CN101556288A - Reagent card for detecting human ABO/RHD blood-grouping and detection method using same - Google Patents
Reagent card for detecting human ABO/RHD blood-grouping and detection method using same Download PDFInfo
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- CN101556288A CN101556288A CNA2009100293552A CN200910029355A CN101556288A CN 101556288 A CN101556288 A CN 101556288A CN A2009100293552 A CNA2009100293552 A CN A2009100293552A CN 200910029355 A CN200910029355 A CN 200910029355A CN 101556288 A CN101556288 A CN 101556288A
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Abstract
The invention discloses a reagent card for detecting human ABO/RHD blood-grouping and a detection method using the same. The reagent card comprises a lower casing and an upper casing, wherein, three parallel grooves are formed in the lower casing; three substrates are arranged in the three grooves respectively; mats and reaction films are stuck on the three substrates; one end of the mat is in mutual lap joint with the reaction film; an anti-A detection line and a control line are arranged on the first reaction film; an anti-B detection line and a control line are arranged on the second reaction film; an anti-RHD detection line and a control line are arranged on the third reaction film; three liquid-dripping openings and three blood-dripping openings corresponding to the three mats are sequentially arranged on the upper casing; three inspection windows corresponding to the three detection lines and the three control lines are formed on the upper casing; and the blood-dripping openings are positioned between the liquid-dripping openings and the inspection windows. The detection method comprises the following steps: blood-dripping, liquid-dripping, erythrocyte migration and chromatography and result observation, therefore, the invention has the advantages of shorter detection time, higher detection accuracy, lower cost and more convenient detection and observation.
Description
Technical field:
The present invention relates to a kind of reagent card that detects blood typing and with the method for its detection, particularly a kind of reagent card that detects people ABO and RHD blood typing and with the method for its detection is applicable to hospital, family.
Background technology:
Erythrocyte blood type is to divide according to the erythrocyte surface antigen in the blood, and according to the difference of antigen type, common have more than 20 categorizing system, and ABO blood group system is one of them.The abo blood group principle of classification is: no A and Blood group antigen B on the red blood cell, and anti-A and anti-B antibody person are arranged in the serum, be called the O type; A antigen is arranged on the red blood cell, and the person that has the anti-B antibody in the serum is called the A type; B antigen is arranged on the red blood cell, and the person that has the anti-A antibody in the serum is called Type B; A and B antigen are arranged on the red blood cell, and nonreactive A and anti-B antibody person in the serum are called the AB type.The biosynthesizing of H antigen is prior to A and B antigen, so except that variant, all there are H antigen in A type, Type B, AB type, O type red blood cell.A type, Type B, H antigen do not exist only in red blood cell, are present in histocyte yet; The RH blood group is after abo blood group is found, the maximum blood group of clinical meaning also is a most complicated blood group system, and it has 6 genes, 5 kinds of antigens, and CCDEe totally 18 kinds of phenotypes, and 36 kinds of genotypes, wherein D antigen is the strongest.
Be mainly used in ABO at present and the RHD blood grouping reagent mainly adopts agglutination and micro-column gel agglutination assay, adopt the agglutination reagent box of agglutination not to be with and contrast, the result judges can not standardization, and sensitivity is relatively poor, can not detect the D blood group; When detecting, micro-column gel agglutination assay needs special gel cards, the gel cost is than higher, the calorie requirement particular manufacturing craft, be equipped with simultaneously special centrifugal machine make this method detect the blood group running time need 30-40 minute long, experimental cost is higher, a kind of detection method is arranged at present in addition, anti-A detection line, anti-B detection line, anti-RHD detection line and nature controlling line are promptly arranged on responding layer, when if blood is the AB type, red blood cell in the blood is most of can be by anti-A detection line absorption, anti-B detection line is little red, can be that the tester suspects testing result like this, and the testing result out of true.
Summary of the invention:
The objective of the invention is in order to overcome above deficiency, provide a kind of detection time shorter, it is higher to detect degree of accuracy, and cost is lower, detect and observe more convenient, be fit to a kind of reagent card and detection method thereof that detects people ABO and RHD blood typing that hospital and family use.
Purpose of the present invention is achieved through the following technical solutions: the reagent card of a kind of people of detection ABO and RHD blood typing, comprise lower house, upper shell, in lower house, be provided with three parallel grooves, and be respectively equipped with a substrate in three grooves, all be pasted with mat and reaction film on three substrates, one end and the reaction film of mat overlap mutually, three on-chip reaction films are respectively first reaction film, second reaction film, the 3rd reaction film, on first reaction film, be provided with anti-A detection line and nature controlling line, on second reaction film, be provided with anti-B detection line and nature controlling line, on the 3rd reaction film, be provided with anti-RHD detection line and nature controlling line, and be provided with successively on the upper shell and three corresponding three liquid droping ports of mat and three mouths of bleeding, upper shell is provided with and three detection lines and three corresponding three visual windows of nature controlling line, bleeds mouth between liquid droping port and visual windows.
Further improvement of the present invention is: substrate is made by plastics or PVC material.
Further improvement of the present invention is: anti-A detection line is anti-A monoclonal antibody reagent, and anti-B detection line is anti-B monoclonal antibody reagent, and anti-RHD detection line is anti-D monoclonal antibody reagent.
Further improvement of the present invention is: nature controlling line is a Quality Control polyclone anti erythrocyte antibody.
A kind of detection method that detects people ABO and RHD blood grouping reagent card of using may further comprise the steps:
A, drip 0.01ml～0.02ml by bleeding mouthful blood sample on three mats;
B, drip 2-3 respectively at three liquid droping ports and drip buffering agent;
C, red blood cell move chromatography by capillary action on reaction film;
D, again by three corresponding in three visual windows detection lines and the variable color of three nature controlling lines, judge testing result.
Description of drawings:
Fig. 1 is a structural representation of the present invention;
Number in the figure: 1-lower house, 2-upper shell, 3-mat, 4-first reaction film, the anti-A detection line of 5-, 6-nature controlling line, 7-second reaction film, the anti-B detection line of 8-, 9-the 3rd reaction film, the anti-RHD detection line of 10-, 11-liquid droping port, 12-bleed mouthful, the 13-visual windows.
Embodiment:
In order to deepen the understanding of the present invention, the invention will be further described below in conjunction with embodiment and accompanying drawing, and this embodiment only is used to explain the present invention, does not constitute the qualification to protection domain of the present invention.
Show the reagent card of a kind of people of detection ABO of the present invention and RHD blood typing and with the embodiment of the method for its detection as Fig. 1, comprise lower house 1, upper shell 2, in lower house 1, be provided with three parallel grooves, in three grooves, be respectively equipped with a substrate, and substrate is made by plastics or PVC material, and all be pasted with mat 3 and reaction film on three substrates, reaction film has good hydrophilicity, one end and the reaction film of mat 3 overlap mutually, three on-chip reaction films are respectively first reaction film 4, second reaction film 7, the 3rd reaction film 9, on first reaction film 4, be provided with anti-A detection line 5 and nature controlling line 6, on second reaction film 7, be provided with anti-B detection line 8 and nature controlling line 6, on the 3rd reaction film 9, be provided with anti-RHD detection line 10 and nature controlling line 6, and anti-A detection line 5 is anti-A monoclonal antibody reagent, anti-B detection line 8 is anti-B monoclonal antibody reagent, anti-RHD detection line 10 is anti-D monoclonal antibody reagent, nature controlling line 6 is Quality Control polyclone anti erythrocyte antibody, on upper shell 2, be provided with successively and three mats, 3 corresponding three liquid droping ports 11 and three mouths 12 of bleeding, upper shell 2 is provided with and three detection lines 5,8, article 10 and three, nature controlling line 6 corresponding three visual windows 13 are bled mouth 12 between liquid droping port 11 and visual windows 13.A kind of detection method that detects people ABO and RHD blood grouping reagent card may further comprise the steps: a, blood bled by three mouthfuls 12 drips the blood sample of 0.01ml～0.02ml respectively on three mats 3; B, drip 2-3 respectively by three liquid droping ports 11 again and drip buffering agent on mat 3, can prevent erythrocyte hemolysis; C, red blood cell move chromatography by capillary action on reaction film; D, again by the variable color of 5,8,10 and three nature controlling lines 6 of three detection lines corresponding in three visual windows 13, judge testing result.If the anti-A detection line 5 on first reaction film 4 is red, nature controlling line 6 also is red, and reactionless on other reaction film, then is A type blood; If the anti-B detection line 8 on second reaction film 7 is red, nature controlling line 6 also is red, and reactionless on other reaction film, then is Type B blood; If the anti-B detection line 8 on the anti-A detection line 5 on first reaction film 4 and second reaction film 7 all is red, nature controlling line 6 also is red, and reactionless on the 3rd reaction film 9, then is AB type blood; If the anti-B detection line 8 on the anti-A detection line 5 on first reaction film 4 and second reaction film 7 all is colourless, nature controlling line 6 is red, and reactionless on the 3rd reaction film 9, then is O type blood; As long as the anti-RHD detection line 10 on the 3rd reaction film 9 be red, nature controlling line 6 also be a redness, and then blood group is the RH positive, or the anti-RHD detection line 10 on the 3rd reaction film 9 is colourless, and nature controlling line 6 is a redness, and then blood group is the RH feminine gender; As long as nature controlling line 6 is colourless, then detect failure.By detect detecting people ABO and RHD blood typing with the migration chromatography, not only make its detection time shorter like this, it is higher to detect degree of accuracy, and cost is lower, detects and observe more convenient, is fit to family, hospital uses.
Claims (5)
1, a kind of reagent card that detects people ABO and RHD blood typing, it is characterized in that: comprise lower house (1), upper shell (2), be provided with three parallel grooves in the described lower house (1), be respectively equipped with a substrate in described three grooves, all be pasted with mat (3) and reaction film on described three substrates, one end and the reaction film of described mat (3) overlap mutually, three on-chip reaction films are respectively first reaction film (4), second reaction film (7), the 3rd reaction film (9), on first reaction film (4), be provided with anti-A detection line (5) and nature controlling line (6), on second reaction film (7), be provided with anti-B detection line (8) and nature controlling line (6), on the 3rd reaction film (9), be provided with anti-RHD detection line (10) and nature controlling line (6), be provided with successively on the described upper shell (2) and corresponding three liquid droping ports of three mats (3) (11) and three bleed mouthful (12), described upper shell (2) is provided with and described three detection lines (5,8,10) and corresponding three visual windows of three nature controlling lines (6) (13), described bleed mouthful (12) are between liquid droping port (11) and visual windows (13).
2, according to the reagent card of claim 1 described detection people ABO and RHD blood typing, it is characterized in that: described substrate is made by plastics or PVC material.
3, according to the described reagent card that detects people ABO and RHD blood typing of claim 1, it is characterized in that: described anti-A detection line (5) is anti-A monoclonal antibody reagent, described anti-B detection line (8) is anti-B monoclonal antibody reagent, and described anti-RHD detection line (10) is anti-D monoclonal antibody reagent.
4, according to the reagent card of claim 1 described detection people ABO and RHD blood typing, it is characterized in that: described nature controlling line (6) is a Quality Control polyclone anti erythrocyte antibody.
5, according to the described method that detects the use detection of people ABO and RHD blood grouping reagent card of claim 1, it is characterized in that, may further comprise the steps:
A, drip the blood sample of 0.01ml～0.02ml on three mats (3) by bleed mouthful (12);
B, drip 2-3 respectively at three liquid droping ports (11) and drip buffering agent;
C, red blood cell move chromatography by capillary action on reaction film;
D, again by three detection lines (5,8,10) corresponding in three visual windows (13) and the variable color of three nature controlling lines (6), judge testing result.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100293552A CN101556288A (en) | 2009-04-10 | 2009-04-10 | Reagent card for detecting human ABO/RHD blood-grouping and detection method using same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100293552A CN101556288A (en) | 2009-04-10 | 2009-04-10 | Reagent card for detecting human ABO/RHD blood-grouping and detection method using same |
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| CN101556288A true CN101556288A (en) | 2009-10-14 |
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| CNA2009100293552A Pending CN101556288A (en) | 2009-04-10 | 2009-04-10 | Reagent card for detecting human ABO/RHD blood-grouping and detection method using same |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103439518A (en) * | 2013-06-07 | 2013-12-11 | 中国人民解放军南京军区南京总医院 | Blood ABD typing rapid comprehensive test card |
| TWI572330B (en) * | 2015-12-28 | 2017-03-01 | 國立中興大學 | Device for blood typing |
| CN107142531A (en) * | 2017-07-19 | 2017-09-08 | 广东工业大学 | A kind of electrostatic spinning injection apparatus |
| CN111458524A (en) * | 2020-03-26 | 2020-07-28 | 英科新创(厦门)科技股份有限公司 | IgG blood group antibody titer rapid detection kit |
| CN114563583A (en) * | 2022-04-24 | 2022-05-31 | 天津德祥生物技术有限公司 | High-flux micro-reaction blood type positive setting detection device |
| CN115684611A (en) * | 2022-12-30 | 2023-02-03 | 天津德祥生物技术股份有限公司 | Erythrocyte antigen detection test strip and application |
-
2009
- 2009-04-10 CN CNA2009100293552A patent/CN101556288A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103439518A (en) * | 2013-06-07 | 2013-12-11 | 中国人民解放军南京军区南京总医院 | Blood ABD typing rapid comprehensive test card |
| CN103439518B (en) * | 2013-06-07 | 2015-09-02 | 中国人民解放军南京军区南京总医院 | A kind of blood ABD shapes rapid integrated test card |
| TWI572330B (en) * | 2015-12-28 | 2017-03-01 | 國立中興大學 | Device for blood typing |
| CN107142531A (en) * | 2017-07-19 | 2017-09-08 | 广东工业大学 | A kind of electrostatic spinning injection apparatus |
| CN111458524A (en) * | 2020-03-26 | 2020-07-28 | 英科新创(厦门)科技股份有限公司 | IgG blood group antibody titer rapid detection kit |
| CN114563583A (en) * | 2022-04-24 | 2022-05-31 | 天津德祥生物技术有限公司 | High-flux micro-reaction blood type positive setting detection device |
| CN115684611A (en) * | 2022-12-30 | 2023-02-03 | 天津德祥生物技术股份有限公司 | Erythrocyte antigen detection test strip and application |
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Application publication date: 20091014 |