CN101372424B

CN101372424B - Method for preparing humic acid bio-fertilizer from lignite

Info

Publication number: CN101372424B
Application number: CN200810137108XA
Authority: CN
Inventors: 滕春红; 谢吉旺; 陈成
Original assignee: HEILONGJIANG HONGDA BIOLOGICAL ENGINEERING Co Ltd
Current assignee: HEILONGJIANG HONGDA BIOLOGICAL ENGINEERING Co Ltd
Priority date: 2008-09-12
Filing date: 2008-09-12
Publication date: 2011-03-16
Anticipated expiration: 2028-09-12
Also published as: CN101372424A

Abstract

The invention provides a method for preparing a biological fertilizer by brown coal humic acid. With phanerochaete chrysosporium CICC40719 as a starting strain; 100-300ml solution containing 0.3% of KH2PO4, 0.15%g of MgSO4 7H2O, 0.01% of vitamin B1, 5% of pulverized brown coal and 94.54% of deionized water is inoculated with the phanerochaete chrysosporium, which is cultured by a shaking table atthe temperature of 28 DEG C for 96 hours to prepare a bacterial culture suspension I; another 100-300ml solution containing 0.3% of KH2PO4, 0.15%g of MgSO4 7H2O, 0.01% of vitamin B1, 10% of pulverized brown coal and 89.54% of deionized water is inoculated with 5% of the bacterial culture suspensionI to prepare a bacterial culture suspension II; another 100-300ml solution containing 0.3% of KH2PO4, 0.15%g of MgSO4 7H2O, 0.01% of vitamin B1, 15% of pulverized brown coal and 84.54% of deionized water is inoculated with 5% of the bacterial culture suspension II which is cultured by the shaking table to prepare a bacterial culture suspension III which is separated and purified to obtain a phanerochaete chrysosporium acclimatized strain. A culture medium is inoculated with the culture medium and cultured for 24 hours, and fermentation broth is prepared. The brown coal is pulverized by a pulverizer and sieved by a 30-mesh sieve to obtain pulverized coal, which is added with water for regulating the moisture content of the pulverized coal to 35-40%, 12-15% of ammonia water (wet basis), and 2% of rice hull (wet basis), the wet pulverized coal is stirred evenly, spread out indoors to a thickness of 8cm, and thermostatically cultured at the temperature of 28 DEG C for 30 hours, then sulfuric acid solution with 8% percentage concentration is used for neutralizing the degraded pulverized brown coal, the pH value of which is adjusted between 6.5 and 7.5, after being dried with hot air at the temperature of 55 DEG C and pulverized, the biological fertilizer is prepared.

Description

With brown coal is the method for feedstock production humic acid bio-fertilizer

(1) technical field

The present invention relates to a kind of is raw material prepares humic acid bio-fertilizer behind microbial fermentation method with brown coal.

(2) background technology

Brown coal are low because of its calorific value, and industrial using value is less, is referred to as low grade coal.At present, the output of China's brown coal and reserves are all bigger, and therefore, the development and use of brown coal just become the new problem of current coal-mining industry research.In recent years, exploring the new way of development and use low grade coal both at home and abroad.Because be rich in humic acid in the brown coal molecular composition, and the effect of the too busy to get away soil microorganisms of the formation of humic acid in the coal, therefore, coal can be decomposed by Institute of Micro-biology too.In order to study the degradation problem of microorganism to brown coal, researcher was once isolated many microbial strainss from soil and brown coal both at home and abroad, and the humic acid in humic acid and the weathered coal is changed.Usually the material that contains a large amount of lignin structures in the brown coal with the microorganism of the lignin degrading brown coal of degrading, just might therefrom obtain the chemical that some have special value.These chemical are high water solubles, contain oxygen and nitrogen-containing functional group on its aromatic structure more, have the effect of regulating soil property, promoting plant-growth and stabilize water coal slurry, and can be used for as immunoadjuvant and sewage-treating agent etc.

It is starting strain that this project adopts the Phanerochaete chrysosporium in the white-rot fungi, and through enrichment culture, domestication obtains superior strain, has opened up one for the microbiological deterioration and the utilization research of brown coal and has realized that the energy is benign, the new road of Sustainable development.This has important contribution and far reaching significance to the sound development of safeguarding the whole world under the urgent day by day global form of current energy dilemma and environmental protection problem.Though the liquefaction of the microorganism of brown coal utilizes research still to be in the initial stage at present, there are many problem expectations to solve, but compare with traditional industrial method for transformation, have that energy consumption is low, conversion condition is gentle, transformation efficiency is high, a series of superiority such as the economic benefit of converted product, using value height, equipment requirements are simple, and be subject to people's attention day by day and pay close attention to, its research is just in the ascendant.

(3) summary of the invention

The object of the invention is to provide a kind of preparation method of brown coal humic acid bio-fertilizer.

The object of the present invention is achieved like this: related per-cent is mass ratio except that other has indicating among the present invention, and product of the present invention adopts such method to prepare:

At first detect brown coal moisture, pulverizer was pulverized 30 mesh sieves then, add water and adjust coal dust moisture 35-40%, add ammoniacal liquor 12-15% (wet basis), rice husk 2% (wet basis), inoculate Phanerochaete chrysosporium (purchase in Chinese industrial microbial strains preservation center and be numbered CICC40719) fermented liquid 10% then, the coal dust that will wet stirs, indoorly spread out to 8cm thickly, 28 ℃ of heat insulating culture 30 hours are in 8% the sulfuric acid liquid and the brown coal powder after the degraded with percentage concentration then, the sulphuric acid soln consumption accounts for 10% of enzyme powder wet basis greatly, adjust between the coal dust pH6.5--7.5, after 55 ℃ of warm air dryings, pulverize and make the brown coal humic acid bio-fertilizer.

The present invention also has some technical characterictics like this:

1. described Phanerochaete chrysosporium fermented liquid preparation: W-Gum 5%, glucose 2%, KH ₂PO ₄0.3%, MgSO ₄.7H ₂O 0.15%, vitamins B ₁O.01%, deionized water 92.54% was sterilized 10-30 minute for substratum 110-150 ℃, and inoculation Phanerochaete chrysosporium naturalized strain ventilates and cultivated 24 hours, the preparation fermented liquid.

2. described Phanerochaete chrysosporium answers preconcentration to cultivate domestication, and the enrichment acclimation method is:

2.1. 100-300ml is comprised KH ₂PO ₄0.3%, MgSO ₄.7H ₂O 0.15% gram, vitamins B ₁O.01%, brown coal coal dust 5% deionized water 94.54% was sterilized 10-30 minute for substratum 110-150 ℃, the inoculation Phanerochaete chrysosporium, and 28 ℃ of shaking tables were cultivated 96 hours, made thalline suspension I;

2.2. 100-300ml is comprised KH ₂PO ₄0.3%, MgSO ₄.7H ₂O 0.15% gram, vitamins B ₁O.01%, brown coal coal dust 10%, deionized water 89.54% was sterilized 10-30 minute for substratum 110-150 ℃, inoculated 5%, 28 ℃ of shaking table of above-mentioned thalline suspension I and cultivated 96 hours, made thalline suspension II;

2.3. 100ml-300ml is comprised KH ₂PO ₄0.3%, MgSO ₄.7H ₂O 0.15% gram, vitamins B ₁O.01%, brown coal coal dust 15%, deionized water 84.54%, sterilized 10-30 minute for substratum 110-150 ℃, inoculate 5%, 28 ℃ of shaking table of above-mentioned thalline suspension II and cultivated 96 hours, make thalline suspension III, finish enrichment domestication process, separation and purification 2.3 step thalline suspensions, obtain can the acid hydrolysis brown coal the Phanerochaete chrysosporium naturalized strain.

3. described shaking table is 150 rev/mins.

4. effect test

Choose cucumber, pumpkin, paddy rice for the indication crop, carry out pot experiment, verify this bacterial manure effect.

Product of the present invention is pressed 75g/m ², consumption be applied to indication crop pot experiment, indication plant growth situation is seen description of drawings after emerging 15 days.

Lignite humic acid and soil rotten phytic acid have similar part so lignite humic acid to be subjected to the attention of countries in the world in the applied research of agriculture field in the influence of forming, character reaches soil and crop always.From the sixties, states such as U.S., day, Soviet Union (the former Soviet Union), method, Austria have set up the factory of humic acid fertilizer in succession, and wherein with the fastest developing speed with the former Soviet Union and Japan, scale is maximum also.China utilizes the brown coal composting to start from the latter stage fifties, extracts fulvic acid from brown coal, carries out agricultural application and studies over past ten years and make outstanding achievements, and has caused the concern of world many countries, and some achievement in research obtains the attention and the high evaluation of international academic community.

(4) description of drawings

Fig. 1 from left to right is followed successively by the amount of application 75g/m of humic acid combined fertilizer for indication crop pumpkin growing state after emerging 15 days in the humic acid combined fertilizer that uses different amounts among Fig. 1 ², the amount of application 30g/m of humic acid combined fertilizer ², contrast.Fig. 2 for indication crop paddy rice use the humic acid combined fertilizer emerged 15 days after growing state, from left to right be followed successively by the amount of application 75g/m of humic acid combined fertilizer among Fig. 2 ², contrast.

(5) embodiment

(5) embodiment

For a more detailed description below in conjunction with specific embodiment to the present invention:

1.1. fermented bacterium source

Phanerochaete chrysosporium is purchased in Chinese industrial microbial strains preservation administrative center in the present embodiment, and bacterium numbering CICC40719 is used to produce the xylogen lytic enzyme.

1.2 strain domestication

1.2.1. 100-300ml is comprised KH ₂PO ₄0.3%, MgSO ₄.7H ₂O 0.15% gram, vitamins B ₁O.01%, brown coal coal dust 5% deionized water 94.54%.Sterilized 10-30 minute for substratum 110-150 ℃, the inoculation Phanerochaete chrysosporium, 28 ℃ of shaking tables were cultivated 96 hours, made thalline suspension I;

1.2.2. 100-300ml is comprised KH ₂PO ₄0.3%, MgSO ₄.7H ₂O 0.15% gram, vitamins B ₁O.01%, brown coal coal dust 10% was sterilized 10-30 minute for deionized water 89.54% substratum 110-150 ℃, inoculated 5%, 28 ℃ of shaking table of above-mentioned thalline suspension I and cultivated 96 hours, made thalline suspension II;

1.2.3. 100ml-300ml is comprised KH ₂PO ₄0.3%, MgSO ₄.7H ₂O 0.15% gram, vitamins B ₁O.01%, brown coal coal dust 15%, sterilized 10-30 minute for deionized water 84.54% substratum 110-150 ℃, inoculate above-mentioned thalline suspension II 5%, 28 ℃ of shaking tables were cultivated 96 hours, made thalline suspension III, finished enrichment domestication process, separation and purification 2.3 step thalline suspensions, obtain can the acid hydrolysis brown coal the Phanerochaete chrysosporium naturalized strain.

1.3 the preparation of brown coal humic acid bio-fertilizer

At first detect brown coal moisture, pulverizer was pulverized 30 mesh sieves then, add water and adjust coal dust moisture 35-40%, add ammoniacal liquor 12-15% (wet basis), rice husk 2% (wet basis), inoculate Phanerochaete chrysosporium (purchase in Chinese industrial microbial strains preservation center and be numbered CICC40719) fermented liquid 10% then, the coal dust that will wet stirs, indoorly spread out to 8cm thickly, 28 ℃ of heat insulating culture 30 hours are in 8% the sulfuric acid liquid and the brown coal powder after the degraded with percentage concentration then, the sulphuric acid soln consumption accounts for 10% of enzyme powder wet basis greatly, adjust between the coal dust pH6.5-7.5, after 55 ℃ of warm air dryings, pulverize and make the brown coal humic acid bio-fertilizer.

Claims

1. be the method for feedstock production humic acid bio-fertilizer with brown coal, it is characterized in that making by following preparation method:

1) domestication is cultivated in the Phanerochaete chrysosporium preconcentration, and the enrichment acclimation method is:

1.1). 100-300ml is comprised KH ₂PO ₄0.3%, MgSO ₄.7H ₂O 0.15%, vitamins B ₁0.01%, brown coal coal dust 5%, deionized water 94.54%, sterilized 10-30 minute for substratum 110-150 ℃, the Phanerochaete chrysosporium that is numbered CICC40719 in Chinese industrial microbial strains preservation center is purchased in inoculation, and 28 ℃ of shaking tables were cultivated 96 hours, made thalline suspension I;

1.2). 100-300ml is comprised KH ₂PO ₄0.3%, MgSO ₄.7H ₂O 0.15%, vitamins B ₁0.01%, brown coal coal dust 10%, deionized water 89.54% was sterilized 10-30 minute for substratum 110-150 ℃, inoculated above-mentioned thalline suspension I5%, and 28 ℃ of shaking tables were cultivated 96 hours, made thalline suspension II;

1.3). 100ml-300ml is comprised KH ₂PO ₄0.3%, MgSO ₄.7H ₂O 0.15%, vitamins B ₁0.01%, brown coal coal dust 15%, deionized water 84.54%, sterilized 10-30 minute for substratum 110-150 ℃, inoculate above-mentioned thalline suspension II5%, 28 ℃ of shaking tables were cultivated 96 hours, make thalline suspension III, finish enrichment domestication process, this step of separation and purification thalline suspension, obtain can the acid hydrolysis brown coal the Phanerochaete chrysosporium naturalized strain;

2) Phanerochaete chrysosporium fermented liquid preparation:

W-Gum 5%, glucose 2%, KH ₂PO ₄0.3%, MgSO ₄.7H ₂O 0.15%, vitamins B ₁0.01%, deionized water 92.54% was sterilized 10-30 minute for substratum 110-150 ℃, and inoculation Phanerochaete chrysosporium naturalized strain ventilates and cultivated 24 hours, the preparation fermented liquid;

3) preparation of humic acid bio-fertilizer:

Brown coal are pulverized through pulverizer, cross 30 mesh sieves, add water and adjust coal dust moisture 35-40%, add ammoniacal liquor 12-15%, rice husk 2% is inoculated Phanerochaete chrysosporium fermented liquid 10% then, and the coal dust that will wet stirs, indoor spread out to 8cm thick, 28 ℃ of heat insulating culture 30 hours are in 8% the sulfuric acid liquid and the brown coal powder after the degraded with percentage concentration then, adjust between the coal dust pH6.5-7.5, after 55 ℃ of warm air dryings, pulverize and make the brown coal humic acid bio-fertilizer.