor 3,7 and 9 treatment effect is better than the treatment effect of hydrogen peroxide, the mechanism of its stratification and growth may be that mixed liquor has slackened the inhibiting substances of seed sprouting, promotes the cell interior metabolic activity.But because just adopted a kind of seed soaking agent, the use cost of hydrogen peroxide is lower.Therefore, consider from the comprehensive angle of germination technology and production cost, think that single seed-soaking liquid hydrogen peroxide (0.1%～0.3%) and mixing seed-soaking liquid 3,7 and 9 all are seed-soaking liquids preferably, concrete application is any, and it is fixed to come according to the concrete condition of specification requirement and cost requirement.
The black nightshade seed germination rate (%) of cleaning treatment again after the single seed-soaking liquid of table 4 is handled
3 days 4 days 5 days 6 days 7 days 8 days 9 days 10 days
Do not soak seed 1.3 1.7 3.7 5.0 5.1 8.3 8.3 8.3
Running water soaks seed 2.7 4.0 7.3 9.4 12.3 13.0 13.0 13.2
0.1% potassium nitrate soaks seed 1.3 10.0 24.0 24.7 28.0 31.3 32.0 35.3
0.2% potassium nitrate soaks seed 0.0 11.3 20.0 25.3 29.3 30.0 31.3 33.3
0.3% potassium nitrate soaks seed 2.0 12.7 21.3 26.0 30.0 33.3 36.0 36.7
0.01% potassium permanganate soaks seed 1.3 13.3 19.3 22.7 26.7 27.3 28.0 28.7
0.05% potassium permanganate soaks seed 2.0 10.7 27.3 30.7 36.0 40.0 46.0 46.7
0.1% potassium permanganate soaks seed 2.0 10.7 24.0 34.7 37.3 44.0 47.3 52.7
0.1% hydrogen peroxide soaks seed 0.0 8.0 13.3 16.0 16.7 18.7 18.7 21.3
0.2% hydrogen peroxide soaks seed 2.7 11.3 20.7 22.0 24.7 28.0 31.3 32.7
0.3% hydrogen peroxide soaks seed 1.3 8.7 19.3 25.3 28.0 29.3 30.0 30.7
100ppm gibberellin soaks seed 0.7 8.7 18.0 20.7 22.0 22.0 22.0 22.0
200ppm gibberellin soaks seed 0.7 5.3 22.0 29.3 30.7 34.0 34.7 36.0
300ppm gibberellin soaks seed 0.7 10.0 22.7 26.7 33.3 44.0 38.0 39.3
The black nightshade seed germination rate (%) of cleaning treatment not after the single seed-soaking liquid of table 5 is handled
Table 6 mixes the black nightshade seed germination rate (%) that cleans again after seed-soaking liquid is handled
Handled 3 days 4 days 5 days 6 days 7 days 8 days 9 days 10 days
Do not soak seed 1.3 1.7 3.7 5.0 5.1 8.3 8.3 8.3
Running water soaks seed 2.7 4.0 7.3 9.4 12.3 13.0 13.0 13.2
Mixed liquor 1 6.0 20.7 26.0 36.0 39.3 41.3 42.0 43.3
Mixed liquor 2 4.0 8.72 4.7 30.7 41.3 41.3 41.3 41.3
Mixed liquor 3 6.7 19.3 31.3 38.0 40.0 44.7 48.7 45.3
Mixed liquor 4 6.0 17.3 26.7 30.0 44.7 45.3 46.0 46.0
Mixed liquor 5 7.3 26.7 34.7 37.3 41.3 44.7 45.3 44.7
Mixed liquor 6 5.3 22.7 31.3 40.7 44.7 47.3 48.0 49.3
Mixed liquor 7 6.0 12.7 22.0 33.3 41.3 41.3 42.7 42.7
Mixed liquor 8 3.3 16.0 28.0 44.7 51.3 52.7 52.7 54.0
11.
Mixed liquor 93 42.7 63.3 72.0 78.0 82.7 82.7 82.7
The black nightshade seed germination rate (%) that does not clean after the seed soaking of table 7 mixed liquor is handled
Handled 3 days 4 days 5 days 6 days 7 days 8 days 9 days 10 days
Do not soak seed 1.3 1.7 3.7 5.0 5.1 8.3 8.3 8.3
Running water soaks seed 2.7 4.0 7.3 9.4 12.3 13.0 13.0 13.2
Mixed liquor 1 8.0 10.0 28.7 46.0 57.3 68.0 78.7 85.3
Mixed liquor 2 13.3 19.3 44.7 62.7 68.0 74.0 84.0 88.7
Mixed liquor 3 14.7 18.7 85.3 86.0 88.7 93.3 96.0 97.3
Mixed liquor 4 7.3 9.3 28.0 41.3 50.7 68.0 71.3 85.3
Mixed liquor 5 8.7 10.7 40.0 56.7 63.3 71.3 82.0 86.7
Mixed liquor 6 8.7 10.7 27.3 51.3 57.3 65.3 80.0 82.0
Mixed liquor 7 16.0 22.0 86.3 87.3 89.3 92.7 98.7 98.7
Mixed liquor 8 6.7 10.7 38.7 55.3 63.3 82.0 98.7 98.7
Mixed liquor 9 22.7 42.0 96.7 100.0 100.0 100.0 100.0 100.0
The field trial that embodiment 2 black nightshade seeds are emerged
2.1 materials and methods
Black nightshade seed is collected in ecological experiment station, Chinese Academy of Sciences Shenyang.Gather ripe Solanum nigrum fruit, seed is cleaned with running water and natural air drying after standby.Chemical reagent potassium nitrate, potassium permanganate, hydrogen peroxide, gibberellin are common agents on the market.
Black nightshade seed is all used mass concentration 1%NaClO solution disinfection 10 minutes, and distilled water flushing is clean and shrivelled kernel floated remove.The seed that disinfects under 30 ℃ of constant temperatures, is used 0.1%, 0.2% He respectively
0.3% hydrogen peroxide, mixed liquor 3 (100g/L gibberellin+0.3%H
2O
2+ 0.3%KNO
3+ 0.1%KMnO
4), mixed liquor 7 (300g/L gibberellin+0.1%H
2O
2+ 0.3%KNO
3+ 0.1%KMnO
4) and mixed liquor 9 (300g/L gibberellin+0.3%H
2O
2+ 0.2%KNO
3+ 0.01%KMnO
4) soaked seed 12 hours, and compare with the processing of not seed soaking and running water seed soaking, standby behind the natural air drying after seed is pulled out.
The field emergence place is ecological experiment station, Chinese Academy of Sciences Shenyang.This geographical position, station be 123 ° 41 of east longitude ', 41 ° 31 of north latitude ', the central area that is located in south, loose distant Plain belongs to temperate zone half moistening continental climate, 5～9 ℃ of mean temperatures, greater than 3100～3400 ℃ of 10 ℃ year active accumulated temperatures, year total 129.0～135.0 kilocalories/cm of spoke amount
2, 127～164 days frost-free seasons, annual precipitation 650～700mm.Soil types is a meadow burozem.The soil basic physical and chemical is a pH value 6.65, organic 16.75g/kg, full N 0.74g/kg, full P 0.69g/kg, effective P 10.32mg/kg, quick-acting K 89.06mg/kg.
At the beginning of 7 months, when soil moisture content be maxmun field capacity 70% the time, will broadcast respectively into big Tanaka through the seed of above-mentioned processing, seed earthing 1cm, seed sprouting is treated in operation such as do not water afterwards under field condition, calculate emergence rate then, each is handled and repeats 3 times.The duration of test temperature is 25～30 ℃, and intensity of illumination is 350～450umol m
-2s
-1, light application time 8～17.5 hours.
2.2 result and analysis
The land for growing field crops result of emerging shows (table 8), when stopping to emerging, the black nightshade seed seedling-growing time of handling through seed-soaking liquid is 8 to 10 days, and emergence rate is 81.6～96.1%, and the black nightshade seed seedling-growing time of handling without seed-soaking liquid is 15 to 16 days, and emergence rate is 69.2% and 72.1%.After seed-soaking liquid is handled, seedling-growing time shortened near half, emergence rate improved handle the black nightshade seedling through seed-soaking liquid height of seedling a little more than the seedling of handling without seed-soaking liquid, and it is relatively more healthy and stronger to handle the black nightshade seedling through seed-soaking liquid, well developed root system, growing way is also stronger.It promotes the mechanism of growth may be the raising to the resistance of seedling of the nitrogen nutrition effect of the potassium nitrate in the mixed liquor and oxidant.
This shows, adopt above-mentioned seed-soaking liquid, both can promote the quick germination of black nightshade seed, also can improve the emergence rate and the seedling-growing time of seed, make seedling relatively more healthy and stronger simultaneously again.
The black nightshade seed test of emerging under table 8 field condition
| Seed treatment | Emergence rate (%) | Seedling-growing time (my god) | Height of seedling (cm) | The growth of seedling situation |
| Not seed soaking | ??69.2 | ??16 | ??0.65 | More tiny, root system is general, a little less than the growing way |
| The running water seed soaking | ??72.4 | ??15 | ??0.71 | More tiny, root system is general, a little less than the growing way |
| The seed soaking of 0.1% hydrogen peroxide | ??81.6 | ??10 | ??0.74 | Relatively more healthy and stronger, well developed root system, growing way is strong |
| The seed soaking of 0.2% hydrogen peroxide | ??82.5 | ??9 | ??0.82 | Relatively more healthy and stronger, well developed root system, growing way is strong |
| The seed soaking of 0.3% hydrogen peroxide | ??84.8 | ??8 | ??0.84 | Relatively more healthy and stronger, well developed root system, growing way is strong |
| Mixed liquor 3 | ??90.2 | ??9 | ??0.85 | Relatively more healthy and stronger, well developed root system, growing way is strong |
| Mixed liquor 7 | ??93.0 | ??9 | ??0.86 | Relatively more healthy and stronger, well developed root system, growing way is strong |
| Mixed liquor 9 | ??96.1 | ??8 | ??0.86 | Relatively more healthy and stronger, well developed root system, growing way is strong |
Claims (5)
1. repair plant black nightshade seed-soaking solution, it is characterized in that: the seed-soaking liquid that with water is solvent consists of,
A. single seed-soaking liquid, mass concentration 0.1%～0.3% hydrogen peroxide;
Or B. mixing seed-soaking liquid, mass concentration 0.1%～0.3% hydrogen peroxide, 100～300g/L gibberellin, mass concentration 0.1%～0.3%KNO
3With mass concentration 0.01～0.1%KMnO
4
2. the application of the described seed-soaking liquid of claim 1 in the repair plant black nightshade fast breeding is characterized in that:
1) seed soaking: under temperature 25-30 ℃ condition, black nightshade seed is soaked in (1). single seed-soaking liquid, quality 0.1%～0.3% hydrogen peroxide; (2). mix seed-soaking liquid, 0.1%～0.3% hydrogen peroxide, 100～300g/L gibberellin, 0.1%～0.3%KNO
3With 0.01～0.1%KMnO
4
Pull out after 8-16 hour and dry;
2) sowing and breeding: in temperature 25-30 ℃, air humidity: under 50%～80% condition, black nightshade seed is seeded in the soil thickness of earth covering 1-2cm on the seed; Soil moisture content is 60～80% of a maxmun field capacity, and controll day, light application time was 8～17.5 hours, can emerge by nature in 8-10 days.
3. according to the described application of claim 2, it is characterized in that: described soil: pH value 6.5～7.5, the content of organic matter 14.3～20.5g/kg, full N content 0.5～1.1g/kg, full P content 0.5～0.9g/kg, effective P content 8-15mg/kg, quick-acting K content 70～95mg/kg.
4. according to the described application of claim 2, it is characterized in that:
Black nightshade seed is all used mass concentration 1-2%NaClO solution disinfection 5-15 minute before seed soaking, distilled water flushing is clean and shrivelled kernel floated remove, and is standby behind the natural air drying.
5. according to the described application of claim 2, it is characterized in that: described seed soaking process is all carried out under illumination condition, and day illumination time is 8-17.5 hour, and intensity of illumination is 350～450umol m
-2s
-1, temperature is between 25～30 ℃.
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| CN 200810013230 Pending CN101675740A (en) | 2008-09-17 | 2008-09-17 | Repair plant black nightshade seed-soaking solution and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817708A (en) * | 2010-04-29 | 2010-09-01 | 司文会 | Plant source seed soaking agent and preparation method thereof |
| CN103190223A (en) * | 2013-02-27 | 2013-07-10 | 中国科学院东北地理与农业生态研究所 | Method for improving seed germination of solanum nigrum |
| CN104430511A (en) * | 2014-12-04 | 2015-03-25 | 浙江大学 | Seed priming solution and method |
| CN106211858A (en) * | 2016-07-29 | 2016-12-14 | 柳州创宇科技有限公司 | A kind of processing method improving black nightshade seed germination rate |
| CN107493732A (en) * | 2017-10-17 | 2017-12-22 | 武汉市农业科学院 | A kind of rice paddy seed processing method for being used to improve rice germination percentage |
| CN107853096A (en) * | 2017-11-02 | 2018-03-30 | 成都金川田农机制造有限公司 | The method for planting of black nightshade |
-
2008
- 2008-09-17 CN CN 200810013230 patent/CN101675740A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817708A (en) * | 2010-04-29 | 2010-09-01 | 司文会 | Plant source seed soaking agent and preparation method thereof |
| CN101817708B (en) * | 2010-04-29 | 2012-11-21 | 司文会 | Plant source seed soaking agent and preparation method thereof |
| CN103190223A (en) * | 2013-02-27 | 2013-07-10 | 中国科学院东北地理与农业生态研究所 | Method for improving seed germination of solanum nigrum |
| CN104430511A (en) * | 2014-12-04 | 2015-03-25 | 浙江大学 | Seed priming solution and method |
| CN104430511B (en) * | 2014-12-04 | 2018-06-01 | 浙江大学 | Seed priming liquid and initiation method |
| CN106211858A (en) * | 2016-07-29 | 2016-12-14 | 柳州创宇科技有限公司 | A kind of processing method improving black nightshade seed germination rate |
| CN107493732A (en) * | 2017-10-17 | 2017-12-22 | 武汉市农业科学院 | A kind of rice paddy seed processing method for being used to improve rice germination percentage |
| CN107853096A (en) * | 2017-11-02 | 2018-03-30 | 成都金川田农机制造有限公司 | The method for planting of black nightshade |
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