CN104404153A - Molecular markers interlocked with fiber quality trait genes of chromosome 6 of upland cotton - Google Patents
Molecular markers interlocked with fiber quality trait genes of chromosome 6 of upland cotton Download PDFInfo
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- CN104404153A CN104404153A CN201410723250.8A CN201410723250A CN104404153A CN 104404153 A CN104404153 A CN 104404153A CN 201410723250 A CN201410723250 A CN 201410723250A CN 104404153 A CN104404153 A CN 104404153A
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Abstract
The invention discloses molecular markers interlocked with fiber quality trait genes of chromosome 6 of upland cotton. Markers interlocked with qFL, qFS and qFM are PGML3033, NBRI0277, SWU1781, SWU2422, SWU2282, SWU2454, SWU2436, T1, SWU1799, SRAP37F27R, SWU2138, MUSB0103, MUSS122, CGR5355, NAU1218, SWU1794, SWU2098, NAU0874 and SWU06-077. According to the molecular marker disclosed by the invention, the markers interlocked with the fiber quality genes are used for assistant selection, so that the efficiency of quality breeding of upland cotton fibers can be greatly improved.
Description
Technical field
The present invention relates to the molecule marker with the gene linkage of upland cotton the 6th chromosome fibre quality trait, belong to biological technology application.
Background technology
Cotton is natural fiber crop primary in the world, and cotton fibre is important textile industry raw material.Gossypium comprises 45 diploid kinds and 5 Tetraploids (Percival etc. 1999), wherein has 4 cultivars.Cultivar has two two times of kinds (cotton and Asiatic cotton) and two Tetraploids (upland cotton and sea island cotton), and wherein upland cotton produces the raw cotton (Chen etc. 2007) in the whole world more than 95%.China produces gined cotton about 6,000,000 tons per year, and needs raw cotton greatly about about 6,500,000 tons in textile industry year.The fibrous quality that cotton variety is now educated by China can meet the requirement of current textile industry substantially, but staple length is single, low strength, fineness is thicker, lack the kind spinning more than 40 cotton yarns, especially lack the kind (Xiang Shikang etc., 1999) spinning more than 60 high-grade cotton yarns.Along with the raising of the updating of textile technology, living standards of the people, and the aggravation of the international raw cotton market competition of Post-WTO, how to improve fibrous quality as early as possible, cultivate the concern that good quality and high output kind is more and more subject to vast cotton breeding worker.
The proterties such as output of cotton and fibrous quality belongs to the quantitative character of controlled by multiple genes, easily affected by environment.The methods such as traditional cotton flower variety genetic improvement mainly adopts distant hybirdization, backcrosses, interbreed, mutually friendship, these methods serve vital role in the cotton variety seed selection of 20th century.But because traditional breeding way adopts Phenotypic Selection, cannot select the gene controlling proterties, therefore breeding cycle is longer, and efficiency is low.
The improvement quantitative character that develops into of modern molecular mark provides one method (Tanksley and Hewitt, 1988) fast and effectively.Quantitative trait locus (the quantitative trait locus such as utilization and yield and quality, QTL) closely linked molecule marker, molecular marker assisted selection is carried out to proterties such as yield and qualities, greatly can improve efficiency of selection, accelerate breeding process.Since (1994) reports such as Reinisch first cotton RFLP (restriction fragment length polymorphism) linkage map, utilize upland cotton and sea island cotton species hybridization colony, the genetic linkage maps relative saturation (Jiang etc. 1998 built, Zhang etc. 2002, Lacape etc. 2003, Nguyen etc. 2004, Rong etc. 2004, Guo etc. 2007, 2008, He etc. 2007, Yu etc. 2007, Zhang etc. 2008), and utilize Upland Cotton intermolecular hybrid colony, the genetic linkage maps genome fraction of coverage about 40% that the builds (1998a such as Shappley, b, Ulloa and Meredith2000, Ulloa etc. 2002, Shen etc. 2005, 2007, Zhang etc. 2005, Wang Juan etc. 2007, Chen Li etc. 2008, Zheng Jing etc. 2008, Qin etc. 2008).Utilize upland cotton and sea island cotton species hybridization population genetic linkage map (Jiang etc. 1998, Yu etc. 1998, Paterson etc. 2003, Kohel etc. 2001, Mei etc. 2004, the 2005a such as Chee, b, Draye etc. 2005, Lacape etc. 2005, Lin etc. 2005, He etc. 2007), and the Upland Cotton intermolecular hybrid population genetic linkage map (1998b such as Shappley, Ulloa and Meredith2000, Zhang etc. 2005, Shen etc. 2005, 2007, Wan etc. 2007, Wang Juan etc. 2007, Chen Li etc. 2008, Qin etc. 2008), assignment of genes gene mapping research has been carried out to the proterties such as output of cotton and fibrous quality, obtain a large amount of QTL at present.In located gene/QTL, except the cotton cytoplasmic sterility Restore gene that (2006) such as Yin locate, proterties QTL such as other output and fibrous quality etc. and its linked marker, apart from remote, can't meet the requirement of molecular marker assisted selection and map based cloning.Cause the major cause of this result: one is that upland cotton and sea island cotton species hybridization colony do not set up QTL Fine Mapping colony, individuality (individual plant/strain) included by existing colony is less, can only meet the structure of frame diagram and the requirement of QTL Primary Location; Two is that molecule marker polymorphism between Upland Cotton is low, does not have abundant molecule marker to increase collection of illustrative plates mark density, even if establish QTL Fine Mapping colony, and the also difficult object reaching Fine Mapping.
The present invention is directed at present locate the proterties QTL such as output, fibrous quality and its linked marker apart from remote, the difficult point of molecular marker assisted selection and map based cloning requirement can't be met; Utilize Upland Cotton intermolecular hybrid population genetic linkage map to there is molecule marker at minority chromosomal region intensive, there is the feature such as fibrous quality and ginning outturn QTL in the intensive chromosomal region of portion markings simultaneously; According to (cotton No. 1 of T586 × Chongqing) F
2:7the marker genetype of recombinant inbred strain colony of colony strain coloured differently body, select the 6th karyomit(e) from T586, and other karyomit(e) is the strain RIL118 of Chongqing cotton No. 1 background, hybridize with cotton No. 1 of Chongqing, set up (cotton No. 1 of the RIL118 × Chongqing) F comprising 6975 individual plants
2colony.On the basis of the 6th chromosome genetic linkage map building relative saturation, in conjunction with (cotton No. 1 of RIL118 × Chongqing) F
2with F
2:3the qualification result of colony's fiber quality characteristics, Fine Mapping cotton the 6th chromosome fibre quality trait QTL, for carrying out molecular marker assisted selection to the 6th chromosome fibre quality trait QTL further and map based cloning lays the foundation.
Summary of the invention
The object of the invention is to: by screening with the quality trait gene linkage of upland cotton good fiber quality and show stable molecule marker under multiple environment, these molecule markers are applied to the assisted Selection of cotton fiber quality, the seed selection process of Cotton in China good fiber quality kind can be accelerated.
Technical scheme provided by the invention is: with qFL, qFS and qFM chain be labeled as PGML3033, NBRI0277, SWU1781, SWU2422, SWU2282, SWU2454, SWU2436, T1, SWU1799, SRAP37F27R, SWU2138, MUSB0103, MUSS122, CGR5355, NAU1218, SWU1794, SWU2098, NAU0874 and SWU06-077, detected QTL and linked marker thereof are all positioned on upland cotton the 6th karyomit(e).
In the present invention, QTL name, with reference to (1997) naming rules in paddy rice such as McCouch, represents with the form of q+ proterties.
The screening method of the mark chain with qFL, qFS and qFM, comprises the steps:
(1) Fine Mapping colony is set up: according to (cotton No. 1 of T586 × Chongqing) F
2:7the marker genetype of recombinant inbred strain colony strain coloured differently body, the 6th karyomit(e) is selected to provide for T586, and other karyomit(e) (especially the 7th chromosomal brown fibre site, the 12nd chromosomal photon site designation of chromosome section) is for changing the cotton No. 1 strain RIL118 provided, change cotton No. 1 with upland cotton high-quality kind and hybridize, set up (cotton No. 1 of the RIL118 × Chongqing) F comprising 6975 strains
2colony;
(2) F is extracted
2the DNA of colony and parent;
(3) the 6th chromosome genetic linkage map is set up: utilize the molecule marker be positioned on the 6th karyomit(e), to (cotton No. 1 of RIL118 × Chongqing) F
2colony carries out marker genetype detection, builds the 6th chromosomal genetic linkage maps, utilizes the molecular marker screening colony parent of new synthesis simultaneously, detects (cotton No. 1 of RIL118 × Chongqing) F
2colony's marker genetype, increases by the 6th chromosomal marker density;
(4) Fine Mapping the 6th chromosome fibre quality and ginning outturn QTL: utilize the 6th chromosome genetic linkage map built, in conjunction with (cotton No. 1 of RIL118 × Chongqing) F
2, F
2:3the qualification result of the fiber quality characteristics of colony, Fine Mapping the 6th chromosome fibre length, fibre strength, fiber mic value QTL, obtain 3 fibrous quality QTL on the upland cotton described in claim 1 the 6th karyomit(e) and linked marker thereof altogether.
3 the fiber quality characteristics gene locuss that the present invention relates to are qFL, qFS and qFM, and wherein, the effect-increasing loci of qFL and qFS derives from parent and changes cotton No. 1, and the effect-increasing loci of qFM derives from RIL118.By screening and these 3 closely linked molecule markers of QTL, these molecule markers are applied to the assisted Selection of cotton fiber quality, QTL positioning result is reliable, can accelerate the seed selection process of China's good fiber quality cotton variety.The LOD value of qFL is 222, and interpret table form variation is 55.0%, and additive effect value is 2.66; The LOD value of qFS is 193, and interpret table form variation is 50.2%, and additive effect value is 2.92; The LOD value of qFM is 145, and interpret table form variation is 40.6%, and additive effect value is-0.41.
Accompanying drawing explanation
Fig. 1 is the position of staple length QTL on the 6th karyomit(e) that the embodiment of the present invention provides;
Fig. 2 is the position of fibre strength QTL on the 6th karyomit(e) that the embodiment of the present invention provides;
Fig. 3 is the position of fiber mic value QTL on the 6th karyomit(e) that the embodiment of the present invention provides;
Fig. 4 be the embodiment of the present invention provide QTL region restructuring individual fiber length and mic value performance schematic diagram.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
The embodiment of the present invention with the molecule marker of upland cotton the 6th chromosome fibre quality trait gene linkage, qFL, qFS and qFM and chain molecule marker thereof are all positioned at upland cotton the 6th karyomit(e), particular location is on chromosome as shown in Fig. 1-Fig. 3, with qFL, what qFS and qFM was chain is labeled as PGML3033, NBRI0277, SWU1781, SWU2422, SWU2282, SWU2454, SWU2436, T1, SWU1799, SRAP37F27R, SWU2138, MUSB0103, MUSS122, CGR5355, NAU1218, SWU1794, SWU2098, NAU0874 and SWU06-077.
(1) Fine Mapping colony is set up: according to (cotton No. 1 of T586 × Chongqing) F
2:7the marker genetype of recombinant inbred strain colony strain coloured differently body, the 6th karyomit(e) is selected to provide for T586, and other karyomit(e) (especially the 7th chromosomal brown fibre site, the 12nd chromosomal photon site designation of chromosome section) is for changing the cotton No. 1 strain RIL118 provided, change cotton No. 1 with upland cotton high-quality kind and hybridize, foundation comprises (cotton No. 1 of the RIL118 × Chongqing) F about 6975 strains
2colony;
(2) F is extracted
2the DNA of colony and parent;
(3) the 6th chromosome genetic linkage map is set up: utilize the molecule marker be positioned on the 6th karyomit(e), to (cotton No. 1 of RIL118 × Chongqing) F
2colony carries out marker genetype detection, builds the 6th chromosomal genetic linkage maps, utilizes the molecular marker screening colony parent of new synthesis simultaneously, detects (cotton No. 1 of RIL118 × Chongqing) F
2colony's marker genetype, increase by the 6th chromosomal marker density, the 6th map of structure comprises 154 marks, and overlay length is 92.3cM, and between mark, mean distance is 0.60cM.
(4) Fine Mapping the 6th chromosome fibre quality and ginning outturn QTL: utilize the 6th chromosome genetic linkage map built, in conjunction with (cotton No. 1 of RIL118 × Chongqing) F
2, F
2:3the qualification result of the fiber quality characteristics of colony, Fine Mapping the 6th chromosome fibre length, fibre strength, fiber mic value QTL, obtain 3 fibrous quality QTL on upland cotton the 6th karyomit(e) and linked marker thereof altogether.
Table 1 the 6th chromosome fibre quality QTL
27 of QTL region marks are utilized to carry out genotype detection to 6975 individual plants, obtain the individual plant that 26 restructuring occurs in this region altogether, on the basis of qualification 26 individual fiber quality traits, chromosome segment is adopted to substitute graphing method, by staple length QTL, fibre strength QTL and mic value QTL Fine Mapping to this region T1 location proximate (Fig. 1-Fig. 3), QTL is less than 0.11cM with contiguous mark.
By reference to the accompanying drawings the specific embodiment of the present invention is described although above-mentioned; but not limiting the scope of the invention; one of ordinary skill in the art should be understood that; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various amendment or distortion that performing creative labour can make still within protection scope of the present invention.
Claims (2)
1. one kind with the molecule marker of upland cotton the 6th chromosome fibre quality trait gene linkage, for with upland cotton the 6th chromosome fibre quality trait gene locus qFL, the molecule marker that qFS and qFM is chain, it is characterized in that, be somebody's turn to do and upland cotton the 6th chromosome fibre quality trait gene locus qFL, qFL in the molecule marker that qFS and qFM is chain, qFS and qFM and chain molecule marker thereof are all positioned at upland cotton the 6th karyomit(e), with qFL, what qFS and qFM was chain is labeled as PGML3033, NBRI0277, SWU1781, SWU2422, SWU2282, SWU2454, SWU2436, T1, SWU1799, SRAP37F27R, SWU2138, MUSB0103, MUSS122, CGR5355, NAU1218, SWU1794, SWU2098, NAU0874 and SWU06-077.
2. the molecule marker with the gene linkage of upland cotton the 6th chromosome fibre quality trait according to claim 1, it is characterized in that, the method that this molecule marker chain with upland cotton the 6th chromosome fibre quality trait gene locus qFL, qFS and qFM obtains is as follows:
Step one, sets up Fine Mapping colony: according to cotton No. 1 F in T586 × Chongqing
2:7the marker genetype of recombinant inbred strain colony strain coloured differently body, the 6th karyomit(e) is selected to provide for T586, and other karyomit(e), especially the 7th chromosomal brown fibre site, the 12nd chromosomal photon site designation of chromosome section are the cotton No. 1 strain RIL118 provided that changes, change cotton No. 1 with upland cotton high-quality kind and hybridize, set up cotton No. 1 F in the RIL118 × Chongqing comprising 6975 strains
2colony;
Step 2, extracts F
2the DNA of colony and parent;
Step 3, sets up the 6th chromosome genetic linkage map: utilize the molecule marker be positioned on the 6th karyomit(e), to cotton No. 1 F in RIL118 × Chongqing
2colony carries out marker genetype detection, builds the 6th chromosomal genetic linkage maps, utilizes the molecular marker screening colony parent of new synthesis simultaneously, detects cotton No. 1 F in RIL118 × Chongqing
2colony's marker genetype, increases by the 6th chromosomal marker density;
Step 4, Fine Mapping the 6th chromosome fibre quality and ginning outturn QTL: utilize the 6th chromosome genetic linkage map built, in conjunction with cotton No. 1 F in RIL118 × Chongqing
2, F
2:3the qualification result of the fiber quality characteristics of colony, location the 6th chromosome fibre length, fibre strength, fiber mic value QTL, obtain 3 fibrous quality QTL on upland cotton the 6th karyomit(e) and linked marker altogether.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399575A (en) * | 2016-12-12 | 2017-02-15 | 西南大学 | Molecular markers linked with chlorophyll QTL (Quantitative Trait Loci) genes of gossypium hirsutum |
| CN108359741A (en) * | 2018-05-22 | 2018-08-03 | 山东棉花研究中心 | The one InDel molecular labeling and its application with the 15th chromosome ginning outturn main effect QTL compact linkage of upland cotton |
| CN109762923A (en) * | 2019-03-07 | 2019-05-17 | 中国农业科学院棉花研究所 | With the SSR molecular marker of upland cotton breeding time and plant villus close linkage |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101613761A (en) * | 2009-08-12 | 2009-12-30 | 中国农业科学院棉花研究所 | The SSR mark chain with the cotton fiber strength major gene |
| CN102220318A (en) * | 2011-05-10 | 2011-10-19 | 中国农业科学院棉花研究所 | SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect genes |
-
2014
- 2014-12-03 CN CN201410723250.8A patent/CN104404153A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101613761A (en) * | 2009-08-12 | 2009-12-30 | 中国农业科学院棉花研究所 | The SSR mark chain with the cotton fiber strength major gene |
| CN102220318A (en) * | 2011-05-10 | 2011-10-19 | 中国农业科学院棉花研究所 | SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect genes |
Non-Patent Citations (3)
| Title |
|---|
| DEXIN LIU,ET AL: "Fine mapping and RNA-Seq unravels candidate genes for a major QTL controlling multiple fiber quality traits at the T1 region in upland cotton", 《BMC GENOMICS》 * |
| QUN WAN,ET AL: "T1 locus in cotton is the candidate gene affecting lint percentage, fiber quality and spiny bollworm (Earias spp.) resistance", 《EUPHYTICA》 * |
| 陈利等: "陆地棉遗传图谱构建及产量和纤维品质性状QTL定位", 《作物学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399575A (en) * | 2016-12-12 | 2017-02-15 | 西南大学 | Molecular markers linked with chlorophyll QTL (Quantitative Trait Loci) genes of gossypium hirsutum |
| CN108359741A (en) * | 2018-05-22 | 2018-08-03 | 山东棉花研究中心 | The one InDel molecular labeling and its application with the 15th chromosome ginning outturn main effect QTL compact linkage of upland cotton |
| CN108359741B (en) * | 2018-05-22 | 2021-07-27 | 山东棉花研究中心 | InDel molecular marker closely linked with major QTL (quantitative trait locus) of chromosome 15 of upland cotton and application thereof |
| CN109762923A (en) * | 2019-03-07 | 2019-05-17 | 中国农业科学院棉花研究所 | With the SSR molecular marker of upland cotton breeding time and plant villus close linkage |
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Application publication date: 20150311 |