CN101647852B - Veterinary medicine for enhancing immunologic function - Google Patents
Veterinary medicine for enhancing immunologic function Download PDFInfo
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Abstract
The invention relates to a veterinary medicine for enhancing an immunologic function, in particular to a traditional Chinese medicine immunopotentiator for animals, which has little toxic side effect and difficult medicine resistance generation, aiming at the current situation of livestock and poultries and especially aiming at immunity suppressive factors, and the traditional Chinese medicine immunopotentiator also has safety, high efficiency and controllable quality. The veterinary medicine for enhancing the immunologic function is characterized by comprising the following components in part (by weight): 9-18 parts of radix astragali, 6-9 parts of epimedium and 6-9 parts of glossy privet fruits. The invention can be prepared into various dosage forms, i.e. granules, oral liquid, injection liquid, troches, powder, and the like.
Description
Technical field
The present invention relates to a kind of immunostimulant, specifically a kind of veterinary drug of raise immunity.
Technical background
At present, disease works the mischief increasing to poultry (especially pig and chicken), new sick constantly appearance, the chronic illness sickness rate is high, and incidence is increasingly sophisticated, mixing/secondary infection ubiquity, and based on viral infection, immunity corresponding vaccine but the time morbidity arranged, the control difficulty is increasing, brings enormous economic loss for the production of poultry.
Infection phenomenon in the swinery of intensive farm is paid close attention to by people day by day, find pathogenic II type porcine circovirus (PCV-2) from the pig that suffers from multisystemic exhaustion syndrome after, many researchs have reported that PCV-2 can cause pmws (PMWS) especially.PCV-2 can cause B and the lymphocytic minimizing of T of pig and cause immunosuppressant.Simultaneously, PCV-2 can with other viral co-infected, as pig breeding and breathing syndrome virus (PRRSV), PRV (Pseudorabies virus) (ADV), pig parvoviral (PPV), thus the clinical symptoms and the pathological changes that increase the weight of to infect swinery.
Among the chicken group, immunosuppressive disease is also more and more common, the economic loss that is caused by it is on the rise, many viral infection all may cause chicken group immunosuppressive condition in various degree, as infectious bursal disease virus (IBDV), Marek's disease virus (MDV), RE hypertrophy virus (REV), Chicken Anemia Virus (CAV) (CIAV), reovirus (ReoV, infectious arthritis virus), fowl leukosis virus (ALV) etc.Can cause in the chicken group immunosuppressant viral infection in this class, these three kinds of viruses of RE hypertrophy virus, Chicken Anemia Virus (CAV) and infectious arthritis of chicken virus (exhaling the lonely ReoV of intestinal) can not only infect by contacting laterally, can also pass through the Embryo Gallus domesticus vertical infection.Chicken group's immunosuppressant virus infects the fertility performance that not only influences the chicken group, easilier cause multiple other bacillary and viral secondary infection, and easily cause specific vaccine immunity reaction inhibition, as cause that (Newcastle Disease Virus, NDV) vaccine antibody reaction weakens and make the potent antibodies titre persistent period short to Avian pneumo-encephalitis virus.
Except immunosuppressive disease, still there are mycotoxin, various stressors and nutrition, medicine or the like to cause that fowl poultry immune suppresses.
Chinese medicine is the various functions of conditioning human body on the whole, and the Chinese medicine immunostimulant can improve the non-specific immunity of body; And Chinese herbal medicine resource is abundant, and cheap, life-time service is little to the toxic and side effects of body, low-residual or noresidue, and therefore, the compound Chinese herb immunostimulant is one of development trend of animal immune potentiator in recent years.Application number is to have announced a kind of animal immunity potentiator in 200510029787.5 " Chinese herb compound animal immunity potentiator ", is made up of Chinese medicines such as Radix Bupleuri, the Radix Astragali, Radix Saposhnikoviae, Radix Codonopsis, Herba Epimedii, Folium Isatidis, Caulis Lonicerae, Radix Et Caulis Acanthopanacis Senticosi and humic acidss.But this compound preparation component is too many, complexity, and product quality is restive and drug effect is unstable.
Summary of the invention
The present invention is directed to the present situation of current livestock and poultry, particularly provide the veterinary drug that toxic and side effects is little, be difficult for producing chemical sproof raise immunity at the immunosuppressant sexual factor, and safe, efficient, quality controllable.
For this reason, the technical solution adopted in the present invention is: a kind of veterinary drug of raise immunity is characterized in that its recipe ingredient is by weight: 18 parts of the Radixs Astragali, 9 parts of Herba Epimedii, 9 parts of Fructus Ligustri Lucidi.
The present invention can be made into various dosage forms such as granule, oral liquid, injection, tablet, powder.
Compared with prior art, the present invention has following advantage:
1. it is raw material that safe the present invention selects the Radix Astragali, Herba Epimedii, Fructus Ligustri Lucidi for use, and these raw materials are natural Chinese medicine, has very long clinical practice history, toxic and side effects is little, noresidue, and these natural raw material of Chinese medicine and product thereof all can degrade in natural environment unlikelyly causes environmental pollution.
2. efficient the present invention, carries out the differential diagnosis in tcm opinion and controls prescription in conjunction with the research of fowl poultry immune damper according to Basic Theories of Chinese Medicine, meets the Chinese medicine feature, and product is through test, and effect is remarkable.
3. quality controllable product of the present invention is formulated target level of product quality, with astragaloside and icariin as thin layer chromatography, with icariin (C
33H
40O
15) meter, survey the finished product general flavone content, steady quality, controlled.
The specific embodiment
Embodiment 1: a kind of veterinary drug of raise immunity takes by weighing raw material (kg) by following proportioning: the Radix Astragali 18, Herba Epimedii 9, Fructus Ligustri Lucidi 9, make granule through extraction.
Preparation method: above three flavor Chinese medicines are decocted with water three times jointly, each 1.5 hours, collecting decoction, filter filtering and concentrating to 1: 1 (medical material: medicinal liquid), add 3 times of amount 95% ethanol, left standstill (4 ℃) 24 hours, filter, filtrate recycling ethanol, and be evaporated to the extractum that relative density is 1.30～1.35 (80 ℃); Get 1 part of extractum, add 3 parts of cane sugar powders, 1 part in dextrin, mixing is made granule, drying, promptly.
This product contains total flavones with icariin (C
33H
40O
15) meter, every 1g must not be less than 0.9mg.
Every 100g is equivalent to crude drug in whole 120g.
Embodiment 2: a kind of veterinary drug of raise immunity takes by weighing raw material (kg) by following proportioning: the Radix Astragali 15, Herba Epimedii 8, Fructus Ligustri Lucidi 8, make oral liquid through extraction.
Preparation method: above three flavor Chinese medicines are decocted with water three times jointly, each 1.5 hours, collecting decoction, filter filtering and concentrating to 1: 1 (medical material: medicinal liquid), add 3 times of amount 95% ethanol, left standstill (4 ℃) 24 hours, and filtered filtrate recycling ethanol, medicinal liquid adds the purified water dilution, transfers PH to 5.0-7.0, adds an amount of antiseptic, adding purified water is adjusted to every 1ml and is equivalent to crude drug in whole 1.2g, fill, sterilization, promptly.
This product contains total flavones with icariin (C
33H
40O
15) meter, every 1ml must not be less than 0.9mg.
Every 100ml is equivalent to crude drug in whole 120g.
Embodiment 3: a kind of veterinary drug of raise immunity takes by weighing raw material (kg) by following proportioning: the Radix Astragali 12, Herba Epimedii 7, Fructus Ligustri Lucidi 7, make injection through extraction.
Preparation method: above three flavor Chinese medicines are decocted with water three times jointly, and each 1.5 hours, collecting decoction filtered, filtering and concentrating to 1: 1 (medical material: medicinal liquid), add 3 times of amount 95% ethanol, left standstill (4 ℃) 24 hours, filter, filtrate recycling ethanol, medicinal liquid add 5 times of amount 95% ethanol, leave standstill (4 ℃) 24 hours, filter, filtrate recycling ethanol adds the injection dilute with water, transfer PH to 5.0-7.0, add the injection water again and be adjusted to every 1ml and be equivalent to crude drug in whole 1.2g, filter, fill, sterilization, promptly.
This product contains total flavones with icariin (C
33H
40O
15) meter, every 1ml must not be less than 0.9mg.
Every 100ml is equivalent to crude drug in whole 120g.
Embodiment 4: a kind of veterinary drug of raise immunity takes by weighing raw material (kg) by following proportioning: the Radix Astragali 9, Herba Epimedii 6, Fructus Ligustri Lucidi 6, make granule according to the preparation method of embodiment 1, and the content of flavone is identical with embodiment 1 in the finished product.
Now the veterinary drug granule with the raise immunity of embodiment 1 is an example, is that animal model proves its potentiation to the animal immune ability by experiment with the chicken, and embodiment 2-4 has close effect through similarly experimental results show that.
(1) materials and methods
1. material and source
1.1 be subjected to the reagent thing:
1.1.1 title: the veterinary drug granule of raise immunity provided by the invention
1.1.2 research institute: the greatly healthy animal health product company limited of Zhejiang gold
1.1.3 specification: 100g/ bag.
1.2 control drug
1.2.1 title: astragalus polysaccharides oral liquid
1.2.2 production unit: the precious refreshing animal health product company limited of Guangzhou white clouds
1.2.3 lot number: 050901
1.2.4 specification: 100ml contains the 100g crude drug
2. experimental animal
The young broiler of 2.1 kind: Ha Bade
2.2 source: available from Ningbo positive multi-form agriculture company limited kind chicken house
2.3 age in days: 1 age in days is raised to 6 ages in days
2.4 every treated animal number: 30
2.5 raising condition: raise in the laboratory of strict sterilization, the 731 chicken particulate material of feeding (Guangdong Zhejiang Hengxing Feedstuff Co., Ltd of permanent emerging group) are drunk clean tap water.
3. test is divided into groups and route of administration
150 6 ages in days are breathed out the young broiler of the white plumage of Ahmedabad by the numbering of only weighing on an empty stomach, are divided into 5 groups at random according to the body weight dispersion, 30 every group.Began administration the same day, the equal by specification eye drip inoculation of 5 groups of test chickens next day (7 age in days) newcastle disease (ND) IV is a vaccine.
3.1 drug test group: establish and be subjected to reagent object height dosage group, middle dosage group and three groups of low dose group.
Low dose group: the each 0.1g of every plumage, 2 times on the one, drinking-water administration, logotype 5 days;
Middle dosage group: the each 0.15g of every plumage, 2 times on the one, drinking-water administration, logotype 5 days;
High dose group: the each 0.2g of every plumage, 2 times on the one, drinking-water administration, logotype 5 days.
3.2 astragalus polysaccharides oral liquid medicine matched group: the each 0.1ml of every plumage, 2 times on the one, drinking-water administration, logotype 5 days.
3.3 blank group: do not add any medicine.
Test grouping and route of administration concrete condition see Table 1.
Table 5-1 test grouping and processing
4. observation index and detection method
4.1ND-HI antibody (newcastle-hemagglutination inhibition antibody) detects respectively at after the immunity the 7th day and the 21st day, random choose 10 plumages, wing venous blood collection 1ml, separation of serum adopts " U " Microhemagglutination plate to suppress (HI) test with the newcastle disease blood clotting and detects the ND-HI antibody titer.
The assay method of antibody titer: add diluent (0.01mol/LpH7.2PBS) 50 μ l in each hole of " U " type Sptting plate.Get serum 50 μ l to be checked,, make doubling dilution successively, reject mixed liquor 50 μ l to last hole since first hole.Every hole adds 1: 16 newcastle disease standard antigen diluent 50 μ l respectively; Simultaneously, establish newcastle disease standard antigen, positive serum (all available from the anti-inspection of Zhejiang Province poultry station), three contrasts of diluent, on micro oscillator, shake up, put 37 ℃ of placement 30min in the incubator.Every hole adds 1% chicken erythrocyte suspension, 50 μ l again, and mixing is put 37 ℃ and observed after placing 30min.
4.2 the calculating of immune organ body-mass index after immunity the 7th day, random choose chicken 10 plumages after the carotid artery blood-letting causes death, are gathered spleen and two kinds of immune organs of fabricius bursa, take by weighing weight in wet base, calculate the immune organ index by following formula.
Spleen index=spleen weight in wet base (mg)/chicken live body heavy (g).
Fabricius bursa index=fabricius bursa weight in wet base (mg)/chicken live body heavy (g).
5. data statistics and therapeutic evaluation
Utilization t check is each processed group and blank group relatively, is subjected to reagent thing test group and astragalus polysaccharides matched group, and is subjected to the significance of difference between reagent thing various dose group.
(2) result of the test and analysis
1. to the influence of newcastle disease HI antibody titer
ND-HI antibody titer measurement result sees Table 2 in each test group chicken serum.
ND-HI antibody titer measurement result in each test group chicken serum of table 2 (log2, X ± SD)
Annotate: compare * P＜.05 and * * P＜.01 with the blank group; Compare with the astragalus polysaccharides matched group,
aP＜.05 and
AaP＜0.01.
2. to the exponential influence of immune organ
Each test group chicken immune organ assessment of indices the results are shown in Table 3.
Each test group chicken immune organ assessment of indices result (mg/g) of table 3 (X ± SD)
Annotate: compare * P＜0.05 and * * P＜0.01 with the blank group.
3. interpretation of result
The ND-HI antibody titer is analyzed through the t inspection statistics in each test group chicken serum.The result shows: immunity back the 7th day is subjected to that the ND-HI antibody titer utmost point is significantly higher than blank group (P＜0.01) in three dosage groups of reagent thing and the astragalus polysaccharides medicine matched group chicken serum.Be subjected between three dosage groups of reagent thing and the astragalus polysaccharides medicine matched group in the serum ND-HI antibody titer there are no significant difference (P＞0.05%).Be subjected in three test group chicken serums of reagent thing between the ND-HI antibody titer difference not significantly (P＞0.05).Immunity back the 21st day is subjected to that the ND-HI antibody titer utmost point is significantly higher than blank group and astragalus polysaccharides medicine matched group (P＜0.01) in three dosage groups of reagent thing chicken serum.Between the ND-HI antibody titer, the highest with middle dosage group, high dose group is taken second place in three dosetest group chicken serums, and low dose group is minimum, and each group difference is (P＜0.05 or P＜0.01) significantly or extremely significantly.
Adopt the t check to each test group chicken spleen exponential sum fabricius bursa index statistical analysis, immunity back the 7th day, be subjected to the spleen index of dosetest group in the reagent thing, high dose test group and astragalus polysaccharides oral liquid medicine matched group chicken and fabricius bursa index all extremely significantly greater than blank group (P＜0.01), and be subjected between reagent thing low dosage test group and the blank group difference not significantly (P＞0.05).The difference (P＞0.05) that is subjected to that there are no significant between three test group of reagent thing and the astragalus polysaccharides medicine matched group.Be subjected between three dosage groups of reagent thing, bigger with average spleen index and the fabricius bursa index of middle dosage group chicken, but difference not significantly (P＞0.05) between each group.
4. untoward reaction and anti symptom treatment
Do not find untoward reaction in this process of the test.
(3) conclusion
1. being subjected to the reagent thing can strengthen chicken is the immunne response of attenuated vaccine to newcastle (ND) IV, have significant immunological enhancement, and effect is better than astragalus polysaccharides.
2. clinical recommendation using dosage: use by per kilogram of body weight 0.15g, or convert water 100L, freely drink water logotype 3～5 days by 100g.
At last, it is also to be noted that what more than enumerate only is some specific embodiments of the present invention.If those of ordinary skills from all distortion that content disclosed by the invention directly derives or associates, all should think protection scope of the present invention.
Claims (2)
1. the veterinary drug of a raise immunity is characterized in that its recipe ingredient is by weight: 18 parts of the Radixs Astragali, 9 parts of Herba Epimedii, 9 parts of Fructus Ligustri Lucidi.
2. the veterinary drug of raise immunity according to claim 1, it is characterized in that: its dosage form is granule, oral liquid, injection, tablet or powder.
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Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| ITBO20120123A1 (en) * | 2012-03-12 | 2013-09-13 | Ri Mos S R L | COMPOSITION CONTAINING ALLIUM EXTRACTS AND NEEM OIL WITH ANTIMICROBIAL, ANTIBACTERIAL, ANTISEPTIC, ANTIFUNGAL, REPELLENT, CICATRIZING AND TISSUE REGENERATION |
| CN103535529B (en) * | 2013-10-31 | 2015-03-04 | 青岛施维雅生物制药有限公司 | Traditional Chinese medicine feed additive, preparation method of additive, and feed containing additive |
| CN103655728A (en) * | 2013-12-18 | 2014-03-26 | 江苏恒丰强生物技术有限公司 | Long-acting Qizhen immunity-enhancing granule pill |
| CN104800293A (en) * | 2015-05-14 | 2015-07-29 | 胡少平 | Veterinary codonopsis pilosula and astragalus immunity improving Chinese medicine preparation |
| CN105816500A (en) * | 2016-04-12 | 2016-08-03 | 镇江荣邦生物科技有限公司 | Pig epimedium herb granule preparation process |
| CN107041902A (en) * | 2016-10-31 | 2017-08-15 | 四川美嘉龙生物科技有限公司 | A kind of stilbene chastity, which increases, exempts from particle and preparation technology |
| CN108452022A (en) * | 2017-06-28 | 2018-08-28 | 禹州市合同泰药业有限公司 | A kind of veterinary drug of enhancing immunity of poultry |
| CN107927343A (en) * | 2017-11-30 | 2018-04-20 | 南宁学院 | A kind of additive for preventing chicken infectious anemia |
| CN109010442A (en) * | 2018-08-23 | 2018-12-18 | 河北普德动物药业有限公司 | Membrane separation technique increases the application exempted from oral liquid preparing process in loyal lotus |
| CN110882304A (en) * | 2019-12-02 | 2020-03-17 | 广东容大生物股份有限公司 | Method for preparing astragalus root and glossy privet fruit medicine for livestock and poultry |
| CN114010684A (en) * | 2021-12-21 | 2022-02-08 | 江西利德菲生物药业有限公司 | Astragalus membranaceus and glossy privet immunity-enhancing granule and preparation method thereof |
| CN114848711A (en) * | 2022-06-13 | 2022-08-05 | 沈阳伟嘉生物技术有限公司 | Pharmaceutical composition containing astragalus and glossy privet immunopotentiating particle material basis and preparation method and application thereof |
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2009
- 2009-08-28 CN CN2009101018694A patent/CN101647852B/en active Active
Non-Patent Citations (1)
| Title |
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| 石达友等.中药提取物对鸡免疫器官发育和新城疫抗体水平的影响.《中兽医学杂志》.2004,(第2期),第4-5页. * |
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Effective date of registration: 20210308 Address after: 321000 No. 360, Binhong West Road, Wucheng District, Jinhua City, Zhejiang Province Patentee after: ZHEJIANG DKCOM ANIMAL HEALTH PRODUCTS Co.,Ltd. Address before: 16, No. 70, 321016 Rainbow Road, Jiangnan street, Wucheng District, Zhejiang, Jinhua Patentee before: Miao Xuerong |