CN101417008B - Biberberine for injection as veterinary herb medicine composite preparation and preparation technique and use thereof - Google Patents
Biberberine for injection as veterinary herb medicine composite preparation and preparation technique and use thereof Download PDFInfo
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Abstract
The invention relates to a double coptis for injection as a veterinary herbal medicine compound preparation and a preparation technique as well as an application thereof. The compound preparation is made from the following parts of medicines by weight: 1250 portions to 3750 portions of honeysuckle, 1250 portions to 3750 portions of scutellaria and 2500 portions to 7500 portions of forsythia. Sustained release accessories contain 200 portions of hydroxypropyl methylcellulose and 100 portions of sodium carboxymethylcellulose. The containing of sustained release accessories can reduce injection times for large animals and the stress response of large animals. The veterinary herbal injection preparation has the functions of antiviral, antibacterial, anti-inflammatory and antipyretic analgesic effects, as well as improving immunity and the like, and can be applied to epidemic diarrhea, transmissible gastroenteritis, swine paratyphoid, septicemia caused by various diseases, cough, dyspnea, skin cyanosis, arthrocele, lameness, swine reproduction and respiratory disturbance syndrome and cow mastitis caused by streptococcosis, haemophilus parasuis infection, contagious pleuropneumonia, swine lung plague in veterinarian clinical practice.
Description
Technical field
The present invention relates to a kind of injection compound Chinese medicinal preparation for animals, particularly as ZHUSHEYONG SHUANGHUANGLIAN and the preparation technology and the application of herbal medicine compound preparation.
Background technology
Along with the development of society and improving constantly of living standards of the people; very big variation has taken place in people's diet structure; demand to animal derived food is growing; and after China's entry into the World Trade Organization; the animal products export trade strengthens; this has brought unprecedented prosperity and development for the animal husbandry of China, and aquaculture presents the trend of scale, intensification.For ensureing livestock birds health, increase economic benefit, antibiotic is cultured the owner and is generally doubled to use, and the food-safety problems such as residue of veterinary drug that this drug dependence causes have become the social hotspots of people's common concern.
Drug residue not only can directly produce acute, chronic toxicity effect to human body, cause the increase of bacterial drug resistance, can also directly cause potential hazard by the effect of environment and food chain, even influence the development of China's aquaculture and move towards the international market to health.So seek the antibiotic succedaneum from Chinese herbal medicine, the development green cultivation becomes general cry in the industry.
'Shuang Hualian ' has only recorded SHUANGHUANGLIAN KOUFUYE in the veterinary drug standard, can not satisfy the demand of veterinary drug clinical practice far away.Though also recorded SHUANGHUANGLIAN ZHUSHEYE in " Sichuan Province's veterinary drug standard ", its quality standard controllability is relatively poor.The veterinary clinic experiment shows: ZHUSHEYONG SHUANGHUANGLIAN popularity diarrhoea, transmissible gastroenteritis, baby swine paratyphoid, various eqpidemic diseases cause septicemia, diseases such as the cough that respiratory tract disease such as streptococcicosis, pig hemophilus disease, contagious pleuropneumonia, pig lung plague cause, dyspnea, skin cyanosis, arthroncus, limping, and various infection such as pig syndrome of breeding and respiration disorders, mammitis of cow, endometritis, foot rot, pneumonia, calf diarrhea are all effective.
ZHUSHEYONG SHUANGHUANGLIAN be the Ministry of Agriculture first evaluate veterinary drug injection, first the non-terminally sterilised injectable powder that passes through.ZHUSHEYONG SHUANGHUANGLIAN be by Radix Scutellariae, Flos Lonicerae, Fructus Forsythiae three flavor medical materials through the injectable powder product that extracts, concentrates, operation such as refining, spray drying is made, wherein refining and spray drying is a critical process.Radix Scutellariae adopts acid-base method, extracts, makes with extra care the effective ingredient baicalin, and purity reaches more than 90%; Flos Lonicerae, Fructus Forsythiae adopt decoction and alcohol sedimentation technique, extract, make with extra care effective ingredient such as chlorogenic acid, phillyrin.Then with the effective ingredient dosing, spray-driedly make non-final sterilization injectable powder product.
Large-scale animal is when for example animal such as pig, cattle suffers from the serious symptom bacterial disease, and often appetite descends even is useless exhausted, and mixed feeding administration, drinking-water administration are difficult to prove effective the selection that drug administration by injection necessitates.But after the normal injection agent is injected to animal, typical situation is that originally systemic drug concentration is reduced to treatment concentration afterwards gradually far above treatment concentration, drops to invalid drug level at last, and the normal injection agent often needs 2 administrations every day, successive administration 3～5 days.In fact this mode is difficult to carry out, and animal is caused bigger stress.Some course of treatment, also there was similar situation in long stubborn disease (as mycoplasma pneumoniae of swine).Just become the task of top priority so prevent and treat the serious symptom bacterial disease with some stubborn disease, but present alternative durative action preparation is few with the antibacterials durative action preparation.Therefore, the developmental research to long-acting injection is very necessary.The use of slow releasing injection can change that this unfavorable pharmacokinetic properties in the past, replaces the high concentration of transmitting fast with more constant and effective treatment concentration.Therefore, same medicine accumulated dose has a longer release time in the injection site.The effect characteristics of injectable sustained-release preparation have: 1. reduce system toxicity, reduce the medication accumulated dose, increase therapeutic effect; 2. avoid occurring blood drug level and rise and fall, " peak valley " phenomenon is arranged, can obtain steady persistent curative effect.3. can reduce stress, saving medical expense, the raising cure rate of animal.Over nearly 20 years, antibacterials are mainly from the prolong drug half-life, and this develops on the one hand to reduce administration number of times.For example new macrolide antibiotics Roxithromycin, clarithromycin and the azithromycin of listing are all good than erythromycin bioavailability in recent years, and the half-life prolongs, and administration number of times all reduces to every day 2 times even 1 time for 3～4 times from every day.Fluoroquinolones, as half-life such as ciprofloxacin, ofloxacins about 4～6 hours, every day, required administration was 2～3 times, and the half-life such as new fluoroquinolones such as lomefloxacin, fleroxacin, Sparfloxacin and Gatifloxacin are all more than 12 hours, take medicine every day 1～2 time, so reducing medicine frequency is the important directions of development new drug and novel formulation.
The composition of the ZHUSHEYONG SHUANGHUANGLIAN compound Chinese medicinal preparation for animals of traditional routine mainly is that the water extracting alcohol hypostasis of Flos Lonicerae, Radix Scutellariae, Fructus Forsythiae is made dry powder, in use adds medical water dilution back injection and gives poultry.But not adding slow-release auxiliary material, is the slow release important directions of development new drug and novel formulation so be difficult to adapt to injection for animals field medicine frequency.
As mentioned above, prolong medicinal slow-release time, reduce the larger animal frequency injection, it is very meaningful to treatment to reduce the larger animal stress.
Summary of the invention
The object of the invention provides a kind of ZHUSHEYONG SHUANGHUANGLIAN and preparation technology and application as the herbal medicine compound preparation.This compound preparation contains slow-release auxiliary material, can reduce the larger animal frequency injection, reduces the larger animal stress.
As the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, make: Flos Lonicerae 1250-3750 part, Radix Scutellariae 1250-3750 part, Fructus Forsythiae 2500-7500 part by following parts of medicines by weight.
As the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, make: Flos Lonicerae 1250-3750 part, Radix Scutellariae 1250-3750 part, Fructus Forsythiae 2500-7500 part, slow-release auxiliary material 0-400 part by following parts of medicines by weight.
As the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, slow-release auxiliary material is 200 parts of hydroxypropyl methylcellulose.
As the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, slow-release auxiliary material is 200 parts of hydroxypropyl methylcellulose, 100 parts of sodium carboxymethyl cellulose.
As the preparation technology of the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, wherein:
Elder generation's weight batching: 2500 parts of Flos Loniceraes, 2500 parts of Radix Scutellariaes, 5000 parts of Fructus Forsythiaes, slow-release auxiliary material are 200 parts of hydroxypropyl methylcellulose, 100 parts of sodium carboxymethyl cellulose.
(1) Radix Scutellariae decocts with water three times, and collecting decoction is transferred pH to 1.0～2.0, leave standstill, filter, precipitation adds the water of 8 times of volumes, transfer pH to 7.0, add the ethanol with water volume equivalent again, stir and make dissolving, filter, filtrate is transferred pH to 2.0, and 60 ℃ are incubated 30 minutes, leave standstill, filter, precipitation is washed pH to 4.0 with ethanol, the water that adds 10 times of volumes stirs, and transfers pH to 6.0, add 0.5% active carbon, fully stir, 50 ℃ are incubated 30 minutes, add 1 times of volume of ethanol and stir, filter, filtrate is transferred pH to 2.0,60 ℃ are incubated 30 minutes, leave standstill, and filter, after precipitation is washed with small amount of ethanol, drying, standby;
(2) Flos Lonicerae, Fructus Forsythiae decoct with water secondary, filter, and it is 1.20～1.25 that filtrate is concentrated into 70～80 ℃ relative density, cooling adds ethanol, makes to contain the alcohol amount and reach 75%, fully stir, leave standstill, the leaching supernatant reclaims ethanol to there not being the alcohol flavor, the water that adds 3～4 times of volumes, transfer pH to 7.0, fully stir and be heated to and boil, leave standstill, the leaching supernatant, the relative density that is concentrated into 70～80 ℃ is 1.10～1.15, and cooling adds ethanol and makes and contain the alcohol amount and reach 85%, leave standstill, the leaching supernatant reclaims ethanol to there not being the alcohol flavor, and is standby;
(3) get Radix Scutellariae extract, it is an amount of to add water, transfers pH to 7.0, heating makes dissolving, adds Flos Lonicerae, Fructus Forsythiae extract, concentrate approximately to 80 ℃ relative density be 1.15, add 0.5% active carbon, keep little the boiling 15 minutes of pH value 7.0 heating, cooling, add slow-release auxiliary material, filter, spray drying is received powder, packing promptly gets injectable powder.
Adopted technology such as advanced and applicable extraction, purification and spray drying in this veterinary drug production process, technical maturity is stable, product effective component content height, and good stability has improved bioavailability, has reduced clinical use cost.
The preparation technology of ZHUSHEYONG SHUANGHUANGLIAN compound Chinese medicinal preparation for animals, wherein:
(3) get Radix Scutellariae extract, it is an amount of to add water, transfers pH to 7.0, heating makes dissolving, adds Flos Lonicerae, Fructus Forsythiae extract, concentrate approximately to 80 ℃ relative density be 1.15, add 0.5% active carbon, keep little the boiling 15 minutes of pH value 7.0 heating, cooling, add hydroxypropyl methylcellulose, sodium carboxymethyl cellulose, filter, powder is received in lyophilization, packing promptly gets injectable powder.All the other are with above-mentioned (1), (2) step.
Microcrystalline Cellulose, methylcellulose, hydroxypropyl methylcellulose, sodium carboxymethyl cellulose, polyvidone can form the bioerodable skeleton of drug molecule at interior multiple adjuvant.After intramuscular injection or subcutaneous low-lying area were penetrated, medicine depended on the concentration of storing free drug molecule the storehouse from the release of gel skeleton.
But which kind of adjuvant or the combination between them can better take into account the effect of therapeutic effect and slow release and need screen by experiment.
Below we have selected multiple adjuvant and the combination between them, investigate their contributions respectively, and to the influence of therapeutic effect to slow release.
Formula table
| Prescription | Medicinal ingredient | Slow-release auxiliary material |
| A1 | 5000 parts of 2500 parts of Fructus Forsythiaes of 2500 parts of Radix Scutellariaes of Flos Lonicerae | 100 parts of 200 parts of sodium carboxymethyl cellulose of hydroxypropyl methylcellulose |
| A2 | 2500 parts of Flos Loniceraes, 2500 parts of Radix Scutellariaes, 5000 parts of Fructus Forsythiaes | 100 parts of 200 parts of microcrystalline Cellulose of hydroxypropyl methylcellulose |
| A3 | 5000 parts of 2500 parts of Fructus Forsythiaes of 2500 parts of Radix Scutellariaes of Flos Lonicerae | 200 parts of polyvidones |
| A4 | 2500 parts of Flos Loniceraes, 2500 parts of Radix Scutellariaes, 5000 parts of Fructus Forsythiaes | 200 parts of microcrystalline Cellulose |
| A4 | 2500 parts of Flos Loniceraes, 2500 parts of Radix Scutellariaes, 5000 parts of Fructus Forsythiaes | / |
Because it is very clean that feeding environment is difficult to keep, and do not notice that a little slight mastitis is easy to develop into serious mastitis.In large-scale plant, seek the suitable cow ratio of suffering from mastitis and be easier to.
120 of the lactating cows of selecting clinical symptoms significantly to make a definite diagnosis to suffer from mastitis.Choice criteria: milk production of cow descends, breast and milk have significantly unusual, the part swelling of breast infection is rubescent, and breast shows unexpected swelling, heating, hardening, the breast district is responsive, even the performance fervescence, diet subtracts greatly or the phenomenon of useless exhausted, down in spirits.
Test grouping and processing are divided into test group and matched group with 120 ill milch cows, 20 every group at random.
Therapeutic scheme: after getting other water for injection dilution of adding medical grade, at the ill breast local injection of cow.Logotype 3 days was 1 course of treatment.Logotype 6 days was 2 courses of treatment.
Therapeutic effect criterion:
| Mastitis | Milk changes | The variation of giving milk | |
| Recovery from illness | Red, swollen, hot, disease disappears | Perusal is normal | Return to normal level |
| Effectively | Red, swollen, hot, disease obviously goes down | No grumeleuse or floccule in the milk | Positive effect is arranged |
| Invalid | Red, swollen, hot, disease disappears not obvious, even increases the weight of | Perusal is obviously unusual in the milk | Do not recover or minimizing |
Therapeutic effect:
| Therapeutic dose and method | The first course of therapy effect | The second course of therapy effect | |
| A1 | The each 4 grams/cattle that grows up, every day 1 time | 18 produce effect | 1 produces effect |
| A2 | The each 4 grams/cattle that grows up, every day 1 time | 16 produce effect | 2 produce effect |
| A3 | The each 2 grams/cattle that grows up, every day 2 times | 12 produce effect | 3 produce effect |
| A4 | The each 2 grams/cattle that grows up, every day 2 times | 14 produce effect | 1 produces effect |
| A5 | The each 2 grams/cattle that grows up, every day 2 times | 12 produce effect | 2 produce effect |
| A5 | The each 4 grams/cattle that grows up, every day 1 time | 8 produce effect | 3 produce effect |
Be analyzed as follows: the SHUANGHUANLIAN effective ingredient that disposable injection is same, obviously without any the help of adjuvant, the SHUANGHUANLIAN therapeutic effect of A5 group is bad, and this may be that drug level disappears rapidly, influences therapeutic effect.Adding microcrystalline cellulose excipients does not have obvious effect to improving the slow release drug effect, and the combination of hydroxypropyl methylcellulose and sodium carboxymethyl cellulose is obvious to the slow release drug action, and therapeutic effect is best.
Be not understanding and amenable to reason the people because larger animal does not resemble, every day, the double injection administration caused the stress of animal easily.Stress itself also can to a certain degree affect the treatment strongly.
The normal injection agent often needs 2 administrations every day, successive administration 3～5 days.In fact this mode is difficult to carry out, and animal is caused bigger stress.Some course of treatment, also there was similar situation in long stubborn disease (as mycoplasma pneumoniae of swine).Just become the task of top priority so prevent and treat the serious symptom bacterial disease with some stubborn disease, but present alternative durative action preparation is few with the antibacterials durative action preparation.Therefore, the developmental research to long-acting injection is very necessary.The combination of hydroxypropyl methylcellulose of the present invention and sodium carboxymethyl cellulose is kept long-acting drug action contribution obviously to slow release.
Radix Scutellariae of the present invention adopts acid-base method, extracts, makes with extra care the effective ingredient baicalin, and purity reaches more than 90%; Flos Lonicerae, Fructus Forsythiae adopt decoction and alcohol sedimentation technique, extract, make with extra care effective ingredient such as chlorogenic acid, phillyrin.Then with the effective ingredient dosing, the spray-dried sterile injection powder product of making.
The application of ZHUSHEYONG SHUANGHUANGLIAN in pig syndrome of breeding and respiration disorders.
ZHUSHEYONG SHUANGHUANGLIAN treatment mammitis of cow has effect unique.
The invention has the advantages that: the combination of hydroxypropyl methylcellulose and sodium carboxymethyl cellulose is kept long-acting drug action contribution obviously to slow release.And ZHUSHEYONG SHUANGHUANGLIAN adopts the spray drying method molding, and technology is easy, and quick, constant product quality is suitable for the quick production requirement of industrialization.
ZHUSHEYONG SHUANGHUANGLIAN as the herbal medicine compound preparation of the present invention has effects such as antiviral, antibiotic, antiinflammatory, antipyretic-antalgic and raising immunity.Therefore, its veterinary clinic is used for epidemic diarrhea, transmissible gastroenteritis, baby swine paratyphoid, various eqpidemic diseases cause septicemia, diseases such as the cough that respiratory tract disease such as streptococcicosis, pig hemophilus disease, contagious pleuropneumonia, pig lung plague cause, dyspnea, skin cyanosis, arthroncus, limping.Its new application can be used for pig syndrome of breeding and respiration disorders, mammitis of cow.
Chinese medicine injection for animals of the present invention has many advantages with the powder pin,, safety little, efficient, steady quality, easy to carry etc. as effective definite ingredients, content height, toxicity, compare with existing herbal medicine preparation, drug effect is remarkable, the bioavailability height, dosage is little, has really reached quick-acting, efficient, long lasting purposes.Therefore ZHUSHEYONG SHUANGHUANGLIAN of the present invention will be brought into play bigger effect on veterinary clinic.
ZHUSHEYONG SHUANGHUANGLIAN of the present invention is present unique Chinese medicine veterinary drug powder injection formulation of evaluating by the Ministry of Agriculture, and has authorized new veterinary drug certificate of registry.
Specific embodiment
Embodiment 1
As the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, make: 1250 parts of Flos Loniceraes, 3750 parts of Radix Scutellariaes, 2500 parts of Fructus Forsythiaes, 0 part of slow-release auxiliary material by following parts of medicines by weight.
Embodiment 2
As the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, make: 3750 parts of Flos Loniceraes, 1250 parts of Radix Scutellariaes, 7500 parts of Fructus Forsythiaes, 400 parts of slow-release auxiliary material by following parts of medicines by weight.
Embodiment 3
As the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, make: 2500 parts of Flos Loniceraes, 2500 parts of Radix Scutellariaes, 5000 parts of Fructus Forsythiaes, 300 parts of slow-release auxiliary material by following parts of medicines by weight.
Embodiment 4
As the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, slow-release auxiliary material is 200 parts of hydroxypropyl methylcellulose.All the other are with embodiment 3.
Embodiment 5
As the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, slow-release auxiliary material is 200 parts of hydroxypropyl methylcellulose, 100 parts of sodium carboxymethyl cellulose.All the other are with embodiment 3.
Embodiment 6
Preparation technology as the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation:
(1) Radix Scutellariae decocts with water three times, and collecting decoction is transferred pH to 1.0～2.0, leave standstill, filter, precipitation adds the water of 8 times of volumes, transfer pH to 7.0, add the ethanol with water volume equivalent again, stir and make dissolving, filter, filtrate is transferred pH to 2.0, and 60 ℃ are incubated 30 minutes, leave standstill, filter, precipitation is washed pH to 4.0 with ethanol, the water that adds 10 times of volumes stirs, and transfers pH to 6.0, add 0.5% active carbon, fully stir, 50 ℃ are incubated 30 minutes, add 1 times of volume of ethanol and stir, filter, filtrate is transferred pH to 2.0,60 ℃ are incubated 30 minutes, leave standstill, and filter, after precipitation is washed with small amount of ethanol, drying, standby;
(2) Flos Lonicerae, Fructus Forsythiae decoct with water secondary, filter, and it is 1.20～1.25 that filtrate is concentrated into 70～80 ℃ relative density, cooling adds ethanol, makes to contain the alcohol amount and reach 75%, fully stir, leave standstill, the leaching supernatant reclaims ethanol to there not being the alcohol flavor, the water that adds 3～4 times of volumes, transfer pH to 7.0, fully stir and be heated to and boil, leave standstill, the leaching supernatant, the relative density that is concentrated into 70～80 ℃ is 1.10～1.15, and cooling adds ethanol and makes and contain the alcohol amount and reach 85%, leave standstill, the leaching supernatant reclaims ethanol to there not being the alcohol flavor, and is standby;
(3) get Radix Scutellariae extract, it is an amount of to add water, transfers pH to 7.0, heating makes dissolving, adds Flos Lonicerae, Fructus Forsythiae extract, concentrate approximately to 80 ℃ relative density be 1.15, add 0.5% active carbon, keep little the boiling 15 minutes of pH value 7.0 heating, cooling, add slow-release auxiliary material, filter, spray drying is received powder, packing promptly gets injectable powder.All the other are with embodiment 5.
Embodiment 7
Preparation technology as the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, made by following parts of medicines by weight: 2500 parts of Flos Loniceraes, 2500 parts of Radix Scutellariaes, 5000 parts of Fructus Forsythiaes, 300 parts of slow-release auxiliary material, slow-release auxiliary material specifically are 200 parts of hydroxypropyl methylcellulose, 100 parts of sodium carboxymethyl cellulose.
(3) get Radix Scutellariae extract, it is an amount of to add water, transfers pH to 7.0, heating makes dissolving, adds Flos Lonicerae, Fructus Forsythiae extract, concentrate approximately to 80 ℃ relative density be 1.15, add 0.5% active carbon, keep little the boiling 15 minutes of pH value 7.0 heating, cooling, add hydroxypropyl methylcellulose, sodium carboxymethyl cellulose, filter, powder is received in lyophilization, packing promptly gets injectable powder.All the other technologies are with embodiment 6.
Claims (3)
1. as the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, it is characterized in that making: 200 parts of Flos Lonicerae 1250-3750 parts, Radix Scutellariae 1250-3750 part, Fructus Forsythiae 2500-7500 part, slow-release auxiliary material by following parts of medicines by weight; Slow-release auxiliary material is a hydroxypropyl methylcellulose.
2. as the ZHUSHEYONG SHUANGHUANGLIAN of herbal medicine compound preparation, it is characterized in that: 300 parts of Flos Lonicerae 1250-3750 parts, Radix Scutellariae 1250-3750 part, Fructus Forsythiae 2500-7500 part, slow-release auxiliary material; Slow-release auxiliary material is 200 parts of hydroxypropyl methylcellulose, 100 parts of sodium carboxymethyl cellulose.
3. the preparation technology of the described ZHUSHEYONG SHUANGHUANGLIAN as the herbal medicine compound preparation of claim 1 is characterized in that:
(1) Radix Scutellariae decocts with water three times, and collecting decoction is transferred pH to 1.0～2.0, leave standstill, filter, precipitation adds the water of 8 times of volumes, transfer pH to 7.0, add the ethanol with water volume equivalent again, stir and make dissolving, filter, filtrate is transferred pH to 2.0, and 60 ℃ are incubated 30 minutes, leave standstill, filter, precipitation is washed pH to 4.0 with ethanol, the water that adds 10 times of volumes stirs, and transfers pH to 6.0, add 0.5% active carbon, fully stir, 50 ℃ are incubated 30 minutes, add 1 times of volume of ethanol and stir, filter, filtrate is transferred pH to 2.0,60 ℃ are incubated 30 minutes, leave standstill, and filter, after precipitation is washed with small amount of ethanol, drying, standby;
(2) Flos Lonicerae, Fructus Forsythiae decoct with water secondary, filter, and it is 1.20～1.25 that filtrate is concentrated into 70～80 ℃ relative density, cooling adds ethanol, makes to contain the alcohol amount and reach 75%, fully stir, leave standstill, the leaching supernatant reclaims ethanol to there not being the alcohol flavor, the water that adds 3～4 times of volumes, transfer pH to 7.0, fully stir and be heated to and boil, leave standstill, the leaching supernatant, the relative density that is concentrated into 70～80 ℃ is 1.10～1.15, and cooling adds ethanol and makes and contain the alcohol amount and reach 85%, leave standstill, the leaching supernatant reclaims ethanol to there not being the alcohol flavor, and is standby;
(3) get Radix Scutellariae extract, it is an amount of to add water, transfers pH to 7.0, heating makes dissolving, adds Flos Lonicerae, Fructus Forsythiae extract, concentrate approximately to 80 ℃ relative density be 1.15, add 0.5% active carbon, keep little the boiling 15 minutes of pH value 7.0 heating, cooling, add slow-release auxiliary material, filter, spray drying is received powder, packing promptly gets injectable powder.
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| CN103844143B (en) * | 2014-03-11 | 2016-01-27 | 广西大学 | Tilapia mossambica prevention streptococcosis compound Chinese herbal medicinal feed additive |
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