CN101613761B - SSR markers lined with major gene of cotton fiber strength - Google Patents
SSR markers lined with major gene of cotton fiber strength Download PDFInfo
- Publication number
- CN101613761B CN101613761B CN 200910162731 CN200910162731A CN101613761B CN 101613761 B CN101613761 B CN 101613761B CN 200910162731 CN200910162731 CN 200910162731 CN 200910162731 A CN200910162731 A CN 200910162731A CN 101613761 B CN101613761 B CN 101613761B
- Authority
- CN
- China
- Prior art keywords
- cotton
- chain
- cotton fiber
- fiber strength
- labeled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloser SSR markers lined with major genes of cotton fiber strength, which is obtained by the following steps: generating F2 and F2:3 populations by using a cotton search 41 line sGK9708 selected from cultivated varieties of gossypium hirsutum and a high quality line 0-153 of gossypium hirsutum as parents; allowing generation within the family of the F2:3 population to self cross till the F2:6 generation, performing within-family individual selection of the F2:6 generation once, and planting two generation till F6:8; performing polymorphism screening of the parents by using SSR primers and creating an RIL population linkage map; and performing the multi-environment major QTL screening of the cotton fiber strength to screen 6 QTLs of a cotton fiber strength character from line 0-153, wherein 5 QTLs are multi-environment stable QTLs and are FS1 linkage marker NAU2119330, FS2 linkage markers BNL2572125, BNL1064110 and DPL0874210, FS4 linkage markers are NAU1048250 and NAU2627350, and FS5 linkage markers BNL1421200 and NAU2730450. The SSR markers lined with the major genes of the cotton fiber strength are screen from high fiber quality materials and used as molecular markers to perform early auxiliary selection on a DNA level to improve the selection efficiency of the cotton fiber strength.
Description
Technical field
The present invention relates to from excellent fibrous quality germplasm materials 0-153 and chain SSR (the Simple Sequence Repeats of major gene of high-strength cotton fibre; Simple sequence repeats) molecule marker; These marks are to obtain with colony's molecule marker genetic map binding analysis through the performance of the fibrous quality under many environment; Utilize these and the closely linked molecule marker of fibre strength to carry out assisted Selection and can improve the cotton fiber quality breeding efficiency, belong to biological technology application.
Background technology
Cotton fibre is the of paramount importance economic characters of cotton, also is important textile industry raw material.Along with the fast development of textile industry and improving constantly of living standards of the people, also increasingly high to the requirement of cotton fiber quality.Cotton in China breeding work person mainly concentrates on before the eighties in last century in the raising of output, and the raising of fibrous quality does not obtain enough attention, makes the cotton variety of China show as output height, poor quality mostly.Yang Wei China etc. draw the analysis of the fibrous quality of China's self-fertile cotton variety over nearly 20 years; The modal staple length or 2.5% length of span of China's self-fertile upland cotton kind mainly concentrate on 27～30mm; The fracture specific tenacity concentrates on 17～23cN/tex, and mic value concentrates on 3.7～4.9.Compare with the U.S., have superiority on the staple length, but the fiber specific tenacity slightly be inferior to the U.S. (Yang Wei China, Xiang Shikang .2001 such as Tang Shurong, China's self-fertile cotton variety fibrous quality analysis over 20 years. cotton journal .13 (6): 377-384).The 2001-2005 Ministry of Agriculture shows the main sampling result of planting the cotton variety fiber of the cotton province of China's main product: staple length mainly is distributed in 28-29mm, and specific tenacity is in medium level on the upper side, mainly is distributed in 27-29cN/tex; Present Cotton in China fiber quality can satisfy in the textile industry, requirement (Tang Shurong, Meng Junting, Chu's equality of low-grade cotton yarn; 2007; Cotton in China fibrous quality As-Is analysis, Jiangxi agricultural journal, 19 (7): 8-10).Though fibrous quality such as staple length and specific tenacity proterties increases, but the kind of high-count yarn spun still lacks.Over the past two years, authorized some length more than 31mm, the upland cotton kind of specific tenacity more than 30cN/tex, but quality is single, and the coupling between the cotton fibre quality index is also undesirable, and the fiber specific tenacity of most of upland cotton Cultivar is still on the low side.Therefore, need to quicken to improve fibrous quality, especially the fiber specific tenacity of Cotton in China kind.
Adopt traditional breeding method to improve the fiber specific tenacity of upland cotton; At first to pass through upland cotton and hybridization such as sea island cotton or Asiatic cotton; Backcross through polybasic, obtain to have the germplasm materials that the high-intensity fiber gene gradually oozes, with existing upland cotton Cultivar hybridization; Backcross and the selfing selection through polybasic, to break the negative correlation between fibrous quality proterties and yield traits.Traditional breeding method mainly is according to Phenotypic Selection; Need enough big selection colony, each selects all will measure fibrous quality from generation to generation, and cost is higher and workload is big; And the fibrous quality proterties is affected by environment bigger, makes the fibrous quality breeding make slow progress.
Molecular marking technique can carry out a large amount of individualities or family in the short period of time and detect; Because the genotype of molecule marker can be discerned; If objective trait and certain molecule marker close linkage; Just can utilize molecule marker that objective trait is selected, can improve the efficient that detects and select greatly.At present molecular marker screening has been done a large amount of work both at home and abroad, utilized different colonies to carry out the structure of linkage map and the QTL screening of important fibrous quality proterties, obtained a large amount of QTLs relevant with fibrous quality.Utilizing F
2And BC
1On the isolates body, (2000) such as (1998), Ulloa such as Shappley etc. (1998), Jiang utilize the RFLP mark to detect more than 40 significance QTLs relevant with fibrous quality altogether; (2003) such as Yuan Youlu etc. (2000,2001) and Zhang utilize a unusual cotton high-intensity fiber gradually to ooze to be 7235 with upland cotton heredity standard be that TM-1 has made up F for the parent
2, F
2:3Segregating population has been identified a fibre strength main effect QTL, soluble phenotypic variation more than 30%; Kohel etc. (2001) utilize F between the kind of extra large land
2Colony has identified 13 QTLss relevant with the fibrous quality proterties, and the phenotypic variation of explaining single proterties by these QTLs wherein has the QTLs of 4 fibre strength proterties between 30-60%; Paterson etc. (2003) utilize F between a kind
2:3Colony has obtained 68 QTLss relevant with the fibrous quality proterties, and wherein the fibre strength proterties is 21; Wu etc. (2003) utilize sea, land hybrid F
2Colony detects 2 intensity Q TLs, 13.86% and 34.15% of interpret table form variation; Mei (2004) utilizes F between the kind of extra large land
2Colony and RFLP, AFLP and three kinds of marks of SSR have been located the QTLs of 5 fibrous qualities, all explain bigger phenotypic variation.Shen Xinlian etc. (2004) utilize the composite interval mapping method to screen 38 QTLss relevant with fibrous quality altogether, and wherein 10 is fibre strength proterties QTLs; Lacape etc. (2005) utilize backcross population to screen 80 QTLs of 11 fibrous quality proterties, and wherein 12 QTLs of fibre strength can both detect in one or more generations.Lin etc. (2005) utilize sea, land F
2Colony adopts SRAP, SSR, three kinds of marker detection to 13 of RAPD QTLs relevant with fibrous quality, and wherein fibre strength is 2, can explain 21.18%～21.66% phenotypic variation; Zhang etc. (2005) utilize F in the cotton seed of land
2And F
2:3Colony has identified 12 QTLs that the fibrous quality proterties is relevant, and wherein fibre strength is 3; He etc. (2007) adopt SSR, SRAP, RAPD, four kinds of differences of REMAP to be marked at sea, land F
2:3Find the QTLs of 16 fibrous quality proterties in the colony, wherein 3 of fibre strength, can explain the phenotypic variation of 15.26-37.12%; The QTL of 20 fibrous quality proterties has located in the F2 colony that Qin etc. (2008) utilize four upland cotton parents to make up; Wherein 9 is significance QTL; 11 QTL for existing, the 5.1%-25.8% of interpret table form variation has 5 to be the QTLs of fibre strength proterties altogether.
Utilize in the RIL colony, James etc. (2006) have detected 25 QTLs relevant with cotton fiber quality, and wherein 6 of controlling fiber intensity, the phenotypic variation of soluble 5.4-6.5%; Wang etc. (2006) utilize the SSR marker detection to 48 QTLs, the wherein QTLs of 4 fibre strength proterties; Shen etc. (2007) utilize the relevant QTLs of RIL mass screening to 27 fibrous quality in the cotton seed of land, and wherein the fibre strength proterties is 5, and 1 ability is expressed under different environment, 10.52% of ability interpret table form variation; Wu etc. (2009) utilize RIL colony in the cotton seed of land, locate the QTL of 34 fibrous quality proterties altogether, wherein 6 fibre strength QTLs; Zhang etc. (2009) utilize the RIL colony in the cotton seed of land to detect the QTL of 13 fibrous quality proterties, and wherein 2 fibre strength QTLs can detect 10.2%～31.8% of interpret table form variation under two environment.
These results of study are that the molecular marker assisted selection of fibrous quality proterties is laid a good foundation.But; More in the former study is to utilize segregating population; Or only under single environment, detect the result who obtains, therefore lack reliability and stability, and the initial purpose of some research is in order to carry out the location of target gene; In the selection of experiment material, do not have consideration and breeding material to combine, also be difficult to be applied in the breeding.
Summary of the invention
The present invention is through screening from SSR chain with major gene of high-strength cotton fibre in the excellent fibrous quality material (Simple Sequence Repeats; Simple sequence repeats) molecule marker, utilize these and the closely linked molecule marker of fibre strength to carry out the efficiency of selection that early stage assisted Selection on the dna level can improve cotton fiber strength.
Technical scheme provided by the invention is: a kind of acquisition and the chain molecular marker method of cotton fiber strength major gene loci is characterized in that this method comprises the steps:
1) upland cotton Cultivar middle cotton institute 41 choosings that utilize the land for growing field crops to promote are sGK9708 and have the upland cotton high-quality strain 0-153 that Asiatic cotton high-intensity fiber gene gradually oozes and make up F for the parent
2, F
2:3Colony;
2) F
2:3Per generation selfing in colony's family is until F
2:6In generation, is at F
2:6In generation, carried out an interior individual plant selection of family, purifies in order to family, planted for two generations again to F
6:8
3) select for use 5742 pairs of SSR primers having announced to carry out between the parent polymorphum screening, the molecule marker results of preliminary screening shows that 191 pairs of SSR primers are arranged is variant between the parent, with polymorphism primer to 196 F
6:8Family colony increases, and has obtained 217 pleomorphism sites, makes up RIL colony linkage map;
4) the fibre strength main effect QTL that carries out under many environment screens; Filter out 6 QTLs altogether from the cotton fiber strength proterties of 0-153; Wherein 5 is the QTLs of many ambient stables; That is: be positioned at FS1, FS2 on the karyomit(e) c25, be positioned at karyomit(e) c7 and go up FS3, FS4, be positioned at the FS5 on the karyomit(e) c13; Mark NAU2119
330Chain with FS1; Mark BNL2572
125, BNL1064
110, DPL0874
210Chain with FS2; Mark NAU1048
250, NAU2627
350Chain with FS4; Mark BNL1421
200, NAU2730
450Chain with FS5.
The above-mentioned the 3rd) step selects for use 5742 pairs of SSR primers having announced to carry out polymorphum screening between the parent, and the PCR reaction system is 10 μ l, wherein ultrapure water 6.40 μ l; 10 * Buffer, 1.0 μ l, 10mM dNTPs 0.50 μ l, 10 μ M forward primers, 0.50 μ l; 10 μ M reverse primers, 0.50 μ l; 30ng/ μ l template DNA 1.0 μ l, 5U/ μ lTaq archaeal dna polymerase 0.10 μ l, the PCR response procedures is: 94 ℃ of preparatory sex change 45s; 94 ℃ of sex change 30s, 57 ℃ of annealing 45s, 72 ℃ are extended 1min, 29 circulations; 94 ℃ of sex change 60s, 57 ℃ of annealing 45s, 72 ℃ are extended 2min, and PCR is reflected on TGRADIENT and the PTC-200 and carries out, and amplified production carries out electrophoresis in 8% polyacrylate hydrogel, the record result.
Of the present invention have a following beneficial effect:
4 sites (FS1, FS2, FS4, FS5) relevant involved in the present invention with the cotton fiber strength major gene; The synergy gene is all from excellent fiber parent material 0-153; And effect is stable in a plurality of environment that detected, and can be used for the molecular marker assisted selection of cotton fiber strength.FS1 interpret table form variation under four environment is all bigger, is respectively 19.12%, 14.25%, 15.38%, 18.43%, and additive effect is also bigger, is respectively 1.0510,0.8067,0.9823,1.1026cN/tex; FS2 interpret table form variation under the border, Fourth Ring is also bigger, is respectively 21.36%, 18.05%, 14.69%, 17.47%, and additive effect is also bigger, is respectively 1.1216,0.9083,0.9646,1.0785cN/tex; FS4 is under four environment respectively 2.79%, 4.87%, 6.54%, 5.66% of the interpret table form variation, and additive effect is respectively 0.4037,0.4737,0.6341,0.5982cN/tex; FS5 detects under two environment, and respectively 6.87%, 3.80% of the interpret table form variation, additive effect is respectively 0.5338,0.4741cN/tex.
Description of drawings
Fig. 1 is RIL of the present invention colony part molecular marker linkage maps.
Wherein, FS1 is positioned at BNL3806 between the mark zone of karyomit(e) c25
190-CM067
145In, be attached thereto the BNL3806 that is labeled as of lock
190, TMK19
180, BNL3806
230, NAU1054
220, N AU2119
330, NAU1054
320, CM067
145FS2 is positioned at BNL2572 between the mark zone of karyomit(e) c25
125-BNL1440
240In, think the chain BNL2572 that is labeled as with it
125, JESPR215
140, BNL1064
110, DPL0874
210, BNL1440
240FS3 is positioned at NAU1085 between the mark zone of karyomit(e) c7
260-TMB1618
180In, be attached thereto the NAU1085 that is labeled as of lock
260, TMB1618
180FS4 is positioned at NAU1048 between the mark zone
230-NAU2627
350In, be attached thereto the NAU1048 that is labeled as of lock
250, NAU2627
350FS5 is positioned at BNL1421 between the mark zone
200-NAU2730
450, the chain with it BNL1421 that is labeled as
200, NAU2730
450
Embodiment
Come further to illustrate the present invention through the detailed description of embodiment below, the molecule marker chain with major gene of high-strength cotton fibre draws through following method:
Upland cotton kind middle cotton institute 41 choosings of (1) cultivating with The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute are that sGK9708 is a female parent; Sichuan Agricultural University's seed selection to have the high-intensity fiber germplasm line 0-153 that Asiatic cotton gradually oozes be male parent; Calendar year 2001 is experimental field disposed cross combination in The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute, plantation F in 2002
1, selfing produces F
2Experimental field planted F in The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute in 2003
2, plant parent and F simultaneously
1Each two row is gathered in the crops 250 individual plants, with all individual plant selfing, obtains F
2:3Seed.Divide individual plant to receive flower, and take by weighing the 12-15g fiber sample and carry out fibrous quality and measure.Choose 196 individual plant kinds in 2004 and become F
2:3Plant, every family selfing obtains F
2:4Seed, per stirpes is received flower, and chooses the 12-15g fiber sample and carry out fibrous quality mensuration.Winter in 2004, the adding generation in Hainan, plantation F
2:4Selfing obtains F in the seed, family
2:5Seed.2005 in the plantation F of The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute
2:5Seed, every family selfing obtains F
2:6Seed.Winter in 2005 was planted F in Hainan
2:6Family is carried out an individual plant and is selected, and carries out purifying in the family.2006 in the plantation F of The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute
6:7Seed, results F
6:8For seed.2007 in the plantation F of The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute
6:8Seed, and adopt incomplete randomized block design, two repetitions are set, results F
6:9Seed, the later stage per stirpes is received flower, gets the mensuration that the 12-15g fiber sample is used for fibrous quality.2008 in The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute, testing station, Shandong Cotton Research Center Linqing, the Hebei Quzhou experiment centre plantation F of China Agricultural University
6:9Seed adopts incomplete randomized block design, and plantation two repeats, and per stirpes is received flower, gets the mensuration that the 12-15g fiber sample carries out fibrous quality.
(2) get F
6:8For the blade of RIL colony, adopt the CTAB method to extract 196 familys and two parents' DNA.
(3) adopt the SSR mark to carry out the screening of high-intensity fiber mark; At first select for use 5742 pairs of SSR primers that the polymorphum between two parents is screened; In these SSR primers; Wherein the primer with BNL numbering is Brookhaven National Laboratory (U.S.) exploitation, with the NAU numbering be Agricultural University Of Nanjing (China) exploitation, the CIR numbering be international farming research centre of development (France) exploitation; With CM and JESPR numbering is the agro-industrial university in Dezhou (U.S.) exploitation; With TMB and MGHES numbering be the exploitation of farming research service administration of USDA, MUCS and MUSS numbering be that University of California Davis (U.S.) develops etc., what DPL numbered is that Delta and Pine Land company (U.S.) develops.Primer gives birth to the worker by Shanghai and Bioasia company is synthetic.The SSR amplification reaction system is 10 μ l, ultrapure water 6.40 μ l wherein, 10 * Buffer, 1.0 μ l; 10mM dNTPs 0.50 μ l, forward primer (10 μ M) 0.50 μ l, reverse primer (10 μ M) 0.50 μ l; Template DNA (30ng/ μ l) 1.0 μ l, Taq archaeal dna polymerase (5U/ μ l) 0.10 μ l.SSR amplified reaction program: 94 ℃ of preparatory sex change 45s; 94 ℃ of sex change 30s, 57 ℃ of annealing 45s, 72 ℃ are extended 1min, 29 circulations.94 ℃ of sex change 60s, 57 ℃ of annealing 45s, 72 ℃ are extended 2min.Amplified reaction carries out on BIOMETRA company's T GRADIENT and the PTC-200 of BIO-RAD company, and amplified production carries out electrophoresis in 8% polyacrylate hydrogel, carries out gel silver according to the method for Zhang Jun (2000) and dyes, the record result.Molecular marker screening is the result show, has 191 pairs of SSR primers between the parent, to show polymorphum.
(4) select 191 pairs of primers that polymorphum is arranged for use between the parent, to the analysis of increasing of 196 RIL family colonies, SSR reaction system and SSR amplified reaction program and amplified reaction, gel electrophoresis is all accomplished with reference to (3), has obtained 217 pleomorphism sites altogether.
(5) utilize Joinmap3.0 software building genetic linkage maps; The Kosambi function is adopted in mapping, sets LOD value 7.0, has made up and has contained 190 sites; 34 linkage groups; Covering gene group 700.9cM accounts for the RIL colony molecule linkage map of genome 15.75%, and 25 linkage groups have navigated on the karyomit(e).
(6) the mensuration result of combination RIL colony fibrous quality; Utilize Windows QTL Cartographer2.5 to carry out the QTLs mapping, setting the LOD value is 2.5, carries out the test of 1000 minor sorts; The fibre strength main effect QTL of stably express under screening and the many environment; Screen the QTLs of 6 fibre strength proterties, wherein 5 is the main effect QTL s of many ambient stables, lays respectively on karyomit(e) c25 (2), c7 (2) and the c13.FS1, FS2 are positioned on the karyomit(e) c25, and FS3, FS4 are positioned on the karyomit(e) c7, and FS5 is positioned on the karyomit(e) c13.With FS1 chain be labeled as NAU2119
330, near other mark BNL3806
190, TMK19
180, BNL3806
230, NAU1054
220, NAU1054
320CM67
145In the research of Shen (2005), Lacape (2005), Shen (2007) and this laboratory Zhang Jianhong (2007), report; With FS2 chain be labeled as BNL2572
125, BNL1064
110, DPL0874
210, near other mark JESPR215
140, BNL1440
240In the research of Zhang Jianhong (2007), report; With FS4 chain be labeled as NAU1048
250, NAU2627
350With FS5 chain be labeled as BNL1421
200, NAU2730
450Have high-intensity fiber germplasm 0-153 that Asiatic cotton gradually oozes and middle cotton 41 choosings be in the RIL colony that makes up of sGK9708 hybridization; FS1 is under four environment respectively 19.12%, 14.25%, 15.38%, 18.43% of the interpret table form variation, and additive effect is respectively 1.0510,0.8067,0.9823,1.1026cN/tex; FS2 is under the border, Fourth Ring respectively 21.36%, 18.05%, 14.69%, 17.47% of the interpret table form variation, and additive effect is respectively 1.1216,0.9083,0.9646,1.0785cN/tex; FS4 is under four environment respectively 2.79%, 4.87%, 6.54%, 5.66% of the interpret table form variation, and additive effect is respectively 0.4037,0.4737,0.6341,0.5982cN/tex; FS5 detects under two environment, and respectively 6.87%, 3.80% of the interpret table form variation, additive effect is respectively 0.5338,0.4741cN/tex.
In addition, we have made up a cover upland cotton double cross F
2Colony; Utilize with linked mark TMK19 and the CM067 of FS1 the fibre strength proterties is assisted pyramiding breeding research (Dong Zhanghui; 2008); Find that polymerization has the difference of the material fiber intensity of this QTL to reach significantly or utmost point significant difference, explain and utilize the marker assisted selection of carrying out the fibrous quality proterties with the linked mark of these QTL, can improve the fibrous quality of upland cotton greatly.
Claims (3)
1. with cotton fiber strength major gene loci FS1, FS2, FS4, molecule marker that FS5 is relevant; It is characterized in that: FS1, FS2, FS4, the FS5 position on karyomit(e) is as shown in Figure 1; FS1, FS2 are positioned on the karyomit(e) c25; FS4 is positioned on the karyomit(e) c7, and FS5 is positioned on the karyomit(e) c13, with FS1 chain be labeled as NAU2119
330With FS2 chain be labeled as BNL2572
125, BNL1064
110, DPL0874
210With FS4 chain be labeled as NAU1048
250, NAU2627
350With FS5 chain be labeled as BNL1421
200, NAU2730
450
2. according to claim 1 and cotton fiber strength major gene loci FS1, FS2, FS4, the molecule marker that FS5 is relevant is characterized in that obtaining through following method:
1) upland cotton Cultivar middle cotton institute 41 choosings that utilize the land for growing field crops to promote are sGK9708 and have the upland cotton high-quality strain 0-153 that Asiatic cotton high-intensity fiber gene gradually oozes and make up F for the parent
2, F
2:3Colony;
2) F
2:3Per generation selfing in colony's family is until F
2:6In generation, is at F
2:6In generation, carried out an interior individual plant selection of family, purifies in order to family, planted for two generations again to F
6:8
3) select for use 5742 pairs of SSR primers having announced to carry out between the parent polymorphum screening, the molecule marker results of preliminary screening shows that 191 pairs of SSR primers are arranged is variant between the parent, with polymorphism primer to 196 F
6:8Family colony increases, and has obtained 217 pleomorphism sites, makes up RIL colony linkage map;
4) the fibre strength main effect QTL that carries out under many environment screens; Filter out 6 QTLs altogether from the cotton fiber strength proterties of 0-153; Wherein 5 is the QTLs of many ambient stables, that is: FS1, FS2 are positioned on the karyomit(e) c25, and FS4 is positioned on the karyomit(e) c7; FS5 is positioned on the karyomit(e) c13, with FS1 chain be labeled as NAU2119
330With FS2 chain be labeled as BNL2572
125, BNL1064
110, DPL0874
210With FS4 chain be labeled as NAU1048
250, NAU2627
350With FS5 chain be labeled as BNL1421
200, NAU2730
450
3. according to claim 2 and cotton fiber strength major gene loci FS1, FS2, FS4, the molecule marker that FS5 is relevant, it is characterized in that: the 3rd) step selects for use 5742 pairs of SSR primers having announced to carry out polymorphum screening between the parent, and the PCR reaction system is 10 μ 1; Ultrapure water 6.40 μ 1 wherein; 10 * Buffer, 1.0 μ 1,10mM dNTPs 0.50 μ 1,10 μ M forward primer 0.50 μ 1; 10 μ M reverse primers, 0.50 μ 1; 30ng/ μ 1 template DNA 1.0 μ 1,5U/ μ 1Taq archaeal dna polymerase 0.10 μ 1, the PCR response procedures is: 94 ℃ of preparatory sex change 45s; 94 ℃ of sex change 30s, 57 ℃ of annealing 45s, 72 ℃ are extended 1min, 29 circulations; 94 ℃ of sex change 60s, 57 ℃ of annealing 45s, 72 ℃ are extended 2min, and PCR is reflected on TGRADIENT and the PTC-200 and carries out, and amplified production carries out electrophoresis in 8% polyacrylate hydrogel, the record result.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910162731 CN101613761B (en) | 2009-08-12 | 2009-08-12 | SSR markers lined with major gene of cotton fiber strength |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910162731 CN101613761B (en) | 2009-08-12 | 2009-08-12 | SSR markers lined with major gene of cotton fiber strength |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101613761A CN101613761A (en) | 2009-12-30 |
| CN101613761B true CN101613761B (en) | 2012-11-28 |
Family
ID=41493646
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910162731 Expired - Fee Related CN101613761B (en) | 2009-08-12 | 2009-08-12 | SSR markers lined with major gene of cotton fiber strength |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101613761B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101880714B (en) * | 2010-06-04 | 2012-08-22 | 华中农业大学 | Method for building cotton fiber transcription genetic linkage map by EST-SSR sign |
| CN102321619B (en) * | 2011-01-28 | 2012-12-19 | 中国农业科学院棉花研究所 | Molecular marker linked with major QTL (quantitative trait loci) of brown cotton fiber colors and application thereof |
| CN102220318B (en) * | 2011-05-10 | 2013-08-28 | 中国农业科学院棉花研究所 | SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect genes |
| CN102181442B (en) * | 2011-05-10 | 2013-07-10 | 中国农业科学院棉花研究所 | Molecular label relevant with cotton fiber strength and arising from high-quality variety Xinluzao No.24 |
| CN104404153A (en) * | 2014-12-03 | 2015-03-11 | 西南大学 | Molecular markers interlocked with fiber quality trait genes of chromosome 6 of upland cotton |
| CN107201403B (en) * | 2017-06-10 | 2020-09-04 | 南通大学 | Cotton fiber length related QTL and application thereof |
| CN110093345B (en) * | 2019-05-09 | 2020-11-10 | 江苏省农业科学院 | SSR molecular marker related to cotton fiber strength and micronaire value and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1293256A (en) * | 2000-11-10 | 2001-05-02 | 南京农业大学 | Molecular label linked with major gene of high-strength cotton fibre |
| CN1528912A (en) * | 2003-10-21 | 2004-09-15 | 南京农业大学 | Cotton high-strong fiber gene major gene site and moloecular labelling thereof |
-
2009
- 2009-08-12 CN CN 200910162731 patent/CN101613761B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1293256A (en) * | 2000-11-10 | 2001-05-02 | 南京农业大学 | Molecular label linked with major gene of high-strength cotton fibre |
| CN1528912A (en) * | 2003-10-21 | 2004-09-15 | 南京农业大学 | Cotton high-strong fiber gene major gene site and moloecular labelling thereof |
Non-Patent Citations (1)
| Title |
|---|
| TZ Zhang et al.Marker Name：NAU2119.《Cotton Marker Database》.2005, * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101613761A (en) | 2009-12-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101613761B (en) | SSR markers lined with major gene of cotton fiber strength | |
| CN101138313B (en) | Maize inbred line resistant to MRDV bred by using molecule making | |
| CN102181442B (en) | Molecular label relevant with cotton fiber strength and arising from high-quality variety Xinluzao No.24 | |
| CN103497949B (en) | The molecule marker relevant with cotton fiber strength from sea island cotton sea 1 and application thereof | |
| CN102229979B (en) | Molecular breeding method capable of improving length of cotton fibers by using gossypium barbadense chromosome segment introgression line | |
| CN104313016B (en) | The molecular labeling of the QTL/ major gene resistance relevant with cotton verticillium wilt resistance | |
| CN103571852A (en) | Sea island cotton chromosome segment capable of obviously increasing upland cotton ginning outturn and SSR (simple sequence repeat) marker of sea island cotton chromosome segment | |
| CN113179945A (en) | Breeding method of high-yield lodging-resistant disease-resistant new wheat variety | |
| CN105524999A (en) | Molecular marker linked with sea island cotton fiber length of cotton | |
| CN104313155A (en) | Floral character associated molecular marker screening method of amenone form chrysanthemum and application of method | |
| CN105483248B (en) | From sea island cotton molecular labeling related with fibre strength and its application | |
| CN105200053B (en) | From extra large 1 molecular labeling related with fibre length of sea island cotton and its application | |
| CN102220318B (en) | SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect genes | |
| CN103114145B (en) | Soybean mosaic virus major gene locus and application thereof | |
| CN102229982B (en) | Molecular breeding method for improving cotton fiber length, fiber strength, and micronaire value | |
| CN114032235B (en) | SSR marker, primer pair, application of primer pair and screening method of SSR marker locus related to upland cotton early-maturing molecular breeding | |
| CN108130380B (en) | Molecular breeding method for synchronously improving verticillium wilt resistance and fiber quality of cotton | |
| CN105524998A (en) | Molecular marker linked with sea island cotton fiber strength of cotton | |
| CN102517396B (en) | Molecular breeding method for breeding new anti-verticillium cotton seed | |
| CN102229981B (en) | Molecular breeding method for improving strength and micronaire value of cotton fibers | |
| CN102229980B (en) | Molecular breeding method for improving micronaire value of cotton by using chromosome segment introgression lines of island cotton | |
| CN103602742A (en) | Molecular marking method for identifying variety of Suzhi No.1 hybrid zoysia | |
| CN105177175B (en) | From extra large 1 molecular labeling related with fiber mic value of sea island cotton and its application | |
| CN107980618A (en) | A kind of molecular breeding method using the mono- QTL fragments substitution line improvement cotton fiber strengths of chr.7 | |
| CN108130381A (en) | From extra large 1 molecular labeling related with resistance to verticillium wilt of sea island cotton and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121128 Termination date: 20160812 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |