CN101993837B

CN101993837B - High-activity high-tolerance formaldehyde degrading bacteria and application thereof

Info

Publication number: CN101993837B
Application number: CN2010102045367A
Authority: CN
Inventors: 钟卫鸿; 金晶; 邱乐泉; 吴石金; 钟莉; 徐云; 郑重; 陈建孟
Original assignee: Zhejiang University of Technology ZJUT
Current assignee: Zhejiang University of Technology ZJUT
Priority date: 2010-06-21
Filing date: 2010-06-21
Publication date: 2012-06-27
Anticipated expiration: 2030-06-21
Also published as: CN101993837A

Abstract

The invention provides high-activity high-tolerance formaldehyde degrading bacteria and an application thereof in micro-biological degradation of formaldehyde. In the invention, Pseudomonas putida xyz-zjut is conserved at China Center for Type Culture Collection; the address is Wuhan University, Luojia Mountain, Wuhan City, Hubei Province, 430072; the conservation date is May 23, 2010; and the conservation number is CCTCC No: M 2010125. The invention has the beneficial effects that the high-activity high-tolerance formaldehyde degrading bacteria and the application thereof provide a basis for biological control and treatment of formaldehyde and have wide application prospect.

Description

High tolerance methanal degradation bacteria of high reactivity and application thereof

(1) technical field

The present invention relates to active high tolerance methanal degradation bacteria of a plant height and application thereof.

(2) background technology

In recent years, because a large amount of uses of various art works, coating and caking agent, make that various concentration of narmful substances constantly increase in the room air.Wherein, many materials have stronger toxicity or carinogenicity, and formaldehyde is exactly wherein a kind of.Formaldehyde is a kind of primary toxin, and it is prone to addition, oxidation, reduction, polyreaction take place, and better water solubility is arranged simultaneously.Formaldehyde has teratogenesis and carinogenicity, on the list of the preferential control of China's noxious chemical, occupies the 2nd.Therefore find the formaldehyde in indoor air Countermeasures for Pollution Prevention very urgent, significant to human health.

The biological process control microbial method of especially degrading is one of our important method of seeking.Yet at present domestic report about the Degradation Formaldehyde bacterium seldom.Huang Saihua etc. (2007) report (Huang Saihua; Old ability field. the isolation identification [J] of a strain Degradation Formaldehyde fungi Aspergillus spp.H4. ecotope; 2007; 16 (4): 1175-1179.) screening has obtained 1 strain Degradation Formaldehyde fungi flavus, it can be in 144h with the 1.241g/L Degradation Formaldehyde to 0.004g/L; (Lv Yang such as Lv Yang; Liu Jing, Lv Ping Nan, etc. bio-trickling filter is handled the experimental study [J] of formaldehyde and triphen mixed gas. University Of Tianjin's journal; 2007; 40 (10): 1215-1219.) use to drip filter tower and handle a formaldehyde and a triphen, and therefrom isolated 1 strain Degradation Formaldehyde bacterium pseudomonas (Pseudomonas putida), this bacterium can 72h in the 26.2mg/L Degradation Formaldehyde to 2.5mg/L.External more relatively about the report of Degradation Formaldehyde bacterium.(Iwahara M such as Iwahara; Fukuda R; Nakahara K, et al.Isolation andproperties of Paecilomyces sp.no.5 capable of degrading high concentrationsof formaldehyde [J] .Biocontrol Sci, 2002; 7 (2): 107-110.) separated 1 strain Paecilomyces, the formaldehyde of the 20g/L that can within 20d, degrade fully; (Yamazaki T such as Yamazaki; Tsugawa W; Sode K.Biodegradation of formaldehyde by aformaldehyde-resistant bacterium isolated from seawater [J] .Appl BiochemBiotechnol; 2001,91-3:213-217.) in seawater, separated 1 strain formaldehyde resistant bacteria, it is ability degradation of formaldehyde 0.4g/L in 3% sodium-chlor; (Mirdamadi S such as Mirdamadi; RajabiA; Khalilzadeh P; Et al.Isolation of bacteria able to metabolize highconcentrations of formaldehyde [J] .World Journal of Microbiology &Biotechnology, 2005,21 (6-7): 1299-1301.) report P.pseudoalcaligenes OSS bacterial strain can be in 24h be that 3.7g/L formaldehyde is degraded fully with concentration; 70% concentration of degrading in the 72h is the formaldehyde of 5.92g/L, and Methylobacterium.extorquens ESS and the Methylobacterium.extorquens PSS concentration of degrading fully is the formaldehyde of 2.96g/L; (Adroer N such as Adroer; Casas C, Demas C, et al.Mechanism of Formaldehyde Biodegradation byPseudomonas-Putida [J] .Appl Microbiol Biotechnol; 1990,33 (2): 217-220.) isolating Pseudomonas putida A ₂The bacterial strain concentration of degrading is the formaldehyde of 0.25g/L; (Eiroa M such as Eiroa; Kennes C; Veiga M C.Formaldehyde biodegradation and itsinhibitory effect on nitrification [J] .J Chem Technol Biotechnol; 2004,79 (5): 499-504.) report is to add carbon source with methyl alcohol, also can the degrade formaldehyde of 0.03～3.89g/L of nitrobacteria; Formaldehyde (the Eiroa M of 1.36g/L and denitrifying bacterium also can be degraded at formaldehyde and methyl alcohol and when depositing; Vilar A; Kennes C; Et al.Formaldehyde biodegradation in thepresence of methanol under denitrifying conditions [J] .J Chem TechnolBiotechnol, 2006,81 (3): 312-317.); (Kondo T such as Kondo; Morikawa Y; Hayashi N, et al.Purification and characterization of formate oxidase from aformaldehyde-resistant fungus [J] .Fems Microbiol Lett, 2002; 214 (1): 137-142.) from soil, separated 1 strain formaldehyde tolerance fungi Aspergillus nomiusIRI013, can also grow during for 4.5g/L and fall the formaldehyde completely consumed at concentration of formaldehyde; (Mitsui R such as Mitsui; Omori M; Kitazawa H, et al.Formaldehyde-limited cultivationof a newly isolated methylotrophic bacterium, Methylobacterium sp MF 1:Enzymatic analysis related to C-1metabolism [J] .J Biosci Bioeng; 2005,99 (1): 18-22.) separated the 1 strain methylotrophic bacteria Methylobacterium sp.MF1 1.2g/L formaldehyde of can in 200h, degrading.The Degradation Formaldehyde bacterium of reporting both at home and abroad so far, the tolerance concentration of its formaldehyde and degrading activity are all high not enough, have influenced its practical application.

(3) summary of the invention

The object of the invention provides active high tolerance methanal degradation bacteria of a plant height and the application in microbiological deterioration formaldehyde thereof.

The technical scheme that the present invention adopts is:

Active high tolerance methanal degradation bacteria---pseudomonas putida (Pseudomonasputida) xyz-zjut of one plant height; Be preserved in Chinese typical culture collection center; Address: Luojia Mountain, Wuhan, Hubei Province Wuhan University; 430072, preservation date on May 23rd, 2010, preserving number is CCTCCNo:M 2010125.

The 16S rDNA sequence of this bacterial strain is following:

1 actattgaat?gcattcgagc?ggacgacggg?agcttgctcc?ttgattcagc?ggcgtacggg

61 tgagtaatgc?ctaggaatct?gcctggtagt?gggggacaac?gtttcgaagg?gaacgctaat

121?accgcatacg?tcctacggga?gaaagcaggg?gaccttcggg?ccttgcgcta?tcagatgatc

181?ctaggtctga?ttagctagtt?ggtggggtaa?tggctcacca?aggcgacgat?ccgtaactgg

241?tctgagagga?tgatcagtca?cactggaact?gagacacggt?ccagactcct?acgggaggca

301?gcagtgggga?atattggaca?atgggcgaaa?gcctgatcca?gccatgccgc?gtgtgtgaag

361?aaggtcttcg?gattgtaaag?cactttaagt?tgggaggaag?ggcagtaagt?taataccttg

421?ctgttttgac?gttaccgaca?gaataagcac?cggctaactc?tgtgccagca?gccgcggtaa

481 tacagagggt?gcaagcgtta?atcggaatta?ctgggcgtaa?agcgcgcgta?ggtggtttgt

541 taagttggat?gtgaaagccc?cgggctcaac?ctgggaactg?catccaaaac?tggcaagcta

601 gagtacggta?gagggtggtg?gaatttcctg?tgtagcggtg?aaatgcgtag?atataggaag

661 gaacaccagt?ggcgaaggcg?accacctgga?ctgatactga?cactgaggtg?cgaaagcgtg

721 gggagcaaac?aggattagat?accctggtag?tccacgccgt?aaacgatgtc?aactagccgt

781 tggaatcctt?gagattttag?tggcgcagct?aacgcattaa?gttgaccgcc?tggggagtac

841 ggccgcaagg?ttaaaactca?aatgaattga?cgggggcccg?cacaagcggt?ggagcatgtg

901 gtttaattcg?aagcaacgcg?aagaacctta?ccaggccttg?acatgcagag?aactttccag

961 agatggattg?gtgccttcgg?gaactctgac?acaggtgctg?catggctgtc?gtcagctcgt

1021?gtcgtgagat?gttgggttaa?gtcccgtaac?gagcgcaacc?cttgtcctta?gttaccagca

1081?cgtaatggtg?ggcactctaa?ggagactgcc?ggtgacaaac?cggaggaagg?tggggatgac

1141?gtcaagtcat?catggccctt?acggcctggg?ctacacacgt?gctacaatgg?tcggtacaga

1201?gggttgccaa?gccgcgaggt?ggagctaatc?tcacaaaacc?gatcgtagtc?cggatcgcag

1261?agacaacttg?actgcgtgaa?gtcggaatcg?ctagtaatcg?cgaatcagaa?tgtatcggtg

1321?aatacgttcc?cgggccttgt?acacaccgcc?cgtcacacca?tgggagtggg?tcgcaccaga

1381?agtagttagt?ctaaccttcg?ggacgtcgtt?cagcacggta?gatccagtgc?tcaagcct

The tolerance concentration of this bacterial strain PARA FORMALDEHYDE PRILLS(91,95) can reach 6g/L, and 54h can degrade 86% with it, and 46h can all degrade 5g/L formaldehyde, the 35h 4g/L formaldehyde of can all degrading, the formaldehyde tolerance concentration of this bacterial strain and the Degradation Formaldehyde bacterium height of degradation rate than existing bibliographical information.Said degraded can be carried out in being applicable to the substratum of pseudomonas putida.

The invention still further relates to the application of described pseudomonas putida xyz-zjut in microbiological deterioration formaldehyde.

The best degradation condition of this bacterial strain is 30 ℃, pH8.0.The optimal medium that is applicable to this bacterial strain consists of: peptone 1.2g/L, KH ₂PO ₄4g/L, K ₂HPO ₄3g/L, MgSO ₄7H ₂O 0.2g/L, micro-mother liquor 0.1mL/L, solvent are water; Said micro-mother liquor is formed as follows: H ₃BO ₃6g/L, CoCl ₂6H ₂O 4g/L, ZnSO ₄7H ₂O 2g/L, MnCl ₂4H ₂O 0.6g/L, Na ₂MoO ₄7H ₂O 0.6g/L, NiCl ₂6H ₂O 0.4g/L, CuCl ₂2H ₂O 0.2g/L, solvent are water.

This bacterial strain can be used for the biological treatment of formaldehyde-containing wastewater, also can be used for containing the biopurification of formaldehyde exhaust-gas, for example; Can make biomembrane filled tower, film is inoculated, hung to high-efficiency strain in biomembrane filled tower, adopt counter-current operation; By cat head circulated sprinkling liquid from top to bottom, waste gas then by getting into packing tower at the bottom of the tower, contacts with spray liquid is reverse; In uphill process, contact and be cleaned with the wetting microbial film of filling surface, purified gas is discharged by cat head.

Beneficial effect of the present invention is mainly reflected in: the invention provides active high tolerance methanal degradation bacteria of a plant height and application method thereof, handle formaldehyde for biological control and provide the foundation, have a extensive future.

(4) description of drawings

Fig. 1 is a stereoscan photograph;

Fig. 2 is the BACTERIAL PHYLOGENY evolutionary tree;

Fig. 3 is the influence (2g/L formaldehyde, degradation time 24h, pH7,180r/min, 30 ℃) of different nitrogen sources to degradation by bacteria formaldehyde;

Fig. 4 is the influence (2g/L formaldehyde, degradation time 24h, pH7,180r/min, 30 ℃) of peptone concentration to degradation by bacteria formaldehyde;

Fig. 5 is KH ₂PO ₄Influence (3g/L formaldehyde, degradation time 24h, pH7,180r/min, 30 ℃) to degradation by bacteria formaldehyde;

Fig. 6 is K ₂HPO ₄Influence (3g/L formaldehyde, degradation time 24h, pH7,180r/min, 30 ℃) to degradation by bacteria formaldehyde;

Fig. 7 is MgSO ₄7H ₂O is to the influence (3g/L formaldehyde, degradation time 24h, pH7,180r/min, 30 ℃) of degradation by bacteria formaldehyde;

Fig. 8 is the influence (3g/L formaldehyde, degradation time 24h, pH7,180r/min, 30 ℃) of micro-mother liquor to degradation by bacteria formaldehyde;

Fig. 9 influences figure (3g/L formaldehyde, degradation time 24h, 180r/min, 30 ℃) for the pH value to degradation by bacteria formaldehyde;

Figure 10 be temperature to the influence of degradation by bacteria formaldehyde (3g/L formaldehyde, degradation time 24h, pH7,180r/min);

Figure 11 is a different concns Degradation Formaldehyde process.

(5) embodiment

Below in conjunction with specific embodiment the present invention is described further, but protection scope of the present invention is not limited in this:

Embodiment 1:

1 materials and methods

1.1 material

1.1.1 bacterial classification source

Separating 1 strain that obtains from the discarded agent treated point soil of Zhejiang Polytechnical University can be the bacterial strain of sole carbon source with formaldehyde.

1.1.2 substratum and reagent

Minimum medium is formed: KH ₂PO ₄0.7g/L, K ₂HPO ₄0.85g/L, (NH ₄) ₂SO ₄1.2g/L, MgSO ₄7H ₂O 0.1g/L, CaCl ₂0.01g/L, FeSO ₄7H ₂O 0.001g/L, micro-mother liquor 0.1mL, solvent are water, pH7.0,115 ℃ of sterilization 30min.

The trace element mother liquor is formed: H ₃BO ₃6g/L, CoCl ₂6H ₂O 4g/L, ZnSO ₄7H ₂O 2g/L, MnCl ₂4H ₂O 0.6g/L, Na ₂MoO ₄7H ₂O 0.6g/L, NiCl ₂6H ₂O 0.4g/L, CuCl ₂2H ₂O 0.2g/L, solvent are water.

Methyl ethyl diketone solution: 50g ammonium acetate, 6mL glacial acetic acid and 0.5mL methyl ethyl diketone reagent are dissolved in the 100mL water.This solution is preserved at least in refrigerator and can be stablized one month.

1.2 method

1.2.1 formaldehyde detection method

The formaldehyde detection method is drawn an amount of sample in 25mL tool plug graduated tube with reference to revised GB/T 13197-1991 (the mensuration methyl ethyl diketone spectrophotometry [S] of water quality formaldehyde .), is diluted with water to graticule; Adding 2.5mL methyl ethyl diketone solution shakes up; In 60 ℃ of water-baths 15 minutes, take out the cooling back at wavelength 414nm place, be the reference measurement absorbancy with water; Deduct the measured absorbancy of blank pipe, find the content of formaldehyde the sample then from working curve.

1.2.2 the screening of bacterial strain and purifying

The small amount of soil sample is placed the 100mL SPSS; Leave standstill after adding granulated glass sphere concussion 15min, get the 15ml supernatant and add membrane filtration, filter membrane is taken out place minimum medium then with syringe; Add a certain amount of 0.1g/L formaldehyde then, cultivate in 180r/min, 30 ℃ of shaking tables.

A small amount of nutrient solution is applied to the minimum medium flat board of formaldehyde as sole carbon source, petridish is inverted in 30 ℃ of incubators is cultivated, single bacterium colony of observing on colonial morphology and the picking petridish is further identified.

1.2.3 the evaluation of bacterial strain

Use the transmission electron microscope observing ne ar; With gram staining method with bacterium dyeing and examine under a microscope the thalline color; Carry out the physiological and biochemical index (Shen Ping of bacterium by standard method; Fan Xiurong, Li Guangwu. microbiology experiment [M]. Beijing: Higher Education Publishing House, 1999.116-120.) measure.

The amplification employing forward primer 5 of 16S rDNA sequence '-AGAGTTTGATCCTGGCTCGA-3 '; Reverse primer 5 '-AAGGAGGTGATCCAGCCGCA-3 ', be template with total DNA of bacterium, the PCR response procedures is following: 94 ℃ of sex change 4min; 94 ℃ of 50s; 52 ℃ of 1min, 72 ℃ of 2min, 30 circulations; 72 ℃ of 5min, 4 ℃ of preservations.The order-checking of PCR product is accomplished by Shanghai Ying Jun Bioisystech Co., Ltd.

The base sequence of 16S rDNA is carried out BLAST on ncbi database, select the 16S rDNA complete sequence of 9 representative strain bacteriums, utilize software MEGA4.0 constructing system to grow tree, carry out Phylogenetic Analysis.

1.2.4 culture condition is to the influence and the orthogonal optimization thereof of strains for degrading ability

Choose inorganic nitrogen-sourced KNO respectively ₃, (NH) ₂SO ₄, urea, organic nitrogen source peptone and yeast extract paste, measure the Degradation Formaldehyde rate.On the basis of selecting preferable nitrogenous source, investigate of the influence of nitrogenous source addition again to degradation by bacteria formaldehyde, test with the aseptic substratum that contains 2g/L formaldehyde as contrast.

Investigate KH ₂PO ₄, K ₂HPO ₄, MgSO ₄7H ₂O, CaCl ₂, FeSO ₄7H ₂O and micro-mother liquor are to the influence of degradation by bacteria formaldehyde; Investigate the influence of temperature, because of the volatility of formaldehyde relevant (Wang Jinquan, Luo Jianzhong with temperature to degradation by bacteria formaldehyde; Wen Biyan, etc. indoor formaldehyde releasing rule progress [J]. environment and Sustainable development, 2006; 1:24-26.), so each temperature is all set a contrast; Investigate the influence of pH, test with the aseptic substratum that contains 3g/L formaldehyde as contrast to degradation by bacteria formaldehyde.

On the single factor experiment basis, carry out L by the experimental factor and the level of table 1 ₉(3 ⁴) orthogonal test, to obtain more excellent Degradation Formaldehyde condition.Under the optimal conditions that obtains; Further improve concentration of formaldehyde and improve investigating the degradation capability of bacterium to high-concentration formaldehyde, concentration of formaldehyde gets 4,5 and 6g/L, and each concentration of formaldehyde is provided with contrast respectively; Measure concentration of formaldehyde in substratum and the control group at set intervals, draw the Degradation Formaldehyde curve.

Table 1: orthogonal test factor and level

Level	A (KH ₂PO ₄)/g·L ^-1	B (K ₂HPO ₄)/g·L ^-1	C (Peptone)/g·L ^-1	D (MgSO ₄·7H ₂O)/g·L ^-1
					1	2	2	0.3	0.05
2	3	3	0.6	0.1
					3	4	4	1.2	0.2

2 results and discussion

2.1 the screening of bacterial strain and evaluation

Utilize to add the minimum medium that formaldehyde is sole carbon source, from soil, screened the bacterial strain xyz-zjut that 1 strain can utilize formaldehyde, separate obtaining single bacterium colony through dull and stereotyped coating, bacterium colony is rounded, and neat in edge, convexity, color be white, smooth surface.Through transmission electron microscope observing, bacterial strain xyz-zjut is rod-short, and end is given birth to flagellum, and the flagellum number is 2 (Fig. 1).The bacterium gram staining method shows that this bacterial strain is a Gram-negative bacteria.Multinomial physiological and biochemical index to bacterium is measured, and its result is as shown in table 2.Its result and pseudomonas putida (Pseudomonas putida) physiological and biochemical index matees basically, and it is pseudomonas putida for a preliminary evaluation.

Bacterial 16 S rDNA sequence order-checking back is in the GenBank registration, and its accession number is GQ502786.1.Carry out the BLAST comparison through ncbi database; The bacterial strain of display separation belongs to Rhodopseudomonas as a result; Further through comparing with the 16S rDNA complete sequence of other 9 representative strain bacteriums; Make up the systematic evolution tree (Fig. 2) of this bacterial strain, the result shows that separating obtained Degradation Formaldehyde bacterium is for to have 100% similarity with pseudomonas putida.

Table 2: bacterium physiological and biochemical index

2.3 culture condition is to the influence of strains for degrading ability

Different nitrogen sources is to influence such as Fig. 3 of degradation by bacteria formaldehyde.Can find out that by Fig. 3 organic nitrogen source compares the inorganic nitrogen-sourced degradation by bacteria formaldehyde that more helps, reason possibly be bacterium fast breeding under the condition that organic nitrogen source exists, and a large amount of then bacteriums is degradation of formaldehyde simultaneously, can significantly improve Degradation Formaldehyde efficient.As organic nitrogen source, the Degradation Formaldehyde effect that adds peptone is better slightly than yeast extract paste.The different concns peptone is to influence such as Fig. 4 of degradation by bacteria formaldehyde; The result shows that the efficient of degradation by bacteria formaldehyde raises along with the rising of peptone concentration; But Degradation Formaldehyde rate increase rate is very little after having arrived finite concentration, even also reduces to some extent, and reason possibly be that bacterium preferentially utilizes organic nitrogen source; An amount of peptone can promote a large amount of breedings of bacterium at short notice and don't influence the degraded of formaldehyde; If the amount of organic nitrogen source is more, thereby bacterium utilizes organic nitrogen source to influence the utilization of formaldehyde in a large number, causes the degradation efficiency of formaldehyde to can not get significantly improving.

Inorganic salt are the indispensable materials of microbial life activity.Its major function be constitute the thalline composition, as the activator of the moity of enzyme, enzyme or suppressor factor etc.Test has investigated that inorganic salt are to the influence of degradation by bacteria formaldehyde in the substratum, and the efficient of degradation by bacteria formaldehyde is along with KH ₂PO ₄(Fig. 5) and K ₂HPO ₄(Fig. 6) rising of concentration and raising, after reaching certain value, the Degradation Formaldehyde rate can reduce on the contrary.MgSO ₄7H ₂O also can promote degradation by bacteria formaldehyde, works as MgSO ₄7H ₂O (Fig. 7) concentration reaches after the certain value, and the degradation efficiency of formaldehyde can't be because of MgSO ₄7H ₂The rising of O concentration and raising, but remain on a more more stable scope, supposition possibly be because Mg ²⁺It is the cofactor of certain key enzyme in the Degradation Formaldehyde approach.Test-results shows CaCl ₂And FeSO ₄7H ₂O can not promote degradation by bacteria formaldehyde, along with the rising Degradation Formaldehyde rate of these 2 kinds of material concentrations does not have considerable change, so, no longer add this two kinds of materials in the following test.Contain multiple metals ion in the trace element mother liquor; Wherein certain ion possibly be the cofactor of Degradation Formaldehyde key enzyme; The result shows (Fig. 8); Add a spot of micro-mother liquor and can improve Degradation Formaldehyde efficient, too much trace element can make the Degradation Formaldehyde rate reduce on the contrary, possibly be because heavy metal ion to due to the toxic action of bacterium.

Investigate the degradation efficiency of formaldehyde under different pH values and the temperature, its result such as Fig. 9 and shown in Figure 10.Can know that by figure the optimum pH of degradation by bacteria formaldehyde is 8, optimum temperuture is 30 ℃.

Comprehensive above single factor experiment result selects KH ₂PO ₄, K ₂HPO ₄, peptone and MgSO ₄7H ₂O carries out L ₉(3 ⁴) orthogonal test, to obtain more excellent Degradation Formaldehyde condition, its result is as shown in table 3.

Table 3: orthogonal experiments

Can be known that by table 3 primary and secondary of each factor is D＞A, B＞C in proper order, optimum combination is A ₃B ₂C ₃D ₃, both the more excellent condition of degradation by bacteria formaldehyde was: KH ₂PO ₄4g/L, K ₂HPO ₄3g/L, peptone 1.2g/L, MgSO ₄7H ₂O 0.2g/L.

Bacterium degradation process such as Figure 11 under higher concentration of formaldehyde, degradation condition is 180r/min, 30 ℃.Can know by Figure 11, in the contrast formaldehyde volatilization seldom, the tolerance concentration of bacterium PARA FORMALDEHYDE PRILLS(91,95) can reach 6g/L, 54h can degrade 86% with it, 46h can all degrade 5g/L formaldehyde, the 35h 4g/L formaldehyde of can all degrading.Through after optimizing, the tolerance concentration and the degradation efficiency of bacterial strain PARA FORMALDEHYDE PRILLS(91,95) improve greatly, with (Wang Jinquan such as Iwahara; Luo Jianzhong, Wen Biyan, etc. indoor formaldehyde releasing rule progress [J]. environment and Sustainable development; 2006,1:24-26.) isolating Paecilomyces.sp.no.5 compares, and its tolerance concentration (6g/L) is lower than Paecilomyces.sp.no.5 (20g/L); But its degradation efficiency can reach 95mg/Lh, and is higher than the degradation rate of Paecilomyces.sp.no.5 bacterial strain 41.6mg/Lh.

Claims

1. active high tolerance methanal degradation bacteria---pseudomonas putida (Pseudomonas putida) xyz-zjut of a plant height; Be preserved in Chinese typical culture collection center; Address: Luojia Mountain, Wuhan, Hubei Province Wuhan University; 430072, preservation date on May 23rd, 2010, preserving number is CCTCCNo:M 2010125.

2. the application of pseudomonas putida xyz-zjut as claimed in claim 1 in microbiological deterioration formaldehyde.

3. application as claimed in claim 2 is characterized in that said degraded carries out under 30 ℃, pH8.0 condition.

4. application as claimed in claim 3 is characterized in that said degraded carries out: peptone 1.2g/L, KH in forming following substratum ₂PO ₄4g/L, K ₂HPO ₄3g/L, MgSO ₄7H ₂O 0.2g/L, micro-mother liquor 0.1mL/L, solvent are water; Said micro-mother liquor is formed as follows: H ₃BO ₃6g/L, CoCl ₂6H ₂O 4g/L, ZnSO ₄7H ₂O 2g/L, MnCl ₂4H ₂O 0.6g/L, Na ₂MoO ₄7H ₂O 0.6g/L, NiCl ₂6H ₂O 0.4g/L, CuCl ₂2H ₂O 0.2g/L, solvent are water.