CN101537169A - Transfer factor solution for livestock and poultry, and preparation method thereof - Google Patents
Transfer factor solution for livestock and poultry, and preparation method thereof Download PDFInfo
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- CN101537169A CN101537169A CN200810052451A CN200810052451A CN101537169A CN 101537169 A CN101537169 A CN 101537169A CN 200810052451 A CN200810052451 A CN 200810052451A CN 200810052451 A CN200810052451 A CN 200810052451A CN 101537169 A CN101537169 A CN 101537169A
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Abstract
The invention provides a transfer factor solution for livestock and poultry. Frozen pig spleens are washed clean by use of distilled water, cut into small pieces, added with distilled water free from heat source, subjected to cell disruption by use of a tissue bruiser and prepared into homogenate; the homogenate passes through a high-pressure homogenizer under the pressure of 60 kPa and then is added with a flocculating agent for flocculation; the flocculated homogenate is centrifuged at a centrifugation ambient temperature of 4 DEG C and at a speed of 7,000 r/min; precipitate is removed; supernatant is left on standby; the supernatant is filtered by use of a microporous filtering membrane 0.45 mu m in pore diameter, and then is ultrafiltered by use of a ultrafiltration membrane 5,000 Dalton in molecular weight cutoff so as to obtain a stock solution; after viruses are removed, nicotinamide is added as a stabilizer; the stock solution is subjected to aseptic filtration by use of the microporous filtering membrane; and the obtained product is added with glycerol as a heat-resisting protective agent and then is directly and separately packed. The transfer factor solution can be applied to livestock and poultry. The preparation method is characterized by directly using the pig spleen to extract the transfer factor solution so as to save manpower and materials and improve extraction efficiency. The preparation method combines a heat-resisting protection technique with transfer factors, thereby realizing the normal-temperature preservation of the transfer factors. The transfer factor solution can improve the disease-resisting capability of organisms, relieve immunologic suppression and immunologic tolerance, can play a role in emergency adjuvant treatment, and makes up for immunization blank after vaccination before antibody production.
Description
Technical field
The invention belongs to a kind of transfer factor that is used for improving immunity of livestock in the technical field of bioengineering, particularly a kind of in the transfer factor solution used on the poultry and preparation method thereof and the application on poultry.
Background technology
Along with improving constantly of scale, intensification degree cultured in modernization, viral disease has become puzzlement raiser's a great problem at present.Existing general solution is exactly immunity, yet for various reasons, sometimes can cause immuning failure unavoidably, break out atypical infectious disease, bring certain difficulty behind immuning failure, often to rely on antibiotic therapy for diagnosis and treatment, caused the increase of Resistant strain and the generation of cross infection.Transfer factor can play an important role aspect disease preventing and treating as a kind of cellular immunization reinforcing agent, improves the immune effect after the immunity, effectively improves the resistance against diseases of body.Present existing transfer factor solution technology of preparing, extract again after normally the fat in the spleen of animal and trunk being picked out, carry out a series of programs such as breaking cellular wall freeze thawing dialysis, though the method is simple, but often need great amount of manpower and material resources, and the transfer factor solution that this kind method is prepared can only be preserved under-15 ℃ condition, to using and transporting and all brought very big inconvenience.
Summary of the invention
Technical problem to be solved by this invention is: a kind of transfer factor solution of using on poultry and preparation method thereof is provided.Effectively improve above-mentioned situation, consolidate the immune effect behind the fowl poultry immune, make transfer factor in animal husbandry, be able to extensive use.In order to improve the output capacity of transfer factor solution, the present invention adopts pig spleen to extract transfer factor, and the extraction process of traditional transfer factor is compared optimization, designs the low transfer factor preparation of the high production cost of the rate of output.
Technical scheme of the present invention:
A kind of transfer factor solution of on poultry, using, it is characterized in that: be to adopt following method preparation: with refrigerated pig spleen 500g, be cut into small pieces after cleaning with distilled water, adding 1000ml does not have thermal source matter distilled water, use tissue mashing machine's smudge cells, make homogenate behind the mixing., add flocculating agent and flocculate by behind the high pressure homogenizer with 60 kPas pressure.Homogenate after the flocculation is centrifugal, 4 ℃ of Centrifugal Environment temperature, 7000r/min removes precipitation, stays supernatant standby.Supernatant filters with the microporous filter membrane of 0.45 μ m, is that 5000 dalton's ultrafilter membranes carry out ultrafiltration with molecular cut off then, and the filtrate of gained is transfer factor stock solution.Stock solution is removed the preparation of virus back according to the national drug management method.The nicotiamide 2ml that adds concentration range during preparation and be 1.25%-2.5% filters back adding 5g glycerol as heat resisting protective as stabilizing agent with 0.22 μ m microporous filter membrane aseptic filtration, and direct packaging promptly obtains transfer factor solution.
The preparation method of the transfer factor solution that the present invention uses on poultry is characterized in that: with refrigerated pig spleen 500g, be cut into small pieces after cleaning with distilled water, adding 1000ml does not have thermal source matter distilled water, uses tissue mashing machine's smudge cells, makes homogenate behind the mixing., add flocculating agent and flocculate by behind the high pressure homogenizer with 60 kPas pressure.Homogenate after the flocculation is centrifugal, 4 ℃ of Centrifugal Environment temperature, 7000r/min removes precipitation, stays supernatant standby.Supernatant filters with the microporous filter membrane of 0.45 μ m, is that 5000 dalton's ultrafilter membranes carry out ultrafiltration with molecular cut off then, and the filtrate of gained is transfer factor stock solution.Stock solution is removed the preparation of virus back according to the national drug management method.The nicotiamide 2ml that adds concentration range during preparation and be 1.25%-2.5% filters back adding 5g glycerol as heat resisting protective as stabilizing agent with 0.22 μ m microporous filter membrane aseptic filtration, and direct packaging promptly obtains transfer factor solution.
The transfer factor solution of the present invention for using on poultry is characterized in that: can be used treatment antibacterial and viral mixed infection with vaccine; Consumption: poultry: mix drink, below 30 ages in days, 0.1-0.2 milliliter/plumage; More than 30 ages in days, 0.5 milliliter/plumage.
Beneficial effect of the present invention:
The present invention improves existing transfer factor technology; directly extract with pig spleen; after spleen adopted tissue mashing machine's breaking cellular wall; carry out further breaking cellular wall by high pressure homogenizer; the flocculation back is centrifugal; carry out ultrafiltration at last; great amount of manpower and material resources have been saved; effectively raising extraction ratio combines the heat-resisting protective technology simultaneously with transfer factor; make the storage temperature of transfer factor bring up to room temperature by-15 ℃; realized the room temperature preservation of transfer factor; this technology had both adapted to the animal husbandry demand of practical production; make transfer factor solution need not keep low temperature environment again in transportation with in using; transfer factor solution has improved body resistance against diseases as a kind of cell immune response reinforcing agent simultaneously, has removed the immunosuppressant and the immunologic tolerance of body; to the effect that ill drove can produce urgent auxiliary treatment, the immunity that has also remedied before antibody produces after the vaccination is blank.
The specific embodiment:
The preparation method of the transfer factor solution that the present invention uses on poultry with refrigerated pig spleen 500g, is cut into small pieces after cleaning with distilled water, and adding 1000ml does not have thermal source matter distilled water, uses tissue mashing machine's smudge cells, makes homogenate behind the mixing., add flocculating agent and flocculate by behind the high pressure homogenizer with 60 kPas pressure.Homogenate after the flocculation is centrifugal, 4 ℃ of Centrifugal Environment temperature, 7000r/min removes precipitation, stays supernatant standby.Supernatant filters with the microporous filter membrane of 0.45 μ m, is that 5000 dalton's ultrafilter membranes carry out ultrafiltration with molecular cut off then, and the filtrate of gained is transfer factor stock solution.Stock solution is removed the preparation of virus back according to the national drug management method.The nicotiamide 2ml that adds concentration range during preparation and be 1.25%-2.5% filters back adding 5g glycerol as heat resisting protective as stabilizing agent with 0.22 μ m microporous filter membrane aseptic filtration, and direct packaging promptly obtains transfer factor solution.
The application of this transfer factor solution on poultry: can be used to consolidate vaccine effect with vaccine, the immunity that remedies before antibody produces after the vaccination is blank; Also can be used for treating poultry antibacterial and the viral mixed infectious disease that causes.
Claims (3)
1, with refrigerated pig spleen 500g, be cut into small pieces after cleaning with distilled water, adding 1000ml does not have thermal source matter distilled water, uses tissue mashing machine's smudge cells, makes homogenate behind the mixing., add flocculating agent and flocculate by behind the high pressure homogenizer with 60 kPas pressure.Homogenate after the flocculation is centrifugal, 4 ℃ of Centrifugal Environment temperature, 7000r/min removes precipitation, stays supernatant standby.Supernatant filters with the microporous filter membrane of 0.45 μ m, is that 5000 dalton's ultrafilter membranes carry out ultrafiltration with molecular cut off then, and the filtrate of gained is transfer factor stock solution.Stock solution is removed the preparation of virus back according to the national drug management method.The nicotiamide 2ml that adds concentration range during preparation and be 1.25%-2.5% is as stabilizing agent, with 0.22 μ m microporous filter membrane aseptic filtration.Filter the back and add 5g glycerol as heat resisting protective, direct packaging promptly obtains transfer factor solution.。
2, the preparation method of the transfer factor solution of on poultry, using according to claim 1, with refrigerated pig spleen 500g, be cut into small pieces after cleaning with distilled water, adding 1000ml does not have thermal source matter distilled water, use tissue mashing machine's smudge cells, make homogenate behind the mixing., add flocculating agent and flocculate by behind the high pressure homogenizer with 60 kPas pressure.Homogenate after the flocculation is centrifugal, 4 ℃ of Centrifugal Environment temperature, 7000r/min removes precipitation, stays supernatant standby.Supernatant filters with the microporous filter membrane of 0.45 μ m, is that 5000 dalton's ultrafilter membranes carry out ultrafiltration with molecular cut off then, and the filtrate of gained is transfer factor stock solution.Stock solution is removed the preparation of virus back according to the national drug management method.The nicotiamide 2ml that adds concentration range during preparation and be 1.25%-2.5% is as stabilizing agent, with 0.22 μ m microporous filter membrane aseptic filtration.Filter the back and add 5g glycerol as heat resisting protective, direct packaging promptly obtains transfer factor solution.。
3, the transfer factor solution of using on poultry according to claim 1 is characterized in that: can be used to consolidate vaccine effect with vaccine, the immunity that remedies before antibody produces after the vaccination is blank; Also can be used for treating poultry antibacterial and the viral mixed infectious disease that causes.。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810052451A CN101537169A (en) | 2008-03-17 | 2008-03-17 | Transfer factor solution for livestock and poultry, and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810052451A CN101537169A (en) | 2008-03-17 | 2008-03-17 | Transfer factor solution for livestock and poultry, and preparation method thereof |
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| CN101537169A true CN101537169A (en) | 2009-09-23 |
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| CN200810052451A Pending CN101537169A (en) | 2008-03-17 | 2008-03-17 | Transfer factor solution for livestock and poultry, and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102389036A (en) * | 2011-10-13 | 2012-03-28 | 山东省健牧生物药业有限公司 | Macroergic multivitamin-amino acid emulsion and preparation method thereof |
| CN102397293A (en) * | 2010-09-19 | 2012-04-04 | 曹吉祥 | Spleen immune factor and its preparation method |
| CN102475718A (en) * | 2010-11-23 | 2012-05-30 | 赵满达 | Transfer factor soluble powder for poultry, as well as preparation process and quality inspection method thereof |
| CN103316045A (en) * | 2013-06-26 | 2013-09-25 | 西安斯凯达生物制品有限公司 | Combined specific transfer factor for livestock and poultry and preparation method of combined specific transfer factor |
| CN103724420A (en) * | 2013-12-30 | 2014-04-16 | 天津瑞普生物技术股份有限公司 | Pig spleen transfer factor extracting method |
| CN103892166A (en) * | 2014-04-11 | 2014-07-02 | 江西德泰医药生物技术有限公司 | Method for preparing health food by polypeptide factor and Chinese herb extracts |
| CN104208678A (en) * | 2013-09-30 | 2014-12-17 | 郑州后羿制药有限公司 | Veterinary antivirus composition, freeze-dried powder, preparation method and applications of the composition |
-
2008
- 2008-03-17 CN CN200810052451A patent/CN101537169A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102397293A (en) * | 2010-09-19 | 2012-04-04 | 曹吉祥 | Spleen immune factor and its preparation method |
| CN102475718A (en) * | 2010-11-23 | 2012-05-30 | 赵满达 | Transfer factor soluble powder for poultry, as well as preparation process and quality inspection method thereof |
| CN102389036A (en) * | 2011-10-13 | 2012-03-28 | 山东省健牧生物药业有限公司 | Macroergic multivitamin-amino acid emulsion and preparation method thereof |
| CN102389036B (en) * | 2011-10-13 | 2012-12-05 | 山东省健牧生物药业有限公司 | Macroergic multivitamin-amino acid emulsion and preparation method thereof |
| CN103316045A (en) * | 2013-06-26 | 2013-09-25 | 西安斯凯达生物制品有限公司 | Combined specific transfer factor for livestock and poultry and preparation method of combined specific transfer factor |
| CN103316045B (en) * | 2013-06-26 | 2015-08-12 | 西安斯凯达生物制品有限公司 | A kind of livestock and poultry multi-joint specific transfer factor and preparation method |
| CN104208678A (en) * | 2013-09-30 | 2014-12-17 | 郑州后羿制药有限公司 | Veterinary antivirus composition, freeze-dried powder, preparation method and applications of the composition |
| CN104208678B (en) * | 2013-09-30 | 2016-04-20 | 郑州后羿制药有限公司 | A kind of antivirus veterinary composition, lyophilized powder, preparation method and application |
| CN103724420A (en) * | 2013-12-30 | 2014-04-16 | 天津瑞普生物技术股份有限公司 | Pig spleen transfer factor extracting method |
| CN103724420B (en) * | 2013-12-30 | 2016-02-24 | 天津瑞普生物技术股份有限公司 | A kind of Swine spleen transfer factor extracting method |
| CN103892166A (en) * | 2014-04-11 | 2014-07-02 | 江西德泰医药生物技术有限公司 | Method for preparing health food by polypeptide factor and Chinese herb extracts |
| CN103892166B (en) * | 2014-04-11 | 2016-11-23 | 江西德泰医药生物技术有限公司 | A kind of polypeptide factor and Chinese herbal medicine extract prepare the method for health food |
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Open date: 20090923 |