CN101507766B - Preparation method of traditional Chinese medicine capsules with blood-fat reduction and health-care function and use thereof - Google Patents
Preparation method of traditional Chinese medicine capsules with blood-fat reduction and health-care function and use thereof Download PDFInfo
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Abstract
The invention relates to a method for preparing Chinese medicinal capsules with the functions of hypolipemic and health care and application of the Chinese medicinal capsules. The method comprises the following steps: mixing and stirring an extracted prinsepia utilis royle oil active composition, an extracted hawkthorn effective composition and vitamin E in a mixer; drying the mixture at a temperature of between 50 and 70 DEG C until the moisture is less than 3 to 5 percent; taking out the obtained product, crushing the obtained product until the granularity is between 60 and 80 meshes; and performing encapsulation to prepare soft capsules. The method for preparing microcapsules comprises the following steps: firstly, adding 10 percent gelatin aqueous solution and sucrose ester emulsion into raw materials of the Chinese medicinal capsules in a reactor, stirring the mixture, and heating the mixture to the temperature of 46 DEG C; secondly, adding 10 percent arabic gum aqueous solution into the mixture, adjusting the pH value of the obtained solution to be 4.5, stirring the obtained solution, and cooling the obtained solution to the temperature of 24 DEG C; thirdly, using 10 percent sodium carbonate to adjust the pH value of the obtained solution to be 8.0, and stirring the obtained solution; fourthly, adding diisopropyl phosphate and 30 percent formaldehyde solution into the obtained solution; and fifthly, keeping the obtained solution stand, removing supernatant, using water to wash microspheres until the microspheres are nearly neutral, and drying and packaging the microspheres. The Chinese medicinal capsules have the efficacies of reducing blood fat, preventing fatty liver, protecting the liver, and the like.
Description
Technical field
The present invention relates to a kind of preparation method and its usage of the Chinese medicinal capsule of making by Princepia utilis oil active component and Fructus Crataegi effective ingredient and vitamin E with blood fat reducing and health care.
Background technology
Hyperlipidemia is meant that VLDL in the blood, LDL increase, and plasma cholesterol is higher than 230mg/100ml and triglyceride is higher than 140mg/100ml.In the modern life, hyperlipidemia, fatty liver have become a kind of commonly encountered diseases of middle-aged and elderly people, and along with growth in the living standard, sickness rate has obvious ascendant trend, and the trend of rejuvenation is arranged.Blood lipid metabolism imbalance, endocrine regulation, alcoholic liver injury, drinking and eating irregularly etc. are the main causes that causes hyperlipidemia, fatty liver.
Great mass of data shows that hyperlipidemia is apoplexy, coronary heart disease, myocardial infarction, the heart soldier is independently dead and important risk.In addition, hyperlipidemia also is an important risk factor that promotes hypertension, impaired glucose tolerance, diabetes.Hyperlipidemia also can cause fatty liver, liver cirrhosis, cholelithiasis, pancreatitis, retinal hemorrhage, blind, peripheral vascular disease, lame property, hyperuricemia etc.Therefore prevention and treatment hyperlipidemia seem particularly important.
Hyperlipidemia has the characteristics of chronicity in its prevention and treatment, and the Western medicine that adopts clinically at present can produce many untoward reaction and weak curative effect and toxic side effect in taking for a long time.Therefore, eat homologous viewpoint according to theory of Chinese medical science Chinese medicine, utilize and contain multiple accent fat active component in the Chinese medicine, act on a plurality of target spots and produce comprehensive therapeutic effect, action temperature and lasting, and to characteristics such as Liver and kidney toxicity are little, research and development are the most important things of present blood lipid-lowering medicine research with the green natural health preparation of blood fat reducing, anti-fatty liver, anti-hepatic fibrosis, safe without toxic side effect.
Princepia utilis (Prinsepia veilis Ro Yie) is a Rosaceae Prinsepia Royle plant, is born in the limit, field, ditch, hillside, wasteland, jungle of height above sea level 1800-3200m, is distributed in northwestern Yunnan Province, and the Lijiang Prefecture is maximum.The high 1-2m of Princepia utilis plant has the green grass or young crops that grows firmly to sting because of long, often by peasant household's plantation exclosure.The Princepia utilis leaf is elongated, and early promptly bloom floral white around the Spring Festival florescence.Fruit phase 5-6 month, thousand grain weigth 116.8g.
Princepia utilis oil is an oils and fats part contained in its fruit, is China's shuttle clansman ticbit and edible oil always that rub, and among the peoplely thinks that it is a kind ofly to control the medicine of all kinds of diseases and ailments and be used for multiple treatment of diseases.The present invention finds the composed as follows of Princepia utilis oil: Palmic acid 22.56% by gas-matter coupling check and analysis; Stearic acid 5.09%; Oleic acid 33.44%; Linoleic acid 37.02%; Linolenic acid 1.45%; Caproic acid 0.44%.As seen wherein contain abundant oleic acid (33.44%) and linoleic acid (37.02%).And the effect metabolism of unsaturatedization of linoleic acid process enzyme is γ one linolenic acid, γ one linolenic acid is the arachidonic acid except continuing metabolism, itself also has certain physiological function, is the effect of cholesterol regulating as the symptom of alleviating dysmenorrhea such as Ah appropriate's dermatitis, gout.Arachidonic acid also has following physiological function except changing various prostaglandins into: protection gastric mucosa, treatment dry skin tinea disease, prevention fatty liver, effects such as the liver protecting and killing cancer cell.The effect that linolenic acid prolongs enzyme by unsaturatedization enzyme and carbochain, metabolism is eicosapentaenoic acid (EPA), metabolism is docosahexenoic acid (DHA) then.EPA has blood fat reducing and platelet consolidation, thus can prevention of brain blood fasten and disease such as myocardial infarction.DHA has the neural growth of promotion, improving memory and retinal reflex ability, prevention infirmities of age and cancer, effects such as agaist allergic symptoms. in addition, Princepia utilis oil also contains abundant vitamin: 3-Hydroxyretinol .5mg/100g, vitamin D2 .1mg/100g, vitamin e1 1.2mg/100g and vitamin K 0.3mg/100g.
Princepia utilis is grown in height above sea level than higher and free of contamination area; thereby be a kind of green plants; and have above-mentioned blood fat reducing, alleviate Ah appropriate's dermatitis, gout, protection gastric mucosa; treatment dry skin tinea disease; the prevention fatty liver; the liver protecting and killing cancer cell, prevention of brain blood are fastened and disease, improving memory and retinal reflex ability such as myocardial infarction, prevention infirmities of age and cancer, effects such as agaist allergic symptoms.Often edible Princepia utilis oil, but blood fat reducing, blood pressure regulation promotes microcirculation, and the resistance of enhancing body makes people's life lengthening, and Princepia utilis oil also is widely used in treatment skin burn, skin ulceration etc.Thereby become at present a kind of plant with important medical treatment and health care value, be known as " oil plant gold " by the oiliness composition that squeezes in its kernel.
Fructus Crataegi: middle Fructus Crataegi has the effect of blood circulation promoting and blood stasis dispelling, it is again the key medicine of greasy meat stagnation of disappearing, " Chinese medicine information " (2000 (6), 29-30) in to its Pharmacological action study, report, its chemical constituent is mainly glycoside organic acid, flavonoid, terpenoid, the effect that has blood fat reducing, blood pressure lowering and invigorate blood circulation.Modern pharmacological research proof Fructus Crataegi can make the lecithin in the blood raise, and cholesterol and the lipid deposition on organ is reduced, and its extract can reduce the serum cholesterol (TC) and the triglyceride (TG) of mouse model.
Vitamin E has removing interior free yl (SOD) and function in delaying senility.
Summary of the invention
The purpose of this invention is to provide and a kind ofly derive from edible natural material and have blood fat reducing and the capsule of health care and its production and use.
Gross weight in effective ingredient is 20 parts, and the components by weight percent of each effective ingredient is in the soft capsule:
Princepia utilis oil active component: 10-18
Fructus Crataegi effective ingredient: 1.7-7
Vitamin E: 0.3-3
Preferred raw material weight proportioning is:
Princepia utilis oil active component: 14
Fructus Crataegi effective ingredient: 5
Vitamin E: 1
The present invention also provides the method for the preparation of soft capsule with blood fat reducing and health care:
The preparation method of Princepia utilis oil: adopt oil expression method commonly used at present: will clear up and shine the Princepia utilis raw material after coarse powder is removed the peel, is ground into to dry doubling, steaming before the oil expression is fried and is handled after 1-3 hour in the usual way, squeeze and collect the oils and fats of squeezing out with spiral crusher, to its Residual oil≤10%.The solid shape precipitate of filtering promptly obtains operational limpid oils and fats.During preparation, Princepia utilis is controlled at and makes its water content is that 8-10% and temperature are to steam under 85-115 ℃ the condition to fry to handle, and can obtain comparatively satisfied expressed oil rate.
The preparation method of Princepia utilis oil active component: the active component that has effect for reducing blood fat in the Princepia utilis oil mainly is a unsaturated fatty acid, separates two kinds of methods of unsaturated fatty acid at low temperatures with solvent method and prepares the Princepia utilis oil active component so can take the carbamide bag to separate unsaturated fatty acid with method.
Method one: carbamide bag and legal system are equipped with the Princepia utilis oil active component;
The 15g potassium hydroxide is dissolved in 420ml 95% ethanol, adds the 60g Princepia utilis oil, and in alcohol reflux temperature saponification 45min, stirring makes and reacts completely.The stirring saponification liquor makes and is cooled to 5 ℃, keeps 60min under this temperature, and sucking filtration gets transparent filtrate, concentrates to reclaim ethanol, makes ethanol content be not more than 50%, and concentrated solution adds the 200ml distilled water, with hydrochloric acid accent PH to 2, and separation upper strata fluid.70 ℃ of water-bath preparations contain the alcoholic solution 480ml of certain proportion carbamide, add above gained oil reservoir, 70 ℃ of stirring in water bath make solution be uniform transparent phase, be cooled to 5 ℃, place 6h under this temperature, the clathrate that removes by filter formation concentrates and reclaims ethanol, add the 250ml distilled water diluting, use the 200ml chloroform extraction, reclaim chloroform, obtain spissated polyunsaturated fatty acid.
Method two: solvent method prepares the Princepia utilis oil active component at low temperatures;
With normal hexane and Princepia utilis mixed by 5: 2, air-tight state is in-5--10 ℃ refrigerator-freezer in freezing 2-24 hour, satisfied fatty acid is crystalline state and separates out, both are separated, wash satisfied fatty acid repeatedly with normal hexane simultaneously, last concentrating under reduced pressure normal hexane obtains unsaturated fatty acid.
The preparation method of Fructus Crataegi effective ingredient: choose Fructus Crataegi, clear water is cleaned, chopping, the ethanol of the 80-95% that adding 3-10 doubly measures, be heated to 50-80 ℃, reflux, extract, 2-5 time, each extraction time is 2-5 hour, and extracting solution 100 orders are crossed screen cloth, merge ethanol filtrate, decompression recycling ethanol, concentrated solution 100 orders are crossed screen cloth, with macroporous resin column on the filterable concentrated solution, water successively, the 60-80% ethanol elution, collect the 60-80% ethanol elution, decompression recycling ethanol continues to concentrate, cross 100 orders, with the concentrated solution spray drying, collect spray powder, promptly make the Fructus Crataegi effective ingredient.
The preparation method of preferred Fructus Crataegi effective ingredient: Fructus Crataegi, clear water is cleaned, chopping, put in the multi-function extractor, add 80% ethanol of 7 times of amounts, be heated to 60 ℃, reflux, extract, 3 times, each extraction time is 4 hours, extracting solution 100 orders are crossed screen cloth, merge ethanol filtrate ,-80kPa, 60 ℃ of decompression recycling ethanols, continue to be concentrated into the concentrated solution of 1/2 volume (V/W) of Fructus Crataegi inventory, 100 orders are crossed screen cloth, with macroporous resin column on the filterable concentrated solution, water successively, 60% ethanol elution, collect 60% ethanol elution ,-80kPa, 60 ℃ of decompression recycling ethanols, continue to concentrate, 60 ℃ of hot down survey relative densities are 1.10-1.13, cross 100 orders, with the concentrated solution spray drying, collect spray powder, promptly make the Fructus Crataegi effective ingredient.
Preparation with Chinese medicinal soft capsule of blood fat reducing and health care: by proportioning raw materials of the present invention, component is Princepia utilis oil active component: 10-18 by weight; Fructus Crataegi effective ingredient: 1.7-7; Vitamin E: the 0.3-3 ratio is weighed, and places in the mixer and mixes, and stirs, and moves into baking oven, and 50-70 ℃ is dried by the fire to moisture and be lower than 3-5%, takes out, and is crushed to the 60-80 order, jar capsule.
Optimum with effective each composition of principal agent of natural Chinese medicine of blood fat reducing and health care is joined:
Princepia utilis oil active component: 14
Fructus Crataegi effective ingredient: 5
Vitamin E: 1
Preparation with Chinese medicine microcapsule capsule of blood fat reducing and health care:
Described microcapsule is to be capsule-core with raw material of the present invention, accounts for the 30-90% weight ratio, is softgel shell with gelatin and Radix Acaciae senegalis, softgel shell accounts for 5-30%, and allocate emulsifying agent, fixative, dispersant, PH regulator into, by existing complex coacervation, make microcapsule.Each composition weight proportion is: the content of proportioning raw materials of the present invention is 30-90%, gelatin and Radix Acaciae senegalis content are 3-30%, emulsifier content is 2-40%, curing agent content is 3-45%, dispersant is 0.1-10%, it is 0.01-0.5% that PH regulates dosage, and each component sum is 100%.
Emulsifying agent can be selected any one or more in sucrose ester, spans, Tweens and the castor oil derivatives etc. for use; Firming agent can be selected the formalin of 10-37% for use; Dispersant can be selected any one or more in alkylsulfonate, the diisopropyl phosphate for use; The PH regulator can be selected sodium hydroxide for use, in oxyammonia or the acetic acid any.
The optimal proportion of each component content is in the microcapsule:
Proportioning raw materials content of the present invention is 35-70%,
Gelatin and Radix Acaciae senegalis content are 10-30%, and emulsifier content is 5-15%,
Curing agent content is 10-20%, and dispersant is 1-5%,
It is 0.05-0.2% that PH regulates dosage, and each component sum is 100%.
The present invention has following advantage: safe and effective, have health care, prevention and therapeutical effect concurrently.Its concrete effect is as follows: the present invention has blood fat reducing, prevention fatty liver, and effects such as the liver protecting are often edible, but blood fat reducing, blood pressure regulation promotes microcirculation, the resistance of enhancing body makes people's life lengthening.
The specific embodiment
Embodiment 1
Chinese medicinal soft capsule with blood fat reducing and health care: by proportioning raw materials of the present invention, component is 17 parts of Princepia utilis oil active component by weight; 1.5 parts of Fructus Crataegi effective ingredient; Vitamin e1 .5 part ratio is weighed, and places in the mixer and mixes, and stirs, and moves into baking oven, and 50 ℃ are dried by the fire to moisture and are lower than 3%, take out, and are crushed to 80 orders, jar capsule.
30% Chinese medicine microcapsule with blood fat reducing and health care:
In reactor, 30g proportioning raw materials of the present invention is added in the aqueous gelatin solution of 25g 10%, add sucrose ester emulsion 6g simultaneously, stir, be heated to 46 ℃, add the arabic gum aqueous solution of 25g10% then, adjusting pH value is 4.1, stir, be cooled to below 8 ℃, reuse 10% sodium carbonate is transferred PH8.4, stirs 15min, the formalin that adds 4g diisopropyl phosphate and 10g30% at last stirs 1h under this temperature.Leave standstill, the tipping supernatant washes microsphere with water near neutral, drying for standby.
Embodiment 2
Chinese medicinal soft capsule with blood fat reducing and health care: by proportioning raw materials of the present invention, by weight component be Princepia utilis oil or extract after 15 parts of Princepia utilis oil active component; 2.5 parts of Fructus Crataegis after Fructus Crataegi or the extraction; Vitamin E2 .5 part ratio is weighed, and places in the mixer and mixes, and stirs, and moves into baking oven, and 60 ℃ are dried by the fire to moisture and are lower than 4%, take out, and are crushed to 70 orders, jar capsule.
50% Chinese medicine microcapsule with blood fat reducing and health care:
In reactor, 50g proportioning raw materials of the present invention is added in the aqueous gelatin solution of 25g 10%, add sucrose ester emulsion 6g simultaneously, stir, be heated to 45 ℃, add the arabic gum aqueous solution of 25g10% then, adjusting pH value is 4.3, stir, be cooled to below 8 ℃, reuse 10% sodium carbonate is transferred PH8.8, stirs 15min, the formalin that adds 4g diisopropyl phosphate and 10g30% at last stirs 1h under this temperature.Leave standstill, the tipping supernatant washes microsphere with water near neutral, drying for standby.
Embodiment 3:
Chinese medicinal soft capsule with blood fat reducing and health care: by proportioning raw materials of the present invention, by weight component be Princepia utilis oil or extract after 18 parts of Princepia utilis oil active component; 1.5 parts of Fructus Crataegis after Fructus Crataegi or the extraction; 0.5 part of ratio of vitamin E is weighed, and places in the mixer and mixes, and stirs, and moves into baking oven, and 70 ℃ are dried by the fire to moisture and are lower than 3%, take out, and are crushed to 70 orders, jar capsule.
70% Chinese medicine microcapsule with blood fat reducing and health care:
In reactor, 70g proportioning raw materials of the present invention is added in the aqueous gelatin solution of 25g 10%, add sucrose ester emulsion 6g simultaneously, stir, be heated to 46 ℃, add the arabic gum aqueous solution of 25g10% then, adjusting pH value is 4.5, stir, be cooled to below 8 ℃, reuse 10% sodium carbonate is transferred PH8.2, stirs 15min, the formalin that adds 4g diisopropyl phosphate and 10g30% at last stirs 1h under this temperature.Leave standstill, the tipping supernatant washes microsphere with water near neutral, drying for standby.
Embodiment 4
Chinese medicinal soft capsule with blood fat reducing and health care: by proportioning raw materials of the present invention, by weight component be Princepia utilis oil or extract after 14 parts of Princepia utilis oil active component; 5 parts of Fructus Crataegis after Fructus Crataegi or the extraction; Vitamin e1 part ratio is weighed, and places in the mixer and mixes, and stirs, and moves into baking oven, and 80 ℃ are dried by the fire to moisture and are lower than 3%, take out, and are crushed to 80 orders, jar capsule.
90% Chinese medicine microcapsule with blood fat reducing and health care:
In reactor, 90g proportioning raw materials of the present invention is added in the aqueous gelatin solution of 25g 10%, add sucrose ester emulsion 6g simultaneously, stir, be heated to 46 ℃, add the arabic gum aqueous solution of 25g10% then, adjusting pH value is 4.5, stir, be cooled to below 24 ℃, reuse 10% sodium carbonate is transferred PH8.0, stirs 15min, the formalin that adds 4g diisopropyl phosphate and 10g30% at last stirs 1h under this temperature.Leave standstill, the tipping supernatant washes microsphere with water near neutral, drying for standby.
The toxicology test of product and effect test:
1 toxicologic study shows:
1) acute toxicity: the blood fat reducing capsule all greater than the 20g/kg body weight, belongs to non-toxic type to rat, its mouse oral LD50 male and female.
2) micronucleus test: the blood fat reducing capsule is negative to PCEMNR micronucleus test testing result.
3) mouse sperm deformity test: the blood fat reducing capsule is negative to mouse sperm deformity test testing result.
2 lipid-lowering tests:
Experimental animal is equally divided into five groups:
I group: positive controls, the high lipid food of feeding;
II group: when feeding high lipid food, irritate stomach preparation 250mg/kg of the present invention every day;
III group: when feeding high lipid food, irritate stomach preparation 500mg/kg of the present invention every day;
IV group: when feeding high lipid food, irritate stomach preparation 1000mg/kg of the present invention every day;
The V group: negative control group, the normal diet of feeding is irritated stomach every day with the volume excipient; 30 days laggard promoting the circulation of blood fat of jar stomach is measured continuously.Result of the test sees Table 1
Table 1 has the Chinese medicinal capsule of blood fat reducing and health care to the influence of high fat mice TC and TG (x-± S)
| Group | TC(mmol/l) | TG(mmol/l) |
| Negative control group | 2.29±0.22 | 0.93±0.33 |
| High fat group | 4.27±0.31▲▲ | 1.38±0.07▲▲ |
| Low dose group | 2.32±0.07** | 0.96±0.05** |
| Middle dosage group | 2.56±1.08 | 1.33±0.28* |
| High dose group | 3.22±0.60* | 1.39±0.35* |
Compare with high fat matched group: * P＜0.05, compare with the normal control group * * P＜0.01: ▲ P＜0.05, ▲ ▲ P＜0.01
The result shows: the high lipid food group is compared with the normal control group, and serum total cholesterol (TC) and triglyceride (TG) all significantly increase, and shows to make the high blood lipid model success of the test; And the test group of irritating stomach various dose preparation is compared with IUGR high-fat diet group, and serum total cholesterol (TC) and triglyceride (TG) all reduce, and wherein low dose group (250mg/kg) reduces the most remarkable.
3 preparations of the present invention cause that to hyperlipidemia liver function causes the protective effect of damage
Experimental animal grouping and processing are tested with blood fat reducing, after the off-test with the experimental animal sacrificed by decapitation, get blood and separation of serum, the level of glutamate pyruvate transaminase (ALT) glutamic oxaloacetic transaminase, GOT (AST) r-glutamyl transpeptidase (GGT) and alkali phosphatase (ALP) in the mensuration serum.
The result shows that the hyperlipidemia group is compared with negative control group, and glutamic oxaloacetic transaminase, GOT significantly raises, and after jar stomach preparation various dose of the present invention, can significantly reduce the rising of glutamic oxaloacetic transaminase, GOT; Glutamate pyruvate transaminase there is not influence; R-GT transaminase and alkali phosphatase also have the effect of reduction, and visible preparation of the present invention has protective effect to the hepatic injury that hyperlipidemia causes.
Experimental result sees Table 2 and table 3
Table 2: preparation of the present invention causes the protective effect of liver function damage to hyperlipidemia
| Group | Glutamate pyruvate transaminase | Glutamic oxaloacetic transaminase, GOT | Transaminase's ratio | The r-GT transaminase | Alkali phosphorus enzyme |
| Dosage group high dose group in the high fat group of the negative control group low dose group | 56.5±14.27 79±8.49 64.5±25.68 72.5±69.11 81.7±40.33 | 285.7±75.94 529±52.33** 169.2±18.28 ★★ 203.2±69.33 ★★ 251±98.42 ★ | 5.1±1.04 6.7±0.06 2.93±1.02 ★★ 4.35±2.87 3.34±1.44 | 10±5.10 12.5±2.12 6±2.16 ★ 4±2.45 ★ 7±3.16 | 186.75±45.47 208±9.89 230.5±97.72 166.75±13.20 ★ 144.75±21.34 ★ |
Compare with matched group: * 0.01＜P＜0.05, compare with high fat group * * P＜0.01: ★ 0.01＜P＜0.05, ★ ★ P＜0.01
Table 3: preparation of the present invention causes the protective effect of liver function damage to hyperlipidemia
| Group | Negative control group | High fat group | Low dose group | Middle dosage group | High dose group |
| Total protein content albumin content globulin content Archon is than total bilirubin bilirubin direct unconjugated bilirubin carbamide | 59±4.32 34.25±2.22 24.75±3.59 1.41±0.24 1.15±0.19 0.25±0.13 0.9±0.18 8.3±2.04 | 59.5±2.12 30.5±2.12 29±0.2 1.05±0.07 1.6±0.28 0.35±0.07 1.25±0.35 10.15±0.49 | 61.75±2.75 32.75±2.87 29±3.56 1.13±0.19 1.28±0.43 0.58±0.24 0.7±0.2 9.83±5.10 | 55±4.32 31±1.15 ★ 24±3.83 1.32±0.21 2.28±0.66* 0.8±0.18** 1.48±0.49 6.3±1.73 | 63..25±4.72 34.25±2.22 29±3.37 1.19±0.14 1.85±0.26** 0.58±0.13* 1.3±0.35 5±0.75 ★★ |
Compare with matched group: * 0.01＜P＜0.05, compare with high fat group * * P＜0.01: ★ 0.01＜P＜0.05, ★ ★ P＜0.01
Claims (4)
1. the preparation method with Chinese medicinal soft capsule of blood fat reducing and health care is characterized in that component is Princepia utilis oil active component: 10-18 by weight; Fructus Crataegi effective ingredient: 1.7-7; Vitamin E: the 0.3-3 ratio is weighed, and places in the mixer and mixes, and stirs, and moves into baking oven, and 50-70 ℃ is dried by the fire to moisture and be lower than 3-5%, takes out, and is crushed to the 60-80 order, jar capsule;
The preparation method of described Princepia utilis oil active component is: will clear up and shine the Princepia utilis raw material after coarse powder is removed the peel, is ground into to dry doubling, steaming before the oil expression is fried and is handled after 1-3 hour in the usual way, squeeze and collect the oils and fats of squeezing out with spiral crusher, to its Residual oil≤10%, the solid shape precipitate of filtering promptly obtains operational limpid Princepia utilis oil; During preparation, Princepia utilis is controlled at and makes its water content is that 8-10% and temperature are to steam under 85-115 ℃ the condition to fry to handle; Get the 15g potassium hydroxide and be dissolved in 420ml 95% ethanol, add above-mentioned Princepia utilis oil 60g, in alcohol reflux temperature saponification 45min, stirring makes and reacts completely, the stirring saponification liquor makes and is cooled to 5 ℃, keeps 60min under this temperature, sucking filtration, get transparent filtrate, concentrate and reclaim ethanol, make ethanol content be not more than 50%, concentrated solution adds the 200ml distilled water, transfer PH to 2 with hydrochloric acid, separate upper strata fluid; 70 ℃ of water-bath preparations contain the alcoholic solution 480ml of certain proportion carbamide, add above gained oil reservoir, 70 ℃ of stirring in water bath make solution be uniform transparent phase, be cooled to 5 ℃, place 6h under this temperature, the clathrate that removes by filter formation concentrates and reclaims ethanol, add the 250ml distilled water diluting, use the 200ml chloroform extraction, reclaim chloroform, obtain spissated Princepia utilis oil active component unsaturated fatty acid;
The preparation method of described Fructus Crataegi effective ingredient is: choose Fructus Crataegi, clear water is cleaned, chopping, the ethanol of the 80-95% that adding 3-10 doubly measures, be heated to 50-80 ℃, reflux, extract, 2-5 time, each extraction time is 2-5 hour, and extracting solution 100 orders are crossed screen cloth, merge ethanol filtrate, decompression recycling ethanol, concentrated solution 100 orders are crossed screen cloth, with macroporous resin column on the filterable concentrated solution, water successively, the 60-80% ethanol elution, collect the 60-80% ethanol elution, decompression recycling ethanol continues to concentrate, cross 100 orders, with the concentrated solution spray drying, collect spray powder, promptly make the Fructus Crataegi effective ingredient.
2. a kind of preparation method according to claim 1 with Chinese medicinal soft capsule of blood fat reducing and health care, the preparation method that it is characterized in that described Princepia utilis oil active component is: with normal hexane and the described Princepia utilis oil mixed by 5: 2, air-tight state is in-5--10 ℃ refrigerator-freezer in freezing 2-24 hour, satisfied fatty acid is crystalline state and separates out, both are separated, wash satisfied fatty acid repeatedly with normal hexane simultaneously, last concentrating under reduced pressure normal hexane obtains Princepia utilis oil active component unsaturated fatty acid.
3. preparation method with Chinese medicine microcapsule of blood fat reducing and health care, it is characterized in that described microcapsule be by weight the component meter with Princepia utilis oil active component: 10-18; Fructus Crataegi effective ingredient: 1.7-7; Vitamin E: 0.3-3 is a capsule-core, accounts for the 30-90% weight ratio, is softgel shell with gelatin and Radix Acaciae senegalis, and softgel shell accounts for 5-30%, and allocates emulsifying agent, fixative, dispersant, PH regulator into, by existing complex coacervation, makes soft capsule; Each composition weight proportion is: the content of raw material is 30-90%, and gelatin and Radix Acaciae senegalis content are 3-30%, and emulsifier content is 2-40%, curing agent content is 3-45%, dispersant is 0.1-10%, and it is 0.01-0.5% that PH regulates dosage, and each component sum is 100%;
Emulsifying agent is selected any one or more in sucrose ester, span, the tween etc. for use; Firming agent is selected the formalin of 10-37% for use; Dispersant is selected any one or more in alkylsulfonate, the diisopropyl phosphate for use; The PH regulator is selected sodium hydroxide for use, in oxyammonia or the acetic acid any;
The preparation method of described Princepia utilis oil active component is: will clear up and shine the Princepia utilis raw material after coarse powder is removed the peel, is ground into to dry doubling, steaming before the oil expression is fried and is handled after 1-3 hour in the usual way, squeeze and collect the oils and fats of squeezing out with spiral crusher, to its Residual oil≤10%, the solid shape precipitate of filtering promptly obtains operational limpid Princepia utilis oil; During preparation, Princepia utilis is controlled at and makes its water content is that 8-10% and temperature are to steam under 85-115 ℃ the condition to fry to handle; Getting the 15g potassium hydroxide is dissolved in 420ml 95% ethanol, add above-mentioned Princepia utilis oil 60g, in alcohol reflux temperature saponification 45min, stirring makes and reacts completely, and the stirring saponification liquor makes and is cooled to 5 ℃, under this temperature, keep 60min, sucking filtration gets transparent filtrate, concentrates to reclaim ethanol, make ethanol content be not more than 50%, concentrated solution adds the 200ml distilled water, transfers PH to 2 with hydrochloric acid, separates upper strata fluid, 70 ℃ of water-bath preparations contain the alcoholic solution 480ml of certain proportion carbamide, add above gained oil reservoir, 70 ℃ of stirring in water bath make solution be uniform transparent phase, are cooled to 5 ℃, under this temperature, place 6h, the clathrate that removes by filter formation concentrates and reclaims ethanol, adds the 250ml distilled water diluting, uses the 200ml chloroform extraction, reclaim chloroform, obtain spissated Princepia utilis oil active component unsaturated fatty acid;
The preparation method of described Fructus Crataegi effective ingredient is: choose Fructus Crataegi, clear water is cleaned, chopping, the ethanol of the 80-95% that adding 3-10 doubly measures, be heated to 50-80 ℃, reflux, extract, 2-5 time, each extraction time is 2-5 hour, and extracting solution 100 orders are crossed screen cloth, merge ethanol filtrate, decompression recycling ethanol, concentrated solution 100 orders are crossed screen cloth, with macroporous resin column on the filterable concentrated solution, water successively, the 60-80% ethanol elution, collect the 60-80% ethanol elution, decompression recycling ethanol continues to concentrate, cross 100 orders, with the concentrated solution spray drying, collect spray powder, promptly make the Fructus Crataegi effective ingredient.
4. the preparation method with Chinese medicine microcapsule of blood fat reducing and health care according to claim 3, it is characterized in that: the preparation method of described Princepia utilis oil active component is: with normal hexane and the described Princepia utilis oil mixed by 5: 2, air-tight state is in-5-10 ℃ refrigerator-freezer in freezing 2-24 hour, satisfied fatty acid is crystalline state and separates out, both are separated, wash satisfied fatty acid repeatedly with normal hexane simultaneously, last concentrating under reduced pressure normal hexane obtains Princepia utilis oil active component unsaturated fatty acid.
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| CN103933905B (en) * | 2014-05-07 | 2016-02-03 | 河南工业大学 | Oily microballoon of a kind of himalayan prinsepia leaf fruit and preparation method thereof |
| CN104382139B (en) * | 2014-11-13 | 2016-05-18 | 黄琛 | A kind of beverage for acute alcoholism and preparation method thereof |
| CN104643065A (en) * | 2015-01-28 | 2015-05-27 | 郑州大学 | Hawthorn omega-3 soft capsule and preparation method thereof |
| CN105030731B (en) * | 2015-07-07 | 2017-07-11 | 柳华平 | A kind of preparation method of the microcapsules to alcoholic liver injury with protective effect |
| CN107308226B (en) * | 2016-04-27 | 2021-06-01 | 伽蓝（集团）股份有限公司 | Prinsepia utilis royle extract and preparation method and application thereof |
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| CN1739368A (en) * | 2005-07-27 | 2006-03-01 | 山东新良油脂有限公司 | Vitamin-rich nutritive vegetable oil and its prepn process |
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