CN101672852A - Blood type test card and production method thereof - Google Patents
Blood type test card and production method thereof Download PDFInfo
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- CN101672852A CN101672852A CN200910091673A CN200910091673A CN101672852A CN 101672852 A CN101672852 A CN 101672852A CN 200910091673 A CN200910091673 A CN 200910091673A CN 200910091673 A CN200910091673 A CN 200910091673A CN 101672852 A CN101672852 A CN 101672852A
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Abstract
The invention relates to a blood type test card and a production method thereof, belonging to the technical field of blood type test. The blood type test card consists of a carrier and an antibody layer fixed on the carrier, wherein the antibody layer contains antibody print with at least one letter shape. The blood type test card can be widely applied to a main blood type system of ABO, Rh, P, MNSs, Duffy and the like with clinical significance, is easy to popularize and use as well as simple and convenient for operation, leads the blood type result to be directly read and also can avoid theerror caused by human factor.
Description
Technical field
The present invention relates to a kind of blood type test card and production method thereof, belong to blood group detection technique field.
Background technology
Blood group detects mainly according to the blood group positive definite form, and blood group positive definite form at present is according to the agglutinating reaction of blood group antibody with corresponding red cell antigens always.With the abo blood group is example: the red blood cells of type A surface contains A antigen, and blood serum contains B antibody; The Type B erythrocyte surface contains B antigen, contains A antibody in the blood serum; AB type erythrocyte surface contains AB antigen, does not contain AB antibody in the blood serum; O type blood surface does not contain AB antigen, contains AB antibody in the blood serum.The agglutinating reaction that this antigen-antibody forms comes sentence read result by the medical professional according to blood grouping reagent.
The blood group positive definite form detects and mainly to comprise for 4 steps: 1, add blood grouping reagent on reaction vessel or flat board, 2, add a small amount of person under inspection's whole blood, 3, with both mixings, 4, sentence read result.Blood group detects and the result judges and must be finished by the medical professional, and (the reagent position is obscured/remembered unclear, sample add wrong position etc.) causes blood group misjudgment as a result to cause serious transfusion reaction even death because multiple human factor.
Testing process need be used antibody-solutions, and the storage temperature of antibody-solutions is generally 4 degrees centigrade, to prevent inefficacy.There is big difficulty in liquid antibody reagent aspect transportation and preservation, especially (for example open-air) usually can can't obtain testing result accurately because antibody lost efficacy when using in the environment of not stablizing refrigerating equipment.
For simplifying the blood group testing process, detection mode easily is provided, people have carried out following trial:
Chinese patent application 200320121646.2 (publication number is 2669196) discloses a kind of detection abo blood group test card.The blood group card is made of the antibody membrane of band groove flat carrier and groove inner drying, and antibody membrane is respectively anti-A, anti-B and panagglutinin.The transparent non-setting adhesive film is used to cover blood group card mark district and the detection zone that detection finishes, and is used to paste archives.Test card, transparent non-setting adhesive film and the drying agent aluminium foil bag of packing into becomes a kit.This test card room temperature preservation, the result is accurate, can effectively control the effect phase, has made things convenient for layman's use, and the result preserves and files conveniently, and single part of mensuration can be carried.Be mainly used in hospital, army, department of the public security organs abo blood group of going out and detect, the individual also can carry out the blood group self check.
The blood group identification card of Chinese patent application 03215617.0 (publication number is 2605574), a kind of easy blood group identification card is disclosed, the integrated circuit board shape, its integrated circuit board is provided with the evaluation pit, pit is provided with identification marking, preferably will identify three of pit designs, and be respectively anti-A and identify that pit, anti-B identify that pit and RH identify pit; Use the papery integrated circuit board, can have to set up defences on the surface of identifying pit at integrated circuit board and ooze rete.The utility model is simple in structure, use easy to operate, the result quick and precisely, cost is low again, is easy to penetration and promotion and uses.
Chinese patent ZL 200620112928.X (publication number is 2901313) discloses a kind of abo blood group reverse type kit, comprise three air-tight bottles, the A type, Type B and O type red blood cell reagent, air-tight bottle is a plastic dropping bottle, by transparent plastic bottle, plastic bottle closure, dropper and sealing gasket are formed, the A type, Type B and O type red blood cell reagent are by the A type, Type B and O type red blood cell and rbc preservative are formed, the A type, Type B and the erythrocytic concentration of O type are 4%, the A type, Type B and O type red blood cell are the red blood cells after filtering by leucocyte filter, and rbc preservative is to use medicine and distilled water formulated according to special ratios.The term of validity of the present utility model was not less than 3 months, the utility model has the advantages that easy to use, the term of validity long, highly sensitive, abo blood group reverse type kit that accuracy is good, can diagnose abo blood group clinically exactly.
Above invention all can only detect abo blood group, and can't thoroughly eliminate because the interpretation mistake that the people causes for maloperation.Therefore, need a kind ofly to reduce significantly that blood group interpretation error rate, operation are simple more at present, the conspicuous blood type test card of result and/or blood type testing methods.
Summary of the invention
The technical problem to be solved in the present invention is: provide a kind of easy to use, the novel blood type test card that reliable results is obvious.
Another technical matters that the present invention will solve is: the production method that above-mentioned blood type test card is provided.
For achieving the above object, the present invention is by the following technical solutions:
A kind of blood type test card is made up of carrier and the antibody layer that is fixed on the carrier, and antibody layer contains the antibody trace of at least one letter shapes.The quantity of antibody trace can also be combined to form detecting unit with the antibody trace of different letters for one or more.For example, form the abo blood group detecting unit with alphabetical A shape antibody trace and the combination of letter b shape antibody trace.
Described antibody trace is detected by blood group and makes with antibody, and antibody wherein is selected from one or more in anti-A antibody, anti-B antibody, anti-Rh blood group associated antibodies, anti-P blood group associated antibodies, anti-MNSs blood group associated antibodies, the anti-Duffy blood group associated antibodies.
The letter shapes of described antibody trace is alphabetical accordingly with the blood group of this antibody trace generation antigen-antibody reaction.For example the anti-A antibody trace is letter " A ", is measured as A type blood; The anti-B antibody trace is letter " B ", is measured as the B blood group; Anti-Rh antibody trace is letter " R ", is measured as the Rh blood group; Anti-P antibody trace is letter " P ", is measured as the P blood group, anti-MNSs antibody trace is letter ' M ', is measured as the MNSs blood group; Anti-Duffy antibody trace is letter " D ", is measured as the Duffy blood group.
In the present invention, the antibody trace letter shapes corresponding to abo blood group is respectively the shape of alphabetical A or B.Just can determine the blood group of its blood according to the red A that demonstrates, B letter, promptly alphabetical A judges that its blood group is the A type; Letter b judges that its blood group is a Type B; Letter AB judges that its blood group is the AB type; Do not show that any its blood group of letter judgement is the O type.
Antibody trace letter shapes corresponding to Rh, P, MNSs, Duffy blood group is respectively their corresponding letters shape.The Rh blood group contains 5 kinds of antigen DCcEe, this letter of R occurs and judges that its blood contains D antigen, is judged as the Rh positive, letter do not occur and judges that its blood is the Rh feminine gender.Blood groups such as P, MNSs, Duffy are judged identical with the Rh blood group.
Antibody in the described antibody trace is the golden labeling antibody of polyclonal antibody, monoclonal antibody or colloid gold label.Wherein the high specificity of monoclonal antibody and golden labeling antibody can strengthen the sensitivity of reflection.Polyclonal antibody and monoclonal antibody all can be bought by commercial sources and obtain, and also can adopt known polyclonal antibody or MONOCLONAL ANTIBODIES SPECIFIC FOR method to make.
Described carrier is selected from a kind of in cardboard, glass sheet, plastic plate, tunica fibrosa or the resin plate, also can be other acceptable medical materials in check field.Described cardboard is a kind of certain thickness cardboard that has that can bear the inventive method processing.Described glass sheet is generally to sell microslide in the market.Described plastic plate is the polymer sheet of selling in the market, for example polymer sheets such as polystyrene, tygon, Polyvinylchloride, polypropylene, polycarbonate.Described tunica fibrosa is the tunica fibrosa of selling in the market, for example as fibroin protein film, polylactic acid membrane glass fibre membrane, cellulose acetate membrane or nitrocellulose filter etc.
Described resin plate is the resin plate of selling in the market, for example as PVC, ABS, epoxy resin or phenolics plate etc.
The local surfaces of described carrier one side surface or a side has radian, for example is convex-surface type structure or concave structure, and is and for example fluted in the outer periphery of convex-surface type structure.Have in the structure of convex-surface type, antibody layer is positioned at the convex surface place, and the blood with antibody response does not leave the antibody layer surface automatically, can reduce the interference that the result judges.
Described blood type test card is provided with wrappage outward, is selected from plastic sheeting, aluminium foil or the paper one or more.Described plastic sheeting, aluminium foil or paper all are normally used wrappage in drug packaging.Can use the homogenous material packing, for example only be to use plastic film packaging or use In Aluminium Foil Packing, also can use multiple material packing, for example use In Aluminium Foil Packing earlier, again in its outside wrapper.
A kind of production method of blood type test card, use antibody detecting with the surface spraying of antibody fixing agent pretreated cleaning carrier or printing and dyeing blood group, make the antibody layer of the antibody trace with letter shapes, the use damping fluid is removed not and carrier-bound unnecessary antibody, drying, packing.
The letter shapes of described antibody trace is alphabetical accordingly with the blood group of this antibody trace generation antigen-antibody reaction.
The method of described cleaning carrier includes but not limited to following method: when using paper carrier, can use the suction cleaner dust out earlier, spraying fungicide, sanitizer etc. clean then; When using glass sheet, plastic plate or resin plate, can soak 12-24h with washing lotion earlier, with the tap water flushing, clean with distilled water more then; Filter can at room temperature be carried out air dry after doing, and also can carry out drying at drying box again after drying with silk.When using tunica fibrosa, can spray the detersive of on market, selling at present, then airing or carry out drying at drying box.
Described pretreated cleaning carrier is meant with the antibody fixing agent and has carried out pretreated carrier, but its surface binding antibody.
Because solid phase carrier itself does not generally have directly and the ability of antibody and the reaction of other activated proteins, therefore need to introduce reactive group at surface of solid phase carriers in advance, utilize amino, the carboxyl on reactive group and the antibody then or other groups react and it is fixed on the described carrier.For this reason, the carrier of cleaning need be handled with the antibody fixing agent.
In the present invention, the antibody fixing agent should be appreciated that it is to introduce reactive group toward surface of solid phase carriers, and the amino of these reactive groups and antibody, carboxyl isoreactivity group reacts and it is fixed on any material on the described carrier.Fixing agent commonly used can be inorganic fixing agent or organic fixing agent.
Described inorganic fixing agent is methyl alcohol, ethanol, acetone, formaldehyde, glutaraldehyde, silicon dioxide and osmic acid etc. for example, and they all are product solds in the market.For example silicon dioxide is sprayed on the carrier, by surface hydroxylation, amino silaneization and terephthalaldehyde assembling, in the silica surface active aldehyde groups of deriving, utilize the amino of antibody and aldehyde radical generation reductive amination process that antibody is fixed on the silica surface.
Described organic fixing agent for example is APES (3-aminopropyl triethoxysilane), Polv-L-Lysine (poly-D-lysine) or chromium gelatin solution etc., and they all are product solds in the market.When for example using fibroin protein film or polylactic acid membrane, the organic fixing agent that use contains amino, carboxyl or other groups is handled fibroin protein film or polylactic acid membrane, the amino of antibody and aldehyde radical and described these groups is reacted and antibody is fixed on these films.
The use amount of described fixing agent is not particularly limited, and the use amount of described fixing agent can not be very little, if use amount very little, described carrier and antibodies are not firm, take off dew easily, thereby influence testing result.If the fixing agent use amount is too many, can cause the waste of described fixing agent.Generally speaking, the using method of described fixing agent is: at the carrier of cleaning and the surperficial spraying fixation agent of sessile antibody layer, maybe will clean carrier and place fixative solution to make fixing agent on the surface coverage, and take out; Dry getting final product.
Described fixing agent is selected from a kind of in methyl alcohol, ethanol, acetone, formaldehyde, glutaraldehyde, silicon dioxide, osmic acid, 3-aminopropyl triethoxysilane, poly-D-lysine or the chromium gelatin solution.The method of antibody and carrier secure bond is a prior art, and those of ordinary skills can implement easily according to the guidance of the operation instructions of textbook, document or fixing agent.
Described use damping fluid is removed not and to be meant to detect with some reactive groups in antibody and the carrier surface fixing agent with carrier-bound unnecessary antibody and to react and after being fixed on the carrier, place a period of time (0.5-24 hour), treat to remove not and carrier-bound unnecessary antibody with damping fluid again after immobilization process finishes, obtain described blood type test card.
Described damping fluid is a kind of in phosphate buffer, carbonate buffer solution or the glycerinated damping fluid.Described damping fluid is not for can will removing and don't damage the solution of other binding antibody and blood type test card other parts with this carrier-bound unnecessary antibody.The damping fluid use amount is not particularly limited, more or less can, but be principle not damage other binding antibody and blood type test card other parts.
After using described damping fluid to handle, generally need carry out dried (room temperature or vacuum drying chamber).
Blood type test card using method of the present invention is: add a small amount of person under inspection's whole blood on blood type test card of the present invention, normally 1-2 drips whole blood, shakes up the red letter that the back shows on the blood type test card according to the present invention, just can know the blood group result.The red alphabetical A that shows on the blood type test card for example of the present invention represents that person under inspection's blood group is an A type blood, and the rest may be inferred for other.
Blood type test card of the present invention can be widely used in the main blood group system that ABO, Rh, P, MNSs, Duffy etc. have clinical meaning.Blood type test card of the present invention can be applicable to each Blood Center, hospitals at different levels Blood Transfusion Dept., clinical laboratory, accident, battlefield medical treatment are standby, the medical aid station, backcountry, non-medical professional's family expenses, both can be designed to identify in batches at Blood Center and hospital, again can at family expenses be designed to single or ten people with interior a small amount of detection type card.
Advantage of the present invention is: the present invention is fixed in antibody on the carrier, needn't re-use the antibody-solutions that refrigerates in the prior art, has thoroughly solved a transportation and a refrigeration difficult problem of carrying antibody-solutions, and has greatly simplified detecting operation.The present invention is simple to operate, only need droplets of whole blood is added in reaction place, shake a little, can be according to the direct interpretation blood group result of the letter that manifests behind the erythrocyte aggregation, only need just direct knowledge of result of 2 steps, greatly shortened detection time, the blood group misjudgment that can also avoid human factor to cause, for example contingent blood grouping reagent adds mistake, adds mistakes such as anti-, that blood group result misreads in traditional blood group detecting operation.The present invention is with a wide range of applications as detecting blood group in Blood Center, hospital, accident, battlefield medical treatment, backcountry rescue or family.
The invention will be further described below in conjunction with the drawings and specific embodiments; it is not qualification to invention; according to prior art well known in the art; embodiments of the present invention are not limited to this; therefore all this areas of being made according to this paper disclosure be equal to replacement, all belong to protection scope of the present invention.
Description of drawings
Fig. 1 is first kind of structural representation of the present invention, and carrier is a planar structure.
Fig. 2 is the longitudinal diagram of first kind of structure of the present invention.
Fig. 3 is second kind of structural representation of the present invention, and carrier has concave surface.
Fig. 4 is the longitudinal diagram of second kind of structure of the present invention.
Fig. 5 is the third structural representation of the present invention, and carrier has convex surface and groove.
Fig. 6 is the longitudinal diagram of the third structure of the present invention.
Fig. 7 is the synoptic diagram of person-time blood type test card.
Embodiment
The abo blood group test card is made up of with antibody layer 2 plane slide carrier 1 (medical microslide) and the detection that is fixed on the slide carrier 1, and antibody layer is made up of two antibody traces.
The letter shapes of antibody trace is respectively alphabetical A and letter b, and the antibody trace of alphabetical A shape is an anti-A antibody, and the antibody trace of letter b shape is an anti-B antibody.
The abo blood group test card is made up of with antibody layer 2 cardboard carrier 1 and the detection that is fixed on the cardboard carrier 1, and antibody layer is made up of two antibody traces.
The letter shapes of antibody trace is respectively alphabetical A and letter b, and the antibody trace of alphabetical A shape is an anti-A antibody, and the antibody trace of letter b shape is an anti-B antibody.The surveyed area of cardboard carrier 1 has concave structure 3, and the antibody trace is positioned at concave structure 3 places.
The abo blood group test card is made up of with antibody layer 2 cardboard carrier 1 and the detection that is fixed on the cardboard carrier 1, and antibody layer is made up of two antibody traces.
The letter shapes of antibody trace is respectively alphabetical A and letter b, and the antibody trace of alphabetical A shape is an anti-A antibody, and the antibody trace of letter b shape is an anti-B antibody.The surveyed area of cardboard carrier 1 has convex configuration 3, and antibody layer is positioned at convex configuration 3 places, and being peripherally installed with of convex configuration 3 is fluted 4, is used to collect unreacted blood.
The abo blood group test card is made up of cardboard carrier 1 and the antibody layer 2 that is fixed on the cardboard carrier 1, and antibody layer 2 is made up of several detecting units, and each detecting unit is made up of the antibody trace of two single-letter shapes.
The letter shapes of the antibody trace in each detecting unit is respectively alphabetical A and letter b, and the antibody trace of alphabetical A shape is an anti-A antibody, and the antibody trace of letter b shape is an anti-B antibody.
The surveyed area of cardboard carrier 1 has convex configuration 3, and antibody layer is positioned at convex configuration 3 places, and being peripherally installed with of convex configuration 3 is fluted 4, is used to collect unreacted blood.
This abo blood group test card can realize that simultaneously many people being carried out blood group detects, and the edge blank space can be done the usefulness of detection record.
Embodiment 5 adopts the production method of medical glass slide glass as the blood type test card of carrier
A, select for use medical glass slide glass (slide) as carrier, its washing methods is to soak diel with 1% HCl solution, scrubs with commercially available washing agent again, washes repeatedly with clear water then, uses distilled water flushing at last 1～2 time.After filter is done, in drying box, under 90 ℃ of temperature, carry out dry 2h again, be cooled to room temperature then.
B, the 3-aminopropyl triethoxysilane of selling with Shandong Province Qufu City China flourish new chemical materials company limited on the cleaning carrier are handled, and take out slide with this reagent after with the slide submergence, and drying is standby;
C, spray the antibody trace of alphabetical A and B shape with corresponding antibodies on treated carrier, the antibody trace of alphabetical A shape adopts the anti-A antibody spraying, and the antibody trace of letter b shape adopts anti-B antibody spraying, spend the night (placing 0.5-24 hour); (antibody is available from Shanghai blood biomedical limited liability company, and commodity are by name: anti-A, anti-B blood grouping reagent)
D, remove not and carrier-bound unnecessary antibody, dry with phosphate buffer.
E, the blood type test card that obtains pack with the aluminium pool, and many person-portions or single part all can.
Embodiment 6: adopt cardboard as carrier production blood type test card of the present invention
The production of blood type test card of the present invention, its production stage is as follows:
A, select for use disposable blood group cardboard as carrier, it is the circular groove of 1.2-2.2cm that cardboard is processed into each detecting unit diameter, and the degree of depth of each circular groove is 0.02-0.2cm, and the centre is circular-arc.
B, on treated fibroin protein film/nitrocellulose membrane with the antibody trace of corresponding antibodies spray printing letter A and B shape, the antibody trace of letter A shape adopts the anti-A antibody spraying, the antibody trace of letter b shape adopts the anti-B antibody spraying, spend the night (placing 0.5-24 hour), remove not and carrier-bound unnecessary antibody with phosphate buffer, dry, make the shape of detecting unit, standby; (antibody is available from Shanghai blood biomedical limited liability company, and commodity are by name: anti-A, anti-B blood grouping reagent)
C, with coated with adhesive in the middle of each detecting unit, the film that scribbles antibody is attached in the detecting unit;
D, obtain blood type test card of the present invention;
E, the blood group that obtains stick into row and pack.
Use the embodiment 1 described blood type test card of the method production of embodiment 5,100 person-times of detections have been carried out, the result shows, the testing result that non-medical professional uses this blood type test card to obtain is identical with the testing result that present medical professional adopts traditional blood type testing methods to obtain, non-medical professional uses this blood type test card detection speed fast again, produces cost in batches and can be lower than existing traditional blood type testing methods.Therefore, be very beneficial for wide popularization and application.
Claims (20)
1. blood type test card is characterized in that: be made up of carrier and the antibody layer that is fixed on the carrier, antibody layer contains the antibody trace of at least one letter shapes.
2. a kind of blood type test card according to claim 1, it is characterized in that: described antibody trace is detected by blood group and makes with antibody, and antibody wherein is selected from one or more in anti-A antibody, anti-B antibody, anti-Rh blood group associated antibodies, anti-P blood group associated antibodies, anti-MNSs blood group associated antibodies, the anti-Duffy blood group associated antibodies.
3. a kind of blood type test card according to claim 1 is characterized in that: the letter shapes of described antibody trace is for alphabetical accordingly with the blood group of this antibody trace generation antigen-antibody reaction.
4. according to any one described a kind of blood type test card in the claim 1 to 3, it is characterized in that: the antibody in the described antibody trace is the golden labeling antibody of polyclonal antibody, monoclonal antibody or colloid gold label.
5. a kind of blood type test card according to claim 1 is characterized in that: described carrier is selected from a kind of in cardboard, glass sheet, plastic plate, tunica fibrosa or the resin plate.
6. a kind of blood type test card according to claim 5 is characterized in that: described plastic plate is selected from a kind of in the polymer sheets such as polystyrene, tygon, Polyvinylchloride, polypropylene or polycarbonate.
7. a kind of blood type test card according to claim 5 is characterized in that: described tunica fibrosa is selected from a kind of in fibroin protein film, polylactic acid membrane glass fibre membrane, cellulose acetate membrane or the nitrocellulose filter.
8. a kind of blood type test card according to claim 5 is characterized in that: described resin plate is selected from a kind of in PVC, ABS, epoxy resin or the phenolics plate.
9. a kind of blood type test card according to claim 1 is characterized in that: the local surfaces of described carrier one side surface or a side has radian.
10. a kind of blood type test card according to claim 9 is characterized in that: the local surfaces of described carrier one side surface or a side has radian, is convex-surface type or concave structure.
11. a kind of blood type test card according to claim 10 is characterized in that: the outer periphery of described convex-surface type structure is fluted.
12. a kind of blood type test card according to claim 1 is characterized in that: described blood type test card is provided with wrappage outward, is selected from plastic sheeting, aluminium foil or the paper one or more.
13. the production method of a blood type test card, it is characterized in that: at surface spraying or printing and dyeing blood group detection antibody with the pretreated cleaning carrier of antibody fixing agent, make the antibody layer of antibody trace with letter shapes, use damping fluid to remove not and carrier-bound unnecessary antibody, drying, packing.
14. the production method of a kind of blood type test card according to claim 13 is characterized in that: the letter shapes of described antibody trace is for alphabetical accordingly with the blood group of this antibody trace generation antigen-antibody reaction.
15. the production method of a kind of blood type test card according to claim 13 is characterized in that: the method for described cleaning carrier is: when using paper carrier, and first dust out, spraying fungicide, sanitizer etc. clean then; When using glass sheet, plastic plate or resin plate, soak 12-24h with washing lotion earlier, water flushing then; Filter is carried out drying after doing; When using tunica fibrosa, spray detersive, carry out drying then.
16. the production method of a kind of blood type test card according to claim 13 is characterized in that: described fixing agent is inorganic fixing agent or organic fixing agent.
17. the production method of a kind of blood type test card according to claim 13 is characterized in that: described inorganic fixing agent is selected from one or more in methyl alcohol, ethanol, acetone, formaldehyde, glutaraldehyde, silicon dioxide or the osmic acid.
18. the production method of a kind of blood type test card according to claim 13 is characterized in that: described organic fixing agent is selected from one or more in 3-aminopropyl triethoxysilane, poly-D-lysine or the chromium gelatin solution.
19. the production method of a kind of blood type test card according to claim 13, it is characterized in that: described use damping fluid is not removed and is meant with carrier-bound unnecessary antibody, detect with some reactive groups in antibody and the carrier surface fixing agent and react and after being fixed on the carrier, place a period of time (0.5-24 hour), treat to remove not and carrier-bound unnecessary antibody with damping fluid again after immobilization process finishes, obtain described blood type test card.
20. the production method of a kind of blood type test card according to claim 13 is characterized in that: described damping fluid is selected from a kind of in phosphate buffer, carbonate buffer solution or the glycerinated damping fluid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910091673A CN101672852A (en) | 2009-08-28 | 2009-08-28 | Blood type test card and production method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN200910091673A CN101672852A (en) | 2009-08-28 | 2009-08-28 | Blood type test card and production method thereof |
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| CN101672852A true CN101672852A (en) | 2010-03-17 |
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| CN200910091673A Pending CN101672852A (en) | 2009-08-28 | 2009-08-28 | Blood type test card and production method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103769378A (en) * | 2012-10-24 | 2014-05-07 | 艾博生物医药(杭州)有限公司 | Method and equipment for cleaning reagent mat |
| CN103884849A (en) * | 2014-04-06 | 2014-06-25 | 邵超鹏 | Display manner of determination results of blood type and blood type antibody and manufacturing method |
| CN104569444A (en) * | 2014-12-22 | 2015-04-29 | 合肥天一生物技术研究所 | Forward and reverse ABO typing and RhD blood type detecting card |
-
2009
- 2009-08-28 CN CN200910091673A patent/CN101672852A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103769378A (en) * | 2012-10-24 | 2014-05-07 | 艾博生物医药(杭州)有限公司 | Method and equipment for cleaning reagent mat |
| CN103769378B (en) * | 2012-10-24 | 2016-02-03 | 艾博生物医药(杭州)有限公司 | A kind of method and apparatus for cleaning reagent pad |
| CN103884849A (en) * | 2014-04-06 | 2014-06-25 | 邵超鹏 | Display manner of determination results of blood type and blood type antibody and manufacturing method |
| CN104569444A (en) * | 2014-12-22 | 2015-04-29 | 合肥天一生物技术研究所 | Forward and reverse ABO typing and RhD blood type detecting card |
| CN104569444B (en) * | 2014-12-22 | 2016-03-30 | 合肥天一生物技术研究所 | A kind of positive reverse type of ABO and RhD blood type test card |
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Application publication date: 20100317 |