CN101492695A - Process for producing sumach extract by using aspergillus fermentation and sumach extract produced by using the process - Google Patents
Process for producing sumach extract by using aspergillus fermentation and sumach extract produced by using the process Download PDFInfo
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- CN101492695A CN101492695A CNA2008100045539A CN200810004553A CN101492695A CN 101492695 A CN101492695 A CN 101492695A CN A2008100045539 A CNA2008100045539 A CN A2008100045539A CN 200810004553 A CN200810004553 A CN 200810004553A CN 101492695 A CN101492695 A CN 101492695A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The method relates to a method for preparing a sumac extract by adopting aspergillus fermentation of aspergillus oryzae and the like and the sumac extract prepared by the method, in particular to the method for preparing the sumac extract with the following steps: a) the sumac is dried, grinded and water is added to carry out moisture absorption on the sumac; b) hygroscopic material prepared in step a) is cooked; c) aspergillus is inoculated, nurtured and fermented in the cooked material prepared in step b), the aspergillus is selected from a group composed of aspergillus oryzae, aspergillus kawachii, aspergillus usamii, aspergillus shirousamii, aspergillus niger and aspergillus awamori; d) water is added to leavening prepared in step c) and is heated; e) liquid ingredients are recovered from the heated material prepared in step d). The method for preparing the sumac extract of the invention sharply improves efficiency of numerous active ingredients of the sumac, eliminates toxic ingredients thereof and improves functional characteristics of the prepared sumac extract.
Description
Technical field
The lacquer tree extract that the present invention relates to the lacquer tree preparation method of extract and use the preparation of this method especially uses the lacquer tree method for preparing extractive of the fermentation of aspergillus oryzae (Aspergillus oryzae), Aspergillus albicans aspergillus such as (aspergillus kawachii).
Background technology
Lacquer tree belongs to the deciduous tree of Anacardiaceae, often grows in the hill of south east asia such as China that comprises Korea S and Japan.Sever the bark of above-mentioned lacquer tree, then discharge the resin (lacquer) in the secondary sieve part tissue that is present in bark, this lacquer is as having the also good natural paint of stay-in-grade of resistance and weather resistance and life-time service, especially in Korea S, Japan and China etc. are widely used in painted basin jar (lacquerware) etc.Especially compare with at present widely used fall family and enamel, painted Korea S mother-of-pearl lacquer has obtained the approval of one's best quality in the whole world.
And, owing to the plurality of active ingredients that contains in a large number in the above-mentioned lacquer tree the human body beneficial, so begin to be widely used in medicinal material and the food in the past.To also produce effect (Compendium of Materia Medica, this agrostology) such as gastro-enteritis, heart trouble, sacroiliitis, hypertension, diabetes, apoplexy and confirmed fatigues, spreading as the traditional food that uses lacquer tree has lacquer chicken and lacquer duck etc., this is because it can decompose the harmful fatty substance that comprises in chicken or the duck, and can also utilize the effective constituent of lacquer itself, therefore now also be subjected to liking of people.
Composition as lacquer tree has the laccol based compound, doubly acid (gallic acid) and other multiple materials to be confirmed of the flavonoid compound of fisetin, fustin, butein, sulphuretin, Quercetin etc. and phenolic compound system, (the mountain forest Rooms such as its main drug effect has anticancer, anti-oxidant and alleviation is still drank after a night, Forest Genetics Research Inst., Luo Tian turns round and look at (transliteration) research group, 997.4.22, Food science proceedings, vol 31,238-245).
As above-mentioned, lacquer tree has the multiple material to the human body beneficial, still, as the method for extracting these materials, only uses the simple method that lacquer tree is boiled in water, does not also work out the more method of the composition of validity ground extraction lacquer tree.
In addition, as above-mentioned, the multiple pharmacological effect of lacquer tree still also can't freely be used at present by known, and this is because of the allergic conditions to human body, promptly so-called " lacquer rash " phenomenon.Cause that the main component in the lacquer tree of " lacquer rash " symptom is a laccol.Laccol is flaxen liquid, is dissolved in water, alcohol or the ether, is to have the unsaturated compound (Merck index 9697) that side chain R base contains the chemical structure of the individual dual combination of carbon 15-17.Because the allergic symptom as above-mentioned lacquer tree is judged as the taboo material that can't eat in hospital of Dermatology Department.On the other hand, carrying out multiple relevant anaphylactoid treatment problem abroad, but the country that almost is not used to eat as Korea S.
Thus, in order actively to use the pharmacological effect of lacquer tree, press for the method for removing the coating removal poison when extracting the lacquer tree composition expeditiously.Thereby carrying out removing the toxic multiple research of lacquer tree.
For example, in korean patent application 1994-266 number, disclose bark with the exsiccant lacquer tree be placed on pine needle, White Mulberry Root-bark, tribute fructus amomi boil 9-11 hour in the water after, remove insoluble substance, thereby obtain the method for removing toxic lacquer tree extract.And, a kind of like this method of removing the lacquer poison in the lacquer tree extract is disclosed in korean patent application 1999-56704 number, this method comprises: with lacquer tree warm air drying step more than 4 hours in 170 ℃; Utilize water, in 105-200 ℃, extract 1～24 hour step; And extract filtered and spissated step.In korean patent application 2001-28569 number, disclose and lacquer tree etc. is put into the process furnace that reflux and breather are installed with water carried out 2 hours ventilation, heated simultaneously, thus the technical scheme of removing laccol.And, in korean patent application 2000-35434 number, disclose and comprised that (a) utilizes after steam steams sumac(h)bark 90 seconds, be immersed in to take out and put driedly with back in the washing water of rice of glutinous rice preparation, this process is repeated 1 kilogram in the lacquer that 3 times method handles; (b) mix chestnut tree barks with 3: 1 weight and mulberry tree bark (White Mulberry Root-bark) back fries out with little fire, mix with 18 liters water ratio, it is decocted to capacity reduce to half, 9 liters of the additives by this method acquisition with the 500g that fries out material; (c) mix 9 liters water in the additive with the above-mentioned processing of the lacquer of 1 kilogram above-mentioned processing and 9 liters, obtain the step of 18 liters mixture; And, said mixture is decocted the toxic method of removing coating removal liquid that 3 hours step is a feature.In korean patent application 2000-31328 number, disclose in 100～240 ℃ high temperature directly/carried out indirectly after 10～50 minutes the heat treated, utilized the method for organic solvent extraction lacquer trees such as water or alcohol.But these methods or need carry out harsh heat treatment step, perhaps residual harmful organic solvent, and be not particularly related to the highly effective extraction method of the effective constituent in the lacquer tree.
For this reason, in order to extract the effective constituent of lacquer tree expeditiously, thereby develop fine lacquer tree extract more, inventor of the present invention is through discovering after the boiling lacquer tree aspergillus such as inoculation aspergillus oryzae, Aspergillus albicans, and fermentation back preparation lacquer tree extract, then can extract the plurality of active ingredients that contains in the lacquer tree in a large number, can also improve the sense feature of the lacquer tree extract of preparation with gentle more condition, and can remove the toxic component of lacquer tree, thereby finish this invention.
Summary of the invention
The lacquer tree extract that the object of the present invention is to provide a kind of lacquer tree preparation method of extract and prepare by this method, this method is utilized the fermentation of aspergillus such as aspergillus oryzae, the various effective constituents of high efficiency extraction lacquer tree, improve the functionality feature of prepared lacquer tree extract, and can remove the toxic component of lacquer tree.
In order to achieve the above object, the invention provides a kind of lacquer tree method for preparing extractive that utilizes fermentation, this method comprises:
A) with the lacquer tree crushed after being dried, add water, make its moisture absorption;
B) hydroscopic substance that in above-mentioned a) step, prepares of boiling;
C) at above-mentioned b) inoculation and cultivate the aspergillus of selecting among the group who constitutes by aspergillus oryzae, Aspergillus albicans, Aspergillus usamii (Aspergillus usamii), aspergillus shirousamii, aspergillus niger (Aspergillus niger) and Aspergillus awamori (Aspergillus awamori) in the cooking material of step preparation, and make its fermentation;
D) at above-mentioned c) place after adding water in the fermented product for preparing in the step, heat again afterwards; And
E) from above-mentioned d) recovering liquid composition the heating thing of step preparation.
And, the invention provides a kind of method, it is characterized in that, in above-mentioned a) step, in the lacquer tree crushed material of 100 weight parts, add starchy materials such as the fine flour of 0.1～100 weight part or rice and flour.
And the present invention also provides a kind of method, it is characterized in that, adds the above-mentioned c of 0.1～0.3 weight part with respect to the lacquer tree crushed material of 100 weight parts) aspergillus of step.
And, the invention provides a kind of method, it is characterized in that above-mentioned c) and the aspergillus of step is aspergillus oryzae.
And the present invention also provides the lacquer tree extract according to method for preparing.
And the present invention also provides the step of the lacquer tree crushed material of frying drying and crushing.By this step, can remove the toxicity of coating removal more fully.
Below, illustrate step by step according to lacquer tree preparation method of extract of the present invention.
Drying step
In order to prepare lacquer tree extract according to utilization fermentation of the present invention, at first cut down lacquer tree, separate branch and the stem of lacquer tree afterwards, and carry out drying.Relate to lacquer tree of the present invention and be not limited to the place of production and kind.Can use raw lacquer tree, still, after pulverising step in, have difficulty when handling because of the viscosity of lacquer, therefore preferably raw lacquer is set and is carried out drying.Can adopt natural seasoning, hot air seasoning or freeze-drying etc. as the desiccating method in this step, but the preferred natural seasoning that adopts.In above-mentioned seasoning, when sun was done, perhaps drying effect was fine, but the ultraviolet ray of the sun might be decomposed effective constituent, therefore, preferably dries in the shade.
Pulverising step
To in above-mentioned steps, be ground into the sawdust shape by the exsiccant lacquer tree.The pulverizing of this lacquer tree can be undertaken by known method, especially utilizes known machine to carry out.Example as above-mentioned pulverizing machine comprises rod mill (pin crusher) and hammer mill (hammer crusher) etc.In an embodiment of the present invention, after the use rod mill is pulverized, utilize the sieve of 8-mesh to reclaim the crushed material of the lacquer tree of certain size.
The moisture absorption step
Add water in the crushed material of the lacquer tree that in above-mentioned steps, prepares, make its moisture absorption.The purpose of this step is to improve the cooking effect in the following boiling step.To achieve these goals, preferably the lacquer tree crushed material of 100 weight parts is added the water of 30～40 weight parts.And, in order fully to absorb moisture, in the lacquer tree crushed material, add after the water, preferably placed 30～60 minutes.
In this step, in order to improve the hobby of the finished product, and improve the extraction efficiency of the plurality of active ingredients of lacquer tree, except above-mentioned water, can also add starchy materials such as fine flour or rice and flour.Can add above-mentioned starchiness than the ratio of 0.1～100 weight part with the lacquer tree crushed material of 100 weight parts.
The boiling step
The crushed material for preparing in above-mentioned steps and the mixture of water are carried out boiling.The purpose of this step is to kill the various bacteriums in the said mixture, in the fermentation step below, makes zymogenic growth maximization, thereby improves the efficient of fermentation.The boiling of this step can be passed through usual method, under atmospheric pressure, the crushed material of above-mentioned lacquer tree and the mixture of water is heated.And, can also be undertaken by the pressure cooking method of using pressure cooking pot etc.When the being atmospheric cooking about 60 minutes time of boiling, during the 1.3kg pressure cooking about 20 minutes.
Fermentation step
To in above-mentioned steps, cool off by the cooking material of boiling.Cooling temperature is preferably 35 ℃.In above-mentioned cooking material, inoculate aspergillus afterwards,, be preferably under 30 ℃ and ferment at 25-35 ℃.Leavening temperature is low excessively, and it is not ideal enough then to ferment, and leavening temperature is too high, and then assorted bacterium breeding such as Bacillus subtilus (bacillus subtilis) influences the quality of product.
Above-mentioned aspergillus is preferably from the group that is made of aspergillus oryzae, Aspergillus albicans, Aspergillus usamii, aspergillusshirousamii, aspergillus niger and Aspergillus awamori and selects more than one, most preferably is aspergillus oryzae.Preferably add the above-mentioned aspergillus of 0.1～0.3 weight part for the lacquer tree crushed material of 100 weight parts.
Fermentation time is preferably 30～40 hours, most preferably is 35 hours.Fermentation time is too short, then toxic component remove with the ejection of effective constituent thoroughly inadequately, fermentation time is too long, the effective constituent that has then sprayed is impaired because of the autodigestion of aspergillus.
Maturation stage
Make it static after adding water in the lacquer tree fermented product that in above-mentioned steps, prepares, thereby make the fermented product slaking of above-mentioned lacquer tree.The purpose of this step is to make the multiple ferment that produces during the fermentation to act on lacquer tree, thereby sprays the effective constituent that is contained in the microtexture more effectively.Specifically, in above-mentioned lacquer tree fermented product, add the water of 2～3 times of weight of above-mentioned lacquer tree fermented product,, be preferably and make its static 1～5 hour under 55 ℃, be preferably 2～3 hours at 50～60 ℃.The amount of the water of above-mentioned interpolation according to the concentration of the final lacquer tree extract of hope, can be adjusted, and said temperature can be adjusted according to above-mentioned rest time.
Heating steps
The fermented product of the lacquer tree of slaking in above-mentioned steps and the mixture of water are heated, boil.The purpose of this step is to extract the effective constituent that remains in the above-mentioned lacquer tree fermented product, eliminates aspergillus and the ferment that remains in the above-mentioned lacquer tree fermented product simultaneously, thereby improves the quality of the lacquer tree extract of final production, keeping quality and hobby property.The above-mentioned time of boiling is preferably 1～5 hour.
Recycling step
From the raw material of above-mentioned slaking and heating, removing the solids components such as powder of lacquer tree, recovering liquid composition, thereby preparation lacquer tree extract.This recycling step can use center of circle separator or squeezing machine etc. to carry out usually.
In an embodiment of the present invention, the method according to this invention, use aspergillus of the present invention such as aspergillus oryzae, the fermentation lacquer tree, prepared the lacquer tree extract, analyzed the extract that is present in the lacquer tree in the prepared lacquer tree extract, and with the result that the lacquer tree extract (comparative example 1) of usual method preparation compares, can confirm that its extraction efficiency brings up to 130～145%; And can confirm that when above-mentioned fermentation, when adding as starchiness such as fine flours, its extraction efficiency significantly brought up to for 245～287% (with reference to table 1).And, in a test example of the present invention, to the alleviation effect of being still drank after a night of one of representational pharmacological action of lacquer tree, the reduction effect of determining alcohol in the blood of the lacquer tree extract of prepared according to the methods of the invention lacquer tree extract and usual method preparation is compared (with reference to test example 4).Its result, prepared according to the methods of the invention lacquer tree extract is compared with the control group that drops into normal saline solution, significantly reduced the concentration of the alcohol in the blood (alcohol), thereby can confirm that it has the effect of being still drank after a night of alleviation, and, prepared according to the methods of the invention lacquer tree extract is compared (comparative example 1) with the lacquer tree extract of usual method preparation, effect with determining alcohol in the higher reduction blood, thereby, can extract the various effective constituents (with reference to table 3) that are included in the lacquer tree effectively according to the present invention.
In a test example of the present invention, confirmed whether there is toxicity in prepared according to the methods of the invention lacquer tree extract and the lacquer tree extract with the usual method preparation.Its result has anaphylaxis with the lacquer tree extract of usual method preparation to skin, still, prepared according to the methods of the invention lacquer tree extract to skin without any reaction (with reference to test example 1).And, to prepared according to the methods of the invention lacquer tree extract, use high performance liquid chromatography (HPLC) and vapor-phase chromatography (GC), confirmed whether there is laccol, its result fails to detect laccol (with reference to Fig. 2 b and Fig. 3 b) from prepared according to the methods of the invention lacquer tree extract.Can know that from above-mentioned result the method according to this invention can remove the toxic laccols of lacquer tree such as showing the lacquer rash effectively, can prepare does not have toxic lacquer tree extract.
In another test example of the present invention, carried out organoleptic test about taste and fragrance to prepared according to the methods of the invention lacquer tree extract and with the lacquer tree extract of usual method preparation.Its result compares with the lacquer tree extract for preparing with usual method, and the functionality of prepared according to the methods of the invention lacquer tree extract is good (with reference to table 2).Can confirm that from above-mentioned result prepared according to the methods of the invention lacquer tree extract can improve functionality such as the taste of lacquer tree extract and fragrance.
Description of drawings
Fig. 1 is for illustrating the lacquer tree method for preparing extractive that utilizes the fermentation of aspergillus such as aspergillus oryzae according to of the present invention stage by stage;
Fig. 2 is the HPLC analytical results that the toxicant (being laccol) that does not detect lacquer tree from prepared according to the methods of the invention lacquer tree extract is shown;
A: laccol reference material
B: the lacquer tree extract of the embodiment of the invention 1
Fig. 3 is the GC-MSD analytical results that the toxicant (being laccol) that does not detect lacquer tree from prepared according to the methods of the invention lacquer tree extract is shown;
A: laccol reference material
B: the lacquer tree extract of the embodiment of the invention 1
Embodiment
Below, according to embodiment the present invention is described in further detail.But these embodiment are used for illustrating in further detail the present invention, and scope of the present invention is not limited to these embodiment.
Embodiment 1
Utilize the preparation of the lacquer tree extract of aspergillus oryzae fermentation
After in the cool the branch of lacquer tree and stem being carried out drying, utilize rod mill to pulverize, filter out the crushed material of certain size with the sieve of 8mesh.The water that adds 350g in the above-mentioned crushed material of 1kg makes its even absorption, pushes, and places to be placed in the food steamer boiling after 1 hour 40 minutes, and boils in a covered pot over a slow fire 20 minutes.These taking-ups are cooled to 35 ℃, here inoculate after the aspergillus oryzae class bacterium 1.5g, be contained in the container, utilize thermostat container (incubator), 30 ℃ of bottom fermentations 35 hours with the thickness of 2cm.In above-mentioned fermented product, add the water of 2L, 55 ℃ of following slakings 2 hours, after boiling 1 hour, squeeze, thereby the lacquer tree extract of 1.5L of preparation has obtained to be equivalent to 2.49% evaporation drying material of raw material to found that of studying of the lacquer tree extract of preparation.Compare with the comparative example 1 that extracts by usual method, increased income about 145% composition (with reference to table 1).
Embodiment 2～4
Utilize other aspergillus family preparations according to lacquer tree extract of the present invention
Aspergillus as fermentation step, replace outside the aspergillus oryzae with Aspergillus albicans, Aspergillus usamii and aspergillusshirousamii, other methods identical with embodiment 1 have prepared the lacquer tree extract of embodiment 2 to embodiment 4, to found that prepared lacquer tree extract is analyzed, obtained respectively with respect to 2.25% of raw material, 2.32%, 2.45% evaporation drying material.Compare with common extracting method (comparative example 1), increased income the composition (with reference to table 1) more than 131%, 135%, 142% respectively.
Embodiment 5
Add the starchiness preparation according to lacquer tree extract of the present invention
When in the above-mentioned crushed material of 1kg, adding the water of 350g, add the fine flour (wheat flour) of 10g, in addition, other methods identical with the foregoing description 1 have prepared the lacquer tree extract, to found that prepared lacquer tree extract is analyzed, obtained 4.22% evaporation drying thing, and compared, obtained the outstanding achievement (with reference to table 1) more than 245% with usual method (comparative example 1) with respect to raw material.
Embodiment 6～8
Add the starchiness preparation according to lacquer tree extract of the present invention
When in the above-mentioned crushed material of 1kg, adding the water of 350g, add the fine flour (wheat flour) of 10g, and the aspergillus of fermentation step, replace aspergillus oryzae with Aspergillus albicans, Aspergillus usamii and aspergillus shirousamii, in addition, identical with the foregoing description 1 method has prepared the lacquer tree extract.Analyze found that of prepared lacquer tree extract, obtained with respect to 4.95%, 4.55% of raw material, 4.30% evaporation drying thing is compared with usual method (comparative example 1), has obtained 288% respectively, 265%, 250% the extraordinary achievement (with reference to table 1) that increases income.
Comparative example 1
Do not utilize the preparation of the lacquer tree extract of fermentation
In the crushed material of the branch of the lacquer tree of 1kg and stem, add the water of 2350g, after boiling 1 hour, squeeze, thereby prepared the lacquer tree extract,, obtained 1.72% evaporation drying thing with respect to raw material to found that it is analyzed.
Table 1
| Raw material ratio extracted amount (dry substance amount) (%) | The rate that increases income (%) of the extract of the embodiment method of comparing with the method for comparative example 1 | |
| Comparative example 1 | 1.72 | 100 |
| Embodiment 1 | 2.49 | 145 |
| Embodiment 2 | 2.25 | 131 |
| Embodiment 3 | 2.32 | 135 |
| Embodiment 4 | 2.45 | 142 |
| Embodiment 5 | 4.22 | 245 |
| Embodiment 6 | 4.95 | 288 |
| Embodiment 7 | 4.55 | 265 |
| Embodiment 8 | 4.30 | 250 |
Test example 1
The lacquer tree extract of embodiment and comparative example is to the irritant test of skin
Confirmed the toxicity of the lacquer tree extract of the lacquer tree extract of the foregoing description 1 to embodiment 8 and above-mentioned comparative example 1.Each lacquer tree extract of above-mentioned preparation was coated on 5 men and women's the skin (arm) of susceptibility physique, observed whether reflection is arranged, observed altogether 5 days.
Its result has reflection according to the lacquer tree extract of the comparative example 1 of usual method preparation to 5 all skins, but the lacquer tree extract of embodiments of the invention 1 to embodiment 8, to skin without any reflection.Can confirm that according to The above results prepared according to the methods of the invention lacquer tree extract has been removed toxic component, i.e. laccol fully.
Test example 2
Use in the lacquer tree extract of machine analysis according to present method preparation and whether comprise laccol
In order whether to have laccol in the lacquer tree extract of confirming the embodiment of the invention 1, adopt methods (" Atmosperic pressure ionization LC-MS-MSdetermination of Urusiol cogenders " such as W.M.Draper, J.Agric.Food Chem.2002,50,1852-1858), having carried out HPLC and GC analyzes.The laccol of standard filters with acetone extraction lacquer tree liquid, the dry thing that obtains behind the drying under reduced pressure afterwards.
The treatment process of test portion is: with the methylene dichloride (dichloromethane) of 50ml the lacquer tree extract of the embodiments of the invention 1 of 50ml is carried out 3 times and extract, reclaim organic layer, behind the drying under reduced pressure, be filled to florisil post (florisil column), spray by methylene dichloride, reclaim ejecta afterwards, it is carried out drying under reduced pressure.After residue being dissolved in the hexane (hexane) of 20ml, divide with acetonitrile (acetonitrile) solvent, the acetonitrile layer that reclaims is carried out drying under reduced pressure, dissolving once more in the acetonitrile of 1ml afterwards, under following condition, carry out HPLC and analyze, show its result at Fig. 2.
The HPLC analysis condition
Post: C18 capcellpak (4.6 * 250nm, 5 μ m)
Wavelength: 276nm
Ejection solvent: 30% methyl alcohol → 100% methyl alcohol (50 minutes)
Flow velocity: 0.5ml/min
And, take out the above-mentioned acetonitrile solution of 100 μ l, with the BSTFA of 100 μ l, after the pyridine of 50 μ l (pyridine) mixes,, carry out GC-MSD below under the condition and analyze 80 ℃ of heating 30 minutes down, show its result at Fig. 3.
The GC-MSD analysis condition
Post: DB-5MS capillary column (30m * 0.25mm I.D. * 0.25 μ m df)
Injection form: clastotype (split mode) (separation rate 10: 1)
Column temperature: 15 ℃/minute of 50 ℃ (5 minutes) → 250 ℃ (15 minutes) at
Detector: 5973MSD
Implantation temperature: 225 ℃
Ion source temperature: 230 ℃
As shown in Figure 2, the laccol of standard in HPLC analyzes, the residence time show 48.88 minutes, 51.25 minutes, 54.72 timesharing the peak (Fig. 2 a), but do not detect laccol (Fig. 2 b) in the lacquer tree extract according to embodiments of the invention 1.And, as shown in Figure 3, in GC-MSD analyzes, the laccol of standard 25.08 minutes residence times, 25.42 minutes, 26.81 timesharing demonstrate the peak (Fig. 3 a), but do not detect laccol (Fig. 3 b) in the lacquer tree extract according to the embodiment 1 of present method.
Test example 3
Detect the sense of the lacquer tree extract of embodiment and comparative example
The lacquer tree extract of prepared according to the methods of the invention embodiment 1 to embodiment 8 and the lacquer tree extract of the comparative example 1 of preparation have usually been carried out the organoleptic test of relevant taste and fragrance.
By trained 15 personnel, carried out the organoleptic test with 4 integral body preference degree.Average score for 15 organoleptic test personnel of 4.0 full marks has been shown in the table 1.Mark is high more, and the expression functionality is good more.
Above-mentioned organoleptic test's result has been shown in table 2.As shown in table 2, the functionality of the lacquer tree extract of embodiment 1 to embodiment 8 all is higher than the lacquer tree extract of comparative example 1.Can confirm that from above-mentioned result compare with the lacquer tree extract for preparing according to usual method, prepared according to the methods of the invention lacquer tree extract has better functionality.
Table 2
| Classification | Preference degree |
| Comparative example 1 | 1.13 |
| Embodiment 1 | 3.80 |
| Embodiment 2 | 3.13 |
| Embodiment 3 | 2.20 |
| Embodiment 4 | 3.07 |
| Embodiment 5 | 3.97 |
| Embodiment 6 | 3.50 |
| Embodiment 7 | 2.70 |
| Embodiment 8 | 3.30 |
Test example 4
The alleviation of comparing embodiment and the comparative example effect of being still drank after a night
The reduction effect of the alcohol in the blood of the lacquer tree extract of the lacquer tree extract by observing prepared according to the methods of the invention embodiment 1 and embodiment 5 and the comparative example 1 of usual method preparation is relatively alleviated the effect of being still drank after a night.Laboratory animal has been used the lab mice (sprague-dawley) (male) in 6 weeks, carries out the duration of test room temp and remains on 25 ℃, can freely take in foodstuff, has freely supplied with distilled water as tap water.Laboratory animal is divided into 4 groups, every group 5, drop into 25% ethanol (ethanol) of 10ml after jejunitas 24 hours to stomach and intestine with probe, drop into the normal saline solution of 30ml after 10 minutes to comparative group, drop into the lacquer tree extract of the embodiment 1 of 30ml, the lacquer tree extract of embodiment 5, the lacquer tree extract of comparative example 1 to remaining three groups of difference per os.Drop into after 1 hour behind the alcohol, take the blood of 3ml, isolate serum, utilize NAD-ADH to analyze (sigma) pure detector of bottle (assay vial) by the center of circle from animal, the concentration of the alcohol in the detection blood, its result is illustrated among Fig. 3.As shown in Figure 3, compare with the comparative group that drops into normal saline solution, the concentration of the alcohol in the blood of the group of the lacquer tree extract of input embodiment 1 and embodiment 5 is low, significantly reduced the concentration of the alcohol in the blood, thereby can confirm to alleviate the effect of being still drank after a night, and compare with the lacquer tree extract of the comparative example 1 of usual method preparation, the effect that also has determining alcohol in the higher reduction blood, thereby in the time of can confirming that the method according to this invention prepares, can have the effective constituent of alleviating the effect of being still drank after a night from a large amount of extraction of lacquer tree.
Table 3
| Determining alcohol in the blood (mg/dl) | |
| Comparative group | 198±5 |
| The lacquer tree extract of embodiment 1 prepared in accordance with the present invention | 147±3 |
| The lacquer tree extract of embodiment 5 prepared in accordance with the present invention | 131±2 |
| Lacquer tree extract according to the comparative example 1 of usual method preparation | 168±7 |
The effect of invention
As above-mentioned, lacquer tree method for preparing extractive of the present invention not only can be removed the laccol as the toxic component of lacquer tree, can also be from the various active ingredients of lacquer tree high efficiency extraction, and the functionality features such as the taste of the lacquer tree extract of raising preparation and fragrance.
Claims (5)
1. lacquer tree preparation method of extract may further comprise the steps:
A) lacquer tree is carried out drying and pulverizing, and interpolation water makes its moisture absorption;
B) hydroscopic substance that in described a) step, prepares of boiling;
C) at described b) inoculation and cultivate aspergillus in the cooking material for preparing in the step, and to make its fermentation, described aspergillus be from by aspergillus oryzae, Aspergillus albicans, Aspergillus usamii, selects in the group of aspergillus shirousamii, aspergillus niger and Aspergillus awamori formation;
D) at described c) add water in the fermented product for preparing in the step and place after heat;
E) from described d) recovering liquid composition in the heating thing for preparing the step.
2. method according to claim 1 is characterized in that, in described a) step, adds the fine flour or the rice and flour of 0.1～100 weight part with respect to the lacquer tree crushed material of 100 weight parts.
3. method according to claim 2 is characterized in that, the ratio that adds 0.1～0.3 weight part with the lacquer tree crushed material of 100 weight parts adds described c) aspergillus of step.
4. method according to claim 3 is characterized in that, described c) aspergillus in the step is aspergillus oryzae.
5. lacquer tree extract, it prepares according to the either party's method in the claim 1 to 4.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110062584A (en) * | 2016-10-07 | 2019-07-26 | 尹后男 | The preparation method of non-toxic paint solution and using its ferment rice bran powder and powder cereal nutriment preparation method |
| CN113208968A (en) * | 2021-05-24 | 2021-08-06 | 杭州诺诚洗涤科技有限公司 | Whitening and moisturizing essence and preparation method thereof |
| CN113318032A (en) * | 2021-06-21 | 2021-08-31 | 杭州诺诚洗涤科技有限公司 | Anti-aging moisturizing cosmetic and preparation method thereof |
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| JP2738908B2 (en) * | 1994-01-20 | 1998-04-08 | 保芦 将人 | Antiviral powder material and antiviral extract |
| KR100257448B1 (en) * | 1997-08-01 | 2000-07-01 | 박홍락 | Rhus verniciflua extract having the activity of anti-cancer, differentiation induction, anti-angiogenesis, anti-oxidation and ethanol intoxification, process for the preparation thereof, and composition containig same |
| FR2796549B1 (en) * | 1999-07-19 | 2001-11-09 | Serobiologiques Lab Sa | USE OF AT LEAST ONE EXTRACT FROM A LANNEA GENUS IN A COSMETIC OR DERMOPHARMACEUTICAL COMPOSITION |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110062584A (en) * | 2016-10-07 | 2019-07-26 | 尹后男 | The preparation method of non-toxic paint solution and using its ferment rice bran powder and powder cereal nutriment preparation method |
| CN113208968A (en) * | 2021-05-24 | 2021-08-06 | 杭州诺诚洗涤科技有限公司 | Whitening and moisturizing essence and preparation method thereof |
| CN113208968B (en) * | 2021-05-24 | 2022-04-22 | 北京美丽臻颜化妆品有限公司 | Whitening and moisturizing essence and preparation method thereof |
| CN113318032A (en) * | 2021-06-21 | 2021-08-31 | 杭州诺诚洗涤科技有限公司 | Anti-aging moisturizing cosmetic and preparation method thereof |
| CN113318032B (en) * | 2021-06-21 | 2022-04-15 | 珠海世纪康美生物科技有限公司 | Anti-aging moisturizing cosmetic and preparation method thereof |
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