CN101384706B - 骨骼肌周成血管细胞和心脏中成血管细胞、其分离方法和用途 - Google Patents
骨骼肌周成血管细胞和心脏中成血管细胞、其分离方法和用途 Download PDFInfo
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- CN101384706B CN101384706B CN2007800056575A CN200780005657A CN101384706B CN 101384706 B CN101384706 B CN 101384706B CN 2007800056575 A CN2007800056575 A CN 2007800056575A CN 200780005657 A CN200780005657 A CN 200780005657A CN 101384706 B CN101384706 B CN 101384706B
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- cell
- angioblast
- muscle
- muscular dystrophy
- skeletal muscle
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
- A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1329—Cardiomyocytes
Abstract
Description
CD45 | CD34 | CD44 | CD31 | Sca-1 | c-kit | |
主动脉E8 | - | + | + | + | + | + |
心室J2 | - | + | + | + | + | + |
主动脉ND | 主动脉DIF | 心室ND | 心室DIF | |
Is1-1 | + | + | + | + |
Nkx2.5 | + | + | + | + |
Gata4 | + | + | + | + |
Gata6 | + | + | + | + |
Mef2a | - | - | - | - |
Mef2c | - | + | - | + |
Tbx2 | + | + | + | + |
Tbx5 | + | + | + | + |
Tbx6 | - | + | - | + |
细胞克隆 | 主动脉E8 | 心室J2 | 心房K1 | 游离壁B8 | 间隔G3 |
自发心肌分化 | >90% | >90% | <1% | <1% | <1% |
共培养诱导的心肌分化 | ~90% | ~80% | ~70% | ~20% | ~10% |
CD31 | CD34 | CD44 | CD45 | CD117 | CD133 | |
心室 | + | + | + | - | + | - |
主动脉 | + | + | + | - | + | - |
心房 | + | + | + | - | + | - |
Nkx2.5 | Is1-1 | Gata4 | Mef2A | Tbx2 | Tbx5 | |
心室 | + | - | + | + | + | + |
主动脉 | + | - | + | + | + | + |
心房 | + | - | + | + | + | + |
% | 5-aza | 共培养 | 自发 |
心室 | 55 | 35 | 无 |
主动脉 | 35 | 19 | 无 |
心房 | 21 | 12 | 无 |
标记 | CPC | ACSC | 成心细胞 | 小鼠心脏 |
- | Schneider | Anversa | Chien | Cossu |
CD45 | - | - | - | - |
CD31 | - | - | ? | + |
CD34 | - | - | - | + |
Sca-1 | + | + | + | + |
c-kit | - | + | + | + |
GATA4 | + | + | + | + |
Nkx2.5 | - | + | ? | + |
NEF2C | + | + | ? | - |
Is1-1 | ? | ? | + | + |
Claims (16)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US77488206P | 2006-02-16 | 2006-02-16 | |
US60/774,882 | 2006-02-16 | ||
PCT/EP2007/001309 WO2007093412A2 (en) | 2006-02-16 | 2007-02-15 | Skeletal muscle periangioblasts and cardiac mesoangioblasts, method for isolation and uses thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101384706A CN101384706A (zh) | 2009-03-11 |
CN101384706B true CN101384706B (zh) | 2013-11-06 |
Family
ID=37930379
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2007800056575A Expired - Fee Related CN101384706B (zh) | 2006-02-16 | 2007-02-15 | 骨骼肌周成血管细胞和心脏中成血管细胞、其分离方法和用途 |
Country Status (6)
Country | Link |
---|---|
US (1) | US8071380B2 (zh) |
EP (1) | EP1991664B1 (zh) |
JP (1) | JP2009531021A (zh) |
CN (1) | CN101384706B (zh) |
CA (1) | CA2642381A1 (zh) |
WO (1) | WO2007093412A2 (zh) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2269461B1 (en) | 2004-07-30 | 2017-03-22 | Mayo Foundation For Medical Education And Research | Treating cardiovascular tissue |
US9765298B2 (en) | 2006-07-24 | 2017-09-19 | Mayo Foundation For Medical Education And Research | Methods and materials for providing cardiac cells |
WO2009145761A1 (en) * | 2008-05-27 | 2009-12-03 | Mayo Foundation For Medical Education And Research | Methods and materials for using cells to treat heart tissue |
US20120100533A1 (en) * | 2009-05-20 | 2012-04-26 | Cardio3 Biosciences Sa | Method for determining the cardio-generative potential of mammalian cells |
BRPI1010684A2 (pt) * | 2009-05-20 | 2017-07-18 | Cardio3 Biosciences Sa | método para determinar o potencial cardiorregenerativo de células mamíferas |
US20110014161A1 (en) * | 2009-07-09 | 2011-01-20 | Xiaozhen Wang | Cardiac Tissue-Derived Cells |
WO2012024573A2 (en) | 2010-08-19 | 2012-02-23 | The Regents Of The University Of California | Compositions comrpising perivascular stem cells and nell-1 protein |
CN104136034B (zh) * | 2011-11-30 | 2018-04-24 | 安斯泰来再生医药协会 | 间充质基质细胞及其相关用途 |
US20140363405A1 (en) | 2012-01-19 | 2014-12-11 | Ucl Business Plc | Method for obtaining mab-like cells and uses thereof |
US20150337266A1 (en) * | 2012-12-28 | 2015-11-26 | Kyoto University | Method for producing induced pluripotent stem cells, cardiomyocytes or precursor cells thereof |
GB201408712D0 (en) * | 2014-05-16 | 2014-07-02 | Univ Leuven Kath | Mesodermal progenitor cells |
JP7029173B2 (ja) * | 2016-03-15 | 2022-03-03 | 国立大学法人 筑波大学 | 多能性幹細胞からの心臓前駆細胞と心筋細胞の製造方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003095631A1 (en) * | 2002-05-13 | 2003-11-20 | Fondazione Centro San Raffaele Del Monte Tabor | Method for establishing and expanding multipotent stem cells |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK1007631T4 (da) | 1997-07-14 | 2009-04-27 | Osiris Therapeutics Inc | Hjertemuskelregeneration ved anvendelse af mesenkymale stamceller |
EP1064356A2 (en) | 1998-03-23 | 2001-01-03 | ZymoGenetics, Inc. | Cardiac-derived stem cells |
US7015037B1 (en) * | 1999-08-05 | 2006-03-21 | Regents Of The University Of Minnesota | Multiponent adult stem cells and methods for isolation |
WO2001094420A1 (en) | 2000-06-05 | 2001-12-13 | The Trustees Of Columbia University In The City Of New York | Identification and use of human bone marrow-derived endothelial progenitor cells to improve myocardial function after ischemic injury |
US7862810B2 (en) * | 2000-07-31 | 2011-01-04 | New York Medical College | Methods and compositions for the repair and/or regeneration of damaged myocardium |
ATE400656T1 (de) * | 2000-10-06 | 2008-07-15 | Univ Michigan | Mini-dystrophin nukleinsäure- und peptidsequenzen |
ITTO20010517A1 (it) | 2001-05-31 | 2002-12-01 | Giulio Alessandri | Cellule progenitrici vascolari umane. |
JP2004535199A (ja) | 2001-07-12 | 2004-11-25 | ジェロン コーポレイション | ヒト多能性幹細胞から産生される心筋細胞系譜の細胞 |
CA2465672A1 (en) | 2001-09-19 | 2003-03-27 | Henry Ford Health System | Cardiac transplantation of stem cells for the treatment of heart failure |
WO2004019767A2 (en) | 2002-08-29 | 2004-03-11 | Baylor College Of Medicine | Heart derived cells for cardiac repair |
ITRM20030376A1 (it) | 2003-07-31 | 2005-02-01 | Univ Roma | Procedimento per l'isolamento e l'espansione di cellule staminali cardiache da biopsia. |
CA2475174A1 (en) | 2004-07-19 | 2006-01-19 | Institut Pasteur | Isolated muscle satellite cells, use thereof in muscle tissue repair and method for isolating said muscle satellite cells |
US7811817B2 (en) * | 2005-05-17 | 2010-10-12 | Reliance Life Sciences Pvt. Ltd. | Establishment of a human embryonic stem cell line using mammalian cells |
-
2007
- 2007-02-15 US US12/279,716 patent/US8071380B2/en active Active
- 2007-02-15 WO PCT/EP2007/001309 patent/WO2007093412A2/en active Application Filing
- 2007-02-15 EP EP07711541.8A patent/EP1991664B1/en active Active
- 2007-02-15 JP JP2008554676A patent/JP2009531021A/ja active Pending
- 2007-02-15 CN CN2007800056575A patent/CN101384706B/zh not_active Expired - Fee Related
- 2007-02-15 CA CA002642381A patent/CA2642381A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003095631A1 (en) * | 2002-05-13 | 2003-11-20 | Fondazione Centro San Raffaele Del Monte Tabor | Method for establishing and expanding multipotent stem cells |
Non-Patent Citations (3)
Title |
---|
Morayma Reyes等.Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells.《blood》.2001,第98卷(第9期),2615-2625. * |
YIN A H等.AC133, a novel marker for human hematopoietic stem and progenitor cells.《blood》.1997,第90卷(第12期),5002-5012. * |
YINAH等.AC133 a novel marker for human hematopoietic stem and progenitor cells.《blood》.1997 |
Also Published As
Publication number | Publication date |
---|---|
US20090317909A1 (en) | 2009-12-24 |
WO2007093412A2 (en) | 2007-08-23 |
EP1991664A2 (en) | 2008-11-19 |
CN101384706A (zh) | 2009-03-11 |
US8071380B2 (en) | 2011-12-06 |
WO2007093412A8 (en) | 2008-11-20 |
EP1991664B1 (en) | 2018-03-28 |
CA2642381A1 (en) | 2007-08-23 |
WO2007093412A3 (en) | 2007-11-22 |
JP2009531021A (ja) | 2009-09-03 |
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