CN101629204A - 在缺乏酶的黑曲霉突变体中生产生物物质的方法 - Google Patents

在缺乏酶的黑曲霉突变体中生产生物物质的方法 Download PDF

Info

Publication number
CN101629204A
CN101629204A CN200910167050A CN200910167050A CN101629204A CN 101629204 A CN101629204 A CN 101629204A CN 200910167050 A CN200910167050 A CN 200910167050A CN 200910167050 A CN200910167050 A CN 200910167050A CN 101629204 A CN101629204 A CN 101629204A
Authority
CN
China
Prior art keywords
ala
enzyme
aspergillus niger
leu
ser
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN200910167050A
Other languages
English (en)
Chinese (zh)
Inventor
玛丽亚·康奈利
霍华德·布罗迪
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novozymes Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biotech Inc filed Critical Novozymes Biotech Inc
Publication of CN101629204A publication Critical patent/CN101629204A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/40—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Candida
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
CN200910167050A 2003-03-31 2004-03-31 在缺乏酶的黑曲霉突变体中生产生物物质的方法 Pending CN101629204A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45990203P 2003-03-31 2003-03-31
US60/459,902 2003-03-31

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CNB2004800151129A Division CN100547078C (zh) 2003-03-31 2004-03-31 在缺乏酶的黑曲霉突变体中生产生物物质的方法

Publications (1)

Publication Number Publication Date
CN101629204A true CN101629204A (zh) 2010-01-20

Family

ID=33159708

Family Applications (2)

Application Number Title Priority Date Filing Date
CN200910167050A Pending CN101629204A (zh) 2003-03-31 2004-03-31 在缺乏酶的黑曲霉突变体中生产生物物质的方法
CNB2004800151129A Expired - Lifetime CN100547078C (zh) 2003-03-31 2004-03-31 在缺乏酶的黑曲霉突变体中生产生物物质的方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CNB2004800151129A Expired - Lifetime CN100547078C (zh) 2003-03-31 2004-03-31 在缺乏酶的黑曲霉突变体中生产生物物质的方法

Country Status (8)

Country Link
US (3) US7303877B2 (enExample)
EP (1) EP1613761B1 (enExample)
JP (1) JP4638418B2 (enExample)
CN (2) CN101629204A (enExample)
AT (1) ATE441664T1 (enExample)
DE (1) DE602004022929D1 (enExample)
DK (1) DK1613761T3 (enExample)
WO (1) WO2004090155A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114736880A (zh) * 2022-06-09 2022-07-12 中国农业科学院北京畜牧兽医研究所 酸稳定性提高葡萄糖氧化酶GoxM10的突变体D497N及其衍生突变体和应用

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101094919B (zh) * 2004-10-12 2014-09-03 帝斯曼知识产权资产管理有限公司 在生产多肽的方法中有用的真菌转录活化因子
DK1831384T3 (en) 2004-12-22 2015-11-23 Novozymes As Polypeptides with glucoamylase activity and polynucleotides encoding them.
JP2008527985A (ja) 2005-01-24 2008-07-31 ディーエスエム アイピー アセッツ ビー．ブイ． 糸状菌細胞において目的の化合物を産生させるための方法
CA2630455A1 (en) 2005-11-29 2007-06-07 Dsm Ip Assets B.V. Dna binding site of a transcriptional activator useful in gene expression
EP2035561A1 (en) 2006-06-29 2009-03-18 DSMIP Assets B.V. A method for achieving improved polypeptide expression
WO2008053018A2 (en) 2006-11-02 2008-05-08 Dsm Ip Assets B.V. Improved production of secreted proteins by filamentous fungi
AU2008214663B2 (en) 2007-02-15 2013-10-03 Dsm Ip Assets B.V. A recombinant host cell for the production of a compound of interest
ES2490608T3 (es) 2007-12-06 2014-09-04 Novozymes A/S Polipéptidos con actividad de esterasa de acetilxilano y polinucleótidos que codifican los mismos
WO2010108918A1 (en) 2009-03-24 2010-09-30 Novozymes A/S Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
CN102414323B (zh) 2009-04-22 2015-07-08 帝斯曼知识产权资产管理有限公司 用于生产感兴趣的重组多肽的方法
WO2010122141A1 (en) 2009-04-24 2010-10-28 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
BR122018077148B1 (pt) * 2009-12-18 2020-11-03 Novozymes, Inc mutante de uma cepa de trichoderma reesei parental isolada, e, métodos para produzir um polipeptídeo de inetresse e para obter um mutante de uma cepa de trichoderma reesei parental
CN102762727B (zh) 2010-02-11 2016-07-20 帝斯曼知识产权资产管理有限公司 能产生用于降解木质纤维素材料的酶的宿主细胞
CN102834511A (zh) 2010-02-11 2012-12-19 帝斯曼知识产权资产管理有限公司 具有纤维二糖水解酶活性的多肽及其用途
CN101906393B (zh) * 2010-06-28 2012-07-25 江南大学 一种谷氨酰胺转胺酶编码基因阻断吸水链霉菌及其应用
CN102971419B (zh) 2010-06-29 2015-02-11 帝斯曼知识产权资产管理有限公司 具有降解碳水化合物材料的活性或帮助降解碳水化合物材料的活性的多肽及其用途
CA2803982A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
US9121013B2 (en) 2010-06-29 2015-09-01 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
CN103180337A (zh) 2010-06-29 2013-06-26 帝斯曼知识产权资产管理有限公司 具有膨胀因子活性的多肽及其用途
BR112012033701A2 (pt) 2010-06-29 2018-08-28 Dsm Ip Assets Bv polipeptídeo tendo atividade de degradação de carbohidrato e seus usos
CN103391999B (zh) 2010-06-29 2016-06-22 帝斯曼知识产权资产管理有限公司 具有乙酰基木聚糖酯酶活性的多肽及其用途
EP2625269A2 (en) 2010-10-06 2013-08-14 BP Corporation North America Inc. Variant cbh i polypeptides with reduced product inhibition
US20120276247A1 (en) 2011-04-29 2012-11-01 Uwe Bornscheuer Lipase Variants
JP5846476B2 (ja) * 2011-07-08 2016-01-20 独立行政法人酒類総合研究所 微生物のコウジ酸生産性を向上させる方法
CN102296032B (zh) * 2011-08-31 2013-11-06 保龄宝生物股份有限公司 转葡糖苷酶及其制备和固定化方法
GB2508569A (en) * 2011-11-02 2014-06-04 Univ Putra Malaysia A method for producing a biopolymer
DK2800809T3 (en) 2012-01-05 2018-05-22 Glykos Finland Oy PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
US9695454B2 (en) 2012-05-23 2017-07-04 Glykos Finland Oy Production of fucosylated glycoproteins
EP2885409A1 (en) 2012-08-17 2015-06-24 Novozymes A/S Methods for co-silencing expression of genes in filamentous fungal strains and uses thereof
JP2016523552A (ja) 2013-07-10 2016-08-12 ノバルティス アーゲー 多重プロテアーゼ欠損糸状菌細胞及びその使用方法
DK3036324T3 (en) 2013-08-23 2019-04-01 Novozymes As Regulated PepC expression
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
US10550398B2 (en) 2016-04-19 2020-02-04 Novozymes A/S RlmA-inactivated filamentous fungal host cell
AU2017380488B2 (en) 2016-12-21 2023-09-14 Novozymes A/S Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
CN108504615B (zh) * 2018-03-30 2020-12-29 江南大学 一种产酸性蛋白酶的重组菌及其应用
CA3116718A1 (en) * 2018-10-17 2020-04-23 Perfect Day, Inc. Recombinant components and compositions for use in food products
EP4004187A1 (en) * 2019-07-26 2022-06-01 Novozymes A/S Modified filamentous fungal host cell
CN113265417B (zh) * 2020-02-14 2025-01-21 中国科学院天津工业生物技术研究所 有机酸产量提高的菌株及其构建方法和应用
EP4228424A1 (en) 2020-10-15 2023-08-23 DSM IP Assets B.V. Methods of modulating gastrointestinal metabolites
PE20251535A1 (es) 2022-05-14 2025-06-05 Novozymes As Composiciones y metodos para prevenir, tratar, suprimir y/o eliminar infecciones e infestaciones fitopatogenas
WO2023242273A1 (en) * 2022-06-14 2023-12-21 Newmilkbuzz B.V. Expression of milk caseins in filamentous fungi
WO2025230415A1 (en) 2024-04-30 2025-11-06 Ginkgo Bioworks Netherlands B.V. Host cells expressing optb protein variants and uses thereof
CN119265158B (zh) * 2024-09-27 2025-11-04 湖北大学 一种脂肪酶a突变体及其制备方法和应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5536661A (en) 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5252726A (en) * 1987-09-04 1993-10-12 Novo Nordisk A/S Promoters for use in aspergillus
DK0531372T4 (da) 1990-05-09 2004-08-09 Novozymes As Cellulasepræparat omfattende et endoglucanaseenzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
EP0765394B1 (en) 1994-06-03 2001-10-04 Novo Nordisk Biotech, Inc. Purified myceliophthora laccases and nucleic acids encoding same
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5646025A (en) 1995-05-05 1997-07-08 Novo Nordisk A/S Scytalidium catalase gene
BR9714010A (pt) * 1996-12-12 2000-05-09 Leopold Flohe Triparredoxina, plasmìdeo de expressão, processo para produção, uso, sistema de teste e preparado farmacêutico
JP5303084B2 (ja) * 1997-04-11 2013-10-02 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ 工業的組み換え生物を溝築するための手段としての遺伝子変換
US6432371B1 (en) * 1998-09-18 2002-08-13 Jack Oliver, Jr. Child proof chlorine dispenser with a three dimensional ornament
EP1783223A1 (en) 1998-10-05 2007-05-09 Novozymes A/S Fungal transcriptional activator PRTT from Aspergillus niger, useful in methods for producing polypeptides
US6806062B1 (en) * 1998-10-05 2004-10-19 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
JP4741082B2 (ja) 1999-02-22 2011-08-03 ノボザイムス アクティーゼルスカブ オキサロ酢酸ヒドロラーゼ欠陥真菌宿主細胞
CN100482801C (zh) 1999-03-22 2009-04-29 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
ATE469223T1 (de) 2000-03-14 2010-06-15 Novozymes As Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
KR20020026456A (ko) * 2000-04-13 2002-04-10 에말파브 마크 아론 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114736880A (zh) * 2022-06-09 2022-07-12 中国农业科学院北京畜牧兽医研究所 酸稳定性提高葡萄糖氧化酶GoxM10的突变体D497N及其衍生突变体和应用

Also Published As

Publication number Publication date
US7771971B2 (en) 2010-08-10
US20100323448A1 (en) 2010-12-23
CN100547078C (zh) 2009-10-07
JP4638418B2 (ja) 2011-02-23
US8445283B2 (en) 2013-05-21
US7303877B2 (en) 2007-12-04
EP1613761A2 (en) 2006-01-11
DE602004022929D1 (de) 2009-10-15
DK1613761T3 (da) 2009-12-14
US20080044858A1 (en) 2008-02-21
US20040191864A1 (en) 2004-09-30
EP1613761B1 (en) 2009-09-02
CN1798848A (zh) 2006-07-05
WO2004090155A3 (en) 2005-01-06
EP1613761A4 (en) 2006-08-09
JP2006521826A (ja) 2006-09-28
ATE441664T1 (de) 2009-09-15
WO2004090155A2 (en) 2004-10-21

Similar Documents

Publication Publication Date Title
CN101629204A (zh) 在缺乏酶的黑曲霉突变体中生产生物物质的方法
US9790532B2 (en) Methods for producing polypeptides in enzyme-deficient mutants of Fusarium venentatum
EP2588616B1 (en) A method for the production of a compound of interest
RU2608639C2 (ru) Мицелиальные грибы, имеющие фенотип измененной вязкости
EP4055177B1 (en) Fungal strains comprising enhanced protein productivity phenotypes and methods thereof
EP3775222B1 (en) Fungal chaperone proteins
CN115427434A (zh) 用于增强丝状真菌细胞中的蛋白质产生的组合物和方法
WO2014100612A1 (en) Methods for producing heterologous polypeptides in mutants of trichoderma

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20100120