CN101326960B - Use of radioresistant cocci in preparing feedstuff additive for improving fertility of sow - Google Patents
Use of radioresistant cocci in preparing feedstuff additive for improving fertility of sow Download PDFInfo
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- CN101326960B CN101326960B CN2008100630994A CN200810063099A CN101326960B CN 101326960 B CN101326960 B CN 101326960B CN 2008100630994 A CN2008100630994 A CN 2008100630994A CN 200810063099 A CN200810063099 A CN 200810063099A CN 101326960 B CN101326960 B CN 101326960B
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Abstract
The invention provides the application of Deinococcus radiodurans in preparing feed additive which improves the reproductive capability of sows, and the feed additive prepared by the Deinococcus radiodurans. The feed additive of the invention has been proved after a plurality of times of feeding tests and lab tests that: the feed additive has stable product characters, and safe and high-efficiency characteristics; 0.1 percent to 0.5 percent of the feed additive of the invention is added based on sow feed to feed, which can obviously improve the number born alive, milk yield in lactation, weaning litter weight and post-partum estrus rate of the sows, has obvious economic and social benefit, and broad market prospect.
Description
(1) technical field
The present invention relates to the application of radioresistant cocci in the feed addictive of preparation raising fertility of sow, and a kind of feed addictive that improves fertility of sow.
(2) background technology
Fertility of sow is one of key factor of decision pig farm economic benefit, and how improving fertility of sow is the focus of current livestock technology worker research.The index of fertility of sow has nest litter size, annual output parity and the weight of weaning litter etc. of sow.The number of eggs ovulated that sow is 1 time is about 20～30, and average nest litter size is about 11～12, accounts for 50% of sow productive potentialities, and all the other 50% but fail to bring into play.Therefore, how to promote sow to oestrus normally and ovulate, reduce embryonic death, preventing tire, stillborn foetus, miscarriage are the effective ways of reproductive capacity that improves sow.In addition, promote to oestrus as early as possible after the sow wean, shorten the time interval that sow is being planted from weaning to mixing, increase the annual output parity of sow, also have positive role improving fertility of sow.
Some studies show that vitamin has the reproductive capacity that improves sow.For example folic acid plays an important role to keeping sow reproductive status and early embryonic development, adds folic acid and can improve embryo survival, and additional most critical period is pregnant 60d.Matte etc. think that injection folic acid can improve sow reproductive performance during sow gestation.Reports such as Tremblay add survival rate and protein concentration that folic acid can increase pregnant 34d sow fetus, and when ovulation rate was high, the effect that folic acid reduces rate of embryonic death was more obvious.And for example biotin can shorten inter oestrual period, improves the nest litter size of multiparity sow, promotes gestational period metreurysma and placentation, increases cornua uteri length and surface area of placenta, better for fetus provides nutrition, has promoted reaching full growth of fetus.Biotin participates in energetic supersession, and can stimulate estrogenic secretion, reduces rate out of heat, can reduce the incidence of disease of drylot feeding sow pig and brood sow limb hoof disease.
(3) summary of the invention
The invention provides the application of radioresistant cocci (Deinococcus radiodurans) in the feed addictive of preparation raising fertility of sow, a kind of feed addictive that utilizes radioresistant cocci to improve fertility of sow is provided simultaneously.
The technical solution used in the present invention is as follows:
The application of radioresistant cocci (Deinococcus radiodurans) in the feed addictive of preparation raising fertility of sow.
Preferably, described radioresistant cocci is wild-type strain D.radiodurans R1.
The invention provides the feed addictive that improves fertility of sow, contain radioresistant cocci (Deinococcus radiodurans) during the feed of described raising fertility of sow adds through break process.
Concrete, described feed addictive by radioresistant cocci after break process, with quality for through the folic acid of 1～250 times of the radioresistant cocci of break process or composite the making of mixture of biotin or folic acid and biotin.
Described radioresistant cocci break process method can be undertaken by this area conventional method, and used breaking method is as follows among the present invention:
(1) with the solid medium (as TGY solid medium) of radioresistant cocci bacterial classification inoculation to suitable radioresistant cocci, cultivate 32～36h for 30～32 ℃, picking list colony inoculation is to the fluid nutrient medium (as the TGY fluid nutrient medium) that is fit to radioresistant cocci, and 30～32 ℃ are cultured to exponential phase;
(2) step (1) is obtained liquid seeds by volume 5.1%～6.0% inoculum concentration be seeded to the fermentation medium that is fit to radioresistant cocci, cultivate 45～48h under ℃ condition of pH6.0～8.0,30～32; The culture medium that is fit to has: (consist of in every 1000ml culture medium: fineness is 80 purpose soy bean powder 30g to the expanded soybean culture medium, glucose 2g, sodium nitrate 2g, adding distil water is to 1000mL), the TGY fluid nutrient medium, (consist of in every 1000ml culture medium: fineness is 80 purpose corn flour 30g to maize powder medium, glucose 1.5g, sodium nitrate 2.5g, adding distil water is to 1000mL) etc., preferred expanded soybean culture medium.
(3) get the zymotic fluid that step (2) obtains, centrifugal, the radioresistant cocci wet thallus is tiled into 0.1cm～1.0cm thickness, under frequency 600～980MHz, electric-field intensity 8000～9500v/m condition, handle 30～50s, cold air drying obtains the radioresistant cocci through break process to water content below 6% under 5～35 ℃, wind speed 2～3m/s, relative humidity 10～30% conditions.
Preferably, described feed addictive is made up of the radioresistant cocci and the folic acid through break process of mass ratio 1: 1～5.
Preferably, described feed addictive is made up of the radioresistant cocci and the biotin through break process of mass ratio 1: 10～30.Preferred, described feed addictive is made up of the radioresistant cocci and the biotin through break process of mass ratio 1: 20～24.
Preferably, described feed addictive is made up of the radioresistant cocci through break process, folic acid and the biotin of mass ratio 1: 30～50: 100～200.Preferred, described feed addictive is made up of the radioresistant cocci through break process, folic acid and the biotin of mass ratio 1: 30～35: 100～110.
In the described feed addictive, used radioresistant cocci is preferably wild-type strain D.radiodurans R1.
Feed addictive of the present invention addition in the daily feed of sow is 0.1～0.5% (w/w).
Because it is limited that single vitamin improves the effect of fertility of sow,, carried out groping in a large number screening test for development of new improves the feed addictive of fertility of sow safely and efficiently.The results showed, add the treated radioresistant cocci that is rich in lycopene, beta carotene, gamma carotene and lutein at the sow daily ration and can be able to obviously improve sow and produce the young number of living, lactation lactation amount, weight of weaning litter and post-partum estrus rate.The present invention on this basis, carried out radioresistant cocci and folic acid, biotin has carried out combined effect research, verify these three kinds of materials and had tangible combination synergy, provide a kind of new type of safe to utilize radioresistant cocci to improve the feed addictive of fertility of sow efficiently.
Feed addictive of the present invention detects through repeatedly feeding experiment and laboratory and shows: the product proterties is stable, has safe and efficient characteristic, on the basis of sow mixed feed, add 0.1～0.5% feed addictive of the present invention and feed, can obviously improve sow produce to live young number (improving 10.51%～18.54%), improve the lactation lactation amount and make weight of weaning litter (improving 15.14%～21.07%) and the ablactation rate (raising 4.54%～12.50%) of oestrusing in back 7 days.Additive of the present invention has remarkable economic efficiency and social benefit, and market prospects are wide.
(4) specific embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
1, the radioresistant cocci through break process prepares
The strain classification of used radioresistant cocci is Deinococcus radiodurans, is provided by U.S. typical case DSMZ, and preserving number is ATCC NO.13939, and bacterium numbering is R1, and concrete steps are:
(1) activation of bacterial classification, go down to posterity: the radioresistant cocci streak inoculation on the TGY solid medium, is cultivated 32h for 30 ℃, and picking list colony inoculation is cultured to exponential phase in triangular flask TGY fluid nutrient medium; The preparation of TGY fluid nutrient medium: consist of peptone 10g in every 1000ml culture medium, glucose 1g, yeast extract 5g, adding distil water is to 1000ml; The preparation of TGY solid medium consists of in every 1000ml culture medium: peptone 10g, and glucose 1g, yeast extract 5g, agar 15g, adding distil water is to 1000ml;
(2) fermented and cultured: with the liquid seeds of activation in (1) is that 5.1% inoculum concentration inserts the culture medium that is mixed with expanded soybean and carries out fermented and cultured by volume, and pH6.0 cultivates 45h for 30 ℃; The expanded soybean culture medium preparation consists of in every 1000ml culture medium: fineness is 80 purpose soy bean powder 30g, glucose 2g, and sodium nitrate 2g, adding distil water is to 1000mL.
(3) take out cultured thalline zymotic fluid in (2), in the centrifugal 5min of 5000rpm, get the radioresistant cocci wet thallus, it is paved into the thickness of 0.1cm equably, at frequency 600MHZ, handled 30 seconds under the condition of electric-field intensity 8000v/m, make clasmatosis, treated radioresistant cocci is placed material disc, put into and carry out cold air drying in the cold wind drier, chilling temperature is controlled at 25 ℃, wind speed 2 meter per seconds, relative humidity 10% is dried to water content below 6%, obtains the radioresistant cocci through fragmentation.
2, compatibility combination
To make up in 1: 1 ratio of mass ratio through radioresistant cocci and two kinds of materials of folic acid of fragmentation, and above-mentioned compound is mixed, obtain a kind of feed addictive that improves fertility of sow.
3, effect
Control group adopts basal diet, and test group is added the feed addictive that 0.1% (adding the 0.1kg additive in every 100kg basal diet) pressed the raising fertility of sow of present embodiment method production in basal diet.Experimental period, begin to begin to the gestation of following one-period from nonpregnant sows, totally 40 of test sows, and every group each 20, kind is that big * is long, the parity unanimity.Effect sees Table 1.
Table 1: feed addictive of the present invention is to the influence of fertility of sow
| Produce the young number of living | Weight of weaning litter (Kg) | The rate % that oestruses in back 7 days weans | |
| Control group | 10.35±1.69A | 62.56±8.98A | 90 |
| Test group | 11.70±1.34B | 74.34±8.54B | 95 |
Annotate: annotate different capitalizations with delegation's acceptance of the bid and represent difference extremely significantly (P＜0.01).
Embodiment 2:
1, the radioresistant cocci through fragmentation prepares
The strain classification of used radioresistant cocci is Deinococcus radiodurans, is provided by U.S. typical case DSMZ, and preserving number is ATCC NO.13939, and bacterium numbering is R1, and concrete steps are:
(1) activation of bacterial classification, go down to posterity: the radioresistant cocci streak inoculation on the TGY solid medium, is cultivated 34h for 31 ℃, and picking list colony inoculation is cultured to exponential phase in triangular flask TGY fluid nutrient medium;
(2) fermented and cultured: with the liquid seeds of activation in (2) is that 5.5% inoculum concentration inserts the culture medium that is mixed with expanded soybean and carries out fermented and cultured by volume, and pH7.0 cultivates 46.5h for 31 ℃; The expanded soybean culture medium preparation, every 1000mL expanded soybean culture medium: fineness is 80 purpose soy bean powder 30g, glucose 2g, sodium nitrate 2g, adding distil water is to 1000mL.
(3) take out cultured thalline zymotic fluid in (2), in the centrifugal 7.5min of 6500rpm, get the radioresistant cocci wet thallus, it is paved into the thickness of 0.55cm equably, at frequency 790MHZ, handled 40 seconds under the condition of electric-field intensity 8750v/m, make clasmatosis, treated radioresistant cocci is placed material disc, put into and carry out cold air drying in the cold wind drier, chilling temperature is controlled at 30 ℃, wind speed 2.5 meter per seconds, relative humidity 20% is dried to water content below 6%, obtains the radioresistant cocci through fragmentation.
2, compatibility combination
To make up in 1: 3 ratio of mass ratio through radioresistant cocci and two kinds of materials of folic acid of fragmentation, and above-mentioned compound is mixed, obtain a kind of feed addictive that improves fertility of sow.
3, effect
Control group adopts basal diet, and test group is added the feed addictive that 0.5% (adding the 0.5kg additive in every 100kg basal diet) pressed the raising fertility of sow of present embodiment method production in basal diet.Experimental period, begin to begin to the gestation of following one-period from nonpregnant sows, totally 86 of test sows, and every group each 43, kind is that big * is long, the parity unanimity.Effect sees Table 2.
Table 2: feed addictive of the present invention is to the influence of fertility of sow
| Produce the young number of living | Weight of weaning litter (Kg) | The rate % that oestruses in back 7 days weans | |
| Control group | 10.42±1.28A | 61.56±7.39A | 90.69 |
| Test group | 11.69±1.53B | 70.88±8.48B | 95.35 |
Annotate: annotate different capitalizations with delegation's acceptance of the bid and represent difference extremely significantly (P＜0.01).
Embodiment 3:
1, the radioresistant cocci through fragmentation prepares
The strain classification of used radioresistant cocci is Deinococcus radiodurans, is provided by U.S. typical case DSMZ, and preserving number is ATCC NO.13939, and bacterium numbering is R1, and concrete steps are:
(1) activation of bacterial classification, go down to posterity: the radioresistant cocci streak inoculation on the TGY solid medium, is cultivated 36h for 32 ℃, and picking list colony inoculation is cultured to exponential phase in triangular flask TGY fluid nutrient medium;
(2) fermented and cultured: with the liquid seeds of activation in (1) is that 6% inoculum concentration inserts the culture medium that is mixed with expanded soybean and carries out fermented and cultured by volume, and pH8.0 cultivates 48h for 32 ℃; The expanded soybean culture medium preparation, every 1000mL expanded soybean culture medium: fineness is 80 purpose soy bean powder 30g, glucose 2g, sodium nitrate 2g, adding distil water is to 1000mL.
(3) take out cultured thalline zymotic fluid in (2),, get the radioresistant cocci wet thallus in the centrifugal 10min of 8000rpm, it is paved into the thickness of 1cm equably,, handled 50 seconds under the condition of electric-field intensity 9500v/m at frequency 980MHZ, make clasmatosis, treated radioresistant cocci is placed material disc, put into and carry out cold air drying in the cold wind drier, chilling temperature is controlled at 35 ℃, wind speed 3 meter per seconds, relative humidity 30% is dried to water content below 6%, obtains the radioresistant cocci through fragmentation.
2, compatibility combination
To make up in 1: 5 ratio of mass ratio through radioresistant cocci and two kinds of materials of folic acid of fragmentation, and above-mentioned compound is mixed, obtain a kind of feed addictive that improves fertility of sow.
3, effect
Control group adopts basal diet, and test group is added the feed addictive that 0.25% (adding the 0.25kg additive in every 100kg basal diet) pressed the raising fertility of sow of present embodiment method production in basal diet.Experimental period, begin to begin to the gestation of following one-period from nonpregnant sows, totally 78 of test sows, and every group each 39, kind is that big * is long, the parity unanimity.Effect sees Table 3.
Table 3: feed addictive of the present invention is to the influence of fertility of sow
| Produce the young number of living | Weight of weaning litter (Kg) | The rate % that oestruses in back 7 days weans | |
| Control group | 10.15±1.43A | 62.18±8.13A | 89.74 |
| Test group | 11.53±1.23B | 72.33±7.59B | 97.44 |
Annotate: annotate different capitalizations with delegation's acceptance of the bid and represent difference extremely significantly (P＜0.01).
Embodiment 4:
1, the radioresistant cocci through fragmentation prepares
The strain classification of used radioresistant cocci is Deinococcus radiodurans, is provided by U.S. typical case DSMZ, and preserving number is ATCC NO.13939, and bacterium numbering is R1, and concrete steps are:
(1) activation of bacterial classification, go down to posterity: the radioresistant cocci streak inoculation on the TGY solid medium, is cultivated 32h for 30 ℃, and picking list colony inoculation is cultured to exponential phase in triangular flask TGY fluid nutrient medium;
(2) fermented and cultured: with the liquid seeds of activation in (1) is that 5.1% inoculum concentration inserts the culture medium that is mixed with expanded soybean and carries out fermented and cultured by volume, and pH6.0 cultivates 45h for 30 ℃; The expanded soybean culture medium preparation, every 1000mL soya broth: fineness is 80 purpose soy bean powder 30g, glucose 2g, sodium nitrate 2g, adding distil water is to 1000mL.
(3) take out cultured thalline zymotic fluid in (2), in the centrifugal 5min of 5000rpm, get the radioresistant cocci wet thallus, it is paved into the thickness of 0.1cm equably, at frequency 600MHZ, handled 30 seconds under the condition of electric-field intensity 8000v/m, make clasmatosis, treated radioresistant cocci is placed material disc, put into and carry out cold air drying in the cold wind drier, chilling temperature is controlled at 25 ℃, wind speed 2 meter per seconds, relative humidity 10% is dried to water content below 6%, obtains the radioresistant cocci through fragmentation.
2, compatibility combination
Radioresistant cocci and two kinds of materials of biotin of fragmentation are made up in 1: 10 ratio of mass ratio, and above-mentioned compound is mixed, obtain a kind of feed addictive that improves fertility of sow.
3, effect
Control group adopts basal diet, and test group is added the feed addictive that 0.3% (adding the 0.3kg additive in every 100kg basal diet) pressed the raising fertility of sow of present embodiment method production in basal diet.Experimental period, begin to begin to the gestation of following one-period from nonpregnant sows, totally 56 of test sows, and every group each 28, kind is that big * is long, the parity unanimity.Effect sees Table 4.
Table 4: feed addictive of the present invention is to the influence of fertility of sow
| Produce the young number of living | Weight of weaning litter (Kg) | The rate % that oestruses in back 7 days weans | |
| Control group | 10.32±1.58A | 67.51±8.11A | 85.71 |
| Test group | 11.75±1.40B | 79.86±9.45B | 96.43 |
Annotate: annotate different capitalizations with delegation's acceptance of the bid and represent difference extremely significantly (P＜0.01).
Embodiment 5:
1, the radioresistant cocci through fragmentation prepares
The strain classification of used radioresistant cocci is Deinococcus radiodurans, is provided by U.S. typical case DSMZ, and preserving number is ATCC NO.13939, and bacterium numbering is R1, and concrete steps are:
(1) activation of bacterial classification, go down to posterity: the radioresistant cocci streak inoculation on the TGY solid medium, is cultivated 34h for 31 ℃, and picking list colony inoculation is cultured to exponential phase in triangular flask TGY fluid nutrient medium;
(2) fermented and cultured: with the liquid seeds of activation in (1) is that 5.5% inoculum concentration inserts the culture medium that is mixed with expanded soybean and carries out fermented and cultured by volume, and pH7.0 cultivates 46.5h for 31 ℃; The expanded soybean culture medium preparation, every 1000mL expanded soybean culture medium: fineness is 80 purpose soy bean powder 30g, glucose 2g, sodium nitrate 2g, adding distil water is to 1000mL.
(3) take out cultured thalline zymotic fluid in (2), in the centrifugal 7.5min of 6500rpm, get the radioresistant cocci wet thallus, it is paved into the thickness of 0.55cm equably, at frequency 790MHZ, handled 40 seconds under the condition of electric-field intensity 8750v/m, make clasmatosis, treated radioresistant cocci is placed material disc, put into and carry out cold air drying in the cold wind drier, chilling temperature is controlled at 30 ℃, wind speed 2.5 meter per seconds, relative humidity 20% is dried to water content below 6%, obtains the radioresistant cocci through fragmentation.
2, compatibility combination
Radioresistant cocci and two kinds of materials of biotin of fragmentation are made up in 1: 20 ratio of mass ratio, and above-mentioned compound is mixed, obtain a kind of feed addictive that improves fertility of sow.
3, effect
Control group adopts basal diet, and test group is added the feed addictive that 0.2% (adding the 0.2kg additive in every 100kg basal diet) pressed the raising fertility of sow of present embodiment method production in basal diet.Experimental period, begin to begin to the gestation of following one-period from nonpregnant sows, totally 110 of test sows, and every group each 55, kind is that big * is long, the parity unanimity.Effect sees Table 5.
Table 5: feed addictive of the present invention is to the influence of fertility of sow
| Produce the young number of living | Weight of weaning litter (Kg) | The rate % that oestruses in back 7 days weans | |
| Control group | 10.47±1.57A | 64.87±9.75A | 89.09 |
| Test group | 11.57±1.43B | 78.39±9.56B | 96.36 |
Annotate: annotate different capitalizations with delegation's acceptance of the bid and represent difference extremely significantly (P＜0.01).
Embodiment 6:
1, the radioresistant cocci through fragmentation prepares
The strain classification of used radioresistant cocci is Deinococcus radiodurans, is provided by U.S. typical case DSMZ, and preserving number is ATCC NO.13939, and bacterium numbering is R1, and concrete steps are:
(1) activation of bacterial classification, go down to posterity: the radioresistant cocci streak inoculation on the TGY solid medium, is cultivated 36h for 32 ℃, and picking list colony inoculation is cultured to exponential phase in triangular flask TGY fluid nutrient medium;
(2) fermented and cultured: with the liquid seeds of activation in (1) is that 6% inoculum concentration inserts the culture medium that is mixed with expanded soybean and carries out fermented and cultured by volume, and pH8.0 cultivates 48h for 32 ℃; The expanded soybean culture medium preparation, every 1000mL expanded soybean culture medium: fineness is 80 purpose soy bean powder 30g, glucose 2g, sodium nitrate 2g, adding distil water is to 1000mL.
(3) take out cultured thalline zymotic fluid in (2),, get the radioresistant cocci wet thallus in the centrifugal 10min of 8000rpm, it is paved into the thickness of 1cm equably,, handled 50 seconds under the condition of electric-field intensity 9500v/m at frequency 980MHZ, make clasmatosis, treated radioresistant cocci is placed material disc, put into and carry out cold air drying in the cold wind drier, chilling temperature is controlled at 35 ℃, wind speed 3 meter per seconds, relative humidity 30% is dried to water content below 6%, obtains the radioresistant cocci through fragmentation.
2, compatibility combination
Radioresistant cocci and two kinds of materials of biotin of fragmentation are made up in 1: 30 ratio of mass ratio, and above-mentioned compound is mixed, obtain a kind of feed addictive that improves fertility of sow.
3, effect
Control group adopts basal diet, and test group is added the feed addictive that 0.4% (adding the 0.4kg additive in every 100kg basal diet) pressed the raising fertility of sow of present embodiment method production in basal diet.Experimental period, begin to begin to the gestation of following one-period from nonpregnant sows, totally 38 of test sows, and every group each 19, kind is that big * is long, the parity unanimity.Effect sees Table 6.
Table 6: feed addictive of the present invention is to the influence of fertility of sow
| Produce the young number of living | Weight of weaning litter (Kg) | The rate % that oestruses in back 7 days weans | |
| Control group | 10.42±1.61A | 67.13±10.37A | 84.21 |
| Test group | 12.16±1.07B | 80.75±7.09B | 94.74 |
Annotate: annotate different capitalizations with delegation's acceptance of the bid and represent difference extremely significantly (P＜0.01).
Embodiment 7:
1, the radioresistant cocci through fragmentation prepares
The strain classification of used radioresistant cocci is Deinococcus radiodurans, is provided by U.S. typical case DSMZ, and preserving number is ATCC NO.13939, and bacterium numbering is R1, and concrete steps are:
(1) activation of bacterial classification, go down to posterity: on radioresistant cocci streak inoculation TGY solid medium, cultivate 32h for 30 ℃, picking list colony inoculation is cultured to exponential phase in triangular flask TGY fluid nutrient medium;
(2) fermented and cultured: with the liquid seeds of activation in (1) is that 5.1% inoculum concentration inserts the culture medium that is mixed with expanded soybean and carries out fermented and cultured by volume, and pH6.0 cultivates 45h for 30 ℃; The expanded soybean culture medium preparation, every 1000mL expanded soybean culture medium: fineness is 80 purpose soy bean powder 30g, glucose 2g, sodium nitrate 2g, adding distil water 1000mL.
(3) take out cultured thalline zymotic fluid in (2), in the centrifugal 5min of 5000rpm, get the radioresistant cocci wet thallus, it is paved into the thickness of 0.1cm equably, at frequency 600MHZ, handled 30 seconds under the condition of electric-field intensity 8000v/m, make clasmatosis, treated radioresistant cocci is placed material disc, put into and carry out cold air drying in the cold wind drier, chilling temperature is controlled at 25 ℃, wind speed 2 meter per seconds, relative humidity 10% is dried to water content below 6%, obtains the radioresistant cocci through fragmentation.
2, compatibility combination
Will be through the radioresistant cocci of fragmentation and folic acid and three kinds of materials of biotin in mass ratio ratio combination in 1: 30: 100, and above-mentioned compound mixed, a kind of feed addictive that improves fertility of sow obtained.
3, effect
Control group adopts basal diet, and test group is added the feed addictive that 0.1% (adding the 0.1kg additive in every 100kg basal diet) pressed the raising fertility of sow of present embodiment method production in basal diet.Experimental period, begin to begin to the gestation of following one-period from nonpregnant sows, totally 260 of test sows, and every group each 130, kind is that big * is long, the parity unanimity.Effect sees Table 7.
Table 7: feed addictive of the present invention is to the influence of fertility of sow
| Produce the young number of living | Weight of weaning litter (Kg) | The rate % that oestruses in back 7 days weans | |
| Control group | 10.30±1.48A | 64.18±7.69A | 89.23 |
| Test group | 12.21±0.95B | 75.63±9.27B | 96.15 |
Annotate: annotate different capitalizations with delegation's acceptance of the bid and represent difference extremely significantly (P＜0.01).
Embodiment 8:
1, the radioresistant cocci through fragmentation prepares
The strain classification of used radioresistant cocci is Deinococcus radiodurans, is provided by U.S. typical case DSMZ, and preserving number is ATCC NO.13939, and bacterium numbering is R1, and concrete steps are:
(1) activation of bacterial classification, go down to posterity: the radioresistant cocci streak inoculation on the TGY solid medium, is cultivated 34h for 31 ℃, and picking list colony inoculation is cultured to exponential phase in triangular flask TGY fluid nutrient medium;
(2) fermented and cultured: with the liquid seeds of activation in (1) is that 5.5% inoculum concentration inserts the culture medium that is mixed with expanded soybean and carries out fermented and cultured by volume, and pH7.0 cultivates 46.5h for 31 ℃; The expanded soybean culture medium preparation, every 1000mL expanded soybean culture medium: fineness is 80 purpose soy bean powder 30g, glucose 2g, sodium nitrate 2g, adding distil water is to 1000mL.
(3) take out cultured thalline zymotic fluid in (2), in the centrifugal 7.5min of 6500rpm, get the radioresistant cocci wet thallus, it is paved into the thickness of 0.55cm equably, at frequency 790MHZ, handled 40 seconds under the condition of electric-field intensity 8750v/m, make clasmatosis, treated radioresistant cocci is placed material disc, put into and carry out cold air drying in the cold wind drier, chilling temperature is controlled at 30 ℃, wind speed 2.5 meter per seconds, relative humidity 20% is dried to water content below 6%, obtains the radioresistant cocci through fragmentation.
2, compatibility combination
Will be through the radioresistant cocci of fragmentation and folic acid and three kinds of materials of biotin in mass ratio ratio combination in 1: 40: 150, and above-mentioned compound mixed, a kind of feed addictive that improves fertility of sow obtained.
3, effect
Control group adopts basal diet, and test group is added the feed addictive that 0.1% (adding the 0.1kg additive in every 100kg basal diet) pressed the raising fertility of sow of present embodiment method production in basal diet.Experimental period, begin to begin to the gestation of following one-period from nonpregnant sows, totally 48 of test sows, and every group each 24, kind is that big * is long, the parity unanimity.Effect sees Table 8.
Table 8: feed addictive of the present invention is to the influence of fertility of sow
| Produce the young number of living | Weight of weaning litter (Kg) | The rate % that oestruses in back 7 days weans | |
| Control group | 10.63±1.61A | 65.13±10.15A | 91.67 |
| Test group | 11.88±1.30B | 78.85±8.61B | 95.83 |
Annotate: annotate different capitalizations with delegation's acceptance of the bid and represent difference extremely significantly (P＜0.01).
Embodiment 9:
1, the radioresistant cocci through fragmentation prepares
The strain classification of used radioresistant cocci is Deinococcus radiodurans, is provided by U.S. typical case DSMZ, and preserving number is ATCC NO.13939, and bacterium numbering is R1, and concrete steps are:
(1) activation of bacterial classification, go down to posterity: the radioresistant cocci streak inoculation on the TGY solid medium, is cultivated 36h for 32 ℃, and picking list colony inoculation is cultured to exponential phase in triangular flask TGY fluid nutrient medium;
(2) fermented and cultured: with the liquid seeds of activation in (1) is that 6% inoculum concentration inserts the culture medium that is mixed with expanded soybean and carries out fermented and cultured by volume, and pH8.0 cultivates 48h for 32 ℃; The expanded soybean culture medium preparation, every 1000mL expanded soybean culture medium: fineness is 80 purpose soy bean powder 30g, glucose 2g, sodium nitrate 2g, adding distil water is to 1000mL.
(3) take out cultured thalline zymotic fluid in (2),, get the radioresistant cocci wet thallus in the centrifugal 10min of 8000rpm, it is paved into the thickness of 1cm equably,, handled 50 seconds under the condition of electric-field intensity 9500v/m at frequency 980MHZ, make clasmatosis, treated radioresistant cocci is placed material disc, put into and carry out cold air drying in the cold wind drier, chilling temperature is controlled at 35 ℃, wind speed 3 meter per seconds, relative humidity 30% is dried to water content below 6%, obtains the radioresistant cocci through fragmentation.
2, compatibility combination
Will be through the radioresistant cocci of fragmentation and folic acid and three kinds of materials of biotin in mass ratio ratio combination in 1: 50: 200, and above-mentioned compound mixed, a kind of feed addictive that improves fertility of sow obtained.
3, effect
Control group adopts basal diet, and test group is added the feed addictive that 0.1% (adding the 0.1kg additive in every 100kg basal diet) pressed the raising fertility of sow of present embodiment method production in basal diet.Experimental period, begin to begin to the gestation of following one-period from nonpregnant sows, totally 60 of test sows, and every group each 30, kind is that big * is long, the parity unanimity.Effect sees Table 9.
Table 9: feed addictive of the present invention is to the influence of fertility of sow
| Produce the young number of living | Weight of weaning litter (Kg) | The rate % that oestruses in back 7 days weans | |
| Control group | 10.57±1.55A | 66.64±9.75A | 86.67 |
| Test group | 11.77±1.25B | 78.13±8.30B | 93.33 |
Annotate: annotate different capitalizations with delegation's acceptance of the bid and represent difference extremely significantly (P＜0.01).
Claims (5)
1. feed addictive that improves fertility of sow, it is characterized in that described feed addictive by radioresistant cocci (Deinococcus radiodurans) after break process, with quality for through the folic acid of 1～250 times of the radioresistant cocci of break process or composite the making of mixture of biotin or folic acid and biotin;
Described radioresistant cocci break process method is as follows:
(1) with the solid medium of radioresistant cocci bacterial classification inoculation to suitable radioresistant cocci, cultivate 32～36h for 30～32 ℃, picking list colony inoculation is to the fluid nutrient medium that is fit to radioresistant cocci, and 30～32 ℃ are cultured to exponential phase;
(2) step (1) is obtained liquid seeds by volume 5.1%～6.0% inoculum concentration be seeded to the fermentation medium that is fit to radioresistant cocci, cultivate 45～48h under ℃ condition of pH6.0～8.0,30～32;
(3) get the zymotic fluid that step (2) obtains, centrifugal, the radioresistant cocci wet thallus is tiled into 0.1cm～1.0cm thickness, under frequency 600～980MHz, electric-field intensity 8000～9500v/m condition, handle 30～50s, cold air drying obtains the radioresistant cocci through break process to water content below 6% under 5～35 ℃, wind speed 2～3m/s, relative humidity 10～30% conditions.
2. feed addictive as claimed in claim 1 is characterized in that described feed addictive is made up of the radioresistant cocci and the folic acid through break process of mass ratio 1: 1～5.
3. feed addictive as claimed in claim 1 is characterized in that described feed addictive is made up of the radioresistant cocci and the biotin through break process of mass ratio 1: 10～30.
4. feed addictive as claimed in claim 1 is characterized in that described feed addictive is made up of the radioresistant cocci through break process, folic acid and the biotin of mass ratio 1: 30～50: 100～200.
5. as the described feed addictive of one of claim 1～4, it is characterized in that described feed addictive addition in feed is 0.1～0.5%.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1445362A (en) * | 2003-04-09 | 2003-10-01 | 浙江大学 | Method for increasing yield of superoxide dismutase of radioresistant coccus by radiation induction |
| CN1563407A (en) * | 2004-03-16 | 2005-01-12 | 浙江大学 | Preparation technique for producing natural carotenoid by using radioresistant coccus |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1445362A (en) * | 2003-04-09 | 2003-10-01 | 浙江大学 | Method for increasing yield of superoxide dismutase of radioresistant coccus by radiation induction |
| CN1563407A (en) * | 2004-03-16 | 2005-01-12 | 浙江大学 | Preparation technique for producing natural carotenoid by using radioresistant coccus |
Non-Patent Citations (3)
| Title |
|---|
| 吴媛媛.耐辐射球菌中两个抗氧化蛋白功能及类胡萝卜素制备方法研究.《中国优秀博士学位论文》.2007,100-110. * |
| 田允波，等.母猪繁殖中的几种重要的维生素.《云南畜牧兽医》.2002,(第4期),17-20. |
| 田允波等.母猪繁殖中的几种重要的维生素.《云南畜牧兽医》.2002,(第4期),17-20. * |
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