CN101445567A - Method for extracting platycodon polysaccharides with pressurized hot water - Google Patents
Method for extracting platycodon polysaccharides with pressurized hot water Download PDFInfo
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- CN101445567A CN101445567A CNA2008102495094A CN200810249509A CN101445567A CN 101445567 A CN101445567 A CN 101445567A CN A2008102495094 A CNA2008102495094 A CN A2008102495094A CN 200810249509 A CN200810249509 A CN 200810249509A CN 101445567 A CN101445567 A CN 101445567A
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- hot water
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- platycodon
- platycodon root
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- Polysaccharides And Polysaccharide Derivatives (AREA)
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Abstract
The invention provides a method for extracting platycodon polysaccharides with pressurized hot water, which is characterized in that the method comprises the following steps: (1) mixing Platycodon grandiflorum powder and deionized water at a mass ratio of 1:(60-120), and soaking for 5-10 min to obtain a mixed solution; (2) adding the mixed solution to a pressurized hot water device with a preheated temperature of 120-160 DEG C, and adjusting the pressure of the device to 0.45-5.60 Mpa; (3) extracting at a stirring speed of 200-300rpm for 30-60 min, releasing pressure of the pressurized hot water device, and cooling to obtain an extractive solution containing platycodon polysaccharides; and (4) filtering the extractive solution, concentrating, and lyophilizing to obtain platycodon polysaccharides. Compared with the prior art, the method uses deionized water which is harmless to human body and cheap for soaking and extraction, and has the advantages of simple process, simple production procedures, easy operation, applicability to industrial production, high extraction rate and no pollution.
Description
Technical field
The invention provides a kind of method of using pressurized hot water to extract platycodon root polysaccharide, belong to technical field of agricultural product process.
Background technology
Balloonflower root is one of main traditional Chinese medicine of China, and it is fully developed as yet, the crust of balloonflower root particularly, cauline leaf etc.Contain protein 3.5g, fatty 1.2g, carbohydrate 18.2g, calcium 260mg, phosphorus 40mg, iron 13mg, carotene 2.2mg, vitamins C 10mg, VitB1 0.45mg, riboflavin 0.44mg, food fibre 3.2g in the bright balloonflower root of every 100g.Polysaccharide is the main pharmaceutical component of balloonflower root in the balloonflower root, has reducing blood-fat, anti-inflammatory, eliminates the phlegm, effect such as antibechic.Its extracting method mainly is the solvent extraction, though that technology is stablized is feasible, impurity such as low, the consuming time length of efficient, pigment are many, operation is loaded down with trivial details, the reagent consumption is big, solvent is easily residual, environment and human body are had certain infringement.Though the method that acidolysis alkali is carried has certain effect, yet concentration is excessive, etching apparatus reduced the length of service of equipment, and concentration is low excessively, poor effect.Resin method treatment capacity is low, and length consuming time, and solvent consumption is big.
Summary of the invention
The purpose of this invention is to provide and a kind ofly can overcome above-mentioned defective, and the yield height, the time is short, solvent consumption is few, pressurized hot water simple to operate, that cost is low, environmental pollution is little extracts platycodon root polysaccharide technology.Its technical scheme is:
A kind of method of using pressurized hot water to extract platycodon root polysaccharide is characterized in that adopting following steps: (1) balloonflower powder and deionized water mix immersion 5～10min with 1: 60～120 mass ratio; (2) immersion there is the mixed solution of balloonflower powder be added to and is warmed up in advance in 120～160 ℃ of pressurized hot water devices, regulate pressure at 0.45～5.60MPa; (3) after extracting 30～60min under low whipping speed 200～300rpm, slowly exit, cool off, obtain the platycodon root polysaccharide vat liquor to the pressurized hot water device; (4) with after the vat liquor filtration, concentrating,, promptly get platycodon root polysaccharide again with the concentrated solution freeze-drying.
Described utilization pressurized hot water extracts the method for platycodon root polysaccharide, and its composition of balloonflower powder can be by balloonflower root stem, root, leaf in the step (1), one or more are formed in spending, the powder that its pulverizing or grinding are obtained.
Described utilization pressurized hot water extracts the method for platycodon root polysaccharide, adopts carbon dioxide to regulate the pressure of pressurized hot water device in the step (2).
Described utilization pressurized hot water extracts the method for platycodon root polysaccharide, and the pressurized hot water device that adopts in the step (2) is with magnetic agitation or stirring arm usually.
Described utilization pressurized hot water extracts the method for platycodon root polysaccharide, and the freeze-drying equipment therefor is special vacuum food Freeze Drying Equipment in the step (4).
The present invention compared with prior art adopts deionized water to soak and extracts, and is harmless and cheap and easy to get, by the pressure and temperature organic assembling, make hot water be in liquid state all the time, well lixiviate goes out platycodon root polysaccharide, and degradeds such as pigment are compared with traditional technology, no depigmentation operation, production technique is simple, easy to operate, easily industrialization, extraction time shortens 50～70%, extraction yield raises 100～200%, and is pollution-free.
Embodiment
Embodiment 1:
Step 1: soaking 1g order number with deionized water 60mL is 10～30 balloonflower powder, soaks 5min;
Step 2: have the mixed solution of balloonflower powder to be added to immersion and be warmed up in advance in 120 ℃ of pressurized hot water devices, regulate pressure at 0.45MPa;
Step 3: after extracting 30min under the stirring rake speed 200rpm, slowly exit, cool off, obtain the platycodon root polysaccharide vat liquor to the pressurized hot water device;
Step 4: after the vat liquor filtration, concentrating,, promptly obtain platycodon root polysaccharide again with the concentrated solution freeze-drying.
Embodiment 2:
Step 1: soaking 1g order number with deionized water 120mL is 10～30 balloonflower powder, soaks 10min;
Step 2: have the mixed solution of balloonflower powder to be added to immersion and be warmed up in advance in 160 ℃ of pressurized hot water devices, regulate pressure at 5.60MPa;
Step 3: after extracting 60min under the magnetic agitation speed 300rpm, slowly exit, cool off, obtain the platycodon root polysaccharide vat liquor to the pressurized hot water device;
Step 4: after the vat liquor filtration, concentrating,, promptly obtain platycodon root polysaccharide again with the concentrated solution freeze-drying.
Through Liebermann reaction and Liebermann-Burchard reaction, determine to contain in the extract platycodon root polysaccharide; Calculate through colorimetric method for determining, the polysaccharide yield of two examples is respectively 18.8% and 24.8%.
Claims (5)
1, a kind of method of using pressurized hot water to extract platycodon root polysaccharide is characterized in that adopting following steps: (1) balloonflower powder and deionized water mix immersion 5～10min with the mass ratio of 1:60～120; (2) immersion there is the mixed solution of balloonflower powder be added to and is warmed up in advance in 120～160 ℃ of pressurized hot water devices, regulate pressure at 0.45～5.60MPa; (3) after low whipping speed 200～300rpm extracts 30～60min down, slowly exit, cool off, obtain the platycodon root polysaccharide vat liquor to the pressurized hot water device; (4) with after the vat liquor filtration, concentrating,, promptly get platycodon root polysaccharide again with the concentrated solution freeze-drying.
2, utilization pressurized hot water as claimed in claim 1 extracts the method for platycodon root polysaccharide, it is characterized in that: its composition of balloonflower powder can be by balloonflower root stem, root, leaf in the step (1), one or more are formed in spending, the powder that its pulverizing or grinding are obtained.
3, utilization pressurized hot water as claimed in claim 1 extracts the method for platycodon root polysaccharide, it is characterized in that: adopt carbon dioxide to regulate the pressure of pressurized hot water device in the step (2).
4, utilization pressurized hot water as claimed in claim 1 extracts the method for platycodon root polysaccharide, it is characterized in that: the pressurized hot water device that adopts in the step (2) is with magnetic agitation or stirring arm usually.
5, utilization pressurized hot water as claimed in claim 1 extracts the method for platycodon root polysaccharide, it is characterized in that: the freeze-drying equipment therefor is special vacuum food Freeze Drying Equipment in the step (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102495094A CN101445567B (en) | 2008-12-19 | 2008-12-19 | Method for extracting platycodon polysaccharides with pressurized hot water |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008102495094A CN101445567B (en) | 2008-12-19 | 2008-12-19 | Method for extracting platycodon polysaccharides with pressurized hot water |
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| Publication Number | Publication Date |
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| CN101445567A true CN101445567A (en) | 2009-06-03 |
| CN101445567B CN101445567B (en) | 2012-04-25 |
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| CN2008102495094A Expired - Fee Related CN101445567B (en) | 2008-12-19 | 2008-12-19 | Method for extracting platycodon polysaccharides with pressurized hot water |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101830999A (en) * | 2010-05-21 | 2010-09-15 | 王振宇 | Method for extracting platycodon root polysaccharide |
| CN102423334A (en) * | 2011-09-30 | 2012-04-25 | 淮南联合大学 | Comprehensive extraction method for platycodin and platycodon grandiflorum polysaccharide |
| CN102450527A (en) * | 2010-10-16 | 2012-05-16 | 曹帮华 | Method for removing tannin of pawpaw |
| CN102477103A (en) * | 2010-11-22 | 2012-05-30 | 中国科学院上海药物研究所 | Platycodon grandiflorum polysaccharide, and degradation product, preparation method and application thereof |
| CN114634582A (en) * | 2022-03-14 | 2022-06-17 | 山东德信拓达生物科技研究院有限公司 | Astragalus polysaccharide extraction method and application thereof in adjusting livestock immunity |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100334968C (en) * | 2004-12-24 | 2007-09-05 | 浙江大学 | Preparation method of hairgvein agrimony poly saccharide gum |
| WO2007139057A1 (en) * | 2006-05-30 | 2007-12-06 | Fuji Oil Company, Limited | Novel water-soluble polysaccharides having high stability and method of producing the same |
| CN1958575A (en) * | 2006-11-03 | 2007-05-09 | 威海清华紫光科技开发有限公司 | Method for extracting polysaccharide and allantoin from Chinese yam |
-
2008
- 2008-12-19 CN CN2008102495094A patent/CN101445567B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101830999A (en) * | 2010-05-21 | 2010-09-15 | 王振宇 | Method for extracting platycodon root polysaccharide |
| CN102450527A (en) * | 2010-10-16 | 2012-05-16 | 曹帮华 | Method for removing tannin of pawpaw |
| CN102450527B (en) * | 2010-10-16 | 2013-03-27 | 曹帮华 | Method for removing tannin of pawpaw |
| CN102477103A (en) * | 2010-11-22 | 2012-05-30 | 中国科学院上海药物研究所 | Platycodon grandiflorum polysaccharide, and degradation product, preparation method and application thereof |
| CN102477103B (en) * | 2010-11-22 | 2014-09-10 | 中国科学院上海药物研究所 | Platycodon grandiflorum polysaccharide, and degradation product, preparation method and application thereof |
| CN102423334A (en) * | 2011-09-30 | 2012-04-25 | 淮南联合大学 | Comprehensive extraction method for platycodin and platycodon grandiflorum polysaccharide |
| CN114634582A (en) * | 2022-03-14 | 2022-06-17 | 山东德信拓达生物科技研究院有限公司 | Astragalus polysaccharide extraction method and application thereof in adjusting livestock immunity |
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| Publication number | Publication date |
|---|---|
| CN101445567B (en) | 2012-04-25 |
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Owner name: I + D FLAVOURING (GUANGZHOU) CO., LTD. Effective date: 20120203 |
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Effective date of registration: 20120203 Address after: 255086 Zibo high tech Industrial Development Zone, Shandong high Chong Park, block D, room 1012 Applicant after: Shandong University of Technology Co-applicant after: I&D International Flavours & Fragrances (GZ) Co., Ltd. Address before: 255086 Zibo high tech Industrial Development Zone, Shandong high Chong Park, block D, room 1012 Applicant before: Shandong University of Technology |
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