CN101590075A - The application of polycation disinfectant in disinfecting nipple skins of dairy cows - Google Patents

Abstract

The invention provides polyhexamethylene guanidine, polyhexamethylene and be used for the new purposes that polycation disinfectant such as guanidine carries out disinfection to cow papillary skin before and after milking.This type of disinfectant is nontoxic, non-stimulated, do not have other side effect, do not influence the cow producing milk quality, is applicable to the sterilization of milch cow before and after milking, and can effectively prevent the generation of bovine subclinical mastitis or clinic mastitis.

Description

The application of polycation disinfectant in disinfecting nipple skins of dairy cows

Technical field

The present invention relates to the new purposes that polycation disinfectant such as polyhexamethylene guanidine carries out disinfection to cow papillary skin before and after milking.

Background technology

Mastitis is to cause milk cattle cultivating one of the most serious disease of loss of helping already.The pathogenic microorganism that causes mastitis is caused by staphylococcus, streptococcus and escherichia coli etc. mostly, these pathogenic microorganisms mainly are present in milch cow body surface and the environment, the pathogenic microorganism of cow breast surface contamination can infect mammary gland by the hands of milking machine, the person of milking or the towel of scouring breast etc., causes latent mammitis or clinic mastitis.Both at home and abroad the milk industry generally believes, the agent sterilization that carries out disinfection is a kind of effective ways of anti-in recent years system mammitis of cow to mammilla of milk cattle before and after milking.The requirement of nipple dipping disinfectant is: fungicidal spectrum is wide, sterilization speed is fast, zest is little, drug residue is little.

Be the milk germ contamination that prevents that cost is high widely, people have carried out many effort.Now had many pre-treatment breast of milking improve one's methods and prevent and the method for treatment of cow's milk adenitis (for example referring to United States Patent (USP) 4,206,529, Neumann; No.5,124,145 and 5,234,684, people such as Sordillo; No.4,253,420, Heofelmayr and No.5,366,732, Zighelboim).Other people has described improved antimicrobial compositions, its be used in particular for solving the contaminative residue is arranged in the cleaning course before milking problem (for example referring to U.S. Patent No. 5,139,788, Schmidt).People such as Berg (milk science impurity (J.Dairy Sci.) 68,457-461 (1985)); People such as Pankey (Veterinary Clinics of North America9,519-530,1993); People such as McKinnon (J.Dairy Res.50,153-162,1983); People such as Murdough (J.Dairy Sci.76,2033-2038,1993) and people (Am.J.Infect.Control19,243-249 such as Ansari, 1991) further described the As-Is in this field, and described before general milking the improvement aspect the nipple sterilization.

At present, the mammilla of milk cattle disinfectant of selling on the domestic market is various in style, is traditional disinfectant products such as chlorhexidine, iodophors basically.Although povidone iodine class disinfectant milk milch cow and have certain effect aspect the sterilization (Luo Yan is etc. mammilla of milk cattle appropriative disinfector disinfection effect research [J] for Wang Jingyu, Jiang Xiangyang. Chinese agronomy circular, 2005,21 (1): 1-17); (Yuan Yonglong, Yang Yuying, Meng Jucheng, etc. the development of disinfecting nipple skins of dairy cows agent and screening [J]. Chinese veterinarian's medical magazine, 1994,4:3-5).But have experiment show 1% povidone iodine commonly used milch cow is milked before and after Disinfection Effect only suitable with 0.5% chlorhexidine, referring to L.K.Fox (J.Dairy Sci.75:66-71,1992); People such as J.S.HOGAN (J.Dairy Sci.78:2502-2506,1995).Stronger as sterilizing power, the application of littler, nontoxic, the non-staining polyhexamethylene guanidine of zest in milch cow is milked sterilization but do not appear in the newspapers.The inventor has invented the new purposes that polyhexamethylene guanidine is used for disinfecting nipple skins of dairy cows by a large amount of experimentatioies.

Summary of the invention

This provides content is the application of polycation disinfectant aqueous solutions such as polyhexamethylene guanidine in disinfecting nipple skins of dairy cows of 0.1-1%.

Polycation disinfectants such as polyhexamethylene guanidine can be used as a kind of main component of cow papillary skin appropriative disinfector, the guanidine radicals of positively charged has very high activity, be easy to be usually electronegative each bacterioid, virus is adsorbed, thereby the splitting function that suppresses bacterial virus, make antibacterial, virus forfeiture fertility, even more important and valuable is that polyhexamethylene guanidine is nontoxic, non-stimulated, there is not other side effect, do not influence the cow producing milk quality, be applicable to the sterilization of milch cow before and after milking, can effectively prevent the generation of bovine subclinical mastitis or clinic mastitis.

Embodiment 1

Experiment purpose: laboratory microorganism killing effect

1) test equipment:

1, strain: staphylococcus aureus ATCC6538, escherichia coli 8099

2, constant water bath box

3, measuring pipette (0.1ml, 1.0ml, 5.0ml, 10.0ml)

4, capillary pipette

5, centrifuge

6, motorized agitator.

2) preparation of bacterium sheet:

The bacterium sheet that uses in the sterilizing test is to be added on the carrier with the bacterium drop to make, and before the used carrier microbiological contamination, should carry out ungrease treatment.

Unified with white gambroon making.Before cutting off, earlier the cloth of defat press the size of carrier regulation, take out trimming week through each one of weft yarn, cut off by the trace of drawnworking again.The carrier size that this method is made is consistent, and does not have burr; Carrier with dripping when dying method, is tiled in the cloth sheet through sterilization in the aseptic plate behind pressuresteam sterilization, drips bacterium liquid piecewise.Every of dosage is 0.01-0.02ml.Available 0.1ml suction pipe drips bacterium liquid, and also available 1.0ml syringe connects No. 6 syringe needles and drips bacterium liquid.Put and dry (about 30min) in 37 ℃ of incubators, or put and re-use after drying in the shade naturally under the room temperature; The microbiological contamination of bacterium sheet is 5 * 10 ⁵-5 * 10 ⁶Cfu.

3) operation sequence:

7, get aseptic little plate, indicate inject the concentration of disinfectant solution.By the amount of every 5.0ml, the disinfectant solution of drawing respective concentration injects plate.

8, will fill the disinfectant plate and put in 20 ℃ ± 1 ℃ water-bath behind the 5min, and put into 4 of previously prepared bacterium sheets respectively with aseptic nipper, and make it to soak in disinfectant.

9, treat that the bacterium medicine interacted to each scheduled time, with aseptic nipper with the bacterium sheet take out move into respectively one contain among the 5.0ml and test tube in.Mix 20s with motorized agitator, or, the antibacterial on the bacterium sheet is entered in the neutralizer by eluting, place again more than the 5min, make neutralization abundant test tube rapping 80 times.Behind the final further mixing, draw the 1.0ml direct inoculation in plate, 2 plates of every pipe inoculation are measured and are deposited viable count.

10, get a plate, inject the 10.0ml diluent and replace disinfectant solution, put into 2 bacterium sheets, as positive controls.Its test procedure subsequently is identical with above-mentioned test group with viable bacteria cultivation counting.

11, all test samples are all cultivated in 37 ℃ of incubators, bacterial propagule is cultivated 48h observe final result; Bacterial spore is cultivated 72h observe final result.

12, test repeats 3 times (comprising contrast), calculates the viable bacteria amount (cfu/ sheet) of each group, calculates killing rate.

4) experimental result

1, to the bactericidal effect of staphylococcus aureus

Under 20 ℃ ± 1 ℃ condition, prove that through three repeated trials contain 1.0%, 0.8%, 0.4% Polyhaxemethylenguanidine Hydrochloride aqueous solution, effect 15s, 30s, 60s are respectively 99.97%, 98.89%, 93.86% to the staphylococcus aureus average kill ratio.(table 1.1).

Table 1.1 Polyhaxemethylenguanidine Hydrochloride is to the bactericidal effect of staphylococcus aureus

Annotate: average clump count of positive controls and scope are 3.51 * 10 ⁶(2.42 * 10 ⁶～4.10 * 10 ⁶) the cfu/ sheet, negative control group is an asepsis growth.

2, to colibacillary bactericidal effect

Under 20 ℃ ± 1 ℃ condition, prove through three repeated trials, the sterilizing agent concentration contains 1.0%, 0.8%, 0.4% Polyhaxemethylenguanidine Hydrochloride aqueous solution, acts on 15s, 45s, 60s respectively, and the escherichia coli average kill ratio is respectively 99.97%, 94.62%, 93.06%., escherichia coli are had disinfective action (table 1.2).

Table 1.2 Polyhaxemethylenguanidine Hydrochloride is to colibacillary bactericidal effect

Annotate: average clump count of positive controls and scope are 3.90 * 10 ⁶(3.80 * 10 ⁶～4.00 * 10 ⁶) the cfu/ sheet, negative control group is an asepsis growth.

Embodiment 2

Experiment purpose: skin irritation test

1. experimental technique

(1) experimental animal: each test needs 3 healthy rabbits that skin is intact at least

(2) skin irritation test operation program:

A, decontaminant concentration: 0.5% Polyhaxemethylenguanidine Hydrochloride

B, before test 24h, with depilatory with tame rabbit back spinal column both sides the hair remove, must not injured skin.The unhairing scope, left and right sides Ge Yue 3cm * 3cm.

To be tried C, next day direct the dripping in area of thing (concentration is generally 5 times or the stock solution that skin degerming is used liquid) 0.5ml (g) is on the intact skin of a side of 2.5cm * 2.5cm, or drip on 2 layers of onesize-4 layers of gauze and apply ointment or plaster on a side skin of unhairing surface, cover the non-stimulated immobilization with adhesive tape of reuse with non-stimulated plastic foil of one deck or oilpaper then.The opposite side skin of unhairing is as blank (or solvent control).The time of applying ointment or plaster is 4h.After the off-test, remove the residual thing that tried with warm water or nonirritant solvent.

D, tried behind the thing 1h, 24h and 48h respectively at removal and observe local skin and reflect, and marked.

2. experimental result

Erythema and edema did not all appear in 1h, 24h and each rabbit plaster application side skin of 48h after polyhexamethylene guanidine was applied ointment or plaster, and poisoning symptom also do not occur.The results are shown in Table 1.3.

Table 1.3 polyhexamethylene guanidine is to rabbit skin time irritant reaction

Conclusion: polyhexamethylene guanidine is 0 to the rabbit skin irritation index, and the stimulus intensity rank is a nonirritant.

Claims (3)

1. content is polyhexamethylene guanidine or the poly hexamethylene biguanide aqueous solution of 0.1-1%, before and after milch cow is milked to the application of its disinfecting nipple skins.

2. application according to claim 1 is characterized in that described polyhexamethylene guanidine is Polyhaxemethylenguanidine Hydrochloride, phosphoric acid polyhexamethylene guanidine or sulphuric acid polyhexamethylene guanidine.

3. application according to claim 1 is characterized in that described poly hexamethylene biguanide is hydrochloric polyhexamethylene biguanide, phosphoric acid poly hexamethylene biguanide or sulphuric acid poly hexamethylene biguanide.