CN101313676A - Biology coating agent, preparation and application in control of flue-cured tobacco axillary bud thereof - Google Patents
Biology coating agent, preparation and application in control of flue-cured tobacco axillary bud thereof Download PDFInfo
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- CN101313676A CN101313676A CNA2007100930284A CN200710093028A CN101313676A CN 101313676 A CN101313676 A CN 101313676A CN A2007100930284 A CNA2007100930284 A CN A2007100930284A CN 200710093028 A CN200710093028 A CN 200710093028A CN 101313676 A CN101313676 A CN 101313676A
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- coating agent
- biological coating
- bud
- flue
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Abstract
The invention provides a biological coating agent containing active composition beta-phenylethyl alcohol. The biological coating agent is made through the following steps that: plant strains with strong biological activity and high yield are selected from plants through collection, separation and purifying cultivation; wheat sprout-inhibiting quick detection is carried out and then high-yield strains are screened out through repeated comparison; and the biological coating agent is made through cultivation, solvent extraction, concentration and drying. When the biological coating agent is used in the control of flue-cured tobacco axillary bud, the available rate can reach to 92 to 98 percent, and the entire manufacturing process is safe and nontoxic; moreover, the biological coating agent does not contain toxic compositions, thereby causing no environmental pollution and no residual toxicity along with low production cost and low price; in addition, the biological coating agent can also be used in flower and vegetable preserving, crop growing regulation and control so as to improve quality and increase output, and so on.
Description
Technical field
The invention belongs to technical field of bioengineering, specifically, relate to and utilize the microbial fermentation principle, the technology that from secondary metabolite, filters out active component and be applied.
Background technology
Flue-cured tobacco is one of economic crops of more than 100 national extensively plantation in the world, and binding in the field cultivation management pruned, and operation is extremely tired requires great effort.For improving yield of tobacco and alleviating fatigue, since the 1950's, many countries are the axillary bud growth of applied chemistry medicament inhibition successively all.China is since the eighties, and from external introduction Flumetralin (maleic Min), Maleichydrazide (maleic hydrazide, bud enemy), Pendimethalin (it is logical to remove bud), Butradlin tests such as (ending the bud element) are used.The bud inhibition effect of these medicaments is each has something to recommend him, but all is the organic synthesis preparation, and residual toxicity is arranged, and contaminated environment is influential to Flue-cured tobacco Quality, and cost is also higher, and interior absorption MH veriety is forbidden at present.In recent years; use all drawbacks of bringing for reducing or eliminating the chemical pesticide large-scale popularization; the cry that the people of various countries require to control public hazards protection environment grows to even greater heights; for this reason, international " environment and development conference " required at the beginning of 21 century, and world's biopesticide application percentage will be brought up to and account for 60% of overall agricultural chemicals; world average level is at present less than 10%; China only is 2%, and therefore, actively grinding creation thing preparation has been the task of top priority.
Summary of the invention
The problem to be solved in the present invention provides a kind of safer, has no side effect, and is free from environmental pollution, no residual hazard misgivings, and the biological coating agent of efficient and cheap the invention provides the preparation method and the application in the control of flue-cured tobacco axillalry bud of this biological coating agent simultaneously.
Biological coating agent provided by the invention, its active component are bata-phenethyl alcohol, and molecular weight is 122, and molecular formula is C
6H
5CH
2-CH
2OH, structural formula is
The preparation method of above-mentioned biological coating agent, be made up of the following step:
(1), from plant, gather the disease sample of Botrytis Cinerea Persr fungi CGMCCNO NO0357-1, select fresh, the typical sample of symptom carries out surface sterilization, wash with aqua sterilisa, carrying out monospore with separate tissue or the direct spore method of shaking again separates, purifying is cultivated, then according to indoor bacterial strain screening and technological process of production method, select biologically active strong, solid fermentation produce rate is 25%～30%, wheat presses down bud fast detecting test extract and is diluted to 1500 times of still effective bacterial strains, mutation breeding again, relatively filter out superior strain repeatedly, number in regular turn, the bacterial classification access is boiled to half-mature sterilization barley corn, put into-40 ℃～-60 ℃ refrigerators and preserve standby;
(2), every liter of medium uses peeling potato 200 gram poach to filter, and adds glucose 10～15 grams, potassium dihydrogen phosphate 2.5～3 grams, animal protein peptone 1 gram, Cobastab
15 milligrams, citric acid 0.1 gram shakes up, and the room temperature jolting was cultivated 8～10 days, and ethyl acetate extraction extracting 2 times concentrates, and anhydrous sodium sulfate dehydration gets slightly takes out product bata-phenethyl alcohol, p-hydroxyphenylethanol etc., puts into-80 ℃ of low temperature refrigerators and preserves standby;
(3), products therefrom is adopted inhibition wheat bud scale growth method, carry out indoor quick bio and measure screening, with extract in advance to be measured to take out slightly that product dilute be 100 times, 200 times ... each 5 milliliters of 1200 times of series concentration move into and are placed with in the beaker of the wheat seed of handling, and are advisable with submergence seed just, the contrast clear water, each concentration repeats 3 times, puts in 26 ℃ of incubators and cultivates 7 days, filters out active strong, the strain culturing gained crude product bata-phenethyl alcohol that productive rate is high, and provide living test to test;
(4), screen active higher component, use column chromatography and TLC method purifying again, HPLC analyzes, and uses IRV, UV, MS, resolves in conjunction with the HNMR instrument, determines molecular weight, molecular formula and the structural formula of active component bata-phenethyl alcohol through give birth to surveying.
The axillalry bud that the biological coating agent is used for the flue-cured tobacco cultivation process is controlled, and adopts to drip to be coated with or to drench the mode of dripping and uses, and has reached the overgrowing of control axillalry bud, has received good bud inhibition effect.This coating agent also can be used for binding of other plant prunes, and also can be used for flowers, fresh-keeping of vegetables, and the regulation and control crop growth improves quality, improves output etc.Owing to biological coating agent of the present invention be from plant, gather, separate, cultivate, extraction, filter, dry getting, whole process safety non-toxic, do not contain poisonous composition, thereby free from environmental pollution, no residual hazard, production cost is low, and low price, cost per mu only need 8～9 yuan, can promote Flue-cured tobacco Quality again, control bud effect on average can reach 92%～98%.
Embodiment
Biological coating agent provided by the invention, its active component are bata-phenethyl alcohol, and molecular weight is 122, and molecular formula is C
6H
5CH
2-CH
2OH, structural formula is
When preparing above-mentioned biological coating agent, undertaken by following processing step:
(1), from plant, gather the disease sample of Botrytis Cinerea Persr fungi and (delivered China Committee for Culture Collection of Microorganisms common micro-organisms center in 1998, deposit number CGMCCNO NO 0357-1), select fresh, the typical sample of symptom carries out surface sterilization, wash with aqua sterilisa, carrying out monospore with separate tissue or the direct spore method of shaking again separates, purifying is cultivated, then according to indoor bacterial strain screening and technological process of production method, select biologically active strong, solid fermentation produce rate is 25%～30%, wheat presses down bud fast detecting test extract and is diluted to 1500 times of still effective bacterial strains, mutation breeding again, relatively filter out superior strain repeatedly, number in regular turn, the bacterial classification access is boiled to half-mature sterilization barley corn, put into-40 ℃～-60 ℃ refrigerators and preserve standby;
(2), every liter of medium uses peeling potato 200 gram poach to filter, and adds glucose 10～15 grams, potassium dihydrogen phosphate 2.5～3 grams, animal protein peptone 1 gram, Cobastab
15 milligrams, citric acid 0.1 gram shakes up, and the room temperature jolting was cultivated 8～10 days, and ethyl acetate extraction extracting 2 times concentrates, and anhydrous sodium sulfate dehydration gets slightly takes out product bata-phenethyl alcohol, p-hydroxyphenylethanol etc., puts into-80 ℃ of low temperature refrigerators and preserves standby;
(3), products therefrom is adopted inhibition wheat bud scale growth method, carry out indoor quick bio and measure screening, with extract in advance to be measured to take out slightly that product dilute be 100 times, 200 times ... each 5 milliliters of 1200 times of series concentration, immigration is placed with in the beaker of the wheat seed of handling, be advisable with submergence seed just, the contrast clear water, each concentration repeats 3 times, put in 26 ℃ of incubators and cultivated 7 days, filter out active strong, the strain culturing gained crude product active matter that productive rate is high, for example, we are from lettuce, green onion, strawberry, separation screening to 7 superior strain in the gray mold of plants such as acid Chinese quince is according to the preceding method separation and Culture, extraction, concentrate, can obtain semifinished product provides living test to test;
(4), screen active higher component through giving birth to survey, use column chromatography and TLC method purifying again, HPLC analyzes, and uses IRV (infrared), UV (ultraviolet), MS (matter is general), resolve in conjunction with the HNMR instrument, determine molecular weight, molecular formula and the structural formula of active component bata-phenethyl alcohol.
Bata-phenethyl alcohol is always thought food additives preferably, and is of many uses, all is to use as natural perfume material all the time.The present invention finds that by experimental study its controlling plant germination has good effect as the flue-cured tobacco axillalry bud, on average controls the bud rate and reaches 95%, and the chemical type that surpasses present import use presses down the bud agent.
Common both at home and abroad at present employing organic chemistry synthesizes or produces the production bata-phenethyl alcohol with microbial fermentation, general saccharomyces cerevisiae or the Kluyveromyces sp of adopting when microbial fermentation is produced bata-phenethyl alcohol, phenyl alanine, fluorophenylalanine are produced benzyl carbinol through the fermentation conversion, raw materials used phenyl alanine, fluorophenylalanine cost an arm and a leg, the production cost height, unsuitable large-scale industrial production; Employing tobacco waste (offal, offal) is also arranged, suitably add medium, through the microbial fermentation degraded and change into benzyl carbinol, but microorganism used therefor still is a budding fungus with the lignin in the tobacco, pectin, polyphenol compound, and productive rate is not high enough.The present invention utilized microbial fermentation to produce bata-phenethyl alcohol, and used grape spore fungi Botrytis cinerea Persr was preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center in 1998, and deposit number is CGMCCNO NO 0357-1.
The bata-phenethyl alcohol that adopts microorganism (Botrytis Cinerea Persr) fermentation to prepare is used for the control research of flue-cured tobacco axillalry bud, still follow the way of indoor and outdoor combination, the at first some strains of potted plant flue-cured tobacco seedling in the greenhouse, bata-phenethyl alcohol is mixed with 0.5%, 1%, 2% then ... 8% series concentration, with the dispenser bottle spray of 1%～2% concentration preparation or pouring are dripped on the seedling respectively, it is withered promptly to can be observed the seedling lobus cardiacus after 24 hours, and Da Ye preserves from, 3 batches of repeated tests, unanimity proves that growth has the function of tagging strongly to said preparation to the flue-cured tobacco young shoot as a result.
Though the flue-cured tobacco seedling test confirms that bata-phenethyl alcohol is food additives; but control flue-cured tobacco axillary bud growth also there is good function; be mixed with the missible oil (bata-phenethyl alcohol 10～25% that contains effective composition 10～25% so can further process; solvent 10～25%; emulsifier 8～10%; exhibition powder 5%); for the regular field trial of flue-cured tobacco field period; concrete grammar is to grow at the land for growing field crops flue-cured tobacco to bind when pruning; select growth neat; the height basically identical; test in the essentially identical plot of fertilizing management; draw guard row all around; arrange sub-district district's group at random; every sub-district area 30～50 strain cigarettes; the sign of listing respectively; it is (logical as removing bud to adopt the domestic chemical type of generally applying to press down the bud agent; the bud spirit of going out; maleic Min; bud is clean; end bud element etc.) make standard control; biological coating agent and chemistry are pressed down the bud agent with the field alternately; repeat four times; by using book separately water is used; 40 days at interval; organize relevant its effect of testing crew joint investigation; 5 samplings at random; calculate control bud percentage respectively, pluck the bright bud of bud position in case of necessity and weigh.Through for many years, the multiple spot field trial shows, the effect of new bio coating agent control flue-cured tobacco axillalry bud reaches 92%～98%, surpasses chemical type and presses down the bud agent.In addition, safe without toxic side effect, environmentally friendly, no residual hazard misgivings can promote the raising of yield of flue-cured tobacco quality again, market prospects are good.
Claims (3)
1, a kind of biological coating agent, the active component that it is characterized in that it is a bata-phenethyl alcohol, and molecular weight is 122, and molecular formula is C
6H
5CH
2-CH
2OH, structural formula is
2, the preparation method of the described biological coating agent of a kind of claim 1 is characterized in that comprising the following steps:
(1), from plant, gather the disease sample of Botrytis Cinerea Persr fungi CGMCCNO NO0357-1, select fresh, the typical sample of symptom carries out surface sterilization, wash with aqua sterilisa, carrying out monospore with separate tissue or the direct spore method of shaking again separates, purifying is cultivated, then according to indoor bacterial strain screening and technological process of production method, select biologically active strong, solid fermentation produce rate is 25%～30%, wheat presses down bud fast detecting test extract and is diluted to 1500 times of still effective bacterial strains, mutation breeding again, relatively filter out superior strain repeatedly, number in regular turn, the bacterial classification access is boiled to half-mature sterilization barley corn, put into-40 ℃～-60 ℃ refrigerators and preserve standby;
(2), medium uses peeling potato 200 gram poach to filter for every liter, add glucose 10～15 grams, potassium dihydrogen phosphate 2.5～3 grams, animal protein peptone 1 gram, 5 milligrams of vitamin B1s, citric acid 0.1 gram shakes up, and the room temperature jolting was cultivated 8～10 days, ethyl acetate extraction extracting 2 times, concentrate, anhydrous sodium sulfate dehydration gets slightly takes out product bata-phenethyl alcohol, p-hydroxyphenylethanol etc., puts into-80 ℃ of low temperature refrigerators and preserves standby;
(3), products therefrom is adopted inhibition wheat bud scale growth method, carry out indoor quick bio and measure screening, with extract in advance to be measured to take out slightly that product dilute be 100 times, 200 times ... each 5 milliliters of 1200 times of series concentration move into and are placed with in the beaker of the wheat seed of handling, and are advisable with submergence seed just, the contrast clear water, each concentration repeats 3 times, puts in 26 ℃ of incubators and cultivates 7 days, filters out active strong, the strain culturing gained crude product bata-phenethyl alcohol that productive rate is high, and provide living test to test;
(4), screen active higher component, use column chromatography and TLC method purifying again, HPLC analyzes, and uses IRV, UV, MS, resolves in conjunction with the HNMR instrument, determines molecular weight, molecular formula and the structural formula of active component bata-phenethyl alcohol through give birth to surveying.
3, the application of the described biological coating agent of claim 1 in flue-cured tobacco axillalry bud control adopted to drip to be coated with or to drench the mode of dripping, and is used for controlling the growth of axillalry bud.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100930284A CN101313676B (en) | 2007-11-23 | 2007-11-23 | Biology coating agent, preparation and application in control of flue-cured tobacco axillary bud thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100930284A CN101313676B (en) | 2007-11-23 | 2007-11-23 | Biology coating agent, preparation and application in control of flue-cured tobacco axillary bud thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101313676A true CN101313676A (en) | 2008-12-03 |
| CN101313676B CN101313676B (en) | 2011-10-12 |
Family
ID=40104933
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100930284A Expired - Fee Related CN101313676B (en) | 2007-11-23 | 2007-11-23 | Biology coating agent, preparation and application in control of flue-cured tobacco axillary bud thereof |
Country Status (1)
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|---|---|
| CN (1) | CN101313676B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103783087A (en) * | 2014-02-21 | 2014-05-14 | 重庆迎龙化工厂 | Preparation method for novel suckercide |
| CN113558050A (en) * | 2021-07-26 | 2021-10-29 | 周口师范学院 | Application of phenethyl alcohol in promoting plant growth, inducing plant disease resistance, inhibiting plant pathogenic bacteria growth and preventing and controlling plant diseases |
-
2007
- 2007-11-23 CN CN2007100930284A patent/CN101313676B/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103783087A (en) * | 2014-02-21 | 2014-05-14 | 重庆迎龙化工厂 | Preparation method for novel suckercide |
| CN103783087B (en) * | 2014-02-21 | 2017-01-25 | 重庆迎龙化工厂 | Preparation method for suckercide |
| CN113558050A (en) * | 2021-07-26 | 2021-10-29 | 周口师范学院 | Application of phenethyl alcohol in promoting plant growth, inducing plant disease resistance, inhibiting plant pathogenic bacteria growth and preventing and controlling plant diseases |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101313676B (en) | 2011-10-12 |
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Owner name: CHONGQING YINGLONG CHEMICALS CO., LTD. Free format text: FORMER OWNER: LIU LINGHUI Effective date: 20120518 |
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Effective date of registration: 20120518 Address after: Nanping Nan'an District of Chongqing City Road 400060 No. 7 Patentee after: Chongqing welcome Dragon Chemical Co., Ltd. Address before: Nanping Nan'an District of Chongqing City Road 400060 No. 7 building two floor B Patentee before: Liu Linghui |
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Granted publication date: 20111012 Termination date: 20191123 |