CN101502307A - Bee pollen sugar-free active instant granule - Google Patents
Bee pollen sugar-free active instant granule Download PDFInfo
- Publication number
- CN101502307A CN101502307A CNA2009100794787A CN200910079478A CN101502307A CN 101502307 A CN101502307 A CN 101502307A CN A2009100794787 A CNA2009100794787 A CN A2009100794787A CN 200910079478 A CN200910079478 A CN 200910079478A CN 101502307 A CN101502307 A CN 101502307A
- Authority
- CN
- China
- Prior art keywords
- preparation
- parts
- bee pollen
- pollen
- wall
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a preparation method and product of instant particles of bee pollen active ingredient and products thereof. The wall of bee pollen is broken using a composite enzymolysis wall-breaking method and the wall-broken bee pollen and the common excipients are processed into the instant particles. The bee pollen is subjected to enzymolysis by the plant composite hydrolase, it is a newest technological process, with marked features such as simpleness, mild condition, easy operation, short time-consumption of reaction process, high wall breaking rate, compared with the other technological process. After the bee pollen is subjected to enzymolysis, the content of the main active ingredient is markedly increased and the sensitizer is subjected to enzymolysis, therefore the nutritive value of the product is greatly increased and the food safety of the bee pollen product is increased and the product quality is increased.
Description
Technical field
The present invention relates to a kind of bee pollen sugar-free active instant granule and preparation method thereof, belong to medicine, field of health care food.
Background technology
Since Compendium of Material Medica, comprehensive composite nutrient of melissa powder and magical effect are subjected to compatriots always and pursue, and have medicinal widely and edible colony at folks of china.In recent years, constantly occur under the background of security incident, pursue balance health, pay attention to food treatment of food therapy and return natural scientific idea returning once again at partial function food and medicine.In recent years, its Products Development utilization research of melissa powder at home and abroad causes widely once more to be carried out, and has caused people's attention.Hay Fever is risen in Europe, the United States, utilizes pollen food, improves the grocery trade nutritive value.China also utilizes pollen to make food additives and Medicines and Health Product, improves nutritive value of food and prevents and cures diseases.
The melissa powder is that honeybee is gathered the pollen load that forms the pollen process from phanerogamous flower, contains the necessary protein of human body (SOD, several amino acids), lipid, carbohydrate, multivitamin (vitamin C, E, A, D, P, B
1, B
6) and trace element (K, P, Fe, Cu, Mn, Mg, Ca, Zn or the like), and human physiological functions had the multiple bioactivators such as flavonoids, nucleic acid, natural phytohormone, sex hormone and promoting sexual gland hormone of special efficacy, it is called as " complete nutrition product " in the world.Have tangible enhancing body immunity, antifatigue, delay senility, improve looks and treat effects such as prostatic disorders.
China is vast in territory, melissa powder aboundresources, particularly in recent years, the plantation area of China's rape increases, thereby bee pollen form cole is the inexpensive melissa powder of high yield, though bee pollen form cole has various benefits to human body, the puckery smell of its intrinsic raw meat and certain sensitization often make the part consumer shy away.
According to the structure of pollen as can be known, ripe pollen wall is divided into tangible 2 layers, i.e. inner and outer wall.Outer wall is thicker, hard and lack flexibility, main component is a cellulose, has characteristic antiacid, that antibiont decomposes.Outer wall has germinal aperature or ditch seam usually, is the weakest place of outer wall, stretches out pollen wall during pollen germination thus.That sporopollenin in its outer wall construction has is acidproof, alkaline-resisting, heatproof, withstand voltage and to hydrochloric acid in gastric juice and the highly stable physicochemical property of other digestive system enzymes, much studies show that when pollen enters the alimentary canal of human body, though can discharge some nutritional labelings by the germinal aperature on surface, but it is few for counting, therefore in order to improve the value of exploiting and utilizing of pollen, it is carried out special broken wall treatment just seem particularly important.
Pollen broken wall is meant that the cell of pollen is subjected to damage in various degree, so that pollen includes release, absorption and the utilization of nutriment.It is a key technology in the pollen processing.
The process for breaking wall of pollen of report comprises Wet-process wall breaking and dry method broken wall both at home and abroad at present.Wet-process wall breaking method mainly contains: difference Poluo wall method, machinery broken wall law, organic solvent broken wall method, fermentation enzymolysis broken wall method etc.; The dry method broken wall mainly contains microwave radiation broken wall method and comminution by gas stream.Existing wall-breaking method respectively has its advantage, but shortcoming is obvious equally, and perhaps long processing period is destroyed bigger to the nutritional labeling of pollen; Perhaps produce a large amount of heats, the forfeiture that causes the pollen enzyme to be lived in the broken wall process; Perhaps complex process and cost costliness increase product cost, are difficult for aborning promoting the use of.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide a kind of preparation method and product of melissa powder active component instant granular, be to adopt the complex enzyme zymohydrolysis wall-breaking method to carry out being prepared into particle with excipients commonly used behind the broken wall of melissa pollen.Plant composite hydrolytic enzyme enzymolysis melissa powder is up-to-date now process, compares with other method, distinguishing features such as this method has simply, mild condition, operation easily, course of reaction weak point consuming time, sporoderm-broken rate height.The melissa powder is behind enzymolysis, and main active content wherein significantly improves, and contained sensitizer is decomposed by enzymolysis, thereby has both increased substantially the nutritive value of product, has improved the edible safety of pollen product again, promotes product quality.
In order to reach described purpose, the invention provides a kind of preparation method of melissa powder active component instant granular, it mainly may further comprise the steps:
1. preparation citric acid solution: citric acid 5.50-50.50g and natrium citricum 14.70-60.00g are dissolved in the 150-200ml deionized water, and the pH value of adjusting cushioning liquid is 2.5-6.8, gets 60-120ml cushioning liquid and pours in the conical flask of 250ml;
2. with described cushioning liquid 0.05-0.50Mpa in autoclave, sterilization 15-45min, it is standby to take out the cooling back;
3. get plant composite hydrolytic enzyme 80-400 μ l, it is joined in the cushioning liquid of above-mentioned cooling to dissolving, the melissa powder 5-45g that takes by weighing again through hot air sterilization joins in this conical flask, and is evenly mixed;
4. shaking table is cultivated: at rotating speed 120-250rpm, and reaction temperature 30-45 ℃, extraction time 2-10h, the condition of pH2.5-6.8 guarantees that pollen enzymolysis broken wall is abundant;
5. reaction mixture gets extract through filtration under diminished pressure, measures the extract cumulative volume, and surveys its general flavone, SOD enzyme activity, gets the 50ml freeze drying again and obtains concentrate, calculates melissa powder recovery rate.
6. the insoluble solid after will filtering (its main component comprises cellulose, hemicellulose, pollen polysaccharide, fat, protein and mineral matter) sampling is 40-120 ℃ of thermostatic drying chamber oven dry, to water content 2.5-12.5%.
7. granulate: will make particle after above-mentioned concentrate, insoluble solid and materia medica excipients (Sugarless type) commonly used, the solvent.
Wherein, described plant composite hydrolytic enzyme is selected from more than one arbitrary proportion mixtures in cellulase, pectase, the protease, preferred cellulase: pectase: the weight ratio of protease is 1-5:1-5:1-5, more preferably cellulase: pectase: the weight ratio of protease is 1:1:1.
Described shaking table culture condition is preferably 45 ℃ of reaction temperatures, extraction time 6h, pH3.4.
Granulation in the described step 7 for example can be adopted described prescription of pharmacopeia and method.In preferred embodiment of the present invention, adopt following method:
Take by weighing the raw material of following parts by weight respectively: described concentrate 20-80 part, soluble starch 20-50 part, insoluble solid 10-90 part, xylitol 2-10 part, carboxymethyl cellulose 2-10 part and maltodextrin 10-50 part mix, adding ethanolic solution 30-100ml lives into bulk in right amount with flavoring essence, on 20 mesh sieves, be twisted into particle, scatter, dried 1-4 hour for 30-60 ℃.Ventilate and cool off, pack.
Wherein, preferably, be that 50 parts of described concentrates, 30 parts of soluble starches, 20 parts of insoluble solids, 3 parts of xylitols, 4 parts of carboxymethyl celluloses and maltodextrin are mixed for 25 parts, add the 50ml ethanolic solution and become bulk with the flavoring essence work that accounts for raw material gross weight 0.5-1%, on 20 mesh sieves, be twisted into particle, scatter, dried 2 hours for 40 ℃.
Wherein, described ethanolic solution be distilled water and absolute ethyl alcohol 1:4-4:1 is formulated by volume, preferred volume ratio 1:1.
The extract cumulative volume for preparing through the inventive method is 200-280ml, and melissa powder recovery rate is 25-55%, and the insoluble solid water content is 2.5-12.5%.Prepared cole melissa powder particles carries out the physical and chemical quality inspection by 2005 editions granules of Chinese Pharmacopoeia.
The enzymolysis melissa powder particles sporoderm-broken rate height that the present invention obtains, active component content improves greatly, and the pollen flavones content has on average improved 2.07 times, and pollen SOD enzyme activity also has higher reservation.Its organoleptic indicator of enzymolysis pollen also has remarkable lifting: the pollen particles nature aberration that color is caused by enzymolysis cause flower source difference, and it is soft faint yellow to be improved as homogeneous; Mouthfeel by enzymolysis before little astringent taste, be improved as and have the pure and fresh naturally and low sweet taste of plant.Pollen is behind enzymolysis, and anaphylactogen is hydrolyzed destruction, has improved product bio-safety degree.
Description of drawings
Fig. 1 is a melissa powder granulated quickly dissolving exploded view;
Fig. 2 is melissa powder granulated quickly dissolving 5min dissolving figure;
Fig. 3 is a melissa powder granulated quickly dissolving exploded view.
The specific embodiment
The invention will be further described below in conjunction with embodiment, it should be understood that these embodiment only are used for the purpose of illustration, never limit protection scope of the present invention.
[reference examples 1] this example is the blank group.
The preparation citric acid solution is dissolved in citric acid 5.50-50.50g and natrium citricum 14.70-60.00g in the 200ml deionized water, and the pH value of adjusting cushioning liquid is got 80ml cushioning liquid and poured in the conical flask of 250ml to 2.5-6.8; With described cushioning liquid 0.1Mpa in autoclave, sterilization 20min, taking-up cooling back is standby;
20g is joined in the above-mentioned buffer solution through the melissa powder of hot air sterilization, and last shaking table is cultivated, and is 120rpm at rotating speed, 45 ℃ of reaction temperatures, and extraction time 6h, under the condition of pH3.4, abundant broken wall.Reaction mixture is behind decompress filter, and the extracting liquid volume that measures is 200ml, gets 50ml afterwards and concentrates, and the liquid cooling freeze-drying is dry, and recording its general flavone content is 247.249mg/100g, and its SOD enzyme activity is 203.160U/mL.
With the insoluble solid behind suction filtration sampling, dry at 40-120 ℃ of constant temperature oven that to calculate water content to constant weight be 4.5%.
Above-mentioned concentrate 50g, soluble starch 30g, insoluble solid 20g, xylitol 3g, carboxymethyl cellulose 4g and maltodextrin 25g are mixed, adding 50ml ethanolic solution (1:1 configuration by volume forms distilled water with absolute ethyl alcohol) and flavoring essence (account for raw material gross weight 0.6%) lives into bulk, on 20 mesh sieves, be twisted into particle, scatter, 40 ℃ of oven dry promptly got particle of the present invention in 2 hours.
[embodiment 1]
The preparation of cushioning liquid is with reference examples 1.Getting 80 μ l plant composite hydrolytic enzymes (cellulase: pectase: the weight ratio of protease is 1:1:1) and 20g melissa powder and join simultaneously in the buffer solution, is 120rpm at rotating speed, 45 ℃ of reaction temperatures, and extraction time 6h, under the condition of pH3.4, abundant broken wall.Reaction mixture is behind decompress filter, and the extracting liquid volume that measures is 250ml, and get 50ml afterwards and concentrate, freeze drying, recording its general flavone content is 335.520mg/100g, its SOD enzyme activity is 149.96U/mL.
With the insoluble solid behind suction filtration sampling, dry at 40-120 ℃ of constant temperature oven that to calculate water content to constant weight be 5.0%.
Above-mentioned concentrate 50g, soluble starch 30g, insoluble solid 20g, xylitol 3g, carboxymethyl cellulose 4g and maltodextrin 25g are mixed, adding 50ml ethanolic solution (1:1 configuration by volume forms distilled water with absolute ethyl alcohol) and flavoring essence (account for raw material gross weight 0.6%) lives into bulk, on 20 mesh sieves, be twisted into particle, scatter, 40 ℃ of oven dry promptly get particle of the present invention.
[embodiment 2]
The preparation of cushioning liquid is with reference examples 1.Getting 80 μ l plant composite hydrolytic enzymes (cellulase: pectase: the weight ratio of protease is 2:1:1) and 20g melissa powder and join simultaneously in the buffer solution, is 150rpm at rotating speed, 45 ℃ of reaction temperatures, and extraction time 6h, under the condition of pH3.4, abundant broken wall.Reaction mixture is behind decompress filter, and the extracting liquid volume that measures is 280ml, and get 50m afterwards and concentrate, freeze drying, recording its general flavone content is 352.603mg/100g, its SOD enzyme activity is 203.15U/mL.
With the insoluble solid behind suction filtration sampling, dry at 60 ℃ of constant temperature ovens that to calculate water content to constant weight be 2.5%.
Above-mentioned concentrate 20g, soluble starch 50g, insoluble solid 50g, xylitol 7g, carboxymethyl cellulose 6g and maltodextrin 25g are mixed, adding 70ml ethanolic solution (1:4 configuration by volume forms distilled water with absolute ethyl alcohol) and flavoring essence (account for raw material gross weight 1%) lives into bulk, on 20 mesh sieves, be twisted into particle, scatter, 40 ℃ of oven dry promptly get particle of the present invention.
[embodiment 3]
The preparation of cushioning liquid is with reference examples 1.Getting 100 μ l plant composite hydrolytic enzymes (cellulase: pectase: the weight ratio of protease is 5:3:1) and 40g melissa powder and join simultaneously in the buffer solution, is 150rpm at rotating speed, 45 ℃ of reaction temperatures, and extraction time 10h, under the condition of pH6.8, abundant broken wall.Reaction mixture is behind decompress filter, and the extracting liquid volume that measures is 250ml, gets the freeze drying of 50ml extract afterwards and obtains concentrate, and recording its general flavone content is 389.063mg/100g, and its SOD enzyme activity is 214.14U/mL.
With the insoluble solid behind suction filtration sampling, dry at 100 ℃ of constant temperature ovens that to calculate water content to constant weight be 10.0%.
Above-mentioned concentrate 80g, soluble starch 40g, insoluble solid 10g, xylitol 8g, carboxymethyl cellulose 4g and maltodextrin 15g are mixed, adding 80ml ethanolic solution (4:1 configuration by volume forms distilled water with absolute ethyl alcohol) and flavoring essence (account for raw material gross weight 0.7%) lives into bulk, on 20 mesh sieves, be twisted into particle, scatter, 60 ℃ of oven dry promptly got particle of the present invention in 2 hours.
[embodiment 4]
The preparation of cushioning liquid is with reference examples 1.Getting 200 μ l plant composite hydrolytic enzymes (cellulase: pectase: the weight ratio of protease is 1:4:2) and 30g melissa powder and join simultaneously in the buffer solution, is 150rpm at rotating speed, 40 ℃ of reaction temperatures, and extraction time 4h, under the condition of pH3.4, abundant broken wall.Reaction mixture is behind decompress filter, and the extracting liquid volume that measures is 210ml, gets the freeze drying of 50ml extract afterwards and obtains concentrate, and recording its general flavone content is 439.063mg/100g, and its SOD enzyme activity is 256.61U/mL.
With the insoluble solid behind suction filtration sampling, dry at 80 ℃ of constant temperature ovens that to calculate water content to constant weight be 6.0%.
Above-mentioned concentrate 20g, soluble starch 20g, insoluble solid 90g, xylitol 8g, carboxymethyl cellulose 10g and maltodextrin 40g are mixed, adding 100ml ethanolic solution (1:1 configuration by volume forms distilled water with absolute ethyl alcohol) and flavoring essence (account for raw material gross weight 0.8%) lives into bulk, on 20 mesh sieves, be twisted into particle, scatter, 40 ℃ of oven dry promptly got particle of the present invention in 4 hours.
[embodiment 5]
The preparation of cushioning liquid is with reference examples 1.Getting 300 μ l plant composite hydrolytic enzymes (cellulase: pectase: the weight ratio of protease is 2:4:3) and 10g melissa powder and join simultaneously in the buffer solution, is 150rpm at rotating speed, 35 ℃ of reaction temperatures, and extraction time 6h, under the condition of pH4.5, abundant broken wall.Reaction mixture is behind decompress filter, and the extracting liquid volume that measures is 270ml, gets the freeze drying of 50ml extract afterwards and obtains concentrate, and recording its general flavone content is 472.190mg/100g, and its SOD enzyme activity is 267.30U/mL.
With the insoluble solid behind suction filtration sampling, dry at 120 ℃ of constant temperature ovens that to calculate water content to constant weight be 5.8%.
Above-mentioned concentrate 50g, soluble starch 30g, insoluble solid 60g, xylitol 2g, carboxymethyl cellulose 10g and maltodextrin 15g are mixed, adding 40ml ethanolic solution (2:1 configuration by volume forms distilled water with absolute ethyl alcohol) and flavoring essence (account for raw material gross weight 0.9%) lives into bulk, on 20 mesh sieves, be twisted into particle, scatter, 20 ℃ of oven dry promptly got particle of the present invention in 3 hours.
[embodiment 6]
The preparation of cushioning liquid is with reference examples 1.Getting 400 μ l plant composite hydrolytic enzymes (cellulase: pectase: the weight ratio of protease is 5:2:4) and 20g melissa powder and join simultaneously in the buffer solution, is 120rpm at rotating speed, 45 ℃ of reaction temperatures, and extraction time 5h, under the condition of pH3.4, abundant broken wall.Reaction mixture is behind decompress filter, and the extracting liquid volume that measures is 240ml, gets the freeze drying of 50ml extract afterwards and obtains concentrate, and recording its general flavone content is 512.813mg/100g, and its SOD enzyme activity is 320.77U/mL.
With the insoluble solid behind suction filtration sampling, dry at 105 ℃ of constant temperature ovens that to calculate water content to constant weight be 11.2%.
Above-mentioned concentrate 50g, soluble starch 40g, insoluble solid 10g, xylitol 3g, carboxymethyl cellulose 10g and maltodextrin 25g are mixed, adding 50ml ethanolic solution (1:3 configuration by volume forms distilled water with absolute ethyl alcohol) and flavoring essence (account for raw material gross weight 0.5%) lives into bulk, on 20 mesh sieves, be twisted into particle, scatter, 40 ℃ of oven dry promptly got particle of the present invention in 1 hour.
The above only is preferred embodiment of the present invention, only is illustrative for the purpose of the present invention, and nonrestrictive.Those skilled in the art is understood, and can carry out many changes to it in the spirit and scope that claim of the present invention limited, revise, even equivalence, but all will fall within the scope of protection of the present invention.
Claims (10)
1, a kind of preparation method of melissa powder active component instant granular is characterized in that, mainly may further comprise the steps:
A. prepare citric acid solution: citric acid 5.50-50.50g and natrium citricum 14.70-60.00 are dissolved in the 150-200ml deionized water, and the pH value of adjusting cushioning liquid is got 60-120ml cushioning liquid and is poured in the conical flask of 250ml to 2.5-6.8;
B. with described cushioning liquid 0.05-0.50Mpa in autoclave, sterilization 15-45min, it is standby to take out the cooling back;
C. get plant composite hydrolytic enzyme 80-400 μ l, it is joined in the cushioning liquid of above-mentioned cooling to dissolving, the melissa powder 5-45g that takes by weighing again through hot air sterilization joins in this conical flask, and is evenly mixed;
D. shaking table is cultivated: at rotating speed 120-250rpm, reaction temperature 30-45 ℃, extraction time 2-10h under the condition of pH2.5-6.8, guarantees that pollen enzymolysis broken wall is abundant;
E. reaction mixture gets extract through filtration under diminished pressure, and freeze drying obtains concentrate;
F. the insoluble solid after will filtering is dried to constant weight at 40-120 ℃ of constant temperature oven;
G. granulate: will make particle after described concentrate, described insoluble solid and materia medica Sugarless type excipients commonly used, the solvent.
2, preparation method according to claim 1 is characterized in that described plant composite hydrolytic enzyme is selected from two kinds and two or more arbitrary proportion mixture in cellulase, pectase, the protease.
3, preparation method according to claim 2 is characterized in that described plant composite hydrolytic enzyme is cellulase 1-5 part, pectase 1-5 part, and protease 1-5 part is weight portion.
4, preparation method according to claim 3 is characterized in that the weight ratio that described plant composite hydrolytic enzyme is cellulase, pectase, protease is the mixture of 1:1:1.
5, preparation method according to claim 1 is characterized in that described shaking table culture condition is preferably 45 ℃ of reaction temperatures, extraction time 6h, pH3.4.
6, preparation method according to claim 1, it is characterized in that described granulation may further comprise the steps, take by weighing the raw material of following parts by weight respectively: described concentrate 20-80 part, soluble starch 20-50 part, insoluble solid 10-90 part, xylitol 2-10 part, carboxymethyl cellulose 2-10 part and maltodextrin 10-50 part mix, adding the 30-100ml ethanolic solution lives into bulk in right amount with flavoring essence, on 20 mesh sieves, be twisted into particle, scatter, dried 1-4 hour for 30-60 ℃.
7, preparation method according to claim 6, it is characterized in that described granulation may further comprise the steps, take by weighing the raw material of following parts by weight respectively: 50 parts of described concentrates, 30 parts of soluble starches, 20 parts of insoluble solids, 3 parts of xylitols, 4 parts of carboxymethyl celluloses and maltodextrin mix for 25 parts, add the 50ml ethanolic solution and become bulk with the flavoring essence work that accounts for raw material gross weight 0.5-1%, on 20 mesh sieves, be twisted into particle, scatter, dried 2 hours for 40 ℃.
8,, it is characterized in that described ethanolic solution is that 1:4-4:1 is formulated by volume for distilled water and absolute ethyl alcohol according to claim 6 or 7 described preparation methods.
9, a kind of melissa powder active component instant granular is characterized in that it prepares according to the described method of claim 1-8.
10, the described melissa powder of claim 9 active component instant granular is as the purposes of food additives or Medicines and Health Product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100794787A CN101502307B (en) | 2009-03-12 | 2009-03-12 | Bee pollen sugar-free active instant granule |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100794787A CN101502307B (en) | 2009-03-12 | 2009-03-12 | Bee pollen sugar-free active instant granule |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101502307A true CN101502307A (en) | 2009-08-12 |
| CN101502307B CN101502307B (en) | 2012-05-23 |
Family
ID=40974802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100794787A Expired - Fee Related CN101502307B (en) | 2009-03-12 | 2009-03-12 | Bee pollen sugar-free active instant granule |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101502307B (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999568A (en) * | 2010-09-21 | 2011-04-06 | 湖北国力生物技术开发有限公司 | Biological wall breaking technology of pine pollen |
| CN102948656A (en) * | 2012-10-26 | 2013-03-06 | 北京知蜂堂蜂产品有限公司 | Method for wall breaking, desensitization and water-soluble component extraction of pollen and water-soluble pollen cream prepared by the method |
| CN103039709A (en) * | 2013-01-11 | 2013-04-17 | 江西旺大动物科技有限公司 | Preparation method of wall-breaking and enzymolysis bee pollen |
| CN103535835A (en) * | 2012-07-13 | 2014-01-29 | 北京市蜂业公司 | Solid beverage prepared from pollen soluble extracts |
| CN104957456A (en) * | 2015-06-09 | 2015-10-07 | 西北大学 | Fagopyrum esculentum pollen composite instant particle and processing method thereof |
| CN105533595A (en) * | 2015-12-09 | 2016-05-04 | 杨佳林 | Bee pollen gel food and processing method thereof |
| CN105639653A (en) * | 2015-12-23 | 2016-06-08 | 华南农业大学 | oil-tea camellia flower granule with antioxidant activity and preparation method and application thereof |
| CN106490556A (en) * | 2016-10-14 | 2017-03-15 | 浙江金童生物科技有限公司 | A kind of sugar-free or the manufacture method of low sugar bee pollen |
| CN108719904A (en) * | 2018-04-11 | 2018-11-02 | 湖南农业大学 | A kind of Bee Pollen and its processing method |
| CN109170902A (en) * | 2018-11-07 | 2019-01-11 | 中国科学院兰州化学物理研究所 | A kind of fructus lycii Bee Pollen Amti-sanility health-care food and preparation method thereof |
| CN109170733A (en) * | 2018-08-23 | 2019-01-11 | 杭州康力食品有限公司 | A kind of preparation method of Bee Pollen |
| CN110050977A (en) * | 2019-05-28 | 2019-07-26 | 西北农林科技大学 | A kind of peony pollen electuary and preparation method thereof |
| CN110613113A (en) * | 2019-09-24 | 2019-12-27 | 武汉市农业科学院 | Preparation method of functional biological fermentation bee pollen |
| CN110810783A (en) * | 2019-11-21 | 2020-02-21 | 青海民族大学 | Method for breaking cell wall of rape bee pollen |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321442A (en) * | 2000-04-30 | 2001-11-14 | 王振宇 | Pollen concentrated beverage and its production method |
-
2009
- 2009-03-12 CN CN2009100794787A patent/CN101502307B/en not_active Expired - Fee Related
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999568A (en) * | 2010-09-21 | 2011-04-06 | 湖北国力生物技术开发有限公司 | Biological wall breaking technology of pine pollen |
| CN103535835A (en) * | 2012-07-13 | 2014-01-29 | 北京市蜂业公司 | Solid beverage prepared from pollen soluble extracts |
| CN103535835B (en) * | 2012-07-13 | 2015-09-30 | 北京市蜂业公司 | Solid beverage prepared by a kind of pollen soluble extract |
| CN102948656A (en) * | 2012-10-26 | 2013-03-06 | 北京知蜂堂蜂产品有限公司 | Method for wall breaking, desensitization and water-soluble component extraction of pollen and water-soluble pollen cream prepared by the method |
| CN102948656B (en) * | 2012-10-26 | 2014-04-09 | 北京知蜂堂蜂产品有限公司 | Method for wall breaking, desensitization and water-soluble component extraction of pollen and water-soluble pollen cream prepared by method |
| CN103039709A (en) * | 2013-01-11 | 2013-04-17 | 江西旺大动物科技有限公司 | Preparation method of wall-breaking and enzymolysis bee pollen |
| CN104957456A (en) * | 2015-06-09 | 2015-10-07 | 西北大学 | Fagopyrum esculentum pollen composite instant particle and processing method thereof |
| CN105533595B (en) * | 2015-12-09 | 2019-08-02 | 杨佳林 | A kind of Bee Pollen gel food and its processing method |
| CN105533595A (en) * | 2015-12-09 | 2016-05-04 | 杨佳林 | Bee pollen gel food and processing method thereof |
| CN105639653A (en) * | 2015-12-23 | 2016-06-08 | 华南农业大学 | oil-tea camellia flower granule with antioxidant activity and preparation method and application thereof |
| CN106490556A (en) * | 2016-10-14 | 2017-03-15 | 浙江金童生物科技有限公司 | A kind of sugar-free or the manufacture method of low sugar bee pollen |
| CN108719904A (en) * | 2018-04-11 | 2018-11-02 | 湖南农业大学 | A kind of Bee Pollen and its processing method |
| CN108719904B (en) * | 2018-04-11 | 2021-10-26 | 湖南农业大学 | Bee pollen and processing method thereof |
| CN109170733A (en) * | 2018-08-23 | 2019-01-11 | 杭州康力食品有限公司 | A kind of preparation method of Bee Pollen |
| CN109170902A (en) * | 2018-11-07 | 2019-01-11 | 中国科学院兰州化学物理研究所 | A kind of fructus lycii Bee Pollen Amti-sanility health-care food and preparation method thereof |
| CN110050977A (en) * | 2019-05-28 | 2019-07-26 | 西北农林科技大学 | A kind of peony pollen electuary and preparation method thereof |
| CN110613113A (en) * | 2019-09-24 | 2019-12-27 | 武汉市农业科学院 | Preparation method of functional biological fermentation bee pollen |
| CN110810783A (en) * | 2019-11-21 | 2020-02-21 | 青海民族大学 | Method for breaking cell wall of rape bee pollen |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101502307B (en) | 2012-05-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101502307B (en) | Bee pollen sugar-free active instant granule | |
| CN104872758B (en) | The preparation method of cicada fungus burdock oral liquid | |
| CN106343090A (en) | Fructus siraitiae, prebiotic and chrysanthemum flower tea instant beverage and preparation method thereof | |
| CN101829187B (en) | Preparation method of fruit-flavored cyclocarya paliurus chewable tablets | |
| CN101791119A (en) | Chaga nutrient composition | |
| CN106389541A (en) | Cistanche deserticola extract and preparation method and application thereof | |
| WO2019007237A1 (en) | Lycium ruthenicum effervescent tablet and preparation method therefor | |
| CN105012707A (en) | Dendrobium officinale capsules and preparation method thereof | |
| CN111728217A (en) | Maca and natto product composite preparation and preparation method thereof | |
| CN101129790A (en) | Compound hippophae rhamnoides lozenge method of producing the same | |
| CN103372047A (en) | Eucommia bark buccal tablets and preparation method thereof | |
| WO2019174369A1 (en) | Method for using tea seed meal as additive for aquatic animals and ruminants | |
| CN101972029B (en) | Tianshan yacon granule and preparation method thereof | |
| CN105012778A (en) | Dendrobium officinale-coix seed capsules and preparation method thereof | |
| CN105580953A (en) | Mulberry tabletting candy production method | |
| CN107411068B (en) | Red date cyclic adenosine monophosphate chewable tablet and preparation method thereof | |
| CN105851764A (en) | Cyclocarya paliurus leaf solid beverage, as well as preparation method and application thereof | |
| CN110338426A (en) | A kind of blood enriching effervescent tablet agent and preparation method thereof | |
| WO2019144779A1 (en) | Preparation method and use method of tea dreg being directly used as additive that promotes healthy growth of animal | |
| CN104474007A (en) | Antipyretic artemisia apiacea and licorice particle and soluble pulvis for veterinary use and preparation method thereof | |
| CN107080264A (en) | A kind of special pine kernel protein composite chewable tablet of three high crowd | |
| JP2005060259A (en) | Stomach ache-inhibiting or preventing agent and food or drink composition | |
| JP2005060259A6 (en) | An agent for suppressing or preventing gastric pain and a composition for food and drink. | |
| CN105876036A (en) | Blood sugar reducing effervescent tablet herbal tea and preparation method thereof | |
| CN106343104A (en) | Making method of care powder with fructus trichosanthis and dendrobe |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120523 Termination date: 20140312 |