CN215575177U - Detection test paper - Google Patents
Detection test paper Download PDFInfo
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- CN215575177U CN215575177U CN202120565475.0U CN202120565475U CN215575177U CN 215575177 U CN215575177 U CN 215575177U CN 202120565475 U CN202120565475 U CN 202120565475U CN 215575177 U CN215575177 U CN 215575177U
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- 238000012360 testing method Methods 0.000 title claims abstract description 70
- 238000001514 detection method Methods 0.000 title claims description 28
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000002250 absorbent Substances 0.000 claims abstract description 28
- 230000002745 absorbent Effects 0.000 claims abstract description 28
- 238000010521 absorption reaction Methods 0.000 claims abstract description 25
- 238000006243 chemical reaction Methods 0.000 claims abstract description 23
- 239000012528 membrane Substances 0.000 claims abstract description 19
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 11
- 238000012545 processing Methods 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims description 15
- 230000004888 barrier function Effects 0.000 claims description 7
- 239000000523 sample Substances 0.000 abstract description 35
- 208000015181 infectious disease Diseases 0.000 abstract description 7
- 239000012488 sample solution Substances 0.000 abstract description 5
- 208000001490 Dengue Diseases 0.000 abstract description 2
- 206010012310 Dengue fever Diseases 0.000 abstract description 2
- 208000025729 dengue disease Diseases 0.000 abstract description 2
- 201000004792 malaria Diseases 0.000 abstract description 2
- 208000035473 Communicable disease Diseases 0.000 abstract 1
- 201000003176 Severe Acute Respiratory Syndrome Diseases 0.000 abstract 1
- 239000000123 paper Substances 0.000 description 34
- 239000000853 adhesive Substances 0.000 description 11
- 229920000728 polyester Polymers 0.000 description 6
- 239000004743 Polypropylene Substances 0.000 description 4
- 239000012491 analyte Substances 0.000 description 4
- 229920001971 elastomer Polymers 0.000 description 4
- 238000012125 lateral flow test Methods 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 229920001155 polypropylene Polymers 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000004800 polyvinyl chloride Substances 0.000 description 3
- 239000000835 fiber Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 239000004745 nonwoven fabric Substances 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 229920006267 polyester film Polymers 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 241001678559 COVID-19 virus Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000002655 kraft paper Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model provides a test paper, which comprises a support plate, wherein a sample processing pad, a reaction membrane and a water absorption pad are sequentially overlapped on the support plate from upstream to downstream, and a hydrophobic element is arranged at the downstream end of the water absorption pad of the test paper. The hydrophobic element acting as a pinching area may prevent the sample solution on the absorbent pad from flowing further to the area where the operator pinches the test strip. The test paper of the utility model can effectively reduce the infection risk of operators when carrying out sample test, in particular to the infection risk when testing samples with infectious diseases, such as HBV, HIV, malaria, dengue fever, SARS, new crown and the like.
Description
Technical Field
The utility model belongs to the technical field of biological detection, and particularly relates to a structure of detection test paper.
Background
Currently, more and more products that can realize on-site sampling and can immediately obtain the detection result are researched and applied to clinics, and the detection is generally called point-of-care testing (POCT). For example, Lateral Flow Test strips (late Flow Test strips) are currently commonly used in point-of-care products.
Lateral flow test strips utilize the principle of chromatography to transfer a sample on the test strip, and as shown in fig. 1, a lateral flow test strip 100 generally comprises a support plate 1 on which a sample pad 2, a labeled conjugate release pad 3, a reaction membrane 4 and a water absorption pad 5 are adhered, which are sequentially stacked one on top of the other from upstream to downstream. The reaction membrane is generally provided with a detection line 41 and a control line 42, the control line may be upstream or downstream of the detection line, or some detection test strips may not be provided with the control line. And the signal of the detection line on the reaction membrane is linearly related to the content of the analyte in the sample to be detected, so that whether the analyte exists in the sample or the concentration of the analyte is judged. The lateral cross flow test paper can be assembled in a test board consisting of an upper cover plate and a lower cover plate, and the water absorption pad is not exposed to the external environment. The test strip 101 may also be in the form of a strip as shown in FIG. 2, with a sticker 6 attached to the sample pad and the labeled conjugate releasing pad of the test strip.
The solution to be tested can be added to the sample pad of the test strip by dropping, or the lateral flow test strip 101 can be inserted into the small tube containing the solution to be tested by dipping the sample pad in the solution to be tested as shown in fig. 3. The solution to be measured on the sample pad flows through the labeled conjugate releasing pad, the reaction membrane in order, and is finally absorbed by the water absorbing pad. When the test strip 101 in the form of a test strip is used, as shown in fig. 4, an operator needs to hold the absorbent pad and take out the test strip from the small tube to observe the test result or discard the test strip. If the solution to be tested contains infectious pathogenic viruses or bacteria, such as HBV, HCV, HIV, malaria, dengue fever, influenza, respiratory syndrome of the middle east, SARS, and the new crown antigen 2019-nCoV, etc., these pathogenic factors (viruses or bacteria, etc.) remain on the absorbent pad. Once the operator is not wearing gloves or otherwise performing undue manipulation, the fingers holding the pad may be exposed to these agents, which presents a significant risk of infection.
SUMMERY OF THE UTILITY MODEL
The problem that an operator is infected by pathogenic factors remained on a water absorption pad of a detection test paper due to contact with the water absorption pad when the conventional detection test paper is used is solved. The utility model provides a novel and safe test paper.
The utility model aims to provide a test paper, which comprises a support plate, a sample processing pad, a reaction membrane and a water absorption pad which are sequentially stacked from the upstream to the downstream on the support plate, wherein a hydrophobic element is arranged at the downstream end of the water absorption pad of the test paper.
Preferably, the hydrophobic element is a front end of the support plate, the material of the front end being a hydrophobic material and not covered by the absorbent pad.
Preferably, the hydrophobic element is a barrier.
Preferably, the blocking block is adhered to a support plate located downstream of the absorbent pad.
Preferably, the hydrophobic element is a handle.
Further, the sample processing pad may include a sample pad and a labeled conjugate release pad which are independently attached to the support plate while being sequentially overlapped with each other from upstream to downstream.
Furthermore, self-adhesive stickers are stuck on the sample processing pad and the water absorption pad.
Preferably, the self-adhesive sticker is provided with a hand-pinching area indicating mark corresponding to the hydrophobic element.
Furthermore, a detection line and a control line are arranged on the reaction membrane.
Preferably, the area of the hydrophobic element is sized to allow the operator's finger to hold the test strip without touching the absorbent pad.
Another object of the present invention is to provide a method for preparing the test strip, which comprises providing a support plate; the detection pad is adhered to the support plate, the detection line with the detection reagent is coated on the detection pad, after the detection pad is dried, the sample processing pad and the water absorption pad are sequentially adhered to the overlapped adhesion of the support plate, and the hydrophobic element is arranged at the downstream of the water absorption pad.
Further, the hydrophobic element is a barrier.
Further, the sample processing pad includes a sample pad and a labeled conjugate releasing pad which are stacked on each other from upstream to downstream.
Furthermore, a layer of self-adhesive sticker is pasted on the upper surfaces of the sample processing pad and the water absorption pad.
The utility model has the beneficial effects that: the hydrophobic element is arranged at the front end of the detection test paper and can be used as a holding area to prevent the sample solution on the absorbent pad from flowing further to the area where the operator holds the detection test paper. When the test paper is used for detection, particularly when a sample with infectious viruses or bacteria is detected, even if an operator does not carry gloves or other improper operation behaviors, the operator cannot or cannot easily contact the pathogenic factors, so that the infection risk is reduced.
Drawings
FIG. 1 is a schematic view of a prior art test strip with a bibulous pad covering the forward-most end of the support plate.
FIG. 2 is a schematic view of a prior art test strip with a sticker.
FIG. 3 is a schematic representation of the insertion of a test strip into a sample vial.
FIG. 4 is a schematic diagram showing the operator manually pinching the upper end of the test strip to remove the test strip from the sample vial.
FIG. 5 is a schematic view of a test strip of the present invention, wherein the front end of the support plate is not covered by the absorbent pad.
FIG. 6 is a schematic view of the test paper with sticker of the present invention.
FIG. 7 is a schematic representation of a test strip of the present invention with a stop downstream from the absorbent pad.
Fig. 8 is a schematic view of a support plate covered with stickers.
Detailed Description
The test strip 103 shown in fig. 5 is a lateral cross-flow test strip, which comprises a support plate 1, a sample pad 2, a labeled conjugate release pad 3, a reaction membrane 4 and a water absorption pad 5, which are sequentially stacked on the support plate 1 from upstream to downstream, wherein a detection line 41 and a comparison line 42 are arranged on the reaction membrane, a hydrophobic element 7 is arranged at the downstream end of the water absorption pad 5, the region of the hydrophobic element can be used as a hand-pinching region, and an operator can pinch the region to take the test strip. In a preferred embodiment, the hydrophobic element is preferably sized to allow the operator's finger to hold the test strip without touching the absorbent pad.
In contrast to fig. 1, the absorbent pad shown in fig. 5, 6, 7 has an upper edge 51 that is not aligned with the upper edge 11 of the support plate, but is at a distance therefrom. In a preferred embodiment, the specific value of the distance is suitable for the operator's finger to hold the test strip without touching the absorbent pad, so as to minimize the probability of touching the absorbent pad by the operator.
In the examples of the test strip 103 shown in fig. 5 and the test strip 104 shown in fig. 6, the downstream end of the absorbent pad, i.e., the front end of the support plate 1, is not covered with the absorbent pad, and the material of the front end is a hydrophobic material as the hydrophobic member according to the present invention. After the solution to be detected on the sample pad sequentially flows through the labeled conjugate release pad and the reaction membrane to reach the water absorption pad, the sample solution on the water absorption pad cannot be further absorbed by the hydrophobic support plate, so the solution to be detected cannot be remained in the hand-pinching area. After the operator pinches the hand pinch area of the test paper, the risk of infection is not worried about.
The test strip 105 shown in FIG. 7 comprises a support plate 1, and a sample pad 2, a labeled conjugate releasing pad 3, a reaction membrane 4, a water absorbing pad 5, and a blocking block 8 as a hydrophobic member are adhered to each other on the support plate 1 in this order from upstream to downstream. The material of the barrier 8 is hydrophobic and sample solution that has flowed onto the absorbent pad is not absorbed by the barrier.
In other embodiments, the test strip is similar to that shown in FIGS. 5, 6 and 7, except that the absorbent pad has an upper edge aligned with an upper edge of the support plate, and a handle is attached to the pinch area of the test strip, the handle being made of a hydrophobic material.
The test paper shown in FIG. 6 is based on FIG. 5, wherein a sticker 6 is adhered to the sample pad and the labeled conjugate releasing pad of the test paper, and a sticker 6 is adhered to the absorbent pad and a part of the support plate. The corresponding part of the test paper shown in FIG. 7 may also be pasted with a sticker.
In a preferred embodiment, as shown in fig. 6, a finger-pinch-area indicator mark is provided on the sticker corresponding to the finger-pinch area for prompting the operator to pinch the finger there, so as to further avoid the contamination of the operator with the residual solution to be tested due to the wrong position. The indicator may be a pattern, such as a pattern 61 of a hand, or a color indication different from the other areas.
In another preferred scheme, in order to prevent the sample solution remaining on the reaction film from contacting an operator or reduce pollution to the external environment, a transparent self-adhesive sticker is pasted on the reaction film.
In some embodiments, the sample pad and the labeled conjugate releasing pad may be formed as a pad, and the same material may be used, for example, the test strip includes a support plate on which a sample treatment pad, a reaction membrane and a water absorbent pad are adhered, which are sequentially stacked one on another from upstream to downstream, a hydrophobic member is disposed between the upper edge 51 of the water absorbent pad 5 and the upper edge 11 of the support plate 1, the labeled conjugate is treated on the sample treatment pad, and the analyte of the sample reacts with the labeled conjugate on the pad after the sample is added to the sample treatment pad.
The material of the support plate is selected from hydrophobic Polyester (PET) rubber plate, polyvinyl chloride (PVC) rubber plate, polypropylene (PP) rubber plate and Polycarbonate (PC) rubber plate, and the hydrophobic materials can also be used as the material of the hydrophobic element.
The material of the sample pad is selected from glass cellulose, polyester film, polyester fiber film, non-woven fabric, and filter paper.
The marker conjugate releasing pad material is selected from glass cellulose, polyester film, polyester fiber film, and non-woven fabric.
The material of the reaction membrane is selected from NC membrane and nitrocellulose membrane.
The absorbent pad is made of cellulose filter paper, absorbent paper, and filter paper.
The material of the self-adhesive sticker is selected from hydrophobic coated paper, transparent polyvinyl chloride (PVC), electrostatic polyvinyl chloride (PVC), Polyester (PET), laser paper, temperature-resistant paper, polypropylene (PP), Polycarbonate (PC), kraft paper, fluorescent paper, gold-plated paper, silver-plated paper, synthetic paper, aluminum foil paper, fragile (anti-counterfeiting) paper, textured paper, cloth label (Thaik/nylon) paper, pearlescent paper, sandwich coated paper and thermal paper.
FIG. 5 illustrates a method of making the test strip. A support plate 10 shown in FIG. 8 is provided, the support plate 1 is pre-attached with a sticker 12 corresponding to the hydrophobic element of the test strip, 13 corresponding to the absorbent pad, 14 corresponding to the reaction membrane, and 15 corresponding to the sample pad and the labeled conjugate release pad. First, the sticker 14 is removed, a reaction film is attached to the support plate from which the sticker 14 is removed, and corresponding detection reagents are treated on the detection line 41 and the control line 42 of the reaction film. After the detection reagent on the reaction film is dried, the self-adhesive sticker 13 is removed, the water absorption pad 5 is attached to the position, one end of the water absorption pad is aligned with the self-adhesive sticker 12, and the other end of the water absorption pad is overlapped and adhered to the reaction film. Removing the adhesive sticker 15, and sequentially adhering a labeled conjugate release pad and a sample pad, wherein one end of the labeled conjugate release pad is overlapped with the reaction membrane, and the other end of the labeled conjugate release pad is overlapped with the sample pad. The water absorption pad is pasted under the condition that the self-adhesive sticker 12 is not removed, the upper edge of the water absorption pad is aligned with the lower edge of the self-adhesive sticker 12, and the self-adhesive sticker 12 can play a role in pasting and positioning the water absorption pad. After the test strip shown in FIG. 5 is manufactured, the sticker 12 may or may not be removed. In another embodiment, after removing sticker 12, where barrier 8 is attached, and then removing sticker 13, the upper edge of the absorbent pad is attached along the lower edge of barrier 8. The blocking block 8 plays a role in positioning the pasting of the pasting absorbent pad.
Claims (10)
1. The detection test paper comprises a support plate, and a sample processing pad, a reaction membrane and a water absorption pad which are sequentially overlapped from upstream to downstream on the support plate, and is characterized in that a hydrophobic element is arranged at the downstream end of the water absorption pad of the detection test paper.
2. The test strip of claim 1, wherein the hydrophobic member is a front end of the support plate, the front end being made of a hydrophobic material and not covered by the absorbent pad.
3. The test strip of claim 1, wherein said hydrophobic element is a barrier.
4. The test strip of claim 3, wherein the stop is adhered to a support plate located downstream of the absorbent pad.
5. The test strip of claim 1, wherein said hydrophobic element is a handle.
6. The test strip according to one of claims 1 to 5, wherein said sample treatment pad comprises a separate sample pad and labeled conjugate release pad, which are adhered to the support plate in a stacked relationship from upstream to downstream.
7. The test strip of claim 6, wherein a sticker is attached to the sample treatment pad and the water absorption pad.
8. The test strip of claim 7, wherein the sticker is provided with a pinch indicator mark corresponding to the hydrophobic element.
9. The test strip according to any one of claims 1 to 5, wherein a detection line and a control line are provided on the reaction membrane.
10. The test strip of claim 9, wherein the hydrophobic element has an area sized to allow an operator's finger to hold the test strip without touching the absorbent pad.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
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CN202120565475.0U CN215575177U (en) | 2021-03-19 | 2021-03-19 | Detection test paper |
PCT/CN2022/081845 WO2022194296A1 (en) | 2021-03-19 | 2022-03-19 | Detection device |
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CN202120565475.0U CN215575177U (en) | 2021-03-19 | 2021-03-19 | Detection test paper |
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Cited By (1)
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WO2022194296A1 (en) * | 2021-03-19 | 2022-09-22 | 艾康生物技术(杭州)有限公司 | Detection device |
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CN102135499B (en) * | 2011-01-31 | 2014-08-13 | 艾康生物技术(杭州)有限公司 | Detecting device |
CN203101389U (en) * | 2013-03-22 | 2013-07-31 | 通辽天和世纪科技有限公司 | Fecal occult blood test paper |
CN206132777U (en) * | 2016-08-25 | 2017-04-26 | 中国人民解放军第四军医大学 | Detect test strip of lead content |
CN106771159B (en) * | 2016-12-01 | 2019-05-28 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of Florfenicol Rapid detection test strip |
CN110297087B (en) * | 2019-07-09 | 2022-09-16 | 津熙医学科技(上海)有限公司 | HPV antibody rapid detection test strip and preparation method and use method thereof |
CN211505569U (en) * | 2020-02-14 | 2020-09-15 | 信达安检测技术(天津)有限公司 | Colloidal gold test strip for detecting olaquindox metabolite |
CN215575177U (en) * | 2021-03-19 | 2022-01-18 | 艾康生物技术(杭州)有限公司 | Detection test paper |
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WO2022194296A1 (en) * | 2021-03-19 | 2022-09-22 | 艾康生物技术(杭州)有限公司 | Detection device |
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