CN101386826B - Engineering strain for water processing - Google Patents
Engineering strain for water processing Download PDFInfo
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- CN101386826B CN101386826B CN2008101368547A CN200810136854A CN101386826B CN 101386826 B CN101386826 B CN 101386826B CN 2008101368547 A CN2008101368547 A CN 2008101368547A CN 200810136854 A CN200810136854 A CN 200810136854A CN 101386826 B CN101386826 B CN 101386826B
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- engineering strain
- water
- zxy30
- water processing
- water treatment
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Abstract
The invention relates to an engineering strain for water treatment, which solves the problem that organic pollutants in water can not be effectively removed by the chemical method in the prior art but residual chemical agent is remained in a treated water body and pollutants with strong carcinogenicity are generated. The engineering strain for water treatment zxy30 is pseudomonas putida, belongs to pseudomonas, and is preserved in the CGMCC, with the preserving date of July 16th, 2008 and a preserving number of CGMCC No. 2600; and the engineering strain for water treatment zxy30 is Gram-negative bacteria, is of a bar shape, and has no spore; and the length of the engineering strain is between 1 and 4 micrometers and the width of the engineering strain is between 0.5 and 1 micrometer,. The engineering strain for water treatment zxy 30 can effectively remove the organic pollutants in the water during the water treatment process, reduce the CODMn of the water body, and metabolize the organic pollutants into harmless inorganic substances such as carbon dioxide, and can reduce the content of a chemical agent for water treatment and the residue amount in the water simultaneously.
Description
Technical field
The present invention relates to a kind of engineering of water treatment bacterial strain.
Background technology
Drinking water source pollutes and increases the weight of the serious safe drinking water that threatens the common people day by day at present.The kind and the quantity of pollutent is being on the increase in the natural water at present; Adopt existing chemical treatment technologies such as " coagulations → deposition → sand filtration → throwing chlorine disinfectant " not only can not effectively remove the organic pollutant in the water, on the contrary residual chemical agent and produced pollutent in the water body after processing also with strong carinogenicity.
Summary of the invention
The objective of the invention is to carry out water treatment and can not effectively remove the organic pollutant in the water in order to solve present employing chemical method; Residual chemical agent and produced the problem of pollutent in the water body after processing also on the contrary, and a kind of engineering strain for water processing that provides with strong carinogenicity.
Engineering strain for water processing zxy30 is a pseudomonas putida, belongs to Rhodopseudomonas; Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preservation date is on July 16th, 2008, and preserving number is CGMCC No.2600; Engineering strain for water processing zxy30 is Gram-negative bacteria, and is shaft-like, and length is 1.5～4 μ m, and wide is 0.5～1 μ m, no gemma; Bacterium colony is a white, translucent, neat in edge, and corrugationless has an even surface.
Engineering strain for water processing zxy30 of the present invention is inoculated in the nutrient broth liquid nutrient medium (the medium pH value is 6.0～8.0) enrichment culture to bacterium colony concentration reaches 5 * 10 under 20～40 ℃ of conditions
9Cfu/mL adopts conventional bio-film colonization technology can prepare the bio-film colonization gac then.Utilize the water treatment capacity of the bio-film colonization gac of engineering strain for water processing zxy30 acquisition of the present invention to significantly improve, under all identical situation of other condition, compare COD with common gac
MnClearance improved more than 15%; And prolonged 0.5～2 times the work-ing life of bio-film colonization gac.Its iodine number is not less than 800mg/L after the bio-film colonization gac that measuring utilizes engineering strain for water processing zxy30 of the present invention to obtain uses 1 year.
Engineering strain for water processing zxy30 of the present invention can effectively remove the organic pollutant in the water, the COD of reduction water body in water treatment procedure
Mn, be harmless inorganicss such as carbonic acid gas with the organic pollutant metabolism; Simultaneously can reduce the consumption of chemistry in water treatment medicament and the residual quantity in the water, significantly reduce the common people's pathogenic risk.
The strong adhesion of engineering strain for water processing zxy30 of the present invention on gac can effectively be fixed on activated carbon surface, and shows through acute toxicological experiment, and it irritates stomach, mouse Non Apparent Abnormality situation to the mouse maximal dose.Therefore engineering strain for water processing zxy30 of the present invention is the microbial strains to the person poultry safety.And engineering strain for water processing zxy30 size of the present invention can guarantee effluent characteristics through the follow-up flocculation sediment technology removal of water treatment more greatly.
Engineering strain for water processing zxy30 is pseudomonas putida (Pseudomonas putida), belongs to Rhodopseudomonas (Pseudomonas); Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), the preservation address is the Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, and preservation date is on July 16th, 2008, preserving number is CGMCC No.2600.
Embodiment
Embodiment one: this embodiment engineering strain for water processing zxy30 is a pseudomonas putida, belongs to Rhodopseudomonas; Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preservation date is on July 16th, 2008, and preserving number is CGMCC No.2600; Engineering strain for water processing zxy30 is Gram-negative bacteria, and is shaft-like, and length is 1.5～4 μ m, and wide is 0.5～1 μ m, no gemma; Bacterium colony is a white, translucent, neat in edge, and corrugationless has an even surface.
This embodiment engineering strain for water processing zxy30 screens in the Songhua River Harbin river and obtains.
This embodiment engineering strain for water processing zxy30 is that 20～40 ℃, pH value are to grow in 6.0～8.0 the environment in temperature.
This embodiment engineering strain for water processing zxy30 has mobility.
This embodiment engineering strain for water processing zxy30 sodium malonate utilization test is positive, mannose ferment is negative, lactose fermentation is negative, Soduxin utilization test is positive, methyl red test is negative, negative, the KNO of rhamnosyl test
3Test positive; The V-P test is negative; Starch test is negative; The NaCl salt tolerance test of mass concentration 2% is positive; The NaCl salt tolerance test of mass concentration 3% is positive; The NaCl salt tolerance test of mass concentration 5% is negative; The NaCl salt tolerance test of mass concentration 7% is negative; The NaCl salt tolerance test of mass concentration 10% is negative; The starch hydrolysis experiment is negative; The N.F,USP MANNITOL test is negative; Gelatin liquification test is positive; The oxysuccinic acid test is negative; The product indole test is positive; Sodium benzoate test is negative; Lipase test is positive; The Sodium.alpha.-hydroxypropionate test is negative; Hydrogen sulfide production test is negative; The sodium succinate test is positive; Catalase test is negative; The product ammonia test is negative; The sodium acetate test is negative; Oxidase test is negative; Glucose test is negative.
This embodiment engineering strain for water processing zxy30 is inoculated in the nutrient broth liquid nutrient medium (the medium pH value is 6.0～8.0) enrichment culture to bacterium colony concentration reaches 5 * 10 under 20～40 ℃ of conditions
9Cfu/mL, adopt then conventional bio-film colonization technology can prepare the bio-film colonization gac (be about to gac and be immersed in the engineering strain zxy30 enrichment culture liquid, through adsorption with engineering strain for water processing zxy30 bio-film colonization on gac).To be fixed with on every gram bio-film colonization gac that engineering strain for water processing zxy30 is about be 6 * 10 through measuring
6Cfu.
This embodiment engineering strain for water processing zxy30 is in the breeding of activated carbon surface; With the activated carbon surface adsorbed organic matter is nutriment, and is translated into carbonic acid gas and living weight, thereby has not only removed the organism in the former water; And make regeneration of activated carbon to a certain extent; Thereby make gac have the ability of lasting adsorb organic compound, promptly prolonged the work-ing life and the regeneration period of gac widely, played biological reinforced effect.The bio-film colonization gac that this embodiment obtains can be used for " ozone-active carbon " technology, not only improves water treatment capacity, and the stability of enhanced system and impact resistance.
Utilize the water treatment capacity of the bio-film colonization gac of this embodiment engineering strain for water processing zxy30 acquisition to significantly improve, under all identical situation of other condition, compare COD with common gac
MnClearance improved 17%, and prolonged 0.5～2 times the work-ing life of bio-film colonization gac.Its iodine number is 930mg/L after the bio-film colonization gac that measuring utilizes engineering strain for water processing zxy30 of the present invention to obtain uses 1 year.
Claims (2)
1. an engineering strain for water processing is characterized in that engineering strain for water processing zxy30 is pseudomonas putida (Pseudomonas putida), belongs to Rhodopseudomonas (Pseudomonas); Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preservation date is on July 16th, 2008, and preserving number is CGMCC No.2600; Engineering strain for water processing zxy30 is Gram-negative bacteria, and is shaft-like, and length is 1.5～4 μ m, and wide is 0.5～1 μ m, no gemma; Bacterium colony is a white, translucent, neat in edge, and corrugationless has an even surface.
2. a kind of engineering strain for water processing according to claim 1 is characterized in that engineering strain for water processing zxy30 is that 20～40 ℃, pH value are to grow in 6.0～8.0 the environment in temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101368547A CN101386826B (en) | 2008-07-31 | 2008-07-31 | Engineering strain for water processing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101368547A CN101386826B (en) | 2008-07-31 | 2008-07-31 | Engineering strain for water processing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101386826A CN101386826A (en) | 2009-03-18 |
| CN101386826B true CN101386826B (en) | 2012-01-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2008101368547A Expired - Fee Related CN101386826B (en) | 2008-07-31 | 2008-07-31 | Engineering strain for water processing |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993837B (en) * | 2010-06-21 | 2012-06-27 | 浙江工业大学 | High-activity high-tolerance formaldehyde degrading bacteria and application thereof |
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2008
- 2008-07-31 CN CN2008101368547A patent/CN101386826B/en not_active Expired - Fee Related
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Granted publication date: 20120111 Termination date: 20120731 |