CN101361810B - Traditional Chinese medicine combination for treating virus lower respiratory tract infection and preparation method thereof - Google Patents
Traditional Chinese medicine combination for treating virus lower respiratory tract infection and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese drug composition used for the treatment of viral lower respiratory infection and a preparation method thereof. The traditional Chinese drug composition is prepared by Herba Ajugae, polygonum cuspidatum and integripetal rhodiola herb, or prepared by Herba Ajugae extract, polygonum cuspidatum extract and integripetal rhodiola herb extract. The traditional Chinese drug composition has the obvious functions of relieving fever and cooling as well as anti-inflammatory, and also has the obvious curative effect of the treatment of virus pneumonia.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition and preparation method thereof, particularly Chinese medicine composition of treatment viral lower respiratory infection and preparation method thereof.
Background technology
Respiratory tract infection is human modal illness.All having 90%～95% in the acute respiratory infection, due to virus, its importance far surpasses antibacterial and other microorganisms, China dies from respiratory tract infection every year just 1,500,000 people, adds that the concurrent respiratory tract infection of other basic diseases causes the dead and surpasses 3,000,000 people.Current hospital mortality the highest be pulmonary infection.The first two critically ill patient such as cerebrovascular, tumor of mortality rate at last can most of complicated with pulmonary infection, and directly dies from viral or fungal pneumonia.2000 the whole world best-selling the last 500 medicine in, the sales volume of antiviral drugs is 7,300,000,000 dollars, accounts for 30% of anti-infection drug.However, Western medicine is unsatisfactory for the effect of virus, and such disease generation is frequent, and Susceptible population is extensive, lacks specific effective Therapeutic Method at present again, is still the major disease of the health that has had a strong impact on people so far.
The doctor trained in Western medicine clinical treatment present situation of viral pneumonia and the problem of existence: the viral infection of respiratory tract is very common, accounts for 90% of acute respiratory infection, but mainly based on upper respiratory tract infection.Lower respiratory infection (the being viral pneumonia) sickness rate in recent years of virus also has the trend that increases, show according to Study on etiologic agents, it accounts for 18.18% of CAP, account for 5% in the same period in the hospital acquired infection, especially in preceding HIV sufferers because of infected person para-immunity defective virus (HIV), its sickness rate sharply raises especially, more and more is subjected to people's attention.
The main virus that causes viral pneumonia has: viral pneumonia virus, respiratory syncytial virus, adenovirus, rhinovirus, secondary viral pneumonia virus, myxovirus and some enterovirus such as echovirus, Coxsackie virus and Epstein-Barr virus, the initial upper respiratory tract infection that often shows as of above virus, lesion growth spreads downwards later on, involves bronchus and lung; Another kind of virus such as chickenpox virus, Measles virus, cytomegalovirus (CMV), herpes simplex virus, varicella zoster virus etc. are often invaded lungs such as skin, central nervous system earlier and are organized outward, reach lung by approach such as blood then, cause viral pneumonia.The life-time service broad ectrum antibiotic can make the former virus that colonizes in upper respiratory tract cause pneumonia (as cytomegalovirus, varicella zoster virus etc.); Eruption sexually transmitted disease (STD) viral disease, but secondary such as chickenpox, measles toxicity pneumonia.
Viral pneumonia, the traditional Chinese medical science are thought and are belonged to wind-warm lung-heat disease.Chinese medicine has the mechanism different with the Western medicine of inhibition, kill virus at anti-virus aspect, be immunologic function, directly many target spots, multipath comprehensive function such as kill virus, antagonism morbid poison, thereby reach the purpose that alleviates the state of an illness, the shortening course of disease, reduces mortality rate by influencing the people.Therefore people extensively are devoted to develop the Chinese patent medicine with antivirus action.Occupy in this sick new Chinese medicine of treatment at present the market share bigger as antivirus oral liquid, Radix Isatidis granules, SHUANGHUANGLIAN KOUFUYE, QINGRE JIEDU KOUFUYE, antitoxic bolus of honeysuckle flower and forsythia (sheet) etc., basically be used for the respirovirus sexuality and dye, but antivirus action is not strong.
China classifies viral pneumonia as common refractory anxious critical illness.Therefore, the seek treatment active drug of viral pneumonia (wind-warm lung-heat) is pressing for of clinical practice.
Summary of the invention
First purpose of the present invention is to disclose a kind of Chinese medicine composition for the treatment of viral lower respiratory infection.
Second purpose of the present invention is to disclose a kind of preparation method for the treatment of the Chinese medicine composition of viral lower respiratory infection.
The 3rd purpose of the present invention is to disclose a kind of method for preparing drop pills for the treatment of the Chinese medicine composition of viral lower respiratory infection.
First purpose of the present invention is achieved by the following technical solution:
Option A:
A kind of Chinese medicine composition for the treatment of viral lower respiratory infection, make by the raw material of following weight ratio:
Herba Ajugae 5-25 weight portion, Rhizoma Polygoni Cuspidati 3-20 weight portion, Radix Rhodiolae 1-12 weight portion;
The Chinese medicine composition of described treatment viral lower respiratory infection, preferably make by the raw material of following weight ratio:
Herba Ajugae 10-20 weight portion, Rhizoma Polygoni Cuspidati 8-15 weight portion, Radix Rhodiolae 3-8 weight portion;
The Chinese medicine composition of described treatment viral lower respiratory infection, preferably make by the raw material of following weight ratio:
Herba Ajugae 15 weight portions, Rhizoma Polygoni Cuspidati 10 weight portions, Radix Rhodiolae 5 weight portions;
Can further add Pulvis Fellis Suis 0.1-1 weight portion in the Chinese medicine composition of the treatment viral lower respiratory infection described in the option A.
Option b:
The inventor finds that further Rhizoma Polygoni Cuspidati in the above-mentioned composition is replaced with Radix Scutellariae can realize above-mentioned technical purpose equally, and possesses same technique effect.
Therefore, the present invention provides a kind of Chinese medicine composition for the treatment of viral lower respiratory infection in addition, is made by the raw material of following weight ratio:
Herba Ajugae 5-25 weight portion, Radix Scutellariae 3-20 weight portion, Radix Rhodiolae 1-12 weight portion;
The Chinese medicine composition of described treatment viral lower respiratory infection, preferably make by the raw material of following weight ratio:
Herba Ajugae 10-20 weight portion, Radix Scutellariae 8-15 weight portion, Radix Rhodiolae 3-8 weight portion;
The Chinese medicine composition of described treatment viral lower respiratory infection, preferably make by the raw material of following weight ratio:
Herba Ajugae 15 weight portions, Radix Scutellariae 10 weight portions, Radix Rhodiolae 5 weight portions;
Can further add Pulvis Fellis Suis 0.1-1 weight portion in the Chinese medicine composition of the treatment viral lower respiratory infection described in the option b.
Above-mentioned a kind of preparation method for the treatment of the Chinese medicine composition of viral lower respiratory infection can be selected any one in the following method for use:
1, gets Herba Ajugae 5-25 weight portion, Rhizoma Polygoni Cuspidati 3-20 weight portion (or Radix Scutellariae 3-20 weight portion), Radix Rhodiolae 1-12 weight portion, ethanol extraction 2-3 time that adds 60-80%, the 4-8 that each ethanol consumption is the medical material amount is (weight ratio) doubly, the each extraction 1-2 hour, merge extractive liquid,, reclaim ethanol to there not being the alcohol flavor, promptly get extracting solution;
2, get Herba Ajugae 5-25 weight portion, Rhizoma Polygoni Cuspidati 3-20 weight portion (or Radix Scutellariae 3-20 weight portion), Radix Rhodiolae 1-12 weight portion, ethanol extraction 2-3 time that adds 60-80%, the 4-8 that each ethanol consumption is the medical material amount is (weight ratio) doubly, extracts merge extractive liquid, 1-2 hour at every turn, reclaim ethanol to there not being the alcohol flavor, promptly get extracting solution; Said extracted liquid is filtered, and filtrate is by macroporous resin, and with the ethanol elution of 4-6 times of water gaging or 20% following concentration, eluent is abandoned or adopted earlier, and reuse adds 1-10 times of eluting of 50-80% ethanol, the collection eluent, and decompression recycling ethanol gets the purification extracting solution to there not being the alcohol flavor.
The preparation method of the Chinese medicine composition of above-mentioned a kind of treatment viral lower respiratory infection can be made through the extraction process of routine [as model Bi Ting " pharmacy of Chinese materia medica " (Shanghai Science Press 1997 December the 1st edition)] by the above-mentioned raw materials medicine.
Above-mentioned Herba Ajugae, Rhizoma Polygoni Cuspidati, Radix Rhodiolae can be made the acceptable any conventional dosage form of pharmaceutics by preparation process routinely after extracting, comprise capsule, tablet, granule, gel, slow releasing agent, oral liquid, spray, patch.
For above-mentioned dosage form can be realized, need when these dosage forms of preparation, to add the pharmacy acceptable auxiliary, for example: filler, disintegrating agent, lubricant, suspending agent, binding agent, sweeting agent, correctives, antiseptic, substrate etc.Filler comprises: starch, pregelatinized Starch, lactose, mannitol, chitin, microcrystalline Cellulose, sucrose etc.; Disintegrating agent comprises: starch, pregelatinized Starch, microcrystalline Cellulose, carboxymethyl starch sodium, crospolyvinylpyrrolidone, low-substituted hydroxypropyl cellulose, cross-linking sodium carboxymethyl cellulose etc.; Lubricant comprises: magnesium stearate, sodium lauryl sulphate, Pulvis Talci, silicon dioxide etc.; Suspending agent comprises: polyvinylpyrrolidone, microcrystalline Cellulose, sucrose, agar, hydroxypropyl emthylcellulose etc.; Binding agent comprises, starch slurry, polyvinylpyrrolidone, hydroxypropyl emthylcellulose etc.; Sweeting agent comprises: saccharin sodium, Aspartane, sucrose, cyclamate, enoxolone etc.; Correctives comprises: sweeting agent and various essence; Antiseptic comprises: parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, fixed, the Folium eucalypti globueli (Eucalyptus globulus Labill.) wet goods of acetic acid chloroethene; Substrate comprises: PEG6000, PEG4000, insect wax etc.For making above-mentioned dosage form can realize pharmacy of Chinese materia medica, need when these dosage forms of preparation, to add acceptable other adjuvant of pharmacy (adjuvant of each dosage form record among the Fan Biting " pharmacy of Chinese materia medica ", Shanghai Science Press December in 1997 the 1st edition).
The Chinese medicine composition of treatment viral lower respiratory infection of the present invention is except the form that feeds intake with Herba Ajugae, Rhizoma Polygoni Cuspidati, Radix Rhodiolae crude drug, the form that can also adopt the extract (effective site) with Herba Ajugae, Rhizoma Polygoni Cuspidati (or Radix Scutellariae), Radix Rhodiolae to feed intake, thereby make targeting, target spot clearer and more definite, therefore the present invention further discloses the Chinese medicine composition (square case C and D) of treatment viral lower respiratory infection:
Scheme C:
A kind of Chinese medicine composition for the treatment of viral lower respiratory infection, said composition is made by the crude drug of following weight ratio:
Herba Ajugae extract 5-25 weight portion, Rhizoma Polygoni Cuspidati extract 3-20 weight portion, Radix Rhodiolae extract 1-12 weight portion;
The Chinese medicine composition of described treatment viral lower respiratory infection, preferably make by the raw material of following weight ratio:
Herba Ajugae extract 10-20 weight portion, Rhizoma Polygoni Cuspidati extract 8-15 weight portion, Radix Rhodiolae extract 3-8 weight portion;
The Chinese medicine composition of described treatment viral lower respiratory infection, preferably make by the raw material of following weight ratio:
Herba Ajugae extract 15 weight portions, Rhizoma Polygoni Cuspidati extract 10 weight portions, Radix Rhodiolae extract 5 weight portions;
Can further add Pulvis Fellis Suis 2.5-25 weight portion in the Chinese medicine composition of the treatment viral lower respiratory infection described in the scheme C.
Scheme D:
A kind of Chinese medicine composition for the treatment of viral lower respiratory infection, said composition is made by the crude drug of following weight ratio:
Herba Ajugae extract 5-25 weight portion, Radix Scutellariae extract 3-20 weight portion, Radix Rhodiolae extract 1-12 weight portion;
The Chinese medicine composition of described treatment viral lower respiratory infection, preferably make by the raw material of following weight ratio:
Herba Ajugae extract 10-20 weight portion, Radix Scutellariae extract 8-15 weight portion, Radix Rhodiolae extract 3-8 weight portion;
The Chinese medicine composition of described treatment viral lower respiratory infection, preferably make by the raw material of following weight ratio:
Herba Ajugae extract 15 weight portions, Radix Scutellariae extract 10 weight portions, Radix Rhodiolae extract 5 weight portions;
Can further add Pulvis Fellis Suis 2.5-25 weight portion in the Chinese medicine composition of the treatment viral lower respiratory infection described in the scheme D.
Herba Ajugae extract of the present invention can be but be not limited to alcohol extraction and cross the refining thing of post, water and proposed any one or a few combination in post refining thing, ethanol extract or the water extract; Luteolin content must not be less than 1.0% in the described Herba Ajugae extract, and general flavone content must not be less than 10.0%, and total alkaloid content must not be less than 5.0%, and total phenolic content must not be less than 15%;
Rhizoma Polygoni Cuspidati extract of the present invention can be but be not limited to alcohol extraction and cross the refining thing of post, water and proposed any one or a few combination in post refining thing, ethanol extract or the water extract; The total anthraquinones content sum must not be less than 15% in the described Rhizoma Polygoni Cuspidati extract, and polydatin content must not be less than 5.5%, and Resveratrol content must not be less than 0.50%, and emodin content must not be less than 2.0%;
Radix Rhodiolae extract of the present invention can be but be not limited to alcohol extraction and cross the refining thing of post, water and proposed any one or a few combination in post refining thing, ethanol extract or the water extract; Wherein general flavone content is no less than 20%, and the content of rhodioside must not be less than 2.0%, 70% ethanol soluble extraction must not be less than 60%.
Radix Scutellariae extract of the present invention can be but be not limited to alcohol extraction and cross the refining thing of post, water and proposed any one or a few combination in post refining thing, ethanol extract or the water extract; Wherein content of baicalin must not be less than 85.0% in the Radix Scutellariae extract;
Hyodeoxycholic Acid must not be less than 16.0% in the described Pulvis Fellis Suis.
The preparation method of the Chinese medicine composition of the described treatment viral lower respiratory infection of making by Herba Ajugae, Rhizoma Polygoni Cuspidati, Radix Rhodiolae extract (effective site), wherein Herba Ajugae extract, Rhizoma Polygoni Cuspidati extract, Radix Rhodiolae extract can be by commercially available purchase, make the acceptable any conventional dosage form of pharmaceutics according to above-mentioned prescription ratio and preparation process routinely, comprise drop pill, capsule, tablet, granule, gel, slow releasing agent, oral liquid;
Herba Ajugae extract of the present invention, Rhizoma Polygoni Cuspidati extract (or Radix Scutellariae extract), the concrete alcohol extraction of Radix Rhodiolae extract cross the refining thing of post, water carried the refining thing of post, ethanol extract or water extract can but be not limited to by following method preparation:
A, Herba Ajugae preparation method of extract:
Get the ethanol that Herba Ajugae adds 60-80%, reflux, extract, 3-4 time, each 1-2 hour, the solvent consumption was 4-6 a times of medical material amount, merge extractive liquid, gets the Herba Ajugae extracting solution; Or
Get Herba Ajugae and use water extraction 2-3 times, each 1-2 hours, the solvent consumption was 4-6 a times of medical material amount.Merge extractive liquid,, being concentrated into 50-60 ℃ of relative density is 1.1-1.3, adds ethanol and makes concentration of alcohol reach 75%, cold preservation 10-48 hour, filtration added ethanol and makes concentration of alcohol reach 85%, and cold preservation 24-36 hour, filter to clarification, get the Herba Ajugae extracting solution.
The preparation method of b, Rhizoma Polygoni Cuspidati extract:
Get Rhizoma Polygoni Cuspidati and decoct with water extraction 3-4 time, each 1-2 hour, the solvent consumption was 4-6 a times of medical material amount, merge extractive liquid,, being concentrated into 50-60 ℃ of relative density is 1.1-1.3, adds ethanol and makes concentration of alcohol reach 70%, cold preservation 24-36 hour, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 80% to there not being the alcohol flavor, cold preservation 24-36 hour, filtration is reclaimed ethanol to there being the ethanol flavor to clarification, gets the Rhizoma Polygoni Cuspidati extracting solution; Or
Get the ethanol that Rhizoma Polygoni Cuspidati adds 60-80%, reflux, extract, 3-4 times, each 1-2 hour, the solvent consumption was 4-6 a times of medical material amount, merge extractive liquid, gets the Rhizoma Polygoni Cuspidati extracting solution.
The preparation method of c, Radix Rhodiolae extract:
Get the ethanol that Radix Rhodiolae adds 60-80%, reflux, extract, 3-4 time, each 1-2 hour, the solvent consumption was 4-6 a times of medical material amount, merge extractive liquid, gets Radix Rhodiolae extracting solution.
D, Herba Ajugae extract, Rhizoma Polygoni Cuspidati extract, Radix Rhodiolae extract are crossed the method that post extracts
Above-mentioned Herba Ajugae, Rhizoma Polygoni Cuspidati, Radix Rhodiolae extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gaging or 20% following concentration, eluent is abandoned or adopted, reuse adds 3-10 times of eluting of 60-90% ethanol, the collection eluent, decompression recycling ethanol gets the purification extracting solution to there not being the alcohol flavor.
The preparation method of e, Radix Scutellariae extract:
Extracting in water 3 times adds 12 times of amounts of water at every turn, extracts 0.5 hour at every turn.The effective site process for purification is defined as: under 80 ℃, transfer pH to 1-2, be incubated 15 minutes, filter, precipitate adds 2 times of water and stirs, and regulates more than the pH to 7 with 40% sodium hydroxide solution, 95% the ethanol that adds equivalent stirs and makes dissolving, filters, filtrate is regulated pH to 1-2 with hydrochloric acid, 60 ℃ are incubated 15 minutes, leave standstill, and filter, precipitate water and Different concentrations of alcohol washing are to neutral, and drying under reduced pressure promptly.
The present invention further discloses a kind of extraction Herba Ajugae method, this method comprises the steps:
70% ethanol extraction twice was measured reflux, extract, 2 hours with 10 times for the first time, extracted 1.5h with 8 times of amounts for the second time; Extracting solution filters, merge extracting solution A;
Get the centrifugal 30min of extracting solution A, get supernatant B and collect residue C;
Supernatant B crosses post: adopt the AB-8 resin, last sample (supernatant B) concentration 0.2g/ml, absorption flow velocity 2BV/h, wash with water earlier, volume is 3BV (column volume doubly), is 20% ethanol elution 5BV and 70% ethanol elution 5BV with eluant successively again, and elution flow rate is 3BV/h;
The eluent of 20% and 70% part is merged with residue C, reclaim ethanol, vacuum drying.
Total phenolic acid, content of total flavone can reach more than 25%.
The present invention further discloses a kind of extraction Rhizoma Polygoni Cuspidati method, this method comprises the steps:
70% ethanol extraction 3 times, each 1.5 hours, 8 times of amounts; Extracting solution filters, merge extracting solution a;
The centrifugal 30min of extracting solution a gets supernatant, and collects residue.
Supernatant is crossed post: adopt the D-101 resin, last sample concentration 0.15g/ml, absorption flow velocity 2.4BV/h, wash with water earlier, volume is 2BV, reuse 20% pure eluting 5BV, last is 60% ethanol and 90% ethanol with eluant successively, and the eluant consumption is 5BV, and elution flow rate is 3BV/h.
60% and 90% eluent is merged with residue, reclaim ethanol, vacuum drying.Total anthraquinones content can reach more than 15%.
The present invention further discloses a kind of extraction Radix Rhodiolae method, this method comprises the steps:
The Radix Rhodiolae medical material adds the water of 8 times of medical material amounts, extracts 3 times, each 60min; Extracting solution filters, merging filtrate;
The centrifugal 30min of filtrate gets supernatant;
Supernatant is crossed post: adopt the AB-8 macroporous resin column, and last sample concentration (supernatant concentration) 0.2g/ml, absorption flow velocity 2.5BV/h, washing volume 3BV, to adopt 80% ethanol elution 5BV behind distilled water, 10% ethanol successively eluting 5BV, elution flow rate is 3BV/h.80% eluent is reclaimed ethanol; Eluent is dissolved in water, and adopts 10000 ultrafilter membrane ultrafiltration, the filtrate concentrate drying.General flavone content can reach more than 20%.
The present invention further discloses the preferred for preparation method of above-mentioned Chinese medicine composition dropping pill formulation:
Exsiccant medicine is pulverized and is fine powder, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is more than the 1:1.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.
Pharmaceutical composition of the present invention (option A, option b) possesses tangible analgesic cooling effect, antiinflammatory action, and is evident in efficacy to the treatment viral pneumonia.
The Chinese medicine composition (scheme C, scheme D) of the form preparation that the present invention feeds intake with extract makes targeting, target spot clearer and more definite, and outstanding effect is concentrated one's efforts grand, possesses tangible analgesic cooling effect, antiinflammatory action, and is evident in efficacy to the treatment viral pneumonia.
Rhizoma Polygoni Cuspidati disclosed by the invention, Radix Rhodiolae, the preferred extracting method of Herba Ajugae, the active constituents of medicine of extraction is many, good drug efficacy.
Following experimental example and embodiment do further explanation to the present invention, but are not construed as limiting the invention.
The lung heat clearing of detoxifcation described in following experimental example drop pill is the prepared product of embodiment 1 method.
Experimental example 1 clinical experiment
1 clinical data
64 examples are inpatient, all meet the viral pneumonia diagnostic criteria; Be divided into treatment group and matched group at random.
64 examples are organized in treatment, male 30 examples, women 34 examples; Minimum 11 years old of age, maximum 70 years old; 37.5～38 ℃ of body temperature, 38 examples; 38.1～39 ℃, 20 examples; 39.1～40 ℃; 6 examples.
Matched group 64 examples, male 31 examples, women 33 examples; Minimum 13 years old of age, maximum 69 years old; 37.5～38 ℃ of body temperature, 40 examples; 38.1～39 ℃, 19 examples; 39.1～40 ℃, 5 examples.
Two groups of case baseline data are learned processing by statistics, there was no significant difference, (P〉0.05).
2 clinical manifestations
2.1 epidemiological history
A large amount of heat generation respiratory tract infection patients or fever clinic appear in autumn and winter season, emergency treatment heat generation respiratory tract infection patient obviously increases.
2.2 clinical primary symptom and lab testing
Primary symptom: acute onset, symptoms such as heating, fear of cold, pharyngalgia, dizziness, headache, systemic pain, cough, red tongue, yellow fur, floating and rapid pulse.
Lab testing: the not high or attenuating of peripheral hemogram total white blood cells, lymphocyte increases relatively.
The X ray examination: one-sided or bilateral lung has netted shade, and increased bronchovascular shadows is fuzzy.Other febrile diseases in severe patient two lungs, except the down visible diffusivity nodositas infiltration.
Western medicine diagnose is a viral pneumonia;
Chinese medical discrimination belongs to the wind-warm lung-heat disease that pyretic toxicity accumulates lung.
3 criterions of therapeutical effect
Curative effect judging standard is formulated with reference to " new Chinese medicine treatment clinical research guideline ".
Clinical recovery: treat 7 days normal with interior temperature recovery, clinical symptom disappearance, the auscultation pulmonary rale disappears; X-ray examination two lungs are normal, and the hemogram chemical examination is normal;
Produce effects: treat in 7 days, body temperature is normal substantially, and clinical symptoms, sign are obviously improved;
Effectively: treated 7 days, body temperature descends, and clinical symptoms disappears substantially or cardinal symptom obviously alleviates; The auscultation of lung rale reduces, the X-ray Chest X-rays, and the lamellar shade dwindles;
Invalid: treated 7 days, body temperature does not fall or raises, and clinical symptoms is improved not obvious; Auscultation of lung and X-ray examination do not have significant change.
4 data processing methods
Measurement data adopts the t check, and ranked data adopt Ridit to analyze.
5 Therapeutic Method
Treatment group: adopt detoxifcation lung heat clearing drop pill.
Matched group: give the amantadine hydrochloride sheet, (the accurate word of traditional Chinese medicines: H21020714, manufacturer: Dongbei Pharmaceutical General Factory, main component are amantadine hydrochloride) 0.1g/ sheet, 1 0.3g, 1 day 3 times.
Annotate: treatment group, matched group body temperature suitably give Physical temperature-lowering 39.1～40 ℃ of persons.
6 therapeutic outcomes
Relatively there were significant differences for treatment group and matched group curative effect, and treatment group curative effect is better than matched group.See two groups of curative effect comparision tables 1.
Table 1 liang group Comparison of therapeutic table [n (%)]
Annotate: two groups of Comparison of therapeutic, analyze P＜0.01 through Ridit.
The result: 64 routine viral pneumonia patients use detoxifcation lung heat clearing prescription decoction treatment 7 days, cure 38 examples, produce effects 13 examples, and 11 examples that take a turn for the better, invalid 2 examples, total effective rate is 96.88%.
Conclusion: it is evident in efficacy that the present invention treats viral pneumonia.
Experimental example 2 effect experiments
1, to the cooling effect of fever in rabbit:
Get healthy rabbits, after surveying normal body temperature, iv Typhoid And Paratyphoid A, B Vaccine 0.5ml/kg, select 48 of 1 ℃ of persons of body temperature rise behind the 1h, be divided into 8 groups at random, respectively ig5g crude drug/kg, the normal saline of aspirin 0.1g/kg and equivalent, 2h, 4h respectively survey body temperature 1 time after the administration, and the variation of body temperature before and after the record administration the results are shown in Table:
Drop pill-1: Herba Ajugae 15g, Radix Scutellariae 10g, Radix Rhodiolae 5g; Method for making is with embodiment 2.
Drop pill-2: Herba Ajugae 15g, Rhizoma Polygoni Cuspidati 10g, Radix Rhodiolae 5g; Method for making is with embodiment 1
Drop pill-3: Herba Ajugae 15g, Radix Scutellariae 10g, Radix Rhodiolae 5g, Pulvis Fellis Suis 1g; Method for making is with embodiment 3.
The three with the artificial fever rabbit is had tangible analgesic cooling effect, effect can continue more than the 4h.There is not notable difference between the three.
2, the influence of xylol induced mice auricle edema
Remove 80 of kunming mices, male and female half and half, body weight 18-22g is divided into 8 groups at random.Matched group gavages normal saline.Successive administration 5d, 1h after the last administration, every mice left side ear is coated with the 0.05ml caused by dimethylbenzene xylene and swells, and auris dextra is made normal control, puts to death mice behind the 1h, and deducting auris dextra weight with left ear weight is the swelling degree, the antiinflammatory action of comparative drug
| Group | Dosage (g/kg) | The example number | Swelling rate (%) |
| Normal saline | 8 | 0.86±0.43 | |
| The Herba Ajugae drop pill | 0.02 | 8 | 0.73±0.16 |
| Sutellaria root pills | 0.02 | 8 | 0.71±0.21 |
| Giant knotweed pills | 0.02 | 8 | 0.69±0.20 |
| The Pulvis Fellis Suis drop pill | 0.02 | 8 | 0.70±0.18 |
| Drop pill-1 | 0.02 | 8 | 0.50±0.22 |
| Drop pill-2 | 0.02 | 8 | 0.51±0.31 |
| Drop pill-3 | 0.02 | 8 | 0.51±0.34 |
Drop pill-1 prescription: Herba Ajugae 15g, Radix Scutellariae 10g, Radix Rhodiolae 5g; Method for making is with embodiment 2.Drop pill-2 prescription: Herba Ajugae 15g, Rhizoma Polygoni Cuspidati 10g, Radix Rhodiolae 5g; Method for making is with embodiment 1
Drop pill-3 prescription: Herba Ajugae 15g, Radix Scutellariae 10g, Radix Rhodiolae 5g, Pulvis Fellis Suis 1g; Method for making is with embodiment 3.
Following embodiment all can realize the described effect of above-mentioned experimental example
The specific embodiment
Embodiment 1: drop pill
Herba Ajugae 1500 grams, Rhizoma Polygoni Cuspidati 1000 grams, Radix Rhodiolae 500 grams;
The Herba Ajugae method adopts 70% ethanol extraction twice, measures reflux, extract, 2 hours with 10 times for the first time, extracts 1.5h with 8 times of amounts for the second time; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution, supernatant and collect residue.Supernatant B crosses post: adopt the AB-8 resin, last sample (supernatant B) concentration 0.2g/ml, absorption flow velocity 2BV/h, wash with water earlier, volume is 3BV (column volume doubly), is 20% ethanol elution 5BV and 70% ethanol elution 5BV with eluant successively again, and elution flow rate is 3BV/h; Two-part eluent is merged with residue, reclaim ethanol, vacuum drying.
Rhizoma Polygoni Cuspidati adopts 70% ethanol extraction 3 times, and each 1.5 hours, 8 times of amounts; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution gets supernatant, and collects residue.Supernatant is crossed post: adopt the D-101 resin, last sample concentration 0.15g/ml, absorption flow velocity 2.4BV/h, wash with water earlier, volume is 2BV, reuse 20% pure eluting 5BV, last is 60% ethanol and 90% ethanol with eluant successively, and the eluant consumption is 5BV, and elution flow rate is 3BV/h.60% and 90% eluent is merged with residue, reclaim ethanol, vacuum drying.
The Radix Rhodiolae medical material adds the water of 8 times of medical material amounts, extracts 3 times, each 60min.Extracting solution merges, and filters, and the centrifugal 30min of sample solution gets supernatant.Adopt the AB-8 macroporous resin column, last sample concentration 0.2g/ml, absorption flow velocity 2.5BV/h, washing volume 3BV, to adopt 80% ethanol elution 5BV behind distilled water, the 10% ethanol elution 5BV, elution flow rate is 3BV/h.80% eluent is reclaimed ethanol; Eluent is dissolved in water, and adopts 10000 ultrafilter membrane ultrafiltration, the filtrate concentrate drying.
It is fine powder that above-mentioned exsiccant medicine is pulverized, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is more than the 1:1.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.The drop pill of preparation is 10000.Drop pill, every 30mg, each 1 bag, 1g/ bag (33), every day 3 times, buccal or warm water delivery service after meal.
Function, main syndrome: heat-clearing and toxic substances removing protects lung.Be mainly used in pyretic toxicity and accumulate the traditional Chinese medical science " wind-warm lung-heat disease " due to the lung; The viral lower respiratory infection that modern medicine is alleged belongs to this category, can be by this disease Coryza Treated by Syndrome Differentiation.
Embodiment 2: drop pill
Herba Ajugae 1500g, Radix Scutellariae 1000g, Radix Rhodiolae 500g;
The Herba Ajugae method adopts 70% ethanol extraction twice, measures reflux, extract, 2 hours with 10 times for the first time, extracts 1.5h with 8 times of amounts for the second time; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution, supernatant and collect residue.Supernatant B crosses post: adopt the AB-8 resin, last sample (supernatant B) concentration 0.2g/ml, absorption flow velocity 2BV/h, wash with water earlier, volume is 3BV (column volume doubly), is 20% ethanol elution 5BV and 70% ethanol elution 5BV with eluant successively again, and elution flow rate is 3BV/h; Two-part eluent is merged with residue, reclaim ethanol, vacuum drying.
Radix Scutellariae extracting in water 3 times adds 12 times of amounts of water at every turn, extracts 0.5 hour at every turn.The effective site process for purification is defined as: under 80 ℃, transfer pH to 1-2, be incubated 15 minutes, filter, precipitate adds 2 times of water and stirs, and regulates more than the pH to 7 with 40% sodium hydroxide solution, 95% the ethanol that adds equivalent stirs and makes dissolving, filters, filtrate is regulated pH to 1-2 with hydrochloric acid, 60 ℃ are incubated 15 minutes, leave standstill, and filter, precipitate water and Different concentrations of alcohol washing are to neutral, and drying under reduced pressure promptly.
The Radix Rhodiolae medical material adds the water of 8 times of medical material amounts, extracts 3 times, each 60min.Extracting solution merges, and filters, and the centrifugal 30min of sample solution gets supernatant.Adopt the AB-8 macroporous resin column, last sample concentration 0.2g/ml, absorption flow velocity 2.5BV/h, washing volume 3BV, to adopt 80% ethanol elution 5BV behind distilled water, the 10% ethanol elution 5BV, elution flow rate is 3BV/h.80% eluent is reclaimed ethanol; Eluent is dissolved in water, and adopts 10000 ultrafilter membrane ultrafiltration, the filtrate concentrate drying.
It is fine powder that above-mentioned exsiccant medicine is pulverized, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is more than the 1:1.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.The drop pill of preparation is 10000.Drop pill, every 30mg, each 1 bag, 1g/ bag (33), every day 3 times, buccal or warm water delivery service after meal.
Embodiment 3: drop pill
Herba Ajugae 1500g, Radix Scutellariae 1000g, Radix Rhodiolae 500g, Pulvis Fellis Suis 100g;
The Herba Ajugae method adopts 70% ethanol extraction twice, measures reflux, extract, 2 hours with 9 times for the first time, extracts 1.5h with 7 times of amounts for the second time; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution, supernatant and collect residue.Supernatant B crosses post: adopt the AB-8 resin, last sample (supernatant B) concentration 0.2g/ml, absorption flow velocity 2BV/h, wash with water earlier, volume is 3BV (column volume doubly), is 20% ethanol elution 5BV and 70% ethanol elution 5BV with eluant successively again, and elution flow rate is 3BV/h; Two-part eluent is merged with residue, reclaim ethanol, vacuum drying.
Radix Scutellariae extracting in water 3 times adds 12 times of amounts of water at every turn, extracts 0.5 hour at every turn.The effective site process for purification is defined as: under 80 ℃, transfer pH to 1-2, be incubated 15 minutes, filter, precipitate adds 2 times of water and stirs, and regulates more than the pH to 7 with 40% sodium hydroxide solution, 95% the ethanol that adds equivalent stirs and makes dissolving, filters, filtrate is regulated pH to 1-2 with hydrochloric acid, 60 ℃ are incubated 15 minutes, leave standstill, and filter, precipitate water and Different concentrations of alcohol washing are to neutral, and drying under reduced pressure promptly.
The Radix Rhodiolae medical material adds the water of 9 times of medical material amounts, extracts 3 times, each 60min.Extracting solution merges, and filters, and the centrifugal 30min of sample solution gets supernatant.Adopt the AB-8 macroporous resin column, last sample concentration 0.2g/ml, absorption flow velocity 2.5BV/h, washing volume 3BV, to adopt 80% ethanol elution 5BV behind distilled water, the 10% ethanol elution 5BV, elution flow rate is 3BV/h.80% eluent is reclaimed ethanol; Eluent is dissolved in water, and adopts 10000 ultrafilter membrane ultrafiltration, the filtrate concentrate drying.
Above-mentioned exsiccant medicine and Pulvis Fellis Suis pulverizing are fine powder, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is more than the 1:1.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.The drop pill of preparation is 10000.Drop pill, every 30mg, each 1 bag, 1g/ bag (33), every day 3 times, buccal or warm water delivery service after meal.
Embodiment 4: drop pill
Herba Ajugae 1500 grams, Rhizoma Polygoni Cuspidati 1000 grams, Radix Rhodiolae 500 grams, Pulvis Fellis Suis 100g;
The Herba Ajugae method adopts 70% ethanol extraction twice, measures reflux, extract, 2 hours with 10 times for the first time, extracts 1.5h with 8 times of amounts for the second time; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution, supernatant and collect residue.Supernatant B crosses post: adopt the AB-8 resin, last sample (supernatant B) concentration 0.2g/ml, absorption flow velocity 2BV/h, wash with water earlier, volume is 3BV (column volume doubly), is 20% ethanol elution 5BV and 70% ethanol elution 5BV with eluant successively again, and elution flow rate is 3BV/h; Two-part eluent is merged with residue, reclaim ethanol, vacuum drying.
Rhizoma Polygoni Cuspidati adopts 70% ethanol extraction 3 times, and each 1.5 hours, 8 times of amounts; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution gets supernatant, and collects residue.Supernatant is crossed post: adopt the D-101 resin, last sample concentration 0.15g/ml, absorption flow velocity 2.4BV/h, wash with water earlier, volume is 2BV, reuse 20% pure eluting 5BV, last is 60% ethanol and 90% ethanol with eluant successively, and the eluant consumption is 5BV, and elution flow rate is 3BV/h.60% and 90% eluent is merged with residue, reclaim ethanol, vacuum drying.
The Radix Rhodiolae medical material adds the water of 8 times of medical material amounts, extracts 3 times, each 60min.Extracting solution merges, and filters, and the centrifugal 30min of sample solution gets supernatant.Adopt the AB-8 macroporous resin column, last sample concentration 0.2g/ml, absorption flow velocity 2.5BV/h, washing volume 3BV, to adopt 80% ethanol elution 5BV behind distilled water, the 10% ethanol elution 5BV, elution flow rate is 3BV/h.80% eluent is reclaimed ethanol; Eluent is dissolved in water, and adopts 10000 ultrafilter membrane ultrafiltration, the filtrate concentrate drying.
Above-mentioned exsiccant medicine and Pulvis Fellis Suis pulverizing are fine powder, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is more than the 1:1.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.The drop pill of preparation is 10000.Drop pill, every 30mg, each 1 bag, 1g/ bag (33), every day 3 times, buccal or warm water delivery service after meal.
Embodiment 5: drop pill
Herba Ajugae 1800 grams, Rhizoma Polygoni Cuspidati 1000 grams, Radix Rhodiolae 600 grams;
The Herba Ajugae method adopts 70% ethanol extraction twice, measures reflux, extract, 2 hours with 10 times for the first time, extracts 1.5h with 8 times of amounts for the second time; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution, supernatant and collect residue.Supernatant B crosses post: adopt the AB-8 resin, last sample (supernatant B) concentration 0.2g/ml, absorption flow velocity 2BV/h, wash with water earlier, volume is 3BV (column volume doubly), is 20% ethanol elution 5BV and 70% ethanol elution 5BV with eluant successively again, and elution flow rate is 3BV/h; Two-part eluent is merged with residue, reclaim ethanol, vacuum drying.
Rhizoma Polygoni Cuspidati adopts 70% ethanol extraction 2 times, and each 1.5 hours, 8 times of amounts; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution gets supernatant, and collects residue.Supernatant is crossed post: adopt the D-101 resin, last sample concentration 0.16g/ml, absorption flow velocity 2.5BV/h, wash with water earlier, volume is 2BV, reuse 25% pure eluting 5BV, last is 60% ethanol and 90% ethanol with eluant successively, and the eluant consumption is 5BV, and elution flow rate is 3BV/h.60% and 90% eluent is merged with residue, reclaim ethanol, vacuum drying.
The Radix Rhodiolae medical material adds the water of 7 times of medical material amounts, extracts 2 times, each 80min.Extracting solution merges, and filters, and the centrifugal 30min of sample solution gets supernatant.Adopt the AB-8 macroporous resin column, last sample concentration 0.2g/ml, absorption flow velocity 2.5BV/h, washing volume 3BV, to adopt 80% ethanol elution 5BV behind distilled water, the 10% ethanol elution 5BV, elution flow rate is 3BV/h.80% eluent is reclaimed ethanol; Eluent is dissolved in water, and adopts 10000 ultrafilter membrane ultrafiltration, the filtrate concentrate drying.
It is fine powder that above-mentioned exsiccant medicine is pulverized, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is 1:2.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.The drop pill of preparation is 10000.Drop pill, every 30mg, each 1 bag, 1g/ bag (33), every day 3 times, buccal or warm water delivery service after meal.
Embodiment 6: capsule
Herba Ajugae 1900g, Radix Scutellariae 900g, Radix Rhodiolae 700g;
The Herba Ajugae method adopts 70% ethanol extraction twice, measures reflux, extract, 2 hours with 10 times for the first time, extracts 1.5h with 8 times of amounts for the second time; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution, supernatant and collect residue.Supernatant B crosses post: adopt the AB-8 resin, last sample (supernatant B) concentration 0.2g/ml, absorption flow velocity 2BV/h, wash with water earlier, volume is 3BV (column volume doubly), is 20% ethanol elution 5BV and 70% ethanol elution 5BV with eluant successively again, and elution flow rate is 3BV/h; Two-part eluent is merged with residue, reclaim ethanol, vacuum drying.
Radix Scutellariae extracting in water 3 times adds 12 times of amounts of water at every turn, extracts 0.5 hour at every turn.The effective site process for purification is defined as: under 80 ℃, transfer pH to 1-2, be incubated 15 minutes, filter, precipitate adds 2 times of water and stirs, and regulates more than the pH to 7 with 40% sodium hydroxide solution, 95% the ethanol that adds equivalent stirs and makes dissolving, filters, filtrate is regulated pH to 1-2 with hydrochloric acid, 60 ℃ are incubated 15 minutes, leave standstill, and filter, precipitate water and Different concentrations of alcohol washing are to neutral, and drying under reduced pressure promptly.
The Radix Rhodiolae medical material adds the water of 8 times of medical material amounts, extracts 3 times, each 60min.Extracting solution merges, and filters, and the centrifugal 30min of sample solution gets supernatant.Adopt the AB-8 macroporous resin column, last sample concentration 0.2g/ml, absorption flow velocity 2.5BV/h, washing volume 3BV, to adopt 80% ethanol elution 5BV behind distilled water, the 10% ethanol elution 5BV, elution flow rate is 3BV/h.80% eluent is reclaimed ethanol; Eluent is dissolved in water, and adopts 10000 ultrafilter membrane ultrafiltration, the filtrate concentrate drying.
It is fine powder that above-mentioned exsiccant medicine is pulverized, and mixing is made capsule through conventional technology.
Embodiment 7: tablet
Herba Ajugae 1000g, Radix Scutellariae 1500g, Radix Rhodiolae 500g, Pulvis Fellis Suis 300g;
The Herba Ajugae method adopts 70% ethanol extraction twice, measures reflux, extract, 2 hours with 9 times for the first time, extracts 1.5h with 7 times of amounts for the second time; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution, supernatant and collect residue.Supernatant B crosses post: adopt the AB-8 resin, last sample (supernatant B) concentration 0.2g/ml, absorption flow velocity 2BV/h, wash with water earlier, volume is 3BV (column volume doubly), is 20% ethanol elution 5BV and 70% ethanol elution 5BV with eluant successively again, and elution flow rate is 3BV/h; Two-part eluent is merged with residue, reclaim ethanol, vacuum drying.
Radix Scutellariae extracting in water 3 times adds 12 times of amounts of water at every turn, extracts 0.5 hour at every turn.The effective site process for purification is defined as: under 80 ℃, transfer pH to 1-2, be incubated 15 minutes, filter, precipitate adds 2 times of water and stirs, and regulates more than the pH to 7 with 40% sodium hydroxide solution, 95% the ethanol that adds equivalent stirs and makes dissolving, filters, filtrate is regulated pH to 1-2 with hydrochloric acid, 60 ℃ are incubated 15 minutes, leave standstill, and filter, precipitate water and Different concentrations of alcohol washing are to neutral, and drying under reduced pressure promptly.
The Radix Rhodiolae medical material adds the water of 9 times of medical material amounts, extracts 3 times, each 60min.Extracting solution merges, and filters, and the centrifugal 30min of sample solution gets supernatant.Adopt the AB-8 macroporous resin column, last sample concentration 0.2g/ml, absorption flow velocity 2.5BV/h, washing volume 3BV, to adopt 80% ethanol elution 5BV behind distilled water, the 10% ethanol elution 5BV, elution flow rate is 3BV/h.80% eluent is reclaimed ethanol; Eluent is dissolved in water, and adopts 10000 ultrafilter membrane ultrafiltration, the filtrate concentrate drying.
Above-mentioned exsiccant medicine and Pulvis Fellis Suis pulverizing are fine powder, and mixing is made tablet through conventional technology.
Embodiment 8: granule
Herba Ajugae 1900 grams, Rhizoma Polygoni Cuspidati 1200 grams, Radix Rhodiolae 400 grams, Pulvis Fellis Suis 200g;
The Herba Ajugae method adopts 70% ethanol extraction twice, measures reflux, extract, 2 hours with 10 times for the first time, extracts 1.5h with 8 times of amounts for the second time; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution, supernatant and collect residue.Supernatant B crosses post: adopt the AB-8 resin, last sample (supernatant B) concentration 0.2g/ml, absorption flow velocity 2BV/h, wash with water earlier, volume is 3BV (column volume doubly), is 20% ethanol elution 5BV and 70% ethanol elution 5BV with eluant successively again, and elution flow rate is 3BV/h; Two-part eluent is merged with residue, reclaim ethanol, vacuum drying.
Rhizoma Polygoni Cuspidati adopts 70% ethanol extraction 3 times, and each 1.5 hours, 8 times of amounts; Extracting solution filters, merge extracting solution, the centrifugal 30min of extracting solution gets supernatant, and collects residue.Supernatant is crossed post: adopt the D-101 resin, last sample concentration 0.15g/ml, absorption flow velocity 2.4BV/h, wash with water earlier, volume is 2BV, reuse 20% pure eluting 5BV, last is 60% ethanol and 90% ethanol with eluant successively, and the eluant consumption is 5BV, and elution flow rate is 3BV/h.60% and 90% eluent is merged with residue, reclaim ethanol, vacuum drying.
The Radix Rhodiolae medical material adds the water of 8 times of medical material amounts, extracts 3 times, each 60min.Extracting solution merges, and filters, and the centrifugal 30min of sample solution gets supernatant.Adopt the AB-8 macroporous resin column, last sample concentration 0.2g/ml, absorption flow velocity 2.5BV/h, washing volume 3BV, to adopt 80% ethanol elution 5BV behind distilled water, the 10% ethanol elution 5BV, elution flow rate is 3BV/h.80% eluent is reclaimed ethanol; Eluent is dissolved in water, and adopts 10000 ultrafilter membrane ultrafiltration, the filtrate concentrate drying.
Above-mentioned exsiccant medicine and Pulvis Fellis Suis pulverizing are fine powder, and mixing is made granule through conventional technology.
Embodiment 9 drop pill
Herba Ajugae 1500 grams, Rhizoma Polygoni Cuspidati 1000 grams, Radix Rhodiolae 500 grams;
Above-mentioned medical material adds 70% ethanol extraction 3 times, and each ethanol consumption is 6 times (weight ratios) of medical material amount, extracts 1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets extracting solution; Said extracted liquid is filtered, and filtrate is by macroporous resin, successively uses 5 times of water gagings, 20% ethanol elution, and eluent is abandoned or adopted, and reuse adds 5 times of eluting of 80% ethanol, collects eluent, and decompression recycling ethanol is not to there being the alcohol flavor, the purification extracting solution, drying.
It is fine powder that above-mentioned exsiccant medicine is pulverized, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is more than the 1:1.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.The drop pill of preparation is 10000.
Embodiment 10 capsules
Herba Ajugae 1500g, Radix Scutellariae 1000g, Radix Rhodiolae 500g;
Add 60% ethanol extraction 3 times, each ethanol consumption is 7 times (weight ratios) of medical material amount, extracts 2 hours at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets extracting solution, and drying is made capsule through conventional technology.
Embodiment 11: drop pill
The Herba Ajugae alcohol extraction crosses that refining thing 150 grams of post, Rhizoma Polygoni Cuspidati alcohol extraction are crossed refining thing 100 grams of post, refining thing 50 grams of post are crossed in the Radix Rhodiolae alcohol extraction;
It is fine powder that the said extracted thing is pulverized, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is 1:3.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.The drop pill of preparation.
Embodiment 12: drop pill
Post refining thing 110g, Radix Rhodiolae ethanol extract 60g are crossed in Herba Ajugae ethanol extract 160g, Radix Scutellariae alcohol extraction
It is fine powder that the said extracted thing is pulverized, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is 1:2.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.The drop pill of preparation.Embodiment 13: drop pill
Herba Ajugae water was carried the refining thing 200g of post, Radix Scutellariae water was carried the refining thing 150g of post, Radix Rhodiolae ethanol extract 50g, Pulvis Fellis Suis 100g;
It is fine powder that the said extracted thing is pulverized, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is 1:1.5.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.The drop pill of preparation.
Embodiment 14: drop pill
Herba Ajugae extract 250g, Rhizoma Polygoni Cuspidati extract 200g, Radix Rhodiolae extract 120g, Pulvis Fellis Suis 200g;
It is fine powder that the said extracted thing is pulverized, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is 1:3.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.The drop pill of preparation.
Embodiment 15: drop pill
Herba Ajugae extract 250g, Rhizoma Polygoni Cuspidati extract 200g, Radix Rhodiolae extract 120g, Pulvis Fellis Suis 70g;
It is fine powder that the said extracted thing is pulverized, and mixing joins among the molten matrix PEG4000 (70 ℃ of melt temperatures), and the ratio of its Chinese medicine and substrate is 1:3.Condensing agent is a methyl-silicone oil-50; Drip 70～85 ℃ of system temperature, drip, cooling column length 50cm, condensation temperature: ice-water bath apart from 5cm.The drop pill of preparation.
Claims (10)
1. Chinese medicine composition for the treatment of viral lower respiratory infection is characterized in that being made by the crude drug of following weight ratio:
Herba Ajugae 10-20 weight portion, Rhizoma Polygoni Cuspidati 8-15 weight portion, Radix Rhodiolae 3-8 weight portion.
2. Chinese medicine composition as claimed in claim 1 is characterized in that being made by the crude drug of following weight ratio:
Herba Ajugae 15 weight portions, Rhizoma Polygoni Cuspidati 10 weight portions, Radix Rhodiolae 5 weight portions.
3. Chinese medicine composition for the treatment of viral lower respiratory infection is characterized in that being made by the crude drug of following weight ratio:
Herba Ajugae 10-20 weight portion, Radix Scutellariae 8-15 weight portion, Radix Rhodiolae 3-8 weight portion.
4. Chinese medicine composition as claimed in claim 3 is characterized in that being made by the crude drug of following weight ratio:
Herba Ajugae 15 weight portions, Radix Scutellariae 10 weight portions, Radix Rhodiolae 5 weight portions.
5. a Chinese medicine composition for the treatment of viral lower respiratory infection is characterized in that adding Pulvis Fellis Suis 0.1-1 weight portion and makes on the basis of one of claim 1-4 described crude drug.
6. Chinese medicine composition for the treatment of viral lower respiratory infection is characterized in that being made by the crude drug of following weight ratio:
Herba Ajugae extract 10-20 weight portion, Rhizoma Polygoni Cuspidati extract 8-15 weight portion, Radix Rhodiolae extract 3-8 weight portion.
7. Chinese medicine composition for the treatment of viral lower respiratory infection is characterized in that being made by the crude drug of following weight ratio:
Herba Ajugae extract 10-20 weight portion, Radix Scutellariae extract 8-15 weight portion, Radix Rhodiolae extract 3-8 weight portion.
8. a Chinese medicine composition for the treatment of viral lower respiratory infection is characterized in that adding Pulvis Fellis Suis 2.5-25 weight portion and makes on the basis of claim 6 or 7 described crude drug.
9. Chinese medicine composition as claimed in claim 6 is characterized in that:
Total phenolic acid, content of total flavone are more than 25% in the Herba Ajugae extract;
The total anthraquinones content sum is more than 15% in the Rhizoma Polygoni Cuspidati extract; General flavone content is more than 20% in the Radix Rhodiolae extract.
10. Chinese medicine composition as claimed in claim 7 is characterized in that:
Total phenolic acid, content of total flavone are more than 25% in the Herba Ajugae extract; General flavone content is more than 20% in the Radix Rhodiolae extract.
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| CN102349947A (en) * | 2011-10-31 | 2012-02-15 | 陕西康惠制药股份有限公司 | Traditional Chinese medicine composition for treating viral lower respiratory tract infection |
| CN102362890B (en) * | 2011-11-28 | 2013-09-18 | 陕西康惠制药股份有限公司 | Chinese medicinal composition for treating viral pneumonia |
| WO2013173960A1 (en) * | 2012-05-21 | 2013-11-28 | 深圳市齐旺投资有限公司 | Traditional chinese medicine composition for treating lower respiratory tract viral infectious diseases and preparation method therefor |
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