CN103439518A - Blood ABD typing rapid comprehensive test card - Google Patents
Blood ABD typing rapid comprehensive test card Download PDFInfo
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- CN103439518A CN103439518A CN2013102274957A CN201310227495A CN103439518A CN 103439518 A CN103439518 A CN 103439518A CN 2013102274957 A CN2013102274957 A CN 2013102274957A CN 201310227495 A CN201310227495 A CN 201310227495A CN 103439518 A CN103439518 A CN 103439518A
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Abstract
The invention discloses a blood ABD typing rapid comprehensive test card. A card seat (21) of the test card is provided with a plurality of card slots; the plurality of card slots are divided in to a plurality of groups arranged in order; the plurality of card slots are corresponding to a blood group forward typing test region (1), a blood group reverse typing test region (2) and a blood cross-matching test region (3) in sequence; each card slot is provided with a test strip matching the test region thereof; and a card cover is opened with a sampling hole (6) and a flushing hole (7) successively on the positions of each test strip corresponding to the blood group forward typing test region (1), the blood group reverse typing test region (2) and the blood cross-matching test region (3) on the card seat (21). The test card integrates blood group forward typing test, reverse typing test and blood cross-matching test on the same card for simultaneous tests, is short in test time, convenient for carrying, simple in operation, accurate and reliable in results, is suitable for being used in comprehensive tests of primary blood screening and blood group typing, and can also be applied in blood group typing test before transfusion in an emergency military period.
Description
Technical field
The invention belongs to the blood testing technical field, be specifically related to a kind of blood ABD rapid integrated test card of finalizing the design.
Background technology
Current Blood grouping reagent strip on the market unitem often detects, and it is long to exist detection time, and testing cost is high, the shortcoming of inconvenient operation.Positive definite form detects the agglutinations that adopt at present more, and complicated operation, easily pollute.And reverse type detects employing test tube method or flat band method usually, the reagent red blood cell used is mainly prepared with blood unit voluntarily by each, there is no unified standard, the preparation method of constituent parts is also different, alserver's solution composition and physicochemical property are all variant, cause the quality of reverse type red blood cell reagent extremely unstable, and external storage life are short, be generally 1-3 month, this has greatly limited its application in actual clinical.The cross matching common method has the salt water law, pawpaw enzyme process, cohesion amine method, antiglobulin method, micro-column gel agglutination assay.The salt water law is not easy to detect incomplete antibody, the pawpaw enzyme process, and cohesion amine method, antiglobulin method, the micro-column gel agglutination assay reaction time is long, and complex operation is expensive, relates to instrument and uses.
Summary of the invention
The purpose of this invention is to provide a kind of blood group positive definite form detection of having contained, reverse type detects the blood ABD that is easy to interpretation blood examination typing testing result detected with the cross matching rapid integrated test card of finalizing the design, this test card is without instrument, simple to operate, can be in 10min the naked eyes sentence read result, detection time is short, and result accurately and reliably.
The objective of the invention is to realize in the following manner:
A kind of blood ABD rapid integrated test card of finalizing the design, this test card comprises deck and is fastened on the Ka Gai on deck, be provided with a plurality of draw-in grooves on this deck, the plurality of draw-in groove is divided into many groups that sequentially arrange, many group draw-in grooves, sequentially corresponding to blood group positive definite form detection zone, blood group reverse type detection zone and cross matching detection zone, are provided with the test strips that is matched with its detection zone in each draw-in groove; All have well and flushing port on the position of this Ka Gai blood group positive definite form detection zone, blood group reverse type detection zone and each test strips of cross matching detection zone on corresponding to deck.
The plurality of draw-in groove is 6 that are arranged in parallel, it comprises the first draw-in groove, the second draw-in groove, the 3rd draw-in groove, the 4th draw-in groove, the 5th draw-in groove, the 6th draw-in groove, this first draw-in groove, the second draw-in groove and the 3rd draw-in groove are corresponding to blood group positive definite form detection zone, the 4th draw-in groove, the 5th draw-in groove are corresponding to blood group reverse type detection zone, and the 6th draw-in groove is corresponding to the cross matching detection zone.
Also have result and show hole on the position of Ka Gai each test strips of blood group positive definite form detection zone on corresponding to deck.Also have result and show hole and product identification label hole on the position of Ka Gai each test strips of blood group reverse type detection zone on corresponding to deck.
The test strips of cross matching detection zone comprises that thieving paper, first cushion pad, second cushion pad, antibody pad, washing fluid drip pad and as the polyester sheet of backing, washing fluid drips pad and is placed on the polyester sheet edge, one end of first cushion pad rides over washing fluid and drips on pad, the antibody pad rides on the other end of first cushion pad, one end of second cushion pad rides on the antibody pad, and thieving paper rides on the other end of second cushion pad.
On test strips, the position at antibody pad place is corresponding to the well of Ka Gai, and the position at washing fluid application of sample pad place is corresponding to the flushing port of Ka Gai.
Thieving paper, washing fluid application of sample pad, first cushion pad, second cushion pad, antibody cushion material are made by the porous polymer materials with certain pore size, and pore size at least allows single red blood cell to pass through.Described hole macromolecular material is preferably glass fibre.
Add the whole blood sample of 10ul in the time of detection in each project in corresponding well, then drip the auxiliary liquid of 2 to 3 (about 100ul-150ul) in corresponding flushing port, after 10 minutes, get final product sentence read result.
What the blood group positive definite form detected employing is the solid phase detection method, and blood cell protein on fiberglass packing is coated, carry out the Preliminary detection judgement by reacted change color to the blood group of human whole blood.The interpretation mode of positive definite form detection zone is: the redness of well is not taken off, and means that examined samples for example, with corresponding blood group antigens: A in Fig. 1, and B, the D item is all aobvious red, represents that examined samples positive definite form testing result is blood group AB type, the RhD positive.The interpretation mode of reverse type detection zone is: C line color band, mean that this detects effectively, obvious colour band appears in the T line, represent that examined samples are with corresponding antibody, for example the A item shows colour band, the B item does not show colour band, represents that examined samples are with A antibody, and blood group reverse type testing result is the blood group Type B.
What the blood group reverse type detected employing is Colloidal Gold, the blood group antigens of colloid gold label are fixed on glass fibre membrane, during reaction, antibody in blood and its reaction bonded form compound, react again with the blood group antigens on being fixed on nitrocellulose membrane by chromatography, form immune complex, therefore, observe the formation colour band whether antigen antibody complex is arranged on detection line, can carry out the blood typing interpretation.
The methodology that the cross matching method applies to fast is the solid phase detection method, the solid phase detection method is a kind of biological respinse method of carrying out on solid phase carrier, change color after can reacting on carrier by reactant or composition change, and testing result is carried out to interpretation.
The present invention can use instrument to carry out interpretation as a result, and can select some combinations wherein to be tested, and also the blood group reverse type can be detected to the collaurum detection strip used and unite two into one, and a colloid gold test strip carries out anti-A antibody and anti-B antibody detection simultaneously.
Beneficial effect of the present invention compared with the prior art: the present invention detects the blood group positive definite form, the blood group reverse type detects, blood cross matching detects and comprehensively detects to a card simultaneously, drew the blood screening testing result in ten minutes, detection time is short, easy to carry, simple to operate, blood examination typing testing result accurately and reliably, preliminary blood screening when being applicable to take a blood sample and the comprehensive detection of blood typing, also can be used for the front blood typing test of very military period or major disaster blood transfusion in period.
The accompanying drawing explanation
Fig. 1 is blood typing comprehensive detection card card face structural representation.
Fig. 2 is blood typing comprehensive detection card deck structural representation.
Fig. 3 is cross matching test strip structural representation.
In figure, 1 is blood group positive definite form detection zone; 2 is blood group reverse type detection zone; 3 is that cross matching detection zone, 4 is that product identification label hole, 6 is that well, 7 is flushing port for result shows hole, 5,8 is that polyester sheet, 9 is that thieving paper, 10 is that first cushion pad, 12 is that second cushion pad, 13 is the antibody pad for washing fluid drips pad, 11,14 is the first draw-in groove, 15 is the second draw-in groove, and 16 is the 3rd draw-in groove, and 17 is the 4th draw-in groove, 18 is the 5th draw-in groove, 19 is the 6th draw-in groove, and 20 is Ka Gai, and 21 is deck.
Embodiment
Below by specific embodiment, the present invention is further elaborated:
As Figure 1-3, a kind of blood ABD rapid integrated test card of finalizing the design, this test card comprises deck 21 and is fastened on the card lid 20 on deck 21, be provided with a plurality of draw-in grooves on this deck 21, the plurality of draw-in groove is divided into many groups that sequentially arrange, many group draw-in grooves, sequentially corresponding to blood group positive definite form detection zone 1, blood group reverse type detection zone 2 and cross matching detection zone 3, are provided with the test strips that is matched with its detection zone in each draw-in groove; Have successively well 6 and flushing port 7 on the position of this card lid 20 blood group positive definite form detection zone 1, blood group reverse type detection zone 2 and cross matching detection zone 3 each test strips on corresponding to deck 21.
The plurality of draw-in groove is 6 that are arranged in parallel, it comprises the first draw-in groove 14, the second draw-in groove 15, the 3rd draw-in groove 16, the 4th draw-in groove 17, the 5th draw-in groove 18 and the 6th draw-in groove 19, this first draw-in groove 14, the second draw-in groove 15 and the 3rd draw-in groove 16 are corresponding to blood group positive definite form detection zone 1, the 4th draw-in groove 17, the 5th draw-in groove should be in blood group reverse type detection zone 2, the six draw-in grooves 19 corresponding to cross matching detection zones 3 to 18; Cover 20 at card and also have result demonstration hole 4 on the position of blood group positive definite form detection zone 1 each test strips on corresponding to deck 21.Cover 20 at card and also have result demonstration hole 4 and product identification label hole 5 on the position of blood group reverse type detection zone 2 each test strips on corresponding to deck 21.
The test strips of cross matching detection zone 3 comprises that thieving paper 9, first cushion pad 11, second cushion pad 12, antibody pad 13, washing fluid drip pad 10 and as the polyester sheet 8 of backing, washing fluid drips pad 10 and is placed on polyester sheet 8 edges, one end of first cushion pad 11 rides over washing fluid and drips on pad 10, antibody pad 13 rides on the other end of first cushion pad 11, one end of second cushion pad 12 rides on antibody pad 13, and thieving paper 9 rides on the other end of second cushion pad 12.
On test strips, the well 6 on 20, the flushing port 7 that the position of washing fluid application of sample pad 10 covers corresponding to card are covered corresponding to card in the position at antibody pad 13 places.Thieving paper 9, washing fluid application of sample pad 10, first cushion pad 11, second cushion pad 12, antibody pad 13 are made by the porous polymer materials with certain pore size, and pore size at least allows single red blood cell to pass through.Described hole macromolecular material is preferably glass fibre.
Add the whole blood sample of 10ul in the time of detection in each project in corresponding well, drip afterwards the auxiliary liquid of 2 to 3 (about 100ul-150ul) in corresponding flushing port, after 10 minutes, get final product sentence read result.
Adopt said structure, the present invention detects the blood group positive definite form, and reverse type detects, cross matching detects and comprehensively detects to a card simultaneously, draws the blood screening testing result in ten minutes, and detection time is short, easy to carry, simple to operate, blood examination typing testing result accurately and reliably.
What the blood group positive definite form detected employing is the solid phase detection method, and blood cell protein on fiberglass packing is coated, carry out the Preliminary detection judgement by reacted change color to the blood group of human whole blood.The interpretation mode of positive definite form detection zone is: the redness of well is not taken off, and means that examined samples for example, with corresponding blood group antigens: A in Fig. 1, and B, the D item is all aobvious red, represents that examined samples positive definite form testing result is blood group AB type, the RhD positive.The interpretation mode of reverse type detection zone is: C line color band, mean that this detects effectively, obvious colour band appears in the T line, represent that examined samples are with corresponding antibody, for example the A item shows colour band, the B item does not show colour band, represents that examined samples are with A antibody, and blood group reverse type testing result is the blood group Type B.
What the blood group reverse type detected employing is Colloidal Gold, the blood group antigens of colloid gold label are fixed on glass fibre membrane, during reaction, antibody in blood and its reaction bonded form compound, react again with the blood group antigens on being fixed on nitrocellulose membrane by chromatography, form immune complex, therefore, observe the formation colour band whether antigen antibody complex is arranged on detection line, can carry out the blood typing interpretation.
It is the solid phase detection method that cross matching detects the methodology applied to, be coated with anti-IgM on test-strips, IgG, the first adds sample loading mode, during the master reaction: first add blood donor's serum (or blood plasma or whole blood) at well, reaction a period of time, add blood donor's red blood cell or (whole blood), reaction a period of time, in flushing port (being test-strips one end), add washing fluid; During inferior side reaction: first add blood donor's serum (or blood plasma or whole blood) at well, reaction a period of time, add blood donor's red blood cell or (whole blood), reaction a period of time, in flushing (hole is test-strips one end), add washing fluid; Make not red blood cell with antibody response under the springing up of washing fluid away from adding the sampling point place, and be left in and add the sampling point place with the red blood cell of antibody generation immune agglutination, then can react according to the residual judgement antibody of the red blood cell whether aggegation is arranged on reaction site and red blood cell.If master reacts, just can not transfuse blood, inferior side reacts and depending on circumstances determines blood transfusion again.The second adds sample loading mode, blood donor's whole blood first is placed in test tube and reacts a period of time with blood donor's red blood cell with blood donor's red blood cell or blood donor's serum with blood donor's whole blood or blood donor's serum, get again a certain amount of red blood cell mixed liquor in test tube to well (be test-strips add sampling point place), reaction a period of time, in flushing port (being test-strips one end), add washing fluid; Make not red blood cell with antibody response under the springing up of washing fluid away from adding the sampling point place, and be left in and add the sampling point place with the red blood cell of antibody generation immune agglutination, then can react according to the residual judgement antibody of the red blood cell whether aggegation is arranged on reaction site and red blood cell, if reactionless, can transfuse blood, the master reaction can not be transfused blood, and inferior side reaction depending on circumstances determines blood transfusion again.
Claims (7)
1. a blood ABD rapid integrated test card of finalizing the design, this test card comprises deck (21) and is fastened on the Ka Gai (20) on deck (21), this deck is provided with a plurality of draw-in grooves on (21), the plurality of draw-in groove is divided into many groups that sequentially arrange, it is characterized in that, many group draw-in grooves, sequentially corresponding to blood group positive definite form detection zone (1), blood group reverse type detection zone (2) and cross matching detection zone (3), are provided with the test strips that is matched with its detection zone in each draw-in groove; This Ka Gai (20) all has well (6) and flushing port (7) on the position corresponding to upper blood group positive definite form detection zone (1), blood group reverse type detection zone (2) and each test strips of cross matching detection zone (3) of deck (21).
2. the blood ABD according to claim 1 rapid integrated test card of finalizing the design, it is characterized in that, the plurality of draw-in groove is 6 that are arranged in parallel, it comprises the first draw-in groove (14), the second draw-in groove (15), the 3rd draw-in groove (16), the 4th draw-in groove (17), the 5th draw-in groove (18) and the 6th draw-in groove (19), this first draw-in groove (14), the second draw-in groove (15) and the 3rd draw-in groove (16) are corresponding to blood group positive definite form detection zone (1), the 4th draw-in groove (17), the 5th draw-in groove should be in blood group reverse type detection zone (2) to (18), the 6th draw-in groove (19) is corresponding to cross matching detection zone (3).
3. the blood ABD according to claim 1 rapid integrated test card of finalizing the design, is characterized in that, has result at Ka Gai (20) and show hole (4) on the position corresponding to upper each test strips of blood group positive definite form detection zone (1) of deck (21).
4. the blood ABD according to claim 1 rapid integrated test card of finalizing the design, it is characterized in that, have result at Ka Gai (20) and show hole (4) and product identification label hole (5) on the position corresponding to upper each test strips of blood group reverse type detection zone (2) of deck (21).
5. the blood ABD according to claim 1 rapid integrated test card of finalizing the design, the test strips that it is characterized in that cross matching detection zone (3) comprises thieving paper (9), first cushion pad (11), second cushion pad (12), antibody pad (13), washing fluid drips pad (10) and as the polyester sheet (8) of backing, washing fluid drips pad (10) and is placed on polyester sheet (8) edge, one end of first cushion pad (11) rides over washing fluid and drips on pad (10), antibody pad (13) rides on the other end of first cushion pad (11), one end of second cushion pad (12) rides on antibody pad (13), thieving paper (9) rides on the other end of second cushion pad (12).
6. the blood ABD according to claim 5 rapid integrated test card of finalizing the design, it is characterized in that the position at antibody pad (13) place on test strips is corresponding to the well (6) on Ka Gai (20), the position at washing fluid application of sample pad (10) place is corresponding to the flushing port (7) on Ka Gai (20).
7. the blood ABD according to claim 5 rapid integrated test card of finalizing the design, it is characterized in that thieving paper (9), washing fluid application of sample pad (10), first cushion pad (11), second cushion pad (12), antibody pad (13) made by the porous polymer materials with certain pore size, pore size at least allows single red blood cell to pass through.
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Cited By (6)
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| CN103364571A (en) * | 2013-06-24 | 2013-10-23 | 英科新创(厦门)科技有限公司 | Cross-matching detection method and test strip |
| CN103926400A (en) * | 2014-04-06 | 2014-07-16 | 邵超鹏 | Test strip for specifically measuring IgM, IgG and IgA blood group antibodies |
| CN109459575A (en) * | 2018-09-19 | 2019-03-12 | 厦门为正生物科技股份有限公司 | A kind of ABO&RhD blood group detection device |
| CN112578129A (en) * | 2020-12-05 | 2021-03-30 | 西安良升生物科技有限公司 | ABO positive and negative typing/RhD blood type detection micro-disc |
| CN114324957A (en) * | 2022-03-16 | 2022-04-12 | 天津德祥生物技术有限公司 | Blood type positive and negative shaping sample adding card and sample adding assembly |
| WO2023039938A1 (en) * | 2021-09-14 | 2023-03-23 | 南京岚煜生物科技有限公司 | Microfluidic blood type test chip |
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| CN112578129A (en) * | 2020-12-05 | 2021-03-30 | 西安良升生物科技有限公司 | ABO positive and negative typing/RhD blood type detection micro-disc |
| WO2023039938A1 (en) * | 2021-09-14 | 2023-03-23 | 南京岚煜生物科技有限公司 | Microfluidic blood type test chip |
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| CN114324957B (en) * | 2022-03-16 | 2022-05-20 | 天津德祥生物技术有限公司 | Blood type positive and negative shaping sample adding card and sample adding assembly |
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Address after: 210002 Zhongshan East Road, Jiangsu, China, No. 305, No. Co-patentee after: Yingke Xinchuang (Xiamen) Science and Technology Co., Ltd. Patentee after: Nanjing General Hospital of Nanjing Military Region of the People's Liberation Army of China Address before: 210002 Zhongshan East Road, Jiangsu, China, No. 305, No. Co-patentee before: Yingke Xinchuang (Xiamen) Technology Co., Ltd. Patentee before: Nanjing General Hospital of Nanjing Military Region of the People's Liberation Army of China |