CN101637097A - Quick and high-efficiency cephalotaxus hainanensis seed germinating and high-quality seedling breeding method - Google Patents
Quick and high-efficiency cephalotaxus hainanensis seed germinating and high-quality seedling breeding method Download PDFInfo
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- CN101637097A CN101637097A CN200910184213A CN200910184213A CN101637097A CN 101637097 A CN101637097 A CN 101637097A CN 200910184213 A CN200910184213 A CN 200910184213A CN 200910184213 A CN200910184213 A CN 200910184213A CN 101637097 A CN101637097 A CN 101637097A
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Abstract
The invention provides a quick and high-efficiency cephalotaxus hainanensis seed germinating and high-quality seedling breeding method which relates to a quick and high-efficiency plant seed germinating and high-quality seedling breeding method. The method comprises the following steps: (1) seed collection: collecting cephalotaxus hainanensis seeds to be matured or matured seeds; (2) preparation of seed germinating matrixes; (3) release of seed dormancy; (4) seed germination; and (5) acclimatization and transplantation. The invention can enhance the seed germinating bud ratio by more than 85%,the seedling viability exceeds 90%, the uniform high-quality cephalotaxus hainanensi seedlings can be produced, and the viability of seeds transplanted to fields can also exceed 90%; compared with the traditional method, the stocking time is shortened by about 2/3; the related facilities and processes of tissue culture and the like are not required, so the operation is convenient; the invention provides technical support for the quick propagation and restoration protection of rare or endangered species of cephalotaxus hainanensis and the development and the utilization of resources.
Description
Technical field
The present invention relates to a kind of plant seed and rapidly and efficiently sprout the method for cultivating, relate in particular to the method that high quality seedling is rapidly and efficiently sprouted and cultivated to the Cephalotaxus mannii seed with high quality seedling.
Background technology
Cephalotaxus mannii (Cephalotaxus hainanensis Li) is the Cephalotaxaceae plant, and another name cephalotaxus hainanensis, slender lobule bamboo comb China fir are perennial tall and big aiphyllium.Be the distinctive rare tree species of China, its distribution is very narrow, is detected in some areas such as Hainan and Guangdong, Yunnan.Studies show that the not only effective alkaloid of Cephalotaxus mannii is the highest in the Cephalotaxaceae, and curative effect is the most remarkable, is one of best anticancer seeds of generally acknowledging at present.These seeds are cut down repeatly because of material is good in addition, and population quantity is few in addition, are one of most important rare or endangered species of China.
But this seeds natural resources is very limited, is difficult to satisfy growing demand.Because these seeds are dioecism, seed production very low (the only tens of grains of annual every female plant); Mature seed has hibernation feature, can not germinate after landing at once, arrive next year and even the 3rd year spring could germinating to seedling as can not directly touching soil, most of seed loses vigor gradually between resting stage and rots.So, form seedling by seed under field conditions (factors) and treelet is few.Even adopt the artificial germination method of tradition; its germination rate about 20%; and traditional artificial germination method needs about 1 year could Cheng Miao; the seedling growing way that traditional in addition artificial germination method is bred is poor, and vitality is not strong, lethality also very high (reaching 70%); be subjected to factor such as its ecological and artificial destruction in addition and cause the endangered (Wang Xianpu of Cephalotaxus mannii; Wang Yousheng. reason that Cephalotaxus mannii is in imminent danger and safeguard measure. GUIHAIA, 1994,14 (4): 369-372).Though also the someone studies the employing cottage propagation, but under the endangered situation of germ plasm resource, provide the difficulty of a large amount of nursery stocks very big by cuttage, do not satisfy the demand of pharmaceutical industry far away, and can cause bigger destruction the limited resources of Cephalotaxus mannii to Cephalotaxus mannii; And data shows that cuttage tender stem segments source directly has influence on cuttage growth of seedlings situation, and only deriving from the seedling that the young shoot cuttage on stem top produces can normal upright well grow; And stem top tender stem segments is drawn materials and can directly be destroyed the normal growth of this strain plant.So, cottage propagation can not promote substantially (Fu Wenying, Pan Xuefeng, Chen Xiuhui, Liu its people, Li Renzhu. the Cephalotaxus mannii cuttage and the experimental study of taking root. University Of Hainan's journal (natural science edition), 1997,15 (3): 239-244; Pan Xuefeng, Fu Wenying, Chen Xiuhui, Liu its people, Li Renzhu. the research of Cephalotaxus mannii cottage propagation. tropical agriculture science, 1996, (3): 25-30; Du Daolin, Fu Wenying. rare or endangered species Cephalotaxus mannii population protection bioecology. Hunan science and technology publishing house, 2003.11).Also the someone studies and adopts traditional method for tissue culture that Cephalotaxus mannii is carried out the seedling breeding in addition, but can not emerge substantially, perhaps emergence rate is very low, can not carry out application (Fu Wenying, Du Daolin, tally is equal. the inducing and cultivating of Cephalotaxus mannii callus. and Plant Physiology Communications, 2004,40 (1): 34-36; Li Zhiying, Wang Zhunian, Xu Li. the breeding fast of exsomatizing of Cephalotaxus mannii. Plant Physiology Communications, 2005,41 (5): 786).
Given this, the species conservation of Cephalotaxus mannii causes the great attention of botany research institution.So; the invention provides a kind of straightforward procedure of rapidly and efficiently breeding the system of high quality seedling with the Cephalotaxus mannii seed; specifically comprise following operating procedure: seed collection; seed germination and growth of seedling matrix are prepared; seed dormancy is removed; seed germination; domestication transplanting etc.; can realize improving more than the seed germination bud ratio to 85%; seedling survives and surpasses 90%; and the high-quality Cephalotaxus mannii seedling of production neat and consistent; sprigging also surpasses 90% to open-air survival rate, and it is about 2/3 to become seedling time ratio conventional method to shorten, and need not organize related facility and process such as training; operate very easyly, provide technical guarantee for solving fast numerous and recovery and protection of rare or endangered species Cephalotaxus mannii high quality seedling and resources development and utilization.
Summary of the invention
The key technical problem that the present invention solves is the systems approach of directly rapidly and efficiently breeding high quality seedling with the Cephalotaxus mannii seed; specifically comprise seed collection; seed germination and growth of seedling matrix are prepared; seed dormancy is removed; seed germination; operating procedures such as domestication transplanting; can improve more than the seed germination bud ratio to 85%; seedling survives and surpasses 90%; and the high-quality Cephalotaxus mannii seedling of production neat and consistent; sprigging also surpasses 90% to open-air survival rate; become seedling time ratio conventional method to shorten about 2/3; the most important thing is to organize training and wait related facility and process, operate very easyly, the rare or endangered species Cephalotaxus mannii is fast numerous to provide technical guarantee with recovery and protection and resources development and utilization in order to solve.
The technical problem to be solved in the present invention is to be brood body with the Cephalotaxus mannii seed, and a whole set of Cephalotaxus mannii high quality seedling method for quickly breeding is provided.The technical scheme that addresses the above problem comprises the steps:
(1) seed collection: collect and grow near ripe or ripe Cephalotaxus mannii seed;
(2) seed germination matrix is prepared: seed germination matrix is about soil and the face of land humus of collecting from open-air Cephalotaxus mannii group: coconut palm chaff=1-3: 1 mixes;
(3) seed dormancy is removed: the approaching ripe or ripe Cephalotaxus mannii seed of the growth that will collect is at 0.1-1mM HCl (hydrochloric acid) or H
2SO
4(sulfuric acid) or C
2H
4O
2(oxalic acid) normal temperature (about 25 ℃) washes 3-6 time repeatedly with sterile water after soaking about 16-32h.; Place about 1-3 week of low-temperature preservation of 1-10 ℃ then, remove Seed Dormancy;
(4) seed germination: the seed of breaking dormancy is broadcast into ready prepd seed germination matrix, and normal temperature is sprouted (18-35 ℃); About 3-5 week just obtains the seedling that root is arranged of new green, and emergence rate surpasses 85%;
(5) domestication is transplanted: seedling is moved to new matrix (sprouting matrix with seed) make basin seedling or bag seedling, seedling percent reaches more than 90%; 8-12 is after week in growth, can transplant in suitable in the open air area, and survival rate surpasses 90%.
The invention has the beneficial effects as follows: the systems approach of directly rapidly and efficiently breeding high quality seedling with the Cephalotaxus mannii seed; improve more than the seed germination bud ratio to 85%; seedling survives and surpasses 90%; and the high-quality Cephalotaxus mannii seedling of production neat and consistent; sprigging also surpasses 90% to open-air survival rate; become seedling time ratio conventional method to shorten about 2/3; and need not organize related facility and processes such as training; operate very easyly, provide technical guarantee for solving fast numerous and recovery and protection of rare or endangered species Cephalotaxus mannii and resources development and utilization.
Embodiment
The specific embodiment of the invention is illustrated below in conjunction with example: required various medicine and reagents all are commercially available, and press the adapted of conventional method shown in the catalogue, by industry general.
Embodiment 1:
(1) seed collection: collect and grow near 20 in ripe or ripe Cephalotaxus mannii seed;
(2) seed germination matrix is prepared: soil and the face of land humus of seed germination matrix for collecting from open-air Cephalotaxus mannii group: coconut palm chaff=mix at 1: 1;
(3) seed dormancy is removed: the growth that will collect is washed 3 times with sterile water after 0.5mM HCl normal temperature (25 ℃) soaks 32h. repeatedly near ripe or ripe Cephalotaxus mannii seed; , place 1 week of low-temperature preservation of 1 ℃ then, remove Seed Dormancy;
(4) seed germination: the seed of breaking dormancy is broadcast into ready prepd seed germination matrix, and normal temperature is sprouted (18 ℃); 5 weeks obtained the seedling that root is arranged 19 strains of new green, and emergence rate surpasses 95%;
(5) domestication is transplanted: 19 strain seedling are moved to new matrix (sprouting matrix with seed) make the basin seedling, seedling percent reaches 100%; Grew for 8 weeks, be transplanted to the suitable area of Hainan Ba Wangling, check after half a year, 18 strains survive, survival rate about 95%.
Embodiment 2:
(1) seed collection: collect and grow near 20 in ripe or ripe Cephalotaxus mannii seed;
(2) seed germination matrix is prepared: soil and the face of land humus of seed germination matrix for collecting from open-air Cephalotaxus mannii group: coconut palm chaff=mix at 3: 1;
(3) seed dormancy is removed: the approaching ripe or ripe Cephalotaxus mannii seed of the growth that will collect is at 1mM C
2H
4O
2Normal temperature (25 ℃) washes 6 times repeatedly with sterile water after soaking 16h.; Place 10 ℃ 3 weeks of low-temperature preservation then, remove Seed Dormancy;
(4) seed germination: the seed of breaking dormancy is broadcast into ready prepd seed germination matrix, and normal temperature is sprouted (35 ℃); 3 weeks obtained the seedling that root is arranged 19 strains of new green, and emergence rate surpasses 95%;
(5) domestication is transplanted: 19 strain seedling are moved to new matrix (sprouting matrix with seed) make the basin seedling, seedling percent reaches 100%; Grew for 8 weeks, be transplanted to the suitable area of Hainan Ba Wangling, check after half a year, 19 strains survive, survival rate 100%.
Claims (1)
1, the method for high quality seedling is rapidly and efficiently sprouted and cultivated to the Cephalotaxus mannii seed, it is characterized in that carrying out according to following step:
(1) seed collection: collect and grow near ripe or ripe Cephalotaxus mannii seed;
(2) seed germination matrix is prepared: soil and the face of land humus of seed germination matrix for collecting from open-air Cephalotaxus mannii group: coconut palm chaff=1-3: 1 mixes;
(3) seed dormancy is removed: the growth that will collect, is washed 3-6 time with sterile water after 25 ℃ of normal temperature soak 16-32h. at 0.1-1mM hydrochloric acid or sulfuric acid or oxalic acid repeatedly near ripe or ripe Cephalotaxus mannii seed; Place about 1-3 week of low-temperature preservation of 1-10 ℃ then, remove Seed Dormancy;
(4) seed germination: the seed of breaking dormancy is broadcast into ready prepd seed germination matrix, 18-35 ℃ of sprouting; 3-5 week just obtains the seedling that root is arranged of new green, and emergence rate surpasses 85%;
(5) domestication is transplanted: seedling is moved to new matrix make basin seedling or bag seedling, seedling percent reaches more than 90%, and the composition of new matrix is sprouted matrix with seed; 8-12 is after week in growth, can transplant in suitable in the open air area, and survival rate surpasses 90%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101842133A CN101637097B (en) | 2009-08-14 | 2009-08-14 | Quick and high-efficiency cephalotaxus hainanensis seed germinating and high-quality seedling breeding method |
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| CN101637097A true CN101637097A (en) | 2010-02-03 |
| CN101637097B CN101637097B (en) | 2011-08-10 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897294A (en) * | 2010-06-22 | 2010-12-01 | 福建农林大学 | Seed selection method for sweet potato seedling of two generators in one year |
| CN102283122A (en) * | 2011-07-11 | 2011-12-21 | 镇江瑞繁农艺有限公司 | Dormancy breaking seeding raising method for water lily seeds |
| CN102612969A (en) * | 2012-04-09 | 2012-08-01 | 常熟市海虹苗木花卉有限公司 | Macrocarpium officinalis seedling raising method |
| CN104969691A (en) * | 2015-08-06 | 2015-10-14 | 吴小萍 | Method for promoting germination of cinnamon seeds exceeding time limit of germination |
| CN107771672A (en) * | 2017-11-08 | 2018-03-09 | 江苏省中国科学院植物研究所 | A kind of method for obtaining Hibiscus hamabo aseptic seedling |
| CN108668558A (en) * | 2018-04-19 | 2018-10-19 | 边学静 | A method of abolishing Cephalotaxus mannii seed dormancy |
| CN113228872A (en) * | 2021-05-28 | 2021-08-10 | 西南科技大学 | Method for repairing acidic black rock stratum slope |
-
2009
- 2009-08-14 CN CN2009101842133A patent/CN101637097B/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897294A (en) * | 2010-06-22 | 2010-12-01 | 福建农林大学 | Seed selection method for sweet potato seedling of two generators in one year |
| CN101897294B (en) * | 2010-06-22 | 2012-04-11 | 福建农林大学 | Seed selection method for sweet potato seedling of two generators in one year |
| CN102283122A (en) * | 2011-07-11 | 2011-12-21 | 镇江瑞繁农艺有限公司 | Dormancy breaking seeding raising method for water lily seeds |
| CN102283122B (en) * | 2011-07-11 | 2013-02-27 | 镇江瑞繁农艺有限公司 | Dormancy breaking seeding raising method for water lily seeds |
| CN102612969A (en) * | 2012-04-09 | 2012-08-01 | 常熟市海虹苗木花卉有限公司 | Macrocarpium officinalis seedling raising method |
| CN102612969B (en) * | 2012-04-09 | 2013-04-17 | 常熟市海虹苗木花卉有限公司 | Macrocarpium officinalis seedling raising method |
| CN104969691A (en) * | 2015-08-06 | 2015-10-14 | 吴小萍 | Method for promoting germination of cinnamon seeds exceeding time limit of germination |
| CN107771672A (en) * | 2017-11-08 | 2018-03-09 | 江苏省中国科学院植物研究所 | A kind of method for obtaining Hibiscus hamabo aseptic seedling |
| CN108668558A (en) * | 2018-04-19 | 2018-10-19 | 边学静 | A method of abolishing Cephalotaxus mannii seed dormancy |
| CN113228872A (en) * | 2021-05-28 | 2021-08-10 | 西南科技大学 | Method for repairing acidic black rock stratum slope |
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