CN101496497A - Seedling exercising technique for test-tube plantlet with full sunshine and spray - Google Patents
Seedling exercising technique for test-tube plantlet with full sunshine and spray Download PDFInfo
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- CN101496497A CN101496497A CNA2008100320790A CN200810032079A CN101496497A CN 101496497 A CN101496497 A CN 101496497A CN A2008100320790 A CNA2008100320790 A CN A2008100320790A CN 200810032079 A CN200810032079 A CN 200810032079A CN 101496497 A CN101496497 A CN 101496497A
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Abstract
The invention discloses full light spray seedling hardening technology for test-tube seedling. The invention mainly adopts a JH-3 computer control instrument adjusting and controlling spray system device which hardens the test-tube seedlings at proper temperature and humidity. The technology has the advantages of high seedling hardening survival rate, short cycle, low cost, good seedling quality, and the like. The survival rate can be improved from originally 30 to 40 percent to above 96 percent; and the establishment percentage can be improved to 98 percent. Therefore, the technology overcomes the problems of low survival rate and poor seedling quality caused by the factors such as strong light and moisture diminishing during the transplantation and hardening of the test-tube seedlings, and opens up a new way for the plant tissue culture induced rootage and seedling hardening.
Description
Technical field
The present invention relates to a kind of cultivating seedlings technology, especially seedling exercising technique for test-tube plantlet with full sunshine and spray, belong to agricultural production.
Background technology
Before the present invention was unexposed, the test tube full sunshine and spray hardening technology of growing seedlings did not also appear.Plant Tissue Breeding is a kind of high-tech seedling growing process of current agricultural and the future of agriculture development.Plant Tissue Breeding is exactly plant organ pipes such as the root that extracts plant, stem, leaf, bud under conditions such as aseptic and suitable synthetic medium and illumination, temperature, can induce callus, indefinite bud, adventive root, forms complete plantlet at last.It has influence few with material, that speed soon, is not subjected to natural conditions such as weather, season, and plant can remove virus fully, saves advantages such as a large amount of man power and materials.Hardening is that test-tube plantlet is transplanted to one of important step of outdoor cultivation in the Plant Tissue Breeding from indoor test tube, be that the test-tube plantlet hardening is to enter into natural environment from the test tube of the stable environment of aseptic, temperature, humidity, illumination, carry out the transition to the autotrophy process from heterotrophism.The method of past hardening, at first opening the test tube bottle stopper places sunny place and allows it take exercise 1～2 day, take out seedling then and medium is rinsed out, be transplanted in the matrix of compositions such as peat, perlite, vermiculite, rice chaff ash, will shade after the transplanting with warm water, cover with plastic film, keep high humidity of air, sunlight is directly shone, will ventilate and moisturizing in 7～15 days, after treating that 20～45 days young sprouts begin growth, seedling can change normal management over to.The method of this hardening trouble and recruitment is many, careless slightly will mortality, even it is also lower to manage well survival rate, have only about 35%.Therefore, improve test-tube plantlet hardening survival rate, capture test-tube plantlet, become agro-technician and contemporary peasant's an urgent demand from an indoor difficult problem that moves to a large amount of dead seedlings the outdoor hardening process of growing seedlings.
Summary of the invention
The object of the present invention is to provide a kind of test-tube plantlet full exposure spraying hardening technology in the plant tissue culture seedlings-raising, it reduces the hardening cost, reduce labor intensity, use manpower and material resources sparingly, suitable breeding is wide in variety, the survival rate height, the hardening survival rate can be brought up to more than 96% by original 30-40%, and planting percent can bring up to 98%.
For achieving the above object, the present invention is realized by following technical proposals.A kind of test-tube plantlet full exposure spraying hardening technology is that test-tube plantlet is from the indoor important step that moves to outdoor cultivation in the plant tissue culture seedlings-raising, and its technical method is:
(1) the seedbed preparing nursery bed is selected the good level land of ventilated illumination condition, and the seedbed is made as circle, 10-14 meters of diameters; Can be divided into 1-1.5 meters wide nursery in the seedbed, leave 0.2-0.3 meter space between the nursery is adjacent; The nursery bottom is a cobble, and medium sand that upper berth one deck is 30-40 centimetres or perlite etc. are made medium.
(2) spraying installation spraying system device capital equipment is spraying water pipe, JH-3 computer controller, magnetic valve etc.Spraying rotation both arms and center mechanical device etc. are installed at the center, seedbed, after JH-3 computer controller is set corresponding data, the different temperatures in its visual seedbed, humidity are regulated spraying to the test tube seedling automatically, by the temperature and humidity of spraying and regulating the seedbed, the test tube seedling is grown under suitable condition in the hardening process, can guarantee the hardening safety and the success of seedling.
(3) to carry out once loose and routine disinfection to the matrix in seedbed before transplanting and management is transplanted; Will add IBA with 1,2, No. 5 growth-promoting media respectively to its different seeds during test-tube seedling transplanting handles the test-tube plantlet base portion.
(4) hardening management test-tube seedling transplanting is carried out full exposure spraying hardening behind the seedbed, regulates spray time flow etc. with JH-3 controller, and its optimal parameter is set to: 1. seedling is preceding 8 days, it is 50 that basic parameter is set, water spraying time is 70, and evaporation coefficient is 50-70, and water stopping time is 10; 2. seedling is 8-20 days, and it is 60 that basic parameter is set, and water spraying time is 45-55, and evaporation coefficient is 80-100, and water stopping time is 20; 3. seedling is 20-35 days, and it is 70 that basic parameter is set, and water spraying time is 45-55, and evaporation coefficient is 120-150, and water stopping time is 45.In addition, add IBA etc. every one time 0.5% growth-promoting media of spray in 7-10 days during the hardening;
(5) the management test-tube plantlet during little spray is after the hardening of a wheat harvesting period, and the seedling growing way is vigorous day by day, move to little spray nursery thin planting and grow seedlings, and the seedbed requires smooth, and the soil is fertile, adopts JH-3 controller that management is set equally; The parameter setting of controller: 1. seedling early stage, it is 120 that basic parameter is set, and water spraying time is 100, and evaporation coefficient is 150-200, and water stopping time is 60; 2. in the seedling later stage, it is 140 that basic parameter is set, and water spraying time is 60, and evaporation coefficient is 200, and water stopping time is 120; During this, every 10---one time 0.6% growth-promoting media of spray in 15 days adds 0.4% potassium dihydrogen phosphate etc.; Seedling is tending towards prosperous and powerful after common 40 days, can close the computer controller, thereby removes the automated intelligent management, and growing seedlings enters common daily management.In whole hardening process, woody parameter setting with draft and different seeds test-tube plantlets is distinguished to some extent usually, needs corresponding fine setting.
Plant Tissue Breeding is a modern biotechnology, its can be numerous soon various name, special, new, excellent plant seedling, it is fast not only to have propagation, stabilization characteristics of genetics, growth coefficient height, the characteristics of quality better, have more with short production cycle, can produce throughout the year, not be subjected to weather, environmental influence, incomparable advantage be arranged with conventional seedbed system.Hardening be in the tissue culture test-tube plantlet from an indoor important step of being transplanted to outdoor cultivation, traditional hardening mode is to transplant the back by shading, cover with plastic film, keep high humidity of air, sunlight is directly shone, often will be with manually ventilating and moisturizing, thereby, can't reach best regulating and controlling temperature and humidity, so seedling death phenomenon is very serious during hardening.Test-tube plantlet full exposure spraying hardening technology of the present invention, system and the mechanical system of full light auto spraying because test-tube plantlet computerizeds control in the hardening process, insert water source energy auto-control temperature and humidity under the full exposure condition, all the time can make the blade face and the root system of seedling keep normal water content and required temperature and humidity, keep the normal expansion of cell to press, even in test tube, also there is not the still incomplete seedling of root of hair or root of hair like this, be to plant the seedling that abandons alive in the past, also can be smelt strong sprout.Again because at full optical condition lower refining seedling, high temperature and high light, seedling not only can be not withered owing to obtained sufficient photosynthesis, and it is rapid more to take root on the contrary, and it is vigorous more to grow.Advantages such as therefore, the present invention has the survival rate of seedling height, the cycle is short, cost is low, seedling quality is good.Survival rate can be brought up to more than 96% by original 30-40%, if add the seedling that abandons that the incomplete Buddhist monk of taking root do not take root, survival rate also can be turned over again so.Planting percent can improve more than 98%.Thereby captured little transplantation of seedlings and hardening and caused the low and ropy difficult problem of seedling of survival rate, for Plant Tissue Breeding root induction and hardening have been opened up a new way because of factors such as high light and dehydrations.
Embodiment:
Below grow seedlings by strawberry and further to set forth the present invention, the beneficial effect of test-tube plantlet full sunshine and spray hardening.
The strawberry seedling exercising technique for test-tube plantlet with full sunshine and spray
The strawberry berry is scarlet gorgeous, and sweet acid is good to eat, and is nutritious, is regarded as treasure in the fruit.Strawberry is planted seedling then and just can benefit then; Special adopt tissue cultivating seedling, owing to removed virus fully, so the well developed root system growth is vigorous, the output height, and with the tradition ratio of growing seedlings, but output quadrupling the gross annual value of China's industrial and agricultural production in the two decades is a kind of small investment, economic crops that income is high.Per mu yield can reach 2500 kilograms, and the mu income is more than 15000 yuan.Strawberry also can be processed into jam, fruit juice, fruit wine, beverage, fruitcake, preserved fruit and numerous food except that eating raw.Be convenient to the storage transportation, prolong market supply and add the duration.In recent years, strawberry jam and quick-frozen strawberry that China produces export in a large number, for country has exchanged a large amount of foreign exchanges for.Therefore, the development strawberry produces not only to people provide abundant nutrition, satisfies people's life requirement, and significant to improving farmers' income, foreign exchange earning, development local economy.The present invention grows seedlings to strawberry, uses agriculture modern high technology Plant Tissue Breeding, especially adopts strawberry test-tube plantlet full exposure spraying hardening technology.Facts have proved, can effectively reduce production costs, reduce labor intensity, plant percent height, advantage such as quality is good.Survival rate is brought up to more than 96% by original 35%, and planting percent can be brought up to more than 98%.
Strawberry tissue culture in early stage and plant tissue culture media are originally identical, promptly cultivate the strawberry test-tube plantlet in advance: the stem apex that extracts strawberry, with soap lye etc. it is cleaned up, use flushing with clean water again, blot surface moisture with blotting paper then, soak the several seconds taking-up with 70% alcohol and afterwards in time soaked 10～20 minutes with 10% calcium hypochlorite and supernatant.Aseptic water washing 3 times of sterilization back are blotted inoculation with sterile gauze or aseptic paper.Be seeded on the superclean bench and carry out, strawberry is seeded on the medium such as MS+BA that disinfected carries out initial culture, midway through successive transfer culture repeatedly, be inoculated on the root media then, grown root system through the most of strawberry test-tube plantlet of more than 10 days culture of rootage, test-tube plantlet that all take root, that do not take root fully as yet and that have only callus not take root is cleaned up with warm water, be stained with No. 3 growth-promoting medias and soak root, all be transplanted on the full exposure auto spraying seedbed and carry out hardening, all grow new root after common more than 10 days.The strawberry seedling exercising technique for test-tube plantlet with full sunshine and spray is as follows:
(1) seedbed prepare in the strawberry tissue cultivating and seedling test-tube plantlet from indoor move on to outdoor before, to carry out seedbed preparation earlier.The good level land of ventilated illumination condition is selected in the seedbed, and for ease of spraying, the seedbed is set to circle usually, 10-14 meters of diameters; Can be divided into 1-1.5 meters wide nursery in the seedbed, also can be divided into fan-shapedly, leave 0.2-0.3 meter space between the nursery is adjacent; So as the walking and seedling stage conventional maintenance, the nursery bottom is a cobble, medium sand or perlite that upper berth one deck is 30-40 centimetres are made medium.
(2) spraying apparatus installation spraying system device capital equipment is (there is confession in this device market) such as spraying water pipe, computer controller, magnetic valves.Regulate the spraying system device by device specification the requirements installation of suiting measures to local conditions.Spraying rotation both arms and center mechanical device etc. wherein are installed at the center, seedbed, insert the water source, simultaneously JH-3 computer controller and seedbed detecting head are connected on request, after setting corresponding data, system will regulate spraying to the test tube seedling automatically, by the temperature and humidity that the seedbed is regulated in spraying, the test tube seedling is grown, in the hardening process under suitable condition to guarantee the hardening safety and the success of seedling.
(3) transplanting and management will carry out the matrix in seedbed before transplanting-inferior loose and routine disinfection.To add IBA with No. 1 growth-promoting media during the strawberry test-tube seedling transplanting test-tube plantlet base portion is handled, be beneficial to nursery stock and take root vigorous with growth rapidly.
(4) hardening management strawberry test-tube seedling transplanting is carried out full exposure spraying hardening behind the seedbed, manage rotation both arms and center mechanical device with JH-3 computer controller, after setting corresponding data, system will regulate spraying to the test tube seedling automatically, temperature and humidity by the seedbed is regulated in spraying makes test tube seedling blade face often keep water membrane.In the seedling different growing stages, proper as long as the basic parameter of JH--3 controller is provided with, even be in high temperature and high light so, help seedling more and fully carry out photosynthesis, not only can be not withered, take root on the contrary rapidly, it is vigorous to grow.Therefore the Rational Parameters setting of JH-3 computer controller is the key of hardening success.After facts have proved strawberry test-tube seedling transplanting seedbed, the optimal parameter of JH-3 computer controller is set to: 1. seedling is preceding 3 days, and it is 50 that basic parameter is set, and water spraying time is 70, and evaporation coefficient is 50-70, and water stopping time is 10; 2. seedling is 3-6 days, and it is 60 that basic parameter is set, and water spraying time is 45-55, and evaporation coefficient is 80-100, and water stopping time is 20; 3. basic parameter was set in 6-15 days is 70 to seedling, and water spraying time is 45-55, and evaporation coefficient is 120-150, and water stopping time is 45; Add IBA every one time 0.05% growth-promoting media of spray in 3-5 days during the hardening, also can add simultaneously and join potassium dihydrogen phosphate etc.
(5) the management strawberry test-tube plantlet during little spray through more than 10 days hardening after, growing way is vigorous day by day, move to little spray nursery thin planting and grow seedlings, and also can move into the land for growing field crops, it is smooth that the seedbed requires, the soil is fertile, adopts the micro-nozzle reason.At this moment in the nursery or the land for growing field crops install micro-spray system, adopt computer controller regulation and administration equally.During this micro-nozzle reason, the optimal parameter of JH-3 computer controller is set to: 1. basic parameter is set in earlier stage is 120 to seedling, and water spraying time is 100, and evaporation coefficient is 150-200, and water stopping time is 60; 2. in the seedling later stage, it is 140 that basic parameter is set, and water spraying time is 60, and evaporation coefficient is 200, and water stopping time is 120.During this, add 0.4% potassium dihydrogen phosphate etc. every one time 0.06% growth-promoting media of spray in 5 days.Left and right sides seedling was tending towards prosperous and powerful in common 20 days, can close the computer controller, thereby removed the automated intelligent management, and growing seedlings enters common daily management.
Claims (4)
1, a kind of test-tube plantlet full exposure spraying hardening technology, it is characterized in that: in the plant tissue culture seedlings-raising, test-tube plantlet moves to the outdoor hardening process from indoor, adopt JH-3 computer controller, spraying rotation bell machines device etc., the test tube seedling is regulated spraying, by the temperature and humidity that the seedbed is regulated in spraying, the test tube seedling is grown in the hardening process under suitable condition.
2, a kind of test-tube plantlet full exposure spraying hardening technology according to claim 1, it is characterized in that: the good level land of ventilated illumination condition is selected in the seedbed, and the seedbed is made as circle, 10-14 meters of diameters; Can be divided into 1-1.5 meters wide nursery in the seedbed, leave 0.2-0.3 meter space between the nursery is adjacent; The nursery bottom is a cobble, and medium sand or perlite that upper berth one deck is 30-40 centimetres are made medium.
To carry out once loose and routine disinfection to the matrix in seedbed before transplanting; To handle the test-tube plantlet base portion with 1,2, No. 5 growth-promoting media respectively its different seeds during test-tube seedling transplanting.
3, a kind of test-tube plantlet full exposure spraying hardening technology according to claim 1, it is characterized in that: test-tube seedling transplanting is carried out full exposure spraying hardening behind the seedbed, with JH-3 controller regulation and control spraying system device, its optimal parameter is set to: 1. seedling is preceding 8 days, it is 50 that basic parameter is set, water spraying time is 70, and evaporation coefficient is 50-70, and water stopping time is 10; 2. seedling is 8-20 days, and basic parameter is 60, and water spraying time is 45-55, and evaporation coefficient is 80-100, and water stopping time is 20; 3. seedling is 20-35 days, and basic parameter is 70, and water spraying time is 45-55, and evaporation coefficient is 120-150, and water stopping time is 45; Add IBA etc. every one time 0.5% growth-promoting media of spray in 7-10 days during this.
4, a kind of test-tube plantlet full exposure spraying hardening technology according to claim 1, it is characterized in that: test-tube plantlet is after the hardening of a wheat harvesting period, the seedling growing way is vigorous day by day, move to little spray nursery thin planting and grow seedlings, adopt JH-3 controller regulation and control spraying system device equally, its optimal parameter is set to: 1. seedling in earlier stage, basic parameter is 120, water spraying time is 100, and evaporation coefficient is 150-200, and water stopping time is 60; 2. in the seedling later stage, basic parameter is that 140. water spraying time are 60, and evaporation coefficient is 200, and water stopping time is 120; During this, every 10---one time 0.6% growth-promoting media of spray in 15 days adds 0.4% potassium dihydrogen phosphate etc.; Seedling is tending towards prosperous and powerful after common 40 days, can close the computer controller, and growing seedlings enters common daily management.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103460971A (en) * | 2013-09-30 | 2013-12-25 | 湖南省农业生物资源利用研究所 | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings |
| CN105409742A (en) * | 2015-11-13 | 2016-03-23 | 周福进 | Method using nutrient media to cultivate strawberries |
| CN107409672A (en) * | 2017-05-05 | 2017-12-01 | 中国农业科学院茶叶研究所 | A kind of tea tree fast seedling-cultivating method of full exposure spraying culture |
| CN109757233A (en) * | 2019-03-18 | 2019-05-17 | 中国林业科学研究院亚热带林业研究所 | A kind of Cluster Bamboo full exposure is sprayed cuttage rapid propagating method |
| CN111642313A (en) * | 2020-06-15 | 2020-09-11 | 上饶市林业科学研究所 | Method for cultivating strong seedlings of phoebe bournei in open field in container |
-
2008
- 2008-08-09 CN CNA2008100320790A patent/CN101496497A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103460971A (en) * | 2013-09-30 | 2013-12-25 | 湖南省农业生物资源利用研究所 | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings |
| CN105409742A (en) * | 2015-11-13 | 2016-03-23 | 周福进 | Method using nutrient media to cultivate strawberries |
| CN107409672A (en) * | 2017-05-05 | 2017-12-01 | 中国农业科学院茶叶研究所 | A kind of tea tree fast seedling-cultivating method of full exposure spraying culture |
| CN109757233A (en) * | 2019-03-18 | 2019-05-17 | 中国林业科学研究院亚热带林业研究所 | A kind of Cluster Bamboo full exposure is sprayed cuttage rapid propagating method |
| CN111642313A (en) * | 2020-06-15 | 2020-09-11 | 上饶市林业科学研究所 | Method for cultivating strong seedlings of phoebe bournei in open field in container |
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Open date: 20090805 |