CN101612193B - Medicine composition used for treating cholecystitis and cholangitis - Google Patents

Abstract

The invention relates to a medicine composition used for treating cholecystitis and cholangitis, belonging to the traditional Chinese medicine. The medicine composition comprises the following active substances according to parts by weight: 20 to 40 parts of andrographolide, 20 to 35 parts of serrate rabdosia aqueous extract and 20 to 40 parts of quassia alcohol extract. The medicine composition is used for treating diseases, such as acute or chronic cholecystitis, cholangitis and the like. The medicine composition has the functions of reducing fever, eliminating dampness and smoothing gallbladder.

Description

A kind of pharmaceutical composition that is used to treat cholecystitis, cholangitis

Technical field

The invention belongs to a kind of Chinese medicine, refer in particular to and be used to treat acute and chronic cholecystitis, cholangitis.

Background technology

Cholecystitis is modal a kind of in the gallbladder disease, the clinical common branch that acute and chronic is arranged.Women's sickness rate is higher, and the age of onset majority is between 20～50 years old, and acute cholecystitis possibly be outbreak for the first time, also possibly on the chronic cholecystitis basis, show effect time and again, and the patient often is face of acute ill during outbreak.Its main clinical manifestation is: stomachache, often betide the evening after the heavy meal, and generally all very violent, be persistence, be the paroxysmal aggravation sometimes, mainly in epigastrium, be transferred to upper right abdomen gradually during beginning, some cases pain can be radiated to right shoulder back.Heating, body temperature is everlasting between 38～39 ℃.Can doublely see simultaneously inappetence, feel sick, gastrointestinal symptoms such as vomiting, abdominal distention and a large amount of belch.Chronic cholecystitis often lacks classical symptom, also can be asymptomatic, if no acute attack history; Often be difficult for making a definite diagnosis, symptom often shows as the abdominal distention that weight differs, and epigastrium or right upper quadrant of the abdomen are uncomfortable; Continue dull pain or right omoplate district pain; Indigestion symptoms such as stomach is scorching hot, belch, pantothenic acid, after the oils food, symptom can increase the weight of on the feed.Motherland's medical science thinks that primary disease is owing to eating and drinking without temperance, greasy food, the fierce elements such as temperature is uncomfortable, feelings will does not freely reach malnutrition due to parasitic infestation of trembling with fear of feed, causes QI stagnation of the liver and galibladder, damp and hot heap soil or fertilizer over and around the roots resistance, leads to and fall not normal forming, and treatment should be with depressed liver-energy dispersing and function of gallbladder promoting, and the dehumidifying of regulating the flow of vital energy is main.

At present the Drug therapy of cholecystitis is used antibiotic morely, but, be difficult to treatment because of problems such as drug resistance and toxic and side effects; Primary disease does not still have ideal treatment medicine at present; Especially to the inhibition of the bacterial infection of gallbladder, promote biliary secretion, human body immunity improving power; Reduce the desirable medicine of recurrence, still remain to be developed.Cholecystitis is a kind of more common surgery acute abdomen, and sickness rate is only second to acute appendicitis.Generally mostly occur in middle age.Say that from theory of medicine cholecystitis can be divided into acute and chronic, but in fact great majority are chronic, begin that disease symptom is very light several times; Pain is not too severe, often is mistaken as gastropathy and is not caused attention, and pathological changes is serious gradually subsequently, under some inducement effect; Show as acute attack, epigastrium keep right limit angor tempestuously, directly waist suddenly; Have to protect abdominal part with hands, dare not bump, even roll on the ground in pain and shout.Have a strong impact on people's life, work, study.Need curative effect rapid, effect is certain for this reason, has no side effect, and can prevent recurrence, but and the medicine taken of longer-term limit.

Summary of the invention

The present invention provides a kind of pharmaceutical composition that is used to treat cholecystitis, cholangitis, is used to treat acute and chronic cholecystitis, cholangitis.

The technical scheme that the present invention takes is: the active substance by following ratio of weight and number is processed:

Andrographolide 20-40 part, Herba Rabdosiae Lophanthoidis water extract 20-35 part, Ramulus Et Folium Picrasmae ethanol extract 20-40 part.

Be preferably:

30 parts of andrographolides, 27.5 parts of Herba Rabdosiae Lophanthoidis water extracts, 30 parts of Ramulus Et Folium Picrasmae ethanol extract.

Above-mentioned various extracts among the present invention can be through method for distilling acquisition arbitrarily in the prior art.

The present invention can be mixed with medicament with one or more pharmaceutically acceptable carriers or adjuvant.

Above-mentioned pharmaceutically acceptable carrier is meant pharmaceutical carrier or the adjuvant that pharmaceutical field is conventional, for example: diluent, excipient and water etc., filler such as starch, dextrin, sucrose, mannitol, lactose, microcrystalline Cellulose etc.; Binding agent such as cellulose derivative, alginate, gelatin and polyvinylpyrrolidone; Wetting agent such as glycerol: disintegrating agent such as methyl starch sodium, hyprolose, cross-linked carboxymethyl cellulose, agar, calcium carbonate and sodium bicarbonate; Absorption enhancer such as quaternary ammonium compound; Surfactant such as hexadecanol, Tween 80, sodium lauryl sulphate; Alms bowl such as Kaolin and soap clay are carried in absorption; Lubricant such as Pulvis Talci, calcium stearate and magnesium, micropowder silica gel and Polyethylene Glycol etc.In medicament, can also contain other adjuvant such as flavouring agent, sweeting agent etc. in addition.

But the mode of medicament administered through oral, rectum or parenteral that the present invention processes is applied to the patient.Be used for when oral, can be made into conventional solid preparation such as tablet, capsule, powder, granule etc., process other liquid preparation of liquid preparation such as water or oil-suspending agent such as syrup, mixture, elixir etc.; When being used for parenteral, can be made into solution, powder pin, water or the oiliness suspending agent etc. of injection.The preferred form of the present invention is oral liquid, tablet, coated tablet, capsule, granule.

The present invention is used to treat diseases such as acute and chronic cholecystitis, cholangitis.Effect with heat clearing away, damp eliminating, function of gallbladder promoting.The amount of application of the present invention and medicament thereof can be according to variations such as the type of route of administration, patient age, body weight, the disease of being treated and the orders of severity, and is oral, one time 6,3 times on the one.

The specific embodiment

Come further checking the present invention through Pharmacodynamic test of active extract below.

1 experiment material

1.1 medicine medicine of the present invention, hereinafter to be referred as XIAOYAN LIDAN PIAN: JiLin ZiXin Pharmacy Co., Ltd provides, the Liandan anti-inflammation capsule, and Shantou, Guangdong pharmaceutical factory provides lot number: 050614.More than two medicines face with preceding and be made into desired concn with the 1%CMC-Na physiological salt liquid.Pentobarbital, intersection dish, Oleum Tiglii proinflammatory agent.

1.2 the animal Kunming mouse, body weight 18～22g, male and female half and half, the Wistar rat, body weight 200 ± 20g, male and female half and half are all available from institute for drug control, Jilin Province animal housing.Be in a good state of health the raising condition: normal illumination, the drinking-water of freely ingesting, 18～25 ℃ of ambient temperatures, humidity 50～70%.

2 methods and result

2.1 antiinflammatory action

2.1.1 50 of healthy mices are chosen in the influence of Oleum Tiglii induced mice auricle edema, be divided into 5 groups at random, 10 every group, male and female half and half, gastric infusion (ig) gives XIAOYAN LIDAN PIAN and Liandan anti-inflammation capsule (positive control) respectively.And make negative control with normal saline, and successive administration 3d, 0.5h after the last administration is coated with Oleum Tiglii proinflammatory agent (2% Oleum Tiglii, 20% dehydrated alcohol, 5% distilled water, 73% ether) 0.1ml in the in ear outside, a mice left side and causes inflammation.Cause scorching back 4h and lay left and right auricle with card punch (aperture 6mm), weigh, as the swelling degree, the result sees table 1 with left and right sides auricle weight difference.

The influence

of table 1 pair Oleum Tiglii induced mice auricle edema

Contrast with the normal saline group ^★P＜0.05, ^{★ ★}P＜0.01; Compare △ P＞0.05 with the Liandan anti-inflammation capsule

2.1.2 40 of healthy rats are chosen in the influence of rat paw edema due to the on Carrageenan, weigh, and are divided into 5 groups at random, and 8 every group, male and female half and half, ig gives XIAOYAN LIDAN PIAN and Liandan anti-inflammation capsule (positive control) negative control group ig gives normal saline respectively.Successive administration 3d, 30min after the last administration causes inflammation at the right back sufficient plantar subcutaneous injection 1% carrageenin suspension 0.1ml/ of rat respectively.Respectively at cause scorching before with cause scorching back 15,30,60,120,240,360min surveys the girth of the right back sufficient sole of the foot of rat, and calculates the swelling rate.The result sees table 2.

2.2 choleretic effect

Get 40 of male rats, be divided into 5 groups after weighing at random, 8 every group, fasting drinking-water 12h before the experiment; During experiment every Mus with pentobarbital sodium 60mg/kg intraperitoneal injection of anesthesia after, face upward the position and be fixed on the fixing head, cut about 2cm along the abdomen median line, open the abdominal cavity; Find stomachus pyloricus, the upset duodenum finds the bile duct of white flexible in the descendant duodenum mesentery, under it, wear 2 lines; The ligation pars papillaris is made " V " notched cut to the liver direction, inserts plastic tube; Promptly visible have pistac bile to flow out, and ligation fixed plastics pipe is collected bile with small beaker.Stable back collection 30min bile, and after duodenal administration is whenever collected bile 1 time at a distance from 30min after the administration, totally 3 times, record bile flow, bile flow increase percentage rate after the calculating administration.The result sees the following form.

The excretory influence of table 3 pair rat bile

Compare with the normal saline group ^★P＜0.05, ^{★ ★}P＜0.01; Compare △ P＞0.05, △ △ P＜0.01 with the Liandan anti-inflammation Capsules group

2.3 antibacterial action

2.3.1 the in-vitro antibacterial test adopts test tube method to investigate the antibacterial action of medicine to common bacteria.Used culture medium is the caseinhydrolysate culture medium, and medicine becomes 9 concentration to get a test tube in addition by doubling dilution not add medicine as blank.Add respectively in 10 test tubes after strain subject cultivated, in 37 ℃ of incubators, cultivate 24h, take out and observe the bacterial growth situation, and observe with plating and to have or not bacterial growth.The result sees table 4～5.

The vitro antibacterial activity of table 4 XIAOYAN LIDAN PIAN

"+" has bacteria growing, "-" asepsis growth

The capsular vitro antibacterial activity of table 5 Liandan anti-inflammation

"+" has bacteria growing, "-" asepsis growth

Not strong from the vitro antibacterial activity of visible XIAOYAN LIDAN PIAN of above result and Liandan anti-inflammation capsule XIAOYAN LIDAN PIAN.Effect is only arranged when high concentration.

2.3.2 antibacterial tests in the body

Adopt clinical isolating bacillus pyocyaneus, after the prerun with 1 * 10 ^-1The every caudal vein injection of concentration 0.2ml, the mouse infection bacillus pyocyaneus is irritated XIAOYAN LIDAN PIAN and the Liandan anti-inflammation capsule suspension successive administration 3d that stomach gives various dose, observed and recorded 7d simultaneously.The every d of model control group mice irritates stomach and gives 1%CMC-Na solution 0.8ml 3d altogether, observed and recorded 7d, and the result sees table 6.

Table 6 XIAOYAN LIDAN PIAN infects the effect of bacillus pyocyaneus to mouse tail vein

With the model group ratio ^★P＜0.05, ^{★ ★}P＜0.01; Compare △ P＞0.05, △ △ P＜0.05 with the Liandan anti-inflammation Capsules group

The visible XIAOYAN LIDAN PIAN of above result can reduce the mortality rate due to the mouse vein infection bacillus pyocyaneus, improves life span and survival rate.

2.4 analgesic activity

Adopt mice acetic acid twisting method.Get 50 of mices, male and female half and half are divided into 5 groups at random, 10 every group.Irritate stomach and give XIAOYAN LIDAN PIAN and Liandan anti-inflammation capsule, successive administration 3d, 30min ip 0.6% acetum 0.2ml/ is only after the last administration.Observe and respectively organize the writhing response number of times that mice occurs in the 10min, the result sees table 7.

The analgesic activity of table 7 pair mice

With N.S group ratio ^★P＜0.05, ^{★ ★}P＜0.01; With the Liandan anti-inflammation Capsules group than △ P＞0.05, △ △ P＜0.05

From the visible XIAOYAN LIDAN PIAN of above result significant analgesic activity is arranged.

Above-mentioned each experimental verification anti-inflammatory and choleretic of the present invention, antibiotic analgesic act as clinical application that the pharmacology is provided foundation.

Embodiment 1

Andrographolide 200g, Herba Rabdosiae Lophanthoidis water extract 200g, Ramulus Et Folium Picrasmae ethanol extract 200g.

Add the conventional adjuvant of preparation granule, process granule.

Above-mentioned various extracts among the present invention can be preferably through method for distilling acquisition arbitrarily in the prior art:

Andrographolide: take by weighing the Herba Andrographis raw material and pulverize in right amount, water decocts 6-17 times of decocting of 1-3 each adding and boils 0.5-3 hour filtration merging filtrate, concentrates.

The Herba Rabdosiae Lophanthoidis water extract: take by weighing the Herba Rabdosiae Lophanthoidis raw material and pulverize in right amount, water decocts 5-14 times of decocting of 1-3 each adding and boils 0.5-3 hour filtration merging filtrate, concentrates.

The Ramulus Et Folium Picrasmae ethanol extract: it is an amount of to take by weighing the Ramulus Et Folium Picrasmae raw material, adds 6-16 times of ethanol and decocts 0.5-3 hour with 50%-90% alcohol reflux 1-3 time is each, filters, and merging filtrate concentrates.

Embodiment 2

Andrographolide 300g, Herba Rabdosiae Lophanthoidis water extract 275g, Ramulus Et Folium Picrasmae ethanol extract 300g.

Add the conventional adjuvant of preparation tablet, process 10000, every heavy 0.3g.

Embodiment 3

Andrographolide 400g, Herba Rabdosiae Lophanthoidis water extract 350g, 400 parts of Ramulus Et Folium Picrasmae ethanol extract.

Add the conventional adjuvant of preparation capsule, encapsulated.

Claims (2)

1. pharmaceutical composition that is used to treat cholecystitis, cholangitis is characterized in that being processed by the active substance of following ratio of weight and number:

Andrographolide 20-40 part, Herba Rabdosiae Lophanthoidis water extract 20-35 part, Ramulus Et Folium Picrasmae ethanol extract 20-40 part.

2. a kind of pharmaceutical composition that is used to treat cholecystitis, cholangitis according to claim 1 is characterized in that being processed by the active substance of following ratio of weight and number:

30 parts of andrographolides, 27.5 parts of Herba Rabdosiae Lophanthoidis water extracts, 30 parts of Ramulus Et Folium Picrasmae ethanol extract.