CN101372666B - Composite microbial preparation for methane tank and preparation thereof - Google Patents
Composite microbial preparation for methane tank and preparation thereof Download PDFInfo
- Publication number
- CN101372666B CN101372666B CN2008101404007A CN200810140400A CN101372666B CN 101372666 B CN101372666 B CN 101372666B CN 2008101404007 A CN2008101404007 A CN 2008101404007A CN 200810140400 A CN200810140400 A CN 200810140400A CN 101372666 B CN101372666 B CN 101372666B
- Authority
- CN
- China
- Prior art keywords
- liquid
- bacillus
- kinds
- culture
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Treatment Of Sludge (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to a composite microbial inoculum used in a biogas generating pit, which consists of four kinds of microbial fermentation liquor, namely, 0.06-0.15 part of pseudomonas fluorescens solution, 0.04-0.08 part of waxen bacillus solution, 0.06-0.10 part of bacillus subtillis solution and 0.05-0.08 part of bacillus circulans solution, which are mixed in proportion. The composite microbial inoculum can promote the biogas generating pit to generate biogas normally in winter and can prepare the biogas by taking straws as main raw material.
Description
Technical field
The invention belongs to agriculture microbial technology field, relate in particular to a kind of domestic methane tank aerogenesis and can improve with the stalk complex micro organism fungicide of the methane tank gas production amount that is raw material under cold condition that is used to impel.
Background technology
Along with China's rapid economic development, energy demand constantly increases, and energy shortage is also more and more serious, and the energy of great number impels peasant's pressure to increase.Therefore, making full use of the rural area existing resource is to solve rural energy problem, one of effective measure of reducing farmer's burdens at rural area popularization domestic methane tank.8-10m according to measuring and calculating a bite one average annual normal gas producing
3Methane-generating pit, the annual about 350-400m of biogas that produces
3, about 800kg of alternative coal or the about 1000kg of fuel wood.Can greatly alleviate peasant's economical load.Simultaneously, build domestic methane tank in the rural area and can also eliminate the rural area fecal pollution, reduce ight soil pathogen transmissions such as parasite, the environmental pollution of avoiding straw burning to cause.Greatly purify rural area underground water, thereby improved the rural ecological environment.
But the development of northern China domestic methane tank is subjected to the restriction of two aspects at present.The firstth, the problem of methane-generating pit aerogenesis in winter difficulty.The secondth, be the problem of main raw aerogenesis difficulty with the stalk.The low temperature of north of china in winter has seriously limited the application of methane-generating pit.Content of cellulose height in the stalk, light specific gravity crusts easily and stops up, problem such as input and output material difficulty in addition, the stalk marsh gas fermentation is an a great problem.Therefore quickening the digest and decompose of stalk in methane-generating pit from the angle of adding microbiobacterial agent is the key of dealing with problems.
This patent passes through the organic assembling to the microorganism strains of 4 kinds of excellent performances, and the complex micro organism fungicide of making possesses stronger fecundity under lower temperature, and all kinds of robust fibre and protein macromole are had very strong digestion ability.This complex micro organism fungicide adds the enrichment that can quicken to produce the biogas bacterium in the methane-generating pit to, starts thereby quicken methane-generating pit.Cooperate simple and easy effective insulation measure can make the methane-generating pit normal gas producing in the winter time, and can to increase with the stalk be the gas production rate of the methane-generating pit of main raw material(s).
The methane-generating pit of promoting mainly is main raw material with animal excrement at present, and there are a large amount of idle stalk resources in the rural area, and annual nearly 1.4 hundred million tons of quilts burn.The use of this microbial inoculum will impel a large amount of stalks to obtain transforming in the methane-generating pit kind, and the natural pond slag that forms contains a large amount of trace element and organic, can be used as a kind of fine fertilizer.
Chinese patent file CN1888073 (200510077792.3) discloses a kind of biogas fermentation method of composite fungus agent pretreated straw, composite fungus agent is high temperature Zymomonas mobilis, subtilis, Trichoderma and Bacillus licheniformis, stalk after wetting adds composite fungus agent and as the nitrogenous source bicarbonate of ammonia of stalk stack retting and add water, cover stack retting with plastic cloth, then that stack retting is good stalk is gone into methane-generating pit, add ammonium hydrogencarbonate simultaneously and produce the methane inoculum, airtight 2～7 days, promptly produce biogas.Pretreated stalk fermentation speed and aerogenesis speed are fast, and the initial aerogenesis time is shortened, and factor of created gase improves about 30%, can make the raw material of agricultural crop straws such as all corn stalks, straw, straw as biogas fermentation, and the scope of application is extensive.The limitation of this method is 1, lay particular emphasis on decomposing straw can not solve user's methane-generating pit and produce the biogas problem winter, 2, this composite fungus agent can only use when pretreated straw, can not directly drop into short biogas generation in the methane-generating pit, the using method trouble has limited its popularization.
Summary of the invention
At the deficiencies in the prior art, the invention provides a kind of effectively compound microbial inoculant and preparation method thereof, complex micro organism fungicide of the present invention can directly be added to methane-generating pit, make methane-generating pit normal gas producing in the winter time, and gas production rate does not more increase more than the 30-50% with microbial inoculum, this complex micro organism fungicide can promote with the stalk be main raw material methane-generating pit gas production rate and accelerate aerogenesis speed.
Technical scheme of the present invention is as follows:
A kind of low temperature promotes the complex micro organism fungicide of methane tank gas production down, be by following Pseudomonas fluorescens (Pseudomonasfluorescens), wax bacillus (Bacillus cereus), Bacillus subtillis (Bacillus subtilis) and bacillus circulans (Bacillus circulans) fermented liquid of totally 4 kinds of bacterium be mixed and made into, wherein as follows by weight:
Pseudomonas fluorescens liquid 0.06-0.15 part, wax bacillus liquid 0.04-0.08 part, Bacillus subtillis liquid 0.06-0.10 part, bacillus circulans liquid 0.05-0.08 part.
More than in the fermented liquids of 4 kinds of bacterium living bacteria count respectively all more than 1,000,000,000/milliliter.
The preparation method of complex micro organism fungicide of the present invention, step is as follows:
Cultivate activation, shake-flask culture, seeding tank liquid fermentation and culture, the production of fermentor tank liquid fermenting by slant strains respectively 1.4 plant the fermented liquid of bacterium, working condition is as follows:
(1) wax bacillus liquid:
Spawn culture activation and fermention medium all adopt extractum carnis 5 grams, peptone 5 grams, sodium-chlor 5 grams, yeast powder 1 gram, KH
2PO
40.5 gram, MgSO
40.5 1000 milliliters in gram, tap water, pH value 7.0-7.5;
Fermentation culture conditions: cultivating (fermentation) temperature is 25-28 ℃, and rotating speed is 200 rev/mins, and air flow is 1:1 (v:v/min is with the volume ratio of fermented liquid), is cultured to logarithmic phase.
(2) Pseudomonas fluorescens liquid:
Adopt the LB substratum as spawn culture activatory substratum.Liquid nutrient medium (fermention medium) is formed: glucose 30 grams, extractum carnis 10 grams, NaNO
33 grams, MgSO
47H
2O0.3 gram, KCl0.3 gram, K
2HPO
41.5 1000 milliliters in gram, tap water, pH=6.0-6.5.
Fermentation culture conditions: cultivating (fermentation) temperature is 23-25 ℃, and rotating speed is 160 rev/mins, and air flow is 1:1 (v:v/min is with the volume ratio of fermented liquid), is cultured to logarithmic phase.
(3) Bacillus subtillis liquid:
Adopt the LB substratum as spawn culture activatory substratum.Liquid nutrient medium (fermention medium) consists of: 1000 milliliters in peptone 8g, yeast extract paste 5g, glucose 1g, extractum carnis 3g, tap water, pH7.0.
Fermentation culture conditions: cultivating (fermentation) temperature is 23-25 ℃, and rotating speed is 160 rev/mins, and air flow is 1:1 (v:v/min is with the volume ratio of fermented liquid), is cultured to logarithmic phase.
(4) bacillus circulans liquid:
Adopt the LB substratum as spawn culture activatory substratum.Liquid nutrient medium (fermention medium) consists of: 1000 milliliters in peptone 5g, yeast extract paste 5g, glucose 10g, extractum carnis 2g, tap water, pH7.0.
The bacillus circulans fermentation culture conditions: cultivating (fermentation) temperature is 24-28 ℃, and rotating speed is 160 rev/mins, and air flow is 1:1 (v:v/min is with the volume ratio of fermented liquid), is cultured to logarithmic phase.
2. composite
The fermented liquid of 4 kinds of bacterium of above preparation is mixed in proportion composite making, perhaps adopts adsorbents adsorb to become the solid fungicide formulation mixed liquid bacterial agent.
Described sorbent material is selected the peat composed of rotten mosses for use, one of diatomite or mixing, and the total consumption of sorbent material is (3～10) with the ratio of 4 kinds of bacterium liquid gross weights: 1
What clearly do not limit among the preparation method of above-mentioned complex micro organism fungicide all can be by prior art.
In the complex micro organism fungicide of the present invention, each quasi-microorganism can be in double oxygen, aerobic environment growth.
The suitableeest use temperature of complex micro organism fungicide of the present invention be the methane-generating pit feed temperature more than 9 ℃, the methane-generating pit raw material is to be mixed into main raw material with ight soil, stalk or both.
The present invention compared with prior art has following advantage:
1, the preparation technique of composite fungus agent of the present invention has good repeatability.
2, this complex micro organism fungicide can obviously improve biogas output at low temperatures.As long as feed temperature remains on more than 9 ℃ in the biogas fermentation device, the methane-generating pit that with the stalk is main raw material is the methane-generating pit of main raw material with ight soil, than not more than the raising 40-60% with microbial inoculum than not improving about 30-50% with the microbial inoculum gas production rate.
3, this complex micro organism fungicide can make start time than not shortening 5-12 days with microbial inoculum.
4, the using method of this complex micro organism fungicide is simple, and this microbial inoculum has long-lasting.Because methane-generating pit can use throughout the year, the coal consumption of living that each household on average can be saved three to four months every year.
5, the natural pond slag measurement result that adds behind this complex micro organism fungicide is shown that nitrogen, phosphorus, potassium and organic content are a kind of good organic fertilizer all than higher in the slag of natural pond.
Embodiment
Below in conjunction with embodiment invention is further specified, but the present invention is not only limited to this.If no special instructions, the amount of raw material is a weight part.Buy on the peat composed of rotten mosses, the diatomite market among each embodiment and get final product, should to pulverize the back inadequately standby if buy material fineness.Wherein the peat composed of rotten mosses was 60 mesh sieves, and water-content is the common peat composed of rotten mosses below 10%; Diatomite is crossed 80 mesh sieves, and water-content is common diatomite below 10%.
Embodiment 1:
Pseudomonas fluorescens, the wax bacillus, Bacillus subtillis and bacillus circulans are buied by DSMZ, adopt ordinary method to Pseudomonas fluorescens, the wax bacillus, it is standby that the independent fermentation of Bacillus subtillis and bacillus circulans makes 4 kinds of bacterium liquid, and each fermenation raw liquid living bacteria count should remain on 1,000,000,000/milliliter.Concrete grammar is as follows:
(1) above-mentioned 4 kinds of microorganisms is activated to separately preservation slant medium, cultivated 24-36 hour for 28-30 ℃.
(2) will activate good bacterial classification and receive separately liquid nutrient medium, wax bacillus (Bacillus cereus) is adopted: extractum carnis 5 grams, peptone 5 grams, sodium-chlor 5 grams, yeast powder 1 gram, KH
2PO
40.5 gram, MgSO
40.5 gram, 1000 milliliters in tap water, pH value 7.0-7.5.Pseudomonas fluorescens (Pseudomonas Putida) is adopted: glucose 30 grams, extractum carnis 10 grams, NaNO
33 grams, MgSO
47H
2O0.3 gram, KCl0.3 gram, K
2HPO
41.5 1000 milliliters in gram, tap water, pH=6.0-6.5.Subtilis (Bacillus subtilis) is adopted: 1000 milliliters in peptone 8g, yeast extract paste 5g, glucose 1g, extractum carnis 3g, tap water, pH7.0.23-28 ℃ of following shaking table 160 rev/mins of cultured continuously 24-36 hours gets shake-flask culture liquid, prepares the inoculation seeding tank.
(3) seeding tank is 100 liters, and 70 liters of charging capacitys feed intake according to above-mentioned 4 kinds of microbial liquid culture medium prescriptions respectively, the back 121 ℃ of high pressure moist heat sterilizations that feed intake, and the cooling back is inserted above-mentioned shake-flask culture liquid respectively according to 10% inoculum size.Be cultured to logarithmic phase respectively.
(4) produce 1000 liters of fermentor tanks, feed 700 liters, the substratum of producing jar is with the seeding tank substratum.The back 121 ℃ of high pressure moist heat sterilizations that feed intake, the cooling back is inserted the above-mentioned seed liquor that reaches logarithmic phase respectively according to 10% inoculum size.It is good with 36hr that waxy Bacillus is cultivated (fermentation) time, and cultivating (fermentation) temperature is 26 ℃, and rotating speed is 180 rev/mins, and air flow is 1:1 (with the volume ratio of fermented liquid).It is good with 24hr that Pseudomonas fluorescens is cultivated (fermentation) time, and cultivating (fermentation) temperature is 25 ℃, and rotating speed is 160 rev/mins, and air flow is 1:1.It is good with 28hr that Bacillus circulans is cultivated (fermentation) time, and cultivating (fermentation) temperature is 28 ℃, and rotating speed is 160 rev/mins, and air flow is 1:1.It is good with 28hr that subtilis is cultivated (fermentation) time, and cultivating (fermentation) temperature is 25 ℃, and rotating speed is 160 rev/mins, and air flow is 1:1.
4 kinds of bacterium liquid that will make by above method are by Pseudomonas fluorescens liquid 0.06-0.15 part, wax bacillus liquid 0.04-0.08 part, Bacillus subtillis liquid 0.06-0.10 part, bacillus circulans liquid 0.05-0.08 part is mixed composite, complex micro organism fungicide.
Preparation of fermentation liquid such as the embodiment 1 of embodiment 2:4 kind bacterium.
2 parts of the peats composed of rotten mosses, 1 part in diatomite, the peat composed of rotten mosses and the diatomite back of sterilizing is added mixing tank, mix the back and add microbiobacterial agent, fermentation of bacillus subtilis stoste is 0.1 part in the microbial inoculum, 0.08 part of wax bacillus fermentation stoste, 0.1 part of Pseudomonas fluorescens fermenation raw liquid, 0.05 part of bacillus circulans fermenation raw liquid, the living bacteria count of various bacterium liquid are respectively at 1,000,000,000/milliliter, and it is standby that the microbiobacterial agent and the peat composed of rotten mosses and diatomite mix the back.This composite fungus agent is fit to stalk and ight soil is the methane-generating pit use of main raw material.
Preparation of fermentation liquid such as the embodiment 1 of embodiment 3:4 kind bacterium.
1 part of the peat composed of rotten mosses, 1 part in diatomite, the peat composed of rotten mosses and the diatomite back of sterilizing is added mixing tank and mixes the back and add microbiobacterial agent, fermentation of bacillus subtilis stoste is 0.08 part in the microbial inoculum, 0.04 part of wax bacillus fermentation stoste, 0.15 part of Pseudomonas fluorescens fermenation raw liquid, 0.06 part of bacillus circulans fermenation raw liquid, the living bacteria count of various bacterium liquid is respectively at 1,000,000,000/milliliter, and it is standby that the microbiobacterial agent and the peat composed of rotten mosses and diatomite mix the back.This composite fungus agent is fit to the methane-generating pit use that stalk is a main raw material.
Claims (3)
1. complex micro organism fungicide, it is characterized in that forming by the fermented liquid of Pseudomonas fluorescens (Pseudomonas fluorescens), wax bacillus (Bacillus cereus), Bacillus subtillis (Bacillus subtilis) and 4 kinds of bacterium of bacillus circulans (Bacillus circulans), wherein as follows by weight:
Pseudomonas fluorescens liquid 0.06-0.15 part, wax bacillus liquid 0.04-0.08 part, Bacillus subtillis liquid 0.06-0.10 part, bacillus circulans liquid 0.05-0.08 part;
More than in the fermented liquids of 4 kinds of bacterium living bacteria count respectively all more than 1,000,000,000/milliliter.
2. complex micro organism fungicide, it is characterized in that forming by the fermented liquid of Pseudomonas fluorescens (Pseudomonas fluorescens), wax bacillus (Bacillus cereus), Bacillus subtillis (Bacillus subtilis) and 4 kinds of bacterium of bacillus circulans (Bacillus circulans), wherein as follows by weight:
Pseudomonas fluorescens liquid 0.06-0.15 part, wax bacillus liquid 0.04-0.08 part, Bacillus subtillis liquid 0.06-0.10 part, bacillus circulans liquid 0.05-0.08 part;
More than in the fermented liquids of 4 kinds of bacterium living bacteria count respectively all more than 1,000,000,000/milliliter;
Adopt adsorbents adsorb to become the solid fungicide formulation mixed liquid bacterial agent, described sorbent material is selected the peat composed of rotten mosses for use, one of diatomite or mixing, and the total consumption of sorbent material is (3～10) with the ratio of 4 kinds of bacterium liquid gross weights: 1.
3. the preparation method of claim 1 or 2 described complex micro organism fungicides comprises that the fermented liquid of 4 kinds of bacterium is cultivated activation, shake-flask culture, seeding tank liquid fermentation and culture, the production of fermentor tank liquid fermenting by slant strains respectively, and working condition is as follows:
(1) wax bacillus liquid:
Spawn culture activation and fermention medium all adopt extractum carnis 5 grams, peptone 5 grams, sodium-chlor 5 grams, yeast powder 1 gram, KH
2PO
40.5 gram, MgSO
40.5 1000 milliliters in gram, tap water, pH value 7.0-7.5;
Fermentation culture conditions: culture temperature is 25-28 ℃, and rotating speed is 200 rev/mins, and the volume ratio of air flow and fermented liquid is 1: 1, and unit is v: v/min, is cultured to logarithmic phase;
(2) Pseudomonas fluorescens liquid:
Adopt the LB substratum as spawn culture activatory substratum, liquid nutrient medium is formed: glucose 30 grams, extractum carnis 10 grams, NaNO
33 grams, MgSO
47H
2O 0.3 gram, KCl 0.3 gram, K
2HPO
41.5 1000 milliliters in gram, tap water, pH=6.0-6.5;
Fermentation culture conditions: culture temperature is 23-25 ℃, and rotating speed is 160 rev/mins, and the volume ratio of air flow and fermented liquid is 1: 1, and unit is v: v/min, is cultured to logarithmic phase;
(3) Bacillus subtillis liquid:
Adopt the LB substratum as spawn culture activatory substratum, liquid nutrient medium consists of: 1000 milliliters in peptone 8g, yeast extract paste 5g, glucose 1g, extractum carnis 3g, tap water, pH7.0;
Fermentation culture conditions: culture temperature is 23-25 ℃, and rotating speed is 160 rev/mins, and the volume ratio of air flow and fermented liquid is 1: 1, and unit is v: v/min, is cultured to logarithmic phase;
(4) bacillus circulans liquid:
Adopt the LB substratum as spawn culture activatory substratum, liquid nutrient medium consists of: 1000 milliliters in peptone 5g, yeast extract paste 5g, glucose 10g, extractum carnis 2g, tap water, pH7.0;
The bacillus circulans fermentation culture conditions: culture temperature is 24-28 ℃, and rotating speed is 160 rev/mins, and the volume ratio of air flow and fermented liquid is 1: 1, and unit is v: v/min, is cultured to logarithmic phase;
To be mixed in proportion composite making by the fermented liquid of 4 kinds of bacterium of above method preparation, and perhaps adopt adsorbents adsorb to become the solid fungicide formulation mixed liquid bacterial agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101404007A CN101372666B (en) | 2008-10-17 | 2008-10-17 | Composite microbial preparation for methane tank and preparation thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101404007A CN101372666B (en) | 2008-10-17 | 2008-10-17 | Composite microbial preparation for methane tank and preparation thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101372666A CN101372666A (en) | 2009-02-25 |
| CN101372666B true CN101372666B (en) | 2010-06-30 |
Family
ID=40447045
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008101404007A Expired - Fee Related CN101372666B (en) | 2008-10-17 | 2008-10-17 | Composite microbial preparation for methane tank and preparation thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101372666B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101724560B (en) * | 2009-11-23 | 2011-12-14 | 山东盛隆生物工程有限公司 | Microbial inoculums and preparation method thereof |
| CN102190373B (en) * | 2010-03-08 | 2012-12-26 | 江苏中超环保有限公司 | Aerobic and facultative anaerobic microbial activated bactericide for water treatment and application thereof in contact oxidation process |
| CN101914574B (en) * | 2010-08-02 | 2012-10-24 | 山东省农业科学院土壤肥料研究所 | Method for producing methane through anaerobic fermentation of waste of vegetables and fruits |
| CN102174423B (en) * | 2010-12-06 | 2013-06-19 | 中国农业科学院农业资源与农业区划研究所 | Bacillus licheniformis CH15 for degrading straws and bacterial agent thereof |
| CN102154108A (en) * | 2011-01-19 | 2011-08-17 | 山东省科学院能源研究所 | Pretreatment fungicide for xylose residue or furfural residue, preparation method and application thereof |
| CN102344303A (en) * | 2011-07-01 | 2012-02-08 | 辽宁省微生物科学研究院 | Microbial straw composing agent and preparation method thereof |
| CN102871008A (en) * | 2012-10-11 | 2013-01-16 | 北京大北农科技集团股份有限公司 | Simple preparation method of bacillus bacteria powder |
| CN102924174B (en) * | 2012-11-14 | 2014-07-02 | 河北省科学院生物研究所 | Cucumber growing medium using pig manures and biogas residues as fertilizer sources, and preparation method of cucumber growing medium |
| CN103664254B (en) * | 2013-12-10 | 2015-07-22 | 上海师范大学 | Preparation method of compound microbial fertilizer |
| CN110980942A (en) * | 2019-12-10 | 2020-04-10 | 浙江永续环境工程有限公司 | Anaerobic biological agent and anaerobic treatment method using same |
-
2008
- 2008-10-17 CN CN2008101404007A patent/CN101372666B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN101372666A (en) | 2009-02-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101372666B (en) | Composite microbial preparation for methane tank and preparation thereof | |
| CN101941851B (en) | Technology and process for preparing biochemical humic acid by using kitchen waste | |
| CN103980017B (en) | The method of the quick production high quality organic fertilizer of high-temperature aerobic Secondary Fermentation | |
| CN101775359A (en) | Special microorganism composite bacterial agent for directly decomposing and fermenting crops straws to generate marsh gas and application method thereof | |
| CN103011916B (en) | Method for preparing organic bacteria liquid, organic bacteria liquid prepared by method and application of organic bacteria liquid | |
| CN105733975A (en) | Multifunctional high efficient organic material decomposition agent and preparation method thereof | |
| CN106396817B (en) | Method for preparing organic fertilizer based on straw and livestock and poultry manure | |
| CN106190927B (en) | A kind of bacterial strain and its application for sludge high temperature compost | |
| CN105567612A (en) | Preparation and application of garden waste compound degrading microbial agent | |
| CN102965319B (en) | Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof | |
| CN103484410B (en) | Biological agent for compost treatment of livestock manure | |
| CN1548404A (en) | Production process of microbial organic composite fertilizer | |
| CN103304285A (en) | Microbial agent and preparation method as well as application thereof | |
| CN105948841B (en) | Organic fertilizer tank type fermentation method taking mushroom dregs as substrate | |
| CN102433262B (en) | Complex microbial agent for low-temperature methane fermentation and preparation method thereof | |
| CN101381761B (en) | Application of duel functional composite bacteria agent capable of decomposition of stalk and low-temperature production of biogas | |
| CN103484396A (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN105420169A (en) | Gamma-polyglutamic acid producing strain and method for producing gamma-polyglutamic acid organic fertilizer | |
| CN104193508A (en) | Method for preparing composite organic type environment-friendly seedling culture medium block | |
| CN101864363B (en) | Complex bacterial preparation and application thereof | |
| CN102942420B (en) | Special microbial agent and bio-organic fertilizer for sweet cherry and preparation methods of special microbial agent | |
| CN101109014A (en) | Method for producing hydrogen by dark-light zymolysis couple | |
| CN103396182A (en) | Method for producing solanaceous vegetable seedling growing substrate by utilizing salvaged material of cassava processing | |
| CN101648827A (en) | Fermented biological organic fertilizer and processing method thereof | |
| CN109957527A (en) | A kind of direct decomposing and fermenting crops straws produce special microorganism composite bacterial agent preparation and its application method of biogas |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100630 Termination date: 20141017 |
|
| EXPY | Termination of patent right or utility model |