CN101310020A - Methods for genetic control of insect infestations in plantsand compositions thereof - Google Patents

Abstract

The present invention relates to methods for controlling pest infestation using double stranded RNA molecules. The invention provides methods for producing transgenic cells expressing the double stranded RNA molecules, as well as compositions and commodity products containing or treated with such molecules.

Description

Be used for method and composition at plant Genetic Control insect infestations

The sequence table of submitting on CD is attached to herein by reference

A CD (copy 1) with and xcopy (copy 2) on submitted sequence table to, each file is all set up on September 15th, 2006, all contains a 669kb file of called after " MNDE002.APP.TXT. ".Be attached to herein by reference in this content that contains on CD.

Right of priority

The application requires the U.S. Provisional Application series No.60/718 of submission on September 16th, 2005, and 034 right of priority, this application are quoted by integral body and be attached to herein.

Background of invention

1. invention field

Generally speaking, the present invention relates to the Genetic Control of insect invasion and attack.More specifically, prevent after the present invention the present invention relates to be used for transcribing or suppress the target encoding sequence in the expression of insect cell so that the recombinant DNA technology of insect protective effect to be provided.

2. the description of association area

The environment of human survival has been full of the insect invasion and attack.Insect comprises: insect, spider, Crustacean, fungi, bacterium, virus, nematode, flatworm, roundworm, pinworm, hookworm, tapeworm, taper worm, schistosomicide, horse botfly, flea, flat lice, mite, lice etc., they are ubiquitous in the human environment, have used the invasion and attack of these insects of multiple means attempt control.The composition that is used to control micro pest (for example bacterium, fungi and virus) invasion and attack has been provided as antibiotic compound, antiviral composition and antifungal composition form.(for example be used for control than major pest, nematode, flatworm, roundworm, pinworm, heartworm, tapeworm, taper worm, schistosomicide etc.) composition of invasion and attack typically exists as the form of chemical composition, they can be applied to the material surface that the insect invasion and attack appear in known meeting, are perhaps absorbed by affected animal with forms such as pill, powder, tablet, ointment or capsules.The present invention relates to provides: than composition known in the art, be used for improving one's methods of Pest Control invasion and attack.

Commercial agricultural farm crop are the target of attack of insect normally.In the past in the many decades, be used for the more efficiently method and composition of controlling plant insect infestations, had some substantial progress about exploitation.Chemical insecticide is very effective for eradicating the insect invasion and attack.But, use chemical insecticide that multiple shortcoming is arranged.Chemical insecticide is nonselective.People's intended application chemical insecticide is controlled deleterious invertebrate pests concerning multiple kinds of crops and other plant.But in default of selectivity, chemical insecticide also can apply its effect for non-target animals, and, answer exhausted for some time at chemical insecticide, can make the field barren usually.Chemical insecticide continues to be present in the environment, and is very slow in usually metabolism, if fully not by metabolic words.They can accumulate in food chain, particularly in the food chain of high zoophagous animal.The accumulation of these chemical insecticides causes the development of these reagent resistances and the high-end species of ladder that cause evolving, and described sterilant is had an effect as mutagenic compound and/or carcinogen, causes irreversible harmful genetic modification usually.Therefore people need be used for controlling or eradicate the insect infestations on plant or the plant strongly to the method for environment friendliness, promptly, optionally, environment inert, non-lasting reservation and biodegradable, and can be the method that is used for the pest resistance management system well.

The composition that comprises bacillus thuringiensis (B.t.) can obtain by commercial sources, and it has used above 30 years as environmentally safe and acceptable insecticide.The effect of insect extremely of Bt bacterium is the proteinic result of the proprietary generation of these bacteriums, described protein can not continue to be present in the environment, and, the selectivity that has height for affected target species, only by absorbed its effect that applies by the target insect, they have demonstrated the innocuousness to environment and other non-target biology (comprising the mankind).Contain the transgenic plant that kill proteic one or more genes of insect B.t. of encoding and also can obtain in this area, their significant effective are in the invasion and attack of control harmful insect.The substantive result who use to express the recombinant plant of Bt insecticidal protein is, in the area of using this type of transgenic crop, is applied to environment and obviously reduces with the amount of the chemical insecticide of control farm crop field insect invasion and attack.The minimizing that chemical insecticide is used makes soil more clean, and makes from the soil flow also cleaning more of water in streams, rivers, pond and lake towards periphery.Except that these environmental benefits, the farm crop field of the anti-insect crop growth of transgenosis, the quantity of favourable insect has remarkable increase, and this is because the cause that the use of chemical insecticide reduces.

Reported the antisense method and composition in this area, their are believed that can pass through single stranded RNA molecule (in theory, can in vivo with height complementary sense strand RNA molecular hybridization) synthetic applies its effect.Antisense technology is difficult to use in a lot of systems, and this has three major causes.At first, the antisense sequences instability of expressing in the transformant.The second, the unstable of the antisense sequences of expressing in the transformant is thereupon to giving this sequence the host, cell type or the biosystem that are delivered to away from transgenic cell to cause difficulty.The 3rd, pass the difficulty that runs into along with sending of unstable and antisense sequences and also made difficulty for following attempt, described attempt is: provide in the reconstitution cell inside of this antisense sequences of coding and can effectively regulate the dosage that target has the sense nucleotide sequence expression level.

The technology that is used for regulatory gene expression level in cell, tissue or biology rarely has improvement, especially, lacks the technology that is used for postponing, suppressing or reduce by the use recombinant DNA technology development of specific gene expression.In addition, as the result of the Unpredictability of these means, be not used in eucaryon or prokaryotic organism, regulate the specific gene expression level, the obtainable means of commercial sources.

Showed the inhibition of in various pests, specific gene being carried out double chain RNA mediate in the past.A kind of fruit bat, promptly in the drosophila melanogaster (Drosophila melanogaster) to be used for genetically controlled means detection (Kennerdell and Carthew, 1998 of dsRNA mediation in addition; Kennerdell and Carthew, 2000).Kennerdell and Carthew (1998) have described a kind of method, are used to send pass relate to the dsRNA that produces transgenic insect (it expresses double stranded rna molecule) or the dsRNA injection of solution is expelled in the egg capsule in insect body or before fetal development.

Show before the researchist, feed to raise by the solution that will contain two strands or siRNA molecule and be dipped in this solution, and, can in nematode, obtain the gene inhibition of double chain RNA mediate by injection dsRNA molecule to nematode or with nematode.People such as Rajagopal (2002) have described the trial of following failure: raise the solution that contains the dsRNA that is specific to target gene by feeding, or by larva being dipped in this solution, the native gene that suppresses harmful insect prodenia litura (Spodoptera litura) larva, but, after with little applicator (micro applicator) dsRNA being expelled to the hemolymph of five-age larva, people such as Rajagopal have but successfully obtained inhibition.Recently, people (2006) such as Yadav has reported that the dsRNA of the host's preparation that produces can prevent the plant infection nematode in plant.Similarly, U.S. Patent Application Publication No.2003/0150017 has predictably described and has been used to use the preferred gene seat that send the dsRNA that is delivered to larva to suppress lepidopterous larvae bollworm (Helicoverpaarmigera), described send to pass by having absorbed be converted into the plant that produces dsRNA and realize.The CRW specificity dsRNA that provides in the food of corn rootworm (CRW) at crucial CRW gene has been provided WO 2005/110068.Provide dsRNA in external and plant in CRW food, the result is that the failure to thrive of CRW larva after taking in this food hinders or is killed, and some different genes have been proved this effect.

Therefore, need to identify effective nucleotide sequence, described sequence be used for by prevent, genetic expression that delay or alternate manner reduce specific coleopteran pest and regulatory gene is expressed improves one's methods, the purpose of this method is the Pest Control invasion and attack or induces new phenotypic character.

Summary of the invention

On the one hand, the invention provides the method that suppresses expression of target gene in the coleopteran pest.In certain embodiments, this method comprises regulates or suppresses one or more target gene expression in the coleopteran pest, and described method can cause ingesting, grows, growth, breeding and/or infectivity stop, and finally causes insect death.Described method comprises: with the double-stranded RNA (dsRNA) of stabilization, (for example comprise its modified forms, siRNA (siRNA) sequence) (for example partly or entirely be incorporated into coleopteron cells in vivo or extracellular environment, midgut) in, wherein dsRNA enters into cell, and suppress at least a or multiple target gene expression, and wherein this is suppressed at and has applied deleterious effects on the coleopteran pest.Method of the present invention and compositions related can being used for provide described herein one or more to comprise the composition of dsRNA molecule by the food insect, restriction or eliminate the invasion and attack of coleopteran pest on environment inside that any insect host, insect symbiote or insect exist or surface.This method is useful especially for preventing the attack of insect plant.In one embodiment, insect to be defined as the pH of Digestive tract be about 4.5 to about 9.5, about 5 to about 9, about 6 to about 7 and about pH 7.0.

On the other hand, the invention provides the Exemplary core acid composition, at least a portion homology of one or more natural acid sequences in itself and the target insect.In certain embodiments, insect is selected from chrysomelid species (Diabrotica sp.), comprise western corn rootworm (WCR, Diabrotica virgifera or Diabrotica virgifera virgifera), south corn rootworm (SCR, Diabroticaundecimpunctata howardi), zea mexicana rootworm (MCR, Diabrotica virgiferazea), Brazil corn rootworm (BZR, Diabrotica balteata, Diabrotica viridula, Diabrotica speciosa), northern corn rootworm (NCR, Diabrotica barberi), Diabroticaundecimpunctata; And the Colorado colorado potato bug (CPB, Leptinotarsadecemlineata), red flour beetle (RFB, Tribolium castaneum) and Mexico's beans first (Epilachna varivestis).In other embodiments, insect is selected from lepidopterous insects, comprise European corn borer (ECB, Ostrinia nubilalis), black cutworm (BCW, Agrotis ipsilon), maize army worm (CEW, Helicoverpa zea), autumn noctuid (FAW, Spodopterafrugiperda), boll weevil (BWV, Anthonomus grandis), silkworm (Bombyxmori) and maduca sexta (Manduca sexta), and dipteral insect, comprise drosophila melanogaster (Drosophila melanogaster), malarial mosquito (Anopheles gambiae) and Aedes aegypti (Aedes aegypti).The specific examples of described nucleic acid provided by the invention is shown in the SEQ ID NO:1-SEQ ID NO:906 in the appended sequence table.

On the other hand, the invention provides the method for the genetic expression that is used to suppress coleopteran pest such as corn rootworm or relevant species, this method is included at least a dsRNA molecule of transcribing from above-described nucleotide sequence, its at least one fragment and the complementation of the intracellular mRNA sequence of insect that the gene inhibition amount is provided in the food of insect.This method may further include death, the inhibition of ingesting of observing insect, is obstructed or stops.The dsRNA molecule that insect is ingested comprises its modified forms, as the siRNA molecule, can have about 80 with the RNA molecule of transcribing from the nucleotide sequence that is selected from SEQ ID NO:1-SEQ IDNO:906,81,82,83,84,85,86,87,88 89,90,91,92,93,94,95,96,97,98,99 or about 100% identity.In specific embodiments, nucleotide sequence can be selected from SEQ ID NO:697, SEQ IDNOs:813-819, SEQ ID NO:841 and SEQ ID NO:874.

Therefore, in another aspect of this invention, provide a component from the nucleotide sequence of purifying, shown in the SEQ ID NO:1-SEQ ID NO:906.The invention provides the dsRNA molecule of stabilization or the expression of one or more miRNA, be used for suppressing coleopteran pest from these sequences and fragment expression target gene.The dsRNA of stabilization, comprise that miRNA or siRNA molecule can comprise at least two encoding sequences, they have justice and antisense orientation to arrange with respect at least one promotor, the nucleotide sequence that wherein comprises sense strand and antisense strand is connected by the intervening sequence at least about about 1000 Nucleotide of 5-or links to each other, wherein sense strand can be different length with antisense strand, and any one shown in each sequence in wherein said two encoding sequences and the SEQ ID NO:1-SEQID NO:906 or a plurality of nucleotide sequence have at least 80% sequence identity, at least 90%, at least 95%, at least 98%, or 100% sequence identity.

The fragment or the concatermer of the nucleotide sequence that is selected from SEQ ID NO:1-SEQ ID NO:906 have been the present invention further provides.In specific embodiments, nucleotide sequence can comprise the fragment or the concatermer of the sequence that is selected from SEQID NO:697, SEQ ID NOs:813-819, SEQ ID NO:841 and SEQ ID NO:874.

When being expressed as dsRNA and offering insect, this fragment can be defined as and cause insect death, the inhibition of ingesting, is obstructed or stops.This fragment can for example comprise among the SEQ ID NO:1-SEQID NO:906 any one or a plurality of sequence or its complementary sequence at least about 19,21,23,25,40,60,80,100,125 or more a plurality of continuous nucleotide.The length that is used for the present invention's a kind of useful dna fragmentation is about 23 Nucleotide of about 19-, or about 100 Nucleotide of about 23-, about 2000 Nucleotide or more of as many as.Useful especially will be to comprise about 300 the dsRNA sequences with insect target sequence homologous Nucleotide of about 23-.The present invention also provides the Yeast Nucleic Acid of expressing from any described sequence, comprises dsRNA.Can be from select to be used for the sequence of expressing gene inhibitor from the single sequence construct of one or more target insects, and be used for the individual gene of one or more target insects of expression inhibiting or the RNA of gene family, or this dna sequence dna can be configured to mosaic from multiple dna sequence dna.

On the other hand, the invention provides the recombinant DNA construction body, it comprises the nucleic acid molecule of the dsRNA molecule described herein of encoding.Can form dsRNA by from having a chain of transcribing the dsRNA molecule at least about the nucleotide sequence of about 100% identity of 80%-with the nucleotide sequence that is selected from SEQ ID NO:1-SEQ IDNO:906.Described recombinant DNA construction body can be orientated as and can suppress the dsRNA molecule that endogenous target gene is expressed after the absorption in insect.This construct can comprise the optional nucleotide sequence of the present invention that is connected with the promoter sequence that works in host cell.Described promotor can be tissue-specific, and can for example be specific to the types of organization that attacked by insect.For example, under the situation of rootworm, may need to use provides preferred root expression promoter.

Nucleic acid construct of the present invention can comprise the nucleotide sequence that at least one non-natural exists, and it can be transcribed into the single stranded RNA that can form the dsRNA molecule in vivo by hybridization.Described dsRNA sequence self assembling, and can be provided in the food of coleopteran pest, to reach the inhibition that needs.

The recombinant DNA construction body can comprise two kinds of different non-natural sequences, when described sequence is expressed as the dsRNA sequence in vivo and is provided in the food of coleopteran pest, suppresses at least two kinds of different target genes expression in the coleopteran pest cell.In certain embodiments, in comprising cell with the inhibiting cell of insect or plant, produce at least 3,4,5,6,8 or 10 or more kinds of different dsRNAs.A plurality of constructs that described dsRNA can introduce from different transformation events are expressed, and maybe can introduce on the single nucleic acid molecule.Can be with single promotor or a plurality of promoter expression dsRNA.In one embodiment of the present invention, produce single dsRNA, its comprise with insect in a plurality of locus homologous nucleic acid.

On the other hand, the invention provides recombinant host cell, have at least one recombinant DNA sequence in its genome, this sequence is transcribed and is produced at least one dsRNA molecule, and this molecule works when coleopteran pest is taken in, and suppresses target gene expression in the insect.The dsRNA molecule can be by any nucleic acid encoding shown in this paper description and the sequence table.The present invention also provides the transformed plant cells that has at least one recombinant DNA sequence described herein in the genome.The transgenic plant that comprise described transformed plant cells also are provided, have comprised progeny plants, seed and the plant prod in any generation, they all comprise recombinant DNA.

Method and composition of the present invention can be applicable to any unifacial leaf and dicotyledons, and this depends on the coleopteran pest control of wanting.Especially, plant includes but not limited to: clover, dill (aneth), Apple, apricot, choke, rocket salad, asparagus, avocado, banana, barley, Kidney bean, beet, blackberry, blueberry, blueberry, cabbage, brussels sprouts, Caulis et Folium Brassicae capitatae, Corolla (canola), muskmelon, Radix Dauci Sativae, cassava, Cauliflower, celery, cherry, coriander, oranges and tangerines, the little oranges and tangerines of Ke Laimenshi, coffee, corn, cotton, cucumber, Pseudotsuga menziesii (Mirbel) Franco, eggplant, hare's-lettuce, wide leaf lettuce, eucalyptus, fennel, Fructus Fici, cucurbit, grape, shaddock, hami melon, yam bean, kiwi fruit, lettuce, leek, lemon, bitter orange, torch pine, mango, melon, mushroom, nut, oat, okra, onion, tangerine, decorate and use plant, pawpaw, parsley, pea, peach, peanut, pears, pepper, persimmon, pine tree, pineapple, plantain, Lee, pomegranate, white poplar, potato, pumpkin, Wen Bai, pine, witloof, radish, raspberry, paddy rice, naked barley, Chinese sorghum, the south pine, soybean, spinach, pumpkin, strawberry, sugar beet, sugarcane, Sunflower Receptacle, Ipomoea batatas, Styrax, mandarin orange, tea, tobacco, tomato, turf, trailing plant, watermelon, wheat, Chinese yam and Xiao Hu's melon plant.Therefore, the present invention also provides recombinant DNA sequence or its concatermer, fragment or the complementary sequence plant transformed shown in the SEQ ID NO:1-SEQ ID NO:906, and described sequence is transcribed and produced at least a dsRNA molecule that suppresses expression of target gene in the insect when coleopteran pest absorbs.In specific embodiments, recombinant DNA sequence can be selected from SEQ ID NO:697, SEQ ID NOs:813-819, SEQ IDNO:841 and SEQ ID NO:874 or its fragment, complementary sequence or concatermer.

The present invention also provides the combination of the method and composition that is used to control the coleopteran pest invasion and attack.A kind of means provide and have been used for protective plant and prevent dsRNA method insect infestations, as herein described, and one or more insecticides, and described insecticide shows the different feature of feature that is shown with the dsRNA method and composition.For example, can provide one or more Bt albumen in the food of insect pest, itself and this paper describe one or more dsRNA combinations.Be formulated as the composition of topical application, or the composition that dsRNA method and composition and Bt method and composition are combined that uses transgenic method to obtain, can produce synergistic effect, this be in the field of control insect infestations before the unknown.A kind of synergistic effect is that the required expression level of dsRNA or Bt albumen reduces.When being grouped together, every kind of agent for controlling noxious insect pests all only needs lower effective dose.Thinking that the Bt insecticidal protein has been set up enters the hole, the dsRNA molecule can more effectively penetrate into space away from insect gut by the described hole that enters, perhaps more effectively enter near the cell of damage that Bt albumen is set up, only need the Bt of less amount or the insect result extremely that dsRNA just can obtain to want thus, inhibition or the containment perhaps wanted to target insect target biological function.

Therefore, the invention provides a kind of composition, wherein contain two or more different sterilants, wherein every kind all poisonous to same insect or insect species, at least a this paper of comprising wherein describes dsRNA.In certain embodiments, second kind of sterilant can be selected from down group: potato tuber storage protein (patatin), bacillus thuringiensis insecticidal albumen, Xenorhabdus insecticidal protein, polished rod bacterium insecticidal protein, bacillus laterosporus insecticidal protein, Bacillus sphaericus insecticidal protein and xylogen.Bacillus thuringiensis insecticidal albumen can be any kind of in a large amount of insecticidal proteins, described insecticidal protein includes but not limited to: Cry1, Cry3, TIC851, CryET70, Cry22, TIC901, TIC1201, TIC407, TIC417, binary insecticidal protein CryET33 and CryET34, binary insecticidal protein CryET80 and CryET76, binary insecticidal protein TIC100 and TIC101, binary insecticidal protein PS149B1, the VIP insecticidal protein, TIC900 or associated protein, or the combination of insecticidal protein ET29 or ET37 and insecticidal protein TIC810 or TIC812, and the insect mosaic extremely of any aforementioned insecticidal protein.

The Yeast Nucleic Acid that provides in food can be provided among the artificial food, and this food preparation is used to satisfy the special nutrition requirement that insect keeps described food.Can replenish the RNA of insect manipulated variable in this food, this RNA is purifying from the expression system that separates, and absorbs through the inhibition level of activity after the food that replenishes with RNA composition or the definite insect of determining the insect manipulated variable.This food also can be the reconstitution cell that transforms with dna sequence dna, and described dna sequence dna makes up and is used to express reagent, RNA or gene inhibition agent.After insect absorbs one or more described transformants, observe the phenotype result who needs, show that this reagent suppresses the expression of the intracellular target nucleotide sequences of insect.

By the necessary albumen of gene codified that target suppresses, described proteic expectation function is selected from: muscle forms, the formation of neotonin, the adjusting of neotonin, ion are regulated and transhipment, the synthetic and transhipment of albumen, digestive ferment is synthetic, cell membrane potential keeps, and amino acid bio is synthetic, amino acid degradation, sperm forms, and pheromone is synthetic, the pheromone sensing, feeler forms, and wing forms, and leg forms, grow and differentiation, avette one-tenth, the larva maturation, digestive ferment forms, hemolymph is synthetic, the maintenance of hemolymph, neurotransmission, cell fission, energy metabolism, breathe unknown function, and apoptosis.

Another aspect of the present invention also provides the method that is used to improve the crop yield that the farm crop that are subjected to insect pest invasion and attack produce, and said method comprising the steps of: a) will comprise the polynucleotide of the sequence that is selected from SEQID NO:1-SEQ ID NO:906 or its complementary sequence or concatermer or fragment and introduce described crop plants; And b) cultivates described crop plants so that described polynucleotide are expressed, the ingesting and because the production loss that the insect invasion and attack cause of the expression inhibiting insect pest of wherein said polynucleotide.

In certain embodiments, the expression of polynucleotide produces the RNA molecule, and at least the first target gene in the insect pest of a part of described crop plants has been absorbed in its inhibition, wherein this target gene is exercised at least a household function that is selected from down group: insect ingests, insect viability, insect apoptosis, the differentiation of insect or any insect cell and growth, the sexual propagation of insect, muscle forms, muscle twitch, Muscle contraction, the formation of neotonin and/or minimizing, the adjusting of neotonin, ion is regulated and transhipment, the maintenance of cell membrane potential, and amino acid bio is synthetic, amino acid degradation, sperm forms, and pheromone is synthetic, the pheromone sensing, feeler forms, and wing forms, leg forms, avette one-tenth, larva maturation, digestive ferment forms, hemolymph is synthetic, and hemolymph keeps, neurotransmission, larval stage changes, and pupates, broken pupa, cell fission, energy metabolism is breathed, synthetic and the maintenance of cytoskeletal structure, nucleotide metabolism, nitrogen metabolism, water conservancy is used, water keeps, and sensory perception.

In other embodiments, insect pest is the corn rootworm insect, be selected from: Diabroticaundecimpunctata howardi (southern corn rootworm (SCR)), Diabrotica virgiferavirgifera (western corn rootworm (WCR)), Diabrotica barberi (northern corn rootworm (NCR)), Diabrotica virgifera zea (zea mexicana rootworm (MCR)), Diabroticabalteata (Brazilian corn rootworm (BZR)), Diabrotica viridula (Brazilian corn rootworm (BZR)) and Diabrotica speciosa (Brazilian corn rootworm (BZR)).

The method of the arid tolerance that improves the farm crop that produced by the crop plants that is subjected to the insect pest invasion and attack also is provided, and said method comprising the steps of: polynucleotide sequence or its fragment that a) will be selected from SEQ ID NO:1-SEQ ID NO:906 are introduced described crop plants; And b) cultivate described crop plants so that described polynucleotide are expressed, the expression inhibiting insect pest of wherein said polynucleotide ingest and because the arid tolerance that the insect invasion and attack cause is impaired.

Another aspect of the present invention further provides agriculture and has gone up and commercially important product and/or composition, include but not limited to, animal-feed, agricultural-food and be intended to as the human food that consumes or be used for the human composition that consumes and the product and the byproduct of agricultural-food include but not limited to Semen Maydis powder, hominy grits, corn steep liquor, Semen Maydis oil, W-Gum, puffed rice, corn-dodger, cereal etc.But these compositions can contain the nucleotide sequence shown in this paper of detection limit, therefore also can diagnose any transgenic event that contains this type of nucleotide sequence.These products are useful, and at least because they may derive from the farm crop that breed under the condition of still less sterilant and organophosphate, this is because they have mixed Nucleotide of the present invention and have been used for controlling the result of coleopteran pest in the invasion and attack of plant.This agricultural products and agricultural-food goods are to produce from the seed that is produced by transgenic plant, wherein, described transgenic plant expressed rna, described RNA is from one or more continuous nucleotides of the present invention, or the Nucleotide of one or more coleopteran pests and complementary sequence thereof.This agricultural products and agricultural-food goods also can be used for controlling the coleopteran pest of this agricultural products and agricultural-food goods, for example, surface powder weevil, because have the RNA that can suppress the insect gene in agricultural-food or agricultural-food goods, described RNA is that gene order shown in the present is expressed.

The method of producing described agricultural-food goods also is provided, comprise obtaining with comprising the sequence that is selected from SEQ IDNO:1-SEQ ID NO:906 or the polynucleotide plant transformed of its concatermer or fragment or complementary sequence, and prepare the agricultural-food goods from described plant or its part.In addition, another aspect of the present invention is the method for producing food or feed, comprise the polynucleotide or its fragment or the complementary sequence plant transformed that obtain with being selected from SEQ ID NO:1-SEQ ID NO:906, and prepare food or feed from described plant or its part.

The present invention also provides computer-readable medium, record one or more or its complementary sequence in the nucleotide sequence shown in the SEQ ID NO:1-SEQ ID NO:906 thereon, being used for a large amount of computer baseds uses, include but not limited to, DNA identity and similarity retrieval, protein identity and similarity retrieval, transcribe that stave is levied, comparison and artificial hybridization analysis between the genome.

The accompanying drawing summary

Fig. 1: the biological assay of the F1 maize plant incident that transforms and attack with western corn rootworm (WCR) with pMON98503 (SEQ ID NO:820).

Fig. 2: the biological assay of the F1 maize plant incident that transforms and attack with western corn rootworm (WCR) with the pMON98504 that comprises concatermer C1 (SEQ ID NO:821).

The schematic design of segmental selection of Fig. 3: Dv49 and Dv248 and Dv49-Dv248 concatermer C38.

Fig. 4: based on concatermer C38 synthetic dsRNAs F1-F13.

Fig. 5: DV49-DV248 concatermer 38 dose responses (fragment F1-F6).

Fig. 6: DV49-DV248 concatermer 38 dose responses (fragment F7-F10).

Fig. 7: DV49-DV248 concatermer 38 dose responses (fragment F11-F13).

Detailed Description Of The Invention

Hereinafter be detailed description of the present invention, it is provided to assist those skilled in the art to put into practice the present invention. Those of ordinary skills can not leave and make improvement in the situation of aim of the present invention or scope and change in enforcement mode as herein described.

The invention provides the method and composition for hereditary Control pests invasion and attack. For example, the invention provides recombinant DNA technology, prevent or suppress the hit expression of coded sequence of insect cell after being used for transcribing, thereby provide the insect protective action, the method is by making insect ingest one or more all or part of two strands of transcribing or small interference ribonucleic acid (RNA) molecules from the target coded sequence, thus the control invasion and attack. Therefore, the present invention relates to double-stranded RNA (dsRNA), comprise that siRNA (siRNA) carries out sequence-specific to the expression of coded sequence and suppresses, to reach the insect control level of expection.

The nucleic acid molecules that separates with basic purifying is provided, include but not limited to nucleotide sequence and recombinant DNA construction body that non-natural exists, be used for transcribing the dsRNA molecule of thing invention, interior source coding sequence in the insect or the expression of target coded sequence are prevented or suppressed to described sequence and construct when introducing. Genetically modified plants also are provided, they (a) thus comprise separation and the nucleic acid molecules of basic purifying and the nucleotide sequence of the recombinant DNA construction body that non-natural exists that coding is used for transcribing the invasion and attack of dsRNA molecule control plant insect, and (b) show the resistance of insect infestations and/or the tolerance of enhancing. Also described the composition that contains dsRNA nucleotide sequence of the present invention, be used for surface applied to plant or animal is upper or be administered to the environment of animal, to realize elimination or the minimizing of insect invasion and attack.

The inventor finds, opposite with the instruction of prior art, coleoptera species (obtaining nucleotide sequence by described species) are ingested contain the composition of the double stranded rna molecule that the sequence that exists in the nucleotide sequence by one or more expression of coleoptera species forms, cause the inhibition of one or more biological functions in the coleoptera species. Especially, the inventor finds to make crop pests species such as the corn rootworm double stranded rna molecule described herein of ingesting, and causes taking in insect pest dead of these compositions or grows and differentiation suppresses.

The inventor has identified that nucleotide sequence described herein provides the protective effect of anti-coleopteran pest species. The amino acid sequence of the cDNA sequence of having derived coding, and compare with known amino acid sequence. Predict that many sequences in these sequences all encode and have the albumen of more relative annotation information. Be based on amino acid sequence that the translation by coded sequence described herein derives and homology or the similitude between the amino acid sequence known in the art in the public database with specific nucleotide sequence with by the relevant annotation information of its Protein sequence.

Select the cDNA sequence of the coding necessary albumen of survival or protein part, for the preparation of the double stranded rna molecule that provides in the food of coleopteran pest, described albumen is such as the amino acid that participates in multiple metabolism or catabolism biochemical route, cell division, breeding, energetic supersession, digestion, nervous system function etc. Description such as this paper, the target insect is taken in the composition that contains one or more dsRNA (its at least one fragment is corresponding at least one the essentially identical RNA fragment that produces in the target insect cell), causes death, hypoevolutism or other inhibition of target insect. These results show that by the nucleotide sequence that coleopteran pest obtains, namely DNA or RNA can be used for making up the plant cell that anti-insect attacks. For example, can transform the insect host, make it contain the nucleotide sequence that one or more derive from coleopteran pest. Be transformed into nucleotide sequence in insect host or the homobium and can be coded in one or more RNA that form dsRNA in the host of conversion or the cell in the homobium or the biological fluid, if thereby/when insect when transformed host or homobium are ingested, can there be dsRNA in the food of insect, cause the expression of one or more genes in the insect cell to be prevented, and finally cause dead, slow or other inhibition of growing of insect.

The present invention relates generally to the heredity control of coleopteran pest invasion and attack in the host living beings. More specifically, the present invention includes for agent for controlling noxious insect pests being sent the method for passing coleopteran pest. Described agent for controlling noxious insect pests directly or indirectly cause insect to keep self growth or the ability of target host or homobium of otherwise attacking impaired. The invention provides for the dsRNA molecule that adopts stabilisation at pest food as preventing the method for the means of target gene in the insect, thereby realized in host that the insect target is fixed or the homobium or about the required insect invasion and attack control of described host or homobium.

In the process that realizes aforementioned purpose, the invention provides the inhibition coleopteran pest, the method that comprises expression of target gene in corn rootworm for example or other coleopteron species causes the ingesting of insect, grows, the stopping of growth, breeding, appeal, and finally can cause insect dead. In one embodiment, the method comprises the alimentation composition that double-stranded RNA (dsRNA) nucleic acid molecule of stabilisation partially or completely is introduced as the pest food source, and makes insect can access described alimentation composition to be used for ingesting. The alimentation composition that absorption contains two strands or siRNA molecule causes insect cellular uptake molecule, causes the inhibition of at least a expression of target gene in the insect cell. The inhibition of target gene applies harmful effect to insect.

In certain embodiments, dsRNA molecule provided by the invention comprise with SEQ ID NO:1-SEQ ID NO:906 in the nucleotide sequence of sequence complementation shown in any one, in the insect biology among the SEQ ID NO:1-SEQ ID NO:906 inhibition of sequence shown in any one cause the insect growth and grow or minimizing or the removal of the necessary albumen of other biological function or nucleotide reagent. The nucleotide sequence of selecting can with one of nucleotide sequence shown in the SEQ ID NO:1-SEQ ID NO:906 in the sequence table, comprise that its complementary series has the sequence homogeneity of about 80%-about at least 100%. Described inhibition can be described as specificity, because the nucleotide sequence of the part of target gene is selected from the sequence that inhibition property dsRNA or siRNA transcribe. The method can at least a target gene of establishment expression, and can be used for suppressing a lot of dissimilar target gene of insect. In specific embodiments, nucleotide sequence can be selected from SEQ ID NO:697, SEQ ID NOs:813-819, SEQ ID NO:841 and SEQ ID NO:874.

Can be expressed as easily the dsRNA molecule by setting up suitable expression construct, be accredited as the sequence with insect protective action. For example, by following step, described sequence can be expressed as hair clip and stem and ring structure: take out corresponding to the sequence that is selected from SEQ ID NO:1-SEQ ID NO:906 or the first fragment of its fragment, this sequence is connected in not and the first fragment homology or complementary second spacer segment district, and it is connected with the 3rd fragment of transcribe rna, wherein at least a portion of the 3rd fragment and this complementation of first segment base. Described construct forms stem and ring structure by the hybridization of the first fragment and the 3rd fragment, forms the ring structure (WO94/01550, WO98/05770, US 2002/0048814A1, and US 2003/0018993A1) that comprises the second fragment.

A. nucleic acid compositions and construct

The invention provides the recombinant DNA construction body for the stable conversion that realizes specific host or homobium insect target. The host who transforms or homobium insect target can be expressed extremely preferred dsRNA or the siRNA molecule from described recombinant DNA construction body of the effective level of worm, and described molecule is provided in the food of insect. Can be provided as right separation and the nucleotide sequence of purifying from cDNA library and/or genomic library information. Nucleotide sequence is to deriving from any preferred coleopteran pest, and is right as the heat amplification, to produce the DNA template for the preparation of dsRNA of the present invention and siRNA molecule.

Term used herein " nucleic acid " refers to the deoxyribose nucleotides of from 5 ' to 3 ' terminal reading code or list or the double-stranded polymer of ribonucleotide base. Optional ground, " nucleic acid " also can contain exist or the reformed nucleotide base of non-natural, and the correct reading code that it allows by the polymerization enzyme can not reduce the expression of the polypeptide of this nucleic acid coding. Term " nucleotide sequence " or " nucleotide sequence " refer to exist or be present in as independent strand the sense and antisense chain of the nucleic acid in the double-stranded body. Term " ribonucleic acid " (RNA) comprises RNAi (inhibitory RNA), dsRNA (double-stranded RNA), siRNA (siRNA), mRNA (courier RNA), miRNA (little RNA), tRNA (transhipment RNA, quilt or do not added electric charge by corresponding acylated amino) and cRNA (complementary RNA), and term " DNA " (DNA) comprises cDNA and genomic DNA and DNA RNA hybrid. Vocabulary " nucleic acid fragment ", " nucleic acid sequence fragments " or more common " fragment " will be interpreted as by those skilled in the art: it comprises the nucleotide sequence of genome sequence, rRNA sequence, transhipment RNA sequence, courier RNA sequence, operon sequence and less through engineering approaches, and described sequence is expressed maybe can be suitable for marking protein, polypeptide or peptide.

According to the present invention, nucleotide sequence is provided, its expression obtain with insect in the RNA sequence of the remarkable homology of RNA molecule of target gene, described insect comprises by the nucleotide sequence coded RNA sequence in the insect genes group. Therefore, after taking in the RNA sequence of stabilisation, can obtain the downward modulation to the nucleotide sequence of target gene in the insect cell, cause the harmful effect to the keeping of insect, viability, propagation, breeding and invasion and attack.

Term used herein " significantly homology " or " significantly homology ", when mentioning nucleotide sequence, be included under the strict condition with sequence table in the coded sequence shown in any one or the nucleotide sequence of its complementary sequence hybridization among the SEQ ID NO:1-SEQ ID NO:906. The sequence of any sequence of SEQ ID NO:1-SEQ ID NO:906 or its complementary sequence hybridization is the sequence that allows to occur between two sequences antiparallel arrangement under strict condition and in the sequence table, then two sequences can under the strict condition with the phase anti-chain on corresponding base form hydrogen bond, to form double-stranded body molecule, it is sufficiently stable under strict condition, uses approach well known to detect. Preferably, significantly has the sequence homogeneity of about 70%-about 80% with reference to nucleotide sequence or its complementary series shown in any one among the sequence of homology and the SEQ ID NO:1-SEQ ID NO:906 in the sequence table, perhaps more preferably, the sequence homogeneity of about 80%-about 85%, perhaps most preferably, the sequence homogeneity of about 90%-about 95%, the sequence homogeneity to about 99%.

Term used herein " sequence homogeneity ", " sequence similarity " or " homology " are used to describe the relation between the two or more pieces nucleotide sequence. The percentage of " sequence homogeneity " is measured by following method between the two sequences: the sequences that compare two optimum comparisons in comparison window, wherein, than canonical sequence (do not comprise and add or disappearance), in the comparison window part of sequence can comprise add or disappearance (namely, the room), be used for optimum comparison two sequences. Calculate percentage by following method: measure the quantity that has the position of identical nucleic acid base or amino acid residue in the two sequences, to produce the quantity of matched position, divided by the total number of positions in the comparison window, the result be multiply by 100 to produce the percentages of sequence homogeneity with the quantity of matched position. The identical sequence in each position is considered to identical with control sequence during with the canonical sequence comparison, and vice versa. If article one nucleotide sequence shows the complete complementary with second or canonical sequence, when observing with 5 ' to 3 ' direction, article one nucleotide sequence be considered to the second observed with 3 ' to 5 ' direction or with reference to the complementary series of nucleotide sequence or with its complementation. Herein, with complementary with each nucleotides of other sequence of 3 ' to 5 ' direction reading code the time, we say that the nucleotide sequence molecular display goes out " complete complementary " with each nucleotides in one of sequence of 5 ' to 3 ' direction reading code. To show the sequence identical with the reverse complementary sequence of reference nucleotide sequence with the nucleotide sequence of reference nucleotide sequence complementation. These terms and description have clear and definite definition in the art, are that those of ordinary skills are understandable.

" comparison window " used herein refers to, notional fragment of at least 6 continuous positions, common about 50 to about 100, more commonly, about 100 to about 150 continuous positions, wherein, after two sequences is compared by optimum, a sequence is compared with the canonical sequence with same quantity continuous position. Than canonical sequence (do not comprise add or disappearance), comparison window can comprise about 20% or interpolation still less or disappearance (that is, the room), is used for the optimum comparison of two sequences. Those skilled in the art are with reference to Wisconsin Genetics Software Package Release 7.0, Genetics Computer Group, 575 Science Drive Madison, Wis., be used for the method detailed of sequence comparison or the detailed discussion of analyzing about sequence with reference to the people such as Ausubel (1998) in USA).

The present invention also provides dna sequence dna, it can be expressed as RNA in cell or microorganism, in order to suppress the expression of target gene in insect cell, tissue or the organ, this sequence comprises the dna molecular of one or more different IPs nucleotide sequences of encoding, wherein, in the different IPs nucleotide sequence each comprises all that the interval sequence connects sense nucleotide sequence and antisense base sequences, the described sequence dsRNA molecule of the present invention of encoding. The interval sequence has consisted of the part that sense nucleotide sequence or antisense base sequences are arranged, and is formed in the dsRNA molecule between the sense and antisense sequence. There are nucleotide sequence or derivatives thereof or its complementary series of sense nucleotide sequence and antisense base sequences and target gene basic identical. The dsDNA molecule is operationally placed under the control of promoter sequence, and described promoter sequence plays a role in host's cell, tissue or organ, expresses dsDNA, produces the dsRNA molecule. In one embodiment, dna sequence dna can derive from the nucleotide sequence shown in the SEQ ID NO:1-SEQ ID NO:906 in the sequence table.

The present invention also provides dna sequence dna, is used at the plant cells, and, along with DNA is expressed as RNA and is absorbed by the target insect, can obtain preventing the expression that is harmful to target gene in insect cell, tissue or the organ. DsRNA comprises one or more structural gene sequences at least, and wherein, every kind of structural gene sequence comprises all that the interval sequence connects sense nucleotide sequence and antisense base sequences, and described sequence forms in complementary and antisense sequences and encircles. There are nucleotide sequence or derivatives thereof or its complementary series of sense nucleotide sequence and antisense base sequences and target gene basic identical. One or more structural gene sequences are operationally placed under the control of one or more promoter sequences, and are wherein at least a biological at protokaryon or eucaryon, particularly can operate in the cell of plant, tissue or the organ.

Gene order or fragment for Control pests of the present invention can be cloned into two kinds of organizing specific promoters that can operate at transgenic plant cells, for example between two kinds of root-specific promoters, described gene order or its fragment can expressed herein, produce mRNA in transgenic plant cells, it forms the dsRNA molecule. The dsRNA molecule that contains in the plant tissue is absorbed by insect, thereby obtains the expection of expression of target gene is prevented.

Nucleotide sequence provided by the invention can comprise the inverted repeats of being separated by " interval sequence ". The interval sequence can be the zone that comprises following any nucleotide sequence, and if necessary, described nucleotide sequence can promote each to repeat secondary structure formation between the sequence. In one embodiment of the invention, the interval sequence is the part of the sense or antisense coded sequence of mRNA. Perhaps, the interval sequence can comprise can with the covalently bound nucleotides of nucleic acid molecules or any combination of its homology thing. The interval sequence can comprise length and be the nucleotide sequence of about at least 10-100 nucleotides, and perhaps length is about at least 100-200 nucleotides, and length is about at least 200-400 nucleotides, and perhaps length is about at least 400-500 nucleotides.

Nucleic acid molecules in the sequence table or the fragment of nucleic acid molecules or other nucleic acid molecules can be under certain conditions specifically with other making nucleic acid molecular hybridization. If two molecular energies form antiparallel double-stranded nucleic acid structure, just say mutually specific hybrid of two nucleic acid molecules herein. If two nucleic acid molecules show complete complementary, we just say that a nucleic acid molecules is the complementary series of another nucleic acid molecules. If under traditional at least " low stringency " condition, two molecular energies allow them to remain the state of mutual annealing with the mutually hybridization of enough stability, and we just say that these two molecules are " minimum complementary ". Similarly, if under traditional " high stringency " condition, two molecular energies allow them to remain the state of mutual annealing with the mutually hybridization of enough stability, and we just say that these two molecules are complementary. Traditional strict condition is described in the people's such as the people such as Sambrook and Haymes document to some extent.

Therefore, have to deviate from complete complementation to allow, as long as thisly deviate from the ability completely dissolve that can not make molecule form duplex structure.Therefore,, only need sequence enough complementary, can under used specific solvent and salt concn, form stable duplex structure and get final product for the fragment that makes nucleic acid molecule or nucleic acid molecule is used as primer or probe.

Can promote the suitable stringent condition of DNA hybridization to be, for example, under about 45 ℃, 6.0x sodium chloride/sodium citrate (SSC), then wash with 2.0xSSC at 50 ℃, this is well known by persons skilled in the art, perhaps can find in Current Protocols in Molecular Biology (1989).For example, the salt concn in the washing step can be selected from: the low severity of 50 ℃ of following about 2.0xSSC, and to the high severity of 50 ℃ of following about 0.2xSSc.In addition, the temperature of washing step can be from room temperature, the high stringency of the low stringency under promptly about 22 ℃ under about 65 ℃.Temperature and salt can all change, and perhaps, it is constant that temperature or salt concn can keep, and other variable changes.Be used for nucleic acid of the present invention can be under the described conditions can with one or more nucleic acid molecule or its complementary sequence specific hybrid from WCR.Preferably, be used for nucleic acid of the present invention and will have about at least 80% with one or more nucleic acid molecule shown in the sequence table SEQ ID NO:1-SEQ ID NO:906, perhaps about at least 90%, perhaps about at least 95%, perhaps about at least 98%, perhaps even about 100% sequence identity.

Nucleic acid of the present invention can also be by methods known in the art synthetic wholly or in part.Therefore all or part of codon of choosing host's preference then that can be to use of nucleic acid of the present invention comes synthetic.The codon of species preference can pass through, and for example, codon the most used from expressed proteins the specific host species is determined.Can obtain having the active mutant of slight change to other modification of nucleotide sequence.

DsRNA or siRNA nucleotide sequence comprise the two strands of polymeric ribonucleotide, and can comprise the modification to phosphoric acid-sugar backbone or nucleosides.The modification of RNA structure can be adjusted, and suppresses to allow specificity heredity.In one embodiment, can modify the dsRNA molecule by enzyme method, thereby can produce the siRNA molecule.SiRNA can effectively mediate the downward modulation effect at some target genes in some insects.This kind of enzyme method can be finished (people such as Elbashir, 2002 by utilize the RNAse III enzyme or the DICER enzyme that exist in insect, vertebrates, fungi or the vegetable cell in eucaryotic RNA i approach; Hamilton and Baulcombe, 1999).This method can utilize also that those skilled in the art understand easily passes through reorganization DICER or RNAse III that recombinant DNA technology is incorporated in the target insect cell and carries out.Naturally be present in the dsRNA chain that DICER enzyme in the insect or by the recombinant DNA technology manufacturing and RNAse III can will be bigger and be cut into less oligonucleotide.The DICER enzyme can be cut into the siRNA fragment with the dsRNA molecule specifically, and wherein, the segmental length of each siRNA is about 19-25 Nucleotide, and the RNAseIII enzyme is cut into the dsRNA molecule siRNA of 12-15 base pair usually.3 ' the overhang and 5 ' phosphoric acid and the 3 ' C-terminal that have 2 to 3 Nucleotide by the siRNA molecule of above-mentioned any enzyme generation.The siRNA that produces by RNAse III enzyme is with identical by those of Dicer enzyme generation in eucaryotic RNA i approach, therefore, then can be fixed by target, and after loose subsequently, degraded by intrinsic cell RNA degradation mechanism, be separated into single stranded RNA, and the RNA sequence hybridization of transcribing with target gene.This method causes the nucleotide sequence coded RNA sequence of target gene in the insect is effectively degraded or removed.The result is exactly by the silence of the fixed nucleotide sequence of special target in the insect.The details of enzyme method is described and can be found in Hannon (2002).

Nucleotide sequence of the present invention can be recorded on the computer-readable medium." computer-readable medium " used herein refers to: can be by the tangible expression medium of computer direct reading and access.This type of medium includes but not limited to: magnetic-based storage media, floppy disk for example, hard disk, storage media and tape; Optical storage medium, for example CD-ROM; Electronic storage medium, for example RAM and ROM; The computer document of optical character recognition form, and the heterozygote of mentioned kind, for example, magnetic/optical storage medium.It will be readily apparent to those skilled in the art that any known computer-readable medium can be used to make following product at present, described product comprises the computer-readable medium that writes down nucleotide sequence of the present invention.

" record " used herein refers to, is used for the method for storage information on computer-readable medium.Those skilled in the art can easily adopt any present known method to come recorded information on computer-readable medium, comprise the medium of nucleotide sequence information of the present invention with generation.To those skilled in the art, multiple different data store organisation is obtainable, is used to make the computer-readable medium of minute book invention nucleotide sequence.Selection to the data storage media usually will be based on selecting for use access to be stored the means of information.In addition, multiple different data processor program and form can be used for nucleotide sequence of the present invention is stored on the computer-readable medium.Sequence information can be showed in the word processing text document, with the software that can obtain by commercial sources, the form of WordPerfect and Microsoft Word for example, perhaps it can be shown as the form of ASCII text document, be stored in the database application, for example DB2, Sybase, Oracle etc.Those skilled in the art can easily adapt any amount of data handler structured format (for example, text document or database), to obtain to record the computer-readable medium of nucleotide sequence information of the present invention.

The computer software of the sequence information that provides in permission those skilled in the art accessing computer readable medium is that the public is obtainable.In the Sybase system, carry out BLAST (people such as Altschul, 1990) and BLAZE (people such as Brutlag, 1993) software of searching algorithm can be used to identify the reading frame (ORF) in the sequence, for example, provided herein, contain and from the Unigene and the EST of other biological ORF or proteic homology.This type of ORF is the fragment of coded protein in the sequence of the present invention, can be used for producing commercial important protein, for example, be used for that amino acid bio is synthetic, metabolism, transcribe, the enzyme of translation, RNA processing, nucleic acid and proteolytic degradation, protein modification and dna replication dna, restriction, modification, reorganization and reparation.

The present invention also provides the system that contains sequence information as herein described, particularly the computer based system.This type systematic is designed to: can identify the commercially important fragment in the nucleic acid molecule of the present invention." computer based system " used herein refers to be used to analyze hardware device, software equipment and the data storage device of Nucleotide information of the present invention.The minimal hardware equipment of computer based of the present invention system comprises central processor unit (CPU), input unit, output equipment and data storage device.Those skilled in the art can easily instruct, and anyly in the present obtainable computer based system all are suitable for the present invention.

" target structure motif " used herein or " target motif " refer to, any sequence or the combined sequence rationally selected, and wherein, the 3-d modelling that forms when folding based on target motif is selected one or more sequences.Multiple target motif known in the art.The protein targets motif includes but not limited to, enzyme active sites and signal sequence.The nucleic acid target motif includes but not limited to, promoter sequence, cis-acting elements, hairpin structure and inducible expression element (protein binding sequence).

B. recombinant vectors and host cell transform

Recombinant DNA carrier can be a cyclic plasmid for example linear or sealing.Carrier system can be single carrier or plasmid, or two or more carriers or plasmid, and they contain the total DNA that will be introduced in the host bacterium genome together.In addition, bacteria carrier can be an expression vector.Nucleic acid molecule shown in the SEQ ID NO:1-SEQ ID NO:906 in the sequence table or its fragment or its complementary sequence can, for example, be inserted in the carrier suitably, described carrier is under the control of suitable promotor, described promotor plays a role in one or more microorganism host, to drive the encoding sequence connected or the expression of other dna sequence dna.For this purpose, a lot of carriers all are obtainable, will depend primarily on the size of the nucleic acid that will be inserted in the carrier and the particular host cell that will transform with carrier to the selection of suitable carrier.Every kind of carrier all contains various ingredients, and this depends on its function (DNA amplification or expressible dna) and the particular host cell compatible with it.Being used for carrier component that bacterium transforms generally includes but is not limited to one or more of following component: signal sequence, replication orgin, one or more selectable marker genes and the inducible promoter that allows foreign DNA to express.

Expression and cloning vector contain the selection gene usually, and it is also referred to as selective marker.The transformed host cells survival that this genes encoding is grown on selective medium or the necessary albumen of growing.The typical following albumen of genes encoding of selecting, described albumen: (a) give to microbiotic or other toxin, for example, the resistance of penbritin, Xin Meisu, methotrexate or tsiklomitsin, (b) supply auxotrophy, or (c) provide the crucial nutrition that can't from complex medium, obtain, and for example, the gene of coding genus bacillus D-alanine racemase.These can be produced the albumen of giving drug resistance by heterologous protein or its fragment success cell transformed, can survive in selection scheme thus.

The expression vector that is used to produce mRNA also can contain following inducible promoter, described promotor can be by the host bacteria bio-identification, and be operably connected with following nucleic acid, described nucleic acid for example, encoding D .v.virgifera mRNA or its interested segmental nucleic acid.The inducible promoter that is suitable for host bacterium comprises the β-Nei Xiananmei promotor, intestinal bacteria lambda particles phage PL and PR promotor, and intestinal bacteria semi-lactosi promotor, pectinose promotor, alkaline phosphatase promoter, tryptophane (trp) promotor and lactose operon promotor and variant and hybrid promoters, for example, tac promotor.But other known bacteria-induction type promotor also is suitable.

Mentioning when regulating sequence and structure nucleotide sequence, term " is operably connected " and refers to, regulates the expression of being regulated that sequence causes the structure nucleotide sequence that links to each other." adjusting sequence " or " controlling elements " refer to be positioned at the nucleotide sequence of structure nucleotide sequence upstream (5 ' non-coding sequence), inside or downstream (3 ' non-translated sequence), and it can have influence on opportunity and the level or the quantity of the transcribing of dependency structure nucleotide sequence, RNA processing or stability or translation.Regulate sequence and can comprise promotor, translation leader sequence, intron, enhanser, stem-ring structure, repressor binding sequence and poly-adenosine recognition sequence etc.

Perhaps, expression construct can be integrated into bacterial genomes with integrative vector.Typically, integrative vector contains and at least a sequence of bacterial chromosome homologous that allows vector integration.Integration looks like that in carrier DNA and the bacterial chromosome reorganization between the homologous dna causes.For example, use the integrative vector that makes up from the DNA of multiple Bacillus strain can be integrated into genus bacillus karyomit(e) (EP 0,127,328).Integrative vector also can comprise phage or transposon sequence.Suicide vector also is known in the art.

Structure to the suitable carrier that contains one or more components of listing above utilizes the recombinant DNA technology of standard to carry out.To being cut, shear, and be connected to the required plasmid desirable form of generation again through isolating plasmid or dna fragmentation.The example of obtainable bacterial expression vector includes but not limited to, multi-functional escherichia coli cloning and expression vector, for example, Bluescript ^TM(Stratagene, La Jolla, CA), wherein, for example, D.v.virgifera albumen or its fragment can be connected into carrier, described connection be with meet be used for beta-galactosidase enzymes N-terminal Met and subsequently the mode of the reading frame of seven residues carry out, produce hybrid protein thus; PIN carrier (Van Heeke and Schuster, 1989) etc.

Typically, the yeast recombinant precursor comprises one or more in the following component: promoter sequence, fusion partner sequence, leader sequence, transcription termination sequence, selective marker.These elements can be combined into expression cassette, and expression cassette can be held in the replicon, extra-chromosomal element (for example, plasmid) for example, and it can be stablized and remains among the host (for example yeast or bacterium).Replicon can have two cover dubbing systems, allows it for example to be held in thus, is used in the yeast expressing, and remains in and be used for clone and amplification among the prokaryotic organism host.The example of this type of yeast-bacterium shuttle vectors comprises YEp24 (people such as Botstein, 1979), pCl/1 (people such as Brake, 1984) and YRpl7 (people such as Stinchcomb, 1982).In addition, replicon can be the plasmid of high or low copy number.High copy number plasmid will have about 5 copy numbers to about 200 scopes usually, typically, and about 10 to about 150.It is about at least 10 that the host of containing high copy number plasmid will preferably have, more preferably about at least 20 copies.

Useful yeast initiating sequence can obtain by the gene of enzyme from encoding metabolic pathway.The example of this genoid comprises alcoholdehydrogenase (ADH) (EP 0 284044), enolase, glucokinase, G-6-P isomerase, glyceraldehyde-3-phosphate dehydrogenase (GAP or GAPDH), hexokinase, phosphofructokinase, 3-phosphoglycerate mutase and pyruvate kinase (PyK) (EP0 3215447).The yeast PHO5 gene of coding acid phosphatase also provides useful promoter sequence people such as (, 1983) Myanohara.In addition, natural non-existent synthetic promoter also can be used as Yeast promoter and plays a role.The example of this type of hybrid promoters comprises that the ADH that links to each other with GAP transcriptional activation zone regulates sequence (U.S. Patent number 4,876,197 and 4,880,734).The terminator sequence of Transcription Termination subsequence and the identification of other yeast as those example of fgs encoder glycolytic ferment, is well known by persons skilled in the art.

Perhaps, expression construct can be integrated into the yeast genes group with integrative vector.Typically, integrative vector contains and at least a sequence of yeast chromosomal homologous that allows vector integration, and preferably contains the two kind homologous sequences of flank in the table construct.Integration looks like (people such as Orr-Weaver, 1983) that the reorganization between the homologous dna causes in carrier and the yeast chromosomal.Integrative vector can be at the specific gene seat in the yeast, and this is by selecting suitable homologous sequence to comprise that into carrier is realized.People's such as described Orr-Weaver document sees above.One or more expression construct can be integrated, and may influence the level (people such as Rine, 1983) of the recombinant protein of generation.

The present invention also considers nucleotide sequence of the present invention is transformed into plant, makes one or more dsRNA molecular energies suppress horizontal expression with insect.Use the obtainable method in this area, can easily prepare conversion carrier.Conversion carrier comprises one or more following nucleotide sequence, described sequence can be transcribed into the RNA molecule, and remarkable homology of one or more nucleotide sequences and/or complementation with the insect genes group coding, when making picked-up RNA, can reduce at least a expression in each nucleotide sequence in the insect genes group.

Conversion carrier also can be called the dsDNA construct, and it also can be defined as recombinant molecule, insect-controlling agent, genetic molecule or mosaic heredity construct.Mosaic heredity construct of the present invention can comprise, for example, encode one or more antisense transcripts, one or more have the nucleotide sequence of adopted transcript, above-mentioned one or more materials, wherein, from its all or part of homology of all or part of and following RNA molecule of transcript, described RNA molecule comprises RNA sequence nucleotide sequence coded in the insect genes group.

In one embodiment, plant conversion carrier comprises the dna molecular of separation and purifying, wherein comprises the promotor that operationally links to each other with one or more nucleotide sequences of the present invention.Nucleotide sequence is selected from the SEQ ID NO:1-SEQ ID NO:906 in the sequence table.Nucleotide sequence comprises following fragment, and the RNA's that exists in its coding target insect rna transcription thing is all or part of, also comprises all or part of inverted repeats of target insect RNA.The dna molecular that comprises expression vector also can contain functional intron sequences (its be positioned the upstream of encoding sequence or even among encoding sequence), described molecule can also contain five casts aside (5 ') untranslated leader (promptly, UTR or 5 '-UTR), it is positioned between promotor and the translation initiation site.

Plant conversion carrier can contain the sequence from more than one genes, allows more than one dsRNA to produce thus, is used for suppressing two or more expression of gene of target insect cell.One of ordinary skill in the art will readily recognize that the dna fragmentation one-tenth unitary composite capable of being combined dna fragmentation that has corresponding to the sequence of the sequence that exists in the different genes, be used for expressing transgenic plant.Perhaps, can be modified the plasmid of the present invention that contains at least a dna fragmentation, this is undertaken by extra dna fragmentation is inserted between enhanser and promotor and the terminator sequence in proper order.Be used for suppressing in the insect-controlling agent of the present invention of several genes in design, treat that suppressor gene can obtain from same insect species, to strengthen the efficient of insect-controlling agent.In some embodiments, gene can obtain from different insects, to widen effective insect scope of reagent.When in order to prevent or at the combination of expressing and preventing and target when deciding several genes, can be according to Fillatti, application publication number US 2004-0029283 elaboration is made polycistron DNA element with disclosed.

The promotor that has function in different plant species also is well known in the art.Be used in that the promotor of express polypeptide comprises in the plant, the described induction type of people such as Odell (1985), virus, synthetic or constitutive promoter, and/or by the promotor of regulating from time adjusted, space adjusted and space-time.Preferred promotor comprises enhanced CaMV35S promotor and FMV35S promotor.With regard to purpose of the present invention, for example, for the optimum control of the species of ingesting at root, the highest level that preferably obtains these genes in plant root is expressed.A large amount of root strengthens promotors to be identified, their be known in the art (people such as Lu, 2000; U.S. Patent number 5,837,848 and 6,489,542).

Typically, recombinant DNA carrier of the present invention or construct will comprise selective marker, and it can give the selection phenotype on vegetable cell.Selective marker also can be used for selecting plant or the vegetable cell that contains code book invention polypeptide or proteic exogenous nucleic acid.Mark can encode biocide resistance, antibiotics resistance (for example, kantlex, G418 bleomycin, Totomycin etc.) or Herbicid resistant (for example, glyphosate etc.).The example of selective marker includes but not limited to that the neo gene of coding kantlex mycin resistance can use kantlex, G418 etc. to select; The bar gene of coding bialaphos resistance; The sudden change epsp synthase gene, its glyphosate resistance of encoding; Nitrilase gene, it gives the resistance to bromoxynil; Mutant acetolactate synthase gene (ALS), it gives the resistance to imidazolone or sulphur urea; And the DHFR gene of anti-Rheumatrex.The described example that is selected from mark is illustrated in United States Patent (USP) 5,550,318; 5,633,435; 5,780,708 and 6,118,047.

Recombinant vectors of the present invention or construct also can comprise selection markers.Selection markers can be used to monitor and express.Exemplary selection markers comprises, β-Pu Taotanggansuanmei or uidA gene (GUS), its known enzyme (Jefferson, 1987 that multiple chromophoric substrate is arranged of encoding; People such as Jefferson, 1987); R-locus gene, the product (people such as Dellaporta, 1988) that its coding can be regulated the generation of anthocyania pigment in the plant tissue (redness); β-Nei Xiananmei gene (people such as Sutcliffe, 1978), the known enzyme that multiple chromophoric substrate (for example, PADAC, color development cynnematin) arranged of this genes encoding; Luciferase genes (people such as Ow, 1986); XylE gene people such as (, 1983) Zukowsky, its coding catechol dioxygenase, described enzyme can transform the catechol of color development; Alpha-amylase gene (people such as Ikatu, 1990); Tyrosinase cdna (people such as Katz, 1983), its coding can be oxidized to tyrosine the enzyme of DOPA and DOPA quinone, and the DOPA quinone is a melanochrome with after; Alpha-galactosidase, the α-semi-lactosi substrate of its energy catalysis color development.

Preferred plant conversion carrier comprise Ti-plasmids from Agrobacterium tumefaciens (for example, U.S. Patent number 4,536,475,4,693,977,4,886,937,5,501,967 and EP 0 122 791).Rhizobiaceae plasmid (or " Ri ") also can use, and they are known in the art.Other preferred plant conversion carrier comprise Herrera-Estrella (1983), Bevan (1983), Klee (1985) and EP 0 120516 disclosed those.

Usually, preferably in Plant Genome non-specific position introduce functional recombinant DNA.Under special circumstances, can insert the recombinant DNA construction body by site-directed integration.Exist some known can making to implant the multiple fixed point recombination system with function, it comprises United States Patent (USP) 4,959,317 disclosed cre-lox and United States Patent (USP) 5,527,695 disclosed FLP-FRT.

The appropriate method that is used for transformed host cell of the present invention comprises the almost any method that DNA can be introduced cell, for example directly sending of DNA passed, (the people such as Omirulleh of the protoplast transformation by PEG mediation for example, 1993), the DNA of drying/inhibition mediation picked-up (people such as Potrykus, 1985), electroporation (U.S. Patent number 5,384,253), stir (people such as Kaeppler, 1990 with silicon carbide fiber; U.S. Patent number 5,302,523; With U.S. Patent number 5,464,765), agrobacterium-mediated conversion (U.S. Patent number 5,591,616 and U.S. Patent number 5,563,055) and the particle of DNA bag quilt quicken (U.S. Patent number 5,550,318; U.S. Patent number 5,538,877; With U.S. Patent number 5,538,880) etc.By such as above-mentioned technology, can stable conversion the cell of any species almost.Under the situation of many cells species, can render transgenic cell regeneration be genetically modified organism.

Set up transgenic plant, particularly the method for expressing heterologous nucleic acid is known in plant, and can be with nucleic acids for preparation transgenic plant provided herein, and it is to target insect biology, reduces as the susceptibility of ingesting of corn rootworm.For example, can insert plant conversion carrier by nucleic acid and be introduced into plant, can prepare plant conversion carrier generation dsRNA disclosed herein.By modifying the natural gene transfer system of Agrobacterium tumefaciens, obtained a kind of known carrier system.The natural series turnkey contains big Ti (tumor inducing) plasmid, and it contains the big fragment that is called T-DNA, and it is transferred in the plant transformed.Another fragment of Ti-plasmids, promptly the vir district is responsible for T-DNA and shifts.The border in T-DNA district is a terminal repeat.In the binary vector of modifying, removed tumor inducting gene, and utilized the function in vir district to shift the foreign DNA of border for the T-DNA border sequence.The T district also can contain selective marker and a plurality of cloning site that is useful on efficient recovery transgenic plant and cell, and described site is used to insert some sequences, and described sequence is used for jump routine such as dsRNA coding nucleic acid.

Typically, the transgenic plant of using agrobacterium transformation method to form contain the single simple recombinant DNA sequence that is inserted in the karyomit(e), and it is called as transgenic event.It is heterozygosis that these type of transgenic plant can be called as for the exogenous array that inserts.The transgenic plant of isozygotying for the transgenosis body can produce the F1 seed and obtain by the transgenic plant selfing that makes independent separate.In the F1 seed that produces 1/4th will isozygoty for the transgenosis body.Make the F1 seed germination, just having produced can tested heterozygosity or the plant of homozygosity, typically, uses SNP to measure or hot amplification assay carries out, and described mensuration allows the difference (that is zygosity determination) between heterozygote and the homozygote.

C. expression of nucleic acid and target gene are prevented

As host transformed or or the example of symbiote insect target biology, the present invention also provides plant transformed cell and plant transformed and their offspring.Plant transformed cell and plant transformed can through engineering approaches, to express one or more dsRNA disclosed herein or siRNA sequence, so that the insect protective effect to be provided.These sequences can be used for the gene of insect biology and prevent, thereby reduce the invasion and attack of insect on shielded conversion host or the symbiote biology.Vocabulary used herein " gene is prevented " is used for representing being used to reduce any known method that genetic transcription becomes the level of mRNA and/or mRNA translation subsequently.

Gene is prevented the reduction that also is used to refer to from the protein expression of gene or encoding sequence, comprises that posttranscriptional gene is prevented and transcription repression.Posttranscriptional gene prevents all or part of that be that gene that from preventing target is fixed or encoding sequence transcribe the mRNA that comes to mediate with homology between the corresponding double-stranded RNA that is used to prevent, and it refers to the reduction that the obtainable amount that is used for rrna bonded mRNA maybe can measure significantly in cell.The RNA that process is transcribed can be a sense orientation, brings into play so-called resistance inhibitor action altogether, can be antisense orientation perhaps, brings into play so-called antisense resistance inhibitor action, perhaps produces dsRNA with two kinds of directions, brings into play so-called RNA interference effect (RNAi).

Transcription repression is that the existence of dsRNA gene co-inhibitor in the cell mediates, and described dsRNA gene co-inhibitor shows the remarkable sequence identity with promoter DNA sequence or its complementary sequence, and performance is called as the effect that promotor oppositely suppresses.Gene is prevented and can be played a role at the natural plant gene relevant with proterties, for example, to plant provide have the reduction level, by the natural gene encoded protein, perhaps having increases or the affected metabolite of reduction level.Gene is prevented also and can be played a role at the target gene of following plant insect, described insect can absorb or contact the vegetable material that contains the gene co-inhibitor, and described gene co-inhibitor is specifically designed the expression that can suppress or prevent one or more homologous sequences in the insect cell or complementary sequence.U.S. Patent number 5,107 discloses antisense in 065,5,759,829,5,283,184 and 5,231,020 or has had the directed RNA of justice to carry out posttranscriptional gene and prevented, to regulate the genetic expression in the vegetable cell.DsRNA is used for preventing the purposes of plant gene to be disclosed WO 99/53050, WO 99/49029, U.S. Patent Application Publication No. 2003/0175965 and 2003/0061626, Application No. 10/465,800 and U.S. Patent number 6,506,559 and 6, in 326,193.

In plant, carry out useful method that posttranscriptional gene prevents and use the directed and RNA that the transcribes antisense orientation, stabilization of justice is arranged, for example, as hair clip and loop-stem structure.Be used to carry out preferred DNA construct that posttranscriptional gene prevents and be following like this, wherein, first section fragment coding show antisense orientation RNA (its show and target fragment to be prevented between remarkable identity), described first section fragment links to each other with second section fragment, and second section fragment coding shows with first section fragment has highly complementary RNA.This type of construct estimated to form loop-stem structure (this forms with second section fragment hybridization by first section fragment) with from the ring structure (seeing WO94/01550, WO98/05770, US2002/0048814 and US 2003/0018993) that is connected both section segmental nucleotide sequences.

According to another embodiment of the invention, a kind of nucleotide sequence is provided, its vivoexpression causes transcribing of stabilization RNA sequence, the remarkable homology of RNA molecule of target gene in described RNA sequence and the insect, and described RNA molecule comprises the nucleotide sequence coded RNA sequence in the insect genes group.Therefore, after the stabilization RNA sequence of mixing in the insect pickuping food or plant surface sprays, will cause in the target insect cell nucleotide sequence to be reduced corresponding to target gene.

It is sequence-specific utilizing stabilization dsRNA technology of the present invention to suppress target gene because, with the duplex of RNA zone corresponding nucleotide sequences be fixed by target for heredity suppresses.The RNA that contains the nucleotide sequence identical with the part of target gene is optimized for inhibition.The RNA sequence that relative target sequence has insertion, disappearance and simple point mutation also is found and can effectively suppresses.At aspect of performance of the present invention, preferably, the part of inhibition dsRNA and target gene is shared about at least sequence identity more than 80%, perhaps about sequence identity more than 90%, perhaps about sequence identity more than 95%, perhaps about sequence identity more than 99%, perhaps even about 100% sequence identity.Perhaps, the duplex zone of RNA can functionally be defined as: can with the nucleotide sequence of the part of target gene transcript hybridization.But show the long sequence that less homology is but only arranged of the sequence compensate for slower less than total length of higher homology.The length of identical nucleotide sequence can be about at least 25,50,100,200,300,400,500 or about at least 1000 bases.Usually, should use the sequence above 20-100 Nucleotide, but the sequence that surpasses about 200-300 Nucleotide being preferred, is especially preferred above the sequence of about 500-1000 Nucleotide, and this depends on the size of target gene.The present invention has following advantage: it can tolerate sequence variations, and described variation may cause owing to genetic mutation, bacterial strain polymorphism or evolutionary divergence.With respect to the primary transcription product of target gene or through the mRNA of processing fully, the nucleic acid molecule of introducing may not need absolute homology, may need not be total length.Therefore, those skilled in the art need recognize that as disclosed herein, 100% sequence identity between RNA and the target gene is also nonessential for implementing the present invention.

Can be by quantitatively to the inhibition of expression of target gene, this is to be undertaken by measuring the albumen that endogenous target RNA or target RNA translation produced, the result of inhibition can verify by the inspection of pair cell or biological extrinsic property.The method that is used for RNA and protein quantification is well known to a person skilled in the art.The multiple choices mark of giving the resistance of penbritin, bleomycin, paraxin, gentamicin, Totomycin, kantlex, lincomycin, methotrexate, careless fourth phosphine, tetracycline, spectinomycin, Rifampin and tsiklomitsin etc. is obtainable.

In some embodiments, genetic expression has been suppressed at least 10%, preferably, and at least 33%, more preferably, at least 50%, further more preferably, at least 80%.In particularly preferred embodiment of the present invention, the genetic expression in the insect cell has been suppressed at least 80%, more preferably, and at least 90%, more preferably, at least 95%, perhaps at least 99%, make significant the inhibition take place.Significant suppress to be used to refer to enough inhibition, its phenotype that causes to be detected (for example, larval growth stops, paralysis or death etc.) or pairing RNA of repressed target gene and/or proteinic can detectedly the minimizing.Though In some embodiments of the present invention, be suppressed in basic all cells of insect and take place, other preferred embodiment in, suppress only in the cell subgroup of expressing this gene, to take place.For example, if the gene that will suppress plays a significant role in the gastral cell of insect, intragentic inhibition just is enough to insect is applied deleterious effects to these cells.

The dsRNA molecule can interior or external synthesizing of body.DsRNA can be by wall scroll self complementary RNA chain formation, perhaps can be by two complementary RNA chain formation.The endogenous RNA polysaccharase of cell can mediate in the body transcribes, and perhaps Ke Long RNA polymkeric substance can be used in the body or in-vitro transcription.Can come target to suppress surely by following method: the specific transcriptional in organ, tissue or cell type; The stimulation of envrionment conditions (for example, infect, coerce, temperature, chemical inducer); And/or transcribe in the through engineering approaches that etap or age carry out.The RNA chain can yes or no poly-adenosineization; The RNA chain can or can not be translated as polypeptide by the translating equipment of cell.

RNA of the present invention, dsRNA, siRNA or miRNA are produced by chemical process or enzyme method by those skilled in the art, and this is by manually or automatically reacting or carrying out in another organism.Also can make RNA by organic synthesis partially or completely; Can synthesize or any ribonucleotide of organic synthesis introducing by vitro enzyme through modifying.Can synthesize RNA by intracellular rna polysaccharase or phage rna polymerase (for example, T3, T7, SP6).Expression construct use and manufacturing is (sees, for example, WO 97/32016, U.S. Patent number 5,593,874,5,698,425,5,712,135,5,789,214 and 5,804,693) known in the art.If by chemosynthesis or synthetic, can before introducing cell, carry out purifying to RNA by vitro enzyme.For example, can from mixture, be purified into RNA by with solvent or resin extraction, precipitation, electrophoresis, chromatogram or its combination.Perhaps, can or only carry out at purifying not using RNA under the situation of minimal purifying, to avoid because the loss that sample processing causes.RNA can be dried to store, and perhaps is dissolved in the aqueous solution.Solution can contain buffer reagent and salt, with the annealing that promotes the duplex chain and/or stable.

For transcribing from transgenosis body or expression construct in vivo, can use regulation domain (for example, promotor, enhanser, silencer and poly-adenosineization) to transcribe (or many) RNA chain.Therefore, in one embodiment, the nucleotide sequence that is used for making the RNA molecule can operationally link to each other with the one or more promoter sequences that have function at microorganism, fungi or plant host cell.Ideally, nucleotide sequence is placed under the control of endogenesis promoter, and described endogenesis promoter is in the host genome usually.Be in the nucleotide sequence of the present invention under the promoter sequence control that is operably connected, flank can also have extra sequence, and described extra sequence can advantageously influence the stability of its transcript of transcribing and/or obtaining.This type of sequence is usually located at the upstream of the promotor that is operably connected, and/or the downstream of expression construct 3 ' end, can be present in the downstream of promotor upstream and expression construct 3 ' end simultaneously, but also considers only to have described upstream sequence.

" insect-controlling agent " used herein or " gene co-inhibitor " refers to specific RNA molecule, it comprises first RNA fragment and second RNA fragment, wherein the complementarity between first and second the RNA fragment cause two fragments in vivo or external hybridization form the ability of duplex molecule.May preferably include the 3rd RNA fragment usually, its connection and stablize first and second sequence makes entire structure form loop-stem structure, or even the structure of tightr hybridization can form stem ring knotting (knotted) structure.Perhaps, can wherein not have the ring of design, but for steric reason, enough stablize himself when the length of stem not having to form symmetrical hair clip under the 3rd the segmental condition, hair clip will be set up the ring of himself.First and second RNA fragments are usually located in the length of RNA molecule, and are significantly oppositely repetition each other, and are linked together by the 3rd RNA fragment.First and second fragments always and be not respectively corresponding to target RNA justice and antisense sequences arranged, the target gene that described target RNA is the target insect pest transcribes, described target gene can be prevented by the picked-up of dsRNA molecule.Insect-controlling agent can also be the nucleic acid molecule of highly purified (or separation), more particularly, and from nucleic acid molecule or its nucleic acid fragment molecule in genomic dna (gDNA) or cDNA library.Perhaps, described fragment can comprise littler oligonucleotide, and it has about 15 to about 250 nucleotide residues, and more preferably, about 15 to about 30 nucleotide residues.

Term used herein " genome " not only comprises the chromosomal DNA of finding in the nuclear when being used for insect or host cell, it also is included in the organelle DNA of finding in the subcellular components of cell.Therefore, DNA of the present invention is introduced in process in the vegetable cell and can locatees by chromosomal integration or by organoid and carry out.Term " genome " comprises karyomit(e) and the plasmid in the bacterial host cell when being applied to bacterium.Therefore, DNA of the present invention is introduced in process in the bacterial host cell and can locatees by chromosomal integration or by plasmid and carry out.

Term used herein " insect " refers to insect, spider, Crustacean, fungi, bacterium, virus, nematode, flatworm, roundworm, pinworm, hookworm, tapeworm, taper worm, schistosomicide, horse botfly, flea, flat lice, mite, lice etc., they are that nature is ubiquitous, one or more can be absorbed or contact by cell, tissue or fluid that insect host or symbiote (be converted into and can express or wrap by double-stranded gene co-inhibitor) produce, perhaps the vegetable material that contains the gene co-inhibitor can be absorbed." pest resistance " used herein is the feature of transgenic plant, transgenic animal, transformed host or transgenosis symbiote, it makes described plant, animal, host or symbiote have resistance for the attack of insect, and described attack typically can cause damage or loses described plant, animal, host or symbiote.This type of pest resistance may produce from natural sudden change, perhaps more typically, produces mixing from the recombinant DNA of giving pest resistance.For the render transgenic plant has insect-resistant, recombinant DNA can for example be transcribed into the RNA molecule, forms the dsRNA molecule in the tissue of recombinant plant or fluid.The dsRNA molecular moiety comprises: with the identical RNA fragment of corresponding RNA fragment of dna sequence encoding in the harmful insect that preference is ingested on recombinant plant.Expression of gene is prevented by dsRNA in the target insect pest, causes plant to have insect-resistant to preventing of genetic expression in the target insect pest.People such as Fire (U.S. Patent number 6,506,599) have described the inhibition to the insect invasion and attack prevailingly, only provide details at the some middle nucleotide sequence that has the suppressor gene function in nematode kind caenorhabditis elegant.Similarly, people (US 2003/0061626) such as Plaetinck has described dsRNA and has been used for purposes in multiple nematode pests kind suppressor gene function.People such as Mesa (US 2003/0150017) have described: use dsRNA sequence transformed host cell, with the essentially identical corresponding dsRNA sequence of target sequence in expression and the special pathogen, and described especially: make up recombinant plant, it expresses these dsRNA sequences (being absorbed by the various plants insect), promotion is to the downward modulation of gene in the insect genome, and the raising plant is to the resistance of insect invasion and attack.

The invention provides the method that the dsRNA that uses stabilization suppresses the genetic expression of one or more target genes in the target insect.The present invention is particularly useful in the adjusting eukaryotic gene expression, particularly regulate the expression of gene that exists in the insect, described insect shows about 4.5 to about 9.5, more preferably about 5.0 to about 8.0, further more preferably about 6.5 to about 7.5 Digestive tract pH.For the plant insect of Digestive tract, may need to adopt the method for passing of sending that does not require picked-up dsRNA molecule with the pH level outside these scopes.

The regulating effect of dsRNA is applicable to the several genes of expressing in insect, for example, be responsible for the native gene of cellular metabolism or cell transformation, comprises other gene of the polypeptide that house-keeping gene, transcription factor and Codocyte metabolism relate to.

Phrase used herein " inhibition of genetic expression " or " suppressing target gene expression in the insect cell " refer to that there be not (or observable reduction) in the albumen of target gene and/or mRNA product level.Specificity refers to: suppress target gene and other gene of pair cell does not have effect and the ability that any gene in the cell that produces the dsRNA molecule not have influence.May cause new phenotypic character in the insect pest to the inhibition of the genetic expression of target gene in the insect pest.

A part of the present invention provides a kind of delivery system that send, and is used for insect-controlling agent sent being delivered to insect, and this is to realize by they being exposed to the food that contains insect-controlling agent of the present invention.According to one of embodiment, the dsRNA of stabilization or siRNA molecule can be impregnated in insect food, perhaps can be coated over the top of the food of insect consumption.A part of the present invention also provides and has been used for sending the delivery system that send that is delivered to insect with insect-controlling agent, this be by: they are exposed to microorganism or the host of containing insect-controlling agent of the present invention, for example, plant, this realizes by ingested microorganisms or host cell or entocyte.In another kind of embodiment, the present invention relates to prepare transgenic plant cells or plant, wherein contain the recombinant DNA construction body that can transcribe stabilization dsRNA molecule of the present invention.Phrase used herein " preparation transgenic plant cells or plant " refers to following method, it comprises and uses the recombinant DNA technology that is easy to obtain in this area (for example, see Sambrook, Deng the people), structure can be transcribed the plant conversion carrier of stabilization dsRNA molecule of the present invention, transformed plant cells or plant, and preparation contains through transgenic plant cells or transgenic plant that transcribe, stabilization dsRNA molecule.

In another embodiment, the attenuated strain of the non-virulent of microorganism can be used as the carrier of insect-controlling agent, and in this case, the microorganism of carrying this type of reagent is also referred to as insect-controlling agent.Can be in addition engineered to microorganism, make its nucleotide sequence of expressing target gene, producing following RNA molecule, described RNA molecule comprise with insect cell in typical case the RNA sequence homology or the complementary RNA sequence that exist.Insect is exposed to described microorganism, makes microorganism be ingested, and by RNA molecule or its fragment or the direct or indirect downward modulation that mediates expression of target gene of derivative.

Perhaps, the invention provides: insect is exposed to insect-controlling agent of the present invention, and described control agent is impregnated in the spray mixing device, is applied to the host, for example, and the surface of host plant.In a kind of exemplary embodiment, insect is to the picked-up of insect-controlling agent, insect-controlling agent sent be delivered to the insect digestive tube, send subsequently to be delivered in the intravital cell of insect.In another embodiment, insect-controlling agent also can allow sending of insect-controlling agent to pass by alternate manner (for example injection or other physical method) to the infection of insect.In another embodiment, RNA molecule self is packed to advance synthetic matrix, polymkeric substance for example, and be applied to the host, for example surface of plant.Insect allows insect-controlling agent to be sent to the picked-up of host cell to be delivered to insect, to cause the downward modulation to target gene among the host.

Also can predict, composition of the present invention can be impregnated in the seed of plant species, and it can be used as the expression product that is impregnated in the genomic recombination of vegetable cell, perhaps is impregnated in the coating or seed treatment that is administered to before the plantation on the seed.The vegetable cell that contains recombination is counted as transgenic event in this article.

Think that insecticidal seed treatment can provide significant advantage when with the transgenic event combination of the provide protection of the invasion and attack that are provided for resisting coleopteran pest, described incident is in the preferred validity scope at the target insect.In addition, we believe, and are as well known to those skilled in the art, have following situation, and wherein, this type of transgenic event that has in preferred validity scope is favourable.

A part of the present invention provides a kind of delivery system that send, and is used for sending to insect passing insect-controlling agent.Stabilization dsRNA of the present invention or siRNA molecule can be introduced directly into the cell of insect, perhaps be incorporated into the recycle system of extracellular chamber, interstitial space, lymphsystem, Digestive tract, insect, this is undertaken by orally ingestible or utilizable other means of those skilled in the art.Be used for comprising by the method for oral introducing: RNA is directly mixed with the food of insect, and following engineering method, wherein, the species that are used as food have passed through engineered, are expressed dsRNA or siRNA, it is fed raise to be influenced insect then.In one embodiment, for example, dsRNA or siRNA molecule can mix in the insect food, or cover its surface.In another embodiment, RNA can be sprayed at plant surface.In another embodiment, can be by microbial expression dsRNA or siRNA, and microorganism can be administered to plant surface, perhaps introduce root, stem as injection by physical means.In another embodiment, can carry out genetically engineeredly, make its expression be enough to kill the dsRNA or the siRNA of the amount of known insect that can infection plant plant.

Especially, in WCR, put into practice when of the present invention, stabilization dsRNA or siRNA can be incorporated into the intravital midgut of insect, obtain the inhibition of ideal target gene.As indicated above, dsRNA or siRNA molecule can be comprised the feed thing or are covered on the food, can be absorbed by insect.Under any circumstance, dsRNA of the present invention is provided in the food of target insect.Target insect of the present invention will show about 4.5 to about 9.5 or about 5 to about 8.5 or about 6 to about 8 or about 6.5 to about 7.7 or about 7.0 digestive tube pH.The digestive tube of target insect is defined as the following position in the insect in this article, in this position, the food that insect absorbed is exposed to the environment that can help absorbing dsRNA molecule of the present invention, thereby and can not meet with extreme to making the dissociate pH of formation single chain molecule of hydrogen bond between the dsRNA two strands.

Also can predict, the available mode consistent with the conventional agriculture practice prepared the dsRNA that produces by chemistry or enzymic synthesis, used as being spray product, with the control insect infestations.Described prescription can comprise: effective blade covers required suitable tackiness agent and wetting agent, and is used to protect dsRNA to avoid the UV protective agent of UV infringement.Examples of such additives is commonly used in the biological insecticide industry, also well known to a person skilled in the art.This type of application can with other insecticide of spraying (yes or no based on biology) application combination, to strengthen plant protection, exempt from insect and ingest.

The present inventor considers that the bacterial isolates of producing insecticidal protein can be used to produce the dsRNA that is used for insect control purpose.These bacterial strains can show the insect control character of raising.Multiple different host bacterium can be used to produce insect control dsRNA.Exemplary bacterium can comprise intestinal bacteria, bacillus thuringiensis, pseudomonas kind, polished rod bacterial classification, Xenorhabdus kind, have a liking for worm Serratia and relevant Serratia kind, Bacillus sphaericus, bacillus cereus, bacillus laterosporus, Japanese beetle genus bacillus, clostridium bifermentans and other clostridium kind, or other forms the gram positive bacterium of spore.In certain embodiments, bacterium can be engineered, is used for Pest Control, as mosquito.

The invention still further relates to and be used for the recombinant DNA construction body of expressing in microorganism.Can use methods known in the art, can transcribe the exogenous nucleic acid that obtains interested RNA and introduce microbial host cell, for example, bacterial cell or fungal cell.

Nucleotide sequence of the present invention can be introduced in the protokaryon and eucaryon host of wide scope, produces stabilization dsRNA or siRNA molecule.Term " microorganism " comprises protokaryon and eukaryotic microorganisms species, for example bacterium, fungi and algae.Fungi comprises yeast and filamentous fungus etc.Exemplary prokaryotic organism, existing Gram-negative also has gram-positive, comprises enterobacteriaceae, for example Escherichia, erwinia, Shigella, salmonella and proteus; Bacillaceae; Rhizobiaceae, for example rhizobium; Spirillaceae, for example photobacteria genus, fermentation monospore Pseudomonas, serratia, Aeromonas, Vibrio, Desulfovibrio, spirillum; Lactobacillaceae; Pseudomonadaceae, for example Rhodopseudomonas and genus acetobacter; Azotobacteraceae, Actinomy cetaceae and Nitrobacteraceae.Eukaryote comprises fungi, for example phycomyces, and ascomycetes, and it comprises yeast, for example, saccharomyces and Schizosaccharomyces; And basidiomycetes, for example, Rhodotorula, Aureobasidium, Sporobolomyces etc.

With regard to the purpose of the anti-insect of protective plant, a large amount of microorganisms of known blade face (surface of plant leaf) that inhabits the important farm crop of multiple difference and/or rhizosphere (soil around the plant root) also can be to be used for recombinant precursor disclosed herein is handled, breeds, preserves, sent and pass and/or the desirable host cell of mutagenesis.These microorganisms comprise bacterium, algae and fungi.Interested especially is microorganism, for example, bacterium, for example following genus: bacillus (comprises following kind and subspecies: bacillus thuringiensis Ku Er Stark HD-I subspecies, bacillus thuringiensis Ku Er Stark HD-73 subspecies, thuringiensis cladosporioides bacillus abrupt inverted sublow specie, those subspecies of bacillus thuringiensis Berlin, bacillus thuringiensis Su Yun gold subspecies, many nests of bacillus thuringiensis subspecies, bacillus thuringiensis pine another name for Sichuan Province subspecies, bacillus thuringiensis A Lai subspecies, bacillus thuringiensis galleria mellonella waxmoth subspecies, Bacillus thuringiensis subsp.aizawai, the inferior malicious subspecies of bacillus thuringiensis, bacillus thuringiensis desinsection subspecies, bacillus thuringiensis tenebrionis subspecies and bacillus thuringiensis San Diego subspecies); Rhodopseudomonas, erwinia, serratia, the white Bordetella of Cray, xanthomonas, streptomyces, rhizobium, Rhodopseudomonas is had a liking for the methyl Pseudomonas, Agrobacterium, genus acetobacter, lactobacillus, genus arthrobacter, Azotobacter, leuconos toc and Alcaligenes; Fungi, particularly yeast, for example, following genus: saccharomyces, Cryptococcus, Crewe Vickers yeast belong, Sporobolomyces, Rhodotorula and Aureobasidium.Interested especially is bacterial species around this type of plant, pseudomonas syringae for example, Pseudomonas fluorescens, serratia marcescens, acetobacter xylinum, Agrobacterium tumefaciens, the red bacterium of class ball, xanthomonas campestris, rhizobium melioti, Alcaligenes eutrophus and Wei Nielande vinelandii; And the yeast species around the plant, for example, rhodothece rubra, gluing rhodotorula, Rhodotorula marina, orange rhodotorula, shallow white Cryptococcus, wandering Cryptococcus, sieve Lun Shi Cryptococcus, the Luo Qian sugar yeast, kind ground sugar yeast, Saccharomyces cerevisiae, red shadow yeast, fragrance shadow yeast, Buddhist ground Crewe Vickers yeast and aureobasidium pullulans.

D. transgenic plant

The invention provides the seed and the plant that have above one or more transgenic events.The combination of described incident is called as " piling up " transgenic event.The described transgenic event that piles up can be the incident at same target insect, and perhaps they can be at different target insects.In one embodiment, seed with ability of expressing nucleic acid provided herein also has the ability of expressing at least a other insecticide, it includes but not limited to following RNA molecule, the sequence of the RNA that expresses in the next comfortable target insect of the sequence of described RNA molecule, when expressing at seed or from the vegetable cell that seed grows, it forms the double-stranded RNA structure, wherein, the target insect has caused preventing rna expression in the target insect cell to the picked-up of one or more cells of plant.

In certain embodiments, have the transgenic event that the herbicide tolerant of providing also is provided for the seed of expressing following dsRNA ability, the sequence of described dsRNA is from the target insect.A useful example of herbicide tolerant gene provides glyphosate, i.e. the resistance of N-(phosphonomethyl) glycine (the isopropyl amine salt form that comprises this type of weedicide).

In the method for the invention; transgenic seed or the expression and combination of from the plant of seed growth, killing the dsRNA of insect amount with some chemistry or albumen pesticide treatments seed or plant; can be used to provide unexpected collaborative advantage, comprise that the transgenic plant that protection obtains avoid the unexpected superior effectiveness that the target insect damages.In specific embodiments; with the sterilant of the about 100gm of every 100kg seed to about 400gm; handle expressing some transgenic seed that can form the construct of dsRNA molecule (its sequence is that one or more sequences of expressing obtain) from corn rootworm, the beyond thought outstanding protection of anti-corn rootworm is provided.In addition, think that this type of makes up also the effectively infringement of anti-other insect of plant of the protection emergency state.Seed of the present invention also is used to reduce the sterilant use cost, because than the situation of not using described method, for obtaining the protection of requirement, can use sterilant still less.In addition, because used sterilant still less, and because its application before plantation, there is not independent land for growing field crops to use, we believe that method of the present invention is therefore all safer for operator and environment, and more cheap than traditional method potentially.

" work in coordination with " expression and comprise the combination acts synergistically of the combination of transgenic event and sterilant for insecticidal activity (or effectiveness).But, this does not represent this type of synergy is restricted to insecticidal activity, they also should comprise following beyond thought advantage, for example, reduction, the distribution of sterilant environment of the field of activity that increases, favourable activity profile (being relevant to type and quantity that infringement reduces), sterilant and application cost reduces, sterilant reduces to the personnel's of production operation and maize planting seed exposure, and other advantage well known by persons skilled in the art.

Can be used for sterilant and the insecticide (comprising) in the composition with method and composition of the present invention combination and use this type of method for compositions can be at for example U.S. Patent number 6 as seed treatment and coating, 551, find in 962, it incorporates this paper in full by reference into.

Though think that seed treatment can be applied to the transgenic seed that is in any physiological status, preferably, seed is in the state of enough lasting stabilities, can not infect induced damage resistive in treating processes.Typically, seed can be from land for growing field crops results, under removing from transgenic plant and with any other non-spermatophyte material separation.Preferably, seed also will be stabilized to following degree biologically, promptly make processing can not cause the biology infringement to seed.In one embodiment, for example, handle may be used on the following corn seed, described seed is gathered in the crops, is cleaned and be dried to water content by weight less than about 15%.In another embodiment, seed can be following seed: it is dried, and then coats water and/or other material, carries out drying more then before with pesticide treatments or during handling.In the restriction of describing, think and in any time between results seed and the sowing seed processing to be administered on the seed.Term used herein " unseeded seed " is used to comprise: be in seed results and any period between ground sowing seed (for the purpose of plant germination and growth).When mentioning unseeded seed by sterilant " processing ", this type of processing is not used in and comprises following operation: wherein, sterilant is applied to soil, but not is administered to seed.For example, with planting seed in, in soil band, " T " band or ditch dug with a plow, use this type of and handle, do not think that the present invention is included.

The combination of sterilant or sterilant can be used " purely ", that is, and and without any dilution or there is other component.But typically, sterilant is administered on the seed with the form of insecticidal formulation.This prescription can contain one or more other components of wanting; it includes but not limited to; liquid diluent is used as the tackiness agent of pesticide substrate, is used for the filler of protection seed under stress conditions and the softening agent that is used to improve coating elasticity, adhesion and/or ductility.

Sterilant of the present invention can be used as the component applied of seed coating to seed.One of embodiment by adding sterilant combination of the present invention can use seed coating process known in the art and composition after improveing.For this class coating process and the equipment of its application is disclosed, for example, U.S. Patent number 5,918 is in 413,5,891,246,5,554,445,5,389,399,5,107,787,5,080,925,4,759,945 and 4,465,017.The seed coating composition is disclosed, and for example U.S. Patent number 5,939, and 356,5,882,713,5,876,739,5,849,320,5,834,447,5,791,084,5,661,103,5,622,003,5,580,544,5,328,942,5,300,127,4,735,015,4,634,587,4,383,391,4,372,080,4,339,456,4,272,417 and 4,245, in 432 grades.

The sterilant that uses in coating is those sterilants as herein described.The amount of the sterilant that uses in the processing to seed will depend on the type of seed category and activeconstituents and change, but described processing will comprise, and the sterilant combination that seed and desinsection are effectively measured contacts.When insect is the target insect, described amount will be the amount of the effective insecticide of desinsection.Insecticidal effective dose used herein represents that the amount of insecticide can be killed the larva or the insect in pupa stage that is in growth, perhaps with continuous decrease or postpone the amount of the infringement that insect pest produces.

Usually, the amount of the sterilant of using to seed in the processing changes in following ranges: for every 100kg seed weight, approximately 100gm is to the pesticide active ingredient of about 2000gm.Preferably, the amount of sterilant is in following ranges: for the activeconstituents of the about 50gm of every 100kg seed to about 1000gm; More preferably, in following ranges: for the activeconstituents of the about 100gm of every 100kg seed to about 600gm; Further more preferably, in following ranges: for the activeconstituents of the about 200gm of every 100kg seed to about 500gm.Perhaps, the amount that has been found that the sterilant of the pesticide active ingredient that surpasses about 60gm for every 100kg seed is preferred, more preferably, surpasses about 80gm (every 100kg seed).

The sterilant that is used for handling must can not suppress seed germination, and should can cause the period to seed or plant damage in target insect life cycle, effectively protects seed and/or plant.Usually, after planting, coating will be effectively about 0 to 120 day.Sterilant of the present invention can be administered on the seed with the form of coating.

Advantage of the present invention can include but not limited to: be easy to dsRNA is introduced insect cell, can use the dsRNA of lower concentration, the stability of dsRNA and the validity that suppresses.Use ability lower concentration, stabilization dsRNA to avoid a lot of shortcomings of antisense interferential.The present invention is not restricted to external use, or be restricted to special sequence composition, the group of specific target gene, the specific part of target gene nucleotide sequence, or specific transgenosis or the specific method of passing of sending, this be with obtainable method known in the art in some, for example, antisense and suppress altogether opposite.In addition, genetic manipulation and the biology of non-classical genetic model in also be feasible.

In to practice of the present invention, can select, harmless with existing of the nucleotide sequence guaranteeing to transcribe from recombinant precursor for non-insect cell.This can be by target corresponding gene has low degree sequence identity in fixed and plant or the vertebrates gene realize.Preferably, sequence identity degree is less than about 80%.More preferably, sequence identity degree is less than about 70%.Most preferably, sequence identity degree is less than about 60%.

Except plant directly being transformed, can hybridize with second kind of plant that lacks this construct by first kind of plant that will have the recombinant DNA construction body and prepare transgenic plant with the recombinant DNA construction body.For example, be used for the recombinant DNA that gene prevents and can be introduced in the first kind of plant strain, described strain transforms easily, generation can with the transgenic plant of the second kind of plant incross, gradually be seeped into the second kind of plant strain will be used for the recombinant DNA that gene prevents.

In practice, the present invention can with other insect control proterties combination in the plant, be used to increase proterties with what obtain to want to the control of insect infestations.With utilizing the insect control proterties combination of different binding modes, the transgenic plant of protecting at insect can be provided, it has outstanding endurance than the plant of using single insect control proterties, reduces because develop the probability that resistance in the field.

In the past many decades, the proteic insect mechanism of killing of bacillus thuringiensis crystal has been carried out extensive studies.Show that only after picked-up albumen, crystallin just can be poisonous to the larva form of insect.In coleoptera larvae, alkaline pH in the insect midgut and proteolytic ferment meeting soluble protein make the deleterious component of insect is discharged thus.These toxic components destroy midgut epithelial cells, cause insect to stop to ingest, and final, cause insect death.Former at this point thereby speech, verified bacillus thuringiensis toxin be from being effectively in the process of handling the various insects insect, and be environmentally safe insecticide.Coleoptera and hemipteran, and most likely, Diptera, lygus bug and other boring formula and suctorial type insect have shown the enteron aisle pH of little acid are so it is inoperative to above-mentioned insect effectively to keep out the Bt toxin of coleoptera larvae.The enteron aisle pH of the little acid of these insects is also believed it is more useful to composition of the present invention, do not desiring to be restricted under the situation of particular theory, seem, the alkaline pH of coleoptera larvae enteron aisle be before attempt show reason (people's U.S. Patent number 6 such as Fire of the trial failure of dsRNA effect, 506,559; People's patent publication No. US2003/0150017 such as Mesa; People such as Rajagopal, 2002; People such as Tabara, 1998).Therefore we believe that dsRNA method disclosed herein should preferentially be used for controlling plant and the composition of Coleoptera, Diptera, Hemiptera, lygus bug, boring formula and suctorial type insect.Method and composition shown in this paper is particularly useful in target and fixs the gene of stating in the insect and prevent, and described insect shows about 4.5 to about 9.5, about 5.0 to about 9.0, about 5.5 to about 8.5, about 6.0 to about 8.0, about 6.5 to about 7.7 or about 6.8 to about 7.6 or about 7.0 enteron aisle pH.But, show about 7.5 to about 11.5, or about 8.0 to about 11.0, or the insect of about 9.0 to about 10.0 enteron aisle pH and other pest species, for example the coleopteron larva also falls within the scope of the present invention.When in larva food, providing when being specific to the dsRNA that suppresses the coleoptera larvae gene with one or more Bt albumen, this is especially correct, when providing with threshold value or the level that is higher than threshold value, Bt albumen can be according to conventional route to this coleoptera larvae toxigenicity.Have the existence of toxic one or more Bt toxin for insect species of the same race, will effectively reduce enteron aisle pH, provide stable environment, to apply their restraining effect to the insect pest target gene at double stranded rna molecule.

Can predict, the combination of some stabilization dsRNA construct and one or more insects control protein gene will produce synergy, can strengthen the insect control phenotype of transgenic plant.When the insect biological assay that utilizes artificial food or whole plants tissue to carry out can be used for determine using dsRNA and insect control albumen for larval mortality or growth inhibiting dose response.Those skilled in the art can be checked the mixture of dsRNA molecule and insect protein in biological assay; to identify active combination; described combination is to have synergitic and be (Tabashnik, 1992) that people want at the application in the insect protective plant.Reported the synergetic property (summarizing referring to people such as Schnepf 1998) of kill insects insect between the different insect control albumen.Can predict, have synergetic property between some dsRNA and between some dsRNA and some insect control albumen.

The invention still further relates to the agricultural-food goods that contain one or more sequences of the present invention, and the agricultural-food goods of making from the recombinant plant that contains one or more nucleotide sequences of the present invention or seed, they as embodiments of the present invention by particularly including.The agricultural-food goods that contain one or more sequences of the present invention include but not limited to; the meal of plant, oil, grain that pulverize or whole or seed; perhaps comprise any meal, the oil of recombinant plant or seed, any food of grain that pulverize or whole, described recombinant plant or seed contain one or more sequences of the present invention.In one or more agricultural-food that this paper comprises or agricultural-food goods, the detection of one or more sequences of the present invention is proved, agricultural-food or agricultural-food goods are made of transgenic plant, described transgenic plant are designed to express one or more nucleotide sequences of the present invention, are used for preventing method to control the purpose of insect infestations by the gene that uses the dsRNA mediation.

D. obtain nucleic acid

The present invention also provides a kind of method, is used to obtain to comprise the nucleic acid of the nucleotide sequence of producing dsRNA or siRNA.In one embodiment, described method comprises: (a) survey cDNA or gDNA library with hybridization probe, described probe comprises all or part of from the nucleotide sequence of the fixed insect of target or its homologue; (b) identify can with the dna clone of hybridization probe hybridization; (c) dna clone of identifying in the separating step (b); And (d) cDNA or the gDNA fragment that is included in isolating clone in the step (c) checked order, wherein, the nucleic acid molecule of order-checking can the transcribe rna nucleotide sequence or the whole or major portion of its homologue.

In another embodiment, the method that is used to obtain nucleic acid fragment (nucleotide sequence that wherein comprises the major portion that is used for production dsRNA of the present invention or siRNA) of the present invention comprises the steps: that (a) synthesizes first and second Oligonucleolide primers, their a part of corresponding with from one of nucleotide sequence of the fixed insect of target; And first and second Oligonucleolide primers that (b) use step (a), the cDNA or the gDNA that exist in the amplification cloning vector insert, and wherein, the nucleic acid molecule of amplification can be transcribed the major portion of dsRNA of the present invention or siRNA.

In to practice of the present invention, target gene can be from corn rootworm (CRW), and for example WCR or SCR obtain, perhaps from causing crop plants to be compromised and any insect species of subsequently the underproduction obtains.It is considered herein that some kinds of standards can be used to select preferred target gene.Described gene is such gene: its protein product has renewal rate fast, makes dsRNA suppress to cause the quick reduction of protein level.In some embodiments, it is favourable selecting for use a small amount of decline of expression level can cause the gene to the deleterious effect of insect.Decide the wide spectrum insect species if think target, will be chosen in the gene of high conservative between these species so.On the contrary, in order to bring specificity, In some embodiments of the present invention, select to contain between various insect species or between insect and other biology, guard the gene in the low zone of degree.In some embodiments, selecting not have the gene of the known homologue of other biology is that people want.

Term used herein " derives from " and refers to, can from specific special source or the special nucleotide sequence that obtains of species, though need not be directly from these special source or species.

In one embodiment, select the gene of in the insect digestive tube, expressing for use.Target fixes on the needs that the gene of expressing in the digestive tube has avoided scattering in insect inside dsRNA.Being used for target gene of the present invention can comprise, for example, and with the remarkable homologous of nucleotide sequence of the known digestive tube expressing gene of the protein ingredient of coding vacuole and plasma membrane proton V-ATP enzyme those (people such as Dow, 1997; Dow, 1999).This protein complexes is unique energy supplier of epithelial cell ion transport, the alkalization of enteric cavity in being responsible for.The V-ATP enzyme is also expressed in Ma Er scytoblastema (Malpighian) pipe, the outside grower (outgrowth) that described Ma Er scytoblastema pipe is the insect hindgut, its with the similar mode of Mammals kidney organ, in fluid balance and detoxifcation, play a role to xenobiontics.In another embodiment, the V-ATP enzyme can be Vha68-2 or its homologue or directly to homologue (for example, SEQ ID NO:821).

The gene of in another embodiment, select main involved in insect growth for use, growing and breeding.Exemplary gene includes but not limited to that CHD3 gene, 'beta '-tubulin gene and coded prediction participate in the proteic gene of transhipment.CHD3 genes encoding in the drosophila melanogaster has the active albumen of ATP dependent form DNA helicase, and assembling is assembled/separated to its chromatin that participates in the nucleus.Similarly sequence also is found in the multiple biology, for example Arabidopis thaliana, caenorhabditis elegant and Saccharomyces cerevisiae.The albumen that 'beta '-tubulin gene family coding is relevant with microtubule, described albumen is the component part of cytoskeleton.Correlated series also is found in the multiple biology, for example caenorhabditis elegant and maduca sexta.Prediction is as the albumen (Babst et al., 2002) of the subunit of the required interior body sorting mixture of transhipment (ESCRT)-III, and for example Dv49 is present in the multiple biology, comprises Mammals, yeast and insect, as D.virgifera.Another kind of transfer related protein is β-coatmer albumen, is abbreviated as β ' Cop, and its coding participates in the product of retrograde (golgi body is to endoplasmic reticulum) transhipment.In caenorhabditis elegant and D.virgifera, all differentiated sequence similar or prediction, as Dv248 (SEQID NO:.

Being used for other target gene of the present invention can comprise, for example, and those that in survival, growth, growth, breeding and infectivity, play an important role.These target genes can be one of the following: the lethality in house-keeping gene, transcription factor and insect specific gene or the fruit bat knocks out sudden change.The target gene of Shi Yonging can also be from those of other biology in the present invention, for example, and from nematode (for example caenorhabditis elegant).In addition, being used for nucleotide sequence of the present invention also can be from plant, virus, bacterium or fungal gene, and the function of described gene has been established in document, and target gene has remarkable similarity in its nucleotide sequence and the insect genes group.According to an aspect of the present invention, for WCR control, target sequence can obtain from target WCR insect substantially.Coding is segmental with known protein homologous D.v.virgifera albumen or its, can find from sequence table from some exemplary target sequences in WCR cDNA library.The nucleic acid molecule from D.virgifera of homologue of coding known protein is known people such as (, U.S. Patent Application Serial 10/205,189) Andersen.

With regard to purpose of the present invention, dsRNA or siRNA molecule can obtain from CRW, and this is by the target CRW gene order or its part that derive from corn rootworm gDNA or cDNA library are carried out polymerase chain (PCR ^TM) amplification realizes.Can prepare the WCR larva with the known method of those of ordinary skills, can extract DNA/RNA.The larva of different sizes can be used for purpose of the present invention from the 1st age to the CRW that grows up to fully, carry out DNA/RNA and extract.The genomic dna or the cDNA library that produce from WCR can be used to PCR ^TMAmplification is to produce dsRNA or siRNA.

Can use the method that obtains easily in this area then, target gene is carried out PCR ^TMAmplification and order-checking.Those skilled in the art can be to PCR ^TMCondition is changed, to guarantee optimum PCR ^TMProduct forms.PCR through checking ^TMProduct can be used as template, is used for transcribing in the body, and what have the minimal promoter of being included with generation has justice and a sense-rna.

The present inventor considers, identifies and isolated nucleic acid sequences can be used for the present invention from any insect species of insect circle, with control WCR and other target insect.In one aspect of the invention, nucleic acid can derive from the Coleoptera species.Especially, nucleic acid can derive from and belong to the chrysomelid of chrysomelid genus (Coleoptera, Chrysomelidae), and more particularly, nucleic acid molecule of the present invention can derive from the virgifera group.The most especially, nucleic acid of the present invention derives from Diabrotica virgifera virgiferaLeConte, and it is commonly called WCR.Can be used for through isolating nucleic acid, for example identify target gene, and make up recombinant vectors, described carrier can be produced stabilization dsRNA of the present invention or siRNA, is used for protective plant and keeps out the WCR insect infestations.

Therefore, in one embodiment, the present invention comprises from the separation of WCR or lygus bug and the nucleotide sequence of purifying, and it can be used as insect-controlling agent.Nucleotide sequence through separation and purifying can comprise those that list in the sequence table.

From WCR or other insect, can be used for nucleic acid of the present invention and also can comprise through separating and highly purified Unigenes and EST nucleic acid molecule or its nucleic acid fragment molecule.The EST nucleic acid molecule can coded polypeptide marking area, perhaps in fact, the major part of polypeptide.Perhaps, described fragment can comprise littler oligonucleotide, and described oligonucleotide has about 15 to about 250 nucleotide residues, and more preferably, about 15 to about 30 nucleotide residues.Perhaps, being used for nucleic acid molecule of the present invention can be from the cDNA library of WCR, lygus bug or any other coleopteran pest species.

Can be used to obtain other nucleic acid molecule from the nucleic acid molecule of WCR or other coleopteran pest species and its fragment, be used for the present invention, dsRNA and the siRNA molecule wanted with generation from other species.This type of nucleic acid molecule comprises following nucleic acid molecule, the proteinic complete encoding sequence of described nucleic acid molecule encoding, and the promotor of this quasi-molecule and flanking sequence.In addition, this type of nucleic acid molecule comprises: encoding gene family member's nucleic acid molecule.Use above-mentioned nucleic acid molecule or its fragment to go to screen for example cDNA or the gDNA library as obtaining, can must obtain this quasi-molecule easily from Lygus hesperus from D.v.virgifera or other Coleoptera species.The method that is used to make this type of library is well known in the art.

Phrase used herein " encoding sequence ", " structure nucleotide sequence " or " structure nucleic acid molecule " refer to, place proper regulation sequence to control following time, can be translated into the nucleotide sequence of polypeptide, and this is undertaken by mRNA usually.The border of encoding sequence is to be determined by 5 ' terminal translation initiation codon and 3 ' terminal translation stop codon.Encoding sequence can include but not limited to, genomic dna, cDNA, EST and recombinant nucleotide sequence.

Term " recombinant DNA " or " recombinant nucleotide sequence " refer to, contain the DNA that genetic engineering is modified, and described modification is to be undertaken by the manipulation of mutagenesis, restriction enzyme etc.

So a lot of in the insect of the potential target controlled by the present invention, the information of the phenotype that obtains about the sequence of most of genes with from the sudden change to specific gene may be limited.Therefore, the inventor considers, select suitable gene from insect pest and be used for process of the present invention, can can be to model animals (for example fruit bat) by using, some other insect species, perhaps in addition the information that the research of corresponding gene obtains in the nematode species, fungal species, plant species (corresponding gene in these species is characterized) carry out.In some cases, may by use from, for example sequence or the gene name of fruit bat, other insect, nematode, fungi or plant (wherein having cloned gene), searching database, GenBank for example obtains the sequence of target insect corresponding gene.In case obtained sequence, can use PCR ^TMIncrease gene fragment in the insect of selecting suitably is used for the present invention.

In order to obtain dna fragmentation, design PCR based on the sequence of in other insect that WCR or gene have been cloned, finding from corresponding gene in the insect species ^TMPrimer.Primer is designed to: the dna fragmentation of the sufficient length that can increase is used for the present invention.DNA (genomic dna or cDNA) preparation is used PCR from insect species ^TMPrimer comes amplification of DNA fragments.Amplification condition is selected to be: even can not accurately mate under the situation of target sequence at primer, amplification also can be carried out.Perhaps, can use WCR gene or other known insect genes, from the gDNA or the cDNA library of insect pest species, clone gene (or its part) from preparation as probe.Be used to implement PCR ^TMWith the technology of from the library, cloning be known.Based on the gene order that clones from WCR or other insect species in the past, the further details of the segmental method of DNA isolation provides in an embodiment to some extent from the target insect pest.Persons of ordinary skill in the art will recognize that multiple different technology can be used for from the insect pest species, isolating with in the past from the corresponding gene fragment of the isolating gene of other species kind.

When insect was target insect of the present invention, this class pest included but not limited to, from lepidopteran (Lepidoptera), for example,

Acleris spp (Acleris spp.), Adoxophyes spp (Adoxophyes spp.), clearwing moth (Aegeria spp.), noctuid (Agrotis spp.), cotton leaf ripple noctuid (Alabamaargillaceae), Amylois spp., Anticarsia (Anticarsia gemmatalis), yellow volume moth (Archips spp), Argyrotaenia spp (Argyrotaenia spp.), Autographa spp (Autographa spp.), Busseola fusca (Busseola fusca), Cadra cautella (Cadra cautella), Carposinanipponensis, striped rice borer (Chilo spp.), Choristoneura spp (Choristoneura spp.), Clysiaambiguella, Cnaphalocrocis spp (Cnaphalocrocis spp.), volume moth (Cnephasia spp.), Cochylisspp (Cochylis spp.), casebearer (Coleophora spp.), Crocidolomia binotalis, Apple abnormity steinernema (Cryptophlebia leucotreta), steinernema (Cydia spp.), Diatraea spp (Diatraea spp.), Diparopsis castanea (Diparopsis castanea), real moth (Eariasspp.), Cadra cautella (Ephestia spp.), Eucosmaspp (Eucosma spp.), Eupoeciliaambiguella, euproctis pseudoconspersa (Euproctis spp.), cutworm (Euxoa spp.), small kernel-eating insect (Grapholita spp.), Hedya nubiferana, noctuid (Heliothis spp.), Hellula undalis (Hellula undalis), fall webworms (Hyphantria cunea), Keiferia lycopersicella (Keiferialycopersicella), pear leaf blister moth (Leucoptera scitella), thin moth (Lithocollethis spp.), Lobesia botrana, poison moth (Lymantria spp.), lyonetid (Lyonetia spp.), tent caterpillar (Malacosoma spp.), lopper worm (Mamestra brassicae), maduca sexta (Manducasexta), geometer (Operophtera spp.), Pyrausta nubilalis (Hubern). (Ostrinia Nubilalis), super steinernema (Pammene spp.), brown volume moth (Pandemis spp.), small noctuid (Panolis flammea), Pectinophora gossypiella (Pectinophora gossypiella), potato tuberworm (Phthorimaeaoperculella), pieris rapae Japan subspecies (Pieris rapae), Fols Picridis fuscipilosae (Pieris spp.), diamond-back moth (Plutella xylostella), ermine moth (Prays spp.), white snout moth's larva (Scirpophaga spp.), pink rice borer (Sesamia spp.), long palpus volume moth (Sparganothis spp.), noctuid (Spodoptera spp.), Synanthedon spp (Synanthedon spp.), band moth (Thaumetopoea spp.), leaf roller (Tortrixspp.), cabbage looper (Trichoplusia ni) and ermine moth (Yponomeuta spp.);

From Coleoptera (Coleoptera), for example,

Wireworm (Agriotes spp.), Anthonomus spp (Anthonomus spp.), Atomaria linearis (Atomaria linearis), Chaetocnema tibialis (Chaetocnema tibialis), weevil (Cosmopolites spp.), fruit resembles (Curculio spp.), khapra beetle (Dermestes spp.), chrysomelid (Diabrotica spp.), Epilachna spp (Epilachna spp.), Eremnus spp., colorado potato beetles (Leptinotarsa decemlineata), rice resembles (Lissorhoptrus spp.), the melolonthid (Melolontha spp.), Orycaephilus spp., Otiorhynchus spp (Otiorhynchus spp.), Phlyctinus spp., Popillia spp (Popillia spp.), flea beetle (Psylliodes spp.), lesser grain borer (Rhizopertha spp.), Scarabeidae (Scarabeidae), rice weevil (Sitophilus spp.), gelechiid (Sitotroga spp.), mealworm (Tenebrio spp.) is modeled one's literary or artistic style on that of the ancients robber (Tribolium spp.) and khapra beetle (Trogoderma spp.);

From Orthoptera (Orthoptera), for example,

Lian (Blatta spp.), little Lian (Blattella spp.), mole cricket (Gryllotalpa spp.), leucophaea maderae (Leucophaea maderae), migratory locusts (Locusta spp.), big Lian (Periplaneta ssp.), and grasshopper (Schistocerca spp.);

From Isoptera (Isoptera), for example,

Reticulitemes spp (Reticulitemes spp);

From Corrodentia (Psocoptera), for example,

Booklice (Liposcelis spp.);

From Anoplura (Anoplura), for example,

Blood lice (Haematopinus spp.), Linognathus spp (Linognathus spp.), lice (Pediculusspp.), pemphigus (Pemphigus spp.) and Phylloxera (Phylloxera spp.);

From Mallophaga (Mallophaga), for example

Damalinea spp. and nibble a mao lice (Trichodectes spp.);

From Thysanoptera (Thysanoptera), for example,

Flower thrips (Franklinella spp.), Hercinothrips spp (Hercinothrips spp.), Taeniothrips spp (Taeniothrips spp.), thrips (Thrips palmi), onion thrips (Thrips tabaci) and Scirtothrips aurantii (Scirtothrips aurantii);

From Heteroptera (Heteroptera), for example,

Bedbug (Cimex spp.), Distantiella theobroma (Distantiella theobroma), mock-strawberry (Dysdercus spp.), Euchistus spp., Eurygasterspp (Eurygaster spp.), Leptocorisa spp (Leptocorisa spp.), green stinkbug (Nezara spp.), lace bug (Piesma spp.), phodnius prolixus (Rhodnius spp.), Sahlbergella singularis (Sahlbergella singularis), black stinkbug (Scotinophara spp.), triatome bug (Triatoma spp.), the Miridae species, for example beanpod lygus bug and America tarnished plant bug, Lygaeidae species, for example America rough rice bar chinch bug and Pentatomiddae species;

From Homoptera (Homoptera), for example,

Wolly whitefly (Aleurothrixus floccosus), Aleyrodes brassicae, Aspidiotus (Aonidiella spp.), Aphidiadae (Aphididae), aphid (Aphis spp.), Aspidiotus (Aspidiotus spp.), Bemisia tabaci (Bemisia tabaci), lecanium (Ceroplaster spp.), Chrysomphalus aonidium (Chrysomphalus aonidium), Chrysomphalus dictyospermi (Chrysomphalusdictyospermi), Coccushesperidum (Coccus hesperidum), Empoasca spp (Empoasca spp.), Apple woolly aphid (Eriosoma larigerum), Erythroneura spp (Erythroneura spp.), Gascardiaspp., plant hopper (Laodelphax spp.), Lacanium comi, Lepidosaphesspp (Lepidosaphesspp.), Macrosiphus spp (Macrosiphus spp.), knurl aphid (Myzus spp.), Nehoteftix spp., plant hopper (Nilaparvata spp.), Paratoria spp., Pemphigus spp (Pemphigus spp.), mealybug (Planococcus spp.), white a red-spotted lizard (Pseudaulacaspis spp.), mealybug (Pseudococcusspp.), wood louse (Psylla ssp.), Pulvinaria aethiopica, Aspidiotus (Quadraspidiotusspp.), Rhopalosiphum spp (Rhopalosiphum spp.), helmet a red-spotted lizard (Saissetia spp.), leafhopper (Scaphoideus spp.), Schizaphis spp (Schizaphis spp.), Sitobion spp., greenhouse whitefly (Trialeurodes vaporariorum), Trioza erytreae and Unaspis citri (Unaspis citri);

From Hymenoptera (Hymenoptera), for example,

Acromyrmex, leaf cutting ant (Atta spp.), Cephus spp (Cephus spp.), sawfly (Diprion spp.), Diprionidae (Diprionidae), Gilpinia polytoma (Gilpiniapolytoma), real honeybee (Hoplocampa spp.), black ant (Lasius spp.), Monomoriumpharaonis, Neodiprion spp (Neodiprion spp), water ant (Solenopsis spp.) and wasp (Vespa ssp.);

From Diptera (Diptera), for example,

Yellow-fever mosquito (Aedes spp.), Antherigona soccata, Bibio hortulanus, calliphora erythrocephala (Calliphora erythrocephala), Ceratitis spp (Ceratitis spp.), gold fly (Chrysomyiaspp.), culex (Culex spp.), yellow fly (Cuterebra spp.), big trypetid (Dacus spp.), fruit bat (Drosophila melanogaster), latrine fly (Fannia spp.), stomach fly (Gastrophilusspp.), tsetse fly (Glossina spp.), torsalo (Hypoderma spp.), Hyppobosca spp., Liriomyza (Liriomysa spp.), lucilia (Lucilia spp.), the fly (Melanagromyza spp.) of diving, house fly (Musca ssp.), botfly (Oestrus spp.), Orseolia spp., Oscinella frit (Oscinella frit), Pegomyia hyoscyami (Pegomyia hyoscyami), wheat fly (Phorbia spp.), Apple trypetid (Rhagoletis pomonella), pool fly (Sciara spp.), chela fly (Stomoxysspp.), horsefly (Tabanus spp.), Tannia spp. and Tipula spp (Tipula spp.)

From Siphonaptera (Siphonaptera), for example,

C. leaf flea (Ceratophyllus spp.) and Xenopsyllacheopis (Xenopsylla cheopis), and

From Thysanura (Thysanura), for example,

Silverfiss (Lepisma saccharina).

Have been found that working as insect pest is chrysomelid species, particularly working as insect is Diabroticavirgifera virgifera (western corn rootworm, WCR), Diabrotica barberi (northern corn rootworm, NCR), Diabrotica virgifera zea (zea mexicana rootworm, MCR), Diabroticabalteata (Brazilian corn rootworm (BZR) or the Brazilian corn rootworm complex body of forming by Diabrotica viridula and Diabroticaspeciosa (BCR)), or Diabroticaundecimpunctata howardi (southern corn rootworm, SCR) time, the present invention is effective especially.

Embodiment

The contriver has identified the method that is used to control the coleopteran pest invasion and attack in this article, and this is undertaken by double stranded ribonucleic acid molecule is provided in pest food.Surprisingly, the contriver discloses, when being absorbed by insect, double stranded ribonucleic acid molecule can play a role, and suppresses the biological function of insect, causes in the following characteristics one or more: the insect feed reduces, the insect survival rate reduces, insect death, and differentiation and the growth of insect are suppressed, the reduction or the disappearance of the sexual propagation ability of insect, muscle forms, the formation of neotonin, the adjusting of neotonin, ion are regulated and transhipment, the maintenance of cell membrane potential, amino acid bio is synthetic, amino acid degradation, and sperm forms, pheromone is synthetic, the pheromone sensing, and feeler forms, wing forms, and leg forms, grow and differentiation avette one-tenth, the larva maturation, digestive ferment forms, and hemolymph is synthetic, the maintenance of hemolymph, neurotransmission, cell fission, energy metabolism is breathed, apoptosis, and any component of eukaryotic cell cytoskeletal structure, for example, Actin muscle and tubulin.Any or any combination can cause the effective inhibition to the insect invasion and attack in these characteristics, under the situation of plant insect, causes the inhibition to the plant invasion and attack.For example, when being used as when offering the foodstuffs compositions of one or more double stranded ribonucleic acid molecules plant, that contain the amount that is enough to suppress insect with local mode, be used as seed treatment, as the soil application around the plant, when the recombinant DNA molecules that perhaps exists in vegetable cell comes by plant production, can unexpectedly reduce significantly the plant insect invasion and attack.When being applied to list kind insect, the embodiment shown in this paper is used to set forth the present invention.But, those skilled in the art will recognize that, method among the embodiment, prescription and idea are not made the usefulness of restriction, it can be used for consuming all lepidoptera pest species of following food source, to suppress relevant or some key characters of playing a role of portion within it with insect, described food source is to be configured to the insect inhibitor that contains q.s, and it is made of at least a or multiple double stranded rna molecule.

Embodiment 1

Be used for controlling the evaluation of the target nucleotide that the preparation of the dsRNA of corn rootworm uses

From whole larva, pupa and make up corn rootworm cDNA library from the midgut segment of cutting (LIB 149, LIB 150, LD33027, LIB3373), obtain nucleotide sequence information and (see that people such as Andersen are filed in the U.S. Patent Application Serial Number 10/205 on July 24th, 2002,189, its integral body is incorporated into this paper by reference especially).In addition, from being in different developmental phases and the whole larva of different time each etap, come the construction cDNA library, with the quantity of maximization from the different est sequences of chrysomelid species.Library LIB5444 and LIB5462 are constructed in the mRNA set that is obtained by the western corn rootworm larva from first age (1 gram) and the 3rd age (2.9 gram) respectively.By being inserted in the liquid nitrogen, that the insect that obtains is freezing rapidly.Remaining in-20 ℃ mortar and the pestle and grinding insect, temperature keeps by in refrigeration on the dry ice and/or add liquid nitrogen ground to fine powder up to tissue and realize in mortar.Use TRIzol

Reagent (Invitrogen) according to manufacturers instruction, extracts RNA.(Dynal Inc. NY), according to manufacturers instruction, isolates Poly A+RNA from total RNA preparation to use Dynabeads Oligo dT.Use SuperScript ^TMPUC pUC (Invitrogen) comes the construction cDNA library from Poly A+RNA.Using chromatogram that cDNA is carried out size fractionation separates.The fourth stage divides and level V branch fragment is collected, and be connected into pSPORT1 carrier (Life Technologies Ind., Gaithersburg MD) between Sal1 and the Not1 restriction enzyme enzyme recognition site, transforms into intestinal bacteria DH10B electroreception attitude cell by electroporation.The first instar larvae library has produced about 420,000 colony-forming units.The third-instar larvae library has produced about 2.78 * 10 ⁶Individual colony-forming unit.Wash bacterium colony from flat board,, pour the Tris-EDTA damping fluid again into by the short term oscillation uniform mixing from LIB 149, LIB150.In the material that washes half is loaded into 10% glycerine, divides and puts into freezing tubule, is stored in-70 ℃.Second half is used to produce plasmid DNA with Quiagen trace purification column or its equivalent.Divide the threading micro-centrifuge tube with purified plasmid DNA, be stored in-20 ℃.

In the high viscosity substratum, increase respectively from the bacterium colony of Diabrotica virgifera cDNA library LIB5444 and LIB5462.Under 37 ℃, (contain 0.3%SeaPrep agarose at the 500ml LB substratum that stirs flat board respectively

With the 50mg/l Pyocianil) in, mixing from about 200,000 colony-forming units of LIB5444 with from 600,000 colony-forming units of LIB5462, cooling 1 hour rapidly in water/ice bath then makes bacterial colony evenly suspend.Make through the library of inoculation at 30 ℃ then and grew 42 hours.After the incubation, pair cell carries out 5 minutes mixing on the stirring flat board.Then in the centrifugal bottle with media transfer to two 250ml.10,000xg precipitum cell 10 minutes.From bottle, shift out substratum, cell is suspended in again in the LB substratum of 20ml altogether, wherein contain the Pyocianil of 50mg/l.Add methyl-sulphoxide to 10%, so that cell is remained freezing state.Increased in two kinds of libraries, to every milliliter 10 ⁸The final titre of individual colony-forming unit.The sample of combination Diabroticavirgifera cDNA library LIB5444 and LIB5462, in through aseptic distillation and deionized water, it is adjusted to the DNA concentration of about 1.25 micrograms of every microlitre, divides and put into 25 freezing tubules, each freezing tubule contains the DNA of about 8.75 micrograms.Applicant/contriver on June 10th, 2004 with these sample preservations to being positioned at 10801 University Boulevard, Manassas, the American type culture collection (ATCC) of Virginia USA ZIP 20110-2209, it is called as LIB5444/62.ATCC provides preservation to prove to the applicant, and it specifies the ATCC preserving number is PTA-6072.

Substantially produce the method in corn rootworm cDNA library according to mentioned above being used to, preparation corn rootworm high molecular cDNA library, i.e. LIB5496 and LIB5498.Make up library LIB5496 and LIB5498 from the mRNA storehouse that (1 gram) western corn rootworm larva obtains by first age (1 gram) and the second and the 3rd age respectively.In brief, rapid freezing insect by milling, becomes fine powder with the refrigerated insect in mortar and pestle in liquid nitrogen.Use TRIzol

Reagent (Invitrogen) according to manufacturers instruction, extracts RNA.(Dynal Inc. NY), according to manufacturers instruction, isolates Poly A+RNA from total RNA preparation to use Dynabeads Oligo dT.Use SuperScript ^TMPlasmid System (Invitrogen) makes up high molecular cDNA library from the PolyA+RNA of 20 micrograms.On 1% sepharose in TAE, cDNA is carried out size fractionation separate.Collect the cDNA in 1Kb to the 10Kb scope, connect between the Sal1 and Not1 restriction site of pSPORT1 carrier into, transform into intestinal bacteria DH10B electroreception attitude cell by electroporation.LIB5496 has produced about 3.5 * 10 ⁶Individual colony-forming unit.LIB5498 has produced about 1.0 * 10 ⁶Individual colony-forming unit.Single amplification is from the bacterium colony of corn rootworm high molecular cDNA library LIB5496 and LIB5498 in the high viscosity substratum.Under 37 ℃, (contain 0.3%SeaPrepagarose at the 500ml LB substratum that stirs flat board respectively

With the 50mg/l Pyocianil) in, mix about 600,000 colony-forming units from LIB5496 and LIB5498, in water/ice bath, cooled off 1 hour rapidly then, make bacterial colony evenly suspend.At 30 ℃ grown 42 hours in the library of inoculation then.After the incubation, pair cell carries out 5 minutes mixing on the stirring flat board.Then in the centrifugal bottle with media transfer to two 250ml.10,000xg handled 10 minutes, the precipitum cell.From bottle, shift out substratum, cell is suspended in again in the LB substratum of 20ml altogether, wherein contain the Pyocianil of 50mg/l.Add methyl-sulphoxide to 10%, so that cell is remained freezing state.Increased in two kinds of libraries, to every milliliter 10 ⁸The final titre of individual colony-forming unit.Obtain the cDNA sequence information of insertion from corn rootworm species specificity plasmid library.

People's such as Andersen corn rootworm library and produce about 18,415 independent est sequences from the additional sequences in LIB5444 and LIB5462 library is initial, it is by about 1.0 * 10 ⁷Individual nucleotide residue constitutes.The mean length of est sequence is about 586 nucleotide residues.These est sequences are used to the information biology algorithm, obtained the assembling of contig (contig) sequence, this is called as the UNIGENE sequence in this article, can not be called as singlet (singleton) in this article by each est sequence of overlapping identity and the assembling of other est sequence.Then LIB5444 and LIB5462 library are carried out the more order-checking of the degree of depth, obtain extra individual est sequence.Will be from the library, be that the est sequence that LIB149, LIB150, LIB3027, LIB3373, LIB5444, LIB5462, LIB5496 and LIB5503 obtain is selected to the further research that consumer organism is hereinafter described measured, and corresponding sequence is shown in the sequence table.

If possible, will separate the est sequence that obtains from CRW cDNA library and be assembled into the UNIGENE group, the Unigene sequence of these equipments is included in the sequence table.UNIGENE be the gene orientation bunch, it forms from single est sequence overlapping in sequence identity zone, to form bigger sequence.People such as Pontius (2003).Every nucleotide sequence shown in the sequence table is analyzed, identified the existence of reading frame.The amino acid sequence information that to infer from reading frame and the known amino acid sequence information that can obtain from public database relatively, to infer the degree of aminoacid sequence and these known amino acid sequence identity or similarity.If exist, use the biological function relevant with known amino acid sequence in the public database, add following note for the aminoacid sequence of inferring from cDNA library nucleotide sequence information.Note provides protein (may express from the specific gene that the provides specific cDNA sequence) function information of hint property, but this and indecisive result.Annotation information based on hint property, some cDNA sequence is accredited as: coding may relate to the albumen of some biological functions in the corn rootworm cell, described function is very important to life, be necessary perhaps, may relate to perhaps that cell is complete, cell maintenance, fecundity etc. the health of guaranteeing cell and survival.

The sequence of selecting to be used for further research is used for the dsrna construct molecule, mixes CRW food.It is right to design hot amplimer based on cDNA and EST homing sequence, obtains to be used to the sequence of ingesting and measuring.Primer to or be built as a pair of nucleotide sequence, each right member of primer shows and the complete complementarity that justice or antisense sequences are arranged.Make up some primers to sequence, make each member of this centering show the sequence that contains T7 phage rna polymerase promotor at 5 ' end.Preferably, use crw genomic dna, increase for the first time to carrying out high-fidelity, produce first amplicon with first primer that lacks the T7 promotor as template.Preferably, cDNA or mRNA sequence are used as template, are used for the dsRNA molecule that synthetic the present invention uses, because eukaryotic gene group sequence is considered to contain non-existent sequence in the mature rna molecule in this area.To be used as template the hot amplified reaction for the second time from the first amplicon sample of high-fidelity amplified reaction generation for the first time then, with the second pair of primer that contains the T7 promoter sequence to producing second amplicon, wherein, contain in 5 ' end of every chain of second amplicon or wherein be embedded with the T7 promotor.Obtain the complete nucleotide sequence of second amplicon with both direction, itself and nucleotide sequence at the cDNA report are compared, and, if exist, point out the difference between the two sequences.Usually, use genomic dna as the sequence of template preparation be used to obtain that conspicuous level is prevented, as the further use of dsRNA molecule and inconsistent, because the variation of non-existent genome sequence in mRNA or cDNA sequence is arranged.

Typically, the in-vitro transcription reaction contains the about 1 linearizing dna profiling to about 2 micrograms, from the T7 polymeric enzyme reaction damping fluid of 10X concentrated solution, the T7 RNA polymerase of ribonucleotide ATP, CTP, GTP and UTP (wherein concentration is that each is 50 to 100mM) and 1 unit.According to manufacturers instruction, at about 37 ℃ RNA polymerase reacted incubation for some time (several minutes to a few hours), this depends on the optimum temps of used RNA polymerase.Usually, reaction carries out about 2 to about 6 hours, is used to transcribe the template sequence that reaches about 400 Nucleotide most, and reaches 20 hours most, is used to transcribe the template sequence of length greater than about 400 Nucleotide.Reaction is heated to 65 ℃, continues 15 minutes, to stop rna transcription.Precipitated rna transcription product in ethanol, the washing, at air drying, it is suspended in the water that does not contain the RNA enzyme again, to concentration be every milliliter of about 1 microgram.Utilize most of transcript of reverse T7 promotor strategy (being summarized as mentioned) to produce double-stranded RNA in the responsive transcription in vivo, but, by purified RNA is heated to 65 ℃, slowly cool to room temperature then to guarantee that justice and the segmental correct annealing of sense-rna are arranged, obtained the double-stranded RNA of high yield.At 37 ℃ the double-stranded RNA product is carried out one hour incubation with DNA enzyme I and RNA enzyme then, to remove any DNA or the single stranded RNA that exists in the mixture.According to manufacturers instruction (AMBION MEGAscript

RNAi KIT), purifying double-stranded RNA product on pillar is suspended in 10mM Tris-HCl damping fluid (pH 7.5) again with it or does not contain in the water of RNA enzyme, to the concentration of every microlitre 0.1 to 1.0 microgram.

Following nucleotide sequence is initial as the obtained (people such as Andersen of the cDNA sequence that identifies in corn rootworm midgut cDNA library, the same), use it for the dsrna construct molecule, be used for upchecking in the pest food inhibition effect of double stranded rna molecule of ingesting to the insect biological function.

The A.Chd3 homologous sequence

Identified the CHD gene in multiple eukaryote, its corresponding albumen is considered to play a role as the chromatin reconstruct factor.Term CHD is believed for every kind and can be given different chromatin related activity from three kinds of sequence homology structural domain: chromo that find in the CHD albumen (chromatin group structure modifier) structural domain, SNF2-relational spiral enzyme/ATP enzymatic structure territory and DNA binding domains.Based on the whether existence of another kind of sequence homology structural domain (PHD Zinc finger domain, typically, relevant with the karyomit(e) related activity), CHD albumen is divided into two classes.The CHD3 associated protein has the PHD Zinc finger domain, but the CHD1 associated protein is then really not so.Experimental observation has hinted the effect of CHD3 albumen in inhibition is transcribed, and it has been shown as in some species and has contained the composition of histone deacetylase enzyme as the complex body of subunit.The deacetylation of histone is with to transcribe deactivation relevant, so CHD3 albumen is implied to be: utilize the character as the composition of Histidine deacetylase complex body, people such as (, 1999) Ogas can play a role as transcription inhibitor.Therefore, CHD3 albumen synthetic prevented can be used as useful target, be used for the inhibition to coleopteran pest of double chain RNA mediate.

B. 'beta '-tubulin homologous sequence

Tubulin is the important structure component of various kinds of cell structure in all eukaryotic cells, is mainly used in the formation microtubule.The inhibition that microtubule forms in the pair cell can cause serious consequence, comprises, to the interference that mitotic spindle forms, pair cell splitted blocking-up etc.Therefore, the inhibition that tubulin is formed can be the useful target of the inhibition of double chain RNA mediate.

C.40kDa V-AT enzyme homologous sequence

Energy metabolism in the eukaryote system in the subcellular organelle is unusual important function.The vacuole atp synthase participates in the ATP that keeps enough levels in the vacuole.Therefore, the vacuole atp synthase can be the useful target of the inhibition of double chain RNA mediate.

D.EF1 α homologous sequence

Transcription elongation and transcription termination factor are very important for metabolism, and they can be the favourable targets that is used for the inhibition of double chain RNA mediate.

E.26S proteoplast subunit p28 homologous sequence

The 26S proteoplast is big, dependency ATP, multimeric protein enzyme, and it is conservative at all eukaryote camber.It is removed in the proteic process of multiple short life in selectivity has general function, and described albumen was covalently bound with ubiquitin before this, was degraded by 26S proteolytic enzyme complex body then.The ubiquitin approach has important effect in the control of cell cycle, described control realizes by the proteic specificity of multiple adjusting is degraded, described albumen comprises the mitotic cell cyclin and to the kinase whose inhibitor of cyclin dependent, for example, the p27 of mammalian cell.Therefore, 26S proteoplast synthetic being prevented and it is formed that subunit synthetic suppresses can be preferred target people such as (, 1997) Smith of inhibition of double chain RNA mediate.

F. neotonin epoxide hydrolase homologous sequence

Corpus allatum hormone is controlled and is regulated the biological procedures of multiple necessity in the insect life cycle, and described process is including but not limited to metamorphosis, breeding and diapause.In suitable, neotonin (JH) concentration need reach peak value in the hemolymph of insect pest larva (particularly lepidopteran and coleoptera larvae) form, and it must be degraded then, to stop the hormone response effect.The enzyme that relates in the process of reduction neotonin concentration plays a role by two main paties of metabolic degradation.Article one, approach relates to juvenile hormone esterase (JHE), and its energy hydrolysis methyl esters provides corresponding acid.Second kind of approach utilizes neotonin epoxide hydrolase (JHEH), to obtain the hydrolysis to epoxide, causes glycol to form.The contribution of JHE in the JH degraded understood well, finds that it is constant between lepidopteran and Coleoptera species.Inhibition to the JH esterase is relevant with serious profile variation, includes but not limited to, larva migration (larvalwandering), pupates to postpone and the growth of lopsided intermediate state.On the contrary, people are not enough to the effect understanding of JHEH in the JH metabolism, and, it has demonstrated between species and can change, JHEH may be evidence (the Brandon J.Fetterolf of the metabolic main path of JH but hint has been pointed out in recent research, Doctoral Dissertation, North Carolina StateUniversity (Feb.10,2002) Synthesis and Analysis of Mechanism BasedInhibitors of Juvenile Hormone Epoxide Hydrolase from InsectTrichoplusia ni).At any time, can be to be used for effective target that the insect of double chain RNA mediate suppresses to the interference of any JH degradation pathway with the gene technology of preventing.

G. rely on the chloride channel albumen homology sequence of swelling (swelling dependent)

Relying on swollen chloride channel albumen is assumed to be: play the part of pivotal player in the osmoregulation of eucaryon zooblast system.Therefore, the nucleotide sequence that shows the ability that can express following aminoacid sequence can be to be used for carrying out the useful target that RNA suppresses insect, and described aminoacid sequence shows and the proteic homology of dependence swollen chloride channel that identified in the past.

H. G-6-P 1-apodehydrogenase homologous sequence

G-6-P 1-apodehydrogenase (G6PD) catalysis G-6-P is oxidized to 6-phosphogluconate, follows the oxidised form with Triphosphopyridine nucleotide, reduced (NADP+) to be reduced to NADPH simultaneously.Known to the essential cofactor in a variety of eukaryote building-up reactionss in NADPH this area, it is its reduction form that known its can keep gsh.The gsh that is reduced plays a role as the scavenging agent of dangerous oxidative metabolism product in eukaryote, and, under the assistance of Selenoperoxidase, deleterious hydrogen peroxide is converted into water (people such as Beutler, 1991).Therefore, G6PD can be the preferred target of the inhibition in coleopteran pest of double chain RNA mediate.

I.Act42A albumen homology sequence

Actin muscle is an eukaryotic protein ubiquitous, high conservative, and it is cell movement and location needed people such as (, 2001) Lovato.Identified a large amount of CRW cDNA sequences, they are predicted to be may encode Actin muscle or show the albumen of the aminoacid sequence structure relevant with Actin muscle.Therefore, the gene of coding Actin muscle homologue can be the useful target of the inhibition of double chain RNA mediate in the insect cell.

J.ADP-ribosylation factor 1 homologous sequence

ADP ribosylation factor 1 has been shown as in cell function very important, because they embody complete effect in the process of dna damage reparation, carcinogenic, necrocytosis and genome stability.Therefore, the inhibition with double chain RNA mediate optionally destroys transcribing of ADP-ribosylation factor in the coleopteran pest species, will be useful.

K. transcription factor IIB albumen homology sequence

As mentioned before, the transcription elongation and the translation termination factor are extremely important for metabolism, can be the favourable targets of the inhibition of double chain RNA mediate, are used to control or eliminate the invasion and attack of coleopteran pest.

L. chitinase protein

Chitin is β (1 → 4) homopolymer of N-acetylglucosamine, and it is found in the insect exoskeleton.Chitin forms from the UDP-N-acetylglucosamine in the catalytic reaction of chitin synthase.Chitin is structural homopolysaccharide, in the structure to this hyperbranched and crosslinked structure, relates to a lot of enzymatic steps.Chitin gives insect shape, hardness and support, and the internal is provided, for example the support that adhered to of muscle.Chitin also is degraded to a certain degree, with the step that relates in the mediation insect molting process.Therefore, it is believed that, double chain RNA mediate, to proteic inhibition in these approach, will can be used as the means of control coleopteran pest invasion and attack.

M. ubiquitin conjugated enzyme homologous sequence

The ubiquitin approach has important effect in the control of cell cycle, this realizes that by the proteic specificity of a large amount of adjustings is degraded described albumen comprises the kinase whose inhibitor of mitotic cell cyclin and cyclin dependent, for example, the p27 of mammalian cell.Therefore, the gene of coding ubiquitin and related component can be the preferred target of the inhibition of double chain RNA mediate people such as (, 1997) Smith.The proteolysis approach that relies on ubiquitin is one of main path of the interior albumen selective destruction of pair cell in the eukaryote.The puting together of ubiquitin and substrate protein being subjected to obviously to be multifarious enzyme array regulates.The proteolysis target can be that step between the peptide is regulated by many subunits 26S proteasome degradation at the ubiquitinization of substrate and its also surely.The complicacy of ubiquitin system has hinted its central role that upgrades for albumen in the eukaryotic cell adjusting, and involves other albumen in the approach, comprises ubiquitin activating enzyme, ubiquitin binding enzyme, ubiquitin conjugated enzyme and 26S proteoplast subunit component.Therefore, it is believed that the inhibition to this pathway protein of double chain RNA mediate will can be used as the means of control coleopteran pest invasion and attack and use.

N. glyceraldehyde-3-phosphate dehydrogenase homologous sequence

Glycolytic pathway is important approach very in most of biologies, and it relates to from degradation of glucose and produces metabolisable energy.In the subordinate phase of glycolytic pathway, an important enzyme is glyceraldehyde-3-phosphate dehydrogenase (G3PDH), and under the situation that has NAD+ and inorganic phosphate, it can be oxidized to glycerol 3-phosphate-phosphoric acid by the catalysis glyceraldehyde 3-phosphate, forms NADH thereupon.The important component of this reaction is that energy stores by the formation of NADH.The coding and the gene of glycolytic pathway involved enzyme, and especially, coding participate in being used to form the gene of the enzyme in the step of energy storage, can become the useful especially target of the inhibition of carrying out double chain RNA mediate in the coleopteran pest thing.

O. ubiquitin B homologous sequence

As mentioned above, the ubiquitin protein degradation pathway has important effect in the control of cell cycle, this realizes by the proteic specificity of a large amount of adjustings is degraded, described albumen comprises the mitotic cell cyclin, or the kinase whose inhibitor of cyclin dependent, for example, the p27 of mammalian cell.Therefore, the gene of coding ubiquitin and related component can be the preferred target that is used for the inhibition of double chain RNA mediate people such as (, 1997) Smith.

P. juvenile hormone esterase homologue

As indicated above, corpus allatum hormone is controlled and is regulated the biological procedures of multiple necessity in the insect life cycle, and it includes but not limited to, metamorphosis, breeding and diapause.Effective target of the insect control that the interference synthetic to JH or degradation pathway of use gene inhibition technology may be a double chain RNA mediate.

Q. α tubulin homologous sequence

Eukaryotic cells utilizes the cytoskeletal structure element usually, and they are important, not only as machinery mount, also keeps cell shape.Semiflexible microfilament makes cell to move, and helps them to divide in mitotic division (division of cytoplasm), and, in vertebrates and invertebrates, be used for Muscle contraction.Relatively harder microtubule is made of α and beta tubulin, and they have important effect in following process: as sending a cover motorway of passing vacuole and organoid to play a role, and be used for making during mitotic division (nuclear fission) karyomit(e) separately.Flexible intermediate filament provides for whole cyto-architectural extra at least intensity.Also known, cytoskeleton participates in striding cytoplasmic signal transduction process.Consider these functions, we believe, any destruction of pair cell skeleton or even all may cause the pathological examination of pair cell to the minor alteration of its integrity.

R. transport correlated series

As indicated above, sorting and the multiple molecule of transhipment comprise sorting and are transported to suitable organoid in cell, and their secretion, are a kind of important physical functions.Described sorting approach can comprise depending on transports required interior body sorting mixture (ESCRT), i.e. those of composite I-III.Therefore, relevant with the transhipment of polypeptide and other molecule function also can be the preferred target of the inhibition of dsRNA mediation.

Embodiment 2

Insect feeds raises biological assay

Double-stranded RNA (dsRNA) sample is measured, and this measures the target insect of adopting selected number.Prepare dsRNA from the sequence of identifying according to embodiment 1, wherein use whole contig sequence (under the situation of SEQ ID Nos:1-6), or the sequence that the contig from assembling is increased with primer shown in the sequence table.According to following program, the dsRNA of various dose is administered on the corn rootworm artificial food as tectum.From Crop Characteristics, Inc., Farmington, Minnesota obtains the ovum of Diabrotica virgifera virgifera (WCR).In soil, in 24 ℃, 60% relative humidity, fully down dark, the WCR ovum of non-duration of diapause is carried out about 13 days cultivation.At the 13rd day, the soil that will contain the WCR ovum was placed in the #30-#60 purpose sieve, uses high pressure garden water pipe flush away soil.Carry out disinfection by three minutes surfaces to ovum of bubble in LYSOL, it is inferior to give a baby a bath on the third day after its birth with aqua sterilisa, washes once with 10% formalin solution, washes extra three times with sterilization again.The ovum of handling in this way is distributed in the sterilization coffee strainer, at 27 ℃, 60% relative humidity, down dark fully, hatching is spent the night.

In order to prepare dsRNA, with the amplicons cloned of the sequence selected in the plasmid vector that can in intestinal bacteria, duplicate, reclaim the plasmid DNA of q.s, carry out external T7 rna polymerase transcribe from the convergent T7 promotor of the embedding of any end of cloned sequence with permission.Produce double-stranded RNA, carry out biological assay; Article one, the RNA fragment comprises the sequence shown in the sequence table, and another RNA fragment is the reverse complementary sequence of this nucleotide sequence substantially, and wherein, guanosine suitably replaces thymidine.With the sample of DICER or RNA enzyme III processing double-stranded RNA (dsRNA), to produce the siRNA (siRNA) of q.s.The sample that will contain siRNA or dsRNA covers the CRW food of above describing and measures, according to larva is ingested.

The double-stranded RNA sample is directly joined in each hole of containing the insect food of above pointing out, perhaps before joining insect food, it is modified.To the modification of double-stranded RNA according to manufacturer provide at RNA enzyme III (AMBION CORPORATION, Austin, Texas) or DICER (specification sheets California) carries out for STRATAGENE, La Jolla.RNA enzyme III has produced the duplex of 21 and 22 Nucleotide to the digestion of double-stranded RNA, 3 ' the C-terminal that it contains the end of 5 ' phosphorylation and has 2-3 base overhang, be similar to double-stranded short interfering rna (siRNA) fragment of about 21-26 base pair, this is the dicer enzyme generation in people (2001a) evaluation, the eucaryon approach such as people such as Hamilton (1999) and Elbashir.This short interfering rna duplex of collecting is further purified, sample is analyzed, to measure integrity and the efficient that duplex forms with polyacrylamide gel electrophoresis.Come the purity and the quantity of working sample then under the wavelength of 250 nanometers by spectrophotometry, untapped sample is stored in-20 ℃, is used for further use.

Substantially according to the described insect food of making of people such as Pleau (2002), wherein made following change.The SERVA agar of 9.4 grams is distributed to 540 milliliters of pure water, stirs and disperse fully until agar.Water/agar is mixed and heated to boiling,, topples over into WARING stirrer then to dissolve agar fully.Stirrer is remained low speed, and join 62.7 gram BIO-SERV DIET mixtures (F9757), the freeze dried corn root of 3.75 grams, 1.25 milliliters of non-polluted edible pigment and 0.6 milliliter of formalin in the agar mixture of heat this moment.Be 9.0 by adding 10% potassium hydroxide storage liquid with the mixture pH regulator then.On the magnetic agitation hot plate, use the magnetic force splash bar of sterilization NALGENE coating, mix continuously with liquid food at a high speed, and it is remained about 48 ℃ to about 60 ℃, and it is separated into 200 microlitre equal portions in each hole of titer plate at the bottom of the FALCOM96 hole circle about 600 ml volumes.In the biological exhausting cupboard (biohood) of sterilization, make about 10 minutes of foodstuff solidification in the flat board and dry air.

Use running fire type micropipet, get the test sample of 30 (30) microlitre volumes, wherein contain the contrast agents or the double-stranded RNA of different quantities, it is covered insect foodstuff surface in each hole.After the application test sample, insect food is allowed to keep reaching one and a half hours in the biological exhausting cupboard of sterilization, so that diffusion of reagents feed thing, and allow the foodstuff surface drying.In each hole, put into a WCR newborn larvae with paintbrush.Use the MYLAR seal plate then, ventilate with insect needle.At every kind of dosage, 12-72 insect larvae to be tested, quantity depends on test design.At 27 ℃, 60% relative humidity and the dark fully incubation that down the biological assay flat board is carried out 12-14 days.Time point at 12-14 days is to the following quantity record in addition of survival larva of every kind of dosage.Use suitable microbalance,, measure the larva quality at the larva of every survival.Use JMP

4 statistical softwares (SAS Institue, 1995) come analytical data, check with Dunnet and carry out complete factorial ANOVA, with the effect (P＜0.05) of seeking processing than untreated control.Carry out Tukey-Kramer (post hoc) check afterwards, come relatively more all processing (P＜0.05).The CRW larva feeds the result who raises mensuration and has shown with respect to the significant growth-inhibiting and the death that contrast, as explanation hereinafter.

Embodiment 3

Insect feeds the result who raises biological assay

By using the biological assay of adopting embodiment 2 to describe, the sample of the double-stranded RNA sequence of identifying according to the description of embodiment 1, the artificial food that preparation is enough to raise the corn rootworm larva.Typically, make ingest above-mentioned food 12 days of corn rootworm larva, monitoring is with respect to the death and the hypoevolutism of only feeding the rootworm of raising negative and positive control food.The result of study proof is with containing and multiple different genes type homologous sequence dsRNA partly, and the larvae development that produces conspicuous level (p＜0.05) is slow and/or dead.Following table 1-5 has shown sequence and the carrier that produces remarkable hypoevolutism and/or death, and the corresponding sequence numbering that is expressed as the sequence of dsRNA.

Table 1: the insect that carries out with southern corn rootworm or western corn rootworm feeds to raise and has confirmed remarkable hypoevolutism and/or dead dsRNA construct in the biological assay

Carrier	Be expressed as the sequence of dsRNA	SEQ ID NO
			RNAi-pIC17553：001	Apple	697
RNAi-pIC17504：049	EST total length V-ATP enzyme	695
			RNAi-pIC17554：001	Rpl 9	698
RNAi-pIC17555：001	Rpl 19	699
			RNAi-pIC17504：050	6.0 part of V-ATP enzyme	711
RNAi-pIC19514：001	WCR mRNA capping enzyme LIB5496-028-A1-M1-A7	696
			RNAi-pIC17552：003	The black dull structural constituent of LIB5462-042-A1-M1-H10 Dv.6_CG9355 epidermis	700
RNAi-pIC17546：003	LIB5462-091-A1-M1-G3 Dv.1_CG6217KNICKKOPF UNK	701
			RNAi-pIC17546：001	Dv.1_CG6217	1
RNAi-pIC17549：001	Dv.4_CG1435	4
			RNAi-pIC17550：001	LIB5444-065-A1-M1-D5 Dv.5_cg1915_1	5
RNAi-pIC17551：001	LIB5462-012-A1-M2-B2 Dv.5_cg1915_2	703
			RNAi-pIC17552：001	Dv.6_CG9355	6
RNAi-pMON78412：002	Dv.7_CG3416; The Mov34:CG3416 that infers is directly to homologue	10
			RNAi-pMON96172：002	Dv.8_CG1088; The vacuole H+ATP enzyme E subunit of inferring: CG1088 is directly to homologue	14
RNAi-pMON96168：002	Dv.9_CG2331; The atpase activity of inferring: CG2331 is directly to homologue	18
			RNAi-pMON78424：002	Dv.10_CG6141; The ribosomal protein L 9:CG6141 that infers is directly to homologue	22
RNAi-pMON78425：001	Dv.11_CG2746	26

RNAi-pMON78444：002	Dv.12_CG1341; The proteasome of inferring is regulated particle, and rpt1:CG1341 is directly to homologue	30
			RNAi-pMON78416：002	Dv.13_CG11276; The ribosome protein S 4-9 of inferring: CG11276 is directly to homologue	34
RNAi-pMON78434：001	Dv.14_CG179272; The myoglobulin heavy chain of inferring: CG17927 is directly to homologue	38
			RNAi-pMON78439：001	Dv.16_CG5394; Glutamy-the Alanyl-tRNA synthetase of inferring: CG5394 is directly to homologue	46
RNAi-pMON78438：001	Dv.17_CG10149; The proteasome p44.5 subunit of inferring, rpn6:CG10149 is directly to homologue	50
			RNAi-pMON78435：002	Dv.18_CG1404; The RAN minor comonomer GTP enzyme of inferring: CG1404 is directly to homologue	54
RNAi-pMON78449：002	Dv.19_CG18174; The proteasome of inferring is regulated particle, covers inferior mixture, and rpn11:CG18174 is directly to homologue	58
			RNAi-pMON78419：001	Dv.20_CG3180_1; The rna plymerase ii that is oriented to DNA of inferring: CG3180 is directly to homologue	62
RNAi-pMON78440：002	Dv20_CG3180_2; The rna plymerase ii that is oriented to DNA of inferring: CG3180 is directly to homologue	706
			RNAi-pMON78420：001	Dv.21_CG3320; The Rab1:CG3320 that infers is directly to homologue	70
RNAi-pMON78410：002	Dv.22_CG3395; The ribosomal protein S9:CG3395 that infers is directly to homologue	74
			RNAi-pMON78422：001	Dv.23_CG7269; The helicase of inferring: CG7269 is directly to homologue	78
RNAi-pMON78423：001	Dv.25_CG9012; The clathrin heavy chain of inferring: CG9012 is directly to homologue	86
			RNAi-pMON78414：006	5 ' part of the about EST of DV.26sec1	710
RNAi-pMON78414：001	Dv.26_CG9261; The sodium of inferring/potassium exchange ATP enzyme: CG9261 is directly to homologue	90

RNAi-pMON78413：001	Dv.27_CG12052; The rna plymerase ii transcription factor of inferring: CG12052 is directly to homologue.	94
			RNAi-pMON78427：001	Dv.35_CG3762; The Vha68-2:CG3762 that infers is directly to homologue.	126
RNAi-pMON97122：001	C1_Dv.35; The Vha68-2:CG3762 that infers is directly to homologue; Concatermer	713
			RNAi-pMON97127：001	C2_Dv.35; The Vha68-2:CG3762 that infers is directly to homologue; Concatermer	714
RNAi-pMON97125：001	C3_Dv.35; The Vha68-2:CG3762 that infers is directly to homologue; Concatermer	715
			RNAi-pMON78441：001	Dv.39_CG9078; Sphingolipid δ-4 desaturase of inferring; Stearyl-CoA 9-desaturase: CG9078 is directly to homologue	142
RNAi-pMON97114：001	Dv.41_CG2637; Female sterile Ketel; Participating in albumen-nucleus enters: CG2637 is directly to homologue	150
			RNAi-pMON97140：001	Dv.44_CG1244; The nucleic acid of inferring in conjunction with activity: CG1244 directly to homologue	162
RNAi-pMON78429：001	Dv.46_CG10689; The rna helicase enzyme of inferring: CG10689 is directly to homologue	170
			RNAi-pMON78432：001	Dv.48_CG33196; The transmembrane receptor protein Tyrosylprotein kinase of inferring: CG33196 is directly to homologue	178
RNAi-pMON78428：001	Dv.49_CG8055_1; The combination of inferring, carrier activity: CG8055_1 is directly to homologue	182
			RNAi-pMON78428：003	Dv.49_CG8055_1; The combination of inferring, carrier activity: CG8055_1 directly is selected from the free district of DV.49 to homologue	704
RNAi-pMON78426：001	Dv.50_CG10110_1; Cutting of inferring and polyadenylation atopen: CG10110_1 are directly to homologue	186
			RNAi-pMON96185：001	Dv.55_CG5931; The splicing factor activity of inferring, RNA helicase activity: CG5931 is directly to homologue	202

RNAi-pMON78442：001	Dv.57_CG2968; The hydrogen output ATP enzyme of inferring: CG2968 is directly to homologue	206
			RNAi-pMON78431：001	Dv.58_CG1751; The signal peptidase of inferring: CG1751 is directly to homologue	210
RNAi-pMON96177：001	Dv.61_CG3725_1; The calcium atpase of inferring: CG3725 is directly to homologue	222
			RNAi-pMON96183：001	Dv.62_CG3612; The lead of inferring: CG3612 is directly to homologue	230
RNAi-pMON96180：002	Dv.65_CG7033; The chaperone activity of inferring: CG7033 is directly to homologue	242
			RNAi-pMON96176：001	Dv.66_CG32019; The bent:CG32019 that infers is directly to homologue	246
RNAi-pMON96170：002	Dv.67_CG16916; The endopeptidase activity of inferring: CG16916 is directly to homologue	250
			RNAi-pMON96166：001	Dv.70_CG5771; The Rab-protein 11 of inferring: CG5771 is directly to homologue	258
RNAi-pMON96179：001	Dv.72_CG6831; The rhea:CG6831 that infers is directly to homologue	266
			RNAi-pMON96186：001	Dv.73_CG10119; The lamin C:CG10119 that infers is directly to homologue	270
RNAi-pMON96160：002	Dv.74_CG6375; The pitchoune:CG6375 that infers is directly to homologue	274
			RNAi-pMON97137：001	Dv.77_CG4214; The syntaxin 5 of inferring; The transhipment of participation intracellular protein: CG4214 is directly to homologue	286
RNAi-pMON96167：001	Dv.82_CG8264; The Bx42:CG8264 that infers is directly to homologue	302
			RNAi-pMON96171：001	Dv.83_CG11397; The gluon:CG11397 that infers is directly to homologue	306
RNAi-pMON96187：003	Dv.85_CG4494; The protein binding activity of inferring: CG4494 is directly to homologue	314

RNAi-pMON96174：001	Dv.86_CG5055; The bazooka:CG5055 that infers is directly to homologue	318
			RNAi-pMON97126：001	Dv.88_CG8756; Unknown Function; Contain chitin binding domains: CG8756 directly to homologue	326
RNAi-pMON97130：001	Dv.93_CG8515; The epidermal structure component of inferring; Contain chitin binding domains: CG8515 directly to homologue	342
			RNAi-pMON97109：001	Dv.99_CG2446; Unknown; Cause death in fruit bat and have low homology with the people: CG2446 is directly to homologue	366
RNAi-pMON97111：001	Dv.105_CG1250_1; The GTP zymoexciter, participate in the intracellular protein transhipment: CG1250 is directly to homologue	390
			RNAi-pMON97112：001	Dv.105_CG1250_2; The GTP zymoexciter, participate in the intracellular protein transhipment: CG1250 is directly to homologue	394
RNAi-pMON97107：001	Dv.107_CG14813; COPI vacuole tunicle; Participate in the intracellular protein transhipment of golgi body to endoplasmic reticulum: CG14813 is directly to homologue	398
			RNAi-pMON97115：001	Dv.108_CG17248; N-synaptobrevin; The transhipment of participation intracellular protein: CG17248 is directly to homologue	402
RNAi-pMON97133：001	Dv.113; Unknown Function; The WCR unique sequences	422
			RNAi-pMON97121：001	Dv.122_CG3164; The ATP-binding cassette translocator activity of inferring: CG3164 is directly to homologue	454
RNAi-pMON97134：001	Dv.127; Unknown Function; Do not have homology with the people	470
			RNAi-pMON97171：001	Dv.146; Unknown Function, the WCR unique sequences	514
RNAi-pMON97166：001	Dv.147; Unknown Function, the WCR unique sequences	518
			RNAi-pMON97167：001	Dv.149; Unknown Function, the WCR unique sequences	526
RNAi-pMON97169：001	Dv.155; Unknown Function, the WCR unique sequences	550
			RNAi-pMON97173：001	Dv.162; Unknown Function, the WCR unique sequences	578
RNAi-pMON97170：001	Dv.170; Unknown Function, the WCR unique sequences	610

Table 2: adopting feeding of western corn rootworm (WCR) larva to raise the dsRNA construct that causes remarkable hypoevolutism level in the biological assay

Carrier	Be expressed as the sequence of dsRNA
		RNAi-pIC17553：001	Apple
RNAi-pIC17504：049	EST total length V-ATP enzyme
		RNAi-pIC17554：001	Rpl 9
RNAi-pIC17555：001	Rpl 19
		RNAi-pIC17504：050	6.0 part of V-ATP enzyme
RNAi-pIC16005：001	V-ATP enzyme D subunit 1
		RNAi-pIC19514：001	WCR mRNA capping enzyme LIB5496-028-A1-M1-A7
RNAi-pIC17546：001	Dv.1_CG6217
		RNAi-pIC17549：001	Dv.4_CG1435
RNAi-pIC17551：001	LIB5462-012-A1-M2-B2 Dv.5_cg1915_2
		RNAi-pIC17552：001	Dv.6_CG9355
RNAi-pMON78412：001	Dv.7_CG3416
		RNAi-pMON78412：002	Dv.7_CG3416; The Mov34:CG3416 that infers is directly to homologue
RNAi-pMON96172：002	Dv.8_CG1088; The vacuole H+ATP enzyme E subunit of inferring: CG1088 is directly to homologue
		RNAi-pMON96168：002	Dv.9_CG2331; The atpase activity of inferring: CG2331 is directly to homologue
RNAi-pMON78424：002	Dv.10_CG6141; The ribosomal protein L 9:CG6141 that infers is directly to homologue
		RNAi-pMON78425：001	Dv.11_CG2746
RNAi-pMON78444：002	Dv.12_CG1341; The proteasome of inferring is regulated particle, and rpt1:CG1341 is directly to homologue
		RNAi-pMON78416：002	Dv.13_CG11276; The ribosome protein S 4-9 of inferring: CG11276 is directly to homologue
RNAi-pMON78434：001	Dv.14_CG179272; The myoglobulin heavy chain of inferring: CG17927 is directly to homologue

RNAi-pMON78439：001	Dv.16_CG5394; Glutamy-the Alanyl-tRNA synthetase of inferring: CG5394 is directly to homologue
		RNAi-pMON78435：002	Dv.18_CG1404; The RAN minor comonomer GTP enzyme of inferring: CG1404 is directly to homologue
RNAi-pMON78449：002	Dv.19_CG18174; The proteasome of inferring is regulated particle, covers inferior mixture, and rpn11:CG18174 is directly to homologue
		RNAi-pMON78440：002	Dv.20_CG3180_2; The rna plymerase ii that is oriented to DNA of inferring: CG3180 is directly to homologue
RNAi-pMON78420：002	Dv.21_CG3320; The Rab1:CG3320 that infers is directly to homologue
		RNAi-pMON78410：001	Dv.22_CG3395; The ribosomal protein S9:CG3395 that infers is directly to homologue
RNAi-pMON78422：001	Dv.23_CG7269; The helicase of inferring: CG7269 is directly to homologue
		RNAi-pMON78423：001	Dv.25_CG9012; The clathrin heavy chain of inferring: CG9012 is directly to homologue
RNAi-pMON78414：001	Dv.26_CG9261; The sodium of inferring/potassium exchange ATP enzyme: CG9261 is directly to homologue
		RNAi-pMON78413：001	Dv.27_CG12052; The rna plymerase ii transcription factor of inferring: CG12052 is directly to homologue.
RNAi-pMON97122：001	C1_Dv.35; The Vha68-2:CG3762 that infers is directly to homologue; Concatermer
		RNAi-pMON97127：001	C2_Dv.35; The Vha68-2:CG3762 that infers is directly to homologue; Concatermer
RNAi-pMON97125：001	C3_Dv.35; The Vha68-2:CG3762 that infers is directly to homologue; Concatermer
		RNAi-pMON78427：007	Dv.35_CG3762; The Vha68-2:CG3762 that infers is directly to homologue.
RNAi-pMON78441：001	Dv.39_CG9078; Sphingolipid δ-4 desaturase of inferring; Stearyl-CoA 9-desaturase: CG9078 is directly to homologue

RNAi-pMON97114：001	Dv.41_CG2637; Female sterile Ketel; Participating in albumen-nucleus enters: CG2637 is directly to homologue
		RNAi-pMON78429：001	Dv.46_CG10689; The rna helicase enzyme of inferring: CG10689 is directly to homologue.
RNAi-pMON78432：001	Dv.48_CG33196; The transmembrane receptor protein Tyrosylprotein kinase of inferring: CG33196 is directly to homologue
		RNAi-pMON78428：001	Dv.49_CG8055_1; The combination of inferring, carrier activity: CG8055_1 is directly to homologue
RNAi-pMON78428：003	Dv.49_CG8055_1; The combination of inferring, carrier activity: CG8055_1 directly is selected from the free district of DV.49 to homologue
		RNAi-pMON78426：001	Dv.50_CG10110_1; Cutting of inferring and polyadenylation atopen: CG10110_1 are directly to homologue
RNAi-pMON96185：001	Dv.55_CG5931; The splicing factor activity of inferring, rna helicase enzymic activity: CG5931 is directly to homologue
		RNAi-pMON78442：001	Dv.57_CG2968; The hydrogen output ATP enzyme of inferring: CG2968 is directly to homologue
RNAi-pMON78431：001	Dv.58_CG1751; The signal peptidase of inferring: CG1751 is directly to homologue
		RNAi-pMON96177：001	Dv.61_CG3725_1; The calcium atpase of inferring: CG3725 is directly to homologue
RNAi-pMON96182：001	Dv.61_CG3725_2; The calcium atpase of inferring: CG3725 is directly to homologue
		RNAi-pMON96183：001	Dv.62_CG3612; The lead of inferring: CG3612 is directly to homologue
RNAi-pMON96180：002	Dv.65_CG7033; The chaperone activity of inferring: CG7033 is directly to homologue
		RNAi-pMON96180：001	Dv.65_CG7033; The chaperone activity of inferring: CG7033 is directly to homologue
RNAi-pMON96176：001	Dv.66_CG32019; The tendency of inferring: CG32019 is directly to homologue

RNAi-pMON96170：002	Dv.67_CG16916; The endopeptidase activity of inferring: CG16916 is directly to homologue
		RNAi-pMON96166：001	Dv.70_CG5771; The Rab-protein 11 of inferring: CG5771 is directly to homologue
RNAi-pMON96179：001	Dv.72_CG6831; The rhea:CG6831 that infers is directly to homologue
		RNAi-pMON96186：001	Dv.73_CG10119; The lamin C:CG10119 that infers is directly to homologue
RNAi-pMON96160：002	Dv.74_CG6375; The pitchoune:CG6375 that infers is directly to homologue
		RNAi-pMON96160：001	Dv.74_CG6375; The pitchoune:CG6375 that infers is directly to homologue
RNAi-pMON97137：002	Dv.77_CG4214; The syntaxin 5 of inferring; The transhipment of participation intracellular protein: CG4214 is directly to homologue
		RNAi-pMON96167：001	Dv.82_CG8264; The Bx42:CG8264 that infers is directly to homologue
RNAi-pMON96171：001	Dv.83_CG11397; The gluon:CG11397 that infers is directly to homologue
		RNAi-pMON96187：003	Dv.85_CG4494; The protein binding activity of inferring: CG4494 is directly to homologue
RNAi-pMON97126：001	Dv.88_CG8756; Unknown Function; Contain chitin binding domains: CG8756 directly to homologue
		RNAi-pMON97109：001	Dv.99_CG2446; Unknown; Cause death in fruit bat and have low homology with the people: CG2446 is directly to homologue
RNAi-pMON97111：001	Dv.105_CG1250_1; The GTP zymoexciter, participate in the intracellular protein transhipment: CG1250 is directly to homologue
		RNAi-pMON97107：001	Dv.107_CG14813; COPI vacuole tunicle; Participate in the intracellular protein transhipment of golgi body to endoplasmic reticulum: CG14813 is directly to homologue
RNAi-pMON97115：001	Dv.108_CG17248; N-synaptobrevin; The transhipment of participation intracellular protein: CG17248 is directly to homologue

RNAi-pMON97121：001	Dv.122_CG3164; The ATP-binding cassette translocator activity of inferring: CG3164 is directly to homologue
		RNAi-pMON97171：001	Dv.146; Unknown Function, the WCR unique sequences
RNAi-pMON97166：001	Dv.147; Unknown Function, the WCR unique sequences
		RNAi-pMON97167：001	Dv.149; Unknown Function, the WCR unique sequences
RNAi-pMON97169：001	Dv.155; Unknown Function, the WCR unique sequences
		RNAi-pMON97173：001	Dv.162; Unknown Function, the WCR unique sequences
RNAi-pMON97170：001	Dv.170; Unknown Function, the WCR unique sequences

Table 3: adopting feeding of western corn rootworm (WCR) larva to raise the dsRNA construct that causes remarkable dead level in the biological assay

Carrier	Be expressed as the sequence of dsRNA
		RNAi-pIC17553：001	Apple
RNAi-pIC17504：049	EST total length V-ATP enzyme
		RNAi-pIC17555：001	Rpl 19
RNAi-pIC17554：001	Rpl 9
		RNAi-pIC17504：050	6.0 part of V-ATP enzyme
RNAi-pIC17504：054	From V-ATP enzyme subunit 2 sequences of Diabrotica virgifera virgifera total length est sequence as positive control
		RNAi-pIC19514：001	WCR mRNA capping enzyme LIB5496-028-A1-M1-A7
RNAi-pIC17546：001	Dv.1_CG6217
		RNAi-pIC17549：001	Dv.4_CG1435
RNAi-pIC17550：001	LIB5444-065-A1-M1-D5 Dv.5_cg1915_1
		RNAi-pMON78412：002	Dv.7_CG3416; The Mov34:CG3416 that infers is directly to homologue
RNAi-pMON96172：001	Dv.8_CG1088; The vacuole H+ATP enzyme E subunit of inferring: CG1088 is directly to homologue
		RNAi-pMON96168：001	Dv.9_CG2331; The atpase activity of inferring: CG2331 is directly to homologue

RNAi-pMON78424：002	Dv.10_CG6141; The ribosomal protein L 9:CG6141 that infers is directly to homologue
		RNAi-pMON78425：001	Dv.11_CG2746
RNAi-pMON78444：001	Dv.12_CG1341; The proteasome of inferring is regulated particle, and rpt1:CG1341 is directly to homologue
		RNAi-pMON78416：002	Dv.13_CG11276; The ribosome protein S 4-9 of inferring: CG11276 is directly to homologue
RNAi-pMON78434：001	Dv.14_CG17927_2; The myoglobulin heavy chain of inferring: CG17927 is directly to homologue
		RNAi-pMON78435：001	Dv.18_CG1404; The RAN minor comonomer GTP enzyme of inferring: CG1404 is directly to homologue
RNAi-pMON78449：001	Dv.19_CG18174; The proteasome of inferring is regulated particle, covers inferior mixture, and rpn11:CG18174 is directly to homologue
		RNAi-pMON78440：001	Dv.20_CG3180; The rna plymerase ii that is oriented to DNA of inferring: CG3180 is directly to homologue
RNAi-pMON78420：001	Dv.21_CG3320; The Rab1:CG3320 that infers is directly to homologue
		RNAi-pMON78410：001	Dv.22_CG3395; The ribosomal protein S9:CG3395 that infers is directly to homologue
RNAi-pMON78422：001	Dv.23_CG7269; The helicase of inferring: CG7269 is directly to homologue
		RNAi-pMON78414：006	5 ' part of the about EST of DV.26sec1
RNAi-pMON78414：001	Dv.26_CG9261; The sodium of inferring/potassium exchange ATP enzyme: CG9261 is directly to homologue
		RNAi-pMON97122：001	C1_Dv.35; The Vha68-2:CG3762 that infers is directly to homologue; Concatermer
RNAi-pMON97127：001	C2_Dv.35; The Vha68-2:CG3762 that infers is directly to homologue; Concatermer
		RNAi-pMON97125：001	C3_Dv.35; The Vha68-2:CG3762 that infers is directly to homologue; Concatermer

RNAi-pMON78427：001	Dv.35_CG3762; The Vha68-2:CG3762 that infers is directly to homologue.
		RNAi-pMON97114：001	Dv.41_CG2637; Female sterile Ketel; Participating in albumen-nucleus enters: CG2637 is directly to homologue
RNAi-pMON97140：001	Dv.44_CG1244; The nucleic acid of inferring in conjunction with activity: CG1244 directly to homologue
		RNAi-pMON78429：001	Dv.46_CG10689; The rna helicase enzyme of inferring: CG10689 is directly to homologue.
RNAi-pMON78428：001	Dv.49_CG8055_1; The combination of inferring, carrier activity: CG8055_1 is directly to homologue
		RNAi-pMON78428：003	Dv.49_CG8055_1; The combination of inferring, carrier activity: CG8055_1 directly is selected from the free district of DV.49 to homologue
RNAi-pMON78426：001	Dv.50_CG10110_1; Cutting of inferring and polyadenylation atopen: CG10110_1 are directly to homologue
		RNAi-pMON96185：001	Dv.55_CG5931; The splicing factor activity of inferring, rna helicase enzymic activity: CG5931 is directly to homologue
RNAi-pMON78431：001	Dv.58_CG1751; The signal peptidase of inferring: CG1751 is directly to homologue
		RNAi-pMON96177：001	Dv.61_CG3725_1; The calcium atpase of inferring: CG3725 is directly to homologue
RNAi-pMON96182：001	Dv.61_CG3725_2; The calcium atpase of inferring: CG3725 is directly to homologue
		RNAi-pMON96180：001	Dv.65_CG7033; The chaperone activity of inferring: CG7033 is directly to homologue
RNAi-pMON96176：001	Dv.66_CG32019; The tendency of inferring: CG32019 is directly to homologue
		RNAi-pMON96170：001	Dv.67_CG16916; The endopeptidase activity of inferring: CG16916 is directly to homologue
RNAi-pMON96166：001	Dv.70_CG5771; The Rab-protein 11 of inferring: CG5771 is directly to homologue

RNAi-pMON96160：001	Dv.74_CG6375; The pitchoune:CG6375 that infers is directly to homologue
		RNAi-pMON97137：001	Dv.77_CG4214; The syntaxin 5 of inferring; The transhipment of participation intracellular protein: CG4214 is directly to homologue
RNAi-pMON96167：001	Dv.82_CG8264; The Bx42:CG8264 that infers is directly to homologue
		RNAi-pMON96187：002	Dv.85_CG4494; The protein binding activity of inferring: CG4494 is directly to homologue
RNAi-pMON97126：001	Dv.88_CG8756; Unknown Function; Contain chitin binding domains: CG8756 directly to homologue
		RNAi-pMON97130：001	Dv.93_CG8515; The epidermal structure component of inferring; Contain chitin binding domains: CG8515 directly to homologue
RNAi-pMON97111：001	Dv.105_CG1250_1; The GTP zymoexciter, participate in the intracellular protein transhipment: CG1250 is directly to homologue
		RNAi-pMON97107：001	Dv.107_CG14813; COPI vacuole tunicle; Participating in golgi body transports to the endoplasmic reticulum intracellular protein: CG14813 is directly to homologue
RNAi-pMON97115：001	Dv.108_CG17248; N-synaptobrevin; The transhipment of participation intracellular protein: CG17248 is directly to homologue
		RNAi-pMON97133：001	Dv.113; Unknown Function; The WCR unique sequences
RNAi-pMON97121：001	Dv.122_CG3164; The ATP-binding cassette translocator activity of inferring: CG3164 is directly to homologue
		RNAi-pMON97134：001	Dv.127; Unknown Function; There is not homology with the people

Table 4: adopting feeding of southern corn rootworm (SCR) larva to raise the dsRNA construct that causes remarkable hypoevolutism level in the biological assay

Carrier	Be expressed as the sequence of dsRNA
		RNAi-pMON96172：001	Dv8_CG1088; The vacuole H+ATP enzyme E subunit of inferring: CG1088 is directly to homologue
RNAi-pMON96168：001	Dv9_CG2331; The atpase activity of inferring: CG2331 is directly to homologue
		RNAi-pMON78424：001	Dv.10_CG6141
RNAi-pMON96155：002	Dv.10_CG6141; The ribosomal protein L 9:CG6141 that infers is directly to homologue.
		RNAi-pMON78425：001	Dv.11_CG2746
RNAi-pMON96158：002	Dv.11_CG2746; The ribosomal protein L 19 of inferring: CG2746 is directly to homologue.
		RNAi-pMON78416：001	Dv.13_CG11276
RNAi-pMON78434：001	Dv.14_CG17927_2; The myoglobulin heavy chain of inferring: CG17927 is directly to homologue
		RNAi-pMON78435：001	Dv.18_CG1404; The RAN minor comonomer GTP enzyme of inferring: CG1404 is directly to homologue
RNAi-pMON78449：001	Dv.19_CG18174; The proteasome of inferring is regulated particle, covers inferior mixture, and rpn11:CG18174 is directly to homologue
		RNAi-pMON78419：001	Dv.20_CG3180
RNAi-pMON78420：001	Dv.21_CG3320; The Rab1:CG3320 that infers is directly to homologue
		RNAi-pMON78414：001	Dv.26_CG9261; The sodium of inferring/potassium exchange ATP enzyme: CG9261 is directly to homologue
RNAi-pMON97122：001	C1_Dv35; The Vha68-2:CG3762 that infers is directly to homologue; Concatermer
		RNAi-pMON97125：001	C3_Dv35; The Vha68-2:CG3762 that infers is directly to homologue; Concatermer

RNAi-pMON78427：008	Dv.35_CG3762; The Vha68-2:CG3762 that infers is directly to homologue.
		RNAi-pMON78428：001	Dv.49_CG8055_1; The combination of inferring, carrier activity: CG8055_1 is directly to homologue
RNAi-pMON78442：001	Dv.57_CG2968; The hydrogen output ATP enzyme of inferring: CG2968 is directly to homologue
		RNAi-pMON96177：001	Dv61_CG37251; The calcium atpase of inferring: CG3725 is directly to homologue
RNAi-pMON96166：001	Dv70_CG5771; The Rab-protein 11 of inferring: CG5771 is directly to homologue
		RNAi-pMON97126：001	Dv88_CG8756; Unknown Function; Contain chitin binding domains: CG8756 directly to homologue
RNAi-pMON97111：001	Dv105_CG1250_1; The GTP zymoexciter, participate in the intracellular protein transhipment: CG1250 is directly to homologue
		RNAi-pMON97112：001	Dv105_CG1250_2; The GTP zymoexciter, participate in the intracellular protein transhipment: CG1250 is directly to homologue
RNAi-pMON97107：001	Dv107_CG14813; COPI vacuole tunicle; Participate in the intracellular protein transhipment of golgi body to endoplasmic reticulum: CG14813 is directly to homologue
		RNAi-pMON97121：001	Dv122_CG3164; The ATP-binding cassette translocator activity of inferring: CG3164 is directly to homologue

Table 5: adopting feeding of southern corn rootworm (SCR) larva to raise the dsRNA construct that causes remarkable dead level in the biological assay

Carrier	Be expressed as the sequence of dsRNA
		RNAi-pIC17546：003	LIB5462-091-A1-M1-G3 Dv1_CG6217 KNICKKOPF UNK
RNAi-pIC17504：055	From V-ATP enzyme subunit 2 sequences of Diabrotica virgifera virgifera total length est sequence as positive control

RNAi-pMON96155：001	Dv.10_CG6141; The ribosomal protein L 9:CG6141 that infers is directly to homologue
		RNAi-pMON96158：001	Dv.11_CG2746; The ribosomal protein L 19 of inferring: CG2746 is directly to homologue
RNAi-pMON78416：001	Dv.13_CG11276
		RNAi-pMON96154：003	Dv.14_CG17927; The myoglobulin heavy chain of inferring: CG17927 is directly to homologue; The cell of in the S perfect medium, growing
RNAi-pMON78438：001	Dv.17_CG10149; The proteasome p44.5 subunit of inferring, rpn6:CG10149 is directly to homologue
		RNAi-pMON78449：001	Dv.19_CG18174; The proteasome of inferring is regulated particle, covers inferior mixture, and rpn11:CG18174 is directly to homologue
RNAi-pMON78440：001	Dv.20_CG3180; The rna plymerase ii that is oriented to DNA of inferring: CG3180 is directly to homologue
		RNAi-pMON96156：001	Dv.20_CG3180; The rna plymerase ii 140kD subunit of inferring: CG3180 is directly to homologue
RNAi-pMON78420：001	Dv.21_CG3320; The Rab1:CG3320 that infers is directly to homologue
		RNAi-pMON78427：006	Dv.35_CG3762; The Vha68-2:CG3762 that infers is directly to homologue.
RNAi-pMON78428：001	Dv.49_CG8055_1; The combination of inferring, carrier activity: CG8055_1 is directly to homologue
		RNAi-pMON96177：001	Dv61_CG3725_1; The calcium atpase of inferring: CG3725 is directly to homologue
RNAi-pMON96166：001	Dv70_CG5771; The Rab-protein 11 of inferring: CG5771 is directly to homologue
		RNAi-pMON96174：001	Dv86_CG5055; The bazooka:CG5055 that infers is directly to homologue
RNAi-pMON97111：001	Dv105_CG1250_1; The GTP zymoexciter, participate in the intracellular protein transhipment: CG1250 is directly to homologue

RNAi-pMON97107：001

Dv107_CG14813; COPI vacuole tunicle; Participate in the intracellular protein transhipment of golgi body to endoplasmic reticulum: CG14813 is directly to homologue

Embodiment 4

Transgenic plant transform and biological assay

In brief, the sequence of above-mentioned coding dsRNA construct at 5 ' end and link to each other by being operatively connected the sequence that constitutes in the 35S promoter of corn hsp70 intron, is held 3 ' to link to each other with poly-adenosine sequence with NOS3 ' Transcription Termination.Place careless glycosides phosphine to select the downstream of box this expression cassette.Then these boxes that link to each other are placed Agrobacterium tumefaciens Plant Transformation function carrier, be used for the maize transformation tissue, make it have careless glycosides phosphine tolerance, the selection incident shifts soil.R ₀The velamen of plant is fed and is raised to western corn rootworm larva (WCR, Diabrotica virifera).The transgenic corns root is transferred on the culture dish, had the MS0D substratum that contains microbiotic and careless glycosides phosphine on it, carry out external selection.Make each root in each ware be subjected to the infection of two WCR larvas with thin paintbrush.(Parafilm) seals these wares with Parafilm, prevents the larva escape.Test is carried out under complete dark in 60% the RH Percival incubator at 27 ℃.Pollution and larva quality are monitored.Feed raise root tissue six days after, larva is transferred in the WCR food in 96 orifice plates.Larva be allowed to ingest this food eight days makes that total fortnight that is determined as is long.To the larva quality and the in addition record of surviving, be used for analyzing.The larva conceptual data is carried out single factor ANOVA analysis and Dunnett check, seek the significance,statistical of comparing with unconverted negative control.On two kinds of incidents, feed and raise the back to measure larvae development slow, and compare with the growth of feeding the larva of raising the negative control plant.

With transgenic corn plant (R ₀) plant in 10 inches jars that contain Metromix soil.When plant reaches the V4 growth phase, make about 1000 western corn rootworms (WCR, Diabrotica virifera) ovum attack into root area.Same genotypic non-transgenic corn is attacked at similar growth phase, to be used as negative control.Ovum is carried out preincubate, make and in 24 hours of invasion and attack, to hatch.Larva is allowed to ingest for 3 weeks at root system.Remove plant from soil, it is cleaned, making can be at larva feed assessment root.Use tubercle damage scoring (NIS) in addition classification of root damage, wherein not damage of 0 expression, a joint of 1 expression root has been lacked in 1.5 inches, and 2 expressions, two joints are short, and 3 expressions, 3 joints are short.Because the plant that is used to evaluate and test be transform after directly from tissue culture, also because transformation event is unique,, can not obtain statistics so only evaluate and test the individual plant plant at every kind of incident this moment.All plant in the mensuration have all shown sign and symptom that larva ingests, and this shows and has obtained successful invasion and attack.The negative control plant on average be moderate to major injury, and the root of transgenic plant provides the remarkable control that larva is ingested, tubercle damage scoring is about 0.2 or littler.

Embodiment 5

Silencing methods realization insect pest gene with the ta-siRNA mediation is prevented

Make a kind of alternative method of gene silencing in the plant insect adopt a class trans-acting siRNA (ta-siRNA) (people such as Dalmay, 2000 of recent findings; People such as Mourrain, 2000; People such as Peragine, 2004; People such as Vazquez, 2004).Ta-siRNA is that the single stranded RNA transcript of naturally occurring miRNA target from cell obtains.Use microRNA to be used for being described in the U.S. Provisional Patent Application sequence number 60/643,136 people 2004 such as () Carrington in the method that plant carries out gene silencing to bring out ta-siRNA, its by reference integral body incorporate this paper into.Identified at least a insect specificity miRNA that in corn rootworm larva intestinal epithelial cell, expresses.Identify at least a target rna transcription thing sequence, the miRNA complementation of expressing in described sequence and the cell with this insect specificity miRNA then.Corresponding target sequence is the short sequence that is no more than 21 adjacent nucleotides, when in the cell type with functional r NAi approach during as the part of rna transcription thing and the miRNA contact corresponding with it, described short sequence causes the cutting to described transcript of cut substrate mediation.In case identified the miRNA target sequence, at least a miRNA target sequence and second sequence are merged, described second sequence is corresponding to using this method to carry out the part of reticent insect gene.For example, with arbitrary sequence or its part among miRNA target sequence and the SEQ ID NO:1-SEQ ID NO:906, for example the sequence of corn rootworm vacuole ATP enzyme (V-ATP enzyme) gene merges.The miRNA target sequence can place 5 ' end of V-ATP enzyme gene, and 3 ' holds or be embedded in the middle of this gene.Use is perhaps repeatedly used in the mosaic of miRNA target sequence and target-gene sequence with a kind of miRNA target sequence corresponding to the multiple miRNA target sequence of multiple miRNA gene, may be preferred.Target-gene sequence can be a random length, but minimum is 21bp.

Use any a large amount of suitable promotor or other transcription regulatory element, the mosaic of miRNA target sequence target-gene sequence is expressed in the vegetable cell, get final product as long as can transcribe at the cell type that will be provided in pest food (for example, being used for controlling the corn root of corn rootworm).

This method can have additional advantage: can send than long RNA molecule and pass to the target insect.Typically, the dsRNA that produces in plant can easily be processed as short rna by Dicer, when beyond the source side formula feed when raising to some insects, this may be invalid.In the method, produce the strand transcript in vegetable cell, it is absorbed by insect, turns to dsRNA at the insect transit cell, in cell, it is processed into the ta-siRNA that can cause post-transcriptional silencing to one or more genes in one or more target insects again.

Embodiment 6

Be provided for the method for dna sequence dna of the gene silencing of dsRNA mediation

Present embodiment has illustrated the method for the dna sequence dna of the gene silencing that is provided for the dsRNA mediation.More specifically, present embodiment has been described the selection to the improved DNA in the gene silencing that can be used for the dsRNA mediation, and this realizes by following step: (a) select initial dna sequence dna from target gene, it comprises more than 21 adjacent Nucleotide; (b) identify at least one shorter dna sequence dna, it derives from the zone of initial dna sequence, by be predicted to be do not produce undesired polypeptide and with constitute to the zone that the known array of homologue does not have identity such as homologue/directly; And the dna sequence dna of (c) selecting to be used for the gene silencing of dsRNA mediation, it comprises the dna sequence dna that at least one is shorter.Undesired polypeptide includes but not limited to, with the polypeptide of sensitization homologous peptide and with known polypeptide toxin homologous polypeptide.

WCR V-ATP enzyme has proved to feed to raise in the mensuration at corn rootworm and has played a role, with the silence (as the means of control larval growth) of check dsRNA mediation.CDNA sequence from the vacuole ATP enzyme gene (V-ATP enzyme) of western corn rootworm (WCR) (Diabrotica cirgifera virgifera LeConte) is selected, and is used as initial dna sequence dna.Screen initial dna sequence dna, be used for identifying with lower area: in described zone, each adjacent segment that comprises at least 21 Nucleotide only with known vertebrates sequence in 21 adjacent Nucleotide less than 21 couplings.Identified the sequence fragment that surpasses about 100 adjacent nucleotides, wherein do not contained described 21/21 and hit.Therefore, can with comprise fragment length, GC content, sequence, based on the sequence of the corresponding gene in the model biology or function and the standard of the secondary structure of function of predicting and prediction (people such as Elbashir for example 2001b) can be used to select and design the sequence of use.Made up the various combination of these sequence fragments, be used for construction expression and be dsRNA and be used for above-described insect feeding the gomphosis DNA array of raising biological assay.

Embodiment 7

The insect that carries out with the sequence that is selected from est database feeds other result who raises biological assay

Present embodiment has illustrated to be found can effectively cause other slow and/or dead sequence of larvae development as the double-stranded RNA sequence when the rootworm larva attacks.Raise the corn rootworm larva method, dsRNA use description with insect biological assay such as embodiment 1-3.Result of study has confirmed that the larvae development of the conspicuous level (p＜0.05) that causes with the part that contains with multiple different genes type homologous sequence is slow and/or dead.Produce remarkable hypoevolutism and/or dead sequence and carrier, and the corresponding sequence numbering that is expressed as the sequence of dsRNA is shown in following table 6.Being used for exemplary binary vector pMON98503 that corn transforms contains and is useful on right side and the following element between the T-DNA edge, left side: e35S-HSP70-DV49 (antisense orientation)-general spacer DV49 (the sense orientation)-hsp17 that transfers in the vegetable cell; ACT (promotor and intron)-CTP2 transports signal-CP4-NOS.Be used for another kind of exemplary binary vector pMON98504 that corn transforms and contain following element between right side and left side edge: e35S-HSP70-C1 (antisense orientation)-general spacer C1 (sense orientation)-hsp17; ACT (promotor and intron)-CTP2 transports signal-CP4-NOS.

Table 6: the insect that carries out with southern corn rootworm or western corn rootworm feeds to raise and has confirmed remarkable hypoevolutism and/or other dead dsRNA construct in the biological assay

Carrier	Be expressed as the sequence of dsRNA	SEQ ID NO
			pMON98356	Dv164; Unknown Function, the WCR unique sequences	726
pMON98354	Dv172; Unknown Function, the WCR unique sequences	727
			pMON97191	Dv189; Unknown Function, the WCR unique sequences	728
pMON98359	Dv200; Unknown Function, the WCR unique sequences	729
			pMON38880	Dv207_F39H11.5; The pbs-7 that infers, endopeptidase: F39H11.5 is directly to homologue	730
pMON101054	Dv208_F58F12_1; The plastosome F1F0-ATP synthase of inferring, subunit delta/ATP16:F58F12_1 is directly to homologue	731
			pMON98437	Dv210_K11H12_2; The rpl-15 that infers, ribosomal structural constituent: K11H12_2 is directly to homologue	732
pMON98435	Dv211_R12E2_3; The rpn-8 that infers, translation initiation factor, the 26S proteasome is regulated mixture, and subunit RPN8:R12E2_3 is directly to homologue	733
			pMON98447	Dv212_C17H12_14; The vha-8 that infers, hydrogen output ATP enzyme: C17H12_14 is directly to homologue	734

Carrier	Be expressed as the sequence of dsRNA	SEQ ID NO
			pMON98448	Dv213_B0464_1; The drs-1 that infers, tRNA ligase enzyme: B0464_1 is directly to homologue	735
pMON101059	Dv214_F53G12_10; The rpl-7 that infers, ribosomal structural constituent: F53G12_10 is directly to homologue	736
			pMON98442	Dv216_C52E4_4; The rpt-1 that infers, the 19S of proteasome regulate the ATP enzyme subunit C52E4_4 of mixture directly to homologue	737
pMON98441	Dv218_K01G5_4; The ran-1 that infers, minor comonomer GTP enzyme: K01G5_4 is directly to homologue	738
			pMON98440	Dv219_C15H11_7; The pas-1 that infers, endopeptidase: C15H11_7 is directly to homologue	739
pMON101081	Dv223_R10E11.1; The cbp-1 that infers, the homologue of transcriptional coactivator CBP and p300: R10E11.1 is directly to homologue	740
			pMON101050	Dv224_F11C3_3; The unc-54 that infers, the ATP combination; Motorius activity: F11C3_3 is directly to homologue	741
pMON101051	Dv225_C37H5_8; The hsp-6 that infers, heat shock protein 6:C37H5_8 is directly to homologue	742
			pMON38888	Dv226_C47E12.5; The uba-1 that infers, ubiquitin activates: C47E12.5 is directly to homologue	743
pMON38887	Dv227_F54A3.3; The chaperone mixture component of inferring, TCP-1 γ subunit: F54A3.3 is directly to homologue	744
			pMON101110	Dv229_D1081.8; The Myb-sample DNA combination of inferring: D1081.8 is directly to homologue	745
pMON101052	Dv230_F55A11_2; The syn-3 that infers, protein transport albumen; Syntaxin: F55A11_2 is directly to homologue	746
			pMON101107	Dv231_C30C11.1; The mitochondrial ribosomal protein L32:C30C11.1 that infers is directly to homologue	747
pMON101055	Dv232_B0250_1; The rpl-2 that infers, ribosomal structural constituent: B0250_1 is directly to homologue	748

Carrier	Be expressed as the sequence of dsRNA	SEQ ID NO
			pMON98446	Dv233_F54C9_5; The rpl-5 that infers, the 5SrRNA combination, ribosomal structural constituent: F54C9_5 is directly to homologue	749
pMON101138	Dv235_C04F12.4; The rpl-14 that infers, big ribosomal subunit L14 albumen: C04F12.4 is directly to homologue	750
			pMON98449	Dv236_C01G8_5; The erm-1 that infers, ezrin/radicin/moesin (ERM) cytoskeleton linker family: C01G8_5 is directly to homologue	751
pMON98439	Dv237_F57B9_10; The rpn-6 that infers, proteasome is regulated particle, and non--ATP enzyme-sample: F57B9_10 is directly to homologue	752
			pMON98436	Dv240_F53A3_3; The rps-22 that infers, ribosomal structural constituent: F53A3_3 is directly to homologue	753
pMON101078	Dv241_F32H2.5; The alcoholdehydrogenase of inferring, zinc is dependent: F32H2.5 is directly to homologue	754
			pMON101058	Dv242_B0336_2; The arf-1 that infers, minor comonomer GTP enzyme: B0336_2 is directly to homologue	755
pMON101057	Dv244_C14B9_7; The rpl-21 that infers, ribosomal structural constituent: C14B9_7 is directly to homologue	756
			pMON98444	Dv245_C26F1_4; The rps-30 that infers, ribosomal protein, small subunit: C26F1_4 is directly to homologue	757
pMON98434	Dv247_C13B9_3; The δ subunit of the coatmer of inferring (COPI) mixture: C13B9_3 is directly to homologue	758
			pMON101053	Dv248_F38E11_5; The vacuole of inferring is outer by mixture COPI, and β ' subunit: F38E11_5 is directly to homologue	759
pMON98445	Dv249_F37C12_9; The rps-14 that infers, ribosomal structural constituent: F37C12_9 is directly to homologue	760
			pMON101056	Dv250_CD4_6; The pas-6 that infers, endopeptidase: CD4_6 is directly to homologue	761
pMON101104	Dv251_D1007.12; The rpl-24.1 that infers, ribosomal structural constituent: D1007.12 is directly to homologue	762

Carrier	Be expressed as the sequence of dsRNA	SEQ ID NO
			pMON101088	Dv252_C49H3.11; The rps-2 that infers, ribosomal structural constituent: C49H3.11 is directly to homologue	763
pMON101079	Dv253_C26D10.2; The hel-1 that infers, ATP-RNA-dependent helicase: C26D10.2 is directly to homologue	764
			pMON101085	Dv254_B0336.10; The rpl-23 that infers, ribosomal structural constituent: B0336.10 is directly to homologue	765
pMON38879	Dv255_C36A4.2; The Cytochrome P450 family member who infers: C36A4.2 is directly to homologue	766
			pMON101087	Dv256_K05C4.1; The pbs-5 that infers, proteasome beta subunit: K05C4.1 is directly to homologue	767
pMON101082	Dv257_F29G9.5; The rpt-2 that infers, 26S proteasome regulate mixture: F29G9.5 directly to homologue	768
			pMON101084	Dv258_F40F8.10; The rps-9 that infers, ribosomal structural constituent: F40F8.10 is directly to homologue	769
pMON101083	Dv259_K07D4.3; The rpn-11 that infers, the 26S proteasome is regulated mixture, and subunit RPNI1:K07D4.3 is directly to homologue	770
			pMON101080	Dv260_F49C12.8; The rpn-7 that infers, proteasome is regulated particle, and non--ATP enzyme-sample: F49C12.8 is directly to homologue	771
pMON101115	Dv261_D1054.2; The pas-2 that infers, endopeptidase: D1054.2 is directly to homologue	772
			pMON101141	Dv263_F55A3.3; The metal exopeptidase of inferring: F55A3.3 is directly to homologue	773
pMON101126	Dv264_F56F3.5; The rps-1 that infers, ribosomal structural constituent: F56F3.5 is directly to homologue	774
			pMON101133	Dv266_C09D4.5; The rpl-19 that infers, ribosomal structural constituent: C09D4.5 is directly to homologue	775
pMON38881	Dv268_R06A4.9; The polyadenylation factor I mixture of inferring, subunit PFS2:R06A4.9 is directly to homologue	776

Carrier	Be expressed as the sequence of dsRNA	SEQ ID NO
			pMON101135	Dv271_F37C12.4; The rpl-36 that infers, ribosomal structural constituent: F37C12.4 is directly to homologue	777
pMON101132	Dv273_F54E7.2; The rps-12 that infers, ribosomal structural constituent: F54E7.2 is directly to homologue	778
			pMON101139	Dv274_C23G10.4; The rpn-2 that infers, proteasome is regulated particle, and non--ATP enzyme-like:C23G10.4 is directly to homologue	779
pMON101130	Dv275_C03D6.8; The rpl-24.2 that infers, ribosomal structural constituent: C03D6.8 is directly to homologue	780
			pMON101119	Dv276_C26E6.4; The RNA polymerase that is oriented to DNA of inferring: C26E6.4 is directly to homologue	781
pMON101134	Dv277_R13A5.8; The rpl-9 that infers, ribosomal structural constituent: R13A5.8 is directly to homologue	782
			pMON101127	Dv279_F42C5.8; The rps-8 that infers, ribosomal structural constituent: F42C5.8 is directly to homologue	783
pMON101122	Dv280_F13B10.2; The rpl-3 that infers, big ribosomal subunit L3:F13B10.2 is directly to homologue	784
			pMON101116	Dv281_T05C12.7; The cct-1 that infers, chaperone mixture component, TCP-1alpha subunit: T05C12.7 is directly to homologue	785
pMON101125	Dv282_F07D10.1; The rpl-11.2 that infers, ribosomal structural constituent: F07D10.1 is directly to homologue	786
			pMON38883	Dv283_T05H4.6; The peptide chain release factor 1 (eRF1) of inferring: Dv283_T05H4.6 is directly to homologue	787
pMON101124	Dv284_C47E8.5; The daf-21 that infers, heat shock 90 albumen, chaperone activity: C47E8.5 is directly to homologue	788
			pMON101120	Dv285_M03F4.2; The act-4 that infers, Actin muscle: M03F4.2 is directly to homologue	789
pMON101137	Dv286_F25H5.4; The eft-2 that infers, translation elongation factor: F25H5.4 is directly to homologue	790

Carrier	Be expressed as the sequence of dsRNA	SEQ ID NO
			pMON101140	Dv287_F26D10.3; The hsp-1 that infers, heat shock protein: F26D10.3 is directly to homologue	791
pMON101117	Dv288_F28D1.7; The rps-23 that infers, ribosomal structural constituent: F28D1.7 is directly to homologue	792
			pMON38886	Dv290_CG11979; The H-output ATP enzyme of inferring: CG11979 is directly to homologue	793
pMON38885	Dv291_CG13628; The H-output ATP enzyme of inferring: CG13628 is directly to homologue	794
			pMON101103	Dv293_CG31237; The rna plymerase ii that is oriented to DNA of inferring: CG31237 is directly to homologue	795
pMON101096	Dv294_CG8669; The cryptocephal that infers; Transcription factor participates in moulting cycle, and nymphosis and metamorphosis: CG8669 is directly to homologue	796
			pMON101095	Dv295_CG8048; The vacuole H+ATP enzyme 44kD C subunit of inferring: CG8048 is directly to homologue	797
pMON101100	Dv298_CG9032; The H-output ATP enzyme of inferring: CG9032 is directly to homologue	798
			pMON101111	Dv299_CG17369; The H-output ATP enzyme of inferring: CG17369 is directly to homologue	799
pMON101129	Dv303_CG4152; The ATP-RNA-dependent helicase of inferring: CG4152 is directly to homologue	800
			pMON101136	Dv305_CG4916; The ATP-RNA-dependent helicase of inferring: CG4919 is directly to homologue	801
pMON101131	Dv315_CG9160; The nadh dehydrogenase of inferring: CG9160 is directly to homologue	802
			pMON101123	Dv316_CG8764; Panthenol-the cytopigment of inferring-c reductase enzyme: CG8764 is directly to homologue	803
pMON98364	The C4_Dv49_CG8055 concatermer; The combination of inferring, carrier activity: CG8055 is directly to homologue	804

Carrier	Be expressed as the sequence of dsRNA	SEQ ID NO
			pMON98365	The C5_Dv49_CG8055 concatermer; The combination of inferring, carrier activity: CG8055 is directly to homologue	805
pMON98368	The highly effective C6 concatermer of WCR target, about 50%GC standard, by according to 5 '-3 ' be Dv26, Dv49, Dv23, Dv20, Dv13, Dv22, the fragment composition of the order of Dv18	806
			pMON98369	The C7 concatermer of insect specific target, about 50%GC standard, by according to 5 '-3 ' be Dv6, Dv1, Dv88, Dv93, Dv4, Dv113, Dv127, the fragment of the order of Dv99 is formed	807
pMON98372	The C8 concatermer; The sodium of inferring/potassium exchange ATP enzyme: CG9261 is directly to homologue	808
			pMON98373	The C9 concatermer; The sodium of inferring/potassium exchange ATP enzyme: CG9261 is directly to homologue	809
pMON98366	C10 concatermer with gene of the identical/different binding modes of inferring, about 50%GC standard	810
			pMON98367	The highly effective C12 concatermer of WCR target, about 50%GC standard, by according to 5 '-3 ' be that the fragment of the order of Dv23-Dv13-Dv26-Dv18-Dv49-Dv22-Dv20 is formed	811
pMON98371	The C14 concatermer of activated gene target in different biologies, about 50% GC standard	812
			pMON98503	The DV49 that comprises from Diabrotica virgifera infers ESCRT-III (the required interior body sorting mixture of transhipment III) complex subunit	820
pMON98504	Comprise the Vha68-2:CG3762 that infers directly to homologue; 250bp concatermer C1;	821
			pMON102862	Dv319_CG14750; The ESCRTII that infers, Vps25:CG14750 are directly to homologue	835
pMON102863	Dv320_CG9712; The ESCRTI that infers, Vps23:CG9712 are directly to homologue	836
			pMON102861	Dv321_CG12770; The ESCRTI that infers, Vps28:CG12770 are directly to homologue	837

Carrier	Be expressed as the sequence of dsRNA	SEQ ID NO
			pMON102865	Dv322_CG14542; The ESCRT III that infers, Vps2:CG14542 is directly to homologue	838
pMON102866	Dv323_CG4071; The ESCRT III that infers, Vsp20:CG4071 is directly to homologue	839
			pMON102871	Dv326_CG3564; The protein carrier of inferring, the component of COPI vacuole tunicle: CG3564 is directly to homologue	840
pMON102873	Dv327_CG6223; The coatmer of inferring, the component of COPI vacuole tunicle: CG6223 is directly to homologue	841
			pMON102877	Dv328_CG6948; The clathrin light-chain of inferring, the outer quilt of the depression of bag quilt: CG6948 is directly to homologue	842
pMON102872	Dv330_CG9543; The COPI vacuole tunicle of inferring: CG9543 is directly to homologue	843
			pMON102879	Dv331_CG5183; The KDEL sequence combination of inferring: CG5183 is directly to homologue	844
pMON102867	Dv335_F11C1.6; The nhr-25 that infers, DNA combination: F11C1.6 is directly to homologue	845
			pMON102870	Dv337_CG18734; The fluorine woods proteolytic enzyme 2 of inferring, serine-type endopeptidase: CG18734 is directly to homologue	846
pMON102875	Dv329_CG7961; The coatmer of inferring, the component of COPI vacuole tunicle: CG7961 is directly to homologue	874

Followingly render a service test, wherein utilize the offspring of maize plant, described maize plant is to transform with selected insect control construct:

1. plant and planted back 7 days: 25 ℃, under the 60%RH under dark fully condition at 10,000 WCR ovum of each incident incubation (10 plant of each incident), incubation 7 days.

2. attacked back 14 days: with plant from 4 " the mud jar is transplanted to 8 " and jar; Can get v4 tip of a root sample and carry out gene expression research.

3. plant and planted back 14 days: the WCR ovum is washed out from soil.Ovum and soil are placed 60 purpose sieves, and one 30 purpose sieve is placed on above the 60 purpose sieves, prevent current with the protection ovum.Adopt nozzle warm water cleaning down, up to removing soil.

4. ovum is suspended in 2% (w/v) Difco agar-agar soln every 1ml ovum 25mL solution.Adopt for example Eppendorf running fire type micropipet,, ovum is attacked in the soil, about 1000 ovum of each plant with about 3 or 4 five equilibriums.Before invasion and attack, in soil, dig a hole, on the invasion and attack bonnet with spatula.

5. plant and planted back 28 days, can take out v8 tip of a root sample and carry out gene expression research.

6. plant and planted back 35 days, assess this mensuration.Cut off plant with pruning scissors, stay about 6 " stem.Burrow in the plant stake that contains event information, knotting (zip tied) is to stem.Remove soil as much as possible from root system.With remaining soil of water jet standpipe flush away.

7. the inspection root damages classification with Olsen (0-3) the NIS standard of WCR larva infringement to root.

The insect control result that Fig. 1 and Fig. 2 explanation obtain with WCR invasion and attack F1 maize plants (deriving from pMON98503 or pMON98504 plant transformed) back.Substantially rendeing a service in the test by the growth room that carries out mentioned above, in the offspring who transforms the incident that obtains with pMON98503 or pMON98504, observing the NIS scoring that is equal to or less than the economic damage threshold value.

To embodiment 3 and the active selected genes assembling of the significantly anti-western corn rootworm of 7 displayings of describing total length EST dna sequence dna.These est sequences are listed in table 7:

Table 7: at the est sequence of WCR target assembling

The sequence of assembling	SEQ ID NO
		Dv9 total length (aka Apple); The CG2331 that infers is directly to homologue	813
Dv10 total length (Rp19); The CG6141 that infers is directly to homologue	814
		Dv11 total length (Rpl19); The CG2746 that infers is directly to homologue	815
Dv13 total length (Rps4); The CG11276 that infers is directly to homologue	816
		Dv35 total length v-ATP enzyme A; CG3762 is directly to homologue	817
The Dv49 total length of CG8055 is directly to homologue	818
		The Dv248 total length of CG6699 is inferred directly to homologue	819

Embodiment 8

The preparation of Dv49 and Dv248 sequence and efficacy results

Based on the phenotype that knocks out mutant in corresponding encoded district in the function that comprises prediction, other biology, fragment length, GC content, with the similarity of known array and the secondary structure of prediction (people such as Elbashir for example, standard 2001b), the part of the EST of selection assembling and the flanking sequence of Dv49 (SEQ IDNO:818) and Dv248 (SEQ ID NO:819) is carried out further biological activity determination.Based on the prediction activity of independent anti-WCR, external synthetic each Dv49 and Dv248 sequence, and according to grouping shown in Fig. 3-4 and the table 8, and be applied to the WCR larva.

Table 8: Dv49 and the Dv248 fragment of rendeing a service assembling at anti-WCR

Fragment	SEQ ID NO
		F1	822
F2	823
		F3	824
F4	825
		F5	826
F6	827
		F7	828
F8	829
		F9	830
F10	831
		F11	832
F12	833
		F13	834

Fragment F1-F3 is corresponding to the part of total length Dv49 transcript.Fragment F4-F6 is corresponding to the part or the flanking region of Dv248 transcript.Fragment F7-F13 is two or more the segmental concatermers among the fragment F1-F6, as shown in Figure 4.Fragment F13 (SEQ ID NO:834) represents C38 (Dv49-Dv248) concatermer.

The dose response data of F1-F13 are shown in Fig. 5-7.As shown in Figure 5, when feeding with 0.1ppm when raising to the WCR larva, fragment F4-F6 comprises the activity (the dead % of larva) of the sequence (Fig. 4) that derives from Dv248 being significantly higher than contrast.When feeding with 0.02ppm when raising, fragment F6 also shows remarkable activity.The activity of fragment F5 under lowest dose level may be an artefact, because the larva of survival is not showed hypoevolutism.

As shown in Figure 6, when feeding with 0.1ppm when raising the WCR larva, each fragment of F7-F10 has showed that all statistics is active significantly.When feeding with 0.02ppm when raising the WCR larva, fragment F9 and F10 have showed that also statistics is active significantly, and when feeding with 0.01ppm when raising the WCR larva, fragment F10 shows that also statistics is active significantly.0.01ppm the time the F8 activity may be artefact.

As shown in Figure 7, when feeding with 0.02ppm or greater concn when raising the WCR larva, fragment F11-F13 shows that statistics is active significantly.In addition, Zui Da fragment (F12 and F13) shows active in the concentration of 0.005ppm and Geng Gao.

Embodiment 9

Derive from other active concatermer sequence of concatermer C6

As shown in table 9, (carrying out according to for example embodiment 2) identified that the part of the active concatermer C6 (SEQ ID NO:806) that derives from pMON98368 can suppress corn rootworm (WCR) growth and/or survival in food covering biological assay.C6 total length concatermer contains 7 target subfragments, and each length is 70bp, shown in table 6 and table 9.

Table 9 total length C6 concatermer and selected inferior part (SEQ ID NOs:806,847-873) effectiveness in the biological assay of rootworm food

Concatermer	Sequence	Dead % during 1ppm	SEQ ID NO
				The C6 total length	Dv26-Dv49-Dv23-Dv20-Dv13-Dv22- Dv18	100	806
C6.1	Dv26-Dv49	35.3	847
				C6.2	Dv26-Dv49-Dv23	57.1	848
C6.3	Dv26-Dv49-Dv23-Dv20	84.7	849
				C6.4	Dv26-Dv49-Dv23-Dv20-Dv13	51	850
C6.5	Dv26-Dv49-Dv23-Dv20-Dv13-Dv22	41.3	851
				C6.6	Dv49-Dv23	94.6	852
C6.7	Dv49-Dv23-Dv20	75.6	853
				C6.8	Dv49-Dv23-Dv20-Dv13	50	854
C6.9	Dv49-Dv23-Dv20-Dv13-Dv22	56.4	855
				C6.10	Dv49-Dv23-Dv20-Dv13-Dv22-Dv18	52.5	856
C6.11	Dv23-Dv20	74.6	857
				C6.12	Dv23-Dv20-Dv13	63.4	858
C6.13	Dv23-Dv20-Dv13-Dv22	58.5	859
				C6.14	Dv23-Dv20-Dv13-Dv22-Dv18	60.8	860
C6.15	Dv20-Dv13	57.5	861

Concatermer	Sequence	Dead % during 1ppm	SEQ ID NO
				C6.16	Dv20-Dv13-Dv22	20	862
C6.17	Dv20-Dv13-Dv22-Dv18	44.8	863
				C6.18	Dv13-Dv22	71.1	864
C6.19	Dv13-Dv22-Dv18	52.5	865
				C6.20	Dv22-Dv18	44.6	866
C6.28	Dv26	58.9	867
				C6.29	Dv49	72.3	868
C6.30	Dv23	71.9	869
				C6.31	Dv20	62.6	870
C6.32	Dv13	54.2	871
				C6.33	Dv22	56.7	872
C6.34	Dv18	44.6	873

Total length concatermer C7 contains 8 target subfragments, and each subfragment length is 70bp, and is as shown in table 6.Similarly, total length concatermer C12 contains 7 target subfragments from Dv23-Dv13-Dv26-Dv18-Dv49-Dv22-Dv20, and length is 53-80bp, shown in table 6 and table 10.Table 10 total length C12 concatermer and selected subfragment (SEQ ID NOs:811, composition 887-892)

Concatermer	Fragment	The sequence (SEQ ID NO) that comprises with 5 '-3 ' direction
			C12_full length	Dv23-Dv13-Dv26-Dv18-Dv49-Dv22-D v20	811
C12.1	Dv23-Dv13	887
			C12.2	Dv23-Dv13-Dv26	888
C12.3	Dv23-Dv13-Dv26-Dv18	889
			C12.4	Dv23-Dv13-Dv26-Dv18-Dv49	890

Concatermer	Fragment	The sequence (SEQ ID NO) that comprises with 5 '-3 ' direction
			C12.5	Dv23-Dv13-Dv26-Dv18-Dv49-Dv22	891
C12.6	Dv13-Dv26	892
			C12.7	Dv13-Dv26-Dv18	893
C12.8	Dv13-Dv26-Dv18-Dv49	894
			C12.9	Dv13-Dv26-Dv18-Dv49-Dv22	895
C12.10	Dv13-Dv26-Dv18-Dv49-Dv22-Dv20	896
			C12.11	Dv26-Dv18	897
C12.12	Dv26-Dv18-Dv49	898
			C12.13	Dv26-Dv18-Dv49-Dv22	899
C12.14	Dv26-Dv18-Dv49-Dv22-Dv20	900
			C12.15	Dv18-Dv49	901
C12.16	Dv18-Dv49-Dv22	902
			C12.17	Dv18-Dv49-Dv22-Dv20	903
C12.18	Dv49-Dv22	904
			C12.19	Dv49-Dv22-Dv20	905
C12.20	Dv22-Dv20	906

Table 11 has been listed and has been used for fixed other coleopteran pest of target, comprises other sequence of chrysomelid species and other Coccinellidae and Chrysomelidae insect.

Table 11: other Coleoptera target sequence

Sequence and source	SEQ ID NO
		Dbar248_CG6699(D.barberi)	875
Dbal248_CG6699(D.balteata)	876
		Du248_CG6699(D.undecimpunctata howardi)	877
Dz248_CG6699(D.virgifera zea)	878
		Dv248_CG6699(D.virgifera virgifera)	879
Ev248_CG6699(Epilachna varivestis)	880
		Ld248_CG6699(Leptinotarsa decemlineata)	881
Dbal49_CG8055_2(D.balteata)	882
		Db49_CG8055_2(D.barberi)	883
Du49_CG8055_2(D.undecimpunctata howardi)	884
		Dz49_CG8055_2(D.virgifera zea)	885
Dv49_CG8055_2(D.virgifera virgifera)	886

***

According to present disclosure, can prepare and implement open and claimed all compositions and the method for this paper, and not need undo experimentation.Although described the compositions and methods of the invention according to above-mentioned exemplary, those skilled in the art can be clear and definite, under the prerequisite that does not deviate from true concept of the present invention, spirit and scope, can the order of the step of composition described herein, method and step or method be changed, changes, modify and change.More specifically, can be clear and definite, some chemistry reagent relevant with physiology can replace reagent described herein, and can obtain same or analogous result.Those skilled in the art can be clear and definite all these similar replacements and modify all think and be included in spirit of the present invention, scope and the notion that claims limit.

Reference

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Sequence table

<110>BAUM，JAMES A.

CAJACOB，CLAIRE A.

FELDMANN，PESCALE

HECK，GREGORY R.

NOOREN，IRENE

PLAETINCK，GEERT

MADDELEIN，WENDY

VAUGHN，TY T.

＜120〉be used for method and composition at plant Genetic Control insect infestations

<130>MNDE：002US

＜140〉the unknown

<141>2006-09-15

<150>60/718,034

<151>2005-09-16

<160>906

<170>PatentIn Ver.2.1

<210>1

<211>888

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(775)

＜223〉n=a, c, g or t/u

<400>1

cccacgcgtc cggcggctga taaaagccta atgattggag ctgatgtagc agttgcttac 60

attgatggat tcagagggtt tgctacggac tacaatataa cagctttaac accatgtgta 120

aaagtattag gccaatataa aggagtatgc aaggatgaaa tggtaggagg acaggatagc 180

aaccaaatga atacggcagc gagggaaaat ggtattagca taataactta cagaagatcc 240

ttaatttcac ctgatcctgg agataaagag tttccagcag agggttccat atacgccgta 300

tggggggtag gaaaactaga cgaaatgaaa gaacctgctt ttcatgatct ctactcaaag 360

actgatataa aaatggagat gacaccgaaa gaacctcaga ataactgtgt ctcttttacg 420

agatcgaaac ctgaacccaa agaaccttgg aagaaaggtc aaatattcga taaaactata 480

aggatattta agtcttatat tggtccatca ggggccaaaa ggggatacca agccattacc 540

ggccactcat ccacagcttt agcatggtac ataaacggtt tcctcagccc tgagatatgg 600

ctacgacgag gtctttcata tgcatttaaa gtatatggag gaaacaatcc acacagtgct 660

gaatattatc atccattgat aataacagat gaaccccacg gaggttttga tagacttact 720

gacgatgctc agtctaaaat aagagtcttg gctggtgttg aatattctag aaggngaaga 780

cctagaccaa ctacatctgg tgtcctttgt ctatcagaac acaaagaagg aaaagacaga 840

agactcgatg atgacttcga aacgttcaaa aatttcacaa atcttgat 888

<210>2

<211>381

<212>DNA

<213>Diabrotica virgifera

<400>2

aactactcgt ccgcatgggg tacccgcgag gtcactgtcg cagccaaagc gcacgatgca 60

actcaagatg ctacaccaag tgcatcacct gctcctgcgc ccatttcaag ccaaccatta 120

tcattttttg gtaactccaa agataatgcg ggatccctcg agttttcttt cggacgactt 180

tttaaactag tgtgttgtac ctacgatagt agtggcgaag aaacggaagt actcacgatc 240

atacaaacct ccattcagac tatgcatgaa ttcttggccc agatggacaa tcataaacta 300

tgagctacag attcgatcct gaaaccaaga caaatcgcta gtcacactca gcattttgtg 360

ggtttacaag cttgaagatt t 381

<210>3

<211>859

<212>DNA

<213>Diabrotica virgifera

<400>3

tggcgtttta caatgtcaca agtaactgca gattttgaac aatatttgga gagatactta 60

cagggctcct cgaaacttgc aattttgcta gattatgatg gtaccttgac acctatcgtg 120

gctcatccag atctagcagt aattccccca gaagcaaaaa atgttcttga aatgttgtcg 180

caaatcccag aaatatttgt tgctattata tcgggtagag gggtaacgaa tgttaaaaaa 240

ttggttggta ttgactctat tacatatgct ggaaatcatg gactggaagt catctatcca 300

gacggttcca aatacgacca ccaacttcca tctaatttcg aggaccaagt ttaaagaact 360

aatagccaag ttacaagaaa aactgggaag agaaggtgct tggatagaaa acaaaggtgc 420

cagtctaact tttcatttta gagaagcacc agcacactta agggaagaaa tggaaagaga 480

agctaccaaa attatagagg cagcaaattt caaatcttca aaggcacata tggctgtaga 540

ggcaagacca aaagttgatt ggaataaggg aagcgtggcg ctcttgcttt tggagcagaa 600

atacagaaag aactggaaca aagaggccaa ggtgatattt gttggtgatg atacaactga 660

tgaagatgct atgaaggctt tgaaagggaa tgcggctctg tttagaattg ctaagccttg 720

tgctgataca aatgctgata aaattatttc gtctactgaa gatacactaa aaatactgaa 780

aatagctcaa aaacatttta agaaataata tatttcattg atgtatacat atatgtgatt 840

acaataatat atatgtaca 859

<210>4

<211>873

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(745)

＜223〉n=a, c, g or t/u

<400>4

gagagagaca acagataaca gttgtgtagt aaactgttca agtgtaaaca gtaaaccatc 60

agtaaaccag tgacttattt aaacccctga caaacggaac ttattattgt tgctaaaatg 120

gcattttcga tatttagaca atgcgtgaaa aacgggataa cagtttctaa cagaagtgcc 180

cttatggagg gtgttttgaa gaacacgctg gcgaatgtga gttgcagatg tgttcccgtg 240

gcagctgtca attttaacaa ttctatagta gctagaagtt atagcaaaca tgccaaacac 300

acacacgcag aaaggaaata tcagtcagac tcagaatcag atagtgatct ggaatggatt 360

ggacacagag gtgaatcaga tttttggaga agaaaaatca gaaccttcca tggaatcctc 420

gacttaaata aggacggtgt tatttctttt gacgacttca aactgctcag tgatcggttt 480

ataaatctag gtcatttacg ttcagaacaa gtgaaaaaat tccaggatgt tcttaagaat 540

ctttgggaga agcagtttgg agaaatatcc ccttacaact tagtctcagt ggaaagatat 600

ttagaaaata tgcaccacgt gctcaacgat aaaagactgg tcaggaaggc acattccttt 660

ttgccttact tattcaaggc agttgataaa gacagatccg gagaaatctc cgtggaagaa 720

tttaaattat ttttcaatgt attgngcctc aaagaacaag acgccatctt agctttccgg 780

gcaatagact caaacggaaa tgaaaaaatt accccaaaaa gagttcgtga agcacgaaag 840

agatttcttt ttgactgaag accaagaaag gat 873

<210>5

<211>1384

<212>DNA

<213>Diabrotica virgifera

<400>5

ctattgtttc gtaaacttct ttcagttctt tagtaaattt gacagtatct acctcaatag 60

ataccttggc accatgcgag gctttgcctg ctggattcac agcaatacat ttgtagtctc 120

cagagtcaag ttcagaattg gtttctttaa tagttagttc aatgttttct ccatcgaagg 180

tgatctttac cctatcggat ggttccaaag gctcattatt tctaagccag tatacttcag 240

gagtaggatt gccaaccact ttggctttga gggtgagagg agacttttgt ggtacagttt 300

gctcttcaaa tcgtttgacg aacaatggtg gttcctggtt ttctgggaat tcttcaacag 360

ttaggtttgc acttgtttct attgtaccca tagaattggt agctcgaact gtatatgtag 420

cagtgtcatc tgtagatgtc ttgggaatag ttaaagtgta tgtgtttccg tcttttttga 480

attttggtct ggttaaaggt ttaccatcct tcaaccattc gatagtaggt tccgggtttc 540

caaatatagt agtggaaagt gtaactggtt ctcctttcct tacggtaata gattctagag 600

gtgataagac ctcaggtgct tctttctctg aagtaattac tttcagagta aatgttactg 660

aggcttctcc ttccctgttc ctggcgacta tcttaaactt gccttcctga gtcttcttga 720

ttttcttgat ttttagagtg gttttgtaag tatatagctc agaagttgac acaatactga 780

cgttttcagt tacacgtcta ccattacaga accattttat ttcaggttgt ggtactccta 840

ctaatgtact ttcaactacg acgtctgttt cttcaggaac acactgtggt agaggcttct 900

tagcgaatgt tggtggtata ccttctaata tatcagccaa ggaagatgtt cttgatagac 960

tcttccttga aatggaagtc aattcggatc catgagatga caacggacct tctactatga 1020

gttctgttgt actggaggcg aatcctgctg agtttttagc aacgcaagtg aaagttccag 1080

catcttctgg gaatacttcc cttataataa gggtagcgac gttatcttca tcgtagtaga 1140

tttggaaatc tttggaagtt ttgattatgt gagtttgtct gaaccacgta atttggggac 1200

gagggaatcc ttctactttg cattcgaatt ggacagtgta accatctggg gttataatag 1260

gcttcaactt cttgatgaat tttggcgcag aagctttatc tttggatttt ggtttttcaa 1320

atacaggaag tttgtcaatc ttctcgttca atttctgttg tacatactgc gcatactgtt 1380

cttc 1384

<210>6

<211>735

<212>DNA

<213>Diabrotica virgifera

<400>6

ggaaaaggaa gtgggggtga ataggccaaa caatgacaat ggttttagct attaaagatg 60

acgacaacaa gtttgatatg ttggtaagga actgtatggc tcacgatgga aaaagagctc 120

ccatacagtt agtcgaccaa agaggttgtg tcacaagacc aaaattgatg tccagattta 180

ctaagattaa aaactttggc gcaagcgctt cagtgttatc atatgcccac ttccaggcat 240

ttaaattccc tgactccatg gaagttcact tccagtgcac catccaaatc tgcagatatc 300

aatgtccaga tcagtgttca gattcttcag gacaacatct ccacggcttg cttgatttgg 360

tgcatggtcc acctgatact ggttatggtg ctcctccacc gctacctatc gaagcatatt 420

tacatcctgg gcgaccaagg gatgaaagga gatcaaagag agctctcgct gtcgacccgg 480

aaaaggaagt gggggtgaat aggattatta gggttgtatc tacaggagat ttgacattta 540

cacttgacga aaacagcaat agcacaactc cacccactat ggtctttccc gccaaggagg 600

aaattaacaa cggaggatta atatgtatga ctactccagg ttttgcagca accttagtag 660

ttttattagc tatattaata gtatcttgtc ttatgtcagc tttcctttgt gtcaaactaa 720

gaccatttgc tgaaa 735

<210>7

<211>888

<212>DNA

<213>Diabrotica virgifera

<400>7

gaacagcaaa tttttgaagt gtcatactgt ttttatttag caaaatagtt aattgggaac 60

aaagatttta ttagaataat ataccgaaaa tgccgagtca agaattaaca actaataaag 120

taatagtcca ccctttggtt cttctaagtg tggtggacca ttttaatcgc atgggcaaaa 180

tcggcaatca aaagcgtgtt gttggtgttc ttcttggctg ttggcgtgct aaaggagtct 240

tagatgtttc taacagtttt gcagttcctt ttgacgaaga tgacaaggat aagtctgtgt 300

ggttcttgga ccatgattac ttagaaaaca tgtatggaat gttcaaaaaa gttaatgcca 360

gggaacgagt agtaggttgg taccacaccg gccccaaact tcatcaaaat gatattgcca 420

ttaatgagtt aatcagaagg tattgtccaa attccgttct agttatcata gacgctaaac 480

caaaagattt aggtttacca actgaagctt atcaagctgt agaagaagta cacgatgacg 540

gttcacctac atctaaaaca tttgagcatg ttcctagtga aatcggagct gaggaagctg 600

aagaagttgg tgttgaacac ttattgagag atattaaaga cacaactgtt ggtactttgt 660

cacagagaat aacgaatcag cttttagggt taaaaggtct ccattcacag ttacgtgata 720

ttagggacta tttggtacaa gtgtgtggta accagcttcc aatcaatcac caaatcatct 780

accaattaca agacatattt aatcttttgc ccgatataaa tcaggatgca tttaatacat 840

cgttttacgt gaaaaataac gatcaaatgt tagtcgttta tctagcag 888

<210>8

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>8

ccgaaaatgc cgagtcaaga attaacaac 29

<210>9

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>9

gcatcctgat ttatatcggg caaaag 26

<210>10

<211>746

<212>DNA

<213>Diabrotica virgifera

<400>10

ccgaaaatgc cgagtcaaga attaacaact aataaagtaa tagtccaccc tttggttctt 60

ctaagtgtgg tggaccattt taatcgcatg ggcaaaatcg gcaatcaaaa gcgtgttgtt 120

ggtgttcttc ttggctgttg gcgtgctaaa ggagtcttag atgtttctaa cagttttgca 180

gttccttttg acgaagatga caaggataag tctgtgtggt tcttggacca tgattactta 240

gaaaacatgt atggaatgtt caaaaaagtt aatgccaggg aacgagtagt aggttggtac 300

cacaccggcc ccaaacttca tcaaaatgat attgccatta atgagttaat cagaaggtat 360

tgtccaaatt ccgttctagt tatcatagac gctaaaccaa aagatttagg tttaccaact 420

gaagcttatc aagctgtaga agaagtacac gatgacggtt cacctacatc taaaacattt 480

gagcatgttc ctagtgaaat cggagctgag gaagctgaag aagttggtgt tgaacactta 540

ttgagagata ttaaagacac aactgttggt actttgtcac agagaataac gaatcagctt 600

ttagggttaa aaggtctcca ttcacagtta cgtgatatta gggactattt ggtacaagtg 660

tgtggtaacc agcttccaat caatcaccaa atcatctacc aattacaaga catatttaat 720

cttttgcccg atataaatca ggatgc 746

<210>11

<211>1123

<212>DNA

<213>Diabrotica virgifera

<400>11

aacaataaat tttattgcga tgatttctca caagaaaaaa taatttttta tatacatata 60

aacataaata tgaatatatt ttgagaaaaa aataaggtag tataactgaa acctaataat 120

tttctaagga ttttaaaaaa tatctgcttg acaaggatac aattagggta acaacaaact 180

taactgttta aaatacttca aaaaaaggaa tggtttatta tatatatcgt atatacatag 240

tagtttaaaa ggtttttgaa gatctgtcta agcaaccaac aatgttttac aatactatat 300

aaaatacagg caatacacag tatccaaaaa atacttaatc agtgaatttt ctgttgacgt 360

tgcgtccgaa cagagcatta cggatctggg gaatcaattg ttgtgagatg agctcaagac 420

gggcttccag agtattgttg attttgatct tgtttctcaa ggccaacagt tcgattcctc 480

cggtggtttc ttgagaaagg tggctctcgt cgtcgatttt tagatttacg tctttaccgg 540

ttatgtcctt gtacttttgg gagacgttag gcatgataga ttttaccaat tctctgtcct 600

gagggcgtac tctaatggtg atgtcctttt cgaagagctg atagagccct tggaggatga 660

gactttccag gatttgtgta tatttgcctg aatctctggt tacctcacca agacgtttgc 720

gagcatcttc caaaacggca cgtacatggt cttcccttac tttcaatacc ttcaatcttg 780

cctggttcaa catgtttgat gattggattt ttttctggag ttctacttgc ttctcttttt 840

tctcgtagta ctccataatc ttgagcctct gttgttggac cagacggccc ttttcgatgt 900

tgaattcttc ttcagccttt gcatcaattt cctcggcctt ttcattggct tcttgctcaa 960

tgaaagccat catgtgcttg atctgctttt gtacatctgc gtcgctgaga gccattttga 1020

gcctttttct ttcgacttag aggaaattaa agggatttaa acaatgtttt tcccgactac 1080

gcccagcaac tttctgaaca atttgctcaa taaaaatcgg acg 1123

<210>12

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>12

gacgttgcgt ccgaacagag cattac 26

<210>13

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>13

gttcagaaag ttgctgggcg tagtcg 26

<210>14

<211>743

<212>DNA

<213>Diabrotica virgifera

<400>14

gacgttgcgt ccgacagagc attacggatc tggggaatca attgttgtga gatgagctca 60

agacgggctt ccagagtatt gttgattttg atcttgtttc tcaaggccaa cagttcgatt 120

cctccggtgg tttcttgaga aaggtggctc tcgtcgtcga tttttagatt tacgtcttta 180

ccggttatgt ccttgtactt ttgggagacg ttaggcatga tagattttac caattctctg 240

tcctgagggc gtactctaat ggtgatgtcc ttttcgaaga gctgatagag cccttggagg 300

atgagacttt ccaggatttg tgtatatttg cctgaatctc tggttacctc accaagacgt 360

ttgcgagcat cttccaaaac ggcacgtaca tggtcttccc ttactttcaa taccttcaat 420

cttgcctggt tcaacatgtt tgatgattgg atttttttct ggagttctac ttgcttctct 480

tttttctcgt agtactccat aatcttgagc ctctgttgtt ggaccagacg gcccttttcg 540

atgttgaatt cttcttcagc ctttgcatca atttcctcgg ccttttcatt ggcttcttgc 600

tcaatgaaag ccatcatgtg cttgatctgc ttttgtacat ctgcgtcgct gagagccatt 660

ttgagccttt ttctttcgac ttagaggaaa ttaaagggat ttaaacaatg tttttcccga 720

ctacgcccag caactttctg aac 743

<210>15

<211>2325

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(107)..(185)

＜223〉n=a, c, g or t/u

<400>15

atcgtcatcg tccaaatcca tagcaacatt gggattcaaa gccctctctc tttctctcct 60

aatttcggtt tcgatgctct gcctgatggc cagtttgcaa gctctcntgg cagatttcag 120

tcaaatcnng gcacccagaa aatcccatga gttactttag caatgtatac aagatctacg 180

tcttntagct acaggcgatt tcctcaagtt ggctttgaag atggcttccc tagatttctc 240

gtcgggtaga gggatgtaga tcaactgatc gagacgacct ggacgcaata ttgcaggatc 300

gatgatatct ggtctgtttg tagcaccgat aataaacacg ttctttttgg ctcccatacc 360

gtccatttct gttagaattt gattaataac tctgtcggct gcaccaccgg catcacctac 420

atttccacca cgcgatttgg caattgaatc taattcgtca aagaatagaa cacaaggtgc 480

cgcagatcga gccttgtcga agatatctct gacatttgct tctgattcac caaaccacat 540

agtaagcaac tctggtcctt taactgagat aaagttggct tggcattcat tcgcaatagc 600

cttagctaat aacgttttac cgcaaccagg cggcccatag aacaacactc ctctggacgg 660

ttgcataccg aacttaagga acttatctgg atgttcgact gggtattgca ccaattcttg 720

caattctttc ttgacattag ccaagccacc gatatcgtcc caagtgacgt ttggtacttc 780

tactatggtc tcacgtaaag cacttgggct gctcttcgtc atggcgtaac ggaagttctc 840

catggtaact gccagtgagt ttaggacttc tgcgtcaatt tggtcgtctt cgaggtcgat 900

gaggtccatt ttttctctga tttgttgaag ggctgcttcc gaacacaatg aagctaaatc 960

agcacctacg tgaccatgag tttccgcagc gatctgttcc aagtcgacat catcggccaa 1020

tttcatgttc ttggtgtgaa tacgtaaaac ttcaagtctg ccagttgcat caggtattcc 1080

gatatcgatt tccctgtcga atcttccgaa tctacgtaaa gctggatcga ttgagtttgg 1140

tctgttagta gcggccataa cgataacgtg ggaactcttc ttcataccgt ccatcaaagt 1200

tagaagttgg gatactatac ggcgttccac ctctccgtgt gttttctctc tcttgggagc 1260

gatggcatcc aattcatcaa tgaaaataat agctggggaa ttcttgtcgg cttcctcgaa 1320

agctttacga agattgcttt cggattcacc ggccaatttg ctcataatct caggaccgtt 1380

aatcaagaag aagaacgctc cagtttcgtt agctacggca cgtgcaatga gtgtcttacc 1440

agtacccggt ggaccgtaaa gtaagatacc tcttggtggt ttaactccaa tagccttgaa 1500

aagactagga tgtctcagtg gtaactccac catttctttg atttgagcga gctgttttct 1560

acaacctccg atgtcgtcgt atccaactgc gttaagagct tcttcctcct cttcgcgctt 1620

gattggatct ccatcacaat gaatgacggt atctggagcc actatacagt acggagcggg 1680

atcggtctcg acgactttga attcaacagc tctcattcct ccacgtacaa cgaatacgtc 1740

atctttgtgt attggtcgat aggcctccag gaagtagggt ttcaggtata cttcgaagag 1800

atttcctgtg agtccttcca ctgtatcatc tatgggaagt acatgtattc tcttgccata 1860

ttttacatca gggcaagctt ggatagaaac tacatctgaa agcctaactc ttaaattatt 1920

tctaacattt ctattcatac gaattttttc atcgctacat gtatcatcag ataatactat 1980

acaaacagtt tctttccttc gcttgccctt cagtaggact gtgtctcctc tgaaaagttg 2040

tagttcatcc atttttgctt gtgacaaagc aaccacggag ttatcatcgt taactgcttc 2100

ttctaccaaa agtctatttg gacgatcttt cttacggaga attgcggtgg ccaaatcctc 2160

tggatttttg ttttcggcca ttatttagta atgaatatgt acttaatgta gaaacttttg 2220

aaaaatcagt tcacaataga aattatctct tttatatgat ttcagttttt actcaagcac 2280

aaagatgacg atcgttattc tgacacaaaa tgacggacgc gtggg 2325

<210>16

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>16

tcgtcatcgt ccaaatccat agcaac 26

<210>17

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>17

ggaaactcat ggtcacgtag gtgctg 26

<210>18

<211>978

<212>DNA

<213>Diabrotica virgifera

<400>18

tcgtcatcgt ccaaatccat agcaacattg ggattcaaag ccctctctct ttctctccta 60

atttcggttt cgatgctctg cctgatggcc agtttgcaag ctctctggca gatttcagtc 120

aaatcggcac cagaaaatcc atgagttact ttagcaatgt atacaagatc tacgtcttta 180

gctacaggcg atttcctcaa gttggctttg aagatggctt ccctagattt ctcgtcgggt 240

agagggatgt agatcaactg atcgagacga cctggacgca atattgcagg atcgatgata 300

tctggtctgt ttgtagcacc gataataaac acgttctttt tggctcccat accgtccatt 360

tctgttagaa tttgattaat aactctgtcg gctgcaccac cggcatcacc tacatttcca 420

ccacgcgatt tggcaattga atctaattcg tcaaagaata gaacacaagg tgccgcagat 480

cgagtcttgt cgaagatatc tctgacattt gcttctgatt caccaaacca catagtaagc 540

aactctggtc ctttaactga gataaagttg gcttggcatt cattcgcaat agccttagct 600

aataacgttt taccgcaacc aggcggccca tagaacaaca ctcctctgga cggttgcata 660

ccgaacttaa ggaacttatc tggatgttcg actgggtatt gcaccaattc ttgcaattct 720

ttcttgacat tagccaagcc accgatatcg tcccaagtga catttggtac ttctactatg 780

gtctcacgta aagcacttgg gctgctcttc gtcatggcgt aacggaagtt ctccatggta 840

actgccagtg agtttaggac ttctgcgtca atttggtcgt cttcgaggtc gatgaggtcc 900

attttttctc tgatttgttg aagggctgct tccgaacaca gtgaagctaa atcagcacct 960

acgtgaccat gagtttcc 978

<210>19

<211>637

<212>DNA

<213>Diabrotica virgifera

<400>19

cccacgcgtc cgcccacgcg tccgcggact aaaatgaagc aaattgtaac aaaccaaacg 60

gtaaaaattc ctgagggaat tacagtaacc actaagtcaa gggtagtaac tgtaaaagga 120

ccccgaggta atctgaagag atccttcaaa catcttaccc tggacattcg aatgatcaac 180

cccaggttac tgaaagtaga gaaatggttc ggtaccaaga aggaattagc tgctgtcaga 240

acagtatgct ctcatgttga aaacatgtta aagggtgtta caaaaggata ccaatacaag 300

atgagagcag catatgctca ctttcccatt aactgtgtca ccactgaagg aaacacagtt 360

atcgaaatta gaaatttctt gggagaaaaa tacatcagaa gagtaaagat ggccccaggt 420

gttacagtag taaactccac caaacaaaag gatgaactta tcattgaagg aaactcattg 480

gaggatgttt caaagtcagc tgcccttatt caacaatcta caacagttaa aaataaggat 540

atccgtaaat tcttagatgg gctttatgta tctgaaaaaa caactgttgt acaagaagaa 600

taacttgtat ttttgaaata aatttaagtt tatattt 637

<210>20

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>20

ggaattacag taaccactaa gtcaagggta g 31

<210>21

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>21

cagatacata aagcccatct aagaatttac g 31

<210>22

<211>499

<212>DNA

<213>Diabrotica virgifera

<400>22

ggaattacag taaccactaa gtcaagggta gtaactgtaa aaggaccccg aggtaatctg 60

aagagatcct tcaaacatct taccctggac attcgaatga tcaaccccag gttactgaaa 120

gtagagaaat ggttcggtac caagaaggaa ttagctgctg tcagaacagt atgctctcat 180

gttgaaaaca tgttaaaggg tgttacaaaa ggataccaat acaagatgag agcagcatat 240

gctcactttc ccattaactg tgtcaccact gaaggaaaca cagttatcga aattagaaat 300

ttcttgggag aaaaatacat cagaagagta aagatggccc caggtgttac agtagtaaac 360

tccaccaaac aaaaggatga acttatcatt gaaggaaact cattggagga tgtttcaaag 420

tcagctgccc ttattcaaca atctacaaca gttaaaaata aggatatccg taaattctta 480

gatgggcttt atgtatctg 499

<210>23

<211>678

<212>DNA

<213>Diabrotica virgifera

<400>23

aaattctttt acggaagaca cgttgagagg tgcatttgca ggatgagttc cttaaaactt 60

cagaagaggc tagcagcctc tgttatgcga tgtggtaaaa agaaagtatg gttggaccct 120

aatgaaatca acgaaattgc caacactaac tcaagacaga acatccgtaa gttgataaag 180

gatggtctta ttattaagaa gcccgtagct gtacattccc gtgcccgtgt tcgcaaaaac 240

actgaagccc gcaggaaagg aaggcactgc ggttttggta aaaggaaggg tactgctaat 300

gcccgtaccc cacaaaagga attatggatt caacgcatga gagttttgcg tcgtctcctt 360

aaaaaataca gggaagctaa aaaaattgac agacatctat accactcact ctacatgaag 420

gccaagggta acgtattcaa gaacaagcgt gtccttatgg aatacatcca caagaagaag 480

gcagagaaag cccgtgccaa gatgttggca gaccaggcca atgccagaag gatgaaggta 540

aaacaggcta gagaaagacg tgaggaacgt atcgccacaa agaaacaaga agttttgcag 600

aactacatga gggaggatga agctgcggcc actaagaaat aagttaattg ttttataaga 660

tgactatatt aaaaattt 678

<210>24

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>24

ctagcagcct ctgttatgcg atgtgg 26

<210>25

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>25

gcttcatcct ccctcatgta gttctgc 27

<210>26

<211>554

<212>DNA

<213>Diabrotica virgifera

<400>26

ctagcagcct ctgttatgcg atgtggtaaa aagaaagtat ggttggaccc taatgaaatc 60

aacgaaattg ccaacactaa ctcaagacag aacatccgta agttgataaa ggatggtctt 120

attattaaga agcccgtagc tgtacattcc cgtgcccgtg ttcgcaaaaa cactgaagcc 180

cgcaggaaag gaaggcactg cggttttggt aaaaggaagg gtactgctaa tgcccgtacc 240

ccacaaaagg aattatggat tcaacgcatg agagttttgc gtcgtctcct taaaaaatac 300

agggaagcta aaaaaattga cagacatcta taccactcac tctacatgaa ggccaagggt 360

aacgtattca agaacaagcg tgtccttatg gaatacatcc acaagaagaa ggcagagaaa 420

gcccgtgcca agatgttggc agaccaggcc aatgccagaa ggatgaaggt aaaacaggct 480

agagaaagac gtgaggaacg tatcgccaca aagaaacaag aagttttgca gaactacatg 540

agggaggatg aagc 554

<210>27

<211>927

<212>DNA

<213>Diabrotica virgifera

<400>27

ctttacgcgt ccgatatttt tcatagattt agttttaaat actgacaatt aagatgccgg 60

accatttagg agatgatatg cgaaaaataa aagaacagaa agaggagccc gaaaaggaaa 120

ttaaagccct ggatgaaggt gatatagcac ttcttaaaac ctatggacaa gggcaataca 180

caaagacaat taaaacatgt gaagaagata tccaaacgat aatcaaaaga gtaaatgaat 240

taactggtat aaaagaatct gatactggtt tagctccacc agctttatgg gatttggcag 300

ctgacaaaca aaccttacaa aatgagcaac ccttacaagt ggcccgttgt acgaaaatta 360

tcaacgctga tacagatgat cccaaataca ttataaacgt taaacagttt gccaaattcg 420

tagtagattt ggcagactct gtggccccta cagacataga agaaggcatg agagttggag 480

tcgaccgaaa taagtatcaa attcatattc ctctacctcc aaaaattgat cccactgtga 540

ctatgatgca agttgaggag aagcctgatg ttacatacag tgatgttggt ggctgcaagg 600

aacaaattga aaagttgaga gaagttgttg aaacaccttt actccatcct gaaaaatttg 660

taaaacttgg tattgaacca ccaaaaggcg ttctcctttt tggcccccct ggaactggta 720

aaactctttg tgctagagca gtagccaata gaacagatgc ctgctttatt cgtgtaattg 780

gttctgaact ggttcagaag tacgtaggag agggagcaag aatggttaga gaacttttcg 840

aaatggctag gtctaagaag gcctgcttga tattctttga ttgaattgat gccattggct 900

ggagccagat ttgatgatgg cgctgga 927

<210>28

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>28

gaacagaaag aggagcccga aaagg 25

<210>29

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>29

gcatcaattc aatcaaagaa tatcaagcag g 31

<210>30

<211>800

<212>DNA

<213>Diabrotica virgifera

<400>30

gaacagaaag aggagcccga aaaggaaatt aaagccctgg atgaaggtga tatagcactt 60

cttaaaacct atggacaagg gcaatacaca aagacaatta aaacatgtga agaagatatc 120

caaacgataa tcaaaagagt aaatgaatta actggtataa aagaatctga tactggttta 180

gctccaccag ctttatggga tttggcagct gacaaacaaa ccttacaaaa tgagcaaccc 240

ttacaagtgg cccgttgtac gaaaattatc aacgctgata cagatgatcc caaatacatt 300

ataaacgtta aacagtttgc caaattcgta gtagatttgg cagactctgt ggcccctaca 360

gacatagaag aaggcatgag agttggagtc gaccgaaata agtatcaaat tcatattcct 420

ctacctccaa aaattgatcc cactgtgact atgatgcaag ttgaggagaa gcctgatgtt 480

acatacagtg atgttggtgg ctgcaaggaa caaattgaaa agttgagaga agttgttgaa 540

acacctttac tccatcctga aaaatttgta aaacttggta ttgaaccacc aaaaggcgtt 600

ctcctttttg gcccccctgg aactggtaaa actctttgtg ctagagcagt agccaataga 660

acagatgcct gctttattcg tgtaattggt tctgaactgg ttcagaagta cgtaggagag 720

ggagcaagaa tggttagaga acttttcgaa atggctaggt ctaagaaggc ctgcttgata 780

ttctttgatt gaattgatgc 800

<210>31

<211>831

<212>DNA

<213>Diabrotica virgifera

<400>31

attgaaaata atgtaatcaa ccattacatt taatgtgttt tggctgctaa cctcttgtct 60

ctctcttcag caatagacaa tttgactccc tttcctcttg ggagggaaac gaaggcctta 120

gctcccttac caataatgaa aacgttattt aacctagtag caaatacatg accattagca 180

tctttaacat gcacaatatc aaaggatcca ggatgacgtt ccctattgat aactgtacct 240

acacgaccca aatttcttcc tccagtgatc atacataagt ttccagattc gaacttaatg 300

tagtctagaa ttttagatgt ggcaatttcc aattggatgg tgtcattgac tttgatgttt 360

gggtcagggt atctgatggt acggccatca tgtgtcacca agaatggaat tccttttggt 420

ccagtttgga ctcttcgtac tttgcagagt ttgtattttg cttcttcagc ggtaatacgg 480

tgaatggcaa atctaccttt aacatcatag ataagacgga agaattcacc agtcttctca 540

atagtaataa catccataaa tcctgcagga tagttactgt ctgttctgac ttttccatcg 600

actttaatta atctttgcat aacaattttt gtgacttcac tgttggtgag ggcatacttt 660

agtctgtttc ttaagaaaat tactaatggt aaagattctc tcaacttgtg aggaccggtg 720

gatggacggg gggcaaatac acctcccaat ttgtccaaca tccatgcttt gggtgcgttt 780

aaacgcttca aatgcttttt aggtcctcga gccatcttga ctttaaaaat a 831

<210>32

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>32

gtgttttggc tgctaacctc ttgtctctc 29

<210>33

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>33

gcatggatgt tggacaaatt gggag 25

<210>34

<211>733

<212>DNA

<213>Diabrotica virgifera

<400>34

gtgttttggc tgctaacctc ttgtctctct cttcagcaat agacaatttg actccctttc 60

ctcttgggag ggaaacgaag gccttagctc ccttaccaat aatgaaaacg ttatttaacc 120

tagtagcaaa tacatgacca ttagcatctt taacatgcac aatatcaaag gatccaggat 180

gacgttccct attgataact gtacctacac gacccaaatt tcttcctcca gtgatcatac 240

ataagtttcc agattcgaac ttaatgtagt ctagaatttt agatgtggca atttccaatt 300

ggatggtgtc attgactttg atgtttgggt cagggtatct gatggtacgg ccatcatgtg 360

tcaccaagaa tggaattcct tttggtccag tttggactct tcgtactttg cagagtttgt 420

attttgcttc ttcagcggta atacggtgaa tggcaaatct acctttaaca tcatagataa 480

gacggaagaa ttcaccagtc ttctcaatag taataacatc cataaatcct gcaggatagt 540

tactgtctgt tctgactttt ccatcgactt taattaatct ttgcataaca atttttgtga 600

cttcactgtt ggtgagggca tactttagtc tgtttcttaa gaaaattact aatggtaaag 660

attctctcaa cttgtgagga ccggtggatg gacggggggc aaatacacct cccaatttgt 720

ccaacatcca tgc 733

<210>35

<211>3173

<212>DNA

<213>Diabrotica virgifera

<400>35

gtcaaccacc tcaacaaagt taaggctaag ttggaacaaa ccttggacga actcgaagac 60

tctttggaac gcgaaaagaa actccgtggt gacgtagaaa aatctaaacg taaggttgaa 120

ggcgacttga aactcaccca agaggctgta gctgacttgg aacgcaacaa gaaggaactc 180

gaacaaacca tccaacgcaa agacaaggaa atctcatcac tcactgccaa actcgaagac 240

gaacaatccg ttgttggtaa acaacaaaaa caagttaagg aactccaggc tcgcattgag 300

gaactcgaag aggaagttga agctgaacgt caagcccgtg ctaaagctga gaaacaacgc 360

gctgatttgg cccgcgaatt ggaagaactc ggcgaacgtc ttgaagaagc cggtggtgct 420

acctcagccc aaatcgaact caacaagaaa cgtgaagccg aactcgccaa actccgtcgt 480

gacctcgaag aatccaacat ccaacatgaa ggaactcttg ctaacctccg caagaagcac 540

aacgacgctg tttctgaaat gggtgaacaa attgaccaac tcaacaaact caaagccaag 600

gctgagaaag aaaagtctca atacttcggt gaactcaatg acctccgtgc ttcagtcgac 660

cacttagcca acgaaaaggc cgctatagaa aaggtatcca aacaattggg acaacaactc 720

aacgacgtcc aaggcaaact tgacgaaacc aaccgcactc ttaacgactt cgatgccgcc 780

aagaagaagt tgtctattga aaactccgac ctcctcagac aactcgagga agccgaatct 840

caagtatctc aactcagcaa gatcaaggta tcccttacca ctcaattgga agataccaag 900

agattggccg atgaagagag cagagaacgc gctaccctcc tcggcaaatt cagaaacttg 960

gaacacgact tggacaacat ccgtgaacaa gttgaagaag aagccgaagc caaggctgat 1020

atccaaagac aactcagcaa agccaacgct gaatctcaac tctggcgcca gaaatacgaa 1080

tctgaaggag ttgctcgctc tgaagaactc gaagaagcta agaggaaact ccaagctcgt 1140

cttgctgagg ctgaagaaac catcgaatct ctcaaccaga aggttgtagc tcttgaaaag 1200

accaaacaac gtttggccac tgaagtcgaa gacttgcaac tcgaagtcga ccgtgctaac 1260

gccattgcca atgctgctga gaagaaacaa aaggccttcg acaaaatcat tggagaatgg 1320

aaactcaagg ttgatgactt ggctgctgaa ctcgatgcca gtcagaaaga atgcagaaac 1380

tactccaccg aattgttcag acttaagggc gcctacgaag aaggacaaga acaacttgaa 1440

gctgtccgcc gtgaaaacaa gaacctcgct gatgaagtta aggatctcct tgaccaaatt 1500

ggagaaggtg gccgcaacat ccacgaaatc gaaaaggccc gcaagcgctt ggaagctgaa 1560

aaagatgaac tccaagctgc tcttgaagaa gctgaagctg ctcttgaaca agaagaaaac 1620

aaagtacttc gtagccaact tgaactttct caagtccgtc aagaaatcga cagacgtatc 1680

caagagaaag aagaagaatt cgaaaacacc cgcaagaatc accaacgtgc cctcgactcc 1740

atgcaagcct cccttgaagc tgaagccaaa ggtaaggctg aggcccttcg catgaagaag 1800

aagttggaag ctgatatcaa tgaattggaa atcgctttgg accacgctaa caaggccaac 1860

gctgaagccc aaaagaccat caaacgctac caacaacaac ttaaggacac ccaaaccgcc 1920

cttgaagaag aacaaagagc tcgcgatgaa gcccgtgaac aacttggaat ttcagaacgt 1980

cgtgctaacg ctcttcaaaa tgaacttgaa gaatctcgca ctcttctcga acaagccgac 2040

cgtgctcgtc gccaagctga acaagaattg ggagatgctc acgaacaatt gaacgatctt 2100

ggagcccaaa acgcttcctt gtctgccgcc aagaggaaat tggaaaccga attgcaaacc 2160

ctccactcag accttgacga acttctcaac gaagccaaga actccgaaga gaaggccaag 2220

aaggctatgg tagatgccgc tcgtctcgct gatgaactcc gcgctgaaca agaccatgcc 2280

caaacacaag agaaactccg taaagctctc gaagctcaaa tcaaggacct ccaagtacgc 2340

ttggacgaag ctgaagccaa tgcccttaaa ggaggaaaga aggctattgc caaattggaa 2400

caacgtgtta gagaattaga aaatgaatta gacggtgaac aaaggcggca cgcggatgcc 2460

caaaagaact tgagaaagtc tgaacgtcgt atcaaggaac ttagcttcca agctgaagaa 2520

gaccgcaaga accacgagag gatgcaagac ctcgtcgaca aactccaaca aaagatcaag 2580

acctacaaga gacagataga agaagccgaa gaaatcgccg ctttgaactt ggccaaattc 2640

cgtaaagccc aacaagagct cgaagaagcc gaagaaagag ccgacctcgc cgaacaggcc 2700

atcgccaaat tccgtgccaa gggacgttca ggatctgcag ctagaggagc cagtccagcg 2760

ccccaaagac agcgccccac cttgggaatg ggagacgctc taggtggtcc attcccccca 2820

aggttcgact tagcacctga tttcgagtga acatgcgcgc tgtgttagaa tcttaataaa 2880

caaacgaact gtttaggtcg catcaaaacc aactctatgc gattttactt ttattaattt 2940

tttattttcg tttttgaata ttagagttgt atgattccgt aaatgttcga gaaaaaggag 3000

aacgaaggga gtattcagag acacgtattt accaccaaga cagccctttg atctcttcat 3060

ttctcaaaat gccaattatt ttgttatgaa aatatgtagg ttgtataatg atattgtatg 3120

tatgataatg ttgtatgata taaggcacca ataaacataa aagttatatt gtt 3173

<210>36

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>36

ggtttggatg ctgaattata ccgtctaggt c 31

<210>37

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>37

ggtgatcctt ggtggctttg tcttg 25

<210>38

<211>672

<212>DNA

<213>Diabrotica virgifera

<400>38

ggtttggatg ctgaattata cgtctaggtc acactaaggt cttcttccgt gctggtgtct 60

tgggtcagat ggaagaactt cgtgacgacc gtcttggcaa gatcgtcacc tggatgcaat 120

cttgggtccg tggttatctc tcaagaaagg aattcaagaa actgcaagaa caacgtttgg 180

ccctccaagt gtgccaacgc aacttgcgca aatacctcaa attgcgcaca tggccatggt 240

acaaattgtg gcagaaggtc agacctctcc tcaacgtcac ccgtatcgag gatgagattg 300

ccaaacttga agagaaagcc caaaaagccc aagaagcctt cgagcgtgaa gaaaaggcca 360

agaaggaatt agaagctctc tatgccaagc tcttagctga gaagaccgag ttgttatcta 420

acttggaagg agaaaaggga tccctttccg aaactcaaga aagggccaac aaactccaag 480

cccaaaagaa cgacctcgag tctcaacttt ctgaaaccca agatcgtttg agccaagagg 540

aagatgctcg caaccagttg atgcaacaaa agaagaaact cgaacaagaa atgtccggtt 600

tcaagaaaga catcgaagat ttggaactca acctccaaaa gtctgaacaa gacaaagcca 660

ccaaggatca cc 672

<210>39

<211>1222

<212>DNA

<213>Diabrotica virgifera

<400>39

tgagctgttg aactcagtga ctagaatcaa aaagtagttg gagcaagcgt tctatcgcac 60

cctttcaacc gaacggcttg gtcgagtact tttctgtacc tacgaaccca gaagaaaatt 120

cagtttgtgt gaaaagttca ataagttaca agcattatag tttaaagcgt taggttgatt 180

aataatattt aagtatattt ttcttcctta tctatacaca cacgacatta agattaaagt 240

ataaagaaca actatatcgt atctttttct ttttgttttt tataaaatat ttagtaacag 300

gaaagcacaa aacatgcata tgagctcttt agatttatac agtgaaatgg acttaaagct 360

ataaactgcg ctaccaactc gaccacctga tttgctatgt ggtgatttta aaagccgtga 420

agtcgataca taggagtaaa tatagagaag ccgcctctac aaacaaaaca ccgcctccca 480

aacggacaga cgtctcacag actcctgtcg atttggacgg cgatgtttca gcgacaggaa 540

acaaaaatgt cttgctgttc taagattttt ttgtaaatcg cgtatttcta aaagaccagg 600

tgtcgagttg aagtagggtg gtggtgacgc ctagacgtca acgaccattt tcttgtggat 660

atcggtgttg ccgaccacgt tgcaaaattc ctcgaaggaa attttgccgt cttcatcttt 720

gtcggcgaac aggatggtct tgtcgacgat ctgctggagc tgagtgtcct tcagattgtt 780

tccgaccatc attttgagaa cctgaaatag ttctccatta gagatgtatc cgtcattatc 840

catatcgtag atccggaaag caaatctaag cttggagagc ttgtcaccct ttacgctgaa 900

ttgagacaca ccctgaatga attctttgaa atcgacttct ccgttaccat cggcatcgaa 960

gatgtcaatc acacgttgaa cgagcggatt ctgctgcagc tccggcagag acatgaactc 1020

gtcgatgctg agggcccccg agttgtccag atctaatttt ctgaatcttt taccgagacg 1080

tcgaatttcg tcggcgtcaa agttagagca tagctccatg ggcagagagc tctcgttacc 1140

catggtctgc tccaagtact ctaacttcta attgatttat gattcttttt aatgatcgac 1200

aaactgagga taaataattt ac 1222

<210>40

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>40

cgtctcacag actcctgtcg atttgg 26

<210>41

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>41

caattagaag ttagagtact tggagcagac catg 34

<210>42

<211>684

<212>DNA

<213>Diabrotica virgifera

<400>42

cgtctcacag actcctgtcg atttggacgg cgatgtttca gcgacaggaa acaaaaatgt 60

cttgctgttc taagattttt ttgtaaatcg cgtatttcta aaagaccagg tgtcgagttg 120

aagtagggtg gtggtgacgc ctagacgtca acgaccattt tcttgtggat atcggtgttg 180

ccgaccacgt tgcaaaattc ctcgaaggaa attttgccgt cttcatcttt gtcggcgaac 240

aggatggtct tgtcgacgat ctgctggagc tgagtgtcct tcagattgtt tccgaccatc 300

attttgagaa cctgaaatag ttctccatta gagatgtatc cgtcattatc catatcgtag 360

atccggaaag caaatctaag cttggagagc ttgtcaccct ttacgctgaa ttgagacaca 420

ccctgaatga attctttgaa atcgacttct ccgttaccat cggcatcgaa gatgtcaatc 480

acacgttgaa cgagcggatt ctgctgcagc tccggcagag acatgaactc gtcgatgctg 540

agggcccccg agttgtccag atctaatttt ctgaatcttt taccgagacg tcgaatttcg 600

tcggcgtcaa agttagagca tagctccatg ggcagagagc tctcgttacc catggtctgc 660

tccaagtact ctaacttcta attg 684

<210>43

<211>864

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(842)

＜223〉n=a, c, g or t/u

<400>43

gtagcaagga ctattgccag atcattttgt ccagagctgt atggatcttc agctcttgaa 60

acaacagaag tagataactg gttaacgctt tcccttggtc cacttaataa tagaagcgaa 120

gttaccggtg tcttagaaac cctaaataaa gttttaggtt cagttactta cttggttgct 180

aaaagaatta ctgttgctga tttcattgtt tttggtagct tatatacaaa caaacactgg 240

aatgatttaa ccaaatctaa atcggcacca gttaatgttg ttcgttggta cgactttcta 300

catgcacaag aagctgttat gaacatttta aataacctac cccaagatgt agcagcttcg 360

ttgacacaag cagaaaaaca agttagtcaa gagaaaaata ctaatattgg taatcgaaat 420

caagagggaa aatttgtcga tttgcctttt gcacagatgg gtaaagttat tgtcaggttt 480

ccaccagaag catcaggtta tttgcacatc ggccacgcca aagctgccct tctcaaccaa 540

cactatcagg aagctttcca ggggaaactt attttacgtt tcgatgatac taatcctgcc 600

aaagaaaatg tgcattttga aaacgttatt ctggaagatg tagcaatgtt acagattaaa 660

cccgacatat tcacatatac ctcagattac tttgaactta tgttagagtt atgtgaaaaa 720

atgatgaaag aaggaaatgc ctatgtagat gatactgaac cagaggaaat gaaaactcaa 780

agagaacaaa aaatcgagtc agtcaacagg aataatgatg ttcagaagaa tttgctatgt 840

gngaagaatg aaaaaggtac cgag 864

<210>44

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>44

caaggactat tgccagatca ttttgtcc 28

<210>45

<211>33

<212>DNA

<213>Diabrotica virgifera

<400>45

gattttttgt tctctttgag ttttcatttc ctc 33

<210>46

<211>790

<212>DNA

<213>Diabrotica virgifera

<400>46

caaggactat tgccagatct tttgtccaga gctgtatgga tcttcagctc ttgaaacaac 60

agaagtagat aactggttaa cgctttccct tggtctactt aataatagaa gcgaagttac 120

cggtgtctta gaaaccctaa ataaagtttt aggttcagtt acttacttgg ttgctaaaag 180

aattactgtt gctgatttcg ttgtttttgg tagcttatat acaaacaaac actggaatga 240

tttaaccaaa tctaaatcgg caccagttaa tgttgttcgt tggtacgact ttctacatgc 300

acaagaagct gttatgaaca ttttaaataa cctaccccaa gatgtagcag cttcgttgac 360

acaagcagaa aaacaagtta gtcaagagaa aaatactaat attggtaatc gaaatcaaga 420

gggaaaattt gtcgatttgc cttttgcaca ggtgggtaaa gttattgtca ggtttccacc 480

agaagcatca ggttatttgc acatcggcca cgccaaagct gcccttctca accaacacta 540

tcaggaagct ttccagggga aacttatttt acgtttcgat gatactaatc ctgccaaaga 600

aaatgtgcat tttgaaaacg ttattctgga agatgtagca atgttacaga ttaaacccga 660

catattcaca tatacctcag attactttga acttatgtta gagttatgtg aaaaaatgat 720

gaaagaagga aatgcctatg tagatgatac tgaaccagag gaaatgaaaa ctcaaagaga 780

acaaaaaatc 790

<210>47

<211>1491

<212>DNA

<213>Diabrotica virgifera

<400>47

gtgagtagtg aaaaaatatg gccggagcaa tgttatttga aagagccccg tctgtttctt 60

ctcaacacga gaagctttta aaattatcca gggattcaga tgatgaagat caaaatattg 120

tcaataaaga acaagacatc cttaatcttg gtgagaaata taaaaaggag ggcaaggcaa 180

aggaactcgc agagcttata aaggccacca ggcctttttt tgagtttaat aagcaaagca 240

aaagctgcta aattggtaag gtctctagtt gactacttcc tagatttaga agctggtatt 300

ggtatcgaag tacaactctg caaagaatgt atagaatggg caaaggaaga gagaagaact 360

tttctacgtc agtcccttga agccaggctg attgctcttt attttgacac tggaatgtac 420

agcgaagcgc tacaactgga atcgacctta ctgaaagagt taaaaaagtt ggatgacaaa 480

aatttgttgg tggaagtaca actacttgaa agcaaaacat atcatgcttt aagtaattta 540

ccgaaggcta gagctgcact aacttcagca cgaaccacag ctaacgctat ctattgccct 600

ccaaagatgc aggcagcttt agatttacaa tccggagtat tacacgctgc agatgagaag 660

gatttcaaaa cagcttattc ttatttttat gaagcttttg aaggttttga tagtgttgaa 720

tccccaaaag ccctcacagc ccttaaatac atgcttttgt caaaaatcat gataaacagt 780

ccggaagacg tccaacaaat cgttagtggt aaattggcct ttaaatacgc tggtcaagat 840

atagaggcta tgaaatcagt tgcacaggct gcacacaaaa gatcactagc agatttccaa 900

caagctgtaa aacagtttaa acatgaatta gaagatgatg ttattgttag agcacattta 960

ggaacgttgt atgacaatat gttggagcag aacctttgca gaattattga accatattct 1020

agggtacaag tagagtatgt agcaaaaacc ataaaactcc cagtactaca agtagagaag 1080

aaattgtcac agatgatttt agatgccaag tttcatggaa ttttggacca aggcgaaggt 1140

gtcctcatag tattcgaaga aagtgcagta gacaaaacat atgaaatggc cctagaaacg 1200

atccagagta tgagcaaagt agtagatact ctttatcaaa aagcaaagaa attgtcatag 1260

gtataatgta gccgagaatg ttatgaaaat aataaaatga ctgtaaattt ttcagaacta 1320

gattttttgt agacactgtg tttatataac ctatttaatt tgcttttgtt aattcttgtc 1380

tttttaagga ttcttataac tcatagtttt cggagcttga gggttttgga tgtgatggag 1440

tttttttgtt aatacagtat agatattttt taagatactt aaagcttatc t 1491

<210>48

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>48

gcaaagaatg tatagaatgg gcaaaggaag agag 34

<210>49

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>49

ggttctgctc caacatattg tcatacaacg 30

<210>50

<211>675

<212>DNA

<213>Diabrotica virgifera

<400>50

gcaaagaatg tatagaatgg gcaaaggaag agagaagaac ttttctacgt cagtcccttg 60

aagccaggct gattgctctt tattttgaca ctggaatgta cagcgaagcg ctacaactgg 120

aatcgacctt actgaaagag ttaaaaaagt tggatgacaa aaatttgttg gtggaagtac 180

aactacttga aagcaaaaca tatcatgctt taagtaattt accgaaggct agagctgcac 240

taacttcagc acgaaccaca gctaacgcta tctattgccc tccaaagatg caggcagctt 300

tagatttaca atccggagta ttacacgctg cagatgagaa ggatttcaaa acagcttatt 360

cttattttta tgaagctttt gaaggttttg atagtgttga atccccaaaa gccctcacag 420

cccttaaata catgcttttg tcaaaaatca tgataaacag tccggaagac gtccaacaaa 480

tcgttagtgg taaattggcc tttaaatacg ctggtcaaga tatagaggct atgaaatcag 540

ttgcacaggc tgcacacaaa agatcactag cagatttcca acaagctgta aaacagttta 600

aacatgaatt agaagatgat gttattgtta gagcacattt aggaacgttg tatgacaata 660

tgttggagca gaacc 675

<210>51

<211>942

<212>DNA

<213>Diabrotica virgifera

<400>51

gaagaacata cgtccagact ccagacaaac ctttcaacaa atttagaaaa catttatata 60

aaaatatcaa cagttctgct tcagaaatac ataaaatgta atttaactta agtcttgtaa 120

gatatcaatt gttacacacg cgctttggaa cgatgacaga gttttagtaa ttggttaatg 180

agtttataaa tcttcgtcat catcgggcag tgcagtttct gaggcttctt taagatcctt 240

ttcgatttgt tgctgccatt ggggatccat ctggacttct ggaggaagca aggcaggtgc 300

agctacgaat tctaagttgg gatctccaat gagtttccta gccaaccaca agaagggttt 360

ttcaaagttg tagttggatt ttgcagagat gtcatagtat tgtaaattct tcttcctatg 420

gaagacaatg ctctttgcct taactttcct atctttgatg tcaactttgt taccacacaa 480

tacaattggg atattttcac aaactctaac aagatctctg tgccagtttg gaacattttt 540

gtaagtgact ctagatgtaa catcaaacat aattatggca cattgtcctt ggatgtagta 600

tccatcacgc agaccaccaa acttttcttg gcctgctgtg tcccatacgt taaaacgaat 660

ttgtcctcta tttgtgtgga aaactaaagg gtgaacttct actcctaagg ttgctacata 720

tttcttttcg aattcaccag tcatgtgtct ttttacaaag gtagttttgc cagtacctcc 780

atctccgacc aagacgcatt tgaaggtggg catatcttgt tcgccagcca tgattttttg 840

aatttaaact tgtgaataaa aagtactagg aaacaccgat ggcacaaaaa atcacggatt 900

acttcagctt ggttccaaag tcgttagagt tcacaatggc gt 942

<210>52

<211>22

<212>DNA

<213>Diabrotica virgifera

<400>52

cttcgtcatc atcgggcagt gc 22

<210>53

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>53

cgtcttggtc ggagatggag gtactg 26

<210>54

<211>605

<212>DNA

<213>Diabrotica virgifera

<400>54

cttcgtcatc atcgggcagt gcagtttctg aggcttcttt aagatccttt tcgatttgtt 60

gctgccattg gggatccatc tggacttctg gaggaagcaa ggcaggtgca gctacgaatt 120

ctaagttggg atctccaatg agtttcctag ccaaccacaa gaagggtttt tcaaagttgt 180

agttggattt tgcagagatg tcatagtatt gtaaattctt cttcctatgg aagacaatgc 240

tctttgcctt aactttccta tctttgatgt caactttgtt accacacaat acaattggga 300

tattttcaca aactctaaca agatctctgt gccagtttgg aacatttttg taagtgactc 360

tagatgtaac atcaaacata attatggcac attgtccttg gatgtagtat ccatcacgca 420

gaccaccaaa cttttcttgg cctgctgtgt cccatacgtt aaaacgaatt tgtcctctat 480

ttgtgtggaa aactaaaggg tgaacttcta ctcctaaggt tgctacatat ttcttttcga 540

attcaccagt catgtgtctt tttacaaagg tagttttgcc agtacctcca tctccgacca 600

agacg 605

<210>55

<211>763

<212>DNA

<213>Diabrotica virgifera

<400>55

gtcatactca cggttgtttt tgtaaaactt gtaggtaacg aattttcgtt tttagtgaat 60

taacaaaaca acagagtgat tttccaacaa gaataactta tttattagtt tcattaattt 120

gataaacaat aaaatggata gacttcttcg actaggaggt ggaatgccag gtttaactca 180

ggcaccgccg ccttctgatg ctcctgttgt ggatactgcc gaacaagtat acatatcctc 240

cttagcacta ttaaaaatgt tgaagcacgg ccgagctgga gtacccatgg aagtaatggg 300

tcttatgtta ggagaattcg tagatgacta taccgttcgt gtaatcgatg tatttgctat 360

gcctcagact ggtaccggtg tcagtgtaga agctgttgat ccagtttttc aagcaaaaat 420

gcttgacatg ttaagacaga ctggtaggcc agaaatggtt gtggggtggt atcattccca 480

tccaggtttc ggatgttggt tgtctggagt ggatatcaac acccagcaaa gttttgaggc 540

tctctcggaa agagctgtag cagtagtagt tgatccaatc cagtcagtga aaggcaaagt 600

cgttattgat gcctttaggt taataaatcc gaatatgatg gtattgggcc aagaaccgcg 660

ccaaactaca tccaacttag gacatctaca gaaaccatca gtccaggctt tgattcatgg 720

tctcaatcgg cattactact ccatcagtat taattacaga aag 763

<210>56

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>56

cgactaggag gtggaatgcc agg 23

<210>57

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>57

caaagcctgg actgatggtt tctgtag 27

<210>58

<211>564

<212>DNA

<213>Diabrotica virgifera

<400>58

cgactaggag gtggaatgcc aggtttaact caggcaccgc cgccttctga tgctcctgtt 60

gtggatactg ccgaacaagt atacatatcc tccttagcac tattaaaaat gttgaagcac 120

ggccgagctg gagtacccat ggaagtaatg ggtcttatgt taggagaatt cgtagatgac 180

tataccgttc gtgtaatcga tgtatttgct atgcctcaga ctggtaccgg tgtcagtgta 240

gaagctgttg atccagtgtt tcaagcaaaa atgcttgaca tgttaagaca gactggtagg 300

ccagaaatgg ttgtggggtg gtatcattcc catccaggtt tcggatgttg gttgtctgga 360

gtggatatca acacccagca aagttttgag gctctctcgg aaagagctgt agcagtagta 420

gttgatccaa tccagtcagt gaaaggcaaa gtcgttattg atgcctttag gttaataaat 480

ccgaatatga tggtattggg ccaagaaccg cgccaaacta catccaactt aggacatcta 540

cagaaaccat cagtccaggc tttg 564

<210>59

<211>1156

<212>DNA

<213>Diabrotica virgifera

<400>59

cacaaattgg agacaagttc gcgtctaggc acggacaaaa gggaacgtgc ggtatacaat 60

accgtcaaga ggatatgatt tttagtgctg aaggtattac gccagacatt atcattaatc 120

ctcacgctat cccctcccgt atgacgattg gtcacttgat cgagtgcatc caaggtaagg 180

tatcatccaa taaaggagaa atcggtgatg ctacaccttt caacgatgcc gtcaacgtac 240

agaaaatctc cactttgtta caagaatatg gttatcaact cagaggcaac gaagtaatgt 300

acaacggcca tactggacgt aagataaatg ctcagatttt cttaggacct acctattatc 360

aaagattgaa gcacatggtg gacgataaga ttcattcaag agctagagga ccgttgcaga 420

ttctcgtcag acagcctatg gagggtcgtg caagagacgg cggtctacgt ttcggtgaga 480

tggaacgaga ttgtcaaatt tcacacggtg cggcacaatt cttaagagag aggctgtttg 540

aagtttccga tccttataga atccatattt gcaacttctg tggtttgata gctatcgcca 600

atttgcgtaa caacaccttt gaatgtaagg gttgcaaaaa taagacgcag atatcgcaag 660

ttagactgcc ttatgctgcg aaattactgt ttcaagagct aatgtcgatg aatatagcgc 720

cgagacttat ggtactgtag tgtttttaag taaataaata aatgtcctta tctaagagga 780

ttttatattg aaataaacca ttaaaatttt cccaccacac cccaccacca ccacccacac 840

aaaccaacaa acaaaaaaaa aacaaaggaa gaaaaaacaa aacggggggg ggcgctttaa 900

agggatccaa ggtttaaggc ccgcggggag gggacagcct aaatcttttt ttataagggc 960

ccccaaaaat tcaatttccc cggggccgcg cttttttcaa acagcccggg gacggggaaa 1020

aaacccctgg ggggtttccc caattttaat ctcccctttg gcaaaaccct tccccccttt 1080

ttttcccccc cctggggggg gaaaaaaaag aagaaaaaag ggcccccgcc cccccacaat 1140

tctcccccct cctccc 1156

<210>60

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>60

gtctaggcac ggacaaaagg gaacg 25

<210>61

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>61

gacaatctcg ttccatctca ccgaaac 27

<210>62

<211>677

<212>DNA

<213>Diabrotica virgifera

<400>62

gtctaggcac ggacaaaagg gaacgtgcgg tatacaatac cgtcaagagg atatgatttt 60

tagtgctgaa ggtattactc cagacattat catcaatcct cacgctatcc cctcccgtat 120

gacgattggt cacttgatcg agtgcatcca aggtaaggta tcatccaata aaggagaaat 180

cggtgatgct acacctttca acgatgccgt caacgtacag aaaatctcca ctttgttaca 240

agaatatggt tatcaactca gaggcaacga agtaatgtac aacggccaca ctggacgtaa 300

gataaatgct cagattttct taggacctac ttactatcaa agattgaagc acatggtgga 360

cgataagatc cattcaagag ctagaggacc gttgcagatt ctcgtcagac agcctatgga 420

gggccgtgca agagacggtg gtctacgttt cggtgagatg gaacgagatt gtcaaatttc 480

acacggtgct gcacaattct taagagagag gctgtttgaa gtttccgatc cttatagaat 540

ccatatttgc aacttctgtg gtttgatagc tatcgccaat ttgcgtaaca acacctttga 600

atgtaagggt tgcaaaaata agacacagat atcgcaagtt agactgcctt atgctgcgaa 660

attactgttt caagagc 677

<210>63

<211>1156

<212>DNA

<213>Diabrotica virgifera

<400>63

cacaaattgg agacaagttc gcgtctaggc acggacaaaa gggaacgtgc ggtatacaat 60

accgtcaaga ggatatgatt tttagtgctg aaggtattac gccagacatt atcattaatc 120

ctcacgctat cccctcccgt atgacgattg gtcacttgat cgagtgcatc caaggtaagg 180

tatcatccaa taaaggagaa atcggtgatg ctacaccttt caacgatgcc gtcaacgtac 240

agaaaatctc cactttgtta caagaatatg gttatcaact cagaggcaac gaagtaatgt 300

acaacggcca tactggacgt aagataaatg ctcagatttt cttaggacct acctattatc 360

aaagattgaa gcacatggtg gacgataaga ttcattcaag agctagagga ccgttgcaga 420

ttctcgtcag acagcctatg gagggtcgtg caagagacgg cggtctacgt ttcggtgaga 480

tggaacgaga ttgtcaaatt tcacacggtg cggcacaatt cttaagagag aggctgtttg 540

aagtttccga tccttataga atccatattt gcaacttctg tggtttgata gctatcgcca 600

atttgcgtaa caacaccttt gaatgtaagg gttgcaaaaa taagacgcag atatcgcaag 660

ttagactgcc ttatgctgcg aaattactgt ttcaagagct aatgtcgatg aatatagcgc 720

cgagacttat ggtactgtag tgtttttaag taaataaata aatgtcctta tctaagagga 780

ttttatattg aaataaacca ttaaaatttt cccaccacac cccaccacca ccacccacac 840

aaaccaacaa acaaaaaaaa aacaaaggaa gaaaaaacaa aacggggggg ggcgctttaa 900

agggatccaa ggtttaaggc ccgcggggag gggacagcct aaatcttttt ttataagggc 960

ccccaaaaat tcaatttccc cggggccgcg cttttttcaa acagcccggg gacggggaaa 1020

aaacccctgg ggggtttccc caattttaat ctcccctttg gcaaaaccct tccccccttt 1080

ttttcccccc cctggggggg gaaaaaaaag aagaaaaaag ggcccccgcc cccccacaat 1140

tctcccccct cctccc 1156

<210>64

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>64

gtctaggcac ggacaaaagg gaacg 25

<210>65

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>65

gctcttgaaa cagtaatttc gcagcat 27

<210>66

<211>677

<212>DNA

<213>Diabrotica virgifera

<400>66

gtctaggcac ggacaaaagg gaacgtgcgg tatacaatac cgtcaagagg atatgatttt 60

tagtgctgaa ggtattactc cagacattat catcaatcct cacgctatcc cctcccgtat 120

gacgattggt cacttgatcg agtgcatcca aggtaaggta tcatccaata aaggagaaat 180

cggtgatgct acacctttca acgatgccgt caacgtacag aaaatctcca ctttgttaca 240

agaatatggt tatcaactca gaggcaacga agtaatgtac aacggccaca ctggacgtaa 300

gataaatgct cagattttct taggacctac ttactatcaa agattgaagc acatggtgga 360

cgataagatc cattcaagag ctagaggacc gttgcagatt ctcgtcagac agcctatgga 420

gggccgtgca agagacggtg gtctacgttt cggtgagatg gaacgagatt gtcaaatttc 480

acacggtgct gcacaattct taagagagag gctgtttgaa gtttccgatc cttatagaat 540

ccatatttgc aacttctgtg gtttgatagc tatcgccaat ttgcgtaaca acacctttga 600

atgtaagggt tgcaaaaata agacacagat atcgcaagtt agactgcctt atgctgcgaa 660

attactgttt caagagc 677

<210>67

<211>919

<212>DNA

<213>Diabrotica virgifera

<400>67

cggacgcgtg ggttgggatt taacaaatac gtcgttctaa atttctaaaa ctgcatacaa 60

aaaatcctta aaaaactagt gaagattact aaagtgcaat agatacaaaa acaatatttt 120

tacgtattca ccataataat ataaacgtag aaaatgaatc ccgagtatga ttatttattc 180

aaacttctgc tgattggaga ttcaggagta ggaaaatctt gtcttctact gagatttgca 240

gatgatacct acacagaaag ctatattagt accattggcg tagattttaa aatcaggaca 300

atcgatttag atggaaagac aattaaattg caaatttggg atacagcagg tcaggaaagg 360

tttagaacga ttacatcaag ttattaccga ggagcacatg gtattattgt agtgtacgat 420

tgcacagacc aagattcatt caataacgtt aaacaatggc tcgaagaaat cgaccgttat 480

gcgtgtgaca atgtaaacaa attactggta gggaataaaa gcgatttgac aactaagaaa 540

gttgtcgact tcactacagc caaggagtat gccgaccaat tgggtatacc atttttggaa 600

acctcagcta agaatgcaac caatgtagaa caggccttta tgactatggc cgctgaaata 660

aaaaatagag taggacctcc atcttctgcg gtagaccaag gaaataaggt taggttcgat 720

caaagtcgcc cagtcgaaac aaccaaatcc ggttgctgct gaaaatattt agttctcttt 780

ggttgaaggt ggtcactcat gtcatctctt ttgtacggta gcagcgctac agttactctt 840

attctagtaa tatccgtctg tatgtatagt tattttaaag tttttgtttg attttcactt 900

ttaaattttt tttttcaaa 919

<210>68

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>68

caaacttctg ctgattggag attcagg 27

<210>69

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>69

cttggtctac cgcagaagat ggagg 25

<210>70

<211>521

<212>DNA

<213>Diabrotica virgifera

<400>70

caaacttctg ctgattggag attcaggagt aggaaaatct tgtcttctac tgagatttgc 60

agatgatacc tacacagaaa gctatattag taccattggc gtagatttta aaatcaggac 120

aatcgattta gatggaaaga caattaaatt gcaaatttgg gatacagcag gtcaggaaag 180

gtttagaacg attacatcaa gttattaccg aggagcacat ggtattattg tagtgtacga 240

ttgcacagac caagattcat tcaataacgt taaacagtgg ctcgaagaaa tcgaccgtta 300

tgcgtgtgac aatgtaaaca aattactggt agggaataaa agcgatttga caactaagaa 360

agttgtcgac ttcactacag ccaaggagta tgccgaccaa ttgggtatac catttttgga 420

aacctcagct aagaatgcaa ccaatgtaga acaggccttt atgactatgg ccgctgaaat 480

aaaaaataga gtaggacctc catcttctgc ggtagaccaa g 521

<210>71

<211>755

<212>DNA

<213>Diabrotica virgifera

<400>71

cgttaatcta gaaatcagtc tttttatgtt cagaggttga aaataaaacc tcaaaatggt 60

aaacggtagg ataccgtcgg ttcattctaa aacctatgta acgcctaggc gtccatatga 120

gaaggcccgt ttagatcaag aattgaaaat catcggagcc tttggtctcc gaaacaaacg 180

tgaagtatgg agagtaaaat acactctagc caaaatccgt aaggccgcca gagaactgct 240

tactctagaa gagaaagaac ccaaaaggtt gtttgaaggt aatgcactcc tacgtcgttt 300

ggttcgtatt ggagtactag acgagaacag aatgaagctc gattacgtat tgggtctcaa 360

gattgaagat ttcttggaaa gacgtctgca aacacaagtc ttcaaatctg gtctagctaa 420

gtcaatccat catgctaggg tattgatcag acaacgacac attcgggtcc gcaaacaagt 480

tgtgaacatt ccctccttca tcgtccgttt ggattcacaa aaacacatcg acttctctct 540

gaaatcacct ttgggaggcg gtcgtccagg acgtgttaag aggaagaacc tcaccaagaa 600

gagcggtggt ggaggtgcag ctgaagaaga ggaagactaa gctgtatgtc gtgctagttg 660

tactagtact tacatattaa tatacttatg tattaaaaaa aaaaaaaaaa aaaaaaaaaa 720

aaaaaagaaa aaagaaaaaa aaaaaaaaaa aaaaa 755

<210>72

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>72

gtttagatca agaattgaaa atcatcggag c 31

<210>73

<211>21

<212>DNA

<213>Diabrotica virgifera

<400>73

gcacctccac caccgctctt c 21

<210>74

<211>490

<212>DNA

<213>Diabrotica virgifera

<400>74

gtttagatca agaattgaaa atcatcggag cctttggtct ccgaaacaaa cgtgaagtat 60

ggagagtaaa atacactcta gccaaaatcc gtaaggccgc cagagaactg cttactctag 120

aagagaaaga acccaaaagg ttgtttgaag gtaatgcact cctacgtcgt ttggttcgta 180

ttggagtact agacgagaac agaatgaagc tcgattacgt attgggtctc aagattgaag 240

atttcttgga aagacgtctg caaacacaag tcttcaaatc tggtctagct aagtcaatcc 300

atcatgctag ggtattgatc agacaacgac acattcgggt ccgcaaacaa gttgtgaaca 360

ttccctcctt catcgtccgt ttggattcac aaaaacacat cgacttctct ctgaaatcac 420

ctttgggagg cggtcgtcca ggacgtgtta agaggaagaa cctcaccaag aagagcggtg 480

gtggaggtgc 490

<210>75

<211>857

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(818)

＜223〉n=a, c, g or t/u

<400>75

catctttctg tctgttcccg gcgttctttg aaaaagtttt caataatact ctaaaaaatg 60

gcagacgctg atgatctatt agattatgaa gatgaggaac agacagaaca aaccgcaact 120

gaaacggcaa ctacagaggt acagaaaaag ggtgtcaagg gcacatatgt atcaatacac 180

agttctgggt ttagagattt tctgttaaaa ccagcaattc tcagagctat agtggactgc 240

gggttcgaac atccttcaga agttcaacat gaatgtattc ctcaagctgt cattggcatg 300

gatattctgt gccaagctaa atccggtatg ggaaaaacgg ctgtttttgt attagctaca 360

ctccaagtaa tagatcctac agaaaatgtt gtatatgttc tcgtcatgtg ccataccaga 420

gagttagcct tccagataag caaagagtac gaacgtttca gtaaatatat gcccaatatt 480

aaagtagggg tcttctttgg tggcttgcct atccagaaag atgaggaaac gttaaaaaat 540

aattgcccgc atatcgttgt gggtactcca ggaagaattt tagcattggt cagatcgaaa 600

aaacttaatc tcaaacatct aaagcatttt attttggatg aatgtgataa aatgttggag 660

ttattagaca tgagacgtga tgttcaagaa atatatcgta acactcccca cgaaaaacaa 720

gtcatgatgt tcagtgccac cttaagtaaa gaaattagac cagtttgcaa gaaatttatg 780

caagatccca tggaagtata tgttgacgac gaagctanat taacattgca tggtctacag 840

caacattatg taaaact 857

<210>76

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>76

gcagacgctg atgatctatt agattatgaa gatg 34

<210>77

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>77

cttgaacatc acgtctcatg tctaataact cc 32

<210>78

<211>627

<212>DNA

<213>Diabrotica virgifera

<400>78

gcagacgctg atgatctatt agattatgaa gatgaggaac agacagaaca aaccgcaact 60

gaaacggcaa ctacagaggt acagaaaaag ggtgtcaagg gcacatatgt atcaatacac 120

agttctgggt ttagagattt tctgttaaaa ccagcaattc tcagagctat agtggactgc 180

gggttcgaac atccttcaga agttcaacat gaatgtattc ctcaagctgt cattggcatg 240

gatattctgt gccaagctaa atccggtatg ggaaaaacgg ctgtttttgt attagctaca 300

ctccaagtaa tagatcctac agaaaatgtt gtatatgttc tcgtcatgtg ccataccaga 360

gagttagcct tccagataag caaagagtac gaacgtttca gtaaatatat gcccaatatt 420

aaagtagggg tcttctttgg tggcttgcct atccagaaag atgaggaaac gttaaaaaat 480

aattgcccgc atatcgttgt gggtactcca ggaagaattt tagcattggt cagatcgaaa 540

aaacttaatc tcaaacatct aaagcatttt attttggatg aatgtgataa aatgttggag 600

ttattagaca tgagagtgat gttcaag 627

<210>79

<211>847

<212>DNA

<213>Diabrotica virgifera

<400>79

cccacgcgtc cgtatctaac caggcagcta aattaaacaa ctttactgat aatgcagaac 60

tgtggctctt aaactacgaa gacgatactt tcaaagacca agtacaagct ttatgggagc 120

aagtgaagcc tttataccta gaacttcacg cctatgtgag gttcaagcta agacaaaaat 180

acggagatgt tgtcccggag aaaggaccta ttcctgctca tttgttagga aatatgtggg 240

cacaaacatg gaacaatatt gcaagcttca cattaccata tccagatgtt gttgattatg 300

atataactgc agaaatgttg aaacagaact tcacagcgaa gaagatattt gaaacggccg 360

aaagtttttt caagtccatc aatttaacag ctatgcccga tctgttctgg cagagatcaa 420

tcatagagaa gccgaaggat agggaaatcg tttgtcatgc ctcagcctgg gacttctacg 480

atggaaagga cttcaggatc aaaatgtgta ctgacgttaa ttatgaggat atgaacacag 540

ctcaccacga aatgggacac atagagtact ttttacagta ccgcaatcag cctactccat 600

accggactgg agctaatgat ggattccacg aagctgttgg agacctgatt tctttatcat 660

tcagttccac caaacatttg cggaaaattg gactaataac atccaaagct gataacaatg 720

aacagcttct caacaactta tacaagattg gtttagaaaa aatcgcattc ttgccatttc 780

gatacttgat cgacttgtgc cgatgggata tctttgcacg aaaaactact cctgaaaact 840

accaact 847

<210>80

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>80

gcgtccgtat ctaaccaggc agc 23

<210>81

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>81

ggtggaactg aatgataaag aaatcaggtc 30

<210>82

<211>666

<212>DNA

<213>Diabrotica virgifera

<400>82

gcgtccgtat ctaaccaggc agctaaatta aacaacttta ctgataatgc agaactgtgg 60

ctcttaaact acgaagacga tactttcaaa gaccaagtac aagctttatg ggagcaagtg 120

aagcctttat acctagaact tcacgcctat gtgaggttca agctaagaca aaaatacgga 180

gatgttgtcc cggagaaagg acctattcct gctcatttgt taggaaatat gtgggcacaa 240

acatggaaca atattgcaag cttcacatta ccatatccag atgttgttga ttatgatata 300

actgcagaaa tgttgaaaca gaacttcaca gcgaagaaga tatttgaaac ggccgaaagt 360

tttttcaagt ccatcaattt aacagctatg cccgatctgt tctggcagag atcaatcata 420

gagaagccga aggataggga aatcgtttgt catgcctcag cctgggactt ctacgatgga 480

aaggacttca ggatcaaaat gtgtactgac gttaattatg aggatatgaa cacagctcac 540

cacgaaatgg gacacataga gtacttttta cagtaccgca atcagcctac tccataccgg 600

actggagcta atgatggatt ccacgaagct gttggagacc tgatttcttt atcattcagt 660

tccacc 666

<210>83

<211>928

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(750)..(835)

＜223〉n=a, c, g or t/u

<400>83

cagatgtgtg gtatgcacat cgtcgtacac gctgatgagt tgcaagattt gattaattat 60

tatcaagata gaggatactt tgaagaatta atcggcctat tggaagcagc attgggcttg 120

gaaagggcac acatgggtat gttcaccgaa ttagcaattt tgtattccaa atacaagccc 180

gccaaaatgc gcgaacatct ggaactcttc tggtctcgag tgaatatccc caaagttctt 240

agggcagctg aacaagcaca tttgtgggca gagttagtgt ttttgtatga caaatatgaa 300

gaatatgata acgctgtgtt agccatgatg gctcatccca ccgaagcttg gcgcgaaggt 360

catttcaagg atattattac aaaagttgcc aatatcgaac tttattatag agctattcaa 420

ttttacttgg attacaaacc gctgttgtta aatgaccttt tgttggtgtt ggcacccagg 480

atggatcata ctagggctgt ttctttcttt acaaaaacag gacacttaca gttggttaaa 540

tcctatctcc ggtctgtgca aaatttgaac aacaaagcta tcaacgaggc cctcaactct 600

ctacttatcg aagaagaaga cttccagggt ctgagaacgt ccatcgacgc cttcgataac 660

ttcgacaaca tcggcttggc tcagaaattg gagaagcacg aactgacgga gttcagacgc 720

atcgccgcct acttgtacaa aggcaacaan ccggtggaag cagagcgtag aactgtgcaa 780

aaaggataga ctatttagag acgctatgga gtacacttct gaatctagaa atcangaaat 840

tgccgaaaga gtgctggcat gggtcttgga taggaaggcc tatgattgtt tttcggcatg 900

tttgtatccc tgctacgact tgttacgg 928

<210>84

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>84

gcacatcgtc gtacacgctg atgag 25

<210>85

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>85

cgtgcttctc caatttctga gccaag 26

<210>86

<211>686

<212>DNA

<213>Diabrotica virgifera

<400>86

gcacatcgtc gtacacgctg atgagttgca agatttgatt aattattatc aagatagagg 60

atactttgaa gaattaatcg gcctattgga agcagcattg ggcttggaaa gggcacacat 120

gggtatgttc accgaattag caattttgta ttccaaatac aagcccgcca aaatgcgcga 180

acatctggaa ctcttctggt ctcgagtgaa tatccccaaa gttcttaggg cagctgaaca 240

agcacatttg tgggcagagt tagtgttttt gtatgacaaa tatgaagaat atgataacgc 300

tgtgttagcc atgatggctc atcccaccga agcttggcgc gaaggtcatt tcaaggatat 360

tattacaaaa gttgccaata tcgaacttta ttatagagct attcaatttt acttggatta 420

caaaccgctg ttgttaaatg accttttgtt ggtgttggca cccaggatgg atcatactag 480

ggctgtttct ttctttacaa aaacaggaca cttacagttg gttaaatcct atctccggtc 540

tgtgcaaaat ttgaacaaca aagctatcaa cgaggccctc aactctctac ttatcgaaga 600

agaagacttc cagggtctga gaacgtccat cgacgccttc gataacttcg acaacatcgg 660

cttgtctcag aaattggaga agcacg 686

<210>87

<211>999

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(753)..(827)

＜223〉n=a, c, g or t/u

<400>87

aaaaacgaag aaagtacgtt aatatggttc cagggctcca acaagacgag ctattcttac 60

tgggtcgaga acattttaaa atttttggat aagtactacg ttcccagcaa aatagctaaa 120

ggaaatggcc aaattaaaac atgctctcat cacgactatc ctactagtgg agaagtatgc 180

gaagtcgatg tcagagattg ggaagaatgc aacagggatc aattctttaa ttatcacaag 240

aattctccat gtatttttat taaattgaac aaaatatata actgggagcc aatgtattac 300

gatgatccat acgatttacc tgaggaaatg ccggataatc tgaagcaaca tataaggggt 360

atcaacaata ctagagagtt gagaaacgtt tgggtctcat gtcaaggaga aaatcctgct 420

gatgtagaat acttgggccc cattacatac tatccccgaa gcgtacaggg cttccctggt 480

tactattttc cgtacttgaa ctctgaaggt tacctaagtc ctttagtagc agttaaattt 540

gaaagaccag tggctggtat cgttattaac gtagagtgca gagcctgggc aaggaacata 600

ctttacaaca gagccgatag aacgggctct gtgcatttcg agctgctaat tgattaaata 660

ttattttacc caattggaca atagaagaat ccttcaggac tttttaattc aaatattata 720

tagtaataga aatgttgcct tanaatangg ggcttttata aaatagccga tcgatcacat 780

anagtaagct ataagaagta cattccaata tgaaccaccc ataccanata tttttataca 840

ttttataaat ttatataact tttttaatct tactgaaata tccttttggc atccattgtt 900

gcaatttttg tttctagatt aaaatatatt gggttccata ttatttttaa agggatgaat 960

aatttttgga aaagaaaaaa atttttgttt tggtggggg 999

<210>88

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>88

gaagaaagta cgttaatatg gttccagggc tc 32

<210>89

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>89

gtatgttcct tgcccaggct ctgc 24

<210>90

<211>595

<212>DNA

<213>Diabrotica virgifera

<400>90

gaagaaagta cgttaatatg gttccagggc tccaacaaga cgagctattc ttactgggtc 60

gagaacattt taaaattttt ggataagtac tacgttccca gcaaaatagc taaaggaaat 120

ggccaaatta aaacatgctc tcatcacgac tatcctacta gtggagaagt atgcgaagtc 180

gatgtcagag attgggaaga atgcaacagg gatcaattct ttaattatca caagaattct 240

ccatgtattt ttattaaatt gaacaaaata tataactggg agccaatgta ttacgatgat 300

ccatacgatt tacctgagga aatgccggat aatctgaagc aacatataag gggtatcaac 360

aatactagag agttgagaaa cgtttgggtc tcatgtcaag gagaaaatcc tgctgatgta 420

gaatacttgg gccccattac atactatccc cgaagcgtac agggcttccc tggttactat 480

tttccgtact tgaactctga aggttaccta agtcctttag tagcagttaa atttgaaaga 540

ccagtggctg gtatcgttat taacgtagag tgcagagcct gggcaaggaa catac 595

<210>91

<211>1560

<212>DNA

<213>Diabrotica virgifera

<400>91

cggacgcgtg ggaaacacat ggatttcgtc gtctcgtcat aagttgaaat acaagtgaaa 60

atatttgtga tatttagttt tagtgtgcca aaataaaaat ggatgacgac caacaatttt 120

gtttacggtg gaacaaccat caatcgacat tggtagccgt ttttgacacg ttattggaga 180

acggcacatt agtagactgt actttggcag cagaaggaaa atgcctaaac gcacacaaag 240

tagttttgtc tgcatgtagt ccatacttcg aggcgctgct gtcaaagcac tttgacaagc 300

atcccattct tattcttaag gacgttaagt tccaagaact aaaagccatg atggactata 360

tgtacagggg ggaagtgaac atatctcaag atcaattagg cgcgctttta aaagccgctg 420

aatctttgca aattaagggt ttatcagaca ataggaaagg ggaaactgaa cgaaaaccca 480

ctcctgctcc tccaccaaag agcccacagc caacgaccct gccgaaggtc cagggtttga 540

caattgagca aagagctcgg gaggattcca gggagggaag catgtcacca gggcctcgaa 600

aaaagaaaag gttaaggcgg aaaagtgagg aattagacaa ccacgatgcc tctaattctt 660

cagaatctca cagtctaccc gcccaaagca ttcctacgtt acctgctatt tcaaaaatga 720

acatgaacgc cgatgtgcct gaacaaatag aaactaaatc agagatactc acaaggcata 780

aacagacaga tgacatgccc caagttccta ttataaaaga aaagattgaa acccacacag 840

aactgatgtt ggaaccaaag tctgaatatg tagaagaaat gaatgaagat agtattgagg 900

atttgacact ggatgatgac gatttaagta atatggaaca ggtagatgat caagcaggac 960

ctagtcatgg agcaggagaa ggatcaagtc aaggttttgg aggttggcat atgggtaacc 1020

aaagtcaaga tgaagtattt ctggccgcgc aagaggccgt aggtgcacat cgagactcac 1080

aaggtaagat ttttgttttc aattgtaaag gcttgtctac tgttctgcaa ctgacttgtt 1140

aaaggagtgt atacttcaac cactcctttg gtcatgagac ctgttagtac ttacaaacta 1200

caatggtagt tgttgaagtt ctttacagta gacaagtttc attcacttgc aagtggtatg 1260

gcaaaattta acaccctgac ttccagtctc tggtttaaca ttttatttta ttttacctaa 1320

cgggtagtcc ttggtttctc ctgttgctta acacttagct ttttatttta ctagatgtag 1380

tagtagttat ttttggtttt acccatcacc tttctccctc cctcaagatt gacagcactc 1440

cttcatcaat ttttcatttg gaggtaccag ccagccctac aggtcaaccc aaaaccacta 1500

gtattttaaa tctaaattgt tttgagagaa gtttcataga tgatacctta ctattaataa 1560

<210>92

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>92

cgtgggaaac acatggattt cgtc 24

<210>93

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>93

ggaacttggg gcatgtcatc tgtc 24

<210>94

<211>802

<212>DNA

<213>Diabrotica virgifera

<400>94

cgtgggaaac acatggattt cgtcgtctcg tcataagttg aaatacaagt gaaaatattt 60

gtgatattta gttttagtgt gccaaaataa aaatggatga cgaccaacaa ttttgtttac 120

ggtggaacaa ccatcaatcg acattggtag ccgtttttga cacgttattg gagaacggca 180

cattagtaga ctgtactttg gcagcagaag gaaaatgcct aaacgcacac aaagtagttt 240

tgtctgcatg tagtccatac ttcgaggcgc tgctgtcaaa gcactttgac aagcatccca 300

ttcttattct taaggacgtt aagttccaag aactaaaagc catgatggac tatatgtaca 360

ggggggaagt gaacatatct caagatcaat taggcgcgct tttaaaagcc gctgaatctt 420

tgcaaattaa gggtttatca gacaatagga aaggggaaac tgaacgaaaa cccactcctg 480

ctcctccacc aaagagccca cagccaacga ccctgccgaa ggtccagggt ttgacaattg 540

agcaaagagc tcgggaggat tccagggagg gaagcatgtc accagggcct cgaaaaaaga 600

aaaggttaag gcggaaaagt gaggaattag acaaccacga tgcctctaat tcttcagaat 660

ctcacagtct acccgcccaa agcattccta cgttacctgc tatttcaaaa atgaacatga 720

acgccgatgt gcctgaacaa atagaaacta aatcagagat actcacaagg cataaacaga 780

cagatgacat gccccaagtt ca 802

<210>95

<211>1412

<212>DNA

<213>Diabrotica virgifera

<400>95

gaagtgactt ctggcccgtc ttgtgcaggt tgttcagatt cgcttcgagg agaagaatcg 60

tcgctggcgg ttggattgtc gtttgcagtc gagtttgttt ttgggttctt gctctgtgaa 120

ttgtttttgt tcctcacgaa ttttggcctc cgaccatacg gataacgttg ggggctaggt 180

ttaggttttg gctgaattgc tttcttttca ggatcaaaga aatgatctaa gatatccaac 240

agctcttcca gatctttatc cttcgcattt gctacctcac tcttaataac tttttccaaa 300

ccatccttgg cgtcagaatc ataagcgatt ttaagtttgt cataattaat gttgttctct 360

gccaaaagtc tccttaaatg gctcgcgtgc tgcctctcag ttcgactggc ccgtaacagt 420

gctccaaaca ctggccggaa tgtattttgt ctttctaata ataccttaaa aagagctatt 480

tcctcttcct cagcatctat aggattgaca tatggacata ccacttccat gagatgactt 540

tctgttccag tacaatcttt actggctgcc tctcccttta attcctctct ttccccttga 600

gctaaatgag cagcaatatc aaatgaagcc cttgctctat ctactgcact gttgaagtgc 660

tctgcacccc tgtttaacaa taccttagac attatatgga gattgaaaga tttcgtggtg 720

ttgccacatt ttgcttgggc tatattgaag ccattagcaa agccttttac caccttttgg 780

aacctttcaa tcatattatg tcttcttaat gattctaaca gcatccccgg tgaggctttg 840

cgaagttcat aataaatcaa tataattcca tcaacgtcat ttcctttaac agtctccaac 900

catttcaaga agtcttggag agcagaaatt tcactcttgg tcttcaagaa ttcctgggtg 960

ttgacatctt tcaaccttct gtagcggatt gtgtttacta ctctgatacc atgtcttctg 1020

ctgtaggtag gattcaaatc tccaaatggc atgacatact gtgaaaactg tgattcaggt 1080

gtaaacgcag ctatttgaat gatttcatca attagtctgc ggccagtagt atctataccc 1140

catcctacaa gcttgtattt tccagtagga ataccctttg ctggattttc aggaacaact 1200

ggcactgaac tctccctctc atcaccagag tttgcactct ccttctgcac catcataaat 1260

tatttattat cacaaaaggc tggccaaaat aaattttata ccatcacttt tccaaaactt 1320

ataatgacaa tacaaaatta attattcata aaaaataaca attgcaatcg gaatttataa 1380

ataaaaattc aaagaaaatt ccgaaacaca cg 1412

<210>96

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>96

gcaggttgtt cagattcgct tcg 23

<210>97

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>97

ggtggtaaaa ggctttgcta atggc 25

<210>98

<211>750

<212>DNA

<213>Diabrotica virgifera

<400>98

gcaggttgtt cagattcgct tcgaggagaa gaatcgtcgc tggcggttgg attgtcgttt 60

gcagtcgagt ttgtttttgg gttcttgctc tgtgaattgt ttttgttcct cacgaatttt 120

ggcctccgac catacggata acgttggggg ctaggtttag gttttggctg aattgctttc 180

ttttcaggat caaagaaatg atctaagata tccaacagct cttccagatc tttatccttc 240

gcatttgcta cctcactctt aataactttt tccaaaccat ccttggcgtc agaatcataa 300

gcgattttaa gtttgtcata attaatgttg ttctctgcca aaagtctcct taaatggctc 360

gcgtgctgcc tctcagttcg actggcccgt aacagtgctc caaacactgg ccggaatgta 420

ttttgtcttt ctaataatac cttaaaaaga gctatttcct cttcctcagc atctatagga 480

ttgacatatg gacataccac ttccatgaga tgactttctg ttccagtaca atctttactg 540

gctgcctctc cctttaattc ctctctttcc ccttgagcta aatgagcagc aatatcaaat 600

gaagcccttg ctctatctac tgcactgttg aagtgctctg cacccctgtt taacaatacc 660

ttagacatta tatggagatt gaaagattta gtggtgttgc cacattttgc ttgggctata 720

ttgaagccat tagcaaagcc ttttaccacc 750

<210>99

<211>441

<212>DNA

<213>Diabrotica virgifera

<400>99

ggaaaagaag atcaaagtat atttaaaata gctaaaaggg attcgccgac gaagacaata 60

gtctcggatt tgaagcctgg tacaaggtat agattgtggt tggaggttta cttgactaat 120

ggaaaaatta agactagtaa cgtacaagat tttatcacca agcccagaac tgcaccgtcg 180

ttgggcgcat caacacaaca agataaactc tccagagcgg agcatcttga acccaaagga 240

gactactatg gtcctctagt cattgtagca atattagcag cgattgccat attgagtact 300

ttggtactat tactgatttt gattcgacga cataatcaaa acaaggctgc catcacacca 360

ccatctcgaa tcaatcagtc tgcttatgat aatccttcat ataaggttga aatacaacag 420

gaaacaatgg gtaaaggttt t 441

<210>100

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>100

gattcgccga cgaagacaat agtctc 26

<210>101

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>101

ctttacccat tgtttcctgt tgtatttcaa cc 32

<210>102

<211>396

<212>DNA

<213>Diabrotica virgifera

<400>102

gattcgccga cgaagacaaa gtctcggatt tgaagcctgg tacaaggtat agattgtggt 60

tggaggttta cttgactaat ggaaaaatta agactagtaa cgtacaagat tttatcacca 120

agcccagaac tgcaccgtcg ttgggcgcat caacacaaca agataaactc tccagagcgg 180

agcatcttga acccaaagga gactactatg gtcctctagt cattgtagca atattagcag 240

cgattgccat attgagtact ttggtactat tactgatttt gattcgacga cataatcaaa 300

acaaggctgc catcacacca ccatctcgaa tcaatcagtc tgcttatgat aatccttcat 360

ataaggttga aatacaacag gaaacaatgg gtaaag 396

<210>103

<211>2859

<212>DNA

<213>Diabrotica virgifera

<400>103

cgaagtgatt cagtagtacc tttaagtgat taagttttgt tagttaggga ttgtaaatta 60

agatacctac atgaaattgg ttctaatgct tgtggccgtc caggccgcgg cggtattaac 120

gtcaccgccg tcgacaccat ccacaccgtc ggcgccaccg tcgcttccaa ccaattcgaa 180

gacttgcatt gaccactgca cgtgcagctt tggaatgctg accaataggc tgcaggtaac 240

caaatgcgat aggcctctag tacttaacga cgccactttt aagtacatta gcaagacctc 300

aacgaacgtc atcacccttg acaatttgat catcaaccag atccaagtta gtgcattcgc 360

cgggttcaag aatcttgacg atgtcatcat taccaacacg agaattgcga ctatagatgt 420

gaaagccttc gagaatgtga cgcgcctaaa gttctccaaa tgtgcctttg aagacagtcc 480

tgacctcttc tctacaaagt tggaggaggt gcattttggt gaatgcaaat tagaagaaat 540

tcccaatcta aatggactga tcaatctaac gtttttaaat ttatcgggaa attacattaa 600

aaacattgaa gttgaagctt tcgctgaact gtttgatcta ggagagcttc atctttccga 660

caataacatc cacaaactcc cacctactgc gtttattaat aaccaagaat taatcaccct 720

taacctcgac aacaaccccc ttaaacattt taacattaat acatccgaca gtttagagat 780

attgtcactt aagaattgcc aattagagac atttgacgaa gaatcaacaa aaaaattaac 840

ttctcttaat gaattaaacc taagtcacaa caatcttaca gacatattca gtaaagcatt 900

gtcccacatg caggatcttt cagtaattga cctttcttac aataagttga ctaaacttca 960

agataatatt ttctctggaa acggtaaact catcaaagtt atcttagatg gaaacaattt 1020

cgacacctta ccatctttct accgccaaaa ccatgaaagt ttctctactt acacgttctc 1080

ttgtaaatct tgtaacttac gaattctttc agccaacgtc ttccagaata tggatggttt 1140

gattaatctg gaactgtcac acaataagtt aatgaatgtg gataatgtct tcgacaaaat 1200

taccagcttg aaacaattag atatttctta taatgaaatt tcatacttgt cgccagcagc 1260

ttttgtacat aacggcaatt tagaaatttt aaacattgct ggtaatcctt tactggttct 1320

taatccagaa gtgtttgccg caaatcatct tcttagagat attgacgcca gaaatgctac 1380

tctaacaaaa ttgtggtcca actacaacaa acctgttaaa tctttgcata aactgcttct 1440

aagtggaaac ttcttaggaa ctctaaccaa cgatgatttc aacatcatgc ccaaattaga 1500

ggcaatagaa ttggaagaca atcctttagt gtttgatgag aagttatgta tgttattata 1560

ctttttagat cttcaaggca ttactcctat cgattatagc aagaatatat accggagctg 1620

ataaggcttt tagggaagac attgatgatt ttgtaaccat tgaatggaaa gatgttcata 1680

aaggtaaatg tcctgaacat attactgagt ctgctttacc tgaactaact cgcatagata 1740

acaaaactac gtataaaact attgacaaaa ttgagaaaga agattatgag gaagacgatg 1800

acgacgacga tagcgattct gatgaatacg acgatgaata ctacgacgaa gaaacgacta 1860

ctaagttcca agacacatct cttgctagag ccacttacat cttgtcagta acttcagtat 1920

ttgtattaag tgctttagta gtactgacca tagctgttac cttaactcta tgcattctgc 1980

gcagaaacaa ccgctttgac atgcaacgag ctaacttacc aaggctgaaa attcctcttt 2040

ggaatacaac tccaggtcaa aagaaacaca gtgggtctgt atacagacct ctttccgaag 2100

acctctcggg tcccaagact cctaaattga gtcgctatga atttaataca accccagtag 2160

tgcatactgc ttaaagttac ttaaaattct gtggttcata gataaataag actattttgt 2220

ttaatatgta tataatcaaa ttattgtctc ttatgaaata agattagcga taaagtattc 2280

ggctgactgc agtacggcag ataacctagg gaccaaattt ccattacttc tgtgaaccaa 2340

aaccattttt aagctttaat cgtcctgggg actggagagt tcgcattttg tgcactccgc 2400

catagatagg tactgtcgaa aaatgttttg tgtattttta aaataagaca atttgaaaat 2460

tgatttttac catacccttt aaccgtttcc taacaattgc actaggtaaa gatccatttt 2520

catgtactga atttagacta ttgatttaaa atctacttaa ttttcaaatt agaagcatgc 2580

actggtatac cggtgcaatc gaactaagag ctgaatatta atgactctca tagtttgatt 2640

gcagaactat attgtcatgt cttagttcta cgtaatttaa ctgtctataa atgttattgc 2700

gcttatagta tagaaaaata ttgttaagcc acagattaag caggtattgt ttgccatatg 2760

tacctaatat cagcagcatt cagctcttct taatatctgt aaactatcta atttctagtt 2820

gtaatcaaaa tgttttcaat aaatttttta tcggaaaaa 2859

<210>104

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>104

catcttctta gagatattga cgccagaaat gc 32

<210>105

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>105

cagacccact gtgtttcttt tgacctg 27

<210>106

<211>736

<212>DNA

<213>Diabrotica virgifera

<400>106

catcttctta gagatattga cgccagaaat gctactctaa caaaattgtg gtccaactac 60

aacaaacctg ttaaatcttt gcataaactg cttctaagtg gaaacttctt aggaactcta 120

accaacgatg atttcaacat catgcccaaa ttagaggcaa tagaattgga agacaatcct 180

ttagtgtttg atgagaagtt atgtatgtta ttatactttt tagatcttca aggcattact 240

cctatcgatt atagcaagaa tatatatacc ggagctgata aggcttttag ggaagacatt 300

gatgattttg taaccattga atggaaagat gttcataaag gtaaatgtcc tgaacatatt 360

actgagtctg ctttacctga actaactcgc atagataaca aaactacgta taaaactatt 420

gacaaaattg agaaagaaga ttatgaggaa gacgatgacg acgacgatag cgattctgat 480

gaatacgacg atgaatacta cgacgaagaa acgactacta agttccaaga cacatctctt 540

gctagagcca cttacatctt gccagtaact tcagtatttg tattaagtgc tttagtagta 600

ctgaccatag ctgttacctt aactctatgc attctgcgca gaaacaaccg ctttgacatg 660

caacgagcta acttaccaag gctgaaaatt cctctttgga atacaactcc aggtcaaaag 720

aaacacagtg ggtctg 736

<210>107

<211>2859

<212>DNA

<213>Diabrotica virgifera

<400>107

cgaagtgatt cagtagtacc tttaagtgat taagttttgt tagttaggga ttgtaaatta 60

agatacctac atgaaattgg ttctaatgct tgtggccgtc caggccgcgg cggtattaac 120

gtcaccgccg tcgacaccat ccacaccgtc ggcgccaccg tcgcttccaa ccaattcgaa 180

gacttgcatt gaccactgca cgtgcagctt tggaatgctg accaataggc tgcaggtaac 240

caaatgcgat aggcctctag tacttaacga cgccactttt aagtacatta gcaagacctc 300

aacgaacgtc atcacccttg acaatttgat catcaaccag atccaagtta gtgcattcgc 360

cgggttcaag aatcttgacg atgtcatcat taccaacacg agaattgcga ctatagatgt 420

gaaagccttc gagaatgtga cgcgcctaaa gttctccaaa tgtgcctttg aagacagtcc 480

tgacctcttc tctacaaagt tggaggaggt gcattttggt gaatgcaaat tagaagaaat 540

tcccaatcta aatggactga tcaatctaac gtttttaaat ttatcgggaa attacattaa 600

aaacattgaa gttgaagctt tcgctgaact gtttgatcta ggagagcttc atctttccga 660

caataacatc cacaaactcc cacctactgc gtttattaat aaccaagaat taatcaccct 720

taacctcgac aacaaccccc ttaaacattt taacattaat acatccgaca gtttagagat 780

attgtcactt aagaattgcc aattagagac atttgacgaa gaatcaacaa aaaaattaac 840

ttctcttaat gaattaaacc taagtcacaa caatcttaca gacatattca gtaaagcatt 900

gtcccacatg caggatcttt cagtaattga cctttcttac aataagttga ctaaacttca 960

agataatatt ttctctggaa acggtaaact catcaaagtt atcttagatg gaaacaattt 1020

cgacacctta ccatctttct accgccaaaa ccatgaaagt ttctctactt acacgttctc 1080

ttgtaaatct tgtaacttac gaattctttc agccaacgtc ttccagaata tggatggttt 1140

gattaatctg gaactgtcac acaataagtt aatgaatgtg gataatgtct tcgacaaaat 1200

taccagcttg aaacaattag atatttctta taatgaaatt tcatacttgt cgccagcagc 1260

ttttgtacat aacggcaatt tagaaatttt aaacattgct ggtaatcctt tactggttct 1320

taatccagaa gtgtttgccg caaatcatct tcttagagat attgacgcca gaaatgctac 1380

tctaacaaaa ttgtggtcca actacaacaa acctgttaaa tctttgcata aactgcttct 1440

aagtggaaac ttcttaggaa ctctaaccaa cgatgatttc aacatcatgc ccaaattaga 1500

ggcaatagaa ttggaagaca atcctttagt gtttgatgag aagttatgta tgttattata 1560

ctttttagat cttcaaggca ttactcctat cgattatagc aagaatatat accggagctg 1620

ataaggcttt tagggaagac attgatgatt ttgtaaccat tgaatggaaa gatgttcata 1680

aaggtaaatg tcctgaacat attactgagt ctgctttacc tgaactaact cgcatagata 1740

acaaaactac gtataaaact attgacaaaa ttgagaaaga agattatgag gaagacgatg 1800

acgacgacga tagcgattct gatgaatacg acgatgaata ctacgacgaa gaaacgacta 1860

ctaagttcca agacacatct cttgctagag ccacttacat cttgtcagta acttcagtat 1920

ttgtattaag tgctttagta gtactgacca tagctgttac cttaactcta tgcattctgc 1980

gcagaaacaa ccgctttgac atgcaacgag ctaacttacc aaggctgaaa attcctcttt 2040

ggaatacaac tccaggtcaa aagaaacaca gtgggtctgt atacagacct ctttccgaag 2100

acctctcggg tcccaagact cctaaattga gtcgctatga atttaataca accccagtag 2160

tgcatactgc ttaaagttac ttaaaattct gtggttcata gataaataag actattttgt 2220

ttaatatgta tataatcaaa ttattgtctc ttatgaaata agattagcga taaagtattc 2280

ggctgactgc agtacggcag ataacctagg gaccaaattt ccattacttc tgtgaaccaa 2340

aaccattttt aagctttaat cgtcctgggg actggagagt tcgcattttg tgcactccgc 2400

catagatagg tactgtcgaa aaatgttttg tgtattttta aaataagaca atttgaaaat 2460

tgatttttac catacccttt aaccgtttcc taacaattgc actaggtaaa gatccatttt 2520

catgtactga atttagacta ttgatttaaa atctacttaa ttttcaaatt agaagcatgc 2580

actggtatac cggtgcaatc gaactaagag ctgaatatta atgactctca tagtttgatt 2640

gcagaactat attgtcatgt cttagttcta cgtaatttaa ctgtctataa atgttattgc 2700

gcttatagta tagaaaaata ttgttaagcc acagattaag caggtattgt ttgccatatg 2760

tacctaatat cagcagcatt cagctcttct taatatctgt aaactatcta atttctagtt 2820

gtaatcaaaa tgttttcaat aaatttttta tcggaaaaa 2859

<210>108

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>108

cgacgccact tttaagtaca ttagcaagac 30

<210>109

<211>33

<212>DNA

<213>Diabrotica virgifera

<400>109

ctaagataac tttgatgagt ttaccgtttc cag 33

<210>110

<211>740

<212>DNA

<213>Diabrotica virgifera

<400>110

cgacgccact tttaagtaca ttagcaagac ctcaacgaac gtcatcaccc ttgacaattt 60

gatcatcaac cagatccaag ttagtgcatt cgccgggttc aagaatcttg acgatgtcat 120

cattaccaac acgagaattg cgactataga tgtgaaagcc ttcgagaatg tgacgcgcct 180

aaagttctcc aaaagtgcct ttgaagacag tcctgacctc ttctctacaa agttggagga 240

ggtgcatttt ggtgaatgca aattagaaga aattcccaat ctaaatggac tgatcaatct 300

aacgttttta aatttatcgg gaaattacat taaaaacatt gaagttgaag ctttcgctga 360

actgtttgat ctaggagagc ttcatctttc cgacaataac atccacaaac tcccacctac 420

tgcgtttatt aataaccaag aattaatcac ccttaacctc gacaacaacc cccttaaaca 480

ttttaacatt aatacatccg acagtttaga gatattgtca cttaagaatt gccaattaga 540

gacatttgac gaagaatcaa caaaaaaatt aacttctctt aatgaattaa acctaagtca 600

caacaatctt acagacatat tcagtaaagc attgtcccac atgcaggatc tttcagtaat 660

tgacctttct tacaataagt tgactaaact tcaagataat attttctctg gaaacggtaa 720

actcatcaaa gttatcttag 740

<210>111

<211>464

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(313)..(407)

＜223〉n=a, c, g or t/u

<400>111

caagctaaag atagatttct ttcacaatta attagtgaaa atccatttgt agatataaat 60

atatttttga aattcaataa aattagaaaa ttaaactgta ctctggatga tttacgaaaa 120

gcaattagca agtcggatat gatagaacta tcagaatgca aagaaagaat aaagagaaag 180

atgcctataa aaattaatga aaatgtagac aattgtacaa tatatgttga aaatattaaa 240

gccgatgcta accatgagtg ggtttcacag atattttcag aatttggaaa tgtggtttat 300

gtttccatac canaatacag acataatana acaaacaaag gttttgcttt tgttgagttc 360

gaagatgaag ctggtgctaa aaatgctctt acatactttg agagtantgg gtgtaaaatg 420

ccatcagaaa caaacccgga gaaattaaag agtatcagac attc 464

<210>112

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>112

ctctggatga tttacgaaaa gcaattagca 30

<210>113

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>113

ggtttgtttc tgatggcatt ttacacc 27

<210>114

<211>336

<212>DNA

<213>Diabrotica virgifera

<400>114

ctctggatga tttacgaaaa gcaattagca agtcggatat gatagaacta tcagaatgca 60

aagaaagaat aaagagaaag atgcctataa aaattaatga aaatgtagac aattgtacaa 120

tatatgttga aaatattaaa gccgatgcta accatgagtg ggtttcacag atattttcag 180

aatttggaaa tgtggtttat gtttccatac caaaatacag acataataaa acaaacaaag 240

gttttgcttt tgttgagttc gaagatgaag ctggtgctaa aaatgctctt acatactttg 300

agagtattgg gtgtaaaatg ccatcagaaa caaacc 336

<210>115

<211>968

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(922)

＜223〉n=a, c, g or t/u

<400>115

cttttaaact tgtttagtga tctcttcatt tttaaatcgg ctgttaatga aagactgtga 60

tttttgtagt tgttgtgata tcaaaatgag gcgtactttt ggattactgc tagtttttct 120

aggattgtgg acattgtgta tagcacaaac ggaatattgt atgaccccaa ggcaggaagg 180

aggagattgc aaaataatta cacaatgtcc aagtttattt aaaattttaa aaaatcgtcc 240

tattagatct gaggacgcgg attatttaag aaaatcacaa tgtggtttcg aggggacgtt 300

accaaaagtg tgctgtgctc tgcaagaaac tgttcaaact accacgcagc gtcaagtaga 360

atatgacacc cctaaacctg cagatagtcc cctgttacca tcaaaagaag actgcggtat 420

tggaagtata ccagacaaaa tttatggagg taatacaacg gatctcgatg aatttccatg 480

gatggccctc cttgattacg aaaaagataa tggtaatcac ggattttatt gtggaggagt 540

actaattaac tcacggtata tacttacagc agctcattgt gtcaaaggaa aagatttacc 600

aagaaattgg aaactagttg gcgttaggct tggagaatat aatttagaaa cagacacaga 660

ctgtgtacaa acagtaagtg gtcaaaaatg tgcacctcct ccagttaatg ttgctgtaga 720

agaacaaatt gcccatgagc agtacaagcc ctacgatcaa aaccaatacc atgacattgc 780

cttacttaga cttgcaagaa atgtaaaaat taccggattt gttagaccca tctgtcttcc 840

aaaaactgct gatgatcaat ctagaaacta cacagggcaa aaactaacag ttgctggatg 900

gggtaaaacg gaacacgatc gnaaagtaac ataaaactca aactagatgt accaagtaaa 960

gctaatac 968

<210>116

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>116

caaggcagga aggaggagat tgc 23

<210>117

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>117

gatcatcagc agtttttgga agacagatg 29

<210>118

<211>690

<212>DNA

<213>Diabrotica virgifera

<400>118

caaggcagga aggaggagat tgcaaaataa ttacacaatg tccaagttta tttaaaattt 60

taaaaaatcg tcctattaga tctgaggacg cggattattt aagaaaatca caatgtggtt 120

tcgaggggac gttaccaaaa gtgtgctgtg ctctgcaaga aactgttcaa actaccacgc 180

agcgtcaagt agaatatgac acccctaaac ctgcagatag tcccctgtta ccatcaaaag 240

aagactgcgg tattggaagt ataccagaca aaatttatgg aggtaataca acggatctcg 300

atgaatttcc atggatggcc ctccttgatt acgaaaaaga taatggtaat cacggatttt 360

attgtggagg agtactaatt aactcacggt atatacttac agcagctcat tgtgtcaaag 420

gaaaagattt accaagaaat tggaaactag ttggcgttag gcttggagaa tataatttag 480

aaacagacac agactgtgta caaacagtaa gtggtcaaaa atgtgcacct cctccagtta 540

atgttgctgt agaagaacaa attgcccatg agcagtacaa gccctacgat caaaaccaat 600

accatgacat tgccttactt agacttgcaa gaaatgtaaa aattaccgga tttgttagac 660

ccatctgtct tccaaaaact gctgatgatc 690

<210>119

<211>542

<212>DNA

<213>Diabrotica virgifera

<400>119

cacaaaggaa acgaaagact ggaacttcat ggagaaacta ttcaaattcc aaacgtaact 60

cccgaaaatg gaggtatata cagatgcttg gccgacaatg gcttaaagga acccagtcac 120

aaacacatcg ccataaacat tgaacatgca ccaaagatct caatttccaa gtacctagta 180

acatctaaca aagaaagaga tgccgaatta gtgtgtactg tagatgccta cccagatgca 240

atggtttttt ggaaaaaagg tgaccaaatc atgaaaaacg acgagaccag gaaagttatg 300

actaagagaa gacagcacac cattgaaagc atttttgtca tcaccaatct gactgacgat 360

gacttcggta cctatacttg tgtgggcaag aatagaaatg gtaagaaaga agagaaagtg 420

agcttagtga aaactcctgc agttagggat ttcatcaaac ctgtaaatgt cagcaaagac 480

gtacttttgt cgtggaaagt tgaatcgaaa tcgcccatat cagcacatga aattcagtac 540

ag 542

<210>120

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>120

cacaaaggaa acgaaagact ggaacttc 28

<210>121

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>121

cacaaaggaa acgaaagact ggaacttc 28

<210>122

<211>542

<212>DNA

<213>Diabrotica virgifera

<400>122

cacaaaggaa acgaaagact ggaacttcat ggagaaacta ttcaaattcc aaacgtaact 60

cccgaaaatg gaggtatata cagatgcttg gccgacaatg gcttaaagga acccagtcac 120

aaacacatcg ccataaacat tgaacatgca ccaaagatct caatttccaa gtacctagta 180

acatctaaca aagaaagaga tgccgaatta gtgtgtactg tagatgccta cccagatgca 240

atggtttttt ggaaaaaagg tgaccaaatc atgaaaaacg acgagaccag gaaagttatg 300

actaagagaa gacagcacac cattgaaagc atttttgtca tcaccaatct gactgacgat 360

gacttcggta cctatacttg tgtgggcaag aatagaaatg gtaaggaaga agagaaagtg 420

agcttagtga aaactcctgc agttagggat ttcatcaaac ctgtaaatgt cagcaaagac 480

gtacttttgt cgtggaaagt tgaatcgaaa tcgcccatat cagcacatga aattcagtac 540

ag 542

<210>123

<211>2449

<212>DNA

<213>Diabrotica virgifera

<400>123

aaactcgtcc ataatcgcga tagttgagtg ggtgaggttc caagagaaac ataacatcca 60

tccacaaata tgtcgaaagt aaggatcgga gatgaagaga aggaagggca gtatggttat 120

gtccatgctg tctcaggtcc agtcgttact gctgagaaaa tgtctggttc tgctatgtac 180

gaactggtac gtgtcggata ctatgagctg gtaggagaaa tcattagatt ggaaggtgac 240

atggctacta ttcaggtata cgaagaaaca tcaggtgtaa ctgttggtga tccagtatta 300

agaactggta aaccactttc agtagaactt ggacctggta ttatgggttc catttttgat 360

ggtatccaac gtccattgaa agacatttgt gacgctactg atagtattta catccccaag 420

ggtattaacg taccttcttt atcgagaaca gcaaaatggg acttcaaccc aatcaacatc 480

aagttgggat ctcacttaac tggaggtgat atatatggtc tagttcatga aaacaccctt 540

gtcaaacaca aaatgattct gcctcctaga gctaagggta ctgtaaccta cattgcagaa 600

ccaggaaact acactgttga tgaagtagta ttggaaactg aatttgatgg tgatcgtacc 660

aaatatacta tgttgcaagt atggcctgta cgtcaagcaa ggccagtcag tgaaaaatta 720

cctgccaacc atcctctgct tacaggacag cgtgtacttg atgctctttt cccatgtgta 780

cagggtggta ctactgccat tcccggagct ttcggttgtg gaaaaactgt aatttcacaa 840

tctctttcca aatattccaa ctctgatgtc attatctacg tcggttgcgg agaaagaggt 900

aacgaaatgt ctgaagtatt gagagatttc cctgaattga ctgttgaaat tgacgggcac 960

actgaatcta ttatgaaacg taccgcattg gtcgccaaca catctaacat gcctgtagct 1020

gctcgtgaag cttctatcta tactggtatt actctttctg aatacttccg tgatatgggt 1080

tacaacgtat ctatgatggc tgactcgaca tcacgttggg ccgaagcttt gagagaaatt 1140

tcaggtcgtt tggctgaaat gcctgccgat tccggttatc cggcttactt aggtgcccgt 1200

ttggcttcct tctacgaacg tgctggtcgc gttaaatgtt taggtaatcc agacagagaa 1260

ggatccgttt caattgtagg agccgtatca cctcctggtg gtgatttctc agatcctgtt 1320

accactgcta ctcttggtat tgtacaggtg ttctggggtt tggacaagaa acttgcccaa 1380

cgtaagcact tcccttcagt agactggctt ggatcatatt ccaaatattt aagagcattg 1440

gacgactttt atgacaaaaa cttccaagag tttattcctc ttagaaccaa agttaaggaa 1500

attcttcagg aagaagatga tctagccgaa attgtgcagc tggtaggtaa agcatctctg 1560

gcagaaacgg acaaaatcac cttggaaatt gccaggcttc ttaaagaaga tttcttgcaa 1620

caaaactcat actcttctta tgacagattc tgtccattct ataaaactgt cggtatgttg 1680

agaaacatga tcggtttgta cgacatggcg agacacgctg tagaatcaac cgcacaatca 1740

gaaaataaga tcacttggaa cgtaataaga gattcaatga gtggaatttt atatcaactt 1800

agcagtatga aatttaagga tcccgtaaaa gatggtgaag ctaaaatcaa ggcagatttt 1860

gatcaattat atgaagatat tcagcaggcc ttcagaaact tagaagatta aatcttttta 1920

aggaaatttt cctattttgt tcatcagtgt aagtttaaaa atatagcgat atttatcaaa 1980

aagaataata aggcctctat ccctcacttc tgtgaatatt aatatggccg tactaaagat 2040

agtaactaaa gataggtttt ctcttttttg atattatcct gtacaaaata aattatgtaa 2100

attgttgaat atgtgtatag tttttttggg tgagggtaca gtgcttatta aatacttttt 2160

aaacattttt cccgccattc caattactat taagtttttt cgttttaata cttttttaaa 2220

tatacaggtg cttaatatcg tttatatttt cagtattact tggttttctt catgtaaatt 2280

gttttaaatt tttcttttac ccttttaatc ttgtatatta cattacccaa ttaaagttaa 2340

ttgtacagat taagataaac gagtatctta taacatctat tagattgtta gaatcaataa 2400

atgtagtgta attgttctgt tttgaacaaa taaatgcatc attattgtt 2449

<210>124

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>124

gatagttgag tgggtgaggt tccaagag 28

<210>125

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>125

caagtacacg ctgtcctgta agcagagg 28

<210>126

<211>742

<212>DNA

<213>Diabrotica virgifera

<400>126

gatagttgag tgggtgaggt tccaagagaa acataacatc catccacaaa tatgtcgaaa 60

gtaaggatcg gagatgaaga gaaggaaggg cagtatggtt atgtccatgc tgtctcaggt 120

ccagtcgtta ctgctgagaa aatgtctggt tctgctatgt acgaactggt acgtgtcgga 180

tactatgagc tggtaggaga aatcattaga ttggaaggtg acatggctac tattcaggta 240

tacgaagaaa catcaggtgt aactgttggt gatccagtat taagaactgg taaaccactt 300

tcagtagaac ttggacctgg tattatgggt tccatttttg atggtatcca acgtccattg 360

aaagacattt gtgacgctac tgatagtatt tacatcccca agggtattaa cgtaccttct 420

ttatcgagaa cagcaaaatg ggacttcaac ccaatcaaca tcaagttggg atctcactta 480

actggaggtg atatatatgg tctagttcat gaaaacaccc ttgtcaaaca caaaatgatt 540

ctgcctccta gagctaaggg tactgtaacc tacattgcag aaccaggaaa ctacactgtt 600

gatgaagtag tattggaaac tgaatttgat ggtgatcgta ccaaatatac tatgttgcaa 660

gtatggcctg tacgtcaagc aaggccagtc agtgaaaaat tacctgccaa ccatcctctg 720

cttacaggac agcgtgtact tg 742

<210>127

<211>2014

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(1772)

＜223〉n=a, c, g or t/u

<400>127

aggtattgca ggtacaccag gaaggagtat tgtcggtgct aaaggtgata aaggtgacca 60

aggaccttct ggtttagatg gtttagcagg aatacaagga gaaaagggtg atgccggaac 120

cccaggtttc cctggagtga aaggagatag gggttaccca ggtgaacaag gagcaccagg 180

aatggacggc tttccaggaa ccaatggaga aaaaggtgat agaggatttg caggagcgcc 240

cggttttaca ggagagaaag gagaccgagg tttgtcaggt cttgatggat atgaaggaca 300

aaaaggtgaa agaggccgac caggttccac cggacccttc ggcgaaccag gagaaaaagg 360

tgacgtagga gaaacaggtc ctccgggtcc gttggtgcaa attaaaggag ataaaggaga 420

accaggacct tttggattgg aaggcagaca gggtgaaaaa ggagaccgtg gattctctgg 480

agctattggt ctacgaggag agaaagggga tgtaggtttc cctggtgtta aaggaaatct 540

tggtagccca gggcgtccag gagctaaagg tgaacgaggt ctttctgggg tagctggatt 600

accagggttg acaatcaagg gggagaaagg tttgcctgga atacctggca aaaacggaag 660

agaaggtata cctggcaccc cagggcaaat tggtgaacca ggattgccag gtttggacgg 720

cagacaagga ccacctggtc caccaggtcc actgggtctt caagggcaga agggagatcg 780

aggttttgat ggagctccag gaccacaagg gccaaccggg cccccaggac agccaggatt 840

agaaggcgcc cctggacaac gaggtgataa gggagaaaga ggtaaccccg gtgctttcgg 900

agctccggga caaaagggag atcgtggaca aacaggattg caaggaatct caggctatcc 960

gggagaaaaa ggagacagag gttacgatgg aacgcctgga attgaaggac caatgggacc 1020

acctggtctt aaaggagatg ctggtattcc aggacaatct ggaagaccgg gtattccagg 1080

tcaaaagggt gacaaaggag agcgtgcact tcaaggaggt ccaggtccaa aaggagatag 1140

aggttttcct ggaaatcctg ggttaccagg agagagggga cctgcaggtc tcgacggaat 1200

accaggagtt cacggacaaa agggagacaa aggtgagaga ggattcactg gtattgccgg 1260

atcgcctgga ttacaaggag aaaaaggtga tataggtatc gaaggagcaa taggacttca 1320

aggttctatc ggtgatatcg gtccaaaagg tgaacctggt gcttcttgcg ttgatgtccc 1380

tgattatctc actggttctt tactggttag acacagtcaa agtaaagaag ttccaagctg 1440

cgaacgtggt catatccaat tgtgggaagg ttattcctta ctatacgttg agggcaatga 1500

aaaggctcat actcaagact taggttacgc tggctcctgt ataaggaagt tctcaacgat 1560

gccatttgtg ttctgtgatg ttaataacgt ctgtaactac gcaagtagaa acgataaatc 1620

ctactggctc tccaccagtg cagctatacc aatgatgccc gtagaggaat cgcagattga 1680

ggagtatatt tcaagatgtg tcgtatgtga agcacctgcc agtgtcattg ccgtgcacag 1740

tcaatctcta gcacttccag aatgcccata cnggatggag ttccttatgg atcggctaca 1800

gttttgtaat gcacactgca gccggagccg aaggtggagg tcaatctctt tccagtcccg 1860

gttcatgttt agaggacttc agggccacac cgttcatcga atgtaacggt gctcaaggat 1920

catgtcacta tttcgccaac acccttagtt tctggctcgc gaccatcgac aacaaccaac 1980

aattccagaa acctcagaaa cagacactca aagc 2014

<210>128

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>128

gtgaacaagg agcaccagga atgg 24

<210>129

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>129

ccaggcgttc catcgtaacc tctg 24

<210>130

<211>838

<212>DNA

<213>Diabrotica virgifera

<400>130

ccaggcgttc catcgtaacc tctgtctcct ttttctcccg gatagcctga gattccttgc 60

aatcctgttt gtccacgatc tcccttttgt cccggagctc cgaaagcacc ggggttacct 120

ctttctccct tatcacctcg ttgtccaggg gcgccttcta atcctggctg tcctgggggc 180

ccggttggcc cttgtggtcc tggagctcca tcaaaacctc gatctccctt ctgcccttga 240

agacccagtg gacctggtgg accaggtggt ccttgtctgc cgtccaaacc tggcaatcct 300

ggttcaccaa tttgccctgg ggtgccaggt ataccttctc ttccgttttt gccaggtatt 360

ccaggcaaac ctttctcccc cttgattgtc aaccctggta atccagctac cccagaaaga 420

cctcgttcac ctttagctcc tggacgccct gggctaccaa gatttccttt aacaccaggg 480

aaacctacat cccctttctc tcctcgtaga ccaatagctc cagagaatcc acggtctcct 540

ttttcaccct gtctgccttc caatccaaaa ggtcccggtt ctcctttatc tcctttaatt 600

tgcaccaacg gacccggagg acctgtttct cctacgtcac ctttttctcc tggttcgccg 660

aagggtccgg tggaacctgg tcggcctctt tcaccttttt gtccttcata tccatcaaga 720

cctgacaaac ctcggtctcc tttctctcct gtaaaaccgg gcgctcctgc aaatcctcta 780

tcaccttttt ctccattggt tcctggaaaa ccgtccattc ctggtgctcc ttgttcac 838

<210>131

<211>771

<212>DNA

<213>Diabrotica virgifera

<400>131

ggcaatgatt tgtctgcgaa aatttttgca accatggaaa aacataaaga agtgttcttt 60

gtcattaggt tacattctgt acagtctgct gctagcttaa agccaattca ggatcctgat 120

ccttttataa actgtgattt aatggatggt cgagatgcat tcctaacact cgcccgtgaa 180

aaacattatg aattctcttc tttaagaagg gccaaatata gtactttgtc tatgttatat 240

gaattgcata atcaaggtca agataaattt gtatatactt gtaatagttg taaagcccat 300

gtggaaacaa ggtatcattg tactgtgtgt gaagactttg acttgtgcat ccaatgttat 360

gagaaagaag gtcatcctca caaaatggag aaacttggat tggatattga tgatggaagt 420

tcacctagtg atagcaaagc caatccagga gaagctagaa aattatctat acagcgttgt 480

atccattctt tggtacatgc ttgtcaatgc agagatgcca attgtcggtt accaagctgt 540

cagaagatga aacgagtcac aaatcacacc aaattgtgca agaggaaaac aaatggtggt 600

tgccctattt gcaaacaact catcgctcta tgttgctatc atgccaaaca ctgcccagaa 660

aacaagtgtc ctgtaccatt ctgttcatgc ataaagcaca aactaaaaca acaacaattg 720

caacaaaggt tgcaacaggt gcaactactt agtataagaa tggtagttat g 771

<210>132

<211>22

<212>DNA

<213>Diabrotica virgifera

<400>132

gcattcctaa cactcgcccg tg 22

<210>133

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>133

ggacacttgt tttctgggca gtgtttg 27

<210>134

<211>515

<212>DNA

<213>Diabrotica virgifera

<400>134

gcattcctaa cactcgcccg tgaaaaacat tatgaattct cttctttaag aagggccaaa 60

tatagtactt tgtctatgtt atataaattg cataatcaag gtcaagataa atttgtatat 120

acttgtaata gttgtaaagc ccatgtggaa acaaggtatc attgtactgt gtgtgaagac 180

tttgacttgt gcatccaatg ttatgagaaa gaaggtcatc ctcacaaaat ggagaaactt 240

ggattggata ttgatgatgg aagttcacct agtgatagca aagccaatcc aggagaagct 300

agaaaattat ctatacagcg ttgtatccat tctttggtac atgcttgtca atgcagagat 360

gccaattgtc ggttaccaag ctgtcagaag atgaaacgag tcacaaatca caccaaattg 420

tgcaagagga aaacaaatgg tggttgccct atttgcaaac aactcatcgc tctatgttgc 480

tatcatgcca aacactgccc agaaaacaag tgtcc 515

<210>135

<211>1120

<212>DNA

<213>Diabrotica virgifera

<400>135

atttagccca aaatgtctga agaaagtgaa aaaaactcgt tggaaaaagt tagtgaagtc 60

gatggaagta cggacggaat aatattaaat aaaagtgcaa agaaacgtaa caaaaataaa 120

aagaaaaaga atgaaaacca agaagaaaac agtgaccaaa gcaacgaggt tgtccaagcc 180

gaaaacaact tattatctaa cttatcgaac cctattaata caatatcaat taaggagcta 240

aaagcagcca tggaggtatt tactctatca caaaaacctg ccaagagttc agaggaagct 300

ctaaagaaga cattccagtt ttggaatacc cagcctgttc ccaaaatgga cgaaaaaata 360

actgttaacg aagccattga accagacaaa gaacctgctg acattagaca agaaccgtac 420

agtttgcctg atggttttat gtgggacact cttaatctag atgaaccgtt ggtgctcaaa 480

gagttatata cgttactcaa tgaaaactat gttgaagatg acgattgtat gtttagattt 540

gattatcaac ctgaattttt gaaatgggct ttgcaacccc cagggtggaa gcgagaatgg 600

cattgtggag tcagagttgt aaaaagtggt agattagtgg gatttatctc tgcaattcca 660

gcaactctaa acatttataa aaagacacaa agaatggtgg agataaattt tctatgtgta 720

cataaaaagc tacgttccaa acgagtcgca ccagtgttga ttcgagaaat cacccgcaga 780

gtacacctga acggcctatt tcaagcagtg tacaccgccg gcatagtcct accgaaacca 840

gtgagtactt gtacctactg gcatcgatcg ctgaatccga aaaaattaat cgaggtgaag 900

ttttctcatt tgtctcgtaa tatgacgctg caacggactc tcaagttgta caaactgccg 960

gacaagccta agactccggg atttagaaaa atgacagccg aagatatacc tcaagctcac 1020

aaattactag tagagtattt aagtcgattc gacctagcgc catattttaa cgaagaagag 1080

ttcacacatt ggttcctacc tagaacgaac ataatcgaca 1120

<210>136

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>136

gtgaccaaag caacgaggtt gtcc 24

<210>137

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>137

gtaggtacaa gtactcactg gtttcggtag g 31

<210>138

<211>707

<212>DNA

<213>Diabrotica virgifera

<400>138

gtgaccaaag caacgaggtt gtccaagccg aaaacaactt attatctaac ttatcgaacc 60

ctattaatac aatatcaatt aaggagctaa aagcagccat ggaggtattt actctatcac 120

aaaaacctgc caagagttca gaggaagctc taaagaagac attccagttt tggaataccc 180

agcctgttcc caaaatggac gaaaaaataa ctgttaacga agccattgaa ccagacaaag 240

aacctgctga cattagacaa gaaccgtaca gtttgcctga tggttttatg tgggacactc 300

ttaatctaga tgaaccgttg gtgctcaaag agttatatac gttactcaat gaaaactatg 360

ttgaagatga cgattgtatg tttagatttg attatcaacc tgaatttttg aaatgggctt 420

tgcaaccccc agggtggaag cgagaatggc attgtggagt ccgagttgta aaaagtggta 480

gattagtggg atttatctct gcaattccag caactctaaa catttataaa aagacacaaa 540

gaatggtgga gataaatttt ctatgtgtac ataaaaagct acgttccaaa cgagtcgcac 600

cagtgttgat tcgagaaatc acccgcagag tacacctgaa cggcctcttt caagcagtgt 660

acaccgccgg tatagtccta ccgaaaccag tgagtacttg tacctac 707

<210>139

<211>940

<212>DNA

<213>Diabrotica virgifera

<400>139

cgtcaggttg gtcaattcag tattcagaat agtagaattt tgaattttct gtacgtgtac 60

gtgagtcgtg agtgaatcgt atcgtatcgt ggtatcgtat ccggtccggt tagctaagaa 120

tgtgtcaaac agggaagttt ttaaattgtt aatttagaag ttaatttttt gattaagtta 180

tacctaagtg cgaaattttc aaaaatgggc caacacgtat ctagaacaga tttcgaatgg 240

gtatataccg aggaacctca cgctagccgc aggaagcaaa tcttagaaaa atatccccaa 300

atcaagaaac tctttggata cgatccgaac ttcaaatggg tcgtaacgag catggtactt 360

acacaattcg tacttctgta cttactcaaa gataggtcat ggccagtgct aattttagta 420

gcgtattgct taggaggtgt catcaatcac tcgttaatgt tggctataca tgaaatttcc 480

cacaatttgg cgtttggaca cgcacggccg ttgcacaata aattatttgg attttttgcc 540

aatttgccaa taggattacc gattactata agctttaaaa aataccactt ggaacaccac 600

agatatcaag gtgacgaaca gaaagatacg gacatcccaa cgtacataga ggccaaacta 660

ttttgcacga cctttggaaa attcatttgg gtgatactgc aaccgttttt ctactctttc 720

cgcccactcg tagtttatcc gaagccgcct acgcttttgg aattcgtaaa cctggtaatt 780

caactcgcct tcgatgctct cgttgtttat tttttcggag gaagaatgtt gggatatctc 840

cttattggat ctgtcatggc tatgggtctt catccggtag cgggacactt catcagtgaa 900

cattacatgt tcaaaaaggg ctacgaaacg tacagctact 940

<210>140

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>140

gtcaggttgg tcaattcagt attcagaata gtag 34

<210>141

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>141

ggataaacta cgagtgggcg gaaagag 27

<210>142

<211>746

<212>DNA

<213>Diabrotica virgifera

<400>142

gtcaggttgg tcaattcagt attcagaata gtagaatttt gaattttctg tacgtgtacg 60

tgagtcgtga gtcgtgagtg aatcgtatcg tatcgtggta tcgtatccgg tccggttagc 120

taagaatgtg tcaaacaggg aagtttttaa attgttaatt tagaagttaa ttttttgatt 180

aagttatacc taagtgcgaa attttcaaaa atgggccaac acgtatctag aacagatttc 240

gaatgggtat ataccgagga acctcacgct agccgcagga agcaaatctt agaaaaatat 300

ccccaaatca agaaactctt tggatacgat ccgaacttca aatgggtcgt aacgagcatg 360

gtacttacac aattcgtact tctgtactta ctcaaagata ggtcatggcc agtgctaatt 420

ttagtagcgt attgcttagg aggtgtcatc aatcactcgt taatgttggc tatacatgaa 480

atttcccaca atttggcgtt tggacacgca cggccgttgc acaataaatt atttggattt 540

tttgccaatt tgccaatagg attaccgatt actataagct ttaaaaaata ccacttggaa 600

caccacagat atcaaggtga cgaacagaaa gatacggaca tcccaacgta catagaggcc 660

aaactatttt gcacgacctt tggaaaattc atttgggtga tactgcaacc gtttttctac 720

tctttccgcc cactcgtagt ttatcc 746

<210>143

<211>1049

<212>DNA

<213>Diabrotica virgifera

<400>143

gtataaatat ttataaaata caatggctaa gtttcactta agaaaaaagt ctaaacgaca 60

gccagcgcgt ctcagatata agattgaaaa gaaagtccga gagcacaaca gaaaactcaa 120

gaaagaaaat aagaacaacc ctaaaaagaa aaaggaaata atgcaaatcc caaacatatg 180

tccttttaaa gaggacattc ttaaagaagt agaagcacta aagaaacaaa aggaagaaga 240

aaaacttaag ctaagagaag ctgcaaaatt ggaaaggcaa aaggagaaag aggaaagtaa 300

aaaagagaaa cttactgcag gacttcaggg attagtgcaa aatgctgaac taaggagtaa 360

acttcatgaa catttaacac cagataataa tgtaaaacag cacgagacaa ataatgagca 420

gtccctgaaa caatattaca aagagtttaa gaaggttatc gaagctgcag atgttattct 480

agaagtagtt gacgctagag atcctttagg gactagatgt aagcaagtag agcaggctgt 540

gttagataca aaaggtaata aaagactagt cttagtatta aacaaggcag acttagttcc 600

tagagaaatt ttggatcaat ggctaaagta cttgaagaaa accactccca ctatagcatt 660

caaagcatca actcaaaatc aaagtaaaaa gttaggacag agaaagcttg gaaagggaaa 720

taaggtattg cagagttcaa attctgtagg agcagaattg ttaatgtctc tgctagcaaa 780

ctattgtagg aacaagggta tcaaaacttc tataagagtt ggaattgtag gtttgcctaa 840

tgtaggaaaa agttcaatta ttaacagctt aaaaaggtct agagcttgta atgttggtgc 900

aacacctggt gttactaggg caatgcagga agtacaatta gactcaaaga taaaactttt 960

ggattctcct ggtatagttt ttgcatctgg aaatgacagt agtgcttctt tgagaaatgc 1020

agttcgaatt tctgccttga gtgatcctg 1049

<210>144

<211>35

<212>DNA

<213>Diabrotica virgifera

<400>144

gtctcagata taagattgaa aagaaagtcc gagag 35

<210>145

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>145

gctttgaatg ctatagtggg agtggttttc 30

<210>146

<211>598

<212>DNA

<213>Diabrotica virgifera

<400>146

ttctcagata taagattgaa aagaaagtcc gagagcacaa cagaaaactc aagaaagaaa 60

ataagaacaa ccctaaaaag aaaaaggaaa taatacaaat cccaaacata tgtcctttta 120

aagaggacat tcttaaagaa gtagaagcac taaagaaaca aaaggaagaa gaaaaactta 180

agctaagaga agctgcaaaa ttggaaaggc aaaaggagaa agaggaaagt aaaaaagaga 240

aacttactgc agaacttcag ggattagtgc aaaatgctga actaaggagt aaacttcatg 300

aacatctaac accagataat aatgtaaaac agcacgagac aaataatgag cagtccctga 360

aacaatatta caaagagttt aagaaggtta tcgaagctgc agatgttatt ctagaagtag 420

ttgacgctag agatccttta gggactagat gtaagcaagt agagcaggct gtgttagata 480

caaaaggtaa taaaagacta gtcttagtat taaacaaggc agacttagtt cctagggaaa 540

ttttggatca atggctaaag tacttgaaga aaaccactcc cactatagca ttcaaagc 598

<210>147

<211>889

<212>DNA

<213>Diabrotica virgifera

<400>147

gtaaaatagt ttcaaaatgc atcaggaggc catacaactc atccaggtgc tagaaaaaac 60

agtttcaccg gataaaaatg aattggaaca agcgtcatcg tttctcgaac aagctgcagt 120

gactaaccta ctagaattca tcaaaactct atcagatata ttaagacatg gcggaaatag 180

ccctgtggcc cgaatggccg caggtttgca gttaaaaaat caactcacct ctaaagactc 240

caacataaag accacctacc aacaacggtg gttggctttc ccagaggacg ttcgggggta 300

tattaaaaat aacgttgtgt gcgcacttgg aaccgaaaca agcagaccat catccgcagc 360

tcagtgcgta gcatacgtcg cagtaacaga actaccacaa gggcaatggc cagatctcat 420

agcaacgttg gtcaacaatg tttgccatgc aaattccaca gaaatgcaaa aggaagccac 480

attagaagct atcggttata tatgccaaga aatcgactcg gacgtattag tatctcaacc 540

aaacgacatc ttaacagcta tagttcatgg aatgcgagcc tcggaaccga gcaaccacgt 600

tcgtttagca gctacacagg ccctattaaa ctctctagaa ttcacaaagg ccaactttga 660

ccaaccgacg gaacggaact atatcatgga agtcgtctgt gaagccacgc agtctccaga 720

ttcacagatc agagtggccg ctttgcagtg tctagttaag attatgtctt tgtattatca 780

acacatggaa ccttatatgg ctcctgctct attccctatc actctagaag ccatgaagtc 840

ggaaaaacga tgctgtttct ctacagggta tcgaattctg gtctaacgt 889

<210>148

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>148

gccatacaac tcatccaggt gctaga 26

<210>149

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>149

gtgataggga atagagcagg agccatataa g 31

<210>150

<211>792

<212>DNA

<213>Diabrotica virgifera

<400>150

gccatacaat tcatccagtg ctagaaaaaa cagtttcacc ggataaaaat gaatggaaca 60

agcgtcatcg tttctcgaac aagctgcagt gactaaccta ctagaattca tcaaaactct 120

atcagatata ttaagacatg gcggaaatag ccctgtggcc cgaatggccg caggtttgca 180

gttaaaaaat caactcacct ctaaagactc caacataaag accacctacc aacaacggtg 240

gttggctttc ccagaggacg ttcgggggta tattaaaaat aacgttgtgt gcgcacttgg 300

aaccgaaaca agcagaccat catccgcagc tcagtgcgta gcatacgtcg cagtaacaga 360

actaccacaa gggtaatggc cagatctcat agcaacgttg gtcaacaatg tttgccatgc 420

aaattccaca gaaatgcaaa aggaagccac attagaagct atcggttata tatgccaaga 480

aatcgactcg gacgtattag tatctcaatc aaacgacatc ttaacagcta tagttcatgg 540

aatgcgagcc tcggaaccga gcaaccacgt tcgtttagca gctacacagg ccctattaaa 600

ctctctagaa ttcacaaagg ccaactttga ccaaccgacg gaacggaact atatcatgga 660

agtcgtctgt gaagccacgc agtctccaga ttcacagatc agagtggccg ctttgcagtg 720

tctagttaag attatgtctt tgtattatca acacatggaa ccttatatgg ctcctgctct 780

attccctatc ac 792

<210>151

<211>685

<212>DNA

<213>Diabrotica virgifera

<400>151

tccccttccc cgcgtccgca gcatatgcta tacggatcat gccgaagcga ttcaaccagg 60

ctgatagagc tataaatatg gcctgcaaga ctggaagatg ggtcatgctg aagaacgtac 120

acttggctcc tcaatggtta gtccagctgg agaagaagct tcattcactt caacctcacg 180

ccaactttag actattcctc accatggaaa ttaatccgaa actaccagtt aatcttctta 240

gagcaggaag aatatttgta ttcgaacctc caccgggtat tagagctaac ctcttgagaa 300

cattcaacac agttccagtc agtaggatga tgaaagcacc aaacgaaaca gcaagacttt 360

acttcttatt ggcgtggttc cacgcgattg tccaggagag gttgatatat tgccctctag 420

gttgagcaaa acattatgaa tttagtgaat catatttaag agtcgcctgc gatactttgg 480

atacttggat tgataccacg gctatgggaa tgactaactt gccggcagag aaactctcat 540

gtgacaccct agccactctg ctctcgcaat ccatctatgg tggtaaaatc gataacgatt 600

ccgatcaacg actgttacat tccttcctga atctgctttt tactcccata agctttgaaa 660

gcgactttcg cttcaagtcc aacat 685

<210>152

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>152

ggatcatgcc gaagcgattc aac 23

<210>153

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>153

gaatgtaaca gtcgttgatc ggaatcg 27

<210>154

<211>588

<212>DNA

<213>Diabrotica virgifera

<400>154

gatcatgccg aagcgattca accaggctga tagagctata aatatggcct gcaagactgg 60

aagatgggtc atgctgaaga acgtacactt ggctcctcaa tggttagtcc agctggagaa 120

gaagcttcat tcacttcaac ctcacgccaa ctttagacta ttcctcacca tggaaattaa 180

tccgaaacta ccagttaatc ttcttagagc aggaagaata tttgtattcg aacctccacc 240

gggtattaga gctaacctct tgagaacatt cagcacagtt ccagccagta ggatgatgaa 300

agcaccaaac gaaagagcaa gactttactt cttattggcg tggttccacg cgattgtcca 360

ggagaggttg agatattgcc ctctaggttg ggcaaaacat tatgaattta gtgaatcaga 420

tttaagagtt gcctgtgata ctttggatac ttggattgat acaacggcta tgggaaggac 480

gaacttgccg ccagagaaag tcccatggga cgccctagtc actctgctct cgcaatccat 540

ctatggtggt aaaatcgata acgattccga tcaacgactg ttacattc 588

<210>155

<211>946

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(802)..(849)

＜223〉n=a, c, g or t/u

<400>155

atccgaacat cctgacttgg taaaattaag atctgtggga aaatctgtaa gaaataggga 60

tttgtgggct ttagaaataa attctaatgt ccgaaacagg acattgttaa caccaatgtt 120

taaatatgtt gctaatatgc atggggatga agccatagga agacagttga tgatttacct 180

ggctctctat ttaattcaca attatggcac taatgatagg gtgaccagat tggttaattc 240

aactgatatt tatttaatgc cttcaatgaa ccctgatggt tatgaaaatt cagaagaggg 300

cagatgtgaa tctaaaaaac aatatgttgg aagagaaaat gaaaatagtg ttgatttaaa 360

tagagatttt ccagatcagt ttgatgctac aaaagcagga acaattctgt ctggtaggca 420

gcctgaaacg atagctatga tgacttggat tatttccaga ccatttgttt tatctggaaa 480

tttgcacgga ggtgcagtag tagctagcta tccgtatgat gattctaact ctggtcgaag 540

atgctgtgta gagagtagat caccagataa taatattttc aaaacgttag cattaaacta 600

cgctcaatcg aatcctgtga tgaaaaaggg taatgcttgt aaagacgata catttgatca 660

gggcatcaca aatggtgctt tttggtatga agtgagaggt ggcatgcaag acttcaacta 720

cgttcactcc aactgtttcg aagtagcttt tgaactgagt tgctgtaaat ttccgttagc 780

caaaactcta cctcaggaat gngcaacaac aaggaagccc tactaacttt catggagtca 840

acacattgng gcgctaaggg attggttaga aacgaaaacg gtgacgccgt tttggacgcc 900

gacgtagtgg ttgtgggtat tacacataac gtcactacat ccaacc 946

<210>156

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>156

ctgtaagaaa tagggatttg tgggctttag 30

<210>157

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>157

gttggagtga acgtagttga agtcttgc 28

<210>158

<211>689

<212>DNA

<213>Diabrotica virgifera

<400>158

ctgtaagaaa tagggatttg tgggctttag aaataaattc taatgtccaa aacaggacat 60

tgttaacacc aatgtttaaa tatgttgcta atatgcatgg ggatgaagcc ataggaagac 120

agttgatgat ttacctggct ctctatttaa ttcacaatta tggcactaat gatagggtga 180

ccagattggt taattcaact gatatttatt taatgccttc aatgaaccct gatggttatg 240

aaaattcaga agagggcaga tgtgaatcta aagaacaata tgttggaaga gaaaatgaaa 300

atagtgttga tttaggtaga gattttccag atcagtttga tgctacaaaa gcaggaacaa 360

ttctgtctgg taggcagcct gaaacgatag ctatgatgac ttggattatt tccagaccat 420

ttgttttatc tggaaatttg cacggaggtg cagtagtagc tagctatccg tatgatgatt 480

ctaactctgg tcgaagatgc tgtgtagaga gtagatcacc agataataat attttcaaaa 540

cgttagcatt aaactacgct caatcgaatc ctgtgatgaa aaagggtaat gcttgtaaag 600

acgatacatt tgatcagggc atcacaaatg gtgctttttg gtatgaagtg agaggtggca 660

tgcaagactt caactacgtt cactccgac 689

<210>159

<211>658

<212>DNA

<213>Diabrotica virgifera

<400>159

gccgtggact tagcgggctg cgggatgatc gtgatctcgt cattgatctt cagaggtttc 60

aactgatctg cagccgacgg cttgggagag ctgttggaag ttgtcgaaga ggtcgtggag 120

ccgcccttct tgtccatgac tcgcttggcg acgacgctgt gggcggaata gacgtggttc 180

tcgaattgtt tgtacattcc gaaggtggcc gtgcacactg tgcatttgta ggacaggtgc 240

gcgggtttca acgtcatgtt atgatcccgg gccacgtggt ttagtaattt ccattggtag 300

actcttccac aaacaggaca ccttcctgaa tctttgctct tgttggcagc atcctgcatg 360

ctggaggtga ctaatccatg agagcctaat aaatgccttt ccaacccttg atctgtgaag 420

aacctaaatt gacacttttg acaatttaat ggcggcctgt tatatatcat cttgggatga 480

atcttaatct tgtgtatcca ttgcatgtga tttctaagct gttcaaggtc cttgatgtac 540

ccatcgcaga tctcgcagat gacgaacgag ttcttggagg atgtatcccc tggtttggcg 600

ccgcccgaag atccaggtgc ggctgcggca gcagatgggg actagttcta gatcgcga 658

<210>160

<211>21

<212>DNA

<213>Diabrotica virgifera

<400>160

gacttagcgg gctgcgggat g 21

<210>161

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>161

gatacatcct ccaagaactc gttcgtca 28

<210>162

<211>580

<212>DNA

<213>Diabrotica virgifera

<400>162

gacttagcgg gctgcgggat gatcgtgatc tcgtcattga tcttcagagg tttcaactga 60

tctgcagccg acggcttggg agagctgttg gaagttgtcg aagaggtcgt ggagccgcct 120

ttcttgccat gactcgcttg gcgacgacgc tgtgggcgga atagacgtgg ttctcgaatt 180

gtttgtacat gccgaatgtg gccgtgcaca ctgtgcattt gtaggacagg tgtgcgggtt 240

tcaacgtcat gttatgatcc cgggccacgt ggtttagtaa tttccattgg tagactcttc 300

cacaaacagg acaccttcct gaatctttgc tcttgttggc agcatcctgc atgctggagg 360

tgactaatcc atgagagcct aataaatgcc tttccaaccc ttgatctgtg aagaacctaa 420

attgacactt ttgacaattt aatggcggcc tgttatatat catcttggga tgaatcttaa 480

tcttgtgtat ccattgcatg tgatttctaa gctgttcgag gtccttgatg tacccatcgc 540

agatctcgca gatgacgaac gagttcttgg aggatgtatc 580

<210>163

<211>888

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(771)

＜223〉n=a, c, g or t/u

<400>163

cttattttat ggtgtcgtgc cagcagaaaa ttttaaaaat tcttgtaatt gtttagacat 60

ttcgcaacaa ttgtgaacaa ttttggaaaa tattgcttga aagctataat tctagatgta 120

agagcaaagt tttgtagtca aaatggctgg agtattcgat ttagaactgc atgatgttga 180

aactgtagaa gtagatgatt cagacgatgc aatcgaaatt gaagaggatg aatatgatca 240

aaatcctaat attaatgcaa ttgtagaatc cgatgataca gaaacggttc atctgactga 300

acaaattgtg aatccgggcc aggaaaaaac cggaccccat gactttgaat tacgcaaagt 360

cctgggcaaa ggaggttatg gcaaagtatt tcaagttaga aaaattacgg ggaaagatgc 420

caatactgtc tttgccatga aggttctccg caaggcttcc atagttagaa atcaaaaaga 480

tactgcccat acgaaagcag aacgaaatat tttagaagaa gttaagcatc cttttatagt 540

ggatttgata tatgccttcc aaacgggtgg aaaattgtac ttaattttag aatacctcag 600

cgggggcgaa ctttttatgc atttagaaag agaaggaata tttttagagg ataccgcttg 660

cttttattta tccgaaatca ttctcgccct agaacattta catggacaag gaattattta 720

tagagatcta aagcctgaaa atattttact tgacgcccac ggtcacgtta nattgacaga 780

tttcggtctg tgtaaaaaca cattaacgaa ggcatattga cccacaccgt tttgtggaac 840

tattgaatat atggcacctg aaatttaact agacatggcc atggaaaa 888

<210>164

<211>33

<212>DNA

<213>Diabrotica virgifera

<400>164

gcatgatgtt gaaactgtag aagtagatga ttc 33

<210>165

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>165

gtgtgggtca atatgccttc gttaatg 27

<210>166

<211>658

<212>DNA

<213>Diabrotica virgifera

<400>166

cttatgttga aactgtagaa gtagatgatt cagacgatgc aatcgaaatt gaagaggatg 60

aatatgatca aaatcctaat attaatgcaa ttgtagaatc cgatgataca gaaacggttc 120

atctgactga acaaattgtg aacccgggcc aggaaaaaac cggaccccat gactttgaat 180

tacgcaaagt cctgggcaaa ggaggttatg gcaaagtatt tcaagttaga aaaattacgg 240

ggaaagatgc caatactgtc tttgccatga aggttctccg caaggcttcc atagttagaa 300

atcaaaaaga tactgcccat acgaaagcag aacgaaatat tttagaagaa gttaagcatc 360

cttttatagt ggatttgata tatgccttcc aaacgggtgg aaaattgtac ttaattttag 420

aatacctcag cgggggcgaa ctttttatgc atttagaaag agaaggaata tttttagagg 480

ataccgcttg cttttattta tccgaaatca ttctcgccct agaacattta catggacaag 540

gaattattta tagagatcta aagcctgaaa atattttact tgacgcccac ggtcacgtta 600

aattgacaga tttcggtctg tgtaaagaac acattaacga aggcatattg acccacac 658

<210>167

<211>886

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(805)

＜223〉n=a, c, g or t/u

<400>167

cgtttactaa acgtaaacga ataaaacaat aaaagtacaa tgaaaattat ttaaataaga 60

acataagtat ttcagtatgg atcgaccaag tacaagtaag cgaaatgtct ttgactccga 120

ttcagatagt gtcgacagca ctgaagaagc tcgccgaaga gatctgaagg aaagagatga 180

attcgcaaat aggctcaaaa aacgcgatga agaacgtact agaaacgttg ccagtacttc 240

tgataagcgc gcttatgaag aggctgctaa aagacttaaa ttggacaatg cagaaaaaga 300

gaaacttatt cctgcactca gagttgcttc caggaggaag tatttggaga agagaaagga 360

ggacaaacta caagaattgg aagctgatat tgccgatgat gagtaccttt ttgaggaaga 420

tgttttgact gaaagagaga aaaaagatcg tgtacacaag aaagagctgt taaaattggc 480

gcaggaacat gaaaaggcca gggaacttga gcgggttcaa aggtatcaca tgcccagaga 540

tttgggcaag gactccactg ctgattacat tgaagttgat gacttggaga aggtaccaca 600

atctgaacag aagaaatggg aaaaagatca gatggcctcg gcagtgttta agtttggagc 660

aaaagatggg attaaaaaag aagagtatga gttagtgtta gaagatcaaa ttcagtttat 720

acaagcacta caaatgccag gagtgtctga taagaaagaa cccgaactaa cggaaacaga 780

aaagaaaaaa cttactatag aaganacaaa gaaaagcctt ccgatatacc cttttaaaga 840

cgacttaata caggccatta gggaacacca agttttgatt atcgaa 886

<210>168

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>168

cgaccaagta caagtaagcg aaatgtcttt g 31

<210>169

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>169

gttctttctt atcagacact cctggcattt g 31

<210>170

<211>679

<212>DNA

<213>Diabrotica virgifera

<400>170

cgaccaagta caagtaagcg aaatgtcttt gactccgatt cagatagtgt cgacagcact 60

gaagaagctc gccgaagaga tctgaaggaa agagatgaat tcgcaaatag gctcaaaaaa 120

cgcgatgaag aacgtactag aaacgttgcc agtacttctg ataagcgcgc ttatgaagag 180

gctgctaaaa gacttaaatt ggacaatgca gaaaaagaga aacttattcc tgcactcaga 240

gttgcttcca ggaggaagta tttggagaag agaaaggagg acaaactaca agaattggaa 300

gctgatattg ccgatgatga gtaccttttt gaggaagatg ttttgactga aagagagaaa 360

aaagatcgtg tacacaagaa agagctgtta aaattggcgc aggaacatga aaaggccagg 420

gaacttgagc gggttcaaag gtatcacatg cccagagatt tgggcaagga ctccactgct 480

gattacattg aagttgatga cttggagaag gtaccacaat ctgaacagaa gaaatgggaa 540

aaagatcaga tggcctcggc agtgtttaag tttggagcaa aagatgggat taaaaaagaa 600

gagtatgagt tagtgttaga agatcaaatt cagtttatac aagcactaca aatgccagga 660

gtgtctgata agaaagaac 679

<210>171

<211>910

<212>DNA

<213>Diabrotica virgifera

<400>171

accaaaatta taacccagga aggatattga aaatattcag aacttttagg aacccagaag 60

gacgcgaatt cacccgcgta gaatacgtcc ggaagcaagt cgtgatagac gcctacgtca 120

aaattcgtat gaccaaagac gaagaattca taagacagtt cgctacgtta gacgaagcgc 180

agaaggaaga gatgaagaga gagaagcgac ggatacagga gcaactgagg cggatcaaga 240

gaaaccaaga gaaggaaaag atgggaggtg ggcatacgca tatgcattcg acgcctatcg 300

taccgcctgc agggactgcg attgaaaagt tgccaaatcc aagtgactct ctttcgacac 360

ctgttacacc aacttctagt aagagtatgt cctcgtctcc tcctaaatca aggaggaaaa 420

ccaaactcaa gccagatctc aaactcaagt gtggagcctg tggaaatgtc ggccatatga 480

gaacaaacaa agcctgccct ctttatcaag ccacaactgg tcagaacccg cctattaacg 540

ttgccatgac tgaagaacaa gaagaagata tcgagaagca aattaataca gacgatgaag 600

atctcgtaaa cgtagatgga accaaagtta aattgtctgg aaagttgttg aagaattact 660

ttcagcatgc cgaggagatt aagcgtagga gcttaatgtt aaaagtccct aaggacgctt 720

taggtaaccg aaaaagaaga agaggggtgc aagatttaca ttgtgattat ttgaagaaac 780

acaacaggcc agcgaataga agacgaacgg atccggttgt cgttttgtct actattttag 840

agaatatact caatgaaatg cgagatatgc cagacgtcca gccttttctt attccctgtc 900

aatcaaaaac 910

<210>172

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>172

cagaactttt aggaacccag aaggacg 27

<210>173

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>173

gatccgttcg tcttctattc gctgg 25

<210>174

<211>776

<212>DNA

<213>Diabrotica virgifera

<400>174

cagaactttt aggaacccag aaggacgcga attcacccgc gtagaatacg tccggaagca 60

agtcgtggta gacgcctacg tcaaaattcg tatgaccaaa gacgaagaat tcataagaca 120

gttcgctacg ttagacgaag cgcagaagga agagatgaag agagagaagc gacggataca 180

ggagcaactg aggcggatca agagaaacca agagaaggaa aagatgggag gtgggcatac 240

gcatatgcat tcgacgccta tcgtaccgcc tgcagggact gcgattgaaa agttgccaaa 300

tccaagtgac tctctttcga cacctgttac accaacttct agtaagagta tgtcctcgtc 360

tcctcctaaa tcaaggagga aaaccaaact caagccagac ctcaaactca agtgtggagc 420

ctgtggaaat gtcggccata tgagaacaaa caaagcctgc cctctttatc aagccacaac 480

tggtcagaac ccgcctatta acgttgccat gactgaagaa caagaagaag atatcgagaa 540

gcaaattaat acagacgatg aagatctcgt aaacgtagat ggaaccaaag ttaaattgtc 600

tggaaagttg ttgaagaatt actttcagca tgccgaggag attaagcgca ggagtttaat 660

gttaaaagtc cctaaggacg ctttaggtaa ccgaaaaaga agaagagggg tgcaagattt 720

acattgtgat tatttgaaga agcacaatag gccagcgaat agaagacgaa cggatc 776

<210>175

<211>365

<212>DNA

<213>Diabrotica virgifera

<400>175

ggacgcgtgg ggtcaagtga agttatatta aatatattta taataattta tcctatcgtc 60

atcatgagaa gtttaatagt tgccacgatt gtacttttcg ccgtttatca ggcagtagat 120

ggtctcattg cctgtcacga aaccttttgc gacaatcaaa aatgtacaga aaacctcgtt 180

tgcaaagatt ctgaagttct ggttcccaag ggaaccattt gcggatgttg cgacgactgc 240

tacagaaaac taaatgaagg cgatgcttgc aggtcacgtt ttattggagg aggtccattc 300

agaggaaaat gtgtagatgg attagtatgt aacgctgaag gaaaatgcgc caaagcctaa 360

attat 365

<210>176

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>176

cgtttatcag gcagtagatg gtctcattg 29

<210>177

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>177

gcattttcct tcagcgttac atactaatcc 30

<210>178

<211>247

<212>DNA

<213>Diabrotica virgifera

<400>178

cgtttatcag gcagtagatg gtctcattgc ctgtcacgaa accttttgcg acaatcaaaa 60

atgtacagaa aacctcgttt gcaaagattc tgaagttctg gttcccaagg gaaccatttg 120

cggatgttgc gacgactgct acagaaaact aaatgaaggc gatgcttgca ggtcacgttt 180

tattggagga ggtccattca gaggaaaatg tgtagatgga ttagtatgta acgctgaagg 240

aaaatgc 247

<210>179

<211>870

<212>DNA

<213>Diabrotica virgifera

<400>179

acgcgtgggt tcgtattttt ttcttaacaa aagggcaaaa tgagcttttt tggaaaattg 60

ttcgggggga aaaaggaaga gatagcccct agtcctgggg aggctattca aaaactcaga 120

gagactgaag aaatgttaat aaaaaaacag gattttttag aaaagaagat agaagaattt 180

accatggtag caaagaaaaa tgcgtcgaaa aataaaagag ttgcactcca agccctcaaa 240

aagaagaaac gattggaaaa gacccaacta caaatagatg gaacccttac aactattgaa 300

atgcagaggg aagccctcga aggagctagc acaaatactg ctgtattaga ttctatgaaa 360

aatgctgcag atgcccttaa gaaagctcat aagaatttga atgtagatga tgttcacgat 420

atcatggatg acatagccga acaacacgac atagccaacg aaatcacaaa cgctattagc 480

aatcctgtcg gattcaccga cgatctggat gacgatgaat tagaaaaaga attagaagag 540

ctcgaacaag aaggattgga agaagacctg ctccaagtgc caggtccaac tcaactgccg 600

gctgtgcctg ctgatgcagt tgctactaaa ccaatcaaac cagcagctaa aaaagttgaa 660

gatgatgacg atatgaaaga attggaagcc tgggcctcgt aaaattcctg aaatctggat 720

atttgtaaac gaaatcaccc atctaagatg aaaacattat aaatatatag gtaataacag 780

ctaaaaacgt ttcaatgtag aacaagcttt tgctgaaaag gcgtcttgca aaaaatgttg 840

ataatttaga attctctata tattatatat 870

<210>180

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>180

gtcctgggga ggctattcaa aaactc 26

<210>181

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>181

caggcttcca attctttcat atcgtcatc 29

<210>182

<211>601

<212>DNA

<213>Diabrotica virgifera

<400>182

gtcctgggga ggctattcaa aaactcagag agactgaaga aatgttaata aaaaaacagg 60

attttttaga aaagaagata gaagaattta ccatggtagc aaagaaaaat gcgtcgaaaa 120

ataaaagagt tgcactccaa gccctcaaaa agaagaaacg attggaaaag acccaactac 180

aaatagatgg aacccttaca actattgaaa tgcagaggga agccctcgaa ggagctagca 240

caaatactgc tgtattagat tctatgaaaa atgctgcaga tgcccttaag aaagctcata 300

agaatttgaa tgtagatgat gttcacgata tcatggatga catagccgaa caacacgaca 360

tagccaacga aatcacaaac gctattagca atcctgtcgg attcaccgac gatctggatg 420

acgatgaatt agaaaaagaa ttagaagagc tcgaacaaga aggattggaa gaagacctgc 480

tccaagtgcc aggtccaact caactgccgg ctgtgtctgc tgatgcagtt gctactaaac 540

caatcaaacc agcagctaaa aaagttgaag atgatgacga tatgaaagaa ttggaagcct 600

g 601

<210>183

<211>860

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(804)

＜223〉n=a, c, g or t/u

<400>183

acgacaaagt aacatataat taattataaa cactgtttta ttgaaattac aaaactaaac 60

ttatcctaaa aatgatttgt aagcttatgt atatccgtaa ggtcttcaat aagttcttcc 120

attttagtac caattttttg aaacctctga cttttctgtt agcgaaaggt ttaggaaatt 180

ccacactaac tccccatcga ttatagatct agcagggttg ttttgtagtt ttctccaact 240

tttaaatgtc ctgaaagcct tgggatttag gccggcaata tgagctcctt gggaaactaa 300

tacattttgc aacataagta aacggcgata tgttttctct ggaactggca taatgtatcc 360

tatacctcca tccaaagtag caaacattgt aataaacctt ctatctgctc cagaaaaatg 420

ctttttatca tcgcctagtt cacctagttt acatttaatt ttaaagaatg tgttgacagt 480

ttggcctaaa tggaaatccg ctttccttaa tagtctttga cctcctaaag attctcttga 540

ttctggttga tacatataca tggctagatt ttgttctcca tctgatacta aaaatcctag 600

tgatgaatta tctatcatgt attctattga atatacttcg tttggtttga aatccctaga 660

taccattgac agtgttcggt actcttcttg aaaccttaac aacgcgatag atttgtagac 720

atcaccaacc aacagcaaac ttttgatagt aagtatttga tgagtataga cttgtgtatc 780

tataaatgct actccaataa atcngtatct ttgagttgcc aaatatatat cttttgtccc 840

actgctgaca caggaaacct 860

<210>184

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>184

ctctggaact ggcataatgt atcctatacc tc 32

<210>185

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>185

ctgttggttg gtgatgtcta caaatctatc g 31

<210>186

<211>399

<212>DNA

<213>Diabrotica virgifera

<400>186

ctctggaact ggcataatgt atcctatacc tccatccaaa gtagcaaaca ttgtaataaa 60

ccttctatct gctccagaaa aatgcttttt atcatcgcct agttcaccta gtttacattt 120

aattttaaag aatgtgttga cagtttggcc taaatggaaa tccgctttcc ttaatagtct 180

ttgacctcct aaagattctc ttgattctgg ttgatacata tacatggcta gattttgttc 240

tccatctgat actaaaaatc ctagtgatga attatctatc atgtattcta ttgaatatac 300

ttcgtttggt ttgaaatccc tagataccat tgacagtgtt cggtactctt cttgaaacct 360

taacaacgcg atagatttgt agacatcacc aaccaacag 399

<210>187

<211>816

<212>DNA

<213>Diabrotica virgifera

<400>187

ggtcagctgt gtacgggagt tgaagtcgag aaatgcgcct taggcatgta agtgtggctg 60

tcggagacac tcaaagacag caacatgtac gtatacggtt tcgacattac atagacgtgt 120

aatatgcgtg cgagtgcttg atcacggtga agttctggcg tgaacggtgg ctcggaggaa 180

tcatggcgtc ggtggcgggc tcgcaagaac agccgcccgc cgcgcacggc catagcttct 240

gcaagaagac cttccacaag cccacctact gccaccactg cagcgacatg ttatggggcc 300

tcatccagca gggcttcatc tgcgaagttt gcaacttcgt agtgcatgat cgctgcttaa 360

ggactgtggt atccccgtgt tccagcgtct ccgccagtca aataagaaat ccggttgcac 420

actgttggtc ggagtcgttt caccataaaa gaaaattttg caacgtatgt agaaaacggt 480

tagatgacag cgaatccatt cattgcgaaa tttgtgagta tttcgtacat gtggagtgcc 540

aggattttgc agtagctgac tgtaaagaaa atgcaactta tcttccggga aaacaactta 600

ccttcgtgcg tcatcaacat cactggcgcg aaggaaatct tccaagcaat tcccaagtgt 660

gctctctgta aaaagacgtg ctggactact gaatgtttat cccggataca gatgcgaatg 720

gtggtgaatg acgagccatg cctcctgtcc tacgagtatt aatagtgaat ggactttttg 780

gaattctgga acccattcat ctacccccgc aggctg 816

<210>188

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>188

ccttaggcat gtaagtgtgg ctgtcg 26

<210>189

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>189

ctcgtaggac aggaggcatg gct 23

<210>190

<211>717

<212>DNA

<213>Diabrotica virgifera

<400>190

ccttaggcat gtaagtgtgg ctgtcggaga tactcaaaga cagcaacatg tacgtatacg 60

gtttcgacat tacatagacg tgtaatatgc gtgcgagtgc ttgatcacgg tgaagttctg 120

gcgtgaacgg tggctcggag gaatcatggc gtcggtggcg ggctcgcaag aacagccgcc 180

cgccgcgcac ggccatagct tctgcaagaa gaccttccac aagcccacct actgccacca 240

ctgcagcgac atgttatggg gcctcatcca gcagggcttc atctgcgaag tttgcaactt 300

cgtagtgcat gatcgctgct taaggactgt ggtatccccg tgttccagcg tctccgccag 360

tcaaataaga aatccggttg cacactgttg gtcggagtcg tttcaccata aaagaaaatt 420

ttgcaacgta tgtagaaaac ggttagatga cagcgaatcc attcattgcg aaatttgtga 480

gtatttcgta catgtggagt gccaggattt tgcagtagct gactgtaaag aaaatgcaac 540

ttatcttccg ggaaaacaac ttaccttcgt gcgtcatcaa catcactggc gcgaaggaaa 600

tcttccaagc aattccaagt gtgctctctg taaaaagacg tgctggacta ctgaatgttt 660

atccggatac agatgcgaat ggtgtggaat gacgagccat gcctcctgtc ctacgag 717

<210>191

<211>826

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(752)

＜223〉n=a, c, g or t/u

<400>191

gcagtggtta gtgcagtttt gtgatttaag aagattacga tggttactac cacgaatgcg 60

gtcactagta ctagcatgat tcaaggaaat agtacgacgc ctacatctaa cacccctggc 120

acaggcgaga ataataatgg tgttaagaga attagtgata atagaaggag taacaagcct 180

attatggaga aaaggcggag ggcgaggata aataactgtc tcaatgagtt gaaaacgctc 240

atattggacg ccatgaaaaa agatcctgcc agacattcaa agctggaaaa agctgacatc 300

ctggagatga cagtcaagca tttacagaac cttcaaagac agcaagtcgc tatgtcagcg 360

gcagcagatc ccttagtagt taataaattc agagcagggt ttagcgagtg cgcttcagaa 420

gtcggcagat ttccaggtct agatccaatc gtccgacgaa ggttactcca gcatctagcc 480

aactgtctca gccaaggttc caaaccacaa gaagttcctc aagtacaagt tcacatactt 540

ccaaacactc aaaaaccttt agaacagcaa gtaccccaaa gcagtggcat tattcttagt 600

aacggtaacg gtactggagt tcaactagta ccaacaagac taccgaatgg agatttagct 660

ctggtacttc ctacgacctt acaaacacag ccccagtatt caccggcggc agtaccatta 720

ttagtgccga tagtaccgga aagaaccgct tncaccacat cagcgaccag cagttactcg 780

aattatttct ccagtcatag tccagagcct atggagtcta tgtaca 826

<210>192

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>192

gcagttttgt gatttaagaa gattacgatg g 31

<210>193

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>193

gctgtgtttg taaggtcgta ggaagtacca 30

<210>194

<211>679

<212>DNA

<213>Diabrotica virgifera

<400>194

gcagttttgt gatttaagaa gattacgatg gttactacca cgaatgcggt cactagtact 60

agcatgattc aaggaaatag tacgacgcct acatctaaca cccctggcac aggcgagaat 120

aataatggtg ttaagagaat tagtgataat agaaggagta acaagcctat tatggagaaa 180

aggcggaggg cgaggataaa taactgtctc aatgagttga aaacgctcat atcggacgcc 240

atgaaaaaag atcctgccag acattcaaag ctggaaaaag ctgacatcct ggagatgaca 300

gtcaagcatt tacagaacct tcaaagacag caagtcgcta tgtcagcggc agcagatccc 360

ttagtagtta ataacttcag agcagggttt agcgagtgcg cttcagaagt cggcagattt 420

ccaggtctag atccaatcgt ccgacgaagg ttactccagc atctagccaa ctgtctcagc 480

caaggttcca aaccacaaga agttcctcaa gtacaagttc acatacttcc aaacactcaa 540

aaacctttag aacagcaagt accccaaagc agtggcatta ttcttagtaa cggtaacggt 600

actggagttc aactagtacc gacaagacta ccgaatggag atttagctct ggtacttcct 660

acgaccttac aaacacagc 679

<210>195

<211>873

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(709)..(772)

＜223〉n=a, c, g or t/u

<400>195

cgacgaagtc accgtgggta aattctacgc gactttcctc attcaggatt acttcaggcg 60

gttcaagaag agaaaagagc aggagatgag ggacggagat aaagatattc agaataccgt 120

tacacttcaa gctgggttgc gtacgttaca tgaggctggt ccagaactga aacgcgccat 180

ctcaggcaat ctagatgaat taattgatga caatcccgaa ccaatgcatc gtagaaatca 240

ttcactcttc ggaagtgtat ggtcttcaat gagacgaggt catactttca atagagcgaa 300

atctctgaag ttaaattcaa cccaaatcaa aattacaccg gcttccagcg tcgactatct 360

tccatacaat tctattaaga atgccgtcac agaagggatg aatcacatta cttccatgac 420

gaaaaacgtt caaaaccgtg ttagcgctag ttccttgaac aatcaggata actataagga 480

tgaagaaatt cctctgaggt ctcttagtaa tctgcataat ggagatgaga agaataacta 540

cacggtcatc gataaatctg gcttcctgca ccctaactac aaggttggag caggtgaggc 600

gaatggtcac gtgggaccag agcgtcttag ccattcactg cctggcagtc cggccgaccg 660

tagacttgct tccttcgaag tcgtaggctc tgcggaaagc ctggtaggnc gaatcctagc 720

cgagcaaggc ttaggtaagt actgtgatca ggatttcgtg cgctacacct nncgaagaga 780

tgcagaggca ttggatatga ctcaggagga aatggatcgc gcagcccacc agattctttt 840

cacagagaaa cttatacatc atcctctgca gca 873

<210>196

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>196

cgacgaagtc accgtgggta aattc 25

<210>197

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>197

cgaaggaagc aagtctacgg tcgg 24

<210>198

<211>677

<212>DNA

<213>Diabrotica virgifera

<400>198

cgacgaagtc accgtgggta aattctacgc gactttcctc attcaggatt acttcaggcg 60

gttcaagaag agaaaagagc aggagatgag ggacggagat aaagatattc agaataccgt 120

tacacttcaa gctgggttgc gtacgttaca tgatgctggt ccagaactga aacgcgccat 180

ctcaggcaat ctagatgaat taattgatga caatcccgaa ccaatgcatc gtagaaatca 240

ttcactcttc ggaagtgtat ggtcttcaat gagacgaggt catactttca atagagcgaa 300

atctctgaag ttaaattcaa cccaaatcaa aattacaccg gcttccagcg tcgactatct 360

tccatacaat tctattaaga atgccgtcac agaagggatg aatcacatta cttccatgac 420

gaaaaacgtt caaaaccgtg ttagcgctag ttccttgaac aatcaggata actataagga 480

tgaagaaatt cctctgaggt ctcttagtaa tctgcataat ggagatgaga agaataacta 540

cacggtcatc gataaatctg gcttcctgca ccctaactac aaggttggag caggtgaggc 600

gaatggtcac gtgggaccag agcgtcttag ccattcactg cctggcagtc cggccgaccg 660

tagacttgct tccttcg 677

<210>199

<211>418

<212>DNA

<213>Diabrotica virgifera

<400>199

acggaaacac agttacctgt atcgttccga catttgattt tgcccgagaa aaacttccct 60

cctactgaat tgctggactt gcaaccgctg cctattactg ctttgagaaa cgaacaattt 120

gagtctttat acgccgataa gtttccacag tttaatccta tacaaacaca ggttttcaac 180

tcagtataca atggagacga caacatattc ataggcgctc ccacgggatc aggaaagacc 240

acaatagcaa aatttgccat cctcagactc ttcgattgaa atcctgaagg tcgatgtgtt 300

tatctagttc ctaaggacgc cctcgctgaa ctgattttcg ccgattggca taataagttt 360

ggacaggcct tggcaaagaa agtggtattg ctgactggag aaactggtac tgatctaa 418

<210>200

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>200

cacagttacc tgtatcgttc cgacatttg 29

<210>201

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>201

gatcagtacc agtttctcca gtcagcaata c 31

<210>202

<211>408

<212>DNA

<213>Diabrotica virgifera

<400>202

cacagttacc tgtatcgttc cgacatttga ttttgcccga gaaaaacttc cctccgactg 60

aattgctgga cttgcaaccg ctgcctatta ctgctttgag aaacgaacaa tttgagtctt 120

tatacgccga taagtttcca cagtttaatc ctatacaaac acaggttttc aactcagtat 180

acaatggaga cgacaacata ttcataggcg ctcccacggg atcaggaaag accacaatag 240

cagaatttgc catcctcaga ctcttcgatc gaaatcctga aggtcgatgt gtttatctag 300

ttcctaagga cgccctcgct gaactgattt tcgccgattg gcataataag tttggacagg 360

ccttggcaaa gaaagtggta ttgctgactg gagaaactgg tactgatc 408

<210>203

<211>735

<212>DNA

<213>Diabrotica virgifera

<400>203

cactgtcatt cgtcattctc atacattttt tttgggtact ttgggttgta cttgggataa 60

aagacctttt tgtatttcca aatacattgt catataaatt aaaaaagaaa tggctgccat 120

cagatctatg tctcgccttc ttattaatag atcacttcaa accactgcaa agaggaccta 180

tgccgaccaa atgaacttca catttgcagc tggaaatcag gtttactata ctgcaacttc 240

agttaaacaa gtagatgtac catctttctc tggtagcttt ggtatccttc ccgcccatgt 300

acccacactg gctgtgctaa aacctggagt agtcactgtt tacgaagatg gtggtaatgt 360

taagaaaatc tttgtttcca gtggtactgt caccatcaat gatgattcat cagttcagat 420

tcttgctgaa gaagcccacc cagtagaaaa cattgatgtc aatgcagcaa gagacatcct 480

taccaaagcc cagagccagt tatcatctgc aagtacagat gaggcgaaag ctgaagcctc 540

cattgctgtt gaagtgggag aagcgttagt caaagcggct gaataataat ttaatttttg 600

taattttatg taaatttaat aaattagtgt ctcaatggta ctgttcattt taaaacaaca 660

acatattaga tctatacctc tatgagttga caatttttac aaaaacgaga atcaatccaa 720

atacataatt tttgt 735

<210>204

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>204

ggtactttgg gttgtacttg ggataaaaga c 31

<210>205

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>205

gctttgacta acgcttctcc cacttca 27

<210>206

<211>542

<212>DNA

<213>Diabrotica virgifera

<400>206

ggtactttgg gttgtacttg ggataaaaga cctttttgta tttccaaata cattgtcata 60

taaattaaaa aagaaatggc tgccatcaga tctatgtctc gccttcttat taatagatca 120

cttcaaacca ctgcaaagag gacctatgcc gaccaaatga acttcacatt tgcagctgga 180

aatcaggttt actatactgc aacttcagtt aaacaagtag atgtgccatc tttctctggt 240

agctttggta tcctccccgc ccatgtaccc acactggctg tgctaaaacc tggagtagtc 300

actgtttacg aagatggtgg taatgttaag aaaatctttg tttccagtgg tactgtcacc 360

atcaatgatg attcatcagt tcagattctt gctgaagaag cccacccagt agaaaacatt 420

gatgtcaatg cagcaagaga catccttacc aaagcccaga gccagttatc atctgcaagt 480

acagatgagg cgaaagctga agcctccatt gccgttgaag tgggagaagc gttagtcaaa 540

gc 542

<210>207

<211>686

<212>DNA

<213>Diabrotica virgifera

<400>207

tatatttaat aattaataca gatttttaag tatggcacca aaggaaaaag atgatgaaat 60

cgtatcaata aataaatggg atggagtagc cgtcaaaaat gcattagatg atgcagttaa 120

agaggtactt acaaagaaat acaattatgc tgaaaatttt gctctcatgg atggtaggtt 180

agttatatgt ggcatagctg taggtgttgc aatgtttgcc ttgatttggg attatttgta 240

cccattccca caatctaagc ctattttaat attttctgtt actacatatt tttttatgat 300

gggaatttta actttatata ctacatacaa agagaaagga atctttgctg tttgtattca 360

gaaagatagt tctaagaagg aaaatacttg ggaagctagc tcttatattc aaaagtatga 420

tgataagtat actttgaatc ttgcatatat tgatggaaaa acacgccaaa gaagagagca 480

gagtattaaa aggagtattg ccaggtacat agaccagcaa ggaactgtag ttaaagaaaa 540

tgttgaagat gaaatcacca aaatgcacaa ttctctacta agtgagagga aagaaaaatg 600

atgtacacag agactaccta ctaaaagaag ttgacgtgta aattatttca ttttttatat 660

ttttcaataa gtttttttgg tttgag 686

<210>208

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>208

gtatggcacc aaaggaaaaa gatgatga 28

<210>209

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>209

cattttggtg atttcatctt caacattttc 30

<210>210

<211>536

<212>DNA

<213>Diabrotica virgifera

<400>210

gtatggcacc aaaggaaaaa gatgatgaaa tcgtatcaat aaataaatgg gatggagtgg 60

ccgtcaaaaa tgcattagat gatgcagtta aagaggtact tacaaagaaa tacaattatg 120

ctgaaaattt tgctctcatg gatggtaggt tagttatatg tggcatagct gtaggtgttg 180

caatgtttgc cttgatttgg gattatttgt acccattccc acaatctaag cctattttaa 240

tattttctgt tactacatat ttttttatga tgggaatttt aactttatat actacataca 300

aagagaaagg aatctttgct gtttgtattc agaaagatag ttctaagaag gaaaatactt 360

gggaagctag ctcttatatt caaaagtatg atgataagta tactttgaat cttgcatata 420

ttgatggaaa aacacgccaa agaagagagc agagtattaa aaggagtatt gccaggtaca 480

tagaccagca aggaactgta gttaaagaaa atgttgaaga tgaaatcacc aaaatg 536

<210>211

<211>901

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(707)

＜223〉n=a, c, g or t/u

<400>211

cggacgcgtg ggctaatagc attgtgttat ccatttttag cttctagtgt caatgagtac 60

attccattga ttattaaaaa tctagatgaa catactgatg gggtctgtaa taatgctgct 120

tgggtgattg gaaagttgtg tgctgtgatg ggcaatgcaa tccaaccttt ccttccagaa 180

atccttaatg cctttgttca tatattaaga acaccagctg tagctcgtac catgcacaaa 240

actgtagcta tttccttatg catcgtattc tatgtgtgcc ccgatgtgac tattccagaa 300

atggatgtga ttgttaagaa ttgctgtatg ttagtgagaa atgttaggga ttcagatgag 360

aaggatcttg cttttcgagg cttgtgtgag gtgattataa ggcatccaga attcaacaag 420

aactacttca tcttcttttg tgatgctgca gcttcctgga tcaatatccg tgtagatttg 480

aagcagacaa ttcgcaatgt tctattggca tttaaacaac aatgtggtga ggataactgg 540

cgccaatttt attgtcagtt tcctgaagct cttaaactta aacttcaaga gttatatggg 600

gtatgaaata aacaatctac aaaaaacggg gaattcgaca tatgggtggg aaagcttgag 660

tgcacctgcc ttgagtttat cgcgtaatag tatttaaata tcgtccngtg agggaggggc 720

gtcttgccgg tatcttagag tcacgcgtta ataaaaaaat cgagaaggat ttttttagac 780

agattaattt ttttgtttta tgggtagttt cttagtaaat caactaaata atgtttattt 840

aattattttt taaggaattt tataacaaat atggtaattt atattgtgga agtgttttta 900

t 901

<210>212

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>212

cgtgggctaa tagcattgtg ttatcca 27

<210>213

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>213

gaacattgcg aattgtctgc ttcaaatc 28

<210>214

<211>496

<212>DNA

<213>Diabrotica virgifera

<400>214

cgtgggctaa tagcattgtg ttatccattt ttagcttcta gtgtcaatga gtacattcca 60

ttgattatta aaaatctaga tgaacatact gatggggtct gtaataatgc tgcttgggtg 120

attggaaagt tgtgtgctgt gatgggcaat gcaatccaac ctttccttcc agaaatcctt 180

aatgcctttg ttcatatatt aagaacacca gctgtagctc gtaccatgca caaaactgta 240

gctatttcct tatgcatcgt attctatgtg tgccccgatg tgactattcc agaaatggat 300

gtgattgtta agaattgctg tatgttagtg agaaatgtta gggattcaga tgagaaggat 360

cttgcttttc gaggcttgtg tgaggtgatt ataaggcatc cagaattcaa caagaactac 420

ttcatcttct tttgtgatgc tgcagcttcc tggatcaata tccgtgtaga tttgaagcag 480

acaattcgca atgttc 496

<210>215

<211>916

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(870)

＜223〉n=a, c, g or t/u

<400>215

ctgtcgggat gtaagagcta tgattgaaag gcaggcgtct agacattgcg atcggcaggc 60

agaatagcag gaaagcaaga caaatcctaa agatcaagcg tcatcgggtt tagaccggca 120

tacagcatgc gaccgcatgg gcataaggag atcgcagtga tcgcacccgt atcggctaat 180

agacacagac acagacacgg tgtgttcggt atttttagtt agtctgttcg cgaccggcga 240

agaagtagcg cgtgtgttta aatagagagt tatggtgata acgtcaacgt gtgttgtgac 300

ggtgtttaag atcaacggtc ggcaccggag tcgacaacgc cagtgagcaa gcagtgcgac 360

actatgtccc agtcatggtg aaaacgtttc tgggactgtt agctgcttca atttgtggtc 420

tcgtatacgc tgctccatta gctgactctt gcaataatct gacccctttt cgaacagata 480

gcacaacccc tacttcttca gaagagtcat tgctagagga tccagcagca cgcaacgaaa 540

gaagcacaaa tctctctcac gtcactggta ccgccagaaa aatacaaatg ttcatcaaga 600

atcgacattt acaaattctt cctgacggga cagtcaacgg aaccactgac gacactagtg 660

cttattcgat attacaaagg acgacggtgg gaattggtca aatgaaaatt caaggtgtgg 720

ttacctgtca gtatttgtgc atggccaggt gtggattgct ctatggatcg agagaatttg 780

gacaagaatg cgtattcaat gagaccatcg agcaatccaa ctacaacacg tattcctccc 840

agtaatactc aaacgagaaa agaacccttn atctagcact gaatcaaaga ggccaatcga 900

gaaaggtcgt cctacg 916

<210>216

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>216

gctatgattg aaaggcaggc gtctag 26

<210>217

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>217

ggtctcattg aatacgcatt cttgtcc 27

<210>218

<211>789

<212>DNA

<213>Diabrotica virgifera

<400>218

gctatgattg aaaggcaggc gtctagacat tgcgatcggc aggcagaata gcaggaaagc 60

aagacaaatc ctaaagatca agcgtcatcg ggtttagacc ggcatacagc atgcgaccgc 120

atgggcataa ggagatcgca gtgatcgcac ccgtatcggc taatagacac agacacagac 180

acggtgtgtt cggtattttt agttagtctg ttcgcgaccg gcgaagaagt agcgcgtgtg 240

tttaaataga gagttatggt aataacgtca acgtgtgttg tgacggtgtt taagatcaac 300

ggtcggcacc ggagtcgaca acgccagtga gcaagcagtg cgacactatg tcccagtcat 360

ggtgaaaacg tttctgggac tgttagctgc ttcaatttgt ggtctcgtat acgctgctcc 420

atagctgact cttgcaataa tctgacccct tttcgaacag atagcacaac ccctacttct 480

tcagaagagt cattgctaga ggatccagca gcacgcaacg aaagaagcac aaatctctct 540

cacgtcactg gtaccgccag aaaaatacaa atgttcatca agaatcgaca tttacaaatt 600

cttcctgacg ggacagtcaa cggaaccact gacgacacta gtgcttattc gatattacaa 660

aggacgacgg tgggaattgg tcaaatgaaa attcaaggtg tggttacctg tcagtatttg 720

tgcatggcca ggtgtggatt gctctatgga tcgagagaat ttggacaaga atgcgtattc 780

aatgagacc 789

<210>219

<211>2176

<212>DNA

<213>Diabrotica virgifera

<400>219

caaaattatt taagtagtgg agtccaaata gactatttct tcttttgggc tttttctgca 60

gcttttgtga cttttccagt agtatcttca tcttcagtga atacaccaat acgtctgcaa 120

atggcttcag cagtagcttt gttgtcacca gtgataacaa taactctgat accagcagcg 180

cggcattgaa caatggagtc aaatacttct ttacgtgggg gatccaacat accaacaaca 240

ccaacgaatg taagattgac ttcatatgtg tagaatttgg ttgaatcgcc aaggtccatt 300

tcgtctggtt tcatggggtt atcagcagtt gctagggcaa gacaacgcaa tgtgtcgcgt 360

ccacaaccat attgagcggt aagatcagtg attctctttt taagagtgct ggtaagtggt 420

actttttgag atccaacacg agcgtgtgta cacctttcca aaacaccttc tggagctcct 480

ttaacgaaga gtttaggtcc attaccaata cgagatggtt tgaggggtgt gcagtaggaa 540

gacatagatt tacgatcacg tgagaattcc aaagtgaatt ccttcttcca tttagtttca 600

atgtcttgtc taacacagat ggcaacctgt ctacgatctc cagttttact gacagagaag 660

ggattcattt tttctgctaa tacaatgaga gcagtttcag tagcttcacc aactttttcg 720

aaagcttgtt tgaattcatt gaaatcaatg gcggagtcat tgcacataat acaaatggta 780

ccaatttcat gtaatgcgtc aaattctgca catttaactt tttgtccttt aaggaatact 840

tcaccaatag gttcataagt ggagccagtg atttcaaatt catggaaaga gctgtcgctg 900

ccttcaactt tatcgaaaat aaacatacgt gagactgaca tttggttggt ggttaaggta 960

ccagttttat cggagcagat gacagatgtg caaccaagag tttctacaga tggaagggac 1020

cttacaatag cgtttttctt agccatacga cgagtaccaa gagcaagaca tgtagtaatg 1080

acagctggta gaccttcagg aatggcagcg acagccaaag ctacagcgat tttgaagtag 1140

tagacagcac ctttaatcca tgaaccacca tgggctgggt cattgaaatg tccaatgtta 1200

atagcccata cagcaacaca gatgactgaa atgactttgg agagttgttc accgaattcg 1260

tccaattttt gttgaagggg agtcttaatt tcttccgttt ctgacatttc agttctgatt 1320

ttaccaatgg cggtgttaag accagttcca ataacaatac cacgtgcttt accagcagca 1380

acattggtac ctgagaaaag aatgtttttc ttgtcttggt tgacagctct tgggtcggga 1440

atggcatcgg tgtgtttgat cacagaaacg gattcaccag tcaggataga ctgatcgata 1500

cggagtgttg ttgagaaaat tttaactaaa cgaatatcag cgggaatttt gtcacccact 1560

gacacttcta ctatatctcc gggtacgatt tctttcgccc ggattttctg aataccggat 1620

ttgtcttgtc ggacaacttt gcccatttct ggttcatatt ctttaagagc ttcaatggcg 1680

gattcagcat ttctttcctg ccatacacct actactgcgt ttgcaataag aattaaaaga 1740

attacaaaag gttctacgaa cgcggtgaat gcgccatcgt gttcttcaaa taaagctaat 1800

acaaaggaaa ttatagcggc taataataaa atcttgacta gaagatcatc aaactgttct 1860

aaaactaatt gccatatgga ttttccttct tccgcaggaa gttcattggg tccatattta 1920

gcttgattct ttttgacttg atcaagtgaa agacctcgtt cttgatcagc gtcgaagtac 1980

ctaagtactt cgtccactgt ttttgcgtga ccatcctcca ttctgcccag ttgtcacttc 2040

gcttgttatc acaaaaatgg tcccagtagt tatcacttga cgaaaacttt ggtggattat 2100

caaaggttaa caacgacacg gtattgttta ggcccccatt taagagtagc acaaaattgt 2160

cacagaagtt tagagc 2176

<210>220

<211>33

<212>DNA

<213>Diabrotica virgifera

<400>220

gctctaaact tctgtgacaa ttttgtgcta ctc 33

<210>221

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>221

gaaatgtcca atgttaatag cccatacagc 30

<210>222

<211>992

<212>DNA

<213>Diabrotica virgifera

<400>222

gctctaaact tctgtgacaa ttttgtgcta ctcttaaatg ggggcctaaa caataccgtg 60

tcgttgttaa cctttgataa tccaccaaag ttttcgtcaa gtgataacta ctgggaccat 120

ttttgtgata acaagcgaag tgacaactgg gcagaatgga ggatggtcac gcaaaaacag 180

tggacgaagt acttaggtac ttcgacgctg atcaagaacg aggtctttca cttgatcaag 240

tcaaaaagaa tcaagctaaa tatggaccca atgaacttcc tgcggaagaa ggaaaatcca 300

tatggcaatt agttttagaa cagtttgatg atcttctagt caagatttta ttattagccg 360

ctataatttc ctttgtatta gctttatttg aagaacacga tggcgcattc accgcgttcg 420

tagaaccttt tgtaattctt ttaattctta ttgcaaacgc agtagtaggt gtatggcagg 480

aaagaaatgc tgaatccgcc attgaagctc ttaaagaata tgaaccagaa atgggcaaag 540

ttgtccgaca agacaaatcc ggtattcaga aaatccgggc gaaagaaatc gtacccggag 600

atatagtaga agtgtcagtg ggtgacaaaa ttcccgctga tattcgttta gttaaaattt 660

tctcaacaac actccgtatc gatcagtcta tcctgactgg tgaatccgtt tctgtgatca 720

aacacaccga tgccattccc gacccaagag ctgtcaacca agacaagaaa aacattcttt 780

tctcaggtac caatgttgct gctggtaaag cacgtggtat tgttattgga actggtctta 840

acaccgccat tggtaaaatc agaactgaaa tgtcaggaac ggaagaaatt aagactcccc 900

ttcaacaaaa attggacgaa ttcggtgaac aactctccaa agtcatttca gtcatctgtg 960

ttgctgtatg ggctattaac attggacatt tc 992

<210>223

<211>2176

<212>DNA

<213>Diabrotica virgifera

<400>223

caaaattatt taagtagtgg agtccaaata gactatttct tcttttgggc tttttctgca 60

gcttttgtga cttttccagt agtatcttca tcttcagtga atacaccaat acgtctgcaa 120

atggcttcag cagtagcttt gttgtcacca gtgataacaa taactctgat accagcagcg 180

cggcattgaa caatggagtc aaatacttct ttacgtgggg gatccaacat accaacaaca 240

ccaacgaatg taagattgac ttcatatgtg tagaatttgg ttgaatcgcc aaggtccatt 300

tcgtctggtt tcatggggtt atcagcagtt gctagggcaa gacaacgcaa tgtgtcgcgt 360

ccacaaccat attgagcggt aagatcagtg attctctttt taagagtgct ggtaagtggt 420

actttttgag atccaacacg agcgtgtgta cacctttcca aaacaccttc tggagctcct 480

ttaacgaaga gtttaggtcc attaccaata cgagatggtt tgaggggtgt gcagtaggaa 540

gacatagatt tacgatcacg tgagaattcc aaagtgaatt ccttcttcca tttagtttca 600

atgtcttgtc taacacagat ggcaacctgt ctacgatctc cagttttact gacagagaag 660

ggattcattt tttctgctaa tacaatgaga gcagtttcag tagcttcacc aactttttcg 720

aaagcttgtt tgaattcatt gaaatcaatg gcggagtcat tgcacataat acaaatggta 780

ccaatttcat gtaatgcgtc aaattctgca catttaactt tttgtccttt aaggaatact 840

tcaccaatag gttcataagt ggagccagtg atttcaaatt catggaaaga gctgtcgctg 900

ccttcaactt tatcgaaaat aaacatacgt gagactgaca tttggttggt ggttaaggta 960

ccagttttat cggagcagat gacagatgtg caaccaagag tttctacaga tggaagggac 1020

cttacaatag cgtttttctt agccatacga cgagtaccaa gagcaagaca tgtagtaatg 1080

acagctggta gaccttcagg aatggcagcg acagccaaag ctacagcgat tttgaagtag 1140

tagacagcac ctttaatcca tgaaccacca tgggctgggt cattgaaatg tccaatgtta 1200

atagcccata cagcaacaca gatgactgaa atgactttgg agagttgttc accgaattcg 1260

tccaattttt gttgaagggg agtcttaatt tcttccgttt ctgacatttc agttctgatt 1320

ttaccaatgg cggtgttaag accagttcca ataacaatac cacgtgcttt accagcagca 1380

acattggtac ctgagaaaag aatgtttttc ttgtcttggt tgacagctct tgggtcggga 1440

atggcatcgg tgtgtttgat cacagaaacg gattcaccag tcaggataga ctgatcgata 1500

cggagtgttg ttgagaaaat tttaactaaa cgaatatcag cgggaatttt gtcacccact 1560

gacacttcta ctatatctcc gggtacgatt tctttcgccc ggattttctg aataccggat 1620

ttgtcttgtc ggacaacttt gcccatttct ggttcatatt ctttaagagc ttcaatggcg 1680

gattcagcat ttctttcctg ccatacacct actactgcgt ttgcaataag aattaaaaga 1740

attacaaaag gttctacgaa cgcggtgaat gcgccatcgt gttcttcaaa taaagctaat 1800

acaaaggaaa ttatagcggc taataataaa atcttgacta gaagatcatc aaactgttct 1860

aaaactaatt gccatatgga ttttccttct tccgcaggaa gttcattggg tccatattta 1920

gcttgattct ttttgacttg atcaagtgaa agacctcgtt cttgatcagc gtcgaagtac 1980

ctaagtactt cgtccactgt ttttgcgtga ccatcctcca ttctgcccag ttgtcacttc 2040

gcttgttatc acaaaaatgg tcccagtagt tatcacttga cgaaaacttt ggtggattat 2100

caaaggttaa caacgacacg gtattgttta ggcccccatt taagagtagc acaaaattgt 2160

cacagaagtt tagagc 2176

<210>224

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>224

gtggttcatg gattaaaggt gctgtctac 29

<210>225

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>225

caccagtgat aacaataact ctgataccag ca 32

<210>226

<211>1023

<212>DNA

<213>Diabrotica virgifera

<400>226

gtggttcatg gattaaaggt gctgtctact acttcaaaat cgctgtagct ttggctgtcg 60

ctgccattcc tgaaggtcta ccagctgtca ttactacatg tcttgctctt ggtactcgtc 120

gtatggctaa gaaaaacgct attgtaaggt cccttccatc tgtagaaact cttggttgca 180

catctgtcat ctgctccgat aaaactggta ctttaaccac caaccaaatg tcagtctcac 240

gtatgtttat tttcgataaa gttgaaggca gcgacagctc tttccatgaa tttgaaatca 300

ctggctccac ttatgaacct attggtgaag tattccttaa aggacaaaaa gttaaatgtg 360

cagaatttga cgcattacat gaaattggta ccatttgtat tatgtgcaat gactccgcca 420

ttgatttcaa tgaattcaaa caagctttcg aaaaagttgg tgaagctact gaaactgctc 480

tcattgtatt agcagaaaaa atgaatccct tctctgtcag taaaactgga gatcgtagac 540

agattgccat ctgtgttaga caagacattg aaactaaatg gaagaaggaa ttcactttgg 600

aattctcacg tgatcgtaaa tctatgtctt cctactgcac acccctcaaa ccatctcgta 660

ttggtaatgg acctaaactc ttcgttaaag gagctccaga aggtgttttg gaaaggtgta 720

cacacgctcg tgttggatct caaaaagtac cacttaccag cactcttaaa aagagaatca 780

ccgatcttac cgctcaatat ggttgtggac gcgacacatt gcgttgtctt gccctagcaa 840

ctgctgataa ccccatgaaa ccagacgaaa tggaccttgg cgattcaacc aaattctaca 900

catatgaagt caatcttaca ttcgttggtg ttgttggtat gttggatccc ccacgtaaag 960

aagtatttga ctccattgtt caatgccgcg ctgctggtat cagagttatt gttatcactg 1020

gtg 1023

<210>227

<211>1929

<212>DNA

<213>Diabrotica virgifera

<400>227

cacttaattt tcagtactgg ggcgactggc aaaaaaatgt ctttgctttc cgcacgatta 60

gctgcctcat tggcaagaaa tgcccctaaa aatgtaacac agattgcggg agctgtcttc 120

cctgctgcat ccgtcgcagc ccggaacttc catgctactc cacaaaacaa aggtgctgaa 180

atttcatcca tccttgaaga gcgcatcctt ggtgctgcac caaaagccaa cctcgatgaa 240

acaggacgag tcttaagtat tggtgacggt atcgcccgtg tatatgggtt gaacaacatc 300

caagccgatg agatggtgga attctcctcc ggtctcaaag gtatggccct taacttggaa 360

cctgacaacg ttggtgttgt agtatttggt aacgacaaac tcattaagga aggtgacatt 420

gtcaagagaa ctggtgccat tgtagatgtg cctgtaggtg accaaatttt gggacgtgta 480

gttgatgcct taggtaaccc aattgatggc aaaggaccat tgaacaccaa acaacgtttc 540

cgtgtaggta tcaaagcccc tggtatcatt ccacgtatct ctgtaagaga acctatgcag 600

tctggaatta aggctgtaga ttctcttgta ccaattggtc gtggtcagcg tgagttgatc 660

attggtgatc gtcaaactgg taaaactgct ttggctattg atacaatcat caaccaaaaa 720

cgattcaacg atgctgaaga cgaaaagaag aaattgtact gcatttacgt cgccatcggt 780

caaaagagat ctactgtggc tcaaatcttg aagcgtctca cagacactgg tgccatcaac 840

tacacaatca ttgtatcagc tactgcatct gatgctgcac ctttgcaata cctcgcccca 900

tactccggat gtgctatggg tgaatttttc agagataatg gcaaacatgc cctcatcatc 960

tatgatgatt tgtccaaaca agccgtagcc taccgtcaga tgtctctgtt gcttcgtcgt 1020

cctccagggc gtgaagccta ccctggtgat gtattctacc ttcactcccg tcttttggaa 1080

cgtgccgcca agatgtctga agcccacggt ggtggttcat tgactgcctt gcccgtaatt 1140

gaaacccaag ctggtgatgt atcagcctac attccaacca atgtcatttc catcactgat 1200

ggacaaatct tcttggaaac tgaattgttc tacaaaggta tcagacctgc cattaacgta 1260

ggtttgtctg tatccagagt aggatcagct gctcaaacca aggccatgaa acaggttgct 1320

ggttccatga aactggaatt ggcccaatac cgtgaagtag ctgcttttgc ccaattcggt 1380

tccgatcttg atgctgctac tcagcaattg ttgaacagag gtgtacgtct tactgaattg 1440

ttgaaacaag gtcaatacgt acccatggct attgaagaac aagtagccat catctactgt 1500

ggtgtcagag gacatttgga caaagttgat ccatcaaaga ttacagtatt tgaaaaacaa 1560

ttctcagaac acattaagac tagccacaaa aacatcttgc agagcattgc caaggaagga 1620

aaaatcacag acgatactga tgcccaactc aagaaagtcg ttcaagattt cttggccagc 1680

ttctctggtt aaatataaat aatacaatta ttattaatac ctaaatcgtc acttttgttg 1740

atgtgatctg taaacgttta agaacttcat gagctgtaat cctttcaaat tagaataatt 1800

aggcaggact cctatgttat ccaataaggc acgtgaaaga attttctttt ctccatttta 1860

caatatttat cgatcattgt tttttaagga tatcttattt gtaataataa aaaataaact 1920

ttatatttt 1929

<210>228

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>228

gtctttgctt tccgcacgat tagc 24

<210>229

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>229

gttgatggca ccagtgtctg tgagac 26

<210>230

<211>799

<212>DNA

<213>Diabrotica virgifera

<400>230

gtctttgctt ccgcacgatt agctgcctca ttggcaagaa atgcccctaa aaatgtaaca 60

cagattgcgg gagctgtctt ccctgctgca tccgtcgcag cccggaactt ccatgctact 120

ccacaaaaca aaggtgctga aatttcatcc atccttgaag agcgcatcct tggtgctgca 180

ccaaaagcca acctcgatga aacaggacga gtcttaagta ttggtgacgg tatcgcccgt 240

gtatatgggt tgaacaacat ccaagccgat gagatggtgg aattctcctc cggtctcaaa 300

ggtatggccc ttaacttgga acctgacaac gttggtgttg tagtatttgg taacgacaaa 360

ctcattaagg aaggtgacat tgtcaagaga actggtgcca ttgtagatgt gcctgtaggt 420

gaccaaattt tgggacgtgt agttgatgcc ttaggtaacc caattgatgg caaaggacca 480

ttgaacacca aacaacgttt ccgtgtaggt atcaaagccc ctggtatcat tccacgtatc 540

tctgtaagag aacctatgca gtctggaatt aaggctgtag attctcttgt accaattggt 600

cgtggtcagc gtgagttgat cattggtgat cgtcaaactg gtaaaactgc tttggctatt 660

gatacaatca tcaaccaaaa acgattcaac gatgctgaag acgaaaagaa gaaattgtac 720

tgcatttacg tcgccatcgg tcaaagagat ctactgtggc tcaaatcttg gagcgtctca 780

cagacactgg tgccatcac 799

<210>231

<211>1519

<212>DNA

<213>Diabrotica virgifera

<400>231

aatttatatc acaagacttc cagttgggag ttgggtcaga gttaattttt taggtcttct 60

attaaaaggg ttgtaatttt aatttaaaaa aatggcatcc aatttagttg cgggagcaaa 120

tcctaaaata caggatataa tgtcgtcttt ggacgatgac aaaacagata tgttggctgc 180

tacaagtgta ttgcagcaaa gagctacaga tataagaaat caacatgtaa actggcaatc 240

ctatttccag tcacaaatga tttcgcagga agactacaac ttcatagtag cttttgatgt 300

gactgatagt gcaaaaagag aagctctcct gaaaagcgac agaaaccaat gtgctcaaac 360

attgttgaac cttttggggc atgtatccaa ggaccaaact ctgcagtaca ttttagtttt 420

ggttgatgat atgttacagg agaacagaag tcgtgtagag atatttcacg aatatgccaa 480

ccaaaagaaa gaatctgttt ggggtccatt tttgaacctt cttaaccgcc aggatggatt 540

tattaccaac atgacttcta ggatcatcgc taaaattgct tgttggtcgc agacacttat 600

ggatcgttct gaccttcatt tttacttgac ctggttaaaa gaccaactaa aaactcagaa 660

caacgaatac atccaatcgg ttgcaagatg ccttcaaatg atgctccgaa ttgacgacta 720

ccgttttgcc ttcgtgtccg tcgatggaat atccactctg ttgtcggtac tctctggaag 780

agtaaatttc caagttcagt atcagctgat attctgcttg tgggtactca ctttcaaccc 840

gttgttagcc gaaaagatga acaaattcaa cgttattcct atattggcag atattttgag 900

cgattcggta aaggagaaag taaccagaat tattttagct gttttcagga atttgatcga 960

aaaacccgaa gatcaacaag tttcaaaaga acattgtata gctatggtgc aatgcaaagt 1020

attgaaacaa ttggccattt tagaacagcg caagttcgat gatgaagatg tgacagctga 1080

tgtcgaattt ctaactgaaa aactacagag ttctgttcag gatctgagtt ccttcgatga 1140

atattcaact gaagtcaaat ctggacgttt ggaatggtct ccagtacaca agagcaagtt 1200

ctggcgggaa aatgcgcagc gcctcaacga gaaaaactac gaattactcc gtattctcat 1260

tcatttgtta gaaaccagca aggacccctt ggtattgagc gtagctagtt tcgatattgg 1320

agagtatgtt cgccattatc ctcgcggcaa acacataatc gagcagttgg gaggaaagca 1380

attagttatg caactcttag cccacgaaga tcccaatgta cgatatgaag ctctgttggc 1440

agtccaaaaa ttgatggtcc acaattggga ataccttggt cgtcaacttg aaaaagagca 1500

aagtacagat aaaaacgcg 1519

<210>232

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>232

gtttagatca agaattgaaa atcatcggag c 31

<210>233

<211>21

<212>DNA

<213>Diabrotica virgifera

<400>233

gcacctccac caccgctctt c 21

<210>234

<211>490

<212>DNA

<213>Diabrotica virgifera

<400>234

gtttagatca agaattgaaa atcatcggag cctttggtct ccgaaacaaa cgtgaagtat 60

ggagagtaaa atacactcta gccaaaatcc gtaaggccgc cagagaactg cttactctag 120

aagagaaaga acccaaaagg ttgtttgaag gtaatgcact cctacgtcgt ttggttcgta 180

ttggagtact agacgagaac agaatgaagc tcgattacgt attgggtctc aagattgaag 240

atttcttgga aagacgtctg caaacacaag tcttcaaatc tggtctagct aagtcaatcc 300

atcatgctag ggtattgatc agacaacgac acattcgggt ccgcaaacaa gttgtgaaca 360

ttccctcctt catcgtccgt ttggattcac aaaaacacat cgacttctct ctgaaatcac 420

ctttgggagg cggtcgtcca ggacgtgtta agaggaagaa cctcaccaag aagagcggtg 480

gtggaggtgc 490

<210>235

<211>1850

<212>DNA

<213>Diabrotica virgifera

<400>235

gtgaactgaa ctgtgccggt gtgatttccc ttttaaataa aagaaaagtt caaaatcgtg 60

atcgaaaaaa ccttttatta tattaataca attttttaaa atggctgcaa tgtctgtgat 120

aggtatcgat ttgggaaatg aatcctgtta tgtagctgtc gcaaaggctg gtggaatcga 180

aaccattgca aatgactaca gtttaagggc tactccatca tgcgttgctt tttctggaaa 240

gaaccgcata ttgggagttg cggcgaaaaa tcagcaagtc accaatatga agaacactgt 300

atatgggcta aaaaggctgt taggtagaaa gtttagggat cctcatgtca aaaaagaaat 360

tcagttttta ccatatacag ttgtagaaga aaaaaacggg ggtattggaa ttagggtaaa 420

ttatttaaat gaagaacata aattttcacc agaacaatgt ttggccatgt tgttaacaaa 480

attaaaagat agtgctatga cagctctaca aactcccata aatgattgtg ttatatcagt 540

tccttcttac tttacaaaca atgagaggaa ggcactatta gattctgcag ccatagcagg 600

tcttaatact ctaaggttat ttaatgaaac aacagctacg gccttgtcat atggcattta 660

caagcaggat ttaccagctg tcgaagaaaa gcctcgtaat attatttttg tcgattgtgg 720

ccattcatcc ttacaagtat ttgcagttgc atttaataag ggcaaattga agatgcttac 780

cactgctgca gatcccaatt taggtggtag ggactttgat ctcatcattg ctgagcattt 840

ttgcaaacaa ttccaaacca aatacaacat ggatgcaaaa tctaatccaa gagcttttct 900

aagattgcta acggaggtag aaaagttgaa gaagcagatg tctgcaaatt ctacaacctt 960

accactcaac atagaatgtt tcatgaacga taaagatgtg tccagtgaca tgaagagagc 1020

agatatggaa actttatgtg ctcatctgtt tgaaagagtt gaagtaagct taaagcaatg 1080

tctcaaacaa tcaggattgt cattggatga catttacgct gtagaaatag tgggcggatc 1140

tagcagagta cctgctatta aacaattaat agagaaagta ttccacaaag taccaagtac 1200

tacattaaat caagatgaag ctgtgagccg tggttgtgct ctacagtgtg ctatgctatc 1260

tcctgctata agagttcggg attttagcgt tacagatgta caggcgtacc cgatctctgt 1320

agcatgggat gcaagtccag gaggagaatc tgctggggag atagaagcat tcccacttaa 1380

ccatccggtt ccattctcga gaatgttgac attctataga caggaaccat tcaccatcag 1440

agcaaaatac agtggcaaca tcccgtatcc cgatcatgat ataggtacct gggttataaa 1500

agacataaaa cctaactctg aaggaaagcc gcagaaagtg aaagtcaagg tccgtgtaaa 1560

cctccatgga atcatgtcga tatcgagtgc tagtttgtac gaagcaaaag agtcagttga 1620

accagaaagt ccggaagaac aacagaagga ggcacaggtg aacagtaacg accaacagaa 1680

tggcggtgtg cagactaacg agagcggcga atcgatggaa acgacaaacg aaggaacagg 1740

agccaacagt ggagaaggtg ctgttcctga ggtaggctcc ggcaccagct ggacgaagaa 1800

aatatctgcc tggttcagcg gggaaggaag cgataaaaag aaaaaagcat 1850

<210>236

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>236

ctgtcgcaaa ggctggtgga atc 23

<210>237

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>237

ctcttcatgt cactggacac atctttatcg t 31

<210>238

<211>862

<212>DNA

<213>Diabrotica virgifera

<400>238

ctgtcgcaaa ggctggtgga atcgaaacca ttgcaaatga ctacagttta agggctactc 60

catcatgcgt tgctttttct ggaaagaacc gcatattggg agttgcggcg aaaaatcagc 120

aagtcaccaa tatgaagaac actgtatatg ggctaaaaag gctgttaggt agaaagttta 180

gggatcctca tgtcaaaaaa gaaattcagt ttttaccata tacagttgta gaagaaaaaa 240

acgggggtat tggaattagg gtaaattatt taaatgaaga acataaattt tcaccagaac 300

aatgtttggc catgttgtta acaaaattaa aagatagtgc tatgacagct ctacaaactc 360

ccataaatga ttgtgttata tcagttcctt cttactttac aaacaatgag aggaaggcac 420

tattagattc tgcagccata gcaggtctta atactctaag gttatttaat gaaacaacag 480

ctacggcctt gtcatatggc atttacaagc aggatttacc agctgttgaa gaaaagcctc 540

gtaatattat ttttgtcgat tgtggccatt catccttaca agtatttgca gttgcattta 600

ataagggcaa attgaagatg cttaccactg ctgcagatcc caatttaggt ggtagggact 660

ttgatctcat cattgctgag catttttgca aacaattcca aaccaaatac aacatggatg 720

caaaatctaa tccaagagct tttctaagat tgctaacgga ggtagaaaag ttgaagaagc 780

agatgtctgc aaattctaca accttaccac tcaacataga atgtttcatg aacgataaag 840

atgtgtccag tgacatgaag ag 862

<210>239

<211>1188

<212>DNA

<213>Diabrotica virgifera

<400>239

tagaatttca gacttttgac aaattaaaat tttgaacaat ttattttatt aatttcaatc 60

acattgcagt tttaaaaaga attaaaaatg gtatccctaa atcccgtaag aattctcaag 120

caagaagccg aggaagaaag ggcagagatt gcccgactca gtagttttgt aggtgctata 180

gctataggag atttggttag aagcaccttg ggaccaaaag gaatggataa aattttagta 240

tccagtggta gatctgcagg atcagttgaa gttactaacg acggagcaac tatcctaaaa 300

tcggtgggtg ttgataatcc tgctgctaaa attttggtgg atatgtcaaa agtccaggat 360

gatgaagtag gagatggcac cacatcagtg acagtattag catctgaact acttaaagaa 420

gcagaaaaac ttgtagaaca gaaaattcac ccacaaacaa tcattgccgg ttggaggaaa 480

gcagtagata ttgccagaaa agctcttcta gaaactgcca aagacaacag ctctgattca 540

gaaaagttca gagaagatct gatgaacatt gccagaacta cactcagctc aaagattctt 600

tcacaacata aagaatattt tgccaaactg gcagtagatg ctgtgctgag attgaaaggt 660

acaggtgatt tacaatccat ccagctgatt aagaaaactg gtggtacttt ggaagattcc 720

tttttggatg aaggtttttt attggacaaa aaacctggtg tacatcaacc aaagagagta 780

gaaaatgcaa aaatcctgat agccaacact cctatggaca cagataaaat caaagtattt 840

ggttctcaca ttaaggtaga ttcaatggct aaaattgcag agctggaatc tgcagaaaag 900

gaaaaaatga aagataaagt gaacaaaatt cttaaacata atgctaatgt atttattaat 960

agacagttga tttacaatta tcctgaacaa ttgtttgctg atgcaggagt tatggctatt 1020

gaacatgcag attttgatgg aattgaaagg ctagcacttg tcacaggagg agagattgtc 1080

tcaacatttg accatccaga attggtaaaa cttggaactt gtaagcttat tgaacaaatc 1140

atgattggag aagatatcct gctccgtttc agtggtgttg ctcttggg 1188

<210>240

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>240

ggtgggtgtt gataatcctg ctgct 25

<210>241

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>241

caagagcaac accactgaaa cggag 25

<210>242

<211>884

<212>DNA

<213>Diabrotica virgifera

<400>242

ggtgggtgtt gataatcctg ctgctaaaat tttggtggat atgtcaaaag tccaggatga 60

tgaagtagga gatggcacca catcagtgac agtattagca tctgaactac ttaaagaagc 120

agaaaaacct gtagaacaga aaattcaccc acaaacaatc attgccggtt ggaggaaagc 180

agtagatatt gccagaaaag ctcttctaga aactgccaaa gacaacagct ctgattcaga 240

aaagttcaga gaagatctga tgaacattgc cagaactaca ctcagctcaa agattctttc 300

acaacataaa gaatattttg ccaaactggc agtagatgct gtgctgagat tgaaaggtac 360

aggtgattta caatccatcc agctgattaa gaaaactggt ggtactttgg aagattcctt 420

tttggatgaa ggttttttat tggacaaaaa acctggtgta catcaaccaa agagagtaga 480

aaatgcaaaa atcctgatag ccaacactcc tatggacaca gataaaatca aagtatttgg 540

ttctcacatt aaggtagatt caatggctaa aattgcagag ctggaatctg cagaaaagga 600

aaaaatgaaa gataaagtga acaaaattct taaacataat gctaatgtat ttattaatag 660

acagttgatt tacaattatc ctgaacaatt gtttgctgat gcaggagtta tggctattga 720

acatgcagat tttgatggaa ttgaaaggct agcacttgtc acaggaggag agattgtctc 780

aacatttgac catccagaat tggtaaaact tggaacttgt aagcttattg aacaaatcat 840

gattggagaa gatatcctgc tccgtttcag tggtgttgct cttg 884

<210>243

<211>1188

<212>DNA

<213>Diabrotica virgifera

<400>243

tagaatttca gacttttgac aaattaaaat tttgaacaat ttattttatt aatttcaatc 60

acattgcagt tttaaaaaga attaaaaatg gtatccctaa atcccgtaag aattctcaag 120

caagaagccg aggaagaaag ggcagagatt gcccgactca gtagttttgt aggtgctata 180

gctataggag atttggttag aagcaccttg ggaccaaaag gaatggataa aattttagta 240

tccagtggta gatctgcagg atcagttgaa gttactaacg acggagcaac tatcctaaaa 300

tcggtgggtg ttgataatcc tgctgctaaa attttggtgg atatgtcaaa agtccaggat 360

gatgaagtag gagatggcac cacatcagtg acagtattag catctgaact acttaaagaa 420

gcagaaaaac ttgtagaaca gaaaattcac ccacaaacaa tcattgccgg ttggaggaaa 480

gcagtagata ttgccagaaa agctcttcta gaaactgcca aagacaacag ctctgattca 540

gaaaagttca gagaagatct gatgaacatt gccagaacta cactcagctc aaagattctt 600

tcacaacata aagaatattt tgccaaactg gcagtagatg ctgtgctgag attgaaaggt 660

acaggtgatt tacaatccat ccagctgatt aagaaaactg gtggtacttt ggaagattcc 720

tttttggatg aaggtttttt attggacaaa aaacctggtg tacatcaacc aaagagagta 780

gaaaatgcaa aaatcctgat agccaacact cctatggaca cagataaaat caaagtattt 840

ggttctcaca ttaaggtaga ttcaatggct aaaattgcag agctggaatc tgcagaaaag 900

gaaaaaatga aagataaagt gaacaaaatt cttaaacata atgctaatgt atttattaat 960

agacagttga tttacaatta tcctgaacaa ttgtttgctg atgcaggagt tatggctatt 1020

gaacatgcag attttgatgg aattgaaagg ctagcacttg tcacaggagg agagattgtc 1080

tcaacatttg accatccaga attggtaaaa cttggaactt gtaagcttat tgaacaaatc 1140

atgattggag aagatatcct gctccgtttc agtggtgttg ctcttggg 1188

<210>244

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>244

ctcactctga tggtgtaatc actatggaaa tc 32

<210>245

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>245

gtcttctaca aatgctgatt tcaaatggtc t 31

<210>246

<211>820

<212>DNA

<213>Diabrotica virgifera

<400>246

ctcactctga tggtgtaatc actatggaaa tcgtgggatg cagaccttcc gattctggaa 60

aatactcctg cgttgctaca aacgaacacg gcgtagacga aacatcttgt gtagttattg 120

tagaaggaca aaccagcaca ccagaacaac aagaactagc caacaaatta ctctattcag 180

gagacaggaa gtacattgaa catcctataa agccagctcc aattcctgta actgtgcgaa 240

aaccaatacc ctcatcaaat cctcaccctc tacaaccgaa atctaacaat cctagcacaa 300

atagtttagc gcattcttca tccacactca gtgttggaga tggagacaag aggacgccta 360

ggaaatatgg tagattagat tctactggaa gtccaaatag atcaagaagc gcaactaaag 420

aattagcact gcctgatgac tcaacaatgt acgccccaca atttaccaaa aatctcacag 480

acttaaccat caatgatggt gaaagtctaa ccttgactgc acacgtaaag ggagatccag 540

atcctcaaat tgtttggacc aaaaatggaa aagccttgag ttcctctgaa gttgttgacc 600

tcaaatacaa gaacggaata gcgaaattac acataaatga agtataccct gaagatgaag 660

gagaatatgt gtgcagagca accaactctg taggaacctc tgagaccaaa tgcaaactaa 720

ccattaaacc aatggccaat gccaccaacg gcaagaaaaa atcctcagaa gacaaacccc 780

caagaatagt agaccattga aatcagcatt tgtagaagac 820

<210>247

<211>815

<212>DNA

<213>Diabrotica virgifera

<400>247

gttataccag aaaaagaaga aggtagtcct tcggaagtaa aacaagtgct gcaggatgtg 60

gatactgaag atttgtacac taaatataaa aaacttcaac aacagttgga gttcttggag 120

gttcaagaag agtacattaa agatgagcaa aggaatctca aaaaggagta tttacatgct 180

caggaagaag tgaagcgtat ccagtctgtc ccgcttgtta ttggacagtt tttagaagct 240

gtagatcaaa acactggcat tgttggctca actacaggtt caaattatta tgttagaatc 300

ttatcaacaa ttgatagaga actcttaaaa ccttctgcta gtgttgctct tcataaacac 360

agcaatgcct tagtagatgt cctaccacca gaggcagatt cttcaataag catgctccag 420

gctgatgaaa aaccagatgt tcaatacagt gacattggag gtatggatat gcagaaacaa 480

gaaattaggg aagctgtaga gttacctttg actcacttcg aattgtataa acagattggt 540

attgatcccc ccagaggtgt gttgatgtat ggacctcctg gatgcgggaa gactatgttg 600

gccaaagctg ttgctcatca tactacagct gcctttattc gtgtagtcgg atcagaattc 660

gtacagaaat atcttggtga aggtcctcga atggtgagag acgtgttccg tctagcaaaa 720

gaaaattctc cagcaatcat ctttattgat gaaattgatg caatcgctac caaacgtttt 780

gatgcccaga caggtgctga tcgtgaagtc cagag 815

<210>248

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>248

cttcaacaac agttggagtt cttggagg 28

<210>249

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>249

ctctggactt cacgatcagc acctg 25

<210>250

<211>722

<212>DNA

<213>Diabrotica virgifera

<400>250

cttcaacaac agttggagtt cttggaggtt caagaagagt acattaaaga tgagcaaagg 60

aatctcaaaa aggagtattt acatgctcag gaagaagtga agcgtatcca gtctgtcccg 120

cttgttattg gacagttttt agaagctgta gatcaaaaca ctggcattgt tggctcaact 180

acaggttcaa attattatgt tagaatctta tcaacaattg atagagaact cttaaaacct 240

tctgctagtg ttgctcttca taaacacagc aatgccttag tagatgtcct accaccagag 300

gcagattctt caataagcat gctccaggct gatgaaaaac cagatgttca atacagtgac 360

attggaggta tggatatgca gaaacaagaa attagggaag ctgtagagtt acctttgact 420

cacttcgaat tgtataaaca gattggtatt gatcccccca gaggtgtgtt gatgtatgga 480

cctcctggat gcgggaagac tatgttggcc aaagctgttg ctcatcatac tacagctgcc 540

tttattcgtg tagtcggatc agaattcgta cagaaatatc ttggtgaagg tcctcgaatg 600

gttagagacg tgttccgtct agcaaaagaa aattctccag caatcatctt tattgatgaa 660

attgatgcaa tcgctaccaa acgttttgat gcccagacag gtgctgatcg tgaagtccag 720

ag 722

<210>251

<211>1324

<212>DNA

<213>Diabrotica virgifera

<400>251

tttaaaatta aaatataaat agaagagaat tgtgtttgta taattgttta aaattaaaat 60

aatttaataa tggaaaacga aggcgaagct gtaacagaac agaagcgtct gttgtgttgt 120

tttaaatcgg aaaatggaga agtcgccggt gatatgatgg atctccccat agactgttcc 180

ttacagcagc tcacgttaat ttgtaatgct ttgttaaaac aggaagaacc agtaccatac 240

ttattttacg ttaacgaaaa cgaaataacc catactttgg aaaaagcact aaatataagg 300

gaattaaata cagaaggtgt tgtagatatc atttatcaac aacaagcagt gtttagggta 360

cgccctgtaa caaggtgtac cagctcaata ccaggtcatg cagaagcagt tatttcagtt 420

agttttagtc cagacggtaa acatttagca agtggatctg gtgatacaac agtcaggttt 480

tgggatgttg atacgcaatc tccgttatat acttgtgaag gtcataaaaa ttgggtgttg 540

tgtatagctt ggtcctctga tagtactaaa ttggcttctg cttgtaaaga tgggaaaatt 600

atcatatggg atccttttac aggaaagcaa ataggtaaaa cgttaacggg acataaacag 660

tggattacta gtttaagctc gggagcctta tcataaaaac cctgaatgta gatatttggc 720

aagttcttca aaagatggag atgtaaggat atgggatact gtactcagca atacattttt 780

ttgtatatct ggtcatacaa agagtgtaac aatggtgaaa tggggtggag caggtctcat 840

ctattcagcc tcccaagaca gaacagttaa agtatggaga gctaaagatg gtgtactatg 900

taggacatta gaaggacatg cccattgggt taatacatta gctttaaata ctgactacat 960

tttaaggata ggagcatttg atcccgttaa agattcaaat aaagacacat taatgccaga 1020

caagaaaaaa tctcaggact ttgccctcaa acgatactat gaagttacag aatctattgg 1080

agaaagatta gtatcgggtt ctgatgattt cacattattt ctttggaatc ctgataaaga 1140

caagaagcct ttagctaggc taacaggtca ccaacaatta gtcaatgacg tgaaattttc 1200

acctgatggt agaatattag cttcagcatc tttcgataaa tcaatcaaat tgtgggaggc 1260

aaaacaggaa agtttatttg taccctacgt ggacatgttc aggctgtgta catggtatca 1320

tttt 1324

<210>252

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>252

gaaaacgaag gcgaagctgt aacagaac 28

<210>253

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>253

gctccacccc atttcaccat tgttacac 28

<210>254

<211>757

<212>DNA

<213>Diabrotica virgifera

<400>254

gaaaacgaag gcgaagctga acagaacaga agcgtctgtt gtgttgtttt aaatcggaaa 60

atggagaagt cgccggtgat atgatggatc tccccataga ctgttcctta cagcagctca 120

cgttaatttg taatgctttg ttaaaacagg aagaaccagt accatactta ttttacgtta 180

acgaaaacga aataacccat actttggaaa aagcactaaa tataagggaa ttaaatacag 240

aaggtgttgt agatatcatt tatcaacaac aagcagtgtt tagggtacgc cctgtaacaa 300

ggtgtaccag ctcaatacca ggtcatgcag aagcagttat ttcagttagt tttagtccag 360

acggtaaaca tttagcaagt ggatctggtg atacaacagt caggttttgg gatgttgata 420

cgcaatctcc gttatatact tgtgaaggtc ataaaaattg ggtgttgtgt atagcttggt 480

cccctgatag tactaaattg gcttctgctt gtaaagatgg gaaaattatc atatgggatc 540

cttttacagg aaagcaaata ggtaaaacgt taacgggaca taaacagtgg attactagtt 600

taagctggga gccttatcat aaaaaccctg aatgtagata tttggcaagt tcttcaaagg 660

atggagatgt aaggatatgg gatactgtcc tcagcaacac atttttttgt atatctggtc 720

atacaaagag tgtaacaatg gtgaaatggg gtggagc 757

<210>255

<211>794

<212>DNA

<213>Diabrotica virgifera

<400>255

caaattatca acaaaacaga cgccattttg ctaaaattga aatatccaac aaagtagaaa 60

gagattttaa ataaatctaa aacatccaaa cttttttttg ggaacgtatc ggaagtaatg 120

tgtctgtgat aaatagtaaa aaatattaaa gcgatatttt cgtcgaaact gatatgtatt 180

aaatttatct tttgtgttat taaagagagt taaacgtagc taaagaagaa aaactattag 240

tgatttaaac atgagtacga aagatgatga atacgattat ttatttaaag ttgtccttat 300

tggtgattct ggtgtaggaa aaagtaactt attgtcaaga tttactagaa atgaattcaa 360

tttggagtct aaatctacta taggtgtcga attcgctacc agaagtatac aggtggatgg 420

taaaactatt aaagcacaaa tatgggacac ggcaggtcaa gagagatacc gagcaatcac 480

ggcggcctat tatcgtggtg cagtcggtgc tctactcgtc tacgatatcg ccaaacatct 540

gacatacgaa aacgttgaac gttggttgcg cgaattgcgc gaccacgcag atcagaacat 600

tgtgatcatg ttggtcggaa acaaatccga tctgaggcat ttgcgcgccg tgctgacaga 660

agaagctaag gctttcgccg aaagaaacgg cctcagcttc atcgagactt cagccctaga 720

ttcgaccaat gtcgataatg ctttccaaaa tatactcaca gagatctata gaattgtttc 780

ccaaaaacag atca 794

<210>256

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>256

ggaacgtatc ggaagtaatg tgtctgtg 28

<210>257

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>257

cacaatgttc tgatctgcgt ggtcg 25

<210>258

<211>504

<212>DNA

<213>Diabrotica virgifera

<400>258

ggaacgtatc ggaagtaatg tgtctgtgat aaatagtaaa aaatattaaa gcgatatttt 60

cgtcgaaact gatatgtatt aaatttatct tttgtgttat taaagagagt taaacgtagc 120

taaagaagaa aaactattag tgatttaaac atgagtacga aagatgatga atacgattat 180

ttatttaaag ttgtccttat tggtgattct ggtgtaggaa aaagtaactt attgtcaaga 240

tttactagaa atgaattcaa tttggagtct aaatctacta taggtgtcga attcgctacc 300

agaagtatac aggtggatgg taaaactatt aaagcacaaa tatgggacac ggcaggtcaa 360

gagagatacc gagcaatcac ggcggcctat tatcgtggtg cagtcggtgc tctactcgtc 420

tacgatatcg ccaaacatct gacatacgaa aacgttgaac gttggttgcg cgaattgcgc 480

gaccacgcag atcagaacat tgtg 504

<210>259

<211>1180

<212>DNA

<213>Diabrotica virgifera

<400>259

atttttgggt gtttctttga ttgaacaaaa attacaagcg ttttaaattc actaaatttg 60

ctttgaaata tacaaatagt taattctagg aaaatatata acctacatac agaatcatgt 120

cttcccgcaa aaccgtaagc cgccgtggta ccacaaagaa acgtgcccaa agagccacat 180

ccaatgtatt cgcaatgttt gatcaggctc agattgctga gttcaaagaa gccttcaata 240

tgatcgacca gaaccatgat ggttttgtag ataaagaaga tctgcatgat atgcttgctt 300

ccttaggtaa aaacccaact gatgactatc tagatggcat gatgaatgaa gctcctggtc 360

ccataaactt caccatgttc ttaactctct ttggagaacg tctacaaggc acagacccag 420

aagacgtaat taaaaacgct tttggctgct ttgacgaaga caacaatggt gtcctcaacg 480

aagaacgtct cagggagctc ctcacgtcca tgggtgatag attcactgac gatgaagttg 540

acgagatgta cagggaggct cctattaaga atggcttgtt tgattatgtt gaattcaccc 600

gcattttaaa gcacggggct aaggacaagg atgagcaata agagacgaat tgatgatgaa 660

aggatttgta attaatatgg aaagatgtct tagtttgaat gatttggatt ttcattccat 720

ttttgtgatt agaaatatat gaaattcatc atactagacc atcaagtctt gtccgttgca 780

gtatcttctg cacatagaca tgtcatcctt gaacaaaact gcaaaaatgg atttgaaaaa 840

tggtaggttt tgaatgaatt aacagtctaa ttatatagac atcttgttat tgtacttaac 900

atctcttgct tgttatttta aagtattcct tgaatgtttt gaatagaaaa cgacattttg 960

tatattaaaa ctacaaatcc aattgcatgt tatcattata agatcgatat ttttccttca 1020

aagtattttt agcattgtta gtcagcttat tttgggtttt tacaactttt ggcaacgttt 1080

tatttattca taagatttat tgtaattata aattttatac ctttatatat catgtaatta 1140

ttcattaaaa tattgaacca aaaaaaaacc cacgcgtccg 1180

<210>260

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>260

gagccacatc caatgtattc gcaatg 26

<210>261

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>261

caacggacaa gacttgatgg tctagtatga 30

<210>262

<211>607

<212>DNA

<213>Diabrotica virgifera

<400>262

gagccacatc caatgtattc gcaatgtttg atcaggctca gattgctgag ttcaaagaag 60

ccttcaatat gatcgaccag aaccatgatg gttttgtaga taaagaagat ctgcatgata 120

tgcttgcttc cttaggtaaa aacccaactg atgactatct agatggcatg atgaatgaag 180

ctcctggtcc cataaacttc accatgttct taactctctt tggagaacgt ctacaaggca 240

cagacccaga agacgtaatt aaaaacgctt ttggctgctt tgacgaagac aacaatggtg 300

tcctcaacga agaacgtctc agggagctcc tcacgtccat gggtgataga ttcactgacg 360

atgaagttga cgagatgtac agggaggctc ctattaagaa tggcttgttt gattatgttg 420

aattcacccg cattttaaag cacggggcta aggacaagga tgagcaataa gagacgagtt 480

gatgatgaaa ggatttgtaa ttaatatgga acgatgtctt agtttgaatg atttggattt 540

tcattccatt tttgtgatta gaaatatatg aaattcatca tactagacca tcaagtcttg 600

tccgttg 607

<210>263

<211>966

<212>DNA

<213>Diabrotica virgifera

<400>263

gatctagtga ccagccttga agctatttct acacagagtg gtatagtgtc tggtgtagtt 60

gataatctca caagagccat gactcgattg tcggatagtc gtgcttctct catcttatct 120

gatggtgata ttggattcgt tgattaccaa acacgaatgg tggactgtgc gaagagtata 180

gctaaaatcg ccggagaaat tgctagcaaa gctgccactg accctcagaa ggtggctcaa 240

ttatctgcag atcttgcgca caaatatgca caactatctg gcgatagtat tggagctgca 300

gcagctgcca ctaatgccga agttgcaatc agactaaggg aagttgttca acaactgggt 360

ggcgcctgcg tagaactcgt ccaaacagga ggccagtgct tagtccgaaa agatgacgta 420

ctcttgcgag aagtaggaga ttgctcacgc aacgtaactg agaaagtttc ccgtgttctt 480

gccacattac aaaacgaatc cagaggtacc caagcttgca tcaatgcagc ttccaccgta 540

tctggtatca tcggcgattt agaaactacc atcatgtttg ccactgccgg aaatctcaat 600

cccgaaagcg aacatgaatc gttcgctaat cacagagaga atatattgaa gactgctaag 660

gcgttggtag aggatactaa gactttggta gctggcgcag cttcatcaca agaacaactg 720

gctgtagctg ctcaaaattc tgttgcgacc atagtacaat tagccgaagt agtgaaattc 780

ggagcagcca gcttaggcgt tgacaatcca gattcccaag tgatgcttat caacgcagta 840

cgtgacgttg ccagtgctct tggtgacttg attcaagcta ctaaggctgc tagcggcaag 900

cccatcaacg atccggccat ggctcattta aaagacagcg caaaggtgac ggattctgaa 960

actgat 966

<210>264

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>264

ggtgtagttg ataatctcac aagagccatg 30

<210>265

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>265

cagtttcaga atccgtcacc tttgcg 26

<210>266

<211>913

<212>DNA

<213>Diabrotica virgifera

<400>266

ggtgtagttg ataatctcac aagagccatg actcgattgt cggatagtcg tgcttctctc 60

atcttatctg atggtgatat tggattcgtt gattaccaaa cacgaatggt ggactgtgcg 120

aagagtatag ctaaaatcgc cggagaaatt gctagcaaag ctgccactga ccctcagaag 180

gtggctcaat tatctgcaga tcttgcgcac aaatatgcac aactatctgg cgatagtatt 240

ggagctgcag cagctgccac taatgccgaa gttgcaatca gactaaggga agttgttcaa 300

caactgggtg gcgcctgcgt agaactcgtc caaacaggag gccagtgctt agtccgaaaa 360

gatgacgtac tcttgcgaga agtaggagat tgctcacgca acgtaactga gaaagtttcc 420

cgtgttcttg ccacattaca aaacgaatcc agaggtaccc aagcttgcat caatgcagct 480

tccaccgtat ctggtatcat cggcgattta gaaactacca tcatgtttgc cactgccgga 540

aatctcaatc ccgaaagcga acatgaatcg ttcgctaatc acagagagaa tatattgaag 600

actgctaagg cgttggtaga ggatactaag actttggtag ctggcgcagc ttcatcacaa 660

gaacaactgg ctgtagctgc tcaaaattct gttgcgacca tagtacaatt agccgaagta 720

gtgaaattcg gagcagccag cttaggcgtt gacaatccag attcccaagt gatgcttatc 780

aacgcagtac gtgacgttgc cagtgctctt ggtgacttga ttcaagctac taaggctgct 840

agcggcaagc ccatcaacga tccggccatg gctcatttaa aagacagcgc aaaggtgacg 900

gattctgaaa ctg 913

<210>267

<211>846

<212>DNA

<213>Diabrotica virgifera

<400>267

ctctctttga gttgtcgctt ttcgaggtat ttagttgcag gcgtggtgta tacgttgaaa 60

tagtgatttt tgttaaaagt gagtaaatat ggcatcgaaa tcaaaaaaag ggtcaacacc 120

gttagcttca cagcggccgg cgagcccctt aagtcctacc agacattcta gattacagga 180

gaagcatgaa cttcagaatc ttaacgaccg tttggcggga tatatagatc gagtaaggta 240

tttagaaggt gaaaacaaca gattaacgag ggaaatacaa agtacccagg aaacagtaac 300

aagggaagtt tcaaacatta aatctatgta tgaccatgaa ttatcagatg ctagaaaact 360

attagacgag acccaccgtg aaaaagcacg tttagagata gatgttaaac gtcttttaga 420

agagaatgac gaactcaagt ctgagttagc aaagaagaac aaggatttgg ctctagctga 480

gaattctgcc aggatatacg agaccagatg taacgagtta cagctcaaag caaatcaagc 540

ggctgctgat gctaagaaag ctattgcaga tgctaaggaa cttgagaaag aacgtgataa 600

gctacgcaaa cttcttgata atcatcgtaa acagttagaa gaagaatcat tagcgagagt 660

ggaagtcgaa aatgccaacc aaagtcttcg agaagagctg tcctttaagg atcagatcca 720

tcaacaacag ttgacagaaa ctcgtactcg tcgtcaagta gaaatcagcg aaatcgacgg 780

tcgcctggca gaaaaatatg aggctaagct tcaagaggct ctacaagaac tcagagatca 840

atatga 846

<210>268

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>268

gagaagcatg aacttcagaa tcttaacgac c 31

<210>269

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>269

gatttcgctg atttctactt gacgacgag 29

<210>270

<211>595

<212>DNA

<213>Diabrotica virgifera

<400>270

gagaagcatg aacttcagat cttaacgacc gtttggcggg atatatagat cgagtaaggt 60

atttagaagg tgaaaacaac agattaacga gggaaacaca aagtacccag gaaacagtaa 120

caagggaagt ttcaaacatt aaatctatgt atgaccatga attatcagat gctagaaaac 180

tattagacga gacccaccgt gaaaaagcac gtttagagat agatgttaaa cgtcttttag 240

aagagaatga cgaactcaag tctgagttag caaagaagaa caaggatttg gctctagctg 300

agaattctgc caggatatac gagaccagat gtaacgagtt acagctcaaa gcaaatcaag 360

cggctgctga tgctaagaaa gctattgcag atgctaagga acttgagaaa gaacgtgata 420

agctacgcaa acttcttgat aatcatcgta aacagttaga agaagaatca ttggcgagag 480

tggaagtcga aaatgccaac caaagtcttc gagaagagct gtcctttaag gatcagatcc 540

atcaacaaca gttgacagaa actcgtactc gtcgtcaagt agaaatcagc gaatc 595

<210>271

<211>901

<212>DNA

<213>Diabrotica virgifera

<400>271

tcaacacgtg tgaattaaaa caaattcaaa aacaaaaatg tccatccaag aaaaagtttt 60

aatgaaaaaa ataaaaaaga gggaaaagca gaaattaaaa tttgttcaaa agaaagagtc 120

tgaaaagagc aaagaagttt tagaaacaga tctaccagag aaggcaaaag tgggaaatgg 180

cttgaaacgc ttgcagagca acgatgaagg accttctaaa aaaaaaaaga agaaaaataa 240

aaagaagaaa gagaaaatag tcgaagagga agatacagac gaagaacaac cagaggaaga 300

tgaaattgag tctgacattg aagatcaaga gattgacaat ggtgaagaag atacagatga 360

aaaatctgat ttaggaaaca aattaccagg atccagtgct acattagaaa ttctcagtaa 420

aagtacattt gactcattga cggataaagt atgtgaaaat actttgaaag ccattgcaga 480

tatgggtttt actactatga ctgaaattca agcaaaatct atacctccac ttctggaagg 540

tagagatttg gttggggctg caaaaacagg atctggaaaa actcttgctt ttctcattcc 600

cgctgttgaa cttatttata agttaaagtt tatgccaaga aatggtactg gtatcattat 660

catttccccc acacgagaat tatcaatgca gacttttgga gtattaaaag aactaatgaa 720

atatcatcac catacatacg gtttggttat gggtggaact gcaagacaaa cagaagcaca 780

aaagctttcc aaaggtatta atatcttagt agcaacgcct ggtaggcttc tggatcatct 840

tcagaatact cctgacttct tgtataagaa tttacaatgt ttagtcattg acgaggctga 900

c 901

<210>272

<211>22

<212>DNA

<213>Diabrotica virgifera

<400>272

ggaaatggct tgaaacgctt gc 22

<210>273

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>273

ctgaagatga tccagaagcc taccagg 27

<210>274

<211>672

<212>DNA

<213>Diabrotica virgifera

<400>274

ggaaatggct tgaaacgctt gcagagcaac gatgaaggac cttctaaaaa aaaaaagaag 60

aaaaataaaa agaagaaaga gaaaatagtc gaagaggaag atacagacga agaacaacca 120

gaggaagatg aaattgagtc tgacattgaa gatcaagaga ttgacaatgg tgaagaagat 180

acagatgaaa aatctgattt aggaaacaaa ttaccaggat ccagtgctac attagaaatt 240

ctcagtaaaa gtacatttga ctcattgacg gataaagtat gtgaaaatac tttgaaagcc 300

attgcagata tgggttttac tactatgact gaaattcaag caaaatctat acctccactt 360

ctggaaggta gagatttggt tggggctgca aaaacaggat ctggaaaaac tcttgctttt 420

ctcattcccg ctgttgaact tatttataag ttaaagttta tgccaagaaa tggtactggt 480

atcattatca tttcccccac acgagaatta tcaatgcaga cttttggagt attaaaagaa 540

ctaatgaaat atcatcacca tacatacggt ttggttatgg gtggaactgc aagacaaaca 600

gaagcacaaa agctttccaa aggtattaat atcttagtag caacgcctgg taggcttctg 660

gatcatcttc ag 672

<210>275

<211>512

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(370)..(452)

＜223〉n=a, c, g or t/u

<400>275

atttagtcca ctgtacaaat gaaccaaatg tttcaattcc acagctagca aatttgttaa 60

tagaaagatc acagaataca aactgggtag tagtttacaa ggcattagtt acagttcacc 120

acatgatgtg ttatggaaat gagagattta ctcagtacct agcatccagt aacagtacat 180

ttcaacttag caacttctta gataaagggg gacttcaagg atatgacatg tctccattta 240

ttagaaaata ttcacgatat ctcaatgaga aggccttatc atacagaact acagccttcg 300

acttttgcaa agttaaaaga gggaaggatt gatggcatgt taaggacaat gaacgcagat 360

aaattattan aaactcttcc cactttacaa natcaactgg atatcctatt ggaantttga 420

tgttccgcan atgatttnna caacggagtg antaatatgt gtttcatgtt gctttttagg 480

gatttatacg cttatttttg ctgtataatg at 512

<210>276

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>276

caaatgaacc aaatgtttca attccaca 28

<210>277

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>277

cgtataaatc cctaaaaagc aacatgaaac ac 32

<210>278

<211>470

<212>DNA

<213>Diabrotica virgifera

<400>278

tgaaccaaat gtttcaattc cacagctagc aaatttgtta atagaaagat cacagaatac 60

aaactgggta gtagtttaca aggcattagt tacagttcac cacatgatgt gttatggaaa 120

tgagagattt actcagtacc tagcatccag taacagtaca tttcaactta gcaacttctt 180

agataaaggg ggacttcaag gatatgacat gtctccattt attagaaaat attcacgata 240

tctcaatgag aaggccttat catacagaac tacagccttc gacttttgca aagttaaaag 300

agggaaggat gatggcatgt taaggacaat gaacgcagat aaattattaa aaactcttcc 360

cactttacaa aatcaactgg atatcctatt ggaatttgat tgttccgcaa atgatttaac 420

aaacggagtg ataaatatgt gtttcatgtt gctttttagg gatttatacg 470

<210>279

<211>978

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(918)

＜223〉n=a, c, g or t/u

<400>279

cgcgtgggcg cgaacaggcg agaaaagacc tcaaaggact cgaagacact gtcgccaaag 60

aactacagac tctgcacaac ctgagaaaat tgttcgtgca agatttgcag gcacgtatga 120

agaaaaatct ttcgtcggaa gacaatgagg aagacggcgg ctctctggct caaaaacaga 180

agatatcgtt cctcgaaaac aatctcgatc aattgacaaa agtccacaag cagttggtac 240

gagacaacgc ggatctgcgc tgtgaacttc ccaaactcga aaagagacta cgagctacga 300

tggaaagagt caaagcactg gaaactgcac tgaaggaagc caaagaaggc gcgatgagag 360

accggaaaag ataccaatac gaggttgacc gcattaaaga ggctgtgagg cagaagaacc 420

tcgcaagaag aggcccagca gccacaatcg ctaaaccaat cagggcaggg caacacaaca 480

cggttaacgt agctggtaac cccgtcatta gaactggagg catcaacatg gccaacagat 540

cgaccgacga ccagaaacgt aggtctgtta ttggtggaga taaagattga gtcgccagcc 600

atcttgtcag gtgcttgaga cgtttttagt aagcaaaatc cataaaactg taataccaaa 660

aatattgccg atacttcgcg cgagtctgtc gccagcagtt tttggaacag acaagaaaaa 720

aaattgctcc tccagactcg ctcttcaatg tgcttacagc ttagatattt ctgtagtata 780

aggtgcactt cattgcattg aaaatgaaac aaattaatcg agtattgtaa cagattgttg 840

gcaaattgtt ttaataaatc gttcattttt tgttttcgat cacttctagc ttcctgtcga 900

attaagtgtt aacactanaa aacaatttgc ttgctgtttt atccttaaaa cgttgcaacg 960

tttacggcga ttttttga 978

<210>280

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>280

gaacaggcga gaaaagacct caaagg 26

<210>281

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>281

cacattgaag agcgagtctg gaggag 26

<210>282

<211>741

<212>DNA

<213>Diabrotica virgifera

<400>282

gaacaggcga gaaaagacct caaaggactc gaagacactg tcgccaaaga actacagact 60

ctgcacaacc tgagaaaatt gttcgtgcaa gatttgcagg cacgtatgaa gaaaaatctt 120

tcgtcggaag acaatgagga agacggcggc tctctggctc aaaaacagaa gatatcgttc 180

ctcgaaaaca atctcgatca attgacaaaa gtccacaagc agttggtacg agacaacgcg 240

gatctgcgct gtgaacttcc caaactcgaa aagagactac gagctacgat ggaaagagtc 300

aaagcactgg aaactgcacc gaaggaagcc aaagaaggcg cgatgagaga ccggaaaaga 360

taccaatacg aggttgaccg cattaaagag gctgtgaggc agaagaacct cgcaagaaga 420

ggcccagcag ccacaatcgc caaaccaatc agggcagggc aacacaacac ggttaacgta 480

gctggtaacc ccgtcattag aactggaggc atcaacgtgg ccaacagatc gaccgacgac 540

cagaaacgta ggtctgttat tggtggagat aaagattgag tcgccagcca tcttgtcagg 600

tgcttgagac gtttttagta agcaaaatcc ataaaactgt aataccaaaa atattgccga 660

tacttcgcgc gagtctgtcg ccagcagttt ttggaacaga caagaaaaaa aattgctcct 720

ccagactcgc tcttcaatgt g 741

<210>283

<211>876

<212>DNA

<213>Diabrotica virgifera

<400>283

ggaaaactgg tcccttctgt ttttctgatg tctcaaattt tctgtcctaa cttctaacac 60

ttgtttaaaa tccgttgaca tacttgctaa tttggatgga gtgctaagac aatgctactc 120

gtgtgtgatt gtagatgttt cccacttgat ccctgcttct gtttcctaga tacatcttgt 180

aattgggcta tttgttgatt caagttgcta agatcacctt ttatgatata tgtgagttct 240

tggatttctg atgttttgtc gtcaaataaa gattttcgtt ttgccaacaa tgtcaacttt 300

tccaatttag cataagtgct agctatatgc cgtcccacat tcttcgcaat tagcataaac 360

tcgctatgac tttgtataac cctcgatttc ttcgggtccc tagcagctac tgccctagca 420

atgttcctgc cttgtaatgt ctgtatagta tttacaaatt cactagttct gtctctggca 480

gtcatgacta attctggttc agtgtagtct tcgatataat cttcctctaa atagtagtca 540

gatgtaacat tgtttggata caccgtgtgt gactgattcg ttttaggctg agggttagtc 600

caataattat cgttacctac tataacttgg ggctcagttt cagagccagc tcgacgacgt 660

ctaggaagca ttgcattctt aagaggctac aaagttaatt ttgaaagtag cacaaccata 720

tttaaagcac tagaatttca ctttatatta tatttttatc acctactgta gtatatatca 780

tgactaattg tttaattggg tttaatatgt ttaattacaa ttaataatga actttacttt 840

ttcttttgaa agtacacgtc tatttgcccg gacgcg 876

<210>284

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>284

ggttgtgcta ctttcaaaat taactttgta gc 32

<210>285

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>285

cgttgacata cttgctaatt tggatggag 29

<210>286

<211>645

<212>DNA

<213>Diabrotica virgifera

<400>286

ggttgtgcta ctttcaaaat taactttgta gcctcttaag aatgcaatgc ttcctagacg 60

tcgtcgagct ggctctgaaa ctgagcccca agttatagta ggtaacgata attattggac 120

taaccctcag cctaaaacga atcagtcaca cacggtgtat ccaaacaatg ttacatctga 180

ctactattta gaggaagatt atatcgaaga ctacactgaa ccagaattag tcatgactgc 240

cagagacaga actagtgaat ttgtaaatac tatacagaca ttacaaggca ggaacattgc 300

tagggcagta gctgctaggg acccgaagaa atcgagggtt atacaaagtc atagcgagtt 360

tatgctaatt gcgaagaatg tgggacggca tatagctagc acttatgcta aattggaaaa 420

gttgacattg ttggcaaaac gaaaatcttt atttgacgac aaaacatcag aaatccaaga 480

actcacatat atcataaaag gtgatcttag caacttgaat caacaaatag cccaattaca 540

agatgtatct aggaaacaga agcagggacc aagtgggaaa catctacaat cacacacgag 600

tagcattgtc ttagcactcc atccaaatta gcaagtatgt caacg 645

<210>287

<211>883

<212>DNA

<213>Diabrotica virgifera

<400>287

cccgttacat cattttatca tatataaaat gattttaatt tgtttataat gtttattaag 60

taaaagcatt tcgccatcgt aacatgtcta agatttttgc gttatacggg gaactatgtg 120

ccactcaccc atgggaagtt atttttactg tcatcacctt cactgtatgt tttttgactt 180

tggataaacc gcaagggaca aaggcctttc cacggactac ctcaacatgt agcacctgca 240

ccgaagacca ttttatagcc gccgatgtaa tagtgatgac aataatcaga tgtcttgcca 300

tactgtacag ttatcatcaa ttcagaaatc tcagaaaact aggatccaaa tatatattag 360

gttttgcagg actatttgta gtctgctcca gtttcgtgtt tatatctgca gtattaaaca 420

tcctccaaat cgaagtagcg gacctaaaag atgctctgtt ctttttcttg ttgctaatag 480

atctctcaaa agctgttaaa ttggctcaat acgctctaac tgcctcaaac caagaagaaa 540

taagtaaaaa catagcaatg ggagttgctg ttttagggcc taccataacg ctagatacta 600

tcgtagaaac cttagtaata ggagtgggaa ctttgtcagg agttcagcgt ctagaaatgc 660

tatcgtattt tgcgtgtctg tcagtcattg tgaattatat aattttgtta actttttacc 720

cagcttgttt agctttaatg ttagatttaa gcaaagtaag tgattattac aaacaagaac 780

aagttaccag aatactagtg gacgaaaaag ataagtccaa tccagctgca caacgtgtta 840

agatgattat gtctcttgga ctcacatagt acatattcat agt 883

<210>288

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>288

gttatacggg gaactatgtg ccactcac 28

<210>289

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>289

gactgacaga cacgcaaaat acgatagc 28

<210>290

<211>586

<212>DNA

<213>Diabrotica virgifera

<400>290

gttatacggg gaactatgtg ccactcaccc atgggaagtt atttttactg tcatcacctt 60

cactgtatgt tttttgactt tggataaacc gcaagggaca aaggcctttc cacggactac 120

ctcaacatgt agcacctgca ccgaagacca ttttatagcc gccgatgtaa tagtgatgac 180

aataatcaga tgtcttgcca tactgtacag ttatcatcaa ttcagaaatc tcagaaaact 240

aggatccaaa tatatattag gttttgcagg actatttgta gtctgctcca gtttcgtgtt 300

tatatctgca gtattaaaca tcctccaaat cgaagtagcg gacctaaaag atgctctgtt 360

ctttttcttg ttgctaatag atctctcaaa agctgttaaa ttggctcaat acgctctaac 420

tgcctcaaac caagaagaaa taagtaaaaa catagcaatg ggagttgctg ttttagggcc 480

taccataacg ctagatacta tcgtagaaac cttagtaata ggagtgggaa ctttgtcagg 540

agttcagcgt ctagaaatgc tatcgtattt tgcgtgtctg tcagtc 586

<210>291

<211>816

<212>DNA

<213>Diabrotica virgifera

<400>291

gatagtagtc aatcagccag ccatgtggta gaaatcaaaa tttatgctcg atctttatac 60

cacctccgcg ttttcttcca aaaacaccgg atgctgataa ggaccagtat tgtttcatta 120

atttaaaaca gatggatggg tgcactgtca agttcagaga attcaattcg attatggttt 180

agcgatacgg tgacaataaa tttgaattat tgtcacattt atcaagaaag tgtacttaat 240

ttataaacag tctgtgataa tatagagctg aatatctaga ccagttatta acatgacgat 300

taagaatatt tgttgccttg gcgctggtta cgtaggtggg ccaacctgca gtgtgatagc 360

tttaaaatgt aaagatatta aagtaacagt tgctgatctt agcgaagaac gaatagctca 420

atggaactcc gacagactcc caatttatga gcctggatta gacaaagtag tacaagagtg 480

cagaggtagg aatttattct tcaccactaa ccttactcaa gcaatattag atgctgactt 540

gatatttata tcagtgaata caccaacaaa actgaacgga aatggcaagg gaagggcacc 600

tgatctaaaa tacatagagg gagcagcaag aatcatagct gatactgctc aaactaacaa 660

aatcgttgta gaaaaaagta ctgttccagt acgagcagct gaaagtattt cagatatctt 720

aacagctaat aaaaagccaa aagtgacaca tcagattttg tctaatccag aatttctagc 780

ggaaggtact gcaatcaatg atctgctcat cctgat 816

<210>292

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>292

gatagtagtc aatcagccag ccatgtgg 28

<210>293

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>293

gcagtacctt ccgctagaaa ttctgga 27

<210>294

<211>792

<212>DNA

<213>Diabrotica virgifera

<400>294

gatagtagtc aatcagccag ccatgtggca gaaatcaaaa tttatgctcg atctttatac 60

cacctccgcg ttttcttcca aaaacaccgg atgctgataa ggaccagtat tgtttcatta 120

atttaaaaca gatggatggg tgcactgtca agttcagaga attcaattcg attatggttt 180

agcgatacgg tgacaataaa tttgaattat tgtcacattt atcaagaaag tgtacttaat 240

ttataaacag tctgtgataa tatagagctg aatatctaga ccagttatta acatgacgat 300

taagaatatt tgttgccttg gcgctggtta cgtaggtggg ccaacctgca gtgtgatagc 360

tttaaaatgt aaagatatta aagtaacagt tgctgatctt agcgaagaac gaatagctca 420

atggaactcc gacagactcc caatttatga gcctggatta gacaaagtag tacaagagtg 480

cagaggtagg aatttattct tcaccactaa ccttactcaa gcaatattag atgctgactt 540

gatatttata tcagtgaata caccaacaaa actgaacgga aatggcaagg gaagggcacc 600

tgatctaaaa tacatagagg gagcagcaag aatcatagct gatactgctc aaactaacaa 660

aatcgttgta gaaaaaagta ctgttccagt acgagcagct gaaagtattt cagatatctt 720

aacagctaat aaaaagccaa aagtgacaca tcagattttg tctaatccag aatttctagc 780

ggaaggtact gc 792

<210>295

<211>1215

<212>DNA

<213>Diabrotica virgifera

<400>295

cggacgcgtg gggttgaaag ttaggttaac tccttcacta aacttgctct tgttgtagat 60

acacgtgttt acagcaaata agttattttt taagtatatt gactgcaaat tttcacaatg 120

gctaacgatt tagaacaaaa gatcatcaaa caaatcgaat attacttcgg agacatcaat 180

ctgccgcgtg ataaattctt acaagagaaa atcaaagaag atgaagggtg gatcactctg 240

gatgtcttat taacgttcaa acgtctttcg tcgttatcta ctgaccaaga attaatcgca 300

aaagcagttg aaaagtctga aaatggccta gtgatggtaa gcgaagaccg taagaaactt 360

agacgcaatc cagagaaacc gagtccagaa ttgaatgagg aaagacgcaa agagatcatg 420

gggaggacag cctacgcaaa aggatttcca ttggatgaag aatttaacaa cattatcact 480

ttcctagatg catatggacc agtcgaatca tgcagtcgta gatccaccaa agaccataaa 540

tttaagggtt cctgttttat tattttcaaa gatgtcgaaa cctgcaagaa attcgtagaa 600

gcggaaagta tcaaatatgg agaaacagaa ctaatcagga aattccaaca agtatatttt 660

gacgacaaaa aaaaagagat acaggacaga aaaaacgaga aagctgctag caaggtagct 720

gcaaaatcgg gcacaccgct tcagtttccc aaaggagcca ttattcattt ctctggaatc 780

gaggaaggtg taaaattatt gagggaagat cttaaagaaa aggtcaagga agtcggcaaa 840

attgaagtaa aattcgttga ttttaatcaa ggtgatttgg agggatacct acgtttcgca 900

gaagaaaacg aagctgtcga gttctttaag acagttgata atgacacact tgaaattaaa 960

gatcaaaaag tcaaattgag agtgttagaa ggagacgaag aggaggaata tctgaagaaa 1020

acttcagatt ctattctaaa catgagaaaa cataagaaac aaaattctaa taacagaaaa 1080

cgaaagggca actttggtgg cggaagaaga gatgctaaag ttagtaaaag ggactaataa 1140

acaaaatatg ttatcatctt tatctaattg tttagatatt gtaaaaatta ggttaaaaat 1200

aaaggttttt tttac 1215

<210>296

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>296

caagagaaaa tcaaagaaga tgaagggtgg 30

<210>297

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>297

ccaaagttgc cctttcgttt tctgttatta gaat 34

<210>298

<211>895

<212>DNA

<213>Diabrotica virgifera

<400>298

caagagaaaa tcaaagaaga tgaagggtgg atcactctgg atgtcttatt aacgttcaaa 60

cgtctttcgt cgttatctac tgaccaagaa ttaatcgcaa aagcagttga aaagtctgaa 120

aatggcctag tgatggtaag cgaagaccgt aagaaactta gacgcaatcc agagaaaccg 180

agtccagaat tgaatgagga aagacgcaaa gagatcatgg ggaggacagc ctacgcaaaa 240

ggatttccat tggatgaaga atttaacaac attatcactt tcctagatgc atatggacca 300

gtcgaatcat gcagtcgtag atccaccaaa gaccataaat ttaagggttc ctgttttatt 360

attttcaaag atgtcgaaac ctgcaagaaa ttcgtagaag cggaaagtat caaatatgga 420

gaaacagaac taatcaggaa attccaacaa gtatattttg acgacaaaaa aaaagagata 480

caggacagaa aaaacgagaa agctgctagc aaggtagctg caaaatcggg cacaccgctt 540

cagtttccca aaggagccat tattcatttc tctggaatcg aggaaggtgt aaaattattg 600

agggaagatc ttaaagaaaa ggtcaaggaa gtcggcaaaa ttgaagtaaa attcgttgat 660

tttaatcaag gtgatttgga gggataccta cgtttcgcag aagaaaacga agctgtcgag 720

ttctttaaga cagttgataa tgacacactt gaaattaaag atcaaaaagt caaattgaga 780

gtgttagaag gagacgaaga ggaggaatat ctgaaaaaaa cttcagattc tattctaaac 840

atgagaaaac ataagaaaca aaattctaat aacagaaaac gaaagggcaa cttgg 895

<210>299

<211>822

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(807)

＜223〉n=a, c, g or t/u

<400>299

caattagctc aaagagccag agacgagaga gctgggatta aggcagctcc tagtcattcc 60

aagggaggcg tggacgagga agaacacgaa agagacttgc ttcgtcaaga tcgtcataaa 120

gagcgagctc gagatcgtaa tttagcgcgt gcagctcccg ataagcgcag taaacttcag 180

cgagagcgcg aaagagacat ctcagaacaa attgctctcg gtatgccagc gaaaggtgca 240

ccatccaacg acactcagtt tgatcaaaga ttatttggtc aggctcaagg tcttggtcac 300

ggctatgggg atgatgaagc ttataatgtc tatgataaac cttggagaga tggaggatcc 360

atggcaaatc acatttatcg accaagtaaa aatattgata aagatatgta tggtggagaa 420

gacattgaaa aagctattag aactaatagg tttgtaccag ataaagaatt tgggggtact 480

gatcgttctg gagcaggccg atcaggtcct gtgcaattcg aaaaagaaga agatccgttc 540

ggattggatc agttcttgtc gcaagctaag agagccagta aaagaaaaga acctgaaaag 600

agagacgaca gggataagag gaaaagaaga gagtaaaagt attttacagt ttctttttga 660

gacctttata aaataaatat aggtatactg gtatacataa ttctgatgta tttttaaaaa 720

agttatattt gatattatgt aactaattgt aataggtaaa ctcaacaata catttttcag 780

ataatttttg tgaattattg taattanata tctataacac ac 822

<210>300

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>300

caagggaggc gtggacgagg aag 23

<210>301

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>301

ctctcttctt ttcctcttat ccctgtcgtc tc 32

<210>302

<211>573

<212>DNA

<213>Diabrotica virgifera

<400>302

cagggaggcg tggacgagga agaacacgaa agagacttgc ttcgtcaaga tcgtcataaa 60

gagcgagctc gagatcgtaa tttagcgcgt gcagctcccg ataagcgcag taaacttcag 120

cgagagcgcg aaagagacat ctcagaacaa attgctctcg gtatgccagc gaaaggtgca 180

ccatccaacg acactcagtt tgatcaaaga ttatttggtc aggctcaggg tcttggtcac 240

ggctatgggg atgatgaagc ttataatgtc tatgataaac cttggagaga tggagggtcc 300

atggcaaatc acatttatcg accaagtaaa aatattgata aagatatgta tggtggagaa 360

aacattgaaa aagctattag aactaatagg tttgtaccag ataaagaatt tgggggtact 420

gatcgttctg gagcaggccg atcaggtcct gtgcaattcg aaaaagaaga agatccgttc 480

ggattggatc agttcttgtc gcaagctaag agagccagta aaagaaaaga acctgaaaag 540

agagacgaca gggataagag gaaaagaaga gag 573

<210>303

<211>755

<212>DNA

<213>Diabrotica virgifera

<400>303

aatgataact ttgggtgggg atgctgattt tgaaatggtt gatacgtatg atccattttc 60

agagggaatt cagtttaatg tgaggcctcc gaaaaaatct tggaagaaaa tttctaactt 120

atctggagga gaaaaaacgt tatcatcttt ggcccttgtc tttgctctgc attattacaa 180

accttcgcca ctttatgtaa tggacgaaat tgacgcagcc ttagatttca aaaatgtgtc 240

aattgttgga aattatatta agcaacgtac caaaaacgct caattcatta taatctccct 300

tcgatccaac atgttcgaac tgtgcgataa tctcatagga atttacaaaa cgtacgattg 360

cacgaagtct ataacgatag atccaagagt atatgacgag caaacaaaac ataacgaaaa 420

gcgagtgtcg cttggatcga ccaacgaaga gagtagacga acgtctgtgc ccgcttctgg 480

tactggagat tctggagata aaaatccaga aagaaatgtt agcaatgaag ttgtagacga 540

acaacgttcg aacttgatgg aggttgataa tgtcatccag agtacaaata atgccaggga 600

ttcatctggt ctgtctagta gcatgagtga aaatgaaaat agtttacaag aatcccaagt 660

gcctgaaata tgaaggggtc agtttcgtga tttagttact acttcttgat ataaatgtag 720

catttttagt tactttaata catttacata tttgt 755

<210>304

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>304

ggatgctgat tttgaaatgg ttgatacg 28

<210>305

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>305

cttcatattt caggcacttg ggattcttg 29

<210>306

<211>656

<212>DNA

<213>Diabrotica virgifera

<400>306

ggatgctgat tttgaaatgg ttgatacgta tgatccattt tcagagggaa ttcagtttaa 60

tgtgaggcct ccgaaaaaat cttggaagaa aatttctaac ttatctggag gagaaaaaac 120

gttatcatct ttggcccttg tctttgctct gcattattac aaaccttcgc cactttatgt 180

aatggacgaa attgacgcag ccttagattt caaaaatgtg tcaattgttg gaaattatat 240

taagcaacgt accaaaaacg ctcaattcat tataatctcc cttcgatcca acatgttcga 300

actgtgcgat aatctcatag gaatttacaa aacgtacgat tgcacgaagt ctataacgat 360

agatccaaga gtatatgacg agcaaacaaa acataacgaa aagcgagtgt cgcttggatc 420

gaccaacgaa gagagtagac gaacgtctgt gcccgcttct ggtactggag attctggaga 480

taaaaatcca gaaagaaatg ttagcaatga agttgtagac gaacaacgtt cgaacttgat 540

ggaggttgat aatgtcatcc agagtacaaa taatgccagg gattcatctg gtctgtctag 600

tagcatgagt gaaaatgaaa atagtttaca agaatcccaa gtgcctgaaa tagaag 656

<210>307

<211>428

<212>DNA

<213>Diabrotica virgifera

<400>307

cggacgcgtg ggctgaaatc caaatttttt ataaatataa ccttaaagaa gtctgtaaag 60

atttaagcat gatttaacga aatattaaat ttttaacttt aaaatgccga actcagatat 120

tgatgataac gaaaaagtaa ctgggcccgt cgacaatgca tgggaactga aaattccaga 180

attcaaacca gaagataatc cacatggatt attagaggaa agtagtttcg ccacattatt 240

tccacaatac agagaacaat atttaagaca agtatggcct ttagttaaga aaactctggg 300

tgaatataat ataaaagcag aactagatgt tgtagaaggt agtatgactg ttaaaaccac 360

aaggcaaaca tgggatcctt acattattat taaagcaaga gatgtgataa agttaatgtc 420

tagaagtg 428

<210>308

<211>36

<212>DNA

<213>Diabrotica virgifera

<400>308

cgaactcaga tattgatgat aacgaaaaag taactg 36

<210>309

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>309

catgtttgcc ttgtggtttt aacagtcata ctac 34

<210>310

<211>265

<212>DNA

<213>Diabrotica virgifera

<400>310

cgaactcaga tattgatgat aacgaaaaag taactgggcc cgtcgacaat gcatgggaac 60

tgaaaattcc agaattcaaa ccagaagata atccacatgg attattagag gaaagtagtt 120

tcgccacatt atttccacaa tacagagaac aatatttaag acaagtatgg cctttagtta 180

agaaaactct gggtgaatat aatataaaag cagaactaga tgttgtagaa ggtagtatga 240

ctgttaaaac cacaaggcaa acatg 265

<210>311

<211>657

<212>DNA

<213>Diabrotica virgifera

<400>311

acgcgtccgc ccacgcgtcc gcgactgccg gccattattt gtaagcggcg aaagattcgg 60

cgttgctttg taaaattgtt gtgaatctac cggcatcaaa agcctaaaaa gcgtgtagct 120

tttacgtagt tatttaactg tagctacttt acaaatttca tcaaaatggg cgatgaaaag 180

aaggccagcg atagcgaaca cattaatctc aaagtacttg gtcaggataa tgcagtggta 240

caattcaaaa tcaaaaaaca caccccatta aaaaaattga tgaccgccta ttgtgaacgt 300

gctggtataa gcacacaagt agtcagattt agatttgatg gcaaccccat aaacgacaca 360

gatactccta attctttaga gatggatgaa ggagacacaa ttgaagtata tcaacaacag 420

acaggtggtt tcttctatcg gtttctattc atttaaataa tcacactgta ttgttcttgt 480

attttcttat ttgctaaatt aaactagttt taaataagta agttaatttt aatataattt 540

ttaattcatg tcaactgaag ttacaatagt tctgtaagaa ttcgactatt tgtgtttttg 600

gactcttatt ttgtaattaa aacacttaga atacaaacat ttttcttata aaaaaaa 657

<210>312

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>312

gaaagattcg gcgttgcttt gtaaaattg 29

<210>313

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>313

gaatagaaac cgatagaaga aaccacctgt c 31

<210>314

<211>401

<212>DNA

<213>Diabrotica virgifera

<400>314

gaaagattcg gcgttgcttt gtaaaattgt tgtgaatcta ccggcatcaa aagcctaaaa 60

agcgtgtagc ttttacgtag ttatttaact gtagctactt tacaaatttc atcaaaatgg 120

gcgatgaaaa gaaggccagc gatagcgaac acattaatct caaagtactt ggtcaggata 180

atgcagtggt acaatttaaa atcaaaaaac acaccccatt aaaaaaattg atgaccgcct 240

attgtgaacg tgctggtata agcacacaag tagtcagatt tagatttgat ggcaacccca 300

taaacgacac agatactcct aattctttag agatggatga aggagacaca attgaagtat 360

atcaacaaca gacaggtggt ttcttctatc ggtttctatt c 401

<210>315

<211>822

<212>DNA

<213>Diabrotica virgifera

<400>315

aaacgacgat tcagtgcacc gacgcaacgt acgccagcca attgtcccta gagaatccca 60

aaggattttc tagagatgct tttggaaggc agagtatgtc agagaagagg catgcgacgt 120

tggatgccaa gaataccgac acatatcaga aatcgaagaa gattagggag gagaggaaga 180

tgaaagaaat aaatgctgat aaattaggcc aattaggacc aagtttaggc atgaagaaat 240

cttcaagctt ggaatcgtta cagacaatgg tacaagagat tcaaatgcaa gaagaaggcg 300

acccagctta tagttataga ggacccaatg gagctttaaa agtaatcagg gggaaaggct 360

gtgaagaaag ctttagggca gctgtagtgg atccaaatca ccgaagtgag atcggagcca 420

aaaagcattg gttgcttgac ggtccaatgg aaaacgaacg agaagtggat ggcttcagca 480

atgctcgagg aggtcctagg caatcttctc tgaatgcagc tattgatata aagaataaat 540

cagcaaagaa gaaacctggt atatttaaag gcattggttc gatgttcagg tttggcaaac 600

atcgtaaact ggacttccaa acagcagagg ctgcccaaca ctatcatcat acaaacgaat 660

tcgattcaaa tgccaacgta cctcaacaac acaccgcaga taccgaaaac cgaacgcaaa 720

gtagtcaaac agaacggacg caagagcatt cacaaaaagt acctctaaat agcaaccccc 780

agaaccaaaa cacttcccaa ggatctcacc accagacgca ag 822

<210>316

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>316

gatgccaaga ataccgacac atatcagaaa tc 32

<210>317

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>317

cttgcgtctg gtggtgagat ccttg 25

<210>318

<211>700

<212>DNA

<213>Diabrotica virgifera

<400>318

gatgccaaga ataccgacac atatcagaaa tcgaagaaga ttagggagga gaggaagatg 60

aaagaaataa atgctgataa attaggccaa ttaggaccaa gtttaggcat gaagaaatct 120

tcaagcttgg aatcgttaca gacaatggta caagagattc aaatgcaaga agaaggcgac 180

ccagcttata gttatagagg acccaatgga gctttaaaag taatcagggg gaaaggctgt 240

gaagaaagct ttagggcagc tgtagtggat ccaaatcacc gaagtgagat cggagccaaa 300

aagcattggt tgcttgacgg tccaatggaa aacgaacgag aagtggatgg cttcagcaat 360

gctcgaggag gtcctaggca atcttctctg aatgcagcta ttgatataaa gaataaatca 420

gcaaagaaga aacctggtat atttaaaggc attggttcga tgttcaggtt tggcaaacat 480

cgtaaactgg acttccaaac agcagaggct gcccaacact atcatcatac aaacgaattc 540

gattcaaatg ccaacgtacc tcaacaacac accgcagata ccgaaaaccg aacgcaaagt 600

agtcaaccag aacggacgca agagcattca caaaaagtac ctcaaaatag caacccccag 660

aaccaaaaca cttcccaagg atctcaccac cagacgcaag 700

<210>319

<211>1321

<212>DNA

<213>Diabrotica virgifera

<400>319

ttagccaagc ggcatggaag taaagaccca aaggagctct gttttcattg taatgcctat 60

cgaaattata atttaagaag ttataaaatt gatctccagt aagaatgttg gaacatgagt 120

caaccatggc acaacctggt aaatactcat cattttcagg atcttctctt ctatctagtt 180

cgttaagtac catttcccat actgcatgtg atcttgttgg gcaattttgg aggtttccgt 240

gacatctgtg tggcattcta aaatacatag tatagggcca tagtggtggg ttatttaagg 300

gtgcggtgat agtggagtcg tataagaagg cttcttcttc catcatagta aattggttgt 360

tacctcctac tcttagataa ggggctctta ctcctactac gctgttatcg gtaatattgg 420

cgaatttctc agtgatgact cgcatacctc ccatttcttt aacccagtca tttacactgg 480

cgttggacca gaagttttcg tcatcattgt gcgatattga atgtactgcg atttcgtgac 540

cttttctatg catttcttgg actgccgagt agttggtgta cttgtgagac acgaagaatg 600

tagccttgat atgacaaccg ttggggtttt tccgttcacc gttgaaaatg ttcttgtata 660

gaccgatgtt gttattattt atggcgtcgt caaatgtgac agttatcatt tgaggtacat 720

ctttggctgg taaatcattg ggaatgcctg taccgtcctc tgagcagaaa caatcaggga 780

gaacgcatac tgcggggtcg cagggtgggg cactattggg gtcactatcg atatcgcagg 840

tagtttcatc agacccatcc tggcaatcct tttccccgtt acaaaagagc cctctttcta 900

tacactgacc atctccgcag gccaattcac cttcgttaca tacgggtttg tccgtaaaca 960

acatgggttt tacccgccgc tctttgtttt tcaatttgca gtttttaact gagtccttcc 1020

agtcgcatgt ttgcttttcg atgtcgaaat atagaccggc tggacaacga atggcttgca 1080

ggcccgaaga tgtacattga atgacgtctc gacaggtgtc cccctcccct cccactaaac 1140

gaaaccactc gcccgcgtct ttgtctttgc atatttcggc ctcgaaactc tcctctttct 1200

tcggttcatc gtctttggcc tgctgttgtg ccaaaccgct tgaggctacc aacgcaacca 1260

agaaaatcgt cgttgcgaag tacgccatga cacttaaaca actgaaagta ccggacgcac 1320

a 1321

<210>320

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>320

cagttgttta agtgtcatgg cgtacttcg 29

<210>321

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>321

ggaggtttcc gtgacatctg tgtgg 25

<210>322

<211>1075

<212>DNA

<213>Diabrotica virgifera

<400>322

cagttgttta agtgtcatgg cgtacttcgc aacgacgatt ttcttggttg cgttggtagc 60

ctcaagcggt ttggcacaac agcaggccaa agacgatgaa ccgaagaaag aggagagttt 120

cgaggccgaa atatgcaaag acaaagacgc gggcgagtgg tttcgtttag tgggagggga 180

gggggacacc tgtcgagacg tcattcaatg tacatcttcg ggcctgcaag ccattcgttg 240

tccagccggt ctatatttcg acatcgaaaa gcaaacatgc gactggaagg actcagttaa 300

aaactgcaaa ttgaaaaaca aagagcggcg ggtaaaaccc atgttgttta cggacaaacc 360

cgtatgtaac gaaggtgaat tggcctgcgg agatggtcag tgtatagaaa gagggctctt 420

ttgtaacggg gaaaaggatt gccaggatgg gtctgatgaa actacctgcg atatcgatag 480

tgaccccaat agtgccccac cctgcgaccc cgcagtatgc gttctccctg attgtttctg 540

ctcagaggac ggtacaggca ttcccaatga tttaccagcc aaagatgtac ctcaaatgat 600

aactgtcaca tttgacgacg ccataaataa taacaacatc ggtctataca agaacatttt 660

caacggtgaa cggaaaaacc ccaacggttg tcatatcaag gctacattct tcgtgtctca 720

caagtacacc aactactctg cagtccaaga aatgcataga aaaggtcacg aaatcgcagt 780

acattcaata tcgcacaatg atgacgaaaa cttctggtcc aacgccagtg taaatgactg 840

ggttaaagaa atgggaggta tgcgagtcat cactgagaaa ttcgcaaata ttaccgataa 900

cagcgtagta ggagtaagag ccccttatct aagagtagga ggtaacaacc aatttactat 960

gatggaagaa gaagccttct tatacgactc cactatcacc gcaccattaa ataacccacc 1020

actatggccc tatactatgt atttcagaat gccacacaga tgtcaggaaa cctcc 1075

<210>323

<211>1703

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(1641)

＜223〉n=a, c, g or t/u

<400>323

tgaaagtcaa cattggtgaa gagtagtact gtccaacgga gcatgcgcag tgcgaaaaaa 60

gcacttgtat aaataaaacg aaagtctgcg atcacttctt ggctagtgct tgaaatttca 120

gcatggctga caaaagacta tggctgtgtt gttttttatt attgacaatt gcttacgctc 180

aagatgaaga tgatgacgat tcaggggaac cacagatcga ccagctgtgt gaaaacaggc 240

ctgctgatga atattttagg ttaagcactg atggagactg cagagaagtt gtaaggtgtg 300

atctcaattc agatcttaag ggcggtactc gattggcagc ggtaaaatgt ccgacaggac 360

ttgcttttga tcttttccga caaacttgcg attggaaagt caatgtcaaa aattgtgata 420

tcgtagaaaa accaagaaaa gtccttccaa ttttgaaaac cgacgaacca agatgtccag 480

aaggaaagct gatgtgtggt aatgaagaat gtatcgacaa agatcttttc tgtaatggaa 540

agccagactg taaagatgaa tcagacgaga atgcatgcga tgttgatagt gatcccaacc 600

gtgcacctga ctgtgaccca actcaatgtg taattccgga ctgtttctgc tcagcggacg 660

gaactagaat tcctggcgcg ctagaaccag caaatgtacc acaaatgata accatttcgt 720

tcaatggtgc cgttaatgta gataacatcg atctatatga agaaatcttt tcaggcacta 780

gacttaatcc aaatggttgc caaattaaag gaaatttctt cgtttctcac aaatacacaa 840

actactcagc agtacaggac ctacacagac gtggtcatga aatcgcggtg ttttcattga 900

ctcataaaga ggatccaaac tactggtccc agggttctta tgacgactgg ttggctgaaa 960

tggctggagc tagattgatt attgaaagat ttgccaacat taccgatgga tcaataattg 1020

gagtgagagc tccatacctc agagtaggag gaaacagaca atttgaaatg atgacagatc 1080

aattctttat ttatgattca tcaattacag cctctcttgg acgtgtccca atctggcctt 1140

atactttata cttcaggatg ccacataagt gcaacggaaa tgcccacaac tgtcccagca 1200

gaagccatcc cgtttgggaa atagtcatga acgaattgga cagacgtgat gatcctactt 1260

ttgacgaatc actacctggt tgccacatgg tagactcctg ttctaatgtt caaactggag 1320

atcaatttgc aagactactc agacacaatt tcaacagaca tttgaattca aaccgagctc 1380

cgttgggtct taacttccat gcctcatggt taaagagtaa gaaagaattc aaagaagaac 1440

ttattaaatt cattgaagat atgttggccc gaaatgatgt ttatttcgta acaaacagtc 1500

aagtcatcca atgggttcag aatcccacag aactcagtgg attaagagac ttccaagaat 1560

ggaaagaaaa gtgtgacatt aaaggtcaac cttactgttc attgcccaat tcatgtccat 1620

tacataccag agaactgcca nggtgaactc tacgattgtt tacctgtatg gaatgtccca 1680

acaattatcc atggattttg gat 1703

<210>324

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>324

gacgaaccaa gatgtccaga aggaaagc 28

<210>325

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>325

gttgggacat tccatacagg taaacaatcg 30

<210>326

<211>1221

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(271)..(358)

＜223〉n=a, c, g or t/u

<400>326

gacgaaccaa gatgtccaga aggaaagctg atgtgtggta atgaagaatg tatcgacaaa 60

gatcttttct gtaatggaaa gccagactgt aaagatgaat cagacgagaa tgcatgcgat 120

gttgatagtg atcccaaccg tgcacctgac tgtgacccaa ctcaatgtgt aattccggac 180

tgtttctgct cagcggacgg aactagaatt cctggcgcgc tagaaccagc aaatgtacca 240

caaatgataa ccatttcgtt caatggtgcc ngttaatgta gataacatcg atctatatga 300

agaaatcttt tcaggcacta gacttaatcc aaatggttgc caaattaaag gaaatttnct 360

tcgtttctca caaatacaca aactactcag cagtacagga cctacacaga cgtggtcatg 420

aaatcgcggt gttttcattg actcataaag agggtccaaa ctactggtcc cagggttctt 480

atgacgactg gttggctgaa atggctggag ctagattgat tattgaaaga tttgccaaca 540

ttaccgatgg atcaataatt ggagtgagag ctccatacct cagagtagga ggaaacagac 600

aatttgaaat gatgacagat caattcttta tttatgattc atcaattaca gcctctcttg 660

gacgtgtccc aatctggcct tatactttat acttcaggat gccacataag tgcaacggaa 720

atgcccacaa ctgtcccagc agaagccgtc ccgtttggga aatagtcatg aacgaattgg 780

acagacgtga tgatcctact tttgacgaat cactacctgg ttgccacatg gtagactcct 840

gttctaatgt tcaaactgga gatcaatttg caagactact cagacacaat ttcaacagac 900

atttgaattc aaaccgagct ccgttgggtc tcaacttcca tgcctcatgg ttaaagagta 960

agaaagaatt caaagaagaa cttattaaat tcattgaaga tatgttggcc cgaaatgatg 1020

tttatttcgt aacaaacagt caagtcatcc aatgggttca gaatcccaca gaactcagtg 1080

gattaagaga cttccaagaa tggaaagaaa agtgtgacat taaaggtcaa ccttactgtt 1140

cattgcccaa ttcatgtcca ttacatacca gagaactgcc aggtgaaact ctacgattgt 1200

ttacctgtat ggaatgtccc a 1221

<210>327

<211>826

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(658)..(684)

＜223〉n=a, c, g or t/u

<400>327

caaattgttt attacaagtt tattaccatg agagtgtata taatattatt tagcgtcttt 60

tgcttgagcc attgcgctcc acaggaaaac aaaaataaat ttccagaaca ataccaaggt 120

caacacatac caattgtaag gcaagaacaa gaagtgaatt atgatggcac ctacgaatac 180

aactttgaaa ctggaaatgg catagtccaa gaagaaaaag gatttttgaa aaacgccggt 240

acaaaggaag aagctcaagt cgctcaaggt ttttcctcat atacttcccc cgaaggagta 300

aaaatagaac tccggtacat cgcagacgaa aacggtttcc agccaatcgg agaccaccta 360

ccgactccgc caccaatccc tgaggctatt ttacgagcgc taagtgtact gaaacagttg 420

ggtaatttga atgaagacca agaagaaaat aacaacatta gatgaagaga gagtgtgatc 480

aaatcgttat tttggataag tcccttcata tttcaaaatg gtaccatact aactatgaaa 540

tacttcaaag aattaagatc tttttaaaat acgtcaacac tttagagtag gaaacagcgg 600

tggaacctcg caaaatgtac acaagttcgg ttttattttt ttgcaggaaa atcaaggngt 660

gcttataatg aaactaacat tttnctaaaa aatttcgccc ctgaaccccc ctttttatcc 720

ctttgaaggg gggtatttgt ggtttttgcg aaacgaagcc cttcctgtat acgttttaca 780

aaagaatgta ctaaatagta aaatgaagag gactataatt tgctac 826

<210>328

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>328

ccagaacaat accaaggtca acacatacc 29

<210>329

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>329

gaggttccac cgctgtttcc tactc 25

<210>330

<211>505

<212>DNA

<213>Diabrotica virgifera

<400>330

agaacaatac caaggtcaac acataccaat tgtaaggcaa gaacaagaag tgaattatga 60

tggcacctac gaatacaact ttgaaactgg aaatggcata gtccaagaag aaaaaggatt 120

tttgaaaaac gccggtacaa aggaagaagc tcaagtcgct caaggttttt cctcatatac 180

ttcccccgaa ggagtaaaaa tagaactccg gtacatcgca gacgaaaacg gtttccagcc 240

aatcggagac cacctaccga ctccgccacc aatccctgag gctattttac gagcgctaag 300

tgtactgaaa cagttgggta atttgaatga agaccaagaa gaaaataaca acattagatg 360

aagagagagt gtgatcaaat cgttattttg gataagtccc ttcatatttc aaaatggtac 420

catactaact atgaaatact tcaaagaact aagatctttt taaaatacgt caacacttta 480

gagtaggaaa cagcggtgga acctc 505

<210>331

<211>731

<212>DNA

<213>Diabrotica virgifera

<400>331

ttggtaaagt ttgttgtagt ttcagtgatc ttcattttga actctttttt tttttcaata 60

tgcgaactac gtactttaca ttagttgtaa tattagctgt agctgtacaa gaacatgcat 120

ctcaacaatt accagtaacg caagcttgct tgggatgtat atgtgaagct atttccaact 180

gtaatgtaac tagcggttgt tctggtgagg tttgtggacc atttcggatc acctgggcat 240

attgggcaga tgctggaaag cccaccgtga aaggagtatc accagaagac aataatgcat 300

atggatcttg tgctactgat acgtattgtt ctgcattagc tgttcaagga tatatgaata 360

aatttcaaca ggattgcaat ggtgatggta aaatagactg tgacgattat gcaagtattc 420

acacgcatgg tggatatggc tgcaaaggaa ctaatctacc acaacctttt ggaaatcgat 480

attatcaatg taaagagata gttggacgta gaccagagta atgttggagt aaccccagga 540

ttgagaacag gaagattgat agaaacgcat ttactactct atgaattttg ttggatgatt 600

attgcaactt aatgtaattc aatatgttta agggctgtaa ttacaattaa ataaaaataa 660

ctgacatcat agaatacact attgtaacaa ctattttttt aggtatagta ttttaagtaa 720

actctttttt t 731

<210>332

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>332

gcatctcaac aattaccagt aacgcaagc 29

<210>333

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>333

gtttctatca atcttcctgt tctcaatcct gg 32

<210>334

<211>450

<212>DNA

<213>Diabrotica virgifera

<400>334

gcatctcaac aattaccagt aacgcaagct tgcttgggat gtatatgtga agctatttcc 60

aactgtaatg taactagcgg ttgttctggt gaggtttgtg gaccatttcg gatcacctgg 120

gcatattggg cagatgctgg aaagcccacc gtgaaaggag tatcaccaga agacaataat 180

gcatatggat cttgtgctac tgatacgtat tgttctgcat tagctgttca aggatatatg 240

aataaatttc aacaggattg caatggtgat ggtaaaatag actgtgacga ttatgcaagt 300

attcacacgc atggtggata tggctgcaaa ggaactaatc taccacaacc ttttggaaat 360

cgatattatc aatgtaaaga gatagttgga cgtagaccag agtaatgttg gagtaacccc 420

aggattgaga acaggaagat tgatagaaac 450

<210>335

<211>878

<212>DNA

<213>Diabrotica virgifera

<400>335

gtgattgtgg tgttttatcg gtgttaagac aggatgggtt tcggtcaaaa aattctagtc 60

cctctagtcc ttatagaaat gtccctactg gttacatcca gaataatata tgaccatcag 120

caagaagata atcacgaagg gcaacaagtt caatacacca attactatgg tcacgaagag 180

tctgccacaa cagcacattt ccaacaaccc gtccagtttc ccgaggaaca tagccaaggt 240

ttgcacaaag aggaacacat cgattattac gcaccagcca agtattcctt caaatatgga 300

gtcaacgact accacactgg cgatataaaa agccaacacg aatccagaga cggagatact 360

gtcaaaggcc aatacacact tgtagaaccc gacggatcta tcagaactgt agactacact 420

gctgacaaac acagtggatt taacgcagta gtgcgcaaat caggtcccac aaaacacata 480

gaaggaaggc atctggaaca ccaacccgaa gaagagcaac atatacctca gcatcatttg 540

gcagaagatc atgttttacc gcagtatcaa ctggaacacc aacaggcttt ggaagaagat 600

gttgaagaag gacatctgga agctgaagat gctagtcaat atgaagaaga agagcattag 660

gtgttatatt aagtttaagg acgtaatggt ttgtaaataa gcgaactcaa agattaaagt 720

agtatctttt aaaattattg gagaaaataa gaatttttgt ttgtaagaaa aagtatcatc 780

atcaactata tttttttata acacttttaa cattttggga acgtaatatt cgaatttata 840

cactcacaga caaaaatctt gcatattttg aaattatg 878

<210>336

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>336

ggtgttaaga caggatgggt ttcggtc 27

<210>337

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>337

ggtgttccag ttgatactgc ggtaaaac 28

<210>338

<211>560

<212>DNA

<213>Diabrotica virgifera

<400>338

ggtgttaaga caggatgggt ttcggtcaaa aaattctagt ccctctagtc cttatagaaa 60

tgtccctact ggttacatcc agaataatat atgaccatca gcaagaagat aatcacgaag 120

ggcaacaagt tcaatacacc aattactatg gtcacgaaga gtctgccaca acagcacatt 180

tccaacaacc cgtccagttt cccgaggaac atagccaagg tttgcacaaa gaggaacaca 240

tcgattatta cgcaccagcc aagtattcct tcaaatatgg agtcaacgac taccacactg 300

gcgatataaa aagccaacac gaatccagag acggagatac tgtcaaaggc caatacacac 360

ttgtagaacc cgacggatct atcagaactg tagactacac tgctgacaaa cacagtggat 420

ttaacgcagt agtgcgcaaa tcaggtccca caaaacacat agaaggaagg catctggaac 480

accaacccga agaagagcaa catatacctc agcatcattt ggcagaagat catgttttac 540

cgcagtatca act gaacacc 560

<210>339

<211>831

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(807)

＜223〉n=a, c, g or t/u

<400>339

ctcagttttg tcgacggtgt tgatcgaaga cgagtcatga aatttttgat tgttgccgca 60

ttggtgtttg gagcctgttc ggcagaacca cagaggcggc ctcattccta ctatgacaac 120

caggctgaac aagacacaaa cgtcccacaa aactaccaag ataactcaga tcaataccaa 180

cagaattctg tccaacaaaa cccaccgcaa catcaggaaa aatcgcaata tgaaccacga 240

gtaaaagaaa ctatcaaatt cataccaatt atcagattcg ataaagaaca aggagaagcc 300

ggtaattaca aagctgcata tgaaaccgga aacaacatta tcgctgaaga agaaggttac 360

ttgaaggaac tcggtccaga tccagaccag caaggacaag ttatcaaatc gcaagtacaa 420

cagggtcact tcacatatac atcccctgaa ggagtagtta ttgaaactaa atatactgct 480

gacgaaaacg gtttccatgt tgaaggagat catttgccaa ccccaccacc agttagcgcc 540

gaagtacaaa agggattgga ccttattttt gctggtatca gagctcaaca ggaggcgtta 600

gaacaaagat taaaagatca cccagaagaa agagacagac ctgaaaatta cgacgatggt 660

caataccgac cggcacaaca ggagtaattt tactctttat ttatagttta gcaccatcat 720

tctgtgattt tgtcgtcatt tctatagaat atcttctgta cagactgaat aagattgata 780

atacactgtg gaaaaaaaat tgataanaat caataaaaat ctttttacac a 831

<210>340

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>340

gattgttgcc gcattggtgt ttgg 24

<210>341

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>341

gacgacaaaa tcacagaatg atggtgc 27

<210>342

<211>690

<212>DNA

<213>Diabrotica virgifera

<400>342

gattgttgcc gcattggtgt ttggagcctg ttcggcagaa ccacagaggc ggcctcattc 60

ctactatgac aaccaggctg aacaagacac aaacgtccca caaaactacc aagacgactc 120

agatcaatac caacagaatt ctgtccaaca aaacccaccg caacatcagg aaaaatcgca 180

atatgaacca cgagtaaaag aaactatcaa attcatacca attatcagat tcgataaaga 240

acaaggagaa gccggtaatt acaaagctgc atatgaaacc ggaaacaaca ttatcgctga 300

agaaggaggt tacttgaagg aactcggtcc agatccagac tagcaaggac aagttatcaa 360

atcgcaagta caacagggtc acttcacata tacatcccct gaaggagtag ttattgaaac 420

taaatatact gctgacgaaa acggtttcca tgttgaagga gatcatttgc caaccccgcc 480

accagttagc gccgaagtac aaaagggatt ggaccttatt tttgctggta tcagagctca 540

acaggaggcg ttagaacaaa gattaaaaga tcacccagaa gaaagagaca gacctgaaaa 600

ttacgacgat ggtcaatacc gaccggcaca acaggagtaa ttttactctt tatttatagt 660

ttagcaccat cattctgtga ttttgtcgtc 690

<210>343

<211>633

<212>DNA

<213>Diabrotica virgifera

<400>343

caacactatg ttctctaaaa ttttgtcagt gttagccttc acagctttag cacaagctgg 60

cgttttagac tacagtcacg gccatgctac ctcatatgct tcctcaaacc taggtggttc 120

agaacatgga catggatatt cagctccagt tttaagtcac gcatatgctg cacctgttgc 180

tcacggatat tctgctcctg ttctcgccca cggacattcc gcacatcacg atgtagatta 240

caacgcttat cccaaatacg aattcaacta cggagttcaa gatggccaca ctggagacca 300

caaaactcaa cacgaagtcc gtgatggtga cgtagtcaaa ggttcctaca cagtagctga 360

acctgacggt actcttcgta ctgtccacta cactgctgat gaccacaacg gtttcaatgc 420

tgttgttgaa aaatctggac acccagttca cccagctcca gctcatggac acggaaaagt 480

actcctagct gctcccgctg tccaccacgc tccattgtac catcattaaa acctgaaaga 540

tgcatgttga ttattgttac tgtatttatt gactttgttg attgaattac tatgtaatta 600

atttttgtac ataaaataaa tttaattgaa tat 633

<210>344

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>344

ctaggtggtt cagaacatgg acatgga 27

<210>345

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>345

caggttttaa tgatggtaca atggagcgtg 30

<210>346

<211>421

<212>DNA

<213>Diabrotica virgifera

<400>346

ctaggtggtt cagaacatgg acatggatat tcagctccag ttttaagtca cgcatatgct 60

gcacctgttg ctcacggata ttctgctcct gttctcgccc acggacattc cgcacatcac 120

gatgtagatt acaacgctta tcccaaatac gaattcaact acggagttca agatggccac 180

actggagacc acaaaactca acacgaagtc cgtgatggtg acgtagtcaa aggttcctac 240

acagtagctg aacctgacgg tactcttcgt actgtccact acactgctga tgaccacaac 300

ggtttcaatg ctgttgttga aaaatctgga cacccagttc acccagctcc agctcatgga 360

cacggaaaag tactcctagc tgctcccgct gtccaccacg ctccattgta ccatcaaaac 420

c 421

<210>347

<211>698

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(14)

＜223〉n=a, c, g or t/u

<400>347

aacatggcat tcanattagt tgctttcgcc gcattggtag cagtagctcg tgctggtgtc 60

atcggagaag cctactctgc accacttgcc tatgcagctc cagcagtagc taaggtagcc 120

tactcagccg ctccagctgt atcatactca agtgtgtcat cacctgtagc ttacgcagca 180

ccagctgttg ctaaattagc tgcacctgta tcttatgctg caccagcttt atcttacgct 240

gcaccagtcg ctaaagttgc ctactcatca gctccagctg tctcttactc cagtgtatcc 300

gcaccagtag cctacgccgc tgccccagcc tacgccaaag tagctgctcc agtatcgtac 360

gcagctccag ctgtttccta cgccgctcca gctctctcct atgccgcccc agcccttaaa 420

gtagctgccc cagtagctta tgccgctcct gttgccaaag cagtttacgc cgaaccatca 480

gcaccagcta actacgattt tggatatgcc gtaaacgacc cccacaccgg agatagcaaa 540

agccaacaag aatctcgcca cggtgatgtt gtacatggaa gctattccct tattgaagcc 600

gatggcagca aacgtaccgt agaatacacc gctgatgaac acaatggttt taatgcagtt 660

gtacacaagg aaccagccac agttgcggtt aaagcagt 698

<210>348

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>348

gctcgtgctg gtgtcatcgg agaag 25

<210>349

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>349

gcaactgtgg ctggttcctt gtgtac 26

<210>350

<211>641

<212>DNA

<213>Diabrotica virgifera

<400>350

gctcgtgctg gtgtcatcgg agaagcctac tctgcaccac ttgcctatgc agctccagca 60

gtagctaagg tagcctactc agccgctcca gctgtatcat actcaagtgt gtcatcacct 120

gtagcttacg cagcaccagc tgttgctaaa ttagctgcac ctgtatctta tgctgcacca 180

gctttatctt acgctgcacc agtcgctaaa gttgcctact catcagctcc agctgtctct 240

tactccagtg tatccgcacc agtagcctac gccgctgccc cagcctacgc caaagtagct 300

gctccagtat cgtacgcagc tccagctgtt tcctacgccg ctccagctct ctcctatgcc 360

gccccagccc ttaaagtagc tgccccagta gcttatgccg ctcctgttgc caaagcagtt 420

tacgccgaac catcagcacc agctaactac gattttggat atgccgtaaa cgacccccac 480

accggagata gcaaaagcca acaagaatct cgccacggtg atgttgtaca tggaagctat 540

tcccttattg aagccgatgg catcaaacgt accgtagaat acaccgctga tgaacacaat 600

ggttttaatg cagttgtaca caaggaacca gccacagttg c 641

<210>351

<211>977

<212>DNA

<213>Diabrotica virgifera

<400>351

attggaaata tagcaacatg gccgtcaaga ttaccttcag catccttata gcagttctat 60

tagccagaaa cagggtcgat gcctcaggag ttgcaccttt atttgctcca gcaccagtat 120

tagccccagc accagttcta gctccagctc cagttttggc caaagtttct gatgccacaa 180

ttgaccccaa tcctgcatat tcatttgcgt atgacgtgca ggatgcccta acaggagatt 240

caaaaggaca agttgaaagc agagccaacg gcgttgtaag aggacaatat caagtagcag 300

atccagacgg taccagaaga attgtagatt attacgccga tcctgtgcat ggattcaatg 360

ctgtggtcag aagagttcct ctaggtgttg cagttgctcc tgtcgtagcc aaagctgtag 420

ctcctgttgc tccaatcgct cccgtcgcta gatcattagt ccttcctgct ccagggcttc 480

cagttgctgc cgctcctgta ccagtagctc cagcattacc tttcgccgca ccagcagtag 540

caccagctct tccatttgcc gctgctccag ctgtagcacc agctcttcca ttcgcagctc 600

cagctgtagc accagcattg ccgtatgctg cagctccagc tatttctcca tacttaacca 660

gagctggatt cttcccagct ccagctccag ccgtagttcc tgctgctcca ggtcctgcat 720

acttgcctgc tgctccatca agattggttt caccctattc ggtagccgca cccttgcctt 780

caggtttgag gagtttagtg acccccttct cagcgggtcc tgctccacta gccgttggat 840

caggggtagc ggcgccgtat tggtaaaccc cggccgtgta ctaaatattg actgactata 900

tattttttaa gtagtttaca tttgttttat tgtttctagt atttttattg taaataaata 960

tcctatattt ttatctt 977

<210>352

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>352

caagattacc ttcagcatcc ttatagcagt tc 32

<210>353

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>353

gtcactaaac tcctcaaacc tgaaggcaag 30

<210>354

<211>777

<212>DNA

<213>Diabrotica virgifera

<400>354

caagattacc ttcagcatcc ttatagcagt tctattagcc agaaacaggg tcgatgcctc 60

aggagttgca cctttatttg ctccagcacc agtattagcc ccagcaccag ttctagctcc 120

agctccagtt ttggccaaag tttctgatgc cacaattgac cccaatcctg catattcatt 180

tgcgtatgac gtgcaggatg ccctaacagg agattcaaaa ggacaagttg aaagcagagc 240

caacggcgtt gtaagaggac aatatcaagt agcagatcca gacggtacca gaagaattgt 300

agattattac gccgatcctg tgcatggatt caatgctgtg gtcagaagag ttcctctagg 360

tgttgcagtt gctcctgtcg tagccaaagc tgtagctcct gttgctccaa tcgctcccgt 420

cgctagatca ttagtccttc ctgctccagg gcttccagtt gctgccgctc ctgtaccagt 480

agctccagca ttacctttcg ccgcaccagc agtagcacca gctcttccat ttgccgctgc 540

tccagctgta gcaccagctc ttccattcgc agctccagct gtagcaccag cattgccgta 600

tgctgcagct ccagctattt ctccatactt aaccagagct ggattcttcc cagctccagc 660

tccagccgta gttcctgctg ctccaggtcc tgcatacttg cctgctgctc catcaagatt 720

ggtttcaccc tattcggtag ccgcaccctt gccttcaggt ttgaggagtt tagtgac 777

<210>355

<211>1176

<212>DNA

<213>Diabrotica virgifera

<400>355

aaatctatca aacttttatt tatttagatt caaaatacga ttatttatac attaacgtta 60

ttaatccatg ggtattcgtc tggacaagga acacacgcgt ccatgtttct atcctctgtt 120

ccttttttaa gtctgcaggt gtaaggctca caactggctc catcgggcgc gtcagcagtt 180

ttaaaatcgc taattttaac gggatttttt ataaaatcta ggacctgttt ttgtgagacg 240

aagaaggtat cttttgcttc acgtgcaact gtgttttcca agaattttcg aagcccatcg 300

aagctggtgt cagtttgttc aaaccagtat gagttgatca tcaaatttat gggagttcta 360

gatttacttc taattccttc aatttgatca tccaaatatt tggcgatttc atcaacacta 420

ccttctattc cacaagtagc cagtgaattg cattctgtac catttggtga agttaaatcg 480

tttattggca gaatccaata acctgggaaa gcttcgttag gacattctgc gcccaaaaga 540

cattgttctg ttgattgata atccaatgta tagggaaata atcttcttga tggcaaagag 600

ggccatgaac tatcatatgc caattctgat gctatatagg cctcaatgga agtgtttcct 660

gcaagttgta gttggggagt tctgactcca ataatagctt cagctggtat gttggcataa 720

gtacttataa tttgcttttg gcctttaaat tcttttgtta gtgtgtctag agttgcatat 780

ctccagtaat tttgtctaaa atccttcgta attgagtgca caccaatttc aaatccaaag 840

ttgtacaaat cattaactct ttgatagctc gtatactcat gtggtacaaa gaatgttcca 900

ctaattggct tatcatctgg attatatata tttccgagca atgtaatcca actgttgtcg 960

tagatgtgtt gtgtaacagc ttcatcaaat gttaaactaa tcagctgagg atactctgtg 1020

agatctccac ctagaggtga cacttgacta gaacatctac aattgttgcc gatcgtacaa 1080

gaatcagact cacacggttt tgctacatca tttgcaatcg cactatcgaa tgctaaagta 1140

gctacgagaa ctaaaatcaa gcactggaac gttgcc 1176

<210>356

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>356

gcaaatgatg tagcaaaacc gtgtgag 27

<210>357

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>357

ggtattcgtc tggacaagga acacacg 27

<210>358

<211>1043

<212>DNA

<213>Diabrotica virgifera

<400>358

ggatcgtctg gacaaggaac acacgcgtcc atgtttctat cctctgttcc ttttttaagt 60

ctgcaggtgt aaggctcaca actggctcca tcgggcgcgt cagcagtttt aaaatcgcta 120

attttaacgg gattttttat aaaatctagg acctgttttt gtgagacgaa gaaggtatct 180

tttgcttcac gtgcaactgt gttttccaag aattttcgaa gcccatcgaa gctggtgtca 240

gtttgttcaa accagtatga gttgatcatc aaatttatgg gagttctaga tttacttcta 300

attccttcaa tttgatcatc caaatatttg gcgatttcat caacactacc ttctattcca 360

caagtagcca gtgaattgca ttctgtacca tttggtgaag ttaaatcatt tattggcaga 420

atccaataac ctgggaaagc ttcgttagga cattctgcgc ccaaaagaca ttgttctgtt 480

gattgataat ccaatgtata gggaaataat cttcttgatg gcaaagaggg ccatgaacta 540

tcatatgcca attctgatgc tatataggcc tcaatggaag tgtttcctgc aagttgtagt 600

tggggagttc tgactccaat aatagcttca gctggtatgt tggcataagt acttataatt 660

tgcttttggc ctttaaattc ttttgttagt gtgtctagag ttgcatatct ccagtaattt 720

tgtctaaaat ccttcgtaat tgagtgcaca ccaatttcaa atccaaagtt gtacaaatca 780

ttaactcttt gatagctcgt atactcatgt ggtacaaaga atgttccact aattggctta 840

tcatctggat tatatatatt tccgagcaat gtaatccaac tgttgtcgta gatgtgttgt 900

gtaacagctt catcaaatgt taaactaatc agctgaggat actctgtgag atctccacct 960

agaggtgaca cttgactaga acatctacaa ttgttgccga tcgtacaaga atcagactca 1020

cacggttttg ctacatcatt tgc 1043

<210>359

<211>649

<212>DNA

<213>Diabrotica virgifera

<400>359

ctattattca gatgtgccac aggtgaaaat tggcctagca taatgttatc gtgtgtgaaa 60

ggcaagatgt gtgacgaaaa ttcgggaaaa gacggacacc agtgtggtca caatatagct 120

tatgcctatt ttgtgtcatt tatattcttc tgttcgttct tgatgttaaa cttgttcgta 180

gccgtcatta tggacaattt tgattattta acacgtgatc cttccatatt gggtgctcat 240

catttagacg aattcgttag aatttgggca gaatacgatc caaatgccac aggaaaaatc 300

cactacagcg aaatgtacga catgttgaaa aatatggatc cgccactagg ctttggaaac 360

aagtgtccta atcgattagc gtataagaaa ttaattagga tgaacatgcc agtagacgac 420

gatgggaaag ttaattttac tacgacattc tttgctctcg tcagagaaaa tcttagcatt 480

aaaatgcgaa cagctgatga aatggaccag gccgatgacg aactcaaaga gacaatcaaa 540

aacatctggc cgatccaagc caagaacatg gtggagctgt tggttccacc gaccaaccag 600

ctcaccaccg gaaaatgtcc gtcggcaagt tatacgccgg tcttctgat 649

<210>360

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>360

ctattattca gatgtgccac aggtgaaaat tg 32

<210>361

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>361

cagatgtttt tgattgtctc tttgagttcg 30

<210>362

<211>547

<212>DNA

<213>Diabrotica virgifera

<400>362

ctattattca gatgtgccac aggtgaaaat tggcctagca taatgttatc gtgtgtgaaa 60

ggcaagatgt gtgacgaaaa ttcgggaaaa gacggacacc agtgtggtca caatatagct 120

tatgcctatt ttgtgtcatt tatattcttc tgttcgttct tgatgttaaa cttgttcgta 180

gccgtcatta tggacaattt tgattattta acacgtgatc cttccatatt gggtgctcat 240

catttagacg aattcgttag aatttgggca gaatacgatc caaatgccac aggaaaaatc 300

cactacagcg aaatgtacga catgttgaaa atatggatcc gccactaggc tttggaaaca 360

agtgtcctaa tcgattagcg tataagaaat taattaggat gaacatgcca gtagacgacg 420

atgggaaagt taattttact acgacattct ttgctctcgt cagagaaaat cttagcatta 480

aaatgcgaac agctgatgaa atggaccagg ccgatgacga actcaaagag acaatcaaaa 540

acatctg 547

<210>363

<211>677

<212>DNA

<213>Diabrotica virgifera

<400>363

caataggtat ggcctctgcg aaagatacgg ctacgttctt tactcacggc acctgtcaac 60

atttcgagca ggtcctcaag ttatatccac aagcccttcg attgaaagct gatagaaaag 120

ccaaaaagcc cgaggaactg attaaattag acaattggta tcagaatgaa ctaccgaaaa 180

ggatcaaaag tcgtggcaaa gatgcccact tagtacacga ggagttggta caatgcatga 240

aatggaaaca gaccaggggg aagttcttcc cccaattatc gtatttggta aaggttaaca 300

ctcctagagc tgttatggca gaaacgaaga gagcctttag aaaattgccc aatcttgagt 360

tggcgatgtc agcgctctca aatttgaaag gagtcggtac cacaatggcc tcagcattac 420

tggcggctgc ttgtccagac agcgccccct ttatggcaga tgaatgtctg atggccatac 480

cagacatcga aggtattgat tacaccacca aggagtacct gaaattcgcc aaccacatca 540

aatccgtcgt cgagagatta aacaaggccg cagaagaaag gtcagaggct gtacgttggt 600

cgccacacaa ggacgagtta gctctttgga cacctacgtt gtctccgaac acaaaccaga 660

gttgctctct agtattt 677

<210>364

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>364

gttctttact cacggcacct gtcaaca 27

<210>365

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>365

ctaactcgtc cttgtgtggc gacc 24

<210>366

<211>587

<212>DNA

<213>Diabrotica virgifera

<400>366

gttctttact cacggcacct gtcaacattt cgagcaggtc ctcaagttat atccacaagc 60

ccttcgattg aaagctgata gaaaagccaa aaagcccgag gaactgatta aattagacaa 120

ttggtatcag aatgaactac cgaaaaggat caaaagtcgt ggcaaagatg cccacttagt 180

acacgaggag ttggtacaat gcatgaaatg gaaacagacc agggggaagt tcttccccca 240

attatcgtat ttggtaaagg ttaacactcc tagagctgtt atggcagaaa cgaagagagc 300

ctttagaaaa ttgcccaatc ttgagttggc gatgtcagcg ctctcaaatt tgaaaggagt 360

cggtaccaca atggcctcag cattactggc ggctgcttgt ccagacagcg ccccctttat 420

ggcagatgaa tgtctgatgg ccataccaga catcgaaggt attgattaca ccaccaagga 480

gtacctgaaa ttcgccaacc acatcaaatc cgtcgccgag agattaaaca aggccgcaga 540

agaaaggtca gaggctgtac gttggtcgcc acacaaggac gagttag 587

<210>367

<211>882

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(792)..853)

＜223〉n=a, c, g or t/u

<400>367

ggagaaacta acatcgttga caaccatggc ggataaagaa aagaaggtca agaagaagaa 60

gaaggaagag ggcgaagcac ctgctgccgc tcctgccccc gccgaaacca ggtcctcctc 120

aaagagttcc tccaagaaag ccaaaaggtc tggttccaat gtcttctcca tgttttcgca 180

atctcaagta gctgaattca aagaagcttt ccaacttatg gaccacgaca aagatggcat 240

catctctaag agcgatttga gggccacctt cgatgctgtt ggtaaactcg ccagtgagaa 300

agagctcgac gagatgatca acgaagcacc cggaccaatc aacttcactc aattgttggg 360

attgttcggt acccgtatgg ctgattccgg cggtactgac gatgatgaag ttgtcgtcaa 420

ggctttcaga tcctttgacg aaaacggcac cattgacgga gacagattcc gtcatgccct 480

catgacctgg ggagaaaaat tcaccggcaa agaatgcgac gacgccttcg atgccatgga 540

catcgacgac aacaacagga tcgataccca ggcacttatc accctcttaa cagcagccga 600

gaaggaagaa ggtgaagaag gcgaagcagc ataaacacca tcataactta taacagaaag 660

tcaatgtaaa tcttcacggc taccgtgtgg ttccactaag acgaaaacat caattcggat 720

acatctaagt gtggtgtgtt tcttatataa ttccaatgaa cacataagaa aaggtgtact 780

tatatgtgta tnttattatg tatttgtttt taagtttatt tttattcagt tgctttacca 840

tcacgcgact canaagaaat aatatattat tttttgtttc at 882

<210>368

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>368

ggcggataaa gaaaagaagg tcaagaag 28

<210>369

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>369

cacaccacac ttagatgtat ccgaattgat g 31

<210>370

<211>711

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(631)

＜223〉n=a, c, g or t/u

<400>370

ggcggataaa gaaaagaagg tcaagaagaa gaagaaggaa gagggcgaag caccagctgc 60

cgctcctgcc cccgccgaaa ccaggtcctc ctcaaagagt tcctccaaga aagccaaaag 120

gtctggttcc aatgacttct ccatgttttc gcaatctcaa gtagctgaat tcaaagaagc 180

tttccaactt atggaccacg acaaagatgg catcatctct aagagcgatt tgagggccac 240

cttcgatgct gttggtaaac tcgccagtga gaaagagctc gacgagatga tcaacgaagc 300

acccggacca atcaacttca ctcaattgtt gggattgttc ggtacccgta tggctgattc 360

cggcggtact gacgatgatg aagtcgtcgt caaggctttc agatcctttg acgaaaacgg 420

caccattgac ggagacagat tccgccatgc cctcatgacc tggggagaaa aattcaccgg 480

caaagaatgc gacgacgctt tcgatgccat ggacatcgac gacaacaaca ggatcgacac 540

ccaggcactt atcaccctct taacagcagc cgagaaggaa gaaggtgaag aaggcgaagc 600

agcataaaca ccatcataac ttacaactga nagtcaatgt aaatcttcac ggctaccgtg 660

tggttccact aagacgaaaa catcaattcg gatacatcta agtgtggtgt g 711

<210>371

<211>2918

<212>DNA

<213>Diabrotica virgifera

<400>371

gtagagttga ggaagatgtg caaagtgttg aaaagtttat gtacatgaat tgtagaaact 60

aattgtttat tgtgtatcat tcgaattcag tttcaaaatt aaaagtgtgc tagtggagaa 120

aactaaacga taacttctgt ttatactgtt gtttttatcg tttaacaatg gctctctttc 180

ttctaggttt gttgttctgc ctatctagtc ggatctctgc cagtggtttc tacgaaatca 240

gtaacattga cttctataat tctgttttta cagattctct tgattggaga ttgttacagg 300

agctatcaaa ccagtataaa aacgtagtga tatctcccat cagcctaaaa atcatattat 360

ccctattata tcaaggatct accggacaga cagaaaggga attccaaaca cttttaaatt 420

atcaaaataa agaatatgtc aggaacaatt acagccaaat tgtcgctgcc ctttataatg 480

cagacagaac tgaatatatg ctcaacattg ggacatcgat gtttgtagat gaaggtctat 540

acgttttatc taaattcgaa gaccttgcaa gaaaaagctt caaaacagac atcataaaga 600

ctaactttga caaacaggaa aaggctagta aagatattaa ttcgtgggtg gaacagctta 660

ctcatggtaa agtgaagaaa atagtcaatg ctgatgatct gtcgcaagca ctaatgataa 720

ttaccaatgc catttacttc aaaggaacat ggactcgtaa atttgataaa aatagatcaa 780

cgcttggaaa cttctataca tctcctgata atataagatc tagcagaaac ttaaaattag 840

ttcaatacat gactaccact gacgaatttc tttattacga ggacagatct ttggatgcga 900

aaattgtcag gttggaatac aagggaagtg actatgccat gtatatagtc ctaccgaaca 960

ctttgggagg attaagcagc cttatcaaaa atgtggacat aaacaaaata ggaactataa 1020

agttccaaat gacacaacaa gttgttgagg ttactattcc aaagtttaag ttcaattttg 1080

acatcaagtt gggaaagatt cttcaaaagt ttggactacg cgaagcattc caaaacacgg 1140

ccagttttac aggcatcgtg caaaccaaca ggaccttaag aaggagtcta tacgtatctg 1200

acattgttca gaaatcggga atagaagttg atgaagaagg aagtgaaatc ttctcagcat 1260

cagctgtcaa cgtgggtaat aaattcgccg aaaatacgat gatattcaat gcatcacatc 1320

cgttcatgtt ctttgttgat ggtcccaacg gtaccatatt gtttgttgga caatatgaaa 1380

atccagatgc ggtggacccg tcagaggtac cgaatcgttg gagtcaagaa gtagaagaaa 1440

aaaatcctac tgaagctgtg caattccaaa atactcgccc aagtagacca aataatgaaa 1500

ttgatcaatc caataatgta cagttccaac agcctcgtcc aaatagacca agtgttgaaa 1560

gtcaacgacc cgacaatgta caattccaac agaatcgccc aaatagacca aatactgaaa 1620

atgaacagcc cagcaatgta caaggtctta atttgaatcc aaatctggac cccgactact 1680

atcaacctcc agatgtagaa atggatgaga ttgcatatag attcaatctg tttgatattg 1740

acttattata ttccttttct gatttatcca cgaacgtatt aatatctccc gctagcatca 1800

aaacgacatt agcaatgatt ttagaaggag cagaaggaac ttgtgcagaa gaaataagtg 1860

aagcactgag gatacctgat attaatcaaa aaggtgtcag aagtgttctt gtagaacttc 1920

taaataatct taatgaaaga tctacgccaa atagtgtcct agaaagccat aatgccatat 1980

ttgtctctga aaaacatcga ctggttgata attataagaa tgtcgttata aagtattaca 2040

acgcttacct gaaaagctta gatttttcaa attcagatta tgctgctagg gtcattaatg 2100

gatggatatc tgactccact catggcgaaa taaccgatgt catctcacca caggggatag 2160

ttcctgattg ttacgtggtg ctgtcaaacg ccctgttctt taaagcacag tggaaatatg 2220

cttttgataa aagaagtgta cgaaaatgtt tccacactcc taggggatgt gtttatgttg 2280

acatgatgca catttcacat aatttcaatt acaattacat cacccgtttg cgagccaatg 2340

ttgtggatat tccttatcag gataaattct ccatgctact gatcttacca tcttcagatt 2400

caaatgtaag gagtgtgatt cgggatttac ctcactttag actgtcgagc attttggaga 2460

gtctaaatca atccgagatt gttctagaac taccaacatt tagcttcgag tattcagcag 2520

atttggttga atttttaaaa cctcttaaaa tccgagaaat atttggttcc cgcgctaact 2580

taaccaaaat gatagaaggc caccatggta tgataaacaa cctgttacac aagaccaaaa 2640

tcacagtaga cgaaaaagga acaactgccg cagcatcctc cagtgccatg ataattcccc 2700

tgatgcagcc agtgacagtt gcggctgaca gaccgtttgt ttttatgatc tatcacagag 2760

acactaaaaa tataatcttt gaaggaattg tacaaaatcc tttagaaaaa tagtatctaa 2820

cattactgat aattttttat ttaatgtgcc gaaaactcat ctaggttaga tctcttcaaa 2880

ctgtagttac gtgtaaaaat tggtctgtat ttgctttt 2918

<210>372

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>372

ctagtcggat ctctgccagt ggtttc 26

<210>373

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>373

ccttcttcat caacttctat tcccgatttc 30

<210>374

<211>1036

<212>DNA

<213>Diabrotica virgifera

<400>374

ctagtcggat ctctgccagt ggtttctacg aaatcagtaa cattgacttc tataattctg 60

tttttacaga ttctcttgat tggagattgt tacaggagct atcaaaccag tataaaaacg 120

tagtgatatc tcccatcagc ctaaaaatca tattatccct attatatcaa ggatctaccg 180

gacagacaga aagggaattc caaacacttt taaattatca aaataaagaa tatgtcagga 240

acaattacag ccaaattgtc gctgcccttt ataatgcaga cagaactgaa tatatgctca 300

acattgggac atcgatgttt gtagatgaag gtctatacgt tttatctaaa ttcgaagacc 360

ttgcaagaaa aagcttcaaa acagacatca taaagactaa ctttgacaaa caggaaaagg 420

ctagtaaaga tattaattcg tgggtggaac agcttactca tggtaaagtg aagaaaatag 480

tcaatgctga tgatctgtcg ctagcactaa tgataattac caatgccatt tacttcaaag 540

gaacatggac tcgtaaattt gataaaaata gatcaacgct tggaaacttc tatacatctc 600

ctgataatat aagatctagc agaaacttaa aattagttca atacatgact accactgacg 660

aatttcttta ttacgaggac agatctttgg atgcgaaaat tgtcaggttg gaatacaagg 720

gaagtgacta tgccatgtat atagtcctac cgaacacttt gggaggatta agcagcctta 780

tcaaaaatgt ggacataaac aaaataggaa ctataaagtt ccaaatgaca caacaagttg 840

ttgaggttac tattccaaag tttaagttca attttgacat caagttggga aagattcttc 900

aaaagtttgg actacgcgaa gcattccaaa acacggccag ttttacaggc atcgtgcaaa 960

ccaacaggac cttaagaagg agtctatacg tatctgacat tgttcagaaa tcgggaatag 1020

aagttgatga agaagg 1036

<210>375

<211>620

<212>DNA

<213>Diabrotica virgifera

<400>375

gatcggcgct cgactagtag tcggagtagg gcggccaaag taattttttg attattatag 60

ttaattggtt tattttaaat taggtattaa gaaaaaatca aaatatggct attaatctac 120

aatatgacgc cataggcaaa agttttgtcc aacaatatta tatgcttttc gatgatccaa 180

accaaaggcc taatttggta aacatgtata atgtagaaca ttcatttatg acctttgaag 240

gcaagcaatt acaaggatct gcaaagataa tggagaaatt aaattcctta ggattccaaa 300

atataaatag acaacttact gaagtagact ctcaacctat gtttgatggt ggtgtactga 360

taaatgtatt aggtagattg caagttgata atgatccacc acacgcttat acacaagttt 420

ttgttttaaa accattaaat ggatcatttt ttgttcaaca tgacattttt aggctgaaca 480

tacacaatag tgcctgattt tattacatct cctctccttc tttatgttgt atgttcgaat 540

caactgaaaa tatgcaattt ttaaaatctc ctgtatctgt ttctgcttaa tatgaattca 600

caataataaa cttattttct 620

<210>376

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>376

ctattaatct acaatatgac gccataggca aaag 34

<210>377

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>377

cgaacataca acataaagaa ggagaggaga tg 32

<210>378

<211>429

<212>DNA

<213>Diabrotica virgifera

<400>378

ctattaatct acaatatgac gccataggca aaagttttgt ccaacaatat tatatgcttt 60

tcgatgatcc aaaccaaagg cctaatttgg taaacatgta taatgtagaa cattcattta 120

tgacctttga aggcaagcaa ttacaaggat ctgcaaagat aatggagaaa ttaaattcct 180

taggattcca aaatataaat agacaactta ctgaagtaga ctctcaacct atgtttgatg 240

gtggtgtact gataaatgta ttaggtagat tgcaagttga taatgatcca ccacacgctt 300

atacacaagt ttttgtttta aaaccattaa atggatcatt ttttgttcaa catgacattt 360

ttaggctgaa catacacaat agtgcctgat tttattacat ctcctctcct tctttatgtt 420

gtatgttcg 429

<210>379

<211>846

<212>DNA

<213>Diabrotica virgifera

<400>379

agctattttt tatgtttatt ttttgttatt ataatttaca attaatttcc gttgcagtat 60

tcaaagtttt attaaaacat ttatcgtaaa tttagtgtta ttgttcctca agcagaattt 120

ttatagaaga aaacatggaa actcccggcg ccagcagagc cccttccaaa ctggagcaga 180

cccagcaaca ggtgaatgaa gttgtgggta ttatgagagt aaacgtcgag aaagttctag 240

agagggacca gaaactttca gagctcgata gtcgagctga tgcccttcaa gaaggcggca 300

aaaggttcga gcagcaagca caaaagttga aacgaaagta ttggtggaaa aatataaaga 360

tgatgattat tatgggggtt ataggcctca ttattttaat tatcattata atttccattg 420

ctaatgcagt taagggcgac gacaccacat cctcaagcgt aacgtcggct cctccaaaat 480

gaaattaaat gtttacggat ctttctgtat ttgattatat attctgtatt tgattatata 540

tatttattgt tgcattatac ttaaaaataa gaacaaaata ttttacaatt ctatgtttgc 600

tttatgtcat tattttaaat atattattcc atagaatatg gtgtttctgt ttaaaaagtg 660

aacacctatc agttgtgtaa gtattgagaa tttgtttttc attattatgg gcataggcgc 720

aaaatgtcgc ctgtcaaaat gttcaatgtg ttttaaatgt attcattttt tttttcgaat 780

cctgagaaaa ctaataaata tttttgaaaa atataaacgc agaatgaaag attgcattat 840

tactga 846

<210>380

<211>35

<212>DNA

<213>Diabrotica virgifera

<400>380

gtgttattgt tcctcaagca gaatttttat agaag 35

<210>381

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>381

cacattgaac attttgacag gcgaca 26

<210>382

<211>656

<212>DNA

<213>Diabrotica virgifera

<400>382

gtgttattgt tcctcaagca gaatttttat agaagaaaac atggaaactc ccggcgccag 60

cagagcccct tccaaactgg agcagaccca gcaacaggtg aatgaagttg tgggtattat 120

gagagtaaac gtcgagaaag ttctagagag ggaccagaaa ctttcagagc tcgatagtcg 180

agctgatgcc cttcaagaag gcggcaaaag gttcgagcag caagcacaaa agttgaaacg 240

aaagtattgg tggaaaaata taaagatgat gattattatg ggggttatag gcctcattat 300

tttaattatc attataattt ccattgctaa tgcagttaag ggcgacgaca ccacatcctc 360

aagcgtaacg tcggctcctc caaaatgaaa ttaaatgttt acggatcttt ctgtatttga 420

ttatatattc tgtatttgat tatatatatt tattgttgca ttatacttaa aaataagaac 480

aaaatatttt acaattctat gtttgcttta tgtcattatt ttaaatatat tattccatag 540

aatatggtgt ttctgtttaa aaagtgaaca cctatcagtt gtgtaagtat tgagaatttg 600

tttttcatta ttatgggcat aggcgcaaaa tgtcgcctgt caaaatgttc aatgtg 656

<210>383

<211>841

<212>DNA

<213>Diabrotica virgifera

<400>383

gtgaatcatg tgtttaatta agttatagta tttattctat attaaattac gtagaatata 60

atctctctgt gttgctttac acattaacta tgtataaaaa tgttacaaag gatgattggt 120

ccaaggagaa tacagagaat cgttttggca acctttatat tttccgttct acatatattt 180

ggagtttttc atcatatttt tgagactgat ttttatacaa actttcatta tccctacgat 240

ggggatatag aacatttaat acagcaattg aaggatggca agccacctga tgttttacct 300

attaattcct atcactttga atataacaag aaatgtgttt caaaatgttc cagtatgaaa 360

cagttaagat tggtttatat aataaagagt gctccagagc attttgatcg cagaattgga 420

atacgcaatt catgggggta tgaaaggcga ttttctgatg cagaaataag aactgtcttt 480

ttggttggag aacgaaattc acatgcttta aaagagtcat taaaagaaga atactcaaag 540

tttcatgata ttgtccatgc caattttaca gatagttact acaacaatac ttacaaaact 600

atgatgggct tgcagtgggc tgtcaggtat tgtccaaatt cacaatatta tatgtttgtt 660

gatgatgatt actacgtgtc caccaaaaat gttttaaagg atttgaaaat cccacaagta 720

ttcagaatat gtaaagaacc tcttaatgga gttagtccac tttttaataa aaaaaaaatc 780

ttgtttataa tgatttaaat gaggatgaaa aattattttc aggttatgta tttccatccc 840

c 841

<210>384

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>384

ggagaataca gagaatcgtt ttggcaac 28

<210>385

<211>36

<212>DNA

<213>Diabrotica virgifera

<400>385

ggactaactc cattaagagg ttctttacat attctg 36

<210>386

<211>636

<212>DNA

<213>Diabrotica virgifera

<400>386

ggagaataca gagaatcgtt ttggcaacct ttatattttc cgttctacat atatttggag 60

tttttcatca tatttttgag actgattttt atacaaactt tcattatccc tacgatgggg 120

atatagaaca tttaatacag caattgaagg atggcaagcc acctgatgtt ttacctatta 180

attcctatca ctttgaatat aacaagaaat gtttttcaaa atgttccagt atgaaacagt 240

taagattggt ttatataata aagagtgctc cagagcattt tgatcgcaga attggaatac 300

gcaattcatg ggggtatgaa aggcgatttt ctgatgcaga aataagaact gtctttttgg 360

ttggagaacg aaattcacat gctttaaaag agtcattaaa agaagaatac tcaaagtttc 420

atgatattgt ccatgccaat tttacagata gttactacaa caatacttac aaaactatga 480

tgggcttgca gtgggctgtc aggtattgtc caaattcaca atattatatg tttgttgatg 540

atgattacta cgtgtccaca aaaaatgttt taaggtattt gaaacatccc acaaagtatc 600

cagaatatgt aaagaacctc ttaatggagt tagtcc 636

<210>387

<211>2158

<212>DNA

<213>Diabrotica virgifera

<400>387

gccattgggc gatagtctct ccatggaaaa ttaatatttg gaagaaagta tccataagta 60

atattctgtc aggttgaatg gagctagtat ctagtaatac aggctatgat ggaccattga 120

aactataact atacaaatta ggttgaatca ttatcaaaga ttgagtaaga tcttccctca 180

tcaacatgtg tctgtagaat gaagtctcgt ccggagaatt attgaatact tgaagaaatt 240

gagatcttct taagtgatac atgaactgtg ggtaaagact gaagttttga ccaagtctga 300

atgaattggg gtcgtcctta ttgtattctc cgaatttttg acacaatcta atcagcattc 360

tgtcaaccca tctaagaaca tctggactat catcagattc tgccctataa acggccatcc 420

tagccattat tacagcagca gcttcttgat cgaatccggc actgatatgg tgtatattag 480

cagttgcgtc agcccaattt cgagccactg ttgttactct gatttttctt tgaccacttg 540

aatgctggta ctgcgtaata aattgtatac aacctctacc accttgaggt atgggagcag 600

aatgttgatt tacgacctca aagaataaag acatggtagt acttggcgtt aaagtacaca 660

ttttccattg cacagtatta cccattccta tttctgtgtc ggaaaccaag gggctcttca 720

cgttaagtga tacacacgaa ccgatacctc cttgaacttt taattctcgg gaacacttca 780

cttccaaagt accgttaaat gccattttca gatcactttt ttgatctctg gtaaacacac 840

gttggaaagt ttgcttaaac aaggaagaat taaatgaatc ccccattacc atgtgtcccc 900

cagtagaatt acagcattgc ttcatttcca tcagacctgt ctgatccaaa gcacaagaat 960

aaatatcaac agagtgacca ttagttgcgg ctctcattgc taacgcatca taatgtttaa 1020

tacctttctt catatatttt gcattatctt tctgaatatc atgatgtgat ctaataggct 1080

gttttaaatc atcattaact acctgaccag gtccttgtga gcaaggtcct ccaacaaata 1140

gcataactcg ggcgccagta ttggaatatg tacattctaa caaaccaatg gcaatcgata 1200

aagctacacc tgtagatctt aaataccttt taccctgatg aacaggccaa ggatctttct 1260

gttgttctcc tattaggtct gttagattca tatcgcattt ggatacaggt tgtagaaacc 1320

tgcttcctgg tgcaacaaca gtaggttgca tttgcccagg tcttagaggc tgccctggct 1380

gttgaggggc ttgctgacct aaagccactt tgcctattcc cagcatttct tgaacctgtt 1440

tagcactaag atcttttgta cctctgaaca catatgactt actacaacct tcagtgccaa 1500

gttcatgaac ttgaaccatt ttcccaaatg ttattagtcc tattaacgca ttaggtggca 1560

acaaactaag ggacatttgc aatgagtctt tcagggaacc cagttcttca tcatccatgc 1620

aggtgtcaac aacatacaaa aatattggtg gtaaacattg agctctagtt attgtgtatt 1680

caatggtgga aaacataggc atcaattccg ctggttgatg ttgttctgaa atagcagcat 1740

attgaggtgg aaatggattt ctctggaaac aaaagttgca tacccagagt tttgctcgat 1800

aatctacctg acacagtggg tttaatattg ctctacaagt atttctagta cataaaacag 1860

ggtcatattg tattggtgga agatccagac gttcctttat aggctggtac agacaagcta 1920

agggtactac gagacgggta gcttcaattc tgcttgaagg ccatacattc caggtaaatc 1980

taatcccatc tcgatcttca ttttgttgta tatactcttc atatgtgctc atttttgaag 2040

attacaagtc ggaattccct aaggaaattc ctcaatatat attattagga ttctatcatg 2100

taaataacac tattattgag ctcctaaaaa ttacacctaa ttgtttcaga atttgaac 2158

<210>388

<211>33

<212>DNA

<213>Diabrotica virgifera

<400>388

gtcactctgt tgatatttat tcttgtgctt tgg 33

<210>389

<211>35

<212>DNA

<213>Diabrotica virgifera

<400>389

ctgtcaggtt gaatggagct agtatctagt aatac 35

<210>390

<211>913

<212>DNA

<213>Diabrotica virgifera

<400>390

gtcactctgt tgatatttat tcttgtgctt tggatcagac aggtctgatg gaaatgaagc 60

aatgctgtaa ttctactggg ggacacatgg taatggggga ttcatttaat tcttccttgt 120

ttaagcaaac tttccaacgt gtgtttacca gagatcaaaa aagtgatctg aaaatggcat 180

ttaacggtac tttggaagtg aagtgttccc gagaattaaa agttcaagga ggtatcggtt 240

cgtgtgtatc acttaacgtg aagagcccct tggtttccga cacagaaata ggaatgggta 300

atactgtgca atggaaaatg tgtactttaa cgccaagtac taccatgtct ttattctttg 360

aggtcgtaaa tcaacattct gctcccatac ctcaaggtgg tagaggttgt atacaattta 420

ttacgcagta ccagcattca agtggtcaaa gaaaaatcag agtaacaaca gtggctcgaa 480

attgggctga cgcaactgct aatatacacc atatcagtgc cggattcgat caagaagctg 540

ctgctgtaat aatggctagg atggccgttt atagggcaga atctgatgat agtccagatg 600

ttcttagatg ggttgacaga atgctgatta gattgtgtca aaaattcgga gaatacaata 660

aggacgaccc caattcattc agacttggtc aaaacttcag tctttaccca cagttcatgt 720

atcacttaag aagatctcaa tttcttcaag tattcaataa ttctccggac gagacttcat 780

tctacagaca catgttgatg agggaagatc ttactcaatc tttgataatg attcaaccta 840

ttttgtatag ttatagtttc aatggtccac cagagcctgt attactagat actagctcca 900

ttcaacctga cag 913

<210>391

<211>2158

<212>DNA

<213>Diabrotica virgifera

<400>391

gccattgggc gatagtctct ccatggaaaa ttaatatttg gaagaaagta tccataagta 60

atattctgtc aggttgaatg gagctagtat ctagtaatac aggctatgat ggaccattga 120

aactataact atacaaatta ggttgaatca ttatcaaaga ttgagtaaga tcttccctca 180

tcaacatgtg tctgtagaat gaagtctcgt ccggagaatt attgaatact tgaagaaatt 240

gagatcttct taagtgatac atgaactgtg ggtaaagact gaagttttga ccaagtctga 300

atgaattggg gtcgtcctta ttgtattctc cgaatttttg acacaatcta atcagcattc 360

tgtcaaccca tctaagaaca tctggactat catcagattc tgccctataa acggccatcc 420

tagccattat tacagcagca gcttcttgat cgaatccggc actgatatgg tgtatattag 480

cagttgcgtc agcccaattt cgagccactg ttgttactct gatttttctt tgaccacttg 540

aatgctggta ctgcgtaata aattgtatac aacctctacc accttgaggt atgggagcag 600

aatgttgatt tacgacctca aagaataaag acatggtagt acttggcgtt aaagtacaca 660

ttttccattg cacagtatta cccattccta tttctgtgtc ggaaaccaag gggctcttca 720

cgttaagtga tacacacgaa ccgatacctc cttgaacttt taattctcgg gaacacttca 780

cttccaaagt accgttaaat gccattttca gatcactttt ttgatctctg gtaaacacac 840

gttggaaagt ttgcttaaac aaggaagaat taaatgaatc ccccattacc atgtgtcccc 900

cagtagaatt acagcattgc ttcatttcca tcagacctgt ctgatccaaa gcacaagaat 960

aaatatcaac agagtgacca ttagttgcgg ctctcattgc taacgcatca taatgtttaa 1020

tacctttctt catatatttt gcattatctt tctgaatatc atgatgtgat ctaataggct 1080

gttttaaatc atcattaact acctgaccag gtccttgtga gcaaggtcct ccaacaaata 1140

gcataactcg ggcgccagta ttggaatatg tacattctaa caaaccaatg gcaatcgata 1200

aagctacacc tgtagatctt aaataccttt taccctgatg aacaggccaa ggatctttct 1260

gttgttctcc tattaggtct gttagattca tatcgcattt ggatacaggt tgtagaaacc 1320

tgcttcctgg tgcaacaaca gtaggttgca tttgcccagg tcttagaggc tgccctggct 1380

gttgaggggc ttgctgacct aaagccactt tgcctattcc cagcatttct tgaacctgtt 1440

tagcactaag atcttttgta cctctgaaca catatgactt actacaacct tcagtgccaa 1500

gttcatgaac ttgaaccatt ttcccaaatg ttattagtcc tattaacgca ttaggtggca 1560

acaaactaag ggacatttgc aatgagtctt tcagggaacc cagttcttca tcatccatgc 1620

aggtgtcaac aacatacaaa aatattggtg gtaaacattg agctctagtt attgtgtatt 1680

caatggtgga aaacataggc atcaattccg ctggttgatg ttgttctgaa atagcagcat 1740

attgaggtgg aaatggattt ctctggaaac aaaagttgca tacccagagt tttgctcgat 1800

aatctacctg acacagtggg tttaatattg ctctacaagt atttctagta cataaaacag 1860

ggtcatattg tattggtgga agatccagac gttcctttat aggctggtac agacaagcta 1920

agggtactac gagacgggta gcttcaattc tgcttgaagg ccatacattc caggtaaatc 1980

taatcccatc tcgatcttca ttttgttgta tatactcttc atatgtgctc atttttgaag 2040

attacaagtc ggaattccct aaggaaattc ctcaatatat attattagga ttctatcatg 2100

taaataacac tattattgag ctcctaaaaa ttacacctaa ttgtttcaga atttgaac 2158

<210>392

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>392

ccgacttgta atcttcaaaa atgagcac 28

<210>393

<211>33

<212>DNA

<213>Diabrotica virgifera

<400>393

gctaacgcat cataatgttt aatacctttc ttc 33

<210>394

<211>1054

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(234)

＜223〉n=a, c, g or t/u

<400>394

ccgacttgta atcttcaaaa atgagcacat atgaagagta tatacaacaa aatgaagatc 60

gagatgggat tagatttacc tggaatgtat ggccttcaag cagaattgaa gctacccgtc 120

tcgtagtacc cttagcttgt ctgtaccagc ctataaagga acgtctggat cttccaccaa 180

tacaatatga ccctgtttta tgtactagaa atacttgtag agcaatatta aacnccactg 240

tgtcaggtag attatcgagc aaaactctgg gtatgcaact tttgtttcca gagaaatcca 300

tttccacctc aatatgctgc tatttcagaa caacatcaac cagcggaatt gatgcctatg 360

ttttccacca ttgaatacac aataactaga gctcaatgtt taccaccaat atttttgtat 420

gttgttgaca cctgcatgga tgatgaagaa ctgggttccc tgaaagactc attgcaaatg 480

tcccttagtt tgttgccacc taatgcgtta ataggactaa tacatttggg aaaatggttc 540

aagttcatga atttggcact gaaggttgta gtaagtcata tgtgttcaga ggtacaaaag 600

atcttagtgc taaacaggtt caagaaatgc tgggaatagg caaagtggct ttaggtcagc 660

aagcccctca acagccaggg cagcctctaa gacctgggca aatgcaacct actgttgttg 720

caccaggaag caggtttcta caacctgtat ccaaatgcga tatgaatcta acagacctaa 780

taggagaaca acagaaagat ccttggcctg ttcatcaggg taaaaggtat ttaagatcta 840

caggtgtagc tttatcgatt gccattggtt tgttagaatg tacatattcc aatactggcg 900

cccgagttat gctatttgtt ggaggacctt gctcacaagg acctggtcag gtagttaatg 960

atgatttaaa acagcctatt agatcacatc atgatattca gaaagataat gcaaaatata 1020

tgaagaaagg tattaaacat tatgatgcgt tagc 1054

<210>395

<211>910

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(833)..(893)

＜223〉n=a, c, g or t/u

<400>395

ggagagtaca cgtcaacgtc atctcagttt ggtttattgt tgggtgtaat gtttaaataa 60

aataattatt attttgtaaa cctgtgccat taaacaaata aagcgatggt gctaattgca 120

gcagcagtct gcacgaaagc aggcaaaaca attgtgtctc gacaatttgt tgaaatgacc 180

aaagctagaa tagaaggttt gttggctgcc tttcctaaat taattcctac aggaacccag 240

catacatttg tggaaacaga ttcagtacgg tatgtttatc aacccctaga gaaactgtat 300

atggttctta ttacaactag agctagcaac atattagaag atcttgaaac cctccgtcta 360

ttcgcaagag tgattcctga gtactgcaaa tctttggatg aaaatgaaat tgcagagaat 420

gcattttcac ttatatttgc ttttgatgaa atagtggcat taggttatag ggaaagtgtc 480

aatctgtctc aaattcgcac atttgtggaa atggactcgc acgaagaaaa agtttatcag 540

gccgtgagac agactcaaga gcgcgaggcc aagaatatga tgagagaaaa ggcaaaagaa 600

cttcagagac agaagatcga agcggccaaa aaaggaggga agacctcgtt tggtagtagc 660

ggtggctttg gcagctcgac aggttatact cctacgccat ctgttggtga tgtagctaac 720

cagacaaatg atgttaaaac ttcttcatac acaccagccc ctgcgcaaaa acctcggggt 780

atgaaattag gtggaaaagg tagagatgta gaatcattcg tagatcagct tanatcggaa 840

ggagaaaacg tcattactcc aaaacaaaat agtatttcac agccaggaac tanagctcca 900

gctatcaaaa 910

<210>396

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>396

gagtacacgt caacgtcatc tcagtttgg 29

<210>397

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>397

gctgatctac gaatgattct acatctctac ct 32

<210>398

<211>826

<212>DNA

<213>Diabrotica virgifera

<400>398

gagtacacgt caacgtcatc tcagtttggt ttattgttgg gtgtaatgtt taaataaaat 60

aattattatt ttgtaaacct gtgccattaa acaaataaag cgatggtgct aattgcagca 120

gcagtctgca cgaaagcagg caaaacaatt gtgtctcgac aatttgttga aatgaccaaa 180

gctagaatag aaggtttgtt ggctgccttt cctaaattaa ttcctacagg aacccagcat 240

acatttgtgg aaacagattc agtacggtat gtttatcaac ccctagagaa actgtatatg 300

gttcttatta caactagagc tagcaacata ttagaagatc ttgaaaccct ccgtctattc 360

gcaagagtga ttcctgagta ctgcaaatct ttggatgaaa atgaaattgc agagaatgca 420

ttttcactta tatttgcttt tgatgaaata gtggcattag gttataggga aagtgtcaat 480

ctgtctcaaa ttcgcacatt tgtggaaatg gactcgcacg aagaaaaagt ttatcaggcc 540

gtgagacaga ctcaagagcg cgaggccaag aatatgatga gagaaaaggc aaaagaactt 600

cagagacaga agatcgaagc ggccaaaaaa ggagggaaga cctcgtttgg tagtagcggt 660

ggctttggca gctcgacagg ttatactcct acgccatctg ttggtgatgt agctaaccag 720

acaaatgatg ttaaaacttc ttcatacaca ccagcccctg cgcaaaaacc tcggggtatg 780

aaattaggtg gaaaaggtag agatgtagaa tcattcgtag atcagc 826

<210>399

<211>1057

<212>DNA

<213>Diabrotica virgifera

<400>399

aatagaagca actgcttaaa aatcacacat attccttcgg tgttttagtg tttgttctta 60

aacaatatta ataaaaagtt ttagtttttt tcaatcatgt cagcagggga tgcactagcc 120

cccggtgcgg gggcggcggg tagcggcggc gacgatggca ttgtgggcgg tccgcgaacg 180

ccacaacaaa tcgcggccca acgacggctt caacaaacac aggcgcaagt cgacgaagtc 240

gtggatatca tgaaaacgaa tgtagaaaaa gtattggaaa gagatcagaa actatcagaa 300

ttagatgata gagcagatgc tttacaacaa ggtgcttcac aatttgaaca gcaagcggga 360

aaactaaaga gaaaattttg gttacagaat ttaaagatga tgataataat gggtgttatt 420

ggtcttgtaa tcttaataat catagtcatg aacttcatgt aactcatggg ccaaaaattc 480

gttcgaataa ttggcatata cagaaaacct acaattattg aatacaacac accaatacaa 540

aaaatcaata ttaaagtaat ttaatctata ggttggattg aaatagacga ttaggcaagt 600

tggttgaaaa aaatataatc agttgttaaa ggttcaataa atggacactg gtgtttcacc 660

aagtaattgt aattcaataa taataagcct ctggaaacac cattatttta ttatcacggt 720

aaaatacgag tgtctagctg tcaaaaatgt taaaattgtg tatttaacga aaaaaattaa 780

tataattagg tgaacagaac gaaaattttg tttttttaat attattgtag taagcgaaca 840

ctttgttatt ttttttaagg tttaattaaa agaaaaaaat aaagttttaa ctttttttaa 900

ttttataaga caattaaaga agatttaatt taattggttg gttaaaaaaa agtattcttt 960

ttacaatcct ggaaaaaatc tttgtaagtt tcgataaaaa aggcttgttc aaatccaccg 1020

ccctcccccc caacccgaat atttggaaaa ttttttt 1057

<210>400

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>400

caatcatgtc agcaggggat gca 23

<210>401

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>401

ccaacttgcc taatcgtcta tttcaatcc 29

<210>402

<211>511

<212>DNA

<213>Diabrotica virgifera

<400>402

caatcatgtc agcaggggat gcactagccc ccggtgcggg ggcggcgggt agcggcggcg 60

acgatggcat tgtgggcggt ccgcgaacgc cacaacaaat cgcggcccaa cgacggcttc 120

aacaaacaca ggcgcaagtc gacgaagtcg tggatatcat gaaaacgaat gtagaaaaag 180

tattggaaag agatcagaaa ctatcagaat tagatgatag agcagatgct ttacaacaag 240

gtgcttcaca atttgaacag caagcgggaa aactaaagag aaaattttgg ttacagaatt 300

taaagatgat gataataatg ggtgttattg gtcttgtaat cttaataatc atagtcatga 360

acttcatgta actcatgggc caaaaattcg ttcgaataat tggcatatac agaaaatcta 420

caattattga atacaacaca ccaatacaaa aaatcaatat taaagtaatt taatctatag 480

gttggattga aataacgatt aggcaagttg g 511

<210>403

<211>882

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(818)

＜223〉n=a, c, g or t/u

<400>403

gttcataaca gttaatcatt tattgtttga cttaacaaaa agtagaccta gagtgaccac 60

cattaaatgt aatggtcagt taacaaatgt agtgttcaca aattatgaaa aaaacctatt 120

actccttatg ttgccagaca acagagtctc aaaacaagaa atattgttgt taaataatga 180

aataattaga ttattaaaat tctgctatga aactattgac aaatgcttca taactgaaaa 240

atacctccca caagtagacc acttcttttc gaaatttttt gcaagaactc ttagtagtgg 300

tttgtggagc acagctcaac aatctctggc tttggaaaac ctagaatcaa aagcaaaaga 360

agtcagcccc tgtcagtttg aggacttgat gcctgttgca cccactttgt gtctgcctga 420

cgaggctcag cttcagatag atgatgcctt atcagaattg gaggcaagtg attatagaga 480

ctggaatgaa gacccattgg actgtcaaag actgttcaca gttctgggca gtgccctctt 540

tcattcagga tacctgttgg cttcacattt tattcacgaa gatttgatag atgtcttttg 600

tttctgtaaa caacaaggat tgtttcattt atcaaggaca gaacctataa aatcacttgt 660

tctttggaag gaggtatttc catcatcttg caacaaattg gaaaatacaa aaattgttat 720

tcctgaagga agaaggtatt tgctaatagt ggaaagtgga aaagatctgt tagcggtaat 780

aatggaagct gggggttgta cagaaccatc tgaagaanac atgggtcctg atgcattcta 840

cgtagaagaa gcgcaagcta ctttagccca catccaagaa tt 882

<210>404

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>404

cctattactc cttatgttgc cagacaacag ag 32

<210>405

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>405

cttggatgtg ggctaaagta gcttgc 26

<210>406

<211>763

<212>DNA

<213>Diabrotica virgifera

<400>406

cctattactc cttatgttgc cagacaacag agtctcaaaa caagaaatat tgttgttaaa 60

taatgaaata attagattat taaaattctg ctatgaaact attgacaaat gcttcataac 120

tgaaaaatac ctcccacaag tagaccactt cttttcgaaa ttttttgcaa gaactcttag 180

tagtggtttg tggagcacag ctcaacaatc tctggctttg gaaaacctag aatcaaaagc 240

aaaagaagtc agcccctgtc agtttgagga cttgatgcct gttgcaccca ctttgtgtct 300

gcctgacgag gctcagcttc agatagatga tgccttatca gaattggagg caagtgatta 360

tagagactgg aatgaagacc cattggactg tcaaagactg ttcacagttc tgggcagtgc 420

cctctttcat tcaggatacc tgttggcttc acattttatt cacgaagatt tgatagatgt 480

cttttgtttc tgtaaacaac aaggattgtt tcatttatca aggacagaac ctataaaatc 540

acttgttctt tggaaggagg tatttccatc atcttgcaac aaattggaaa atacaaaaat 600

tgttattcct gaaggaagaa ggtatttgct aatagtggaa agtggaaaag atctgttagc 660

ggtaataatg gaagctgggg gttgtacaga accatctgaa gaagacatgg gtcctgatgc 720

attctacgta gaagaagcgc aagctattta gcccacatcc aag 763

<210>407

<211>3045

<212>DNA

<213>Diabrotica virgifera

<400>407

ggttaagtac ttatcggacc gcctgcctac tagtttctgt tgtggttgat agtttcactt 60

taatttcttt atctctgcgg cgtaagatgc gcccaaaaca ttaagaattt aagtgttgct 120

ttaatgtttg agtttctcgg ttaaatattc acaatttttt ttctcttttc aacaaaatta 180

aatctgaaag tacacaatga tcactgccaa aaaatataga ataactaaga agcaaatttc 240

gcgatatgag accgttcctg taagaaaaat gctagatttc gtatatcttg gtctatgaat 300

aacattaaca taagtattta ataatagata tttcataaaa ataaacatcc cctgctagat 360

ttagagactt ctcgcgaaat tgcgaaagag aactaaaacc gaatcaaatg caacactggc 420

ggttaaattt atactcttaa cgaagttaaa gaacaatcag caactataat ttataatcta 480

atgcttactc taatctatat aattttgaat atgattgtta agttgttgat tttcattgtt 540

ggttgctgtt ttcgccgttg acttgatttg gccgagcttt cgacgcatta tgtggagtca 600

tcagtacttt cagtacttca atttaaaaat ttttatcttt tgttcttctt atttttcatc 660

taaatacaaa tatatggtaa aagtaagtta tatagatata aagcggtatt gtttgagtaa 720

aaacggcaac tgtaggtaaa aatctgttaa tgattttgaa attgtaattg cattagccgc 780

cagatgtcgc catcggtgac agttctcccg actttcgacg aaaggtagga gcgtatcgag 840

cgccgtcaaa agttgaggtt atgtttaaaa tactaacctt agttgttaag ttagttgcga 900

accgttgcaa ctctcttccc tcactgtttg ctgcattaaa atagataaaa tggcctggcg 960

acgtacctgg actcgcgaat aaaggttcac cgacattgaa actgaataaa aagagagaaa 1020

actttgctgg tttctttttt ctacgatatt aatcgatgtc ttccgaaacg gaggcattgc 1080

ggcctattac cggcactaaa taagtagaga tgttatgatg tttataagtt tttatgtttt 1140

ttaatttttt tgctatgtat gttgtcgctc cgagctataa tacggttagg tacgtactgt 1200

aagcctattt tgggcaacag cctgtggaca taccaaacaa aagattgctc atgtactata 1260

tctctatcac tgatatgtat ttaattcgaa tttatcgtga gtaatctcta aagaacaata 1320

ctacgacaat aaacgatgga gtgattctat taaacaaaac aatactttca ggatgatcca 1380

cgggctggcc tacatatcgg gacatacact ttagatggca aaaattagaa taaatgggcg 1440

gggggtaaga aattctgtga tattaaaaaa tgatttcatg tctacattga aagtgatacg 1500

aaggaaacgt tagtaaagac gacgaaatcg actaatgctt ttggaagtat actgcgggac 1560

actcacaatg cggactagat ttaacttcga aacacgtcgc attccgtcaa gtcacatgat 1620

ctactttgtc cgtccccgat tttgaaataa cacgaaacaa cagtgaccga ttttcattag 1680

tgtgtttggc gacgtgaccc tctagtgatt gaaatataaa ctacgttgtg aaaagtgaca 1740

atgacagtta catcacttgt caaaagtgac tgacaatgga catttatgtc acttgtcaga 1800

agtaattgac aattcaacaa aattcaggcg aaagtttaca gtaatattac tttatagtcc 1860

aaataattaa aatatttttg cgtttgcttg atttgagtct gctcggtatg gtaaacaaag 1920

gcattagaag ctgttacaac aataatgggc aacatttttc ttttattata actgtctata 1980

aaaatgttta ttatttactt gttgtggaac tgtagtttcc tggaatcagt cggagtatga 2040

tattatattt tgaaaggttg gcaacgttat ggaaacgtat ccaacatcga tgttgccaaa 2100

tttccaatgt gatctgggat tgccgcgcaa tctgcacatt cttgtcgagt ttttgtacta 2160

aggggaaacg aaaaatggaa aacgaaaaaa acgatctaaa gtgtctggac cgtatctcca 2220

tatatgcata cttgtcgcat atcattgtgt tatcaatata ttttatattt atattttttg 2280

aacttttata tatttttatt tgtgtcataa cttctctgcc cccttttttc gaaaaaaaac 2340

aggcagtgtg atcgtgcctt ccttacaatt tcatttttta tacgatcgta ctaaaattgg 2400

tgctactata gtatgcggtc tacctgtgca tgtgtataga gcctacaaac agttttctgc 2460

ctttcattat tgtatacaaa cccttctgag aagagattat ggtgttgttc tgtagaccgc 2520

aagctatagc agaaacgcga cggtagaccg cataccatag tggtacccta aaattatctc 2580

gcccgtacca cctcgatgcg gaaaaggaat ctcggttgtc ttggcctctc gtccgttcct 2640

gtgtccagat tatattttca tacattggga aagcgggtct aggatggctc ctcggggtac 2700

acagattgtt aacggaagtt tttggaacgg aggcgaggcg aacggtacta ccgcagcttc 2760

ttttttcctt tgttactgga tcatctgtcc tgagaggtaa cagaatttgt attttataat 2820

tttttttatt taattatatt ttaggtatgt taaaactgaa cgtatatgat gaaaataatg 2880

actgattgtg tattgttgaa tccgattcgg cagtacttaa tacatttgtt tgtaaaattt 2940

tgagttttgc tgttaatcat tgtatatatg ataaatatat tattttacag agtccttgag 3000

gtcaattttg ttagcccagg actaacctga gggagagaat atatt 3045

<210>408

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>408

tgagtctgct cggtatggta aacaaagg 28

<210>409

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>409

gctaacaaaa ttgacctcaa ggactctgt 29

<210>410

<211>1125

<212>DNA

<213>Diabrotica virgifera

<400>410

tgagtctgct cggtatggta aacaaaggca ttagaagctg ttacaacaat aatgggcaac 60

atttttcttt tattataact gtctataaaa atgtttatta tttacttgtt gtggaactgt 120

agtttcctgg aatcagtcgg agtatgatat tatattttga aaggttggca acgttatgga 180

aacgtatcca acatcgatgt tgccaaattt ccaatgtgat ctgggattgc cgcgcaatct 240

gcacattctt gtcgagtttt tgtactaagg ggaaacgaaa aatgaaaacg aaaaaaacga 300

tctaaagtgt ctggaccgtg tctccatata tgcatacttg tcgcatatca ttgtgttatc 360

aatatatttt atatttatat tttttgaact tttatatatt tttatttgtg tcataacttc 420

tctgcccctt tttttcgaaa aaaaaacaag gcagtgtgat cgtgccttcc ttacaaattt 480

cattttttta tacgatcgta ctaaaattgg tgctactata gtatgcggtc tacctgtgca 540

tgtgtataga gcctacaaac agttttctgc ctttcattat tgtatacaaa cccttctgag 600

aagagattat ggtgttgttc tgtagaccgc aagctatagc agaaacgcga cggtagaccg 660

cataccatag tggtacccta aaattatctc gcccgtacca cctcgatgcg gaaaaggaat 720

ctcggttgtc ttggcctctc gtccgttcct gtgtccagat tatattttca tacattggga 780

aagcgggtct aggatggctc ctcggggtac acagattgtt aacggaagtt tttggaacgg 840

aggcgaggcg aacggtacta ccgcaggttc ttttttcctt tgttactgga tcatctgtcc 900

tgagaggtaa cagaatttgt attttataat tttttttatt taattatatt ttaggtatgt 960

taaaactgaa cgtatatgat gaaaataatg actgattgtg tattgttgaa tccgattcgg 1020

cagtacttaa tacatttgtt tgtaaaattt tgagttttgc tgttaatcat tgtatatatg 1080

ataaatatat tattttacag agtccttgag gtcaattttg ttagc 1125

<210>411

<211>1844

<212>DNA

<213>Diabrotica virgifera

<400>411

gggcggacgc gtgggtttgt ctgtgcgaac ttctaacctc atttttacag ccttaacata 60

cgtttgacgt ctgttaatgc tttttaacta atagagattg tctaaaatgg cagtaatcgt 120

ttacttttgt ttcttccggg atgctcaaaa accatcaaca cagtttgtat aggaactaag 180

atttttgggg agactgaagg agacgttgac aggggttctc atacctgcct agagaagata 240

atacaataat acattttata tatttattaa taatatagta ataatgttgc gcgtgcgttg 300

tctactaagc attgtggatt tggtcgatct tccgatttag tttaagaaat aaaaatatat 360

gggcaacttt tttttggttt tgtttcctgg ccttggtgtg aaaccttagc cacgtaatta 420

ctataactat ctcgggggag taatgtgtag tgtgtgttga gtaaatgggt tgttacttag 480

taaattcggc ttcgtaaaga caaagtttct acccagactg gattcgaact tattgggttc 540

aaaggtaggc tctcttacca ctgagccacg gaagcttgta cccgtctttg ttaagattag 600

tttatagttg gcgaagaaca tttcagtaag tttagctcgc gtatttcgcc tctaatcttg 660

gaaaaaatat atggacgtgt ttcaaatttc aaacaaattg tttattttaa gaaaaacgtt 720

acgaaaacat atttagttca aaagtactaa ttcctcaata tatttcatta tttgaactaa 780

catttttttt atcctgatta ttgatttaga gatgtgtaca aatcaacaat gcattagtga 840

atccttcact ctctcttagt gtacataatt tgaaacaatt aattttacat ccaattatag 900

gtccatgaca tgtgacacat tcatgtcata tatcattaag tagcaattgc tcaattctat 960

gctttcgaaa ttataaagta aatcctttct gcaccagtaa aacagaattg aacaattgtg 1020

tcagcagaaa catatatttt tggatagtaa tgggagtcga atgagtgtcc ttagatagga 1080

ttttgttatg attaaagtaa actttattgt aacttactga gcatggggat acgtgagcgt 1140

ggggatatac tcggacattt actcaaataa tccttatttt tgaagcttgg ttaggcaaat 1200

acagagacaa cttaaaaaca ggttaatttc aaattttctt tgccacactc ggtgaaatag 1260

tcggacaagt ttgctagtaa aatactaaga caacttaaaa acacagatat gttaaaaaaa 1320

atcataatgc agatgtgggg ctaaactaag acatgtggga atgtccgggt atttcaccac 1380

aactatgatt agaaaaggca aatattaatc ggataataaa aaggtatttt cagatatctt 1440

ccttttaacc caggtatttc caacaaatca attagtgcaa tattaggata ttgctcataa 1500

acaccacaaa gcagccgatt gaacaaatct tagtatctct ccagatagct tatgaaattt 1560

tacatcagat agagcagata actcaaaagt caaatatttg aagatatcct agtattgttc 1620

tagtgcgacg atatatatac caaaaaatta taatttaaaa ataaaaatcg acctgtttcg 1680

ggatttttct ccagagtcgc ccattctcga gaaaataaat gtattccaac tcaaacgttc 1740

tcactgtata aaaaatgaga ctaaaagaca taaaaaatag acagatctga tgttttcaaa 1800

atctctaata tttcgtacag ttgttcacat aaaacatctt tttg 1844

<210>412

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>412

ggagactgaa ggagacgttg acaggg 26

<210>413

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>413

ctcacgtatc cccatgctca gtaagttac 29

<210>414

<211>948

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(38)

＜223〉n=a, c, g or t/u

<400>414

ggagactgaa ggagacgttg acagggtttc tcatactngc ttagagaaga taatacaata 60

atacttttta tatatttatt aataatgtag taataatgtt gcgcgtgcgt tgtctactaa 120

gcattgtgga tttggtcgat cttccgattt agtttaagaa ataaaaatat atgggcaact 180

ttttttggtt ttgtttcctg gccttggtgt gaaaccttag ccacgtaatt actataacta 240

tctcggggga gtaatgtgta gtgtgtgttg agtaaatggg ttgttactta gtaaattcgg 300

cttcgtaaag acaaagtttc tacccagact ggattcgaac ttattgggtt caaaggtagg 360

ctctcttacc actgagccac ggaagcttgt acccgtcttt gttaagatta gtttatagtt 420

ggcgaagaac atttcagtaa gtttagctcg cgtatttcgc ctttaatctt ggaaaaaata 480

tatggacgtg tttcaaattt caaacaaatt gtttatttta agaaaaacgt tacgaaaaca 540

tatttagttc aaaagtacta attcctcaat atatttcatt atttgaacta acattttttt 600

tatcctgatt attgatttag agatgtgtac aaatcaacaa tgcattagtg aatccttcac 660

tctctcttag tgtacataat ttgaaacaat taattttaca tccaattata ggtccatgac 720

atgtgacaca ttcatgtcat atatcattaa gtagcaattg ctcaatttta tgctttggaa 780

attataaagt aaatcctttc tgcaccagta aaacagaatt gaacaattgt gtcagcagaa 840

acatatattt ttggatagta atgggagtcg aatgagtgtc cttagatagg attttgttat 900

gattaaagta aactttattg taacttactg agcatgggga tacgtgag 948

<210>415

<211>1700

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(49)

＜223〉n=a, c, g or t/u

<400>415

attaagtatt aattactgat atggtatgtc cgctaaatga tccagcatnt gatgctggaa 60

agataaaata atctagtaat tacaataatt gttttacaaa catttaaatg cataaataaa 120

agtaatttag aatggatatt attgctacaa ttttattgtg tcaataatag ggtacaatac 180

gtttacttta gcggggaagg ttagtttaga gataattcaa aaatttggta gtaagatatt 240

ggatttctta taattagatc ttttaaagaa taaattatat acagacgtgg atttaattga 300

gtattatggt ttaagtagct ttatatatac aatatccata atgaatgatc attttatgat 360

tgtccagcta ttcaagcatt ttttttatca atataacaac ttgaaggtgt tataatattg 420

gaatctatcg ttttcgttct catttggagt acccatctca aaatatgatc tctctagtta 480

tttaattata ttaaataaag gtgagaaaac gtgaaccttt acaaaatggg gaggggtggg 540

aagtagaaaa tgtatgaata tgttcttcct ctgtactagt cggtagagga cttttgaaat 600

tactctttta tatatatgaa tcgtcgtcag tttttctcta tacgttagag tttccgttcc 660

accaattctt cggacgggta gatagacgaa tgcattccac aaaactaaag gattgatttt 720

tctattttga tttcgatcca attgaatagt taagtcctat taattagata ttgaccttct 780

tttgtctgat ttgcttaaag taatttatta ttttctttct ttcgcttgac actgtacatc 840

tattcatctt cttaatatcc gatattcttt gaaaacacat cttaggtcgg cagtacacgg 900

acagcttgaa ccagcgaccg cacggtgaac tataatccta tattcgctga tatacacatg 960

acagctcgag cacagtcacg acctcttcaa actgtcaaca gcacgatgaa aatccatcgt 1020

gctgttgaca gctcgagagc ggttgcaagg caacaagtga caactcaagt agtcaacgac 1080

tgctccagat atcaacgacg accgctcgag cagtccgtgt atgtcgctca gtggtaaact 1140

gcttcaacag tccttgtacg gccgccctta gagctatatt atattcttaa aatctacagt 1200

taaagctgat tttgattccg ttttataatt cctccaaatc aagagggtag gtcaaagcag 1260

agtgagttgt gttgattttt ccttatgtgt gtgacgattt cacctggagg tatttccctt 1320

tgtaggaaga tctttgaaga tctcttaatg gaaaattatt ttctgtaata tattattcat 1380

actccattta tgtaataaca acattatagt acactaaagt atctaaaatg tttttatatt 1440

aacaggtatg tgaaaaagta acaaaatgag aattattaaa aagactagta gtacttgaag 1500

ctgatgctta caaaggccct caatatcaac ttacccattt tagaaattgg aattatttga 1560

gtaatactaa aactttgagg agttacttag gttctggaga ctttgattga agatcctctt 1620

gagattttaa tactacaaag atatttaaca ctgcgttaaa atttttgcta ttaaatattc 1680

agtatactct aatttcgaga 1700

<210>416

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>416

gcagtacacg gacagcttga accag 25

<210>417

<211>40

<212>DNA

<213>Diabrotica virgifera

<400>417

gcagtgttaa atatctttgt agtattaaaa tctcaagagg 40

<210>418

<211>764

<212>DNA

<213>Diabrotica virgifera

<400>418

cagtacacgg acagcttgaa ccagcgaccg cacggtgaac tataatccta tattcgctga 60

tatacacatg acagctcgag cacagtcacg acctcttcaa actgtcaaca gcacgatgaa 120

aatccatcgt gctgttgaca gctcgagagc ggttgcaagg caacaagtga caactcaagt 180

agtcaacgac tgctccagat atcaacgacg accgctcgag cagtccgtgt atgtcgctca 240

gtggtaaact gcttcaacag tccttgtacg gccgccctta gagctatatt atattcttaa 300

aatctacagt taaagctgat tttgattccg ttttataatt cctccaaatc aagagggtag 360

gtcaaagcag agtgagttgt gttgattttt ccttatgtgt gtgacgattt cacctggagg 420

tatttccctt tgtaggaaga tctttgaaga tctcttaatg gaaaattatt ttctgtaata 480

tattattcat actccattta tgtaataaca acattatagt acactaaagt atctaaaatg 540

tttttatatt aacaggtatg tgaaaaagta acaaaatgag aattattaaa aagactagta 600

gtacttgaag ctgatgctta caaaggccct caatatcaac ttacccattt tagaaattgg 660

aattatttga gtaatactaa aactttgagg agttacttag gttctggaga ctttgattga 720

agatcctctt gagattttaa tactacaaag atatttaaca ctgc 764

<210>419

<211>1638

<212>DNA

<213>Diabrotica virgifera

<400>419

gaatatcaac aaaatgccaa gggaaaaaac aaaaaatgat taataaaact aaaatctttt 60

ttgtttttcc cttttctcaa aactagaaca tacaacagta taactctggg ctaacaaaat 120

gccaaggtac agaagtattc aacagcatga ggtcactttt gaatgtggtt tatctaatgt 180

tgccgcaaca cacttcactg tgtgttattt gttatgcacc ttattcagcc acataggact 240

aagtgtggag ggttcattca tcaaaattcg ctttatgtgc acacatagca cataagcata 300

catggcaaat atgcctatcc atccagttgt cagtttttac acacttcaaa agacaaatat 360

ttgggtgcat acagagtagc cacgtaacaa atagcacata gcgaagtgtt gtaccagcat 420

aatgtataag cttatacaaa actaggttta cctgtatctg ttacagtaac gctgtaactt 480

gcatacacat cagtatatcg agttgtctac aagtacattg tatttttttt tataaagacc 540

tgtcataata ttcatttaat ctgtctggca gatgtttgta tgtttactga gtcaatgatt 600

tggtgatatt ttcaagtaaa tttacgggaa aattaactat ttaataaaaa cagatcacat 660

agataactgt gtggttaggg gacacaagca gacaagtcaa cttttcagtt ttatagttaa 720

aattaatttg acgggtccac tgaataatgt aagtgcctaa tcacttgttt ttaagaaata 780

gaaaaaaagt gttaaaatta taacaatggc aaacttatac atttaaaggt tgtactcacc 840

cttaaaatgt ataaactcac cattatcaca attttaaaac tttttcttca acttctcaaa 900

aacgaacaat gggatactta caatgttatt cggcggaccc attcaattaa agctaaaatc 960

gtgagtgtga aaaagatcac tatttcatat atgaaactag acatttaaca ttttgaactg 1020

tcatctattt cagcagtaga ttattgtttt aggtgttttt aaccctgcag cggacaggcg 1080

aaataaaatt acattgtatt tccatatggc tgctgtccac tggaggtttc ataaataaaa 1140

atgtttctca aaaattgagt tgtctgccac cacacaatta tgagttatat aaccactgca 1200

ttatgtttta cctgtaatta acatgtctaa tgttgataat tttaacaaaa cagcataatc 1260

aatttgtatt tttaaatgta ttattaaata ttttgtattt tgatactaaa tattttgtat 1320

tttgataata aattagatat taaagataaa gatttatata ccctattcca cgaacatacg 1380

actgttgtgg attatctcga caacgaatat tttactgtgt aaattataaa gaatgaaagt 1440

aaattgcaaa atacattgtt gtttattgga acaattagta gagccattta ctttcgcact 1500

tcttatgttg cacactaaaa tattcgttgt ggcgataatc caagatagtc gtatattcgt 1560

ggaatagccc atactaaaat tataaaaaaa atgcactaga aataaacaac ccaagacacg 1620

ttgaatgtta caaggagc 1638

<210>420

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>420

ggtacagaag tat tcaacag catgaggtca c 31

<210>421

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>421

gaaacctcca gtggacagca gcc 23

<210>422

<211>1005

<212>DNA

<213>Diabrotica virgifera

<400>422

ggtacagaag tattcaacag catgaggtca cttttgaatg tggtttatct aatgttgccg 60

caacacactt cactgtgtgt tatttgttat gcaccttatt cagccacata ggactaagtg 120

tggagggttc attcatcaaa attcgcttta tgtgcacaca tagcacataa gcatacatgg 180

caaatatgcc tatccatcca gttgtcagtt tttacacact tcaaaagaca aatatttggg 240

tgcatacaga gtagccacgt aacaaatagc acatagcgaa gtgttgtacc agcataatgt 300

ataagcttat acaaaactag gtttacctgt atctgttaca gtaacgctgt aacttgcata 360

cacatcagta tatcgagttg tctacaagta cattgtattt ttttttataa agacctgtca 420

taatattcat ttaatctgtc tggcagatgt ttgtatgttt actgagtcaa tgatttggtg 480

atattttcaa gtaaatttac gggaaaatta actatttaat aaaaacagat cacatagata 540

actgtgtggt taggggacac aagcagacaa gtcaactttt cagttttata gttaaaatta 600

atttgacggg tccactgaat aatgtaagtg cctaatcact tgtttttaag aaatagaaaa 660

aaagtgttaa aattataaca atggcaaact tatacattta aaggttgtac tcacccttaa 720

aatgtataaa ctcaccatta tcacaatttt aaaacttttt cttcaacttc tcaaaaacga 780

acaatgggat acttacaatg ttattcggcg gacccattca attaaagcta aaatcgtgag 840

tgtgaaaaag atcactattt catatatgaa actagacatt taacattttg aactgtcatc 900

tatttcagca gtagattatt gttttaggtg tttttaaccc tgcagcggac aggcgaaata 960

aaattacatt gtatttccat atggctgctg tccactggag gtttc 1005

<210>423

<211>1602

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(1543)

＜223〉n=a, c, g or t/u

<400>423

ctaatttagg ggcttagatt agtatttcct tatacaataa gttaaaaaat atttattttc 60

ttttagttta attgtttcgt ctgtagcact ataagattat taaataattt gtgttaatta 120

ctacttaaat tacaaagttt attgttactc tagactctag acctaacaaa ttatatgtta 180

tcttaaaaaa atagtgtgtt cactgcatga tactccacaa ataagaatag ttcagacagt 240

aactttcttt taactatcga cacataagat aacatttact ccttggcaag ttcattggtg 300

ccctaatcaa tcacaatagg gaacttgcac ttttttgttt tattatataa tattgtttag 360

tttcctttaa aaataagatt tccaaaattt cacgcctcaa aatctcataa taacttagaa 420

gtacaaattt aaaatcttct atgtatttaa atcatttcaa acgatctaaa aatcgcgcga 480

attcttgtga cgtcatatga tcatattttg tatgtcattt ctaacgtctc atttaaatta 540

catacaattt tgtttacttt ggagttcaaa aacacagttt ttgaagagat agtaatgaat 600

tgtgtgtatt tttattatgg aaaataaaag taaatactat gaaagtgttg tttaataaca 660

ttctgttaaa ttaaaaccat tgaaactccc aataaaatat ttattagatt accagcggat 720

aagaaacgga gattaataaa gtggttacaa aggacattaa agacatgtta gttcattctc 780

aaggtcttca tggatgcgaa gctaatgcta atataggtgt ctgttgtttt aatgtaatat 840

gtgttgtctg cttaatagtc tttttttata aatcaagtat atatagatgg aaaagataaa 900

aataatatat aatatcaatt gtgtgaaaaa ttttcttcct tttgaaaata tgtggcacct 960

ggacaacata aatatattta caatgggtaa agttatcaga ctgcccttta ggaaaaccct 1020

cttcggtcat tttaaaaaat tgtcttaaca aaaacactca aatattatga aatatatagt 1080

tcacagcaac tatctaaaga tttataaact gaaatagacc ataaaatgat tacaataatc 1140

ataaacaata ttaaacaaaa acttaatgaa ttaacgacgc tgaatttaaa cattataata 1200

atacgacgtc gcgaccaatc aggtcgcctt ttgggctggc cgctataatg tgaattgtct 1260

ctcgatatta atcatcttta ggggccaaat gaaagaaaaa aacaactttt ttcctttggt 1320

atatatttta aattatggtc tgttgggatt tatagcattt tgtcgaattt aaaattctat 1380

gtaagttccc tattcattta ggattgatga gagattgtaa agatcaatta tgtcaacaaa 1440

gctaaaatca ttgtgaaaaa gtggttattt acatagctac ataaatagct ttttcttaca 1500

tttgtcaaag aatagagttg aaagggacac tacatagtct tanaactaat tatttcaaga 1560

agtattattc attgtcttga gtatttcaaa ctgaaacatc gt 1602

<210>424

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>424

ctccttggca agttcattgg tgc 23

<210>425

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>425

ctccgtttct tatccgctgg taatct 26

<210>426

<211>453

<212>DNA

<213>Diabrotica virgifera

<400>426

ctccttggca agttcattgg tgccctaatc aatcacaata gggaacttgc acttttttgt 60

tttattatat aatattgttt agtttccttt aaaaataaga tttccaaaat ttcacgcctc 120

aaaatctcat aataacttag aagtacaaat ttaaaatctt ctatgtattt aaatcatttc 180

aaacgatcta aaaatcgcgc gaattcttgt gacgtcatat gatcatattt tgtatgtcat 240

ttctaacgtc tcatttaaat tacatacaat tttgtttact ttggagttca aaaacacagt 300

ttttgaagag atagtaatga attgtgtgta tttttattat ggaaaataaa agtaaatact 360

atgaaagtgt tgtttaataa cattctgtta aattaaaacc attgaaactc ccaataaaat 420

atttattaga ttaccagcgg ataagaaacg gag 453

<210>427

<211>1529

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(874)

＜223〉n=a, c, g or t/u

<400>427

gagatatttg tagtatagtt ttgctttgaa gattcattat aggtttttaa ttatgattaa 60

tgaaatgatg gaactatcga acaattagaa tctgtatatc tttaggcaaa tctgtaaata 120

atcattgtag ttattcttgg aactagtttg caactaagaa atataattta agactttttg 180

aacacagtca aaagaccatg actatcacag atgtggatta gtaccacttt aaatttgtta 240

ataaaacata gaatttatag aatagtttcc ccgttttata ccgcatatgc gactttagga 300

ttatagcagg ctgtcatctc tagggctacg gtatacaaga aagataacag ggccagtgct 360

acgcttcaac cgcctattat tacccctggt tttagccaag gaactcattt tattcaggct 420

gagtcgacct ggggcctatt taaaaatgtc tagttgttct tgccggcgct gggtctcgaa 480

cccaggtcta ccagcaagcg agtcagacat cctaccgctg aactacgccg gcccatacgt 540

agagtttata attatcttat atataataaa ggaatttgat taaaaaaggt aaaaatacat 600

tgtattgggt tgcttaggcc taaatacata tctcttccta ttagtaagtt acaaaatggt 660

gagattcgat catttttgtg ggatatgtag ttgatgaatt aagtagtaag tagtagttga 720

atacacgtta cattttctca acattgatta atacatttat ttttttccaa aataagaaaa 780

atttagctac taaaaaaata tatgctaaat attgtttatc atcagctaaa agttaggaga 840

tctgttaaat cctataaaat atgaaaacaa tganaatgtg ttgtttcggt aagtacatag 900

tttgcagaca actggacgat acaagggatc taataacttt tagcaattga taaattgaac 960

gaaaaaatca caatgtttaa tcaaaagctc aaacacaaga acacaaagtt tttatgatgt 1020

taaataaatg aagactttct accgttcaca cgaaaggcgc ttatcctgat ctggttacat 1080

tttcttaaga taaaaccgtg ggtacataat atacacaatc aaacttatat ggaatcacca 1140

tgtatttcaa accaatattt atttcttttg aaaaaaactt gttaccaggc gcggatacag 1200

gggagggtca aggggtctat agacccccct attgtattta gtctataaga atttttttat 1260

tatttattat ttttttctgt caactctaaa ctttaagcca ccagtagaac actaaaatta 1320

aacgacaacc acttccaaag aattattgaa agaagcaata aaatctaata aaaccgagcc 1380

ttttctgaaa aataaattga agccgcattt gtttgggtac cgatggagcc gtaaacaact 1440

gaaatatttt tctcaattca aagaataacg gaaatgatat cttaaaatgc aaatactttt 1500

cgtgaggtaa ggtttatacc cggtgtaat 1529

<210>428

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>428

gtcaaaagac catgactatc acagatgtgg 30

<210>429

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>429

cgtccagttg tctgcaaact atgtacttac c 31

<210>430

<211>731

<212>DNA

<213>Diabrotica virgifera

<400>430

gtcaaaagac catgactatc acagatgtgg attagtacca cttcaaattt gttaataaaa 60

catagaattt atagaatagt ttccccgttt tataccgcat atgcgacttt aggattatag 120

caggctgtca tctctagggc tacggtatac aagaaagata acagggccag tgctacgctt 180

caaccgccta ttattacccc tggttttagc caaggaactc attttattca ggctgagtcg 240

acctggggcc tatttaaaaa tgtctagttg ttcttgccgg cgctgggtct cgaacccagg 300

tctaccagca agcgagtcag acatcctacc gctgaactac gccggcccat acgtagagtt 360

tataattatc ttatatataa taaaggaatt tgattaaaaa aggtaaaaat acattgtatt 420

gggttgctta ggcctaaata catatctctt cctattagta agttacaaaa tggtgagatt 480

cgatcatttt tgtgggatat gtagttgatg aattaagtag taagtagtag ttgaatacac 540

gttacatttt ctcaacattg attaatacat ttattttttt ccaaaataag aaaaatttag 600

ctactaaaaa aatatatgct aaatattgtt tatcatcagc taaaagttag gagatctgtt 660

aaatcctata aaatatgaaa acaatgaaat gtgttgtttc ggtaagtaca tagtttgcag 720

acaactggac g 731

<210>431

<211>1208

<212>DNA

<213>Diabrotica virgifera

<400>431

atattatttg ttctgtgaag cagctttgtt aggaaaaatc aaaatgtttt cagctacgtt 60

taaactgtta tttttgataa cagtaatatt ggtatccctt tcgccatcca gtgcagacaa 120

ttataacaaa tgtcctgaaa ccagccatat acgatcccta atttaccaga atacattgaa 180

ggggagagtt atacatgtac gcattccagg cacaggtttc tttgaagctc caatcacttg 240

tcttcaggtt accgatataa atgaaagttt cagtacacca gttgtagaag aaggtggata 300

tggaagggag tacgtagttt tacgtattga cccagttgaa tcaggagaac agttacaata 360

cgatgttcag atttatattg acgaagctgt tacagcctat cctcaataaa tgttcggtat 420

aaatgtttaa gaaattcgag tagccccttt aaagataagc agttgctggt attttaacag 480

cattactgat tgtaagttaa tcatttcaga ccttcacgta aactatgttg ttacaaaaac 540

gcatattagg acaataatcc aaattattag acaataaata ttaggcaaga gaaattaaac 600

gagtttctag ttttaagtac aaacaagtga agttcatcag caaaacagaa ttcagaatta 660

ttctacgtat tatattaaaa gcactatttg gttaaaagtt atcatttttg ggggtaaaag 720

aatctattaa tttgtcttga gtaaaggtag tatttgttta aatttgccga ccgatgatag 780

gtagattggg ccaagtacag tgggtgatca tattattaga gccacctaat atgttcagtg 840

ttttggagga atgtttacaa tgttttgtaa tttaatgttt acaatgttta gaatgtttac 900

attaaattac aaaaacgcat attaggacaa taatccaaat tattagacaa taaatattag 960

gcaagagaaa ttaaacgagt ttctagtttt aagtacaaac aagtgaagtt catcagcaaa 1020

acagaattca gaattattct acgtattata ttaaaagcac tatttggtta aaagttatca 1080

tttttggggg taaaagaatc tattaatttg tcttgagtaa aggtagtatt tgtttaaatt 1140

tgccgaccga tgataggtag attgggccaa gtacagtggg tgatcatatt attagagcca 1200

cctaatat 1208

<210>432

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>432

cctttcgcca tccagtgcag aca 23

<210>433

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>433

ctccaaaaca ctgaacatat taggtggctc 30

<210>434

<211>752

<212>DNA

<213>Diabrotica virgifera

<400>434

cctttcgcca tccagtgcag acaattataa caaatgtcct gaaaccagcc atatacgatc 60

cctaatttac cagaatacat tgaaggggag agttatacat gtacgcattc caggcacagg 120

tttctttgaa gctccaatca cttgtcttca ggttaccgat ataaatgaaa gtttcagtac 180

accagttgta gaagaaggtg gatatggaag ggagtacgta gttttacgta ttgacccagt 240

tgaatcagga gaacagttac aatacgatgt tcagatttat attgacgaag ctgttacagc 300

ctatcctcaa taaatgttcg gtataaatgt ttaagaaatt cgagtagccc ctttaaagat 360

aagcagttgc tggtatttta acagcattac tgattgtaag ctaatcattt cagaccttca 420

cgtaaactat gttgttacaa aaacgcatat taggacaata atccaaatta ttagacaata 480

aatattaggc aagagaaatt aaacgagttt ctagttttaa gtacaaacaa gtgaagttca 540

tcagcaaaac agaattcaga attattctac gtattatatt aaaagcacta tttggttaaa 600

agttatcatt tttgggggta aaagaatcta ttaatttgtc ttgagtaaag gtagtatttg 660

tttaaatttg ccgaccgatg ataggtagat tgggccaagt acagtgggtg atcatattat 720

tagagccacc taatatgttc agtgttttgg ag 752

<210>435

<211>1290

<212>DNA

<213>Diabrotica virgifera

<400>435

gttccagagg agtgcagaat gttttattag tgaagccttt aaacaggttt ttgtgtgaat 60

aaaaccggtg tatattattg tgaataaatg tgtcaaatat aggatatata cctgaaacag 120

attaactgca cttgtttaat aaaaggcact actacttact aacacaagat gtccgtattg 180

ggaatggtat cagccgtagc gggctttgtc aaagtccgct acttggttga taaagctcta 240

atcgacaata tggtgtttag agcccactat agagtcacgt cggccatatt gtttttatgc 300

tgtatcatcg ttactgcgaa taatctaatt ggtgacccca ttcagtgcat caacgacagg 360

ggtgttccag gtcatgtaat aaatacgttt tgctggataa cgcatacttt tacgttacca 420

catgaacaag gaaaacgcgt cgggacagaa gttgctcatc ctggtcttgg taacgaaaat 480

caggataaga gataccacag ctactaccaa tgggttccat tcgttttatt cttccagggc 540

gtcctctttt acatccctca ttggttgtgg aaaaattggg aacaaggaaa aattcgtatg 600

atcaccgaag gtatgagagg atcgatggtc ggagttgggg aaaaggcaga gagagaacat 660

agacagaata gactcgtaca atatcttatc gaaacgatgc acatgcacac ttcctatgca 720

ttcggatact ttatttgtga agctctaaac ttcgttaatg tgatggtaaa catattcatg 780

actgacaaat tcctcggtgg agcgttccta acctacggca ctgacgtagt ttccttttcg 840

aacatgaatc aagaaaatcg cacggatccc atggtagcca tctttccaag ggtaacaaaa 900

tgtacattcc acaaattcgg tccttcagga tcaattcaga agcatgacgc cctctgcgtg 960

ttagccttga acattctcaa cgagaagatt tacattttcc tatggttctg gtttattatt 1020

ttagctatac tgtccggttt ggctattgtg tattcggcag ctgttgtatt actacctagt 1080

acaagagaaa cgattttgaa gaggagattc agattcaagg tacccaatgc tgttgagact 1140

attgtcagaa aaacacaggt tggtgatttc cttttgattc acttactggg acaaaacaca 1200

aaccaattgg tatttggcga aattttggac gaattcgtaa gaagactgaa tttcggtagt 1260

aacagcaact taccgtctgc tcccagcacg 1290

<210>436

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>436

cacaagatgt ccgtattggg aatggtatc 29

<210>437

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>437

gtaagtgaat caaaaggaaa tcaccaacct g 31

<210>438

<211>1023

<212>DNA

<213>Diabrotica virgifera

<400>438

cacaagatgt ccgtattggg aatggtatca gccgtagcgg gctttgtcaa agtccgctac 60

ttggttgata aagctctaat cgacaatatg gtgtttagag cccactatag agtcacgtcg 120

gccatattgt ttttatgctg tatcatcgtt actgcgaata atctaattgg tgaccccatt 180

cagtgcatca acgacagggg tgttccaggt catgtaataa atacgttttg ctggataacg 240

catactttta cgttaccaca tgaacaagga aaacgcgtcg ggacagaagt tgctcatcct 300

ggtcttggta acgaaaatca ggataagaga taccacagct actaccaatg ggttccattc 360

gttttattct tccagggcgt cctcttttac atccctcatt ggttgtggaa aaattgggaa 420

caaggaaaaa ttcgtatgat caccgaaggt atgagaggat cgatggtcgg agttggggaa 480

aaggcagaga gagaacatag acagaataga ctcgtacaat atcttatcga aacgatgcac 540

atgcacactt cctatgcatt cggatacttt atttgtgaag ctctaaactt cgttaatgtg 600

attgtaaaca tattcatgac tgacaaattc ctcggtggag cgttcctaac ctacggcact 660

gacgtagttt ccttttcgaa catgaatcaa gaaaatcgca cggatcccat ggtagccatc 720

tttccaaggg taacaaaatg tacattccac aaattcggtc cttcaggatc aattcagaag 780

catgacgccc tctgcgtgtt agccttgaac attctcaacg agaagattta cattttccta 840

tggttctggt ttattatttt agctatactg tccggtttgg ctattgtgta ttcggcagct 900

gttgtattac tacctagtac aagagaaacg atttgaagag gagattcaga ttcaaggtac 960

ccaatgctgt tgagactatt gtcagaaaaa cacaggttgg tgatttcctt ttgattcact 1020

tac 1023

<210>439

<211>845

<212>DNA

<213>Diabrotica virgifera

<400>439

caaactcttt acaggttgag tatagcagtt taacagtatc ttcaacaagg tctcgagtta 60

catatgtttc gtgttaaagt gcagtgtaag tgaagttaca actttgcggt gcatcgccta 120

aaagtcatgg ctcttgtagc cagctcctgg cgagagacat ccttaggtaa cccggtgagc 180

ctgaactctt tgcccaacgg tttgaatcct ttacctcaga ctagtacttt aactccttta 240

cccaccaaca ttaatcctat taatcttgaa acatccgatc cttcgctgat caatcgacaa 300

acgccgtcac aaaactcaca aaatagtaat aacagtgtag ataagaacca gaacattgag 360

tgtgttgtgt gtggggacaa aagttctggt aaacactacg gacaattcac atgcgaagga 420

tgcaaatcat tttttaaaag gagtgttaga agaaatttaa cgtactcatg tcgagggagc 480

cgaaattgtc cgatagatca acatcataga aaccagtgcc aatattgtcg tttaaaaaaa 540

tgtatgaaaa tgggcatgcg gagagaagct gtgcagcgtg gcagagtacc accgtcgcag 600

ccttcgcttc caggtttacc aaatcagttc cttaactcaa cagactcgat gaatacatct 660

ctaagcaacc acacgtacct cagtagttac atatcactat tattaagagc agagccgtac 720

cctacgagtc gatatggtca atgtctgcaa agtaataaca ttatgggcat tgacaatata 780

tgcgagttag cagcgcggct gctattctca gcagtagaat gggcaaggaa tataccgttc 840

tttcc 845

<210>440

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>440

gctcctggcg agagacatcc ttagg 25

<210>441

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>441

gcagacattg accatatcga ctcgtagg 28

<210>442

<211>606

<212>DNA

<213>Diabrotica virgifera

<400>442

gctcctggcg agagacatcc ttaggtaacc cggtgagcct gaactctttg cccaacggtt 60

tgaatccttt acctcagact agtactttaa ctcctttacc caccaacatt aatcctatta 120

atcttgaaac atccgatcct tcgctgatca atcgacaaac gccgtcacaa aactcacaaa 180

atagtaataa cagtgtagat aagaaccaga acattgagtg tgttgtgtgt ggggacaaaa 240

gttctggtaa acactacgga caattcacat gcgaaggatg caaatcattt tttaaaagga 300

gtgttagaag aaatttaacg tactcatgtc gagggagccg aaattgtccg atagatcaac 360

atcatagaaa ccagtgccaa tattgtcgtt taaaaaaatg tatgaaaatg ggcatgcgga 420

gagaagctgt gcagcgtggc agagtaccac cgtcgcagcc ttcgcttcca ggtttaccaa 480

atcagttcct taactcaaca gactcgatga atacatctct aagcaaccat acgtacctca 540

gtagttacat atcactatta ttaagagcag agccgtaccc tacgagtcga tatggtcaat 600

gtctgc 606

<210>443

<211>932

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(650)..(836)

＜223〉n=a, c, g or t/u

<400>443

aaagagtatg tggtgtttaa tgttatttcg agtgggatta tctatcttat ttttctttca 60

tacaacatta gctcttcctg atgaatgtag ttctatttcg cctgtaagaa gttgtttact 120

gaggacaaac tacatagata tacggctgtg gagatattac gctggctatt tttgctacat 180

ttatacaaat gaagccaggt tggatatcaa atataattcc actagttaca acataacatg 240

tggcggtgaa aacgcaacga ttcccgtagc aagggtggag gagaacataa tagctagtta 300

tgattataca gataaagaag atgtccagca tatcagaaaa tcgtttaaga ctgacgaggt 360

ttttaaaaat tatatatctt gttgccaagg agctataagg tgttgcaatg atcacatgga 420

cgaggaaaat gttattccaa cgcaatcaca atgtcctgta atatgggatg cctggtcttg 480

ttttccacaa actaaagtta atactacagc caagctacca tgtagtagcc aagcatacca 540

atctcccgat gaagtctgta ctttagaaag cgaaaaacac tgcatctgga acggaacaaa 600

tcaaaaagca caatggatcc aacaaactga ctacacaacg tgtgccatan gcgccggttt 660

acgaacgaag atacaacttc catattatat ttctggggag tctgtatcgg cttttgtata 720

ccggcaatat ctatattttt ctttttcgaa aaacttcgaa agaccatcag agttattctg 780

cataggaacc ttttgatagc ccattgtgng tcaggaatgt cttgacaatt atgtgncaag 840

aactagtgct gctagacgct ttaaaagctt cggctctaag tcatcgtacg atggacaaca 900

atggagtccc atggtcggat attggctttt at 932

<210>444

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>444

gctcttcctg atgaatgtag ttctatttcg c 31

<210>445

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>445

cgtacgatga cttagagccg aagctt 26

<210>446

<211>817

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(627)

＜223〉n=a, c, g or t/u

<400>446

gctcttcctg atgaatgtag ttctatttcg cctgtaagaa gttgtttact gaggacaaac 60

tacatagata tacggctgtg gagatattac gctggctatt tttgctacat ttatacaaat 120

gaagccaggt tggatatcaa atataattcc actagttaca acataacatg tggcggtgaa 180

aacgcaacga ttcccgtagc aagggtggag gagaacataa tagctagtta tgattataca 240

gataaagaag atgtccagca tatcagaaaa tcgtttaaga ctgacgaggt ttttaaaaat 300

tatatatctt gttgccaagg agctataagg tgttgcaatg atcacatgga cgaggaaaat 360

gttattccaa cgcaatcaca atgtcctgta atatgggatg cctggtcttg ttttccacaa 420

actaaagtta atactacagc caagctacca tgtagtagcc aagcatacca atctcccgat 480

gaagtctgta ctttagaaag cgaaaaacac tgcatctgga acggaacaaa tcaaaaagca 540

caatggatcc aacaaactga ctacacaacg tgtgccatag cgccggttta cgaacgaaga 600

tacaacttcc atattatatt tctgggnagt ctgtatcggc ttttgtatac cggcaatatc 660

tatatttttc tttttcgaaa aacttcgaaa gaccatcaga gttattctgc ataggaacct 720

tttgatagcc attgtggtca ggaatgtctt gacaattatg tgcaaagaac tagtgctgct 780

agacgcttta aaagcttcgg ctctaagtca tcgtacg 817

<210>447

<211>880

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(737)..(860)

＜223〉n=a, c, g or t/u

<400>447

ccaaattcag atttggttgg aggagataat tttgaagcct ctaaatattt aaggatactt 60

caacaggcaa tgaatgaaga tgaagaagga ttagtagaaa gacatagagg aatctatgga 120

ttgaaagtag tgaaaaaatc aggtgaaact ggatattggg tgatcaatac taaggtaggc 180

aaaggacaga tagtatttaa tggaaaagac aagccagatg ttactttcat tgttaaggaa 240

gaggatgttt tagaattatt gtctggcaaa attccaccac agaaggcatt tttccaaggc 300

aaagtaaaaa ttcaagggaa tatgggtgta gccatgcgat taatagaatt acagaaaaca 360

gccgctgcca aaattgaggt acttagggct aagttataaa ttacgtttat aggtaatttt 420

ctttatcttt ctgtattgtg tacactaagg ttttgtttta tctttttggg agtatatatt 480

atataagtga caaaacatac tttcttgttt tacacatcac ttcctgtaaa aatattgtac 540

acgctgtaca atgagtcctt tatgtaaaca aatatgtaag tataaatact tgctctgtta 600

tctccttttt attacatatt tttgttgata acttcagaat ctattgacct tttgtgaaat 660

actttcaata ttttgttaac agttgaagac cagtgtaatt tttcctgaga tactaaattc 720

aaatattttt gtttcanaca agtttgttcc gtaaataaac tagacatgta tatcattgta 780

tattattaat aatatgaaac cttgatgtct gtaggtaaat ggtcttaatg atttaatggt 840

ttttcataat ncatagtccn aagtcacaaa atagcatata 880

<210>448

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>448

ggcaat gaat gaagat gaag aaggattagt 30

<210>449

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>449

ccattaaatc attaagacca tttacctaca gaca 34

<210>450

<211>774

<212>DNA

<213>Diabrotica virgifera

<400>450

ggcaatgaat gaagatgaag aaggattagt agaaagacat agaggaatct atggattgaa 60

agtagtgaaa aaatcaggtg aaactggata ttgggtgatc aatactaagg taggcaatgg 120

acagatagtg tttaatggaa aagacaagcc agatgttact ttcattgtta aggaagagga 180

tgttttagaa ttattgtctg gcaaaattcc accacagaag gcatttttcc aaggcaaagt 240

aaaaattcaa gggaatatgg gtgtagccat gcgattaata gaattacaga aaacagccgc 300

tgccaaaatt gaggtactta gggctaagtt ataaattacg tttataggta attttcttta 360

tctttctgta ttgtgtacac taaggttttg ttttatcttt ttgggagtat atattatata 420

agtgacaaaa catactttct tgttttacac atcacttcct gtaaaaatat tgtacacgct 480

gtacaatgag tcctttatgt aaacaaatat gtaagtataa atacttgctc tgttatctcc 540

tttttattac atatttttgt tgataacttc ggaatctatt gaccttttgt gaaatacttt 600

caatattttg ttaacagttg aagaccagtg taatttttcc tgagatacta aattcaaata 660

tttttgtttc aaacaagttt gttccgtaaa taaactagac atgtatatca ttgtatatta 720

ttaataatat gcaaccttga tgtctgtagg taaatggtct taatgattta atgg 774

<210>451

<211>576

<212>DNA

<213>Diabrotica virgifera

<400>451

aaattatcgg catatattat gcaggataac caactacatg ctaatctaag agtggatgaa 60

gctatggctg tcgcagctgc acttaaaatt ggcgccaaaa gtccaaaaga tcgagaagat 120

atcattaacg aaatcctaga cacacttgga ctgctggatc ataagaaaac aatgacaagc 180

gggttgtcgg ggggccaaaa gaagagattg tcaatcgcct tagaattagt tagtaatcct 240

ccagttatgt ttttcgatga acctactagt ggattggatt cttcatcatg ttttcaatgt 300

atatcactgt tgaaaacctt agctaaaggt ggaagaacta taatatgtac tattcaccaa 360

ccttcagcta gactgtttga aatgttcgac caactttata cactcgctga tggtcaatgt 420

gtttaccaag gttctacgac attcttggtg ccctttttgg caagtctgca gttacagtgt 480

ccttcatacc acaatccagc ctcctatatt atagaagtag catgtgggga atatggagat 540

cacacgagaa cgttagttaa cgctatagaa aatggt 576

<210>452

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>452

ggataaccaa ctacatgcta atctaagagt ggat 34

<210>453

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>453

ggaggctgga ttgtggtatg aaggac 26

<210>454

<211>482

<212>DNA

<213>Diabrotica virgifera

<400>454

ggataaccaa ctacatgcta atctaagagt ggatgaagct atggctgtcg cagctgcact 60

taaaattggc gccaaaagtc caaaaagatc gagaagatat cattaacgaa atcctagaca 120

cacttggact gctggatcat aagaaaacaa tgacaagcgg gttgtcgggg ggccaaaaga 180

agagattgtc aatcgcctta gaattagtta gtaatcctcc agttatgttt ttcgatgaac 240

ctactagtgg attggattct tcatcatgtt ttcaatgtat atcactgttg aaaaccttag 300

ctaaaggtgg aagaactata atatgtacta ttcaccaacc ttcagctaga ctgtttgaaa 360

tgttcgacca actttataca ctcgctgatg gtcaatgtgt ttaccaaggt tctacgacat 420

tcttggtgcc ctttttggca agtctgcagt tacagtgtcc ttcataccac aatccagcct 480

cc 482

<210>455

<211>759

<212>DNA

<213>Diabrotica virgifera

<400>455

cccacgcgtc cggtactaca tgtatggtac tgctttagtt gtgttttgac agattcgttc 60

aataatatca tttcttttat ttatttatat attaatcttt gaagatgttg tctggtggct 120

atatattgga attgtgcaat gttttttatt ctggacaggt aaaaaagcgc tctgttcttc 180

aaagaatggt tggcaacatt aaaactgcag taatcttaaa agatgtgtcc atgttggtac 240

attctggtga ggtcctagca gtgctgggat caaaaggtag tgggaagaaa gccttacttg 300

atgttatttc aagacgagca caaggcccta taagaggaca gatatatctg aacaaccatc 360

cagtcagtat gggtttattc cagcagaaat gtgcatatgt tacacataaa tgtgatttta 420

taccaggact aaatgttgaa cagacgttgt attatactcc gactaagttc actggctatt 480

taaaaatgtc taaagtaaag caagtgatag cagatttagc attgtcacaa gtcgctaaaa 540

aatgcgttga agatttgaca aaaagtgaat acagaagact gatgattggt gtccaactaa 600

tcaaaaaccc agtggtacta ttactagacg aacccacctg ggatctggac ccactaaaca 660

catacctaat aatttcaata ctttcaaatg cagctaaaaa gtatggaact gctataatct 720

tgacgatgga gaagcctaga tctgatgttt tcccgttct 759

<210>456

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>456

ggtactgctt tagttgtgtt ttgacagatt cg 32

<210>457

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>457

gcttctccat cgtcaagatt atagcagttc c 31

<210>458

<211>710

<212>DNA

<213>Diabrotica virgifera

<400>458

ggtactgctt tagttgtgtt ttgacagatt cgttcaataa tatcatttct tttatttatt 60

tatatattaa tctttgaaga tgttgtctgg tggctatata ttggaattgt gcaatgtttt 120

ttattctgga caggtagaaa agcgctctgt tcttcaaaga atggttggca acattaaaac 180

tgcagtaatc ttaaaagatg tgtccatgtt ggtacattct ggtgaggtcc tagcagtgct 240

gggatcaaaa ggtagtggga agaaagcctt acttgatgtt atttcaagac gagcacaagg 300

ccctataaga ggacagatat atctgaacaa ccatccagtc agtatgtgtt tattccagca 360

gaaatgtgca tatgttacac ataaatgtga ttttatacca ggactaaatg ttgaacagac 420

gttgtattat actccgacta agttcactgg ctatttaaaa atgtctaaag taaagcaagt 480

gatagcagat ttagcattgt cacaagtcgc taaaaaatgc gttgaagatt tgacaaaaag 540

tgaatacaga agactgatga ttggtgtcca actaatcaaa aacccagtgg tactattact 600

agacgaaccc acctgggatc tggacccact aaacacatac ctaataattt caatactttc 660

aaatgcagct aaaaagtatg gaactgctat aatcttgacg atggagaagc 710

<210>459

<211>837

<212>DNA

<213>Diabrotica virgifera

<400>459

cttaaaaggg agagatctct tcgaggaaaa aaaagtaggt ctctattccg acgaaacgaa 60

agcagtgccc gcagcaatca ttatgcagtt tttgttattg tataagagga atctgctgat 120

aataaagaga tgttacggtc catcgctgaa cagagtattg gcccacattg ttattggtct 180

gatctttggc tatttgtata gaaacgttgg aagtgccgcg gatacagttt tagcaaatta 240

tgtatatttg tatggtactc ttttactcac agtatatact ggtaaaatgc ccgtaacttt 300

atcatttcct ctagaaatga agatactctc tagagagcat tttaaccgtt ggtataagct 360

aactccatat ttattgtctg tgatattagt tgaaattcct ttccaagtta tatgtacatg 420

gatgtacata gcagtatctt attcgttaac gaaccagcca ttagacttta ggttattctt 480

gtttgtcatt tttgtaacag cgtgttctct ctgtggccaa tccatgggat attttatagg 540

agccacaaca ccagtaaagg tagctgtatt catcgcccca gtgttagcct gcttcttatc 600

agtcttcggt ttttgcatca gagcaataga cacctctacc atgttcaaac cgatcttctt 660

catcagttac tttagagcag cattccaaag cgtcgtttat agtatttacg gctttaaccg 720

agagatattg gtttgcccaa tagaggagga atactgtcat tataaagatc ctcacaagtt 780

cctctcggaa atgaaatttt agatgtagat tttagttcaa attttatatt gattggt 837

<210>460

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>460

ggtctctatt ccgacgaaac gaaagcag 28

<210>461

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>461

cgtaaatact ataaacgacg ctttggaatg c 31

<210>462

<211>673

<212>DNA

<213>Diabrotica virgifera

<400>462

ggtctctatt ccgacgaaac gaaagcagtg cccgcagcaa tcattatgca gtttttgtta 60

ttgtataaga ggaatctgct gataataaag agatgttacg gtccatcgct gaacagagta 120

ttggcccaca ttgttattgg tctgatcttt ggctatttgt atagaaacgt tggaagtgcc 180

gcggatacag ttttagcaaa ttatgtatat ttgtatggta ctcttttact cacagtatat 240

actggtaaaa tgcccgtaac tttatcattt cctctagaaa tgaagatact ctctagagag 300

cattttaacc gttggtataa gctaactcca tatttattgt ctgtgatatt agttgaaatt 360

cctttccaag ttatatgtac atggatgtac atagcagtat cttattggtt aacgaaccag 420

ccattagact ttaggttatt cttgtttgtc atttttgtaa cagcgtgttc tctctgtggc 480

caatccatgg gatattttat aggagccaca acaccagtaa aggtagctgt attcatcgcc 540

ccagtgttag cctgcttctt atcagtcttc ggtttttgca tcagagcaat agacacctct 600

accatgttca aaccgatctt cttcatcagt tactttagag cagcattcca aagcgtcgtt 660

tatagtattt acg 673

<210>463

<211>660

<212>DNA

<213>Diabrotica virgifera

<400>463

ttcgtccgaa atcatctagt gtggttcgac tacgcgttta tactaagtaa tctgaagtgg 60

ctggacattc acggtaactt tattgaacat ctcgctaatt attacaagat tcaagacgta 120

atcagagtca aaactttaga tgctagtcat aatcgtatta ccgaaatttc tcctatgtct 180

attcctaata gtatagaact tttatttatt aataacaatt ttatcaaatc ggtgcaacca 240

aatactttcc tcgataaatc aaacttagct cgtgtcgata tgtatgctaa cgacatgaca 300

aatttagatt taaattctct gagaatcgct ccagttcatt cgaaccgttc cttaccagaa 360

atatatcttg gtggcaatac ctttcattgt gactgtacta tggagtggct acagcttatc 420

aacaacatgt cagcatccag gcagtatcct cgtgtaatgg acatggacaa cattgtatgc 480

aaaatgacac attctagagg tatgacacat atgccattaa atcaggcaaa acaaagtgac 540

tttctttgta actacgaaac acattggttt gctctttgcc attgctggga ttttgatgca 600

tgtgattgtg aaatgacttg tcctaataat tggacttgtt accacgatca gacgtggaat 660

<210>464

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>464

ctaagtaatc tgaagtggct ggacattcac g 31

<210>465

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>465

gcaatggcaa agagcaaacc aatgtg 26

<210>466

<211>543

<212>DNA

<213>Diabrotica virgifera

<400>466

ctaagtaatc tgaagtggct ggacattcac ggtaacttta ttgaacatct cgctaattat 60

tacaagattc aagacgaaat cagagtcaaa actttagatg ctagtcataa tcgtattacc 120

gaaatttctc ctatgtctat tcctaatagt atagaacttt tatttattaa taacaatttt 180

atcaaatcgg tgcaaccaaa tactttcctc gataaatcaa acttagctcg tgtcgatatg 240

tatgctaacg acatgacaaa tttagattta aattctctga gaatcgctcc agttcattcg 300

aaccgttcct taccagaaat atatcttggt ggcaatacct ttcattgtga ctgtactatg 360

gagtggctac agcttatcaa caacatgtca gcatccaggc agtatcctcg tgtaatggac 420

atggacaaca ttgtatgcaa aatgacacat tctagaggta tgacacatat gccattaaat 480

caggcaaaac aaagtgactt tctttgtaac tacgaaacac attggtttgc tctttgccat 540

tgc 543

<210>467

<211>894

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(745)..(784)

＜223〉n=a, c, g or t/u

<400>467

cgtaaataag attcttgtgt tattatttct tcctttttgc ttcggcaaaa gtgtagaagt 60

gacttttaaa gatgtcacgt tcaaaggctt tagtgttttt acagaaaagt tcaagaaaaa 120

gatcccatct ggaaacaaac tagcagattt tttgcccccg aaaactgttt atgatgccat 180

tgaaatgaag gaacaacata tacctgtact tgccaaaggc tcgttggcag atctagacca 240

gttggacgaa ttggttattg agtactgtgg tgttgaagaa gttgaatctg gagctatcag 300

taatgtacct aacctaagaa aactgtcact taaaggtaat gccatcaagt cgattgcaaa 360

ggacgtattt tcccatttag gaatatctac actagacctt agcctcaatc agatatcaac 420

aatccatcca caggcctttg acaacatgcc ggatctctta aacattcaac ttgctgacaa 480

ttttataacg caatggaatc cagaatggct caagaataca ccgctattga ccaggatctc 540

gcttcagaat aattccatag aaaagctacc agcccatgca tttaaaaaca tggtaggaga 600

taaaaagtat ggaaaaatag acctcactat taatctggta tttagtcata ataaaataag 660

tgagatagat cctgaagctt tcgcggatct cagcagaatt aacaacttgt ggttagataa 720

caatgttcta gaaagtttcg atganaacct tttatcggga ataaaagtta atgatttgag 780

actncacaaa aataatatca aatgtttaga tggtaatcta gataaaatac taaaagcaga 840

aacgaacaca tagattcgaa tccgtcgact gtaattgctt gagaagatca agga 894

<210>468

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>468

gatgccattg aaatgaagga acaaca 26

<210>469

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>469

ctaaccacaa gttgttaatt ctgctgagat cc 32

<210>470

<211>544

<212>DNA

<213>Diabrotica virgifera

<400>470

gatgccattg aaatgaagga acaacatata cctgtactta ccaaaggctc gttggcagat 60

ctagaccagt tggacgaatt ggttattgag tactgtggtg ttgaagaagt tgaatctgga 120

gctatcagta atgtacctaa cctaagaaaa ctgtcactta aaggtaatgc catcaagtcg 180

attgcaaagg atgtattttc ccatttagga atatctacac tagaccttag cctcaatcag 240

atatcaacaa tccatccaca ggcctttgac aacatgccgg atctcttaaa cattcaactt 300

gctgacaatt tcataacgca atggaatcca gaatggctca agaatacacc gctattgacc 360

aggatctcgc ttcagaataa ttccatagaa aagctaccag cccatgcatt taaaaacatg 420

gtaggtgata aaaagtatgg aaaaatagac ctcactatta atctggtatt tagtcataat 480

aaaataagtg agatagatcc tgaagctttc gcggatctca gcagaattaa caacttgtgg 540

ttag 544

<210>471

<211>210

<212>DNA

<213>Diabrotica virgifera

<400>471

gagcgctgac ataaccaaaa agcgcaagcg ccaccacaca aaaaaccgtc ttcattttcc 60

tgagtcgaac gaacctttca caactcaccg tactaatggt actatggtac tagctagcga 120

atgccaaata caaacacggg ctctttgccg gcaaaagact tcttcctcac tgaagatatc 180

ttgtaagttc ttcctctttt caaacgggcg 210

<210>472

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>472

gctgacataa ccaaaaagcg caagc 25

<210>473

<211>33

<212>DNA

<213>Diabrotica virgifera

<400>473

ccgtttgaaa agaggaagaa cttacaagat atc 33

<210>474

<211>203

<212>DNA

<213>Diabrotica virgifera

<400>474

gctgacataa ccaaaaagcg caagcgccac cacacaaaaa accgtcttca ttttcctgag 60

tcgaacgaac ctttcacaac tcaccgtact aatggtacta tggtactagc tagcgaatgc 120

caaatacaaa cacgggctct ttgccggcaa aagacttctt cctcactgaa gatatcttgt 180

aagttcttcc tcttttcaaa cgg 203

<210>475

<211>1139

<212>DNA

<213>Diabrotica virgifera

<400>475

tatttttttt attcttaagt tactgaaaaa attttggcgt gaatacttat gatctttttg 60

tgaaaaagat cttactgtat attttaaata tgtaatttac ttaactcatt cgctgcctgt 120

caagaaaatg gaactcggct agggccgttc tgttaaaagg cgcctgactg attggcgcgt 180

gaatggcacc tgactgaagt aaccatatca cgcgctggag tgtgatcgtc agcgaatggg 240

ttaaatgaca agtggttata gatgtttata cagtctaaat atatacagtg agttaaatat 300

tgggtcgagc tctccgtggg taataataat tattggttca aaaggatcca aacttgtcac 360

gggttagctc tgccggacaa tagattcagt aaaacacagg tttaatttaa ataaaaatat 420

atttttgtaa tccaatacaa taaaataaca acaaaatagt attcaattct ccgactattg 480

tctccctttt ggcacccata tcgagccgct gttgatgaaa tcgggtctca agatatcgtt 540

ggtaagttct ctgcatagtc gaatgattca cattcaattg tcttgccaca tacctttggt 600

ttgctccatt ttccgggaat gtaaccatct gaacacactt tctatacttt ttcatcgtta 660

taaaaataga cttcactgtc aggaggccag tgtaccacga agatatctca gaaactcttt 720

agaagactac aacagttcac acagttctta aaaccatcaa ttctgcgaag cttcgttgat 780

tcgtaaataa cgattggcac ccatatcgag ccgctgttga tgaaatcggg tctcaagata 840

tcgttggtaa gttctctgca tagtcgaatg attcacattc aattgtcttg ccacatacct 900

ttggcttgct ccattttccg ggaatgtaac catctgaaca cactttctat actttttcat 960

cgttataaaa atagacttca ctgtcaggag gccagtgtac cacgaagata tctcagaaac 1020

tctttagaag actacaacag ttcacacagt tcttaaaacc atcaattctg cgaagcttcg 1080

ttgattcgta aataacgatt ggcacccata tcgagccgct gttgatgaaa tcgggtctc 1139

<210>476

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>476

ctcattcgct gcctgtcaag aaaatg 26

<210>477

<211>31

<212>DNA

<213>Diabrot ica virgifera

<400>477

gcagagaact taccaacgat atcttgagac c 31

<210>478

<211>444

<212>DNA

<213>Diabrotica virgifera

<400>478

cgctgcctgt caagaaaatg gaactcgcct agggccgttc tgttaaaagg cgcctgactg 60

attggcgcgt gaatggcacc tgactgaagt aaccatatca cgcgctggag tgtgatcgtc 120

agcgaatggg ttaaatgaca agtggttata gatgtttata cagtctaaat aaatacagtg 180

agttaaatat tgggtcgagc tctccgtggg taataataat tattggttca aaaggatcca 240

aacttgtcac gggttagctc tgccggacaa tagattcagt aaaacacagg tttaatttaa 300

ataaaaatat atttttgtaa tccaatacaa taaaataaca acaaaatagt attcaattct 360

ccgactattg tctccctttt ggcacccata tcgagccgct gttgatgaaa tcgggtctca 420

agatatcgtt ggtaagttct ctgc 444

<210>479

<211>851

<212>DNA

<213>Diabrotica virgifera

<400>479

actttaacgc ctagtgtcga gtctcgtgtg gcatatttca acagatactc aaatattgtc 60

atctaagaag ctggaacagg gatagtcact acgggcataa ctgcacaagc ttgagaaggc 120

taaagaaacc taactggtgt cgcctgatac caggaattgg gtatactctc catctatatt 180

tgtttgcgcc tttcaagtag acttgcacct agtctcgttt ggcttctttc aatagatact 240

caaacctttt cgtctaagca ccacaaatgc acaccctttg gacatttttc ggccaatgtt 300

gagctctacc acggttttct gttctgttcc tattctattc ttgccccaca agaagctggg 360

gaattgatga tctctacggc ataccctcac atgcgggaaa aggctaaaga gatctatgtg 420

gtatcacctg caagatcttt ttgtggatac cctgcttctt ttagaattaa agcgtttatt 480

tgaggctttt ggatagactt taacgcctct agagcttttt tgttttgttt caacagatac 540

tcaaaccttt tccaaattga acttcttagc agttttttta ttcgttccta gtctacacat 600

gtcccaggag aagttactga gtcgccccta gcttcaactc gaccgtgcgc accttataaa 660

tggcgcgtat acgtataatc ctttcccggg cctaccacat cgaataaaaa tgtttcttga 720

ttgatcgatt acatgtttct tacattgtct gtgtatatgt tgtaaatgct tgtttgtttg 780

atgtgcctaa atccgttcta tgtataaata cattcctaga tacttcttat gagtgatgaa 840

atcatagtga t 851

<210>480

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>480

ctttaacgcc tagtgtcgag tctcgtg 27

<210>481

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>481

gaacggattt aggcacatca aacaaac 27

<210>482

<211>797

<212>DNA

<213>Diabrotica virgifera

<400>482

ctttaacgcc tagtgtcgag tctcgtgtgg catatttcaa cagatactca aatattgtca 60

tctaagaagc tggaacaggg atagtcactg cgggcataac tgcacaagct tgagaaggct 120

aaagaaacct aactggtgtc gcctgatacc aggaattggg tatactctcc atctatattt 180

gtttgcgcct ttcaagtaga cttgcaccta gtctcgtttg gcttctttca atagatactc 240

aaaccttttc gtctaagcac cacaaatgca caccctttgg acatttttcg gccaatgttg 300

agctctacca cggttttctg ttctgttcct attctattct tgccccacaa gaagctgggg 360

aattgatgat ctctacggca taccctcaca tgcgggaaaa ggctaaagag atctatgtgg 420

tatcacctgc aagatctttt tgtggatacc ctgcttcttt tagaattaaa gcgtttattt 480

gaggcttttg gatagacttt aacgcctcta gagctttttc gttttgtttc aacagatact 540

caaacctttt ccaaattgaa cttcttagca gtttttttat tcgttcctag tctacacatg 600

tcccaggaga agttactgag tcgcccctag cttcaactcg accgtgcgca ccttataaat 660

ggcgcgtata cgtataatcc tttcccgggc ctaccacatc gaataaaaat gtttcttgat 720

tgatcgatta catgtttctt acattgtctg tgtatatgtt gtaaatgctt gtttgtttga 780

tgtgcctaaa tccgttc 797

<210>483

<211>1612

<212>DNA

<213>Diabrotica virgifera

<400>483

ctcagctatg atatatattt cttaataaat cttatagtat caacacatgg attcttagaa 60

ctactcttat atgactaaat ttttacataa tctctaaaaa tcccagcatt tcatgctttt 120

ctcttcatac cctttcttcc cagcttcagg cactataatt atttactgta agatttcgca 180

ttaatgatat agagcatttt ttatatgaac cataaaaaga tcagtatgtt taaaatagtg 240

tacctcgatg gttttgatgg ttttttcgtt tgttggattg attgtttaaa aatattattt 300

gttttggtat tggtttcaat gtgacaaaag tcaagaatca agcatttttg ttgtatccac 360

agtaggaata gtatagcaaa caatattcaa gtatttttat ttaaaatgaa aaattatttt 420

tagcttttta ggtcacctgt tggcaaataa ccttgaaaat gtagaacata ttgctgatga 480

attatgattc tcatatttgt tttttatgag tcaaaatacg ccgtaatatt ataacaatat 540

tatttattta aattattata aaatcagaag attttcatta aatgatgaat taggattgaa 600

cgagctgtga taaattaccc gaaaatatcc acaatgttgc caaaagcaac attttggaca 660

cagtaatgcg acattacagg atgtattatt ttaactgtaa aaggattgta ttcagacaaa 720

ttaatgatag ggcttgtcca gatagatcca tttatatctg ttcgaacagt caagcacctc 780

taaaggctct ggaagcaccc aagcaagtca gctctaagtt gattcttgaa tgcaagatag 840

cattgtctga ttggtcagag gtgaaagatc ctggattcga cctttacgca accgagctca 900

atgaaggagt tacctctaaa taattgtggc tttctgtgag accacaagtt tattaaagca 960

ttagacgtga ggaacggcac gaaagagctc atatttagag accctgctgg ttctagcaga 1020

tatgggagct cgaaatagcc tggaccccag aaacggcgaa gagtatgttc ttatgggcat 1080

agtctgggcg ataggagcga cacaactgac ccgccttaga atgtctaaag ggctcccaaa 1140

tgggtccttc cccgatctac catgacatta ttaacattgt gcttttggta aaaagtacgc 1200

tacacaacat aatattttaa attctgctta gcgccaatgt tagggggacc taatatgaca 1260

cagaaataag cttatcgttc aaatctcggc accaacgaca ttttcatttc tacaaatgat 1320

acgagccgtg caccgtactt cggaggacac gaaaagccgt cggtcccctt gggctagtgt 1380

gcattgacac tagctacttg aaacagggtt aaagctgcaa ttgaccggaa gctgtccgaa 1440

aggatcaccc cggtaaaaat accatacgat attattatat aatcatacaa aaacgctata 1500

tatcaataat acgaaaattt cttatgtaaa atgttttcca aaatatttct attatactct 1560

taattataag acattctaaa ttttgcgaaa taaactttaa gctctgctta tc 1612

<210>484

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>484

ctcttcatac cctttcttcc cagcttca 28

<210>485

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>485

gcacaatgtt aataatgtca tggtagatcg 30

<210>486

<211>1081

<212>DNA

<213>Diabrotica virgifera

<400>486

ctcttcatac cctttcttcc cagcttcagg cactataatt atttactgta agatttcgca 60

ttaatgatat agagcatttt ttatatgaac cataaaaaga tcagtatgtt taaaatagtg 120

tacctcgatg gttttgatgg ttttttcgtt tgttggattg attgtttaaa aatattattt 180

gttttggtat tggtttcaat gtgacaaaag tcaagaatca agcatttttg ttgtatccac 240

agtaggactg gaatagtata gcaaacaata ttcaagtatt tttatttaaa atgaaaaatt 300

atttttagct ttttaggtca cctgttggca aataaccttg aaaatgtaga acatattgct 360

gatgaattat gattctcata tttgtttttt atgagtcaaa atacgccgta atattataac 420

aatattattt atttaaatta ttataaaatc agaagatttt cattaaatga tgaattagga 480

ttgaacgagc tgtgataaat tacccgaaaa tatccacaat gttgccaaaa gcaacatttt 540

ggacacagta atgcgacatt acaggatgta ttattttaac tgtaaaagga ttgtattcag 600

acaaattaat gatagggctt gtccagatag atccatttat atctgttcga acagtcaagc 660

acctctaaag gctctggaag cacccaagca agtcagctct aagttgattc ttgaatgcaa 720

gatagcattg tctgattggt cagaggtgaa agatcctgga ttcgaccttt acgcaaccga 780

gctcaatgaa ggagttacct ctaaatataa ttgtggcttt ctgtgagacc acaagtttat 840

ttaagcatta gacgtgagga acggcacgaa agagctcata tttagagacc ctgctggtcc 900

tagcagatat gggagctcga aatagcctgg accccagaaa cggcgaagag gggtttatgg 960

tttattttct taggggcata gtctggacga taggagcgac acaactgacc cgccttagaa 1020

tgtctaaagg gctcacaaat gggtcctttc ccgatctacc atgacattat taacattgtg 1080

c 1081

<210>487

<211>919

<212>DNA

<213>Diabrotica virgifera

<400>487

ccctcactta tttactaaga ttccatcata tttctaagcg ataaaattta gggaagaatt 60

tatgaaatgt catcttaaaa cgtgagagca aattggcaca aacttgagcg ttgaatcagt 120

agtggataaa catcattcga atgatgaatt tgttttattc tcacctgtaa aaaaaacaaa 180

gtgtaaatag tataagatta agtgtatatt gatattagaa ataaatggcg gcgtaccaat 240

tttctaaaag aaggagttct tcaatcaaaa aggaattcaa cgagagtgag ctagaaggtc 300

tgaaaattgc ttttgagctg ctggatcgga accaagatgg acaggtcaca ccgggagagt 360

tcaagatcat gctgaataat ttaggaattg acattaagta ttaaaaaagc tgaagagata 420

attaggattg ccagtcattc aggctcagac ttaatagacc aaaacgactt cctttctttc 480

gttaaacaga ttcaaaaagc atgtccagga gacgtagacg aggatatagc tagtgaccta 540

aaggcagcct ttcaagtttt tgatttggac ggagatggtt atattacaaa agaggaatta 600

aaaactgcca tggaaatgat tggggaagct gttacagatc aacaattgga acaggttatt 660

cgactggctg atactgataa ggatggaagg ataaactatg aagaatttgt taagttatta 720

acatcgtaaa gagagtaaga agaacaaagc ttaaaatggt tccaatgttc tatggtaaat 780

atttggtttt aagtaaaagc caggctgtga tccatgttta caattaaata tcattaagca 840

aacatatttt gactgaattg agaaatattt aatggatatt tttgtttcct aagtagatac 900

ctaggtaatt attgtaaag 919

<210>488

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>488

cttgagcgtt gaatcagtag tggataaaca t 31

<210>489

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>489

ccttccatcc ttatcagtat cagccag 27

<210>490

<211>589

<212>DNA

<213>Diabrotica virgifera

<400>490

cttgagcgtt gaatcagtag tggataaaca tcattcgaat gatgaatttg ttttattctc 60

acctgtaaaa aaaaaacaaa gtgtaaatag tataagatta agtgtatatt gatattagca 120

ataaatggcg gcgtaccaat tttctaaaag aaggagttct tcaatcaaaa aggaattcaa 180

cgagagtgag ctagaaggtc tgaaaattgc ttttgagctg ctggatcgta accaagatgg 240

acaggtcaca ccgggagagt tcaagatcat gctgaataat ttaggaattg acattaagta 300

tgaaaaagct gaagagataa ttaggattgc cagtcattca ggctcagact taatagacca 360

aaacgacttc ctttctttcg ttaaacagat tcaaaaagca tgtccaggag acgtagacga 420

ggatatagct agtgacctaa aggcagcctt tcaagttttt gatttggacg gagatggtta 480

tattacaaaa gaggaattaa aaactgccat ggaaatgatt ggggaagctg ttacagatca 540

acaattagaa caggttattc gactggctga tactgataag gatggaagg 589

<210>491

<211>914

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(833)..(862)

＜223〉n=a, c, g or t/u

<400>491

gttaaaaaaa acgctccctg acgctcttgc ttccggttgc accaaatgca acataaaaca 60

aaaagacacg gctgagaagg taatgaaaca tcttatgtca aggaggccga aagactggga 120

cagacttacc aagaagtatg atccacaagg aaactttaaa aaacgttacg aggaacactt 180

gcaaaaagaa aagaaagcgg cttagaaaat tatctttaaa gaagatgaaa agtcaaacat 240

tttgttgtgt ttaataattt tattgtaatt tttttttcac ttatagggga gtgcaattag 300

aacgaaaaga tacaatgttt cggaaaaaat caaacaaggt tatatttttc taaaactttt 360

tttgttagtt tataaatatg ttaaagtaaa aacttctact cacaaatttt gccgctaatt 420

gtttattaat tgtttaaaca ataacaattg ctttgtataa ataatgttaa gaatatgggt 480

gaattcaaca ttttattttg ataaaaaatg tttttatttt agttttgatt atgctgaacc 540

cgaatctgac attacaattt gcaaattcaa aatggcggat tttttcctaa aaatccttaa 600

aaagtagttg taaaatccga ttttttttta taaaatgtgt ttgtttgcat attatatgtg 660

gatatttttg agaggttgct gaacctgaat aaaattttga taatttaaat tataaaatgg 720

cagatccaaa atggcggata gcttaaaaaa tagttgtaaa atcataattt ctttacaaga 780

tgtatttatt tctacatata tttatttttg agtgctttga taaacctaac ttnnaatgta 840

acattataaa ctanntaaat gncggatcca aaatgcggct tttcataaaa gaacataaaa 900

aagacatttt tcta 914

<210>492

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>492

cggctgagaa ggtaatgaaa catcttatg 29

<210>493

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>493

gttcagcaac ctctcaaaaa tatccaca 28

<210>494

<211>616

<212>DNA

<213>Diabrotica virgifera

<400>494

cggctgagaa ggtaatgaaa catcttatgt caaggaggcc gaaagactgg gacagactta 60

ccaagaagta tgatccacaa ggaaacttta aaaaacgtta cgaggaacac ttgcaaaaag 120

aaaagaaagc ggcttagaaa attatcttta aagaagatga aaagtcaaac attttgttgt 180

gtttaataat tttattgtaa tttttttttc acttataggg gagtgcaatt agaacgaaaa 240

gatacaatgt ttcggaaaaa atcaaacaag gttatatttt tctaaaactt tttttgttag 300

tttataaata tgttaaagta aaaacttcta ctcacaaatt ttgccgctaa ttgtttatta 360

attgtttaaa caataacaat tgctttgtat aaataatgtt aagaatatgg gtgaattcaa 420

cattttattt tgataaaaaa tgtttttatt ttagttttga ttatgctgaa cccgaatctg 480

acattacaat ttgcaaattc aaaatggcgg attttttcct aaaaatcctt aaaaagtagt 540

tgtaaaatcc gatttttttt tataaaatgt gtttgtttgc atattatatg tggatatttt 600

tgagaggttg ctgaac 616

<210>495

<211>802

<212>DNA

<213>Diabrotica virgifera

<400>495

catatttctc ttttttgtta gtttcgtatt atcttttttt acaattatca aaaataagta 60

atacaacagg ttttaagaca tcaatatgac actaaaagtg gttttatatt ttgggatgta 120

tctaattgca gagatatctg gacatgggat gatgttaaat cccccaggaa gaagttcttt 180

ctggaggttt aacgacactg cacctcgaaa ttttgacgat aatcaagtgt tctgtggtgg 240

agcttatgtc caaaatgaac tgaatgatgg caagtgtggt gtatgtggag atccttatac 300

agatcctcat ccccaaagaa acgaaaatac cggattttat ggtataggaa ttgtcgtcaa 360

gacctatgaa ccaggctcag ttattgatgt taaaatcatg ctaactgcta atcatctagg 420

aaatttcaca tacagtctat gtgaactcga agattttgac gctccagaac ctgaaaattg 480

ttttgaagat ttgttactgg aggatggttc agctaactat aatgtagagt gggaagatga 540

agtagtcttc aataaggttc gactgccaaa tttccaatgc gagagatgtg ttcttagatg 600

gacttataaa gcaggaaaca actgggggct atgtgaggat ggaaaatgga gaagaggatg 660

tggacctcaa gaacatttca tatcatgcgc agatattgca ctattataat tataaagaca 720

tttaacacta ttcagtaatt ttatagatct tatcctggat aagcgcaccc gcgtaccaaa 780

gaaggttgat tgatggcaag ct 802

<210>496

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>496

ctttctggag gtttaacgac actgcac 27

<210>497

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>497

gcttgccatc aatcaacctt ctttgg 26

<210>498

<211>625

<212>DNA

<213>Diabrotica virgifera

<400>498

ctttctggag gtttaacgac actgcacctc gaaattttga cgataatcaa gtgttctgtg 60

gtggagctta tgtccaaaat gaactgaatg atggcaagtg tggtgtatgt ggagatcctt 120

atacagatcc tcatccccaa agaaacgaaa ataccggatt ttatggtata ggaattgtcg 180

tcaagaccta tgaaccaggc tcagttattg atgttaaaat catgctaact gctaatcatc 240

taggaaattt cacatacagt ctatgtgaac tcgaagattt tgacgctcca gaacctgaaa 300

attgttttga agatttgcta ctggaggatg gttcagctaa ctataatgta gagtgggaag 360

atgaagtagt cttcaataag gttcgactgc caaatttcca atgcgagaga tgtgttctta 420

gatggactta taaagcagga aacaactggg ggctatgtga ggatggaaaa tggagaagag 480

gatgtggacc tcaagaacat ttcatatcat gcgcagatat tgcactatta taattataaa 540

aacatttaac actattcagt aattttatag atcttatcct ggataagcgt acccgcgtac 600

caaagaaggt tgattgatgg caagc 625

<210>499

<211>899

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(793)..(880)

＜223〉n=a, c, g or t/u

<400>499

caactaaaag cttcttgggg acatttgttg caaatcatca ggaaaatgca tatcgagagc 60

gagcagatgc aatttccagg agagttacta cgatggaacc tacatctacg actcctccag 120

aactgataat tccttcaatt acgacaactc tacctccaac aaccacaggc ataaggacaa 180

gggtcgaaac gggcgacgaa gctgtaagct tgggtttacc atgcgtgact gacatgcaat 240

gcaaaatggc tgattcatct tcaaaatgca tagaaggaat ttgcgactgt attattcgga 300

caaatggcac acgatcttgt tcggctaaaa atcgtggatg tattcccgga acatttcagt 360

gtcgaagcac cggtacatgc atcagttggt ttttcgtttg cgatggtaga aaagactgca 420

gcgatggatc agatgaagaa tgcttaccta acaaatgtcc ccaagaagct tttagttgca 480

aatcatcagg aaaatgcata tcgagagcga gcagatgcga tggatccaag gactgtgcct 540

tgggagagga cgaaatgaac tgtcaggcta tcggaagaaa aggttgtcca ccagatactt 600

ttcagtgcag agatgggaaa tgtctacccg agtacgaatt ttgtaatgct ataattggtt 660

gtagtgatgg cagtgacgaa ccacctcaca tttgcaaagg acgagcgaga aggagaagaa 720

cagaatattg tcctttacga tgtggaaatg gcagatgccg atctagcgca attgcttgtt 780

ctggaagaga tgngtgcggt gatggcacag atgaaagtca atgttcagtt tgtagatgtc 840

ccgtgattaa gagcgcactt atangtcgtt aatttttcgn ttgacttgta catgccaaa 899

<210>500

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>500

gcaatttcca ggagagttac tacgatgg 28

<210>501

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>501

ggtggttcgt cactgccatc actac 25

<210>502

<211>617

<212>DNA

<213>Diabrotica virgifera

<400>502

gcaatttcca ggagagttac tacgatggaa cctacatcta cgactcctcc agaactgata 60

attccttcaa ttacgacaac tctacctcca acaaccacag gcataaggac aagggtcgaa 120

acgggcgacg aagctgtaag cttgggttta ccatgcgtga ctgacatgca atgcaaaatg 180

gctgattcat cttcaaaatg catagaagga atttgcgact gtattattcg gacaaatggc 240

acacgatctt gttcggctaa aaatcgtgga tgtattcccg gaacatttca gtgtcgaagc 300

accggtacat gcatcagttg gtttttcgtt tgcgatggta gaaaagactg cagcgatgga 360

tcagatgaag aatgcttacc taacaaatgt ccccaagaag cttttagttg caaatcatca 420

ggaaaatgca tatcgagagc gagcagatgc gatggatcca aggactgtgc cttgggagag 480

gacgaaatga actgtcaggc tatcggaaga aaaggttgtc caccagatac ttttcagtgc 540

agagatggga aatgtctacc cgagtacgaa ttttgtaatg ctataattgg ttgtagtgat 600

ggcagtgacg aaccacc 617

<210>503

<211>1103

<212>DNA

<213>Diabrotica virgifera

<400>503

gacatgttgt tacaaaacat tttggagaga agatcctgat gatgatgttg cagaccataa 60

agtaagatac agtgaagatt gtcaagcatt tgttaatacg actgaagtaa aagaagagtt 120

tataaaagtt acatgcagct acaacaatcg tgatatttat actgatttat ttgcttttgt 180

ccctgtaaaa aacctatcgg aaaatgtgaa aaacgatctg atccaagacg agtcctcttt 240

aaatatttta gtgttgggct tagatgctat ttcaagatta aattttcata gacaaatgcc 300

aaagacatta agttatttag aacaaattgg agccgtagaa atgatgggat ataataaggt 360

tggtgacaat acatttccca atctaattcc agcattaaca ggaaaaactg taaaggaaat 420

cgagaacgat tgctggccaa ctaaaacaca ccattttgat aaatgtccgt tcatatggaa 480

gagctacaaa caaaaaggat atgtaacagc tttcgacgaa gactcttctt gggtgggaac 540

atttaatcat ataaaaaacg gtttcaaaaa acaaccaacg gactatgctt tcaactattt 600

tgattttgag gctataagat tactgggaaa tactgggttg gaaactgttg aaatttgtca 660

aggagcaagg tgggtatata aagttcattt gaactactta tccaatttcg ttcgaacgat 720

ggaagataac ttactaaagt attttggctt tttctggcag aacagtatat ctcatgatga 780

tctaaattta cctagaatag gtgatgatca ttactactct atgtttaaat attttgggaa 840

aaatggttat ttcaatatta catttctctt cgttttgagc gaacattgga ataaaaaact 900

ggtaaaaata agaaagacat tcccagggca tggtgggaaa aaaagattac cgttaccttt 960

ttgtatattt ttcccaaatt ggtaataaaa aaacgatacc catcccggaa aattaaaaaa 1020

tttaaaaaaa aaaatttttt ttggcgttta acaacacccc tttttgtatt ttttattgga 1080

aaattttttt tttcttttgg ttt 1103

<210>504

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>504

ggagagaaga tcctgatgat gatgttgc 28

<210>505

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>505

cgaacgaaat tggataagta gttcaaatga 30

<210>506

<211>691

<212>DNA

<213>Diabrotica virgifera

<400>506

ggagagaaga tcctgatgat gatgttgcag accataaagt aagatacagt gaagattgtc 60

aagcatttgt taatacgact gaagtaaaag aagagtttat aaaagttaca tgcagctaca 120

acaatcgtga tatttatact gatttatttg cttttgtccc tgtaaaaaac ctatcggaaa 180

atgtgaaaaa cgatctgatc caagacgagt cctctttaaa tattttagtg ttgggcttag 240

atgctatttc aagattaaat tttcatagac aaatgccaaa gacattaagt tatttagaac 300

aaattggagc cgtagaaatg atgggatata ataaggttgg tgacaataca tttcccaatc 360

taattccagc attaacagga aaaactgtaa aggaaatcga gaacgattgc tggccaacta 420

aaacacacca ttttgataaa tgtccgttca tatggaagag ctacaaacaa aaaggatatg 480

taacagcttt cgacgaagac tcttcttggg tgggaacatt taatcatata aaaaacggtt 540

tcaaaaaaca accaacggac tatgctttca actattttga ttttgaggct ataagattac 600

tgggaaatac tgggttggaa actgttgaaa tttgtcaagg agcaaggtgg gtatataaag 660

ttcatttgaa ctacttatcc aatttcgttc g 691

<210>507

<211>813

<212>DNA

<213>Diabrotica virgifera

<400>507

tgaagtgaaa gattgctaag gtgaaattga atcggttacg ggggactgta caattgtcgg 60

ttttagtgtt aaacattttt gagaaaaaat ggccagtttt tgggccgttt ttgtttttgg 120

agttgcattc acacttacag agcactgcca cggagctcag tacttcggaa aactcatagg 180

aaaactttcg gaattacacc acggcgtctc cggagaagtt tacgcagtag atgcgaggac 240

tttgtacatc aaggatttca cttatgacgg acaaggaccc gccgcttatt tttacgccag 300

cgtccacaaa gcggctgaca aacacggttt ccggttgaga gatgagacgg gaagcggaga 360

agtaatcagg aggtacagga aggagggaat cactctcacg ctgccagaag gaaagaccat 420

acacagcatc aagatatttt atctttggtg cgaggaattt gacgttaact ttggtgatgt 480

taaagttcct cgtggttttg actatccacg accacaaaaa attgaatctc tcaaaggtgt 540

ccacgcaatt tcgtcagaca acatcgtcat cgtggatgcc cagacgctgt tgattccaaa 600

cttgtcttat gatggcgaag caccagatgc caaattctcg ggttggtcgt ggcagcaaac 660

ctactccaca aggtatccga gtccctgacg agaatggcaa agaagtgccc cttagaagat 720

atgacagaaa gacagtggta cttacacttc caggcgattt gacagttttt gacattggac 780

attttggaat ctggtgcgag atgtttactg ttg 813

<210>508

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>508

tgctaaggtg aaattgaatc ggttacg 27

<210>509

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>509

caacagtaaa catctcgcac cagattc 27

<210>510

<211>799

<212>DNA

<213>Diabrotica virgifera

<400>510

tgctaaggtg aaattgaatc ggttacgggg gactgtacaa ttgtcggttt tagtgttaaa 60

catttttgag aaaaaatggc cagtttttgg gccgtttttg tttttggagt tgcattcaca 120

cttacagagc actgccacgg agctcagtac ttcggaaaac tcataggaaa actttcggaa 180

ttacaccacg gcgtctccgg agaagtttac gcagtagatg cgaggacttt gtacatcaag 240

gatttcactt atgacggaca aggacccgcc gcctattttt acgccagcgt ccacaaagcg 300

gctgacaaac acggtttccg gttgagagat gagacgggaa gcggagaagt aatcaggagg 360

tacaggaagg agggaatcac tctcacgctg ccagaaggaa agaccataca cagcatcaag 420

atattttatc tttggtgcga ggaatttgac gttaactttg gtgatgttaa agttcctcgt 480

ggttttgact atccacgacc acaaaaaatt gaatctctca aaggtgtcca cgcaatttcg 540

tcagacaaca tcgtcatcgt ggatgcccag acgctgttga ttccaaactt gtcttatgat 600

ggcgaagcac cagatgccaa attctgggtt ggtcgtggca gcaaacctac tccacaaggt 660

atccgagtcc ctgacgagaa tggcaaagaa gtgcccctta gaagatatga cagaaagaca 720

gtggtactta cacttccagg cgatttgaca gtttttgaca ttggacattt tggaatctgg 780

tgcgagatgt ttactgttg 799

<210>511

<211>1550

<212>DNA

<213>Diabrotica virgifera

<400>511

aaagactcgt tttaagtagg tgtgtaaaaa tttttatgaa ataatataaa aaaaacgtgt 60

cgattcctgt gtatattaaa aattggggtt agattgtatt aacttattat ctgcacacaa 120

tttctgagga agttaacaaa taatatttcc tgagccgaaa ctgaaagaat acagcatgca 180

tcacagtaat taaaaaaaat ctattatctg tatctgttat tataatagtc tcccgtttta 240

taccgctaaa gcggctttgg gattatagca gggtagtctg ctatatctag tgcctacggt 300

atacaagaaa ggtaacaggg tcagtgctac gcttcaaccg cctattatta cccctggttt 360

tacccaaagt actaatttta ttcaggctga gtcgacctgg ggcctataga catttttaaa 420

aatgtctaga tgttctcgcc ggcgcgggtt tcgaaccccg gcctaccagc atgcgagtca 480

gatatcctac agcctgagct acggcggccc ttattaaata gcaaaatgtt aacggacaaa 540

ttaacaataa ttttatatat cttaaggtct aggttatagc gtatttttaa tcgactaata 600

aattatttaa catatccatt atttgaatca aaaataaaga agccattcta aaatttacat 660

aaagacacat aataatatat taactaactt cttgaaaata cgccactgaa tgaatgcaga 720

aaatctgatc gtttcgccgg acgggcctta ggtaactctt agcgaaaatc gccattttgc 780

gcacgttatt cgcactatac gttatacatt gtaacgctag acatacccag actagtagat 840

acttagccca aacctaataa tgttaaaaat caatcgttat ctaccaccaa ttttatcgga 900

tcaggaatat agtacaaatt taataactaa aggcagctat aaagcaaata tagattgtct 960

tgaaatttga tttaaatgaa attttgaaac cgcaaaaaga cattcatgga atattgaaaa 1020

ttgtaaaaga tttatgaaat ttctaatcat ttctttttcc taacaagtag aatccagttc 1080

aataggacta gctttgaaat gcaaaactat attacgcaat aatgacatgt aatcctaaaa 1140

attttaatat taaatgacta atgaacatta caaatttatt aatacattga tagtttcgat 1200

gtgactcgat aaatcaaacg tattttggta gcgtaaaaac caaggaaaca gtattgaaga 1260

cattttaaat ttttccacat atattgactt ttttcataga aatcgaatta aaagtggttt 1320

tatttcactt taccttctaa ttgtttatta ataactttaa atagttaatt aacttattac 1380

ttctaagaat tttaacaaac aaaagatttg gttatttttt tataacttta ccatgcttaa 1440

taattttaca cttaatttaa tagcaccaaa atttacatgt ttataattta tataaacata 1500

aatcaagttt tgcataatgc agaacgaaat tttggaattt tagtttaatc 1550

<210>512

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>512

cagggtagtc tgctatatct agtgcctacg g 31

<210>513

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>513

gctagtccta ttgaactgga ttctacttgt tagg 34

<210>514

<211>824

<212>DNA

<213>Diabrotica virgifera

<400>514

cagggtagtc tgctatatct agtgcttacg gtatacaaga aaggtaacag ggtcagtgct 60

acgcttcaac cgcctattat tacccctggt tttacccaaa gtactaattt tattcaggct 120

gagtcgacct ggggcctata gacattttta aaaatgtcta gatgttctcg ccggcgcggg 180

tttcgaaccc cggcctacca gcatgcgagt cagatatcct acagcctgag ctacggcggc 240

ccttattaaa tagcaaaatg ttaacggaca aattaacaat aattttatat atcttaaggt 300

ctaggttata gcgtattttt aatcgactaa taaattattt aacatatcca ttatttgaat 360

caaaaataaa gaagccattc taaaatttac ataaagacac ataataatat attaactaac 420

ttcttgaaaa tacgccactg aatgaatgca gaaaatctga tcgtttcgcc ggacgggcct 480

taggtaactc ttagcgaaaa tcgccatttt gcgcacgtta ttcgcagtat acgttataca 540

ttgtaacgct agacataccc agactagtag atacttagcc caaacctaat aatgttaaaa 600

atcaatcttt atctaccacc aattttatcg gatcaggaat atagtacaaa tttaataact 660

aaaggcagct ataaagcaaa tatagattgt cttgaaattt gatttaaatg aaattttgaa 720

acagcaaaaa gacattcatg gaatattgaa aattgtaaaa gatttatgaa atttctaatc 780

atttcttttt tcctaacaag tagaatccag ttcaatggat aagg 824

<210>515

<211>1452

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(1400)..(1420)

＜223〉n=a, c, g or t/u

<400>515

aataaataaa ccaatttaat atattagaac atttttaata atacttttat tttaaatcaa 60

tgtattcata aataatcttg tcactggcat actagcaaaa accatgtaac ttaaaactca 120

tttcataaag tttataagtt atgctgatga gttttgttat cagaagatta cgtacctatc 180

agcttttaat cattcgcata ctttggtgtg tttttatcaa ataaggctaa taagaaaatt 240

catgataaaa cattcggaaa tgtttaaata caacacgcaa ttaaaaaaaa gcagcataaa 300

atttagacga attagtacaa agtgtaccta cttaaaattt acctttttga gtaatttttt 360

atgctgagaa tttaaaaaaa aatccgataa ttttgtctaa aagtacagaa aatatttttt 420

ttaggttata ctttaataag ttacatggtt ttgaatagca tttgaaatgt tttctaagta 480

ctagctagga tttttttatt tattatctaa tcaatcacat tttacactaa tattctcaaa 540

atcacattta gattttaata aaaaaattct ccatttgtaa aatttaaatt gttctccata 600

gtaaatgcaa atttataaag taaaaaaaca ctaaactatc tatcattaat ataggtccca 660

tagataataa taaatagaaa gaatattata atagttattt tcctaagaag tgcagatagt 720

cattattttc cgcacgcgac tgcagtttgc cgaacgacgc gaagcgggag ttcggcaacc 780

agtcgagtgc ggaaaagaga ctttctgtaa gagttaggaa caatattttt tacgagtctt 840

taaaaaatta ccaaatctta atcaattaat ttaattaata tgaaaataca tacacaaatt 900

aattctttga gaaggttgtc aaaaccaaac tttcaatata attagttagc atgacgacga 960

tcttggtttc catgacgatg attcaaaacg actgttattg tctaccgatt tgactttcga 1020

atattatgtc aaaataatta tatttcatcg aattgtcgcg ttaatttcag gaacacaata 1080

caagatatat ttgaaataaa ttagtaaata atatctaaat attagtttat tgcatgtatt 1140

ataattactt taaggccata ttaacatatc taaattaaca cgcgtgcgga aaagtaacac 1200

gcgtgcggaa tagtgaaact ttctaaacta aaatgggtgc gcgaaagtag acatttttgc 1260

acgctcgtag aaaaatgatt tattgacgtt tcgacttcca cttcggaggt cgttatctaa 1320

atacaaaata ctcgacaaac tgaaaagtcg ccgaattgcg tcccagtggg gatttcaatg 1380

atctttgaaa tttcccttan atagatggac tagctggttn tcgcttcaga gatgtacact 1440

ctgctctgct ga 1452

<210>516

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>516

tgctgatgag ttttgttatc agaagattac g 31

<210>517

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>517

ggaaaccaag atcgtcgtca tgctaac 27

<210>518

<211>828

<212>DNA

<213>Diabrotica virgifera

<400>518

tgctgatgag ttttgttatc agaagattac gtacctatca gcttttaatc attcgcatac 60

tttggtgtgt ttttatcaaa taaggctaat aagaaaattc atgataaaac attcggaaat 120

gtttaaatac aacacgcaat taaaaaaaag cagcataaaa tttagacgaa ttagtgcaaa 180

gtgtacctac ttaaaattta cctttttgag taatttttta tgctgagaat ttaaaaaaaa 240

atccgataat tttgtctaaa agtacagaaa atattttttt taggttatac tttaataagt 300

tacatggttt tgaatagcat ttgaaatgtt ttctaagtac tagctaggat ttttttattt 360

attatctaat caatcacatt ttacactaat attctcaaaa tcacatttag attttaataa 420

aaaattctcc atttgtaaaa tttaaattgt tctccatagt aaatgcaaat ttataaagta 480

aaaaaacact aaactatcta tcattaatat aggtcccata gataataata aatagaaaga 540

atattataat agttattttc ttaacaagtg cagaaagtca ttcttttccg cacgcgactg 600

cagtttgccg aacgacgcga agcgggagtt cggcaagcag tcgagtgcgg aaaagagact 660

ttctgcaagc gttaggaaca atattttttc tacgagtctt taaaaaatga ccaaatctta 720

atcaattaat ttaattaata tgaaaataca tacacaaatt aattctttga caaggttgtc 780

aaaattaaac ttcagcataa ttggttagca ttacgacgat cttggtcc 828

<210>519

<211>1380

<212>DNA

<213>Diabrotica virgifera

<400>519

gagtttcttc ttgcgtaaat aatttggtat ttttttatgt ttattgattc cgtttatccc 60

ttccattttt ttcgattatt attcctgtgg aatagattcg cgttttctag tgtcttctcg 120

tactaatatc tcctctatag aaactaaata atataaaaaa tatataagac aaatataaaa 180

tctgttacac aactgtcatt agagattgaa ataaagctta agatttttta acttgcctac 240

aatagagttg ataacaggtt ttgtttcaca tctctatact tatgcctgta aactgtagaa 300

tctgttccga atgtcagcat taagttacac attaactgcc gatatctgtt ctttcttaaa 360

taattgccag aaaatttcgt ctaatcttca acgccataaa tgcaatgtaa aaattaatct 420

tagtatactt gtgagtgcag aaggtgtgct attgcctttt taaacacatg gcatacttac 480

attatattat cgttaaatgt attcctatcc agacctcgtt ccaacatatt gcggctagga 540

gcagaagaat aagacagctg acaagctaac ctactgacag ttgacattga cattattatt 600

atgaatgaca caaaatgtat ttaagttcag tcatcatttt tatataaaat tcacatctta 660

ttgctaaata ttattttcca gttatttgat tataaagatc agtgcaacgt agtagcctat 720

ataaatattc taaatctact cacagccaag aagaaacagg ctaaacccgc taccttattc 780

ggaaagttgg ttacgtgatg attatgaatt tgactctttt tattagctgt gaatatgtat 840

ttagtaataa aatatacttt atatcaaaac ggtaactttg cttcacaata gggctattta 900

ttgcatcatt gccaccattt ttctcgcaca cttgacatat gacatacaat atgcgtcata 960

cgtctttgat atataattgg tatataccaa tgacgtatta cgtatatatt gtacgtcata 1020

tgtcaatagc catctggaac atgcgcatat cgtaatattt cactattatc tcaactgtca 1080

cttacctctg caaacaggta ttcaaatgca ggtctggaaa ctggctttgg ttaagattac 1140

gcctaacggt agcataatgt aggtgtgtta taactaaaaa taaaaagaat cctacactta 1200

cggacaccta cgatatgtgt taagcactgt taaaatgcta tagttctatt ataaggaaca 1260

aaaaagatga atatgcaaga aattaataat attgttataa attaccagaa gataagtgtt 1320

aactgtactt catagagtct agattatcct aataatactt agataataat cagttttaag 1380

<210>520

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>520

cttgcctaca atagagttga taacaggttt tg 32

<210>521

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>521

gcttaacaca tatcgtaggt gtccgtaagt g 31

<210>522

<211>993

<212>DNA

<213>Diabrotica virgifera

<400>522

cttgcctaca atagagttga taacaggttt tgtttcacat ctctatactt atgcctgtaa 60

actgtagaat ctgttccgaa tgtcagcatt aagttacaca ttaactgccg atatctgttc 120

tttcttaaat aattgccaga aaatttcgtc taatcttcaa cgccataaat gcaatgtaaa 180

aattaatctt agtatacttg tgagtgcaga aggtgtgcta ttgccttttt aaacacatgg 240

catacttaca ttatattatc gttaaatgta ttcctatcca gacctcgttc caacatattg 300

cggctaggag cagaagaata agacagctga caagctaacc tactgacagt tgacattgac 360

attattatta tgaatgacac aaaatgtatt taagttcagt catcattttt atataaaatt 420

cacatcttat tgctaaatat tattttccag ttatttgatt ataaagatca gtgcaacgta 480

gtagcctata taaatattct aaatctactc acagccaaga agaaacaggc taaacccgct 540

accttattcg gaaagttggt cacgtgatga ttatgaattt gactcttttt attagctgtg 600

aatatgtatt tagtaataaa atatacttta tatcaaaacg gtaactttgc ttcacaatag 660

ggctatttat tgcatcattg ccaccatttt tctcgcacac ttgacatatg acgtgcaata 720

tgcgtcatac gtctttgata tataattggt atataccaat gacgtattac gtatatattg 780

tacgtcatat gtcaatagcc atctggaaca tgcgcatatc gtaatatttc actattatct 840

caactgtcac ttacctctgc aaacaggtat tcaaatgcag gtctggaaac tggctttggt 900

taggattacg cctaacggtg gcataatgta ggtgtgttat aactaaaaat aaaaagaatc 960

ctacacttac ggacacctac gatatgtgta agc 993

<210>523

<211>1253

<212>DNA

<213>Diabrotica virgifera

<400>523

cgtccgtctt catcttctaa tctagaacaa tatgttacaa gcctgttcaa tgcagaagga 60

tggagaaagt attctaactt attaggccta gccctggatt ggttaggatc tttgtctgta 120

tcgtcaacca atacagatca atttgaatat aaactttcaa atttgataca taaattaaga 180

gtcattacct atgcttgtaa ttaggaatta ttgtactctc aaaataatta atattaaatc 240

cagtagtgat tgatcatatt ggaaggtaaa gtacaggaca aaagattagt aagaagacgc 300

tcgaactcgt ggctgaagga cctgacaaaa cggtttgacc gctcatccgc agaaattttt 360

cttgcagcag ttttcaaagc tacagttgcc atttggatcg ccaatctttg aaaggagacg 420

gcgcaataag aagaaatgat tgatataacc tcaacaaaca gtgacttact ttactttcat 480

tatcaacaaa acaaatgttt tgtagagaaa gcaaactagt gctgctgcta gtggtgaatt 540

aatatactaa tgattaaagc cactttcagt ctgaatagta ctatgttaag ggttccattt 600

acgttgagat aggtatcgat acatgtatcg cgacaagttg cgatacacgt atcgtgagat 660

ttgtgtctgt ttcacgacag agatgcatgc gacgatagag ttgtagacga cgaagattga 720

gagttgtgat actgatacag cgatactgag acagccaaat acacgatgag atttgtatca 780

cgatactggt acttgcgaca tatatcttaa aattctaaaa tttttaatct ttctacagaa 840

gtatcttata attagaaatt gtttataata taaatgaaaa atttatttaa ctttactcta 900

gattatttaa taaaacaatt ctagtttaat tgtattaata ttattatatt tacaataaaa 960

ccaagtttaa gaatattctc atgtataata aatgaacttc aaaaacgaca gacactatct 1020

tgacacatct tgtaaataat cacatgtgtt aaatttttgt tataaaatat tttatatgta 1080

taatgtttac ttttatttaa tacgtttgtt taattgttgg gacatttgta atactgtagc 1140

aaaaaccgaa aatattattt atttgcgatt gcaatgacat gatgttattt catttaaatg 1200

taagattatg tgtgatcttt agcgtaacat gaaataaagt tttataatat tgg 1253

<210>524

<211>22

<212>DNA

<213>Diabrotica virgifera

<400>524

gacgctcgaa ctcgtggctg aa 22

<210>525

<211>33

<212>DNA

<213>Diabrotica virgifera

<400>525

gctacagtat tacaaatgtc ccaacaatta aac 33

<210>526

<211>845

<212>DNA

<213>Diabrotica virgifera

<400>526

gacgctcgaa ctcgtggctg aaggacctga caaaacggtt tgaccgctca tccgcagaaa 60

tttttcttgc agcagttttc aaagctacag ttgccatttg gatcgccaat ctttgaaagg 120

agacggcgca ataagaagaa atgattgata taacctcaac aaacagtgac ttactttact 180

ttcattatca acaaaacaaa tgttttgtag agaaagcaaa ctagtgctgc tgctagtggt 240

gaattaatat actaatgatt aaagccactt ccagtctgaa tagtactatg ttaagggttc 300

catttacgtt gagataggta tcgatacatg tatcgcgaca agttgcgata cacgtatcgt 360

gagatttgtg tctgtttcac gacagagatg catgcgacga tagagttgta gacgacgaag 420

attgagagtt gtgatactga tacagcgata ctgagacagc caaatacacg atgagatttg 480

tatcacgata ctggtacttg cgacatatat cttaaaattc taaaattttt aatctttcta 540

cagaagtatc ttataattag aaattgttta taatataaat gaaaaattta tttaacttta 600

ctctagatta tttaataaaa caattctagt ttaattgtat taatattatt atatttacaa 660

taaaaccaag tttaagaata ttctcatgta taataaatga acttcaaaaa cgacagacac 720

tatcttgaca catcttgtaa ataatcacat gtgttaaatt tttggtaaaa atattttata 780

tgtataatgt ttacttttat ttaatacgtt tggttaattg gttgggacat ttgtaatact 840

gtagc 845

<210>527

<211>1205

<212>DNA

<213>Diabrotica virgifera

<400>527

attgactttt actgctgtct attactgtag cggagctatt gagctttacc ttactaatca 60

atcaccctgt atacctagat agacttttag tatagaaata taaaaaatat gcaaaatgca 120

tatgttatta acttctgtaa tcctgaattt cctaaaaaat aattaaatgt tgaccttacg 180

aattataccg ttacgaatat gtagttacga actctcgccg ttacgaattg tccgttacca 240

tttgtcgggt tacgaactgt cgcgttacga gatgtcatgg aaccataata acacacgcaa 300

ggataaaaag atacaaggat aagataacag attgtacgtg gccgacatta tcagttatgt 360

ttggagataa aatggttccg gttcgttaga atttaacaga aatattcaag tgttcaaggt 420

tagaaaacta aactgtttga ttaaaaaaaa ttttttttca ttaatttcta agtaaaaata 480

ccattttatg ttcaaagagc cgatattgta gcccccttaa taattatttt tatgtttgtc 540

ccttgtccag tgtgtgttat ctatttgtat tatttttaat taaatgtctg acattccggg 600

atttgctgga taacttcctt ttttgacgga atgccccctg atgatgctat gagagcgaaa 660

ctacttgggc aaaatttaat aaataactgc tcgatatctg cgttttattt gtaaaattca 720

tatattcgag cattgaacta tatattattg aattgtaata tagttaaaag gcaattaaga 780

taaattttta cagagtttat cgtgttcttc tatgattatt aaataattct agtttatttg 840

ttatctcatt actagatcta gtaagagata acccatgaat tttttaaaat gtttatccaa 900

ttctgatata tctgttaagt acataataat gaacctatta aataagtcgc agattctaca 960

tagtaaaaaa aacttgtttc aataaatttg aaagttcaaa acgctttatc cagaaagttt 1020

gtaaatatat caaaaatatt atcagtagta attgcacaag agctctaaaa ttattgaatt 1080

tttcccgagt gacactttga cagttttaat ttcacgagcc gaagtgtcac gagggcaaaa 1140

tactatatta attttagaga tcgagtgcaa tttgttgcga ttatttcatg aataaaactg 1200

ttcaa 1205

<210>528

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>528

gctgtctatt actgtagcgg agctattgag c 31

<210>529

<211>36

<212>DNA

<213>Diabrotica virgifera

<400>529

ctatgtagaa tctgcgactt atttaatagg ttcatt 36

<210>530

<211>947

<212>DNA

<213>Diabrotica virgifera

<400>530

gctgtctatt actgtagcgg agctattgag ctttacctta ctaatcaatc accctgtata 60

cctagataga cttttagtat agaaatataa aaaatatgca aaatgcatat gttattaact 120

tctgtaatcc tgaatttcct aaaaaataat taaatgttga ccttacgaat tataccgtta 180

cgaatatgta gttacgaact ctcgccgtta cgaattgtcc gttaccattt gtcgggttac 240

gaactgtcgc gttacgagat gtcatggaac cataataaca cacgcaagga taaaaagata 300

caaggataag ataacagatt gtacgtggcc gacattatca gttatgtttg gagataaaat 360

ggttccggtt cgttagaatt taacagaaat attcaagtgt tcaaggttag aaaactaaac 420

tgtttgatta aaaaaaattt ttttcattaa tttctaagta aaaataccat tttatgttca 480

aagagccgat attgtagccc ccttaataat tatttttatg tttgtccctt gtccagtgtg 540

tgttatctat ttgtattatt tttaattaaa tgtctgacat tccgggattt gctggataac 600

ttcctttttt gacggaatgc cccctgatga tgctatgaga gcgaaactac ttgggcaaaa 660

tttaataaat aactgctcga tatctgcgtt ttatttgtaa aattcatata ttcgagcatt 720

gaactatata ttattgaatt gtaatatagt taaaaggcaa ttaagataaa tttttacaga 780

gtttatcgtg ttcttctatg attattaaat aattctagtt tatttgttat ctcattacta 840

gatctagtaa gagataaccc atgaattttt taaaatgttt atccaattct gatatatctg 900

ttaagtacat aataatgaac ctattaaata agtcgcagat tctactg 947

<210>531

<211>1194

<212>DNA

<213>Diabrotica virgifera

<400>531

aaaacgtaag tctttctttc ggccacggga acggaacagg ttagtccgta acaacagaga 60

aaaagtttcg ttaaatcact tcgtatcaat cttcggcatc atcttaaaat tttcttcaga 120

atcagtagga aaactcaaag taggttattc tcatcggtta cattcgttct tcggggttta 180

tagaccgagc tcggaattat ttcttcctcg gcctcgcaac ttccatcgag acgtcaaagg 240

cagcgtgtaa actatcaatg agtagaagac agttcataac ggtcttctac ccgacagtta 300

tgacctatgt tgcatagtct tcgatgtctg tcacagttga aaggcacaag aaagaaatag 360

atccacatca cgaccaatta taaagcccaa aaagaattta ccattacaga acaccagccg 420

tgaaggctta cattcctcaa aatcatgtca gtcatatgtg gggatggtga catggttata 480

gtcatggggt tttatggtat tatttctata catggttttg aataatgtaa gtctcgtcat 540

taaatatgta tctgacaata catttgaatt taaatactcg agattaaatt ataagcaatg 600

atcagaagaa atgagatact tagtgccttg gatatgtgtt aaccattgct tcgctttttt 660

aaagacaaat gttcactgca caatgtaaaa cgactagtaa ctagtaaaac aaaaattccc 720

tggtttgttc ttgcagacta ttaaaaagaa gtagttttta atagctgatg gacaaaacag 780

gcacaaatat tgacaaattg cacagtagtg aggctccaaa agccttagta attaggaaca 840

aaatctttaa atagctcttt aataaaatgt tttatactta catgaaaagg ttgcagccac 900

ccatccttgg aatagttgat gaaagagaaa gaatttaatg aaggtgaaaa tgaaacatga 960

tatgaaccag atgagatgtg ataggaatgt ggatttggaa acgaaggact atggatatta 1020

aaaaaataag gtgatggcaa aattcacctt aaaaaggcaa cttcctgaag ggaagttact 1080

taacccacct ccacccagtc cgaatatttc ttgaggagaa atattcggcc tacttactaa 1140

aatatattgg gggatcaaga caaattatga tccctgcatt tggcttaaat cttg 1194

<210>532

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>532

agtccgtaac aacagagaaa aagtttcgt 29

<210>533

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>533

gaggtgggtt aagtaacttc ccttcagg 28

<210>534

<211>1031

<212>DNA

<213>Diabrotica virgifera

<400>534

agtccgtaac aacagagaaa aagtttcgtt aaatatttgt ataattttcg gcatatctta 60

aaatttcttc agatcagtgg aaaactaaag tggttatctc atggttacat tgttcttggg 120

gtttatagac cgagctggaa ttatttcttc ctggcctgca acttccatga gacgtcaaag 180

gcagcgtgga aactatcaat gagtaggaga cagttcataa cggtcttcta cccgacagtt 240

atgacctatg ttgcatagtc ttcgatgtct gtcacagttg aaaggcacaa gaaagaaata 300

gatccacatc acgaccaatt ataaagccca aaaagaattt accattacag aacaccagcc 360

gtgaaggctt acattcctca aaatcatgtc agtcatatgt ggggatggtg acatggttat 420

agtcatgggg ttttatggta ttatttctat acatggtttt gaataatgta agtctcgtca 480

ttaaatatgt atctgacaat acatttgaat ttaaatactc gagattaaat tataagcaat 540

gatcagaaga aatgagatac ttagtgcctt ggatatgtgt taaccattgc ttcgcttttt 600

taaagacaaa tgttcactgc acaatgtaaa acgactagta actagtaaaa caaaaattcc 660

ctggtttgtt cttgcagact attaaaaaga agtagttttt aatagctgat ggacaaaaca 720

ggcacaaata tcgacaaatt gcacagtagt gaggctccaa aagccttagt aattaggaac 780

aaaatcttta aatagctctt taataaaatg ttttatactt acatgaaaag gttgcagcca 840

cccatccttg gaatagttga tgaaagagaa agaatttaat gaaggtgaaa atgaaacatg 900

atatgaacca gatgagatgt gataggaatg tggatttgga aacgaaggac tatggatatt 960

aaaaaataag gtgatggcaa aattcacctt aaaaaggcaa cttcctgaag ggaagttact 1020

taacccacct c 1031

<210>535

<211>1024

<212>DNA

<213>Diabrotica virgifera

<400>535

cggacgcgtg ggctttttgg acaaagtatt tggtggtttc ttagtcctcg aaatctctag 60

atttggattg atttaattta cattattgtc attgtcaaaa agtacatagg gcattgcaac 120

atgctgtcaa atctaaatca atatgggggc tggtaggcaa acctaaatgt cattctatca 180

gttttcaatg tgttttagat cattttagtt gcgtttcttt gtaattttgt tttgtacaat 240

gattaattaa cattttcact ggaagaaata atgtaaaaag ataatatgcc tgatttatgt 300

tgtgttttga agtgtataat gcgaagtcat catgataaaa agttcagttt tattggttgc 360

catcagtgct acattttaag cataaacaac taaataaagt tatcatctta gggacaaaaa 420

caatggttaa atgctataaa acgtcctata aaaatgctaa ataactatat ccgtgttact 480

caagcttgga aaactgtctc caccgtaggc taacaccgac cacttgagga aaatggacct 540

atattgtaca aagactgtgc caattacaga gatccttcat tatggatttc ttcaagattt 600

gtaaatgtcc tctgtaataa ctattattca ccataggtca gtaattattg atttctaacc 660

ttcaaaatag tcgagaagtg acctattatt tactttacta tctccactgt ctaggaaagt 720

acttatcggc tatcatatgt cactttcctt gccgtcaaat gtcacttgtc ataaatgtca 780

aaataattta tcaaattata ataaaagtta atgtagatat tattatttga aacttaatta 840

ttaatgatat attatgacta aaatagtata catatgtata ctcgggagaa tgtatttcag 900

cctcgcgtct gtagcttact ttggtaattc ctgaacaagc taatttgaca atcaattctc 960

ctgggcaata tgtaatatac tatgactctt agtgatatcg acttagtcgt aaaccactgg 1020

ttct 1024

<210>536

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>536

gacaaagtat ttggtggttt cttagtcctc g 31

<210>537

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>537

gttcaggaat taccaaagta agctacagac g 31

<210>538

<211>917

<212>DNA

<213>Diabrotica virgifera

<400>538

gacaaagtat ttggtggttt cttagtcctc gaaatctcta gatttggatt gatttaattt 60

acattattgt cattgtcaaa aagtacatag ggcattgcaa catgctgtca aatctaaatc 120

aatatggggg ctggtaggca aacctaaatg tcattctatc agttttcaat gtgttttaga 180

tcattttagt tgcgtttctt tgtaattttg ttttgtacaa tgattaatta acattttcac 240

tggaagaaat aatgtaaaaa gataatatgc ctgatttatg ttgtgttttg aagtgtataa 300

tgcgaagtca tcatgataaa aagttcagtt ttattggttg ccatcagtgc tacattttaa 360

gcataaacaa ctaaataagg ttatcatctt agggacaaaa acaatggtta aatgctataa 420

aacgtcctat aaaaatgcta aataactata tccgtgttac ttaagcttgg aaaactgtct 480

ccaccgtagg ctaacaccga ccactggagg aaaatggacc tatattgtac aaagactgtg 540

ccaattacag agatccttca ttatggattt cttcaagatt tgtaaatgtc ctctgtaata 600

actattattc accataggtc agtaattatt gatttctaac cttcaaaata gtcgagaagt 660

gacctattat ttactttact atctccactg tctaggaaag tacttatcgg ctatcatatg 720

tcactttcct tgccgtcaaa tgtcacttgt cataaatgtc aaaataattt atcaaattat 780

aataaaagtt aatgtagata ttattatttg aaacttaatt attaatgata tattatgact 840

aaaatagtat acatatgtat actcgggaga atgtatttca gcctcgcgtc tgtagcttac 900

tttggtaatt cctgaac 917

<210>539

<211>1017

<212>DNA

<213>Diabrotica virgifera

<400>539

tataaaatct ggcaactgtg cagtgtagta caaccctcgc cactcacaga cagcggacgg 60

gatgatcgca tcgcatcgtg ccaaaagctt agatggctca gctgaaaaga gaaagatttt 120

acgggcattc tatgtaagtg acagagatag agctatattg cacttttaat acacgtttaa 180

atgaaactat tcgtgcctgg cccgacaagg caggaagatc taatattttt tactatgctc 240

atcatagtga atagtggatc gtagatcaga tccaaaaagc ttctcgtaca gggtagtacc 300

tacattatct tttcgagttt ttttacgcgc tcgctcgctc gtttttgtat ttataattat 360

tacattttta atgattaaac tttttattct aaactattat atttttaagt aatttaaaat 420

ttttaataaa acccgagtta aattcagctt atcgtgatag tctaattaaa cacaatgaac 480

aagaagtgga ttaagaatcg atatgttttg aatcaaatta aaaattatat ctaagtatat 540

ggttttgtgg agctttcgag ttagttttga gaaatcacga cgaaaaggaa aattcagaaa 600

atataggcat ctttatggaa ctgttcaatg ttagccgaat tagacaacga cttcggttaa 660

tattcaaagt tccaaatctt taaaagcatg tacctcaaaa caacacaaaa tgaactcctt 720

cagtgcatct tagatgttta tcacgaggaa ataaggaaag aaattgaaaa ttatctttgt 780

gttgcagtga ttgccgatga aacgacagac gtcgcgtcgc tggtgaattt cagttagtta 840

tagttttccg atatttgtgt aaagggcgat aagttgagag attttggaga ttttacttgt 900

tggacggaca tgatgtcaaa tcaatcactg aatgcatttt aaatgtttaa atttcttgac 960

accccagaaa tggtatatac ctacataatg taaaaatatc cgtacctatt tttttta 1017

<210>540

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>540

caactgtgca gtgtagtaca accctcg 27

<210>541

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>541

cagtgattga tttgacatca tgtccgt 27

<210>542

<211>922

<212>DNA

<213>Diabrotica virgifera

<400>542

caactgtgca gtgtagtaca accctcgcca ctcacagaca gcggacggga tgatcgcatc 60

gcatcgtgcc aaaagcttag atggctcagc tgaaaagaga aagattttac gggcattcta 120

tgtaagtgac agagatagag ctatattgca cttttaatac acgtttaaat gaaactattc 180

gtgcctggct cgacaaggca ggaagatcta atatttttta ctatgctcat catagtgaat 240

agtggatcgc agatcagatc caaaaagctt ctcgtacagg gtagtaccta cattatcttt 300

tcgagttttt ttacgcgctc gctcgctcgt ttttgtattt ataattatta catttttaat 360

gattaaactt tttattctaa actattatat ttttaagtaa tttaaaattt tttaattttt 420

aataaaaccc gagttaaatt cagcttatcg tgatagtcta attaaacaca atgaacaaga 480

agtggattaa gaatcgatat gttttgaatc aaattaaaaa ttatatctat atggttttgt 540

gaagctttcg agttagtttt gagaaatcac gacgaaaagg aaaattcaga aaatagaggc 600

atctttatgg aactgttcaa tgttagccga attagacaac gacttcggtt catattcaaa 660

gttccaaatc tttaaaagca tgtacctcaa aacaacacaa aatgaactcc ttcagtgcat 720

cttaaatgtt tatcacgagg aaataaggaa agaaattgaa aattatcttt gtgttgcagt 780

gattgccgat gaaacgacag acgtcgcgtc gctggtgaat ttcagttagt tatagttttc 840

cgatatttgt gtaaagggcg ataagttgag agattttgga gattttactt gttggacgga 900

catgatgtca aatcaatcac tg 922

<210>543

<211>999

<212>DNA

<213>Diabrotica virgifera

<400>543

catacatacc acagcgaagc gtttcctgaa cctcgatgtg cgaggtttgt gtttaattta 60

tcctttggcg ctgtagaagt ttattacacg catgcattaa gaaaagtacc tccaattaag 120

attaagaatt taagcaagtt ttaagaattt aggcaagtta tctccacagc actaaagaaa 180

ttaagaaaaa taaagtcaga gtgtttgtct caattgtatc aagtttagaa gagcgaggat 240

aatggatctc gtcatgtcct tttataccca ctcaagactg tacaaaaaga caagcactct 300

agacaatcaa ttcaagacaa gtactcatct taacgcagtc aagaagtttg tttgtagata 360

taagtacatt ttttttgtta agtttccatt ctaaatggtg ggccagtttt gtcaagttta 420

gaaacaagat ttgggcaaga ggttcgtaag agggaagaat agaagaaagc aactaataca 480

gtttgtagca ttttagaaaa aaaaattaga agataacttg aaaacggaga ctaaacttta 540

atgaactatc aagtttatta tcattaacac cttttattct cggtaacaag tatgtaaaag 600

caaatattat ttatggagta gaaaaaataa tgtttgttcc acttctgtca agaagacaga 660

atgaaattca agctggttaa attaaataaa agattctctt gtctaatgaa ttgcattgaa 720

gatatcttca cctgattgtc acatgcctgt cgaaaatatc tagctatatc agaataataa 780

ggactttcat aaattacgaa aaattaacga agcaaagaaa actgtttgaa aatcaattaa 840

gaaacattcg gaaaggccaa tattttgttt ttttgtgttt acataaaatc taaagatata 900

taaatattac atttatatgt acggatttaa gaaaatgaaa cctggtattc aatatgtaat 960

tccgaaaaat atcacataga aataaaattt attatacat 999

<210>544

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>544

catacatacc acagcgaagc gtttcc 26

<210>545

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>545

cgacaggcat gtgacaatca ggtg 24

<210>546

<211>508

<212>DNA

<213>Diabrotica virgifera

<400>546

catacatacc acagcgaagc gtttcctgaa cctcgatgtg cgaggtttgt gtttaattta 60

tcctttggcg ctgtagaagt ttattacacg catgcattaa gaaaagtacc tccaattaag 120

attaagaatt taagcaagtt ttaagaattt agggccagtt ttgtcaagtt tagaaacaag 180

atttgggcaa gaggttcgta agagggaaga atagaagaaa gcaactaata cagtttgtag 240

cattttagaa aaaaaaatta gaagataact tgaaaacgga gactaaactt taatggaaga 300

actatcaagt ttattatcat taacaccttt tattctcggt aacaagtatg taaaagcaaa 360

tattatttat ggagtagaaa aaataatgtt tgttccactt ctgtcaagaa gacagaatga 420

aattcaagcc ggttaaatta aataaaagat tctcttgtct aatgaattgc attgaagata 480

tcttcacctg attgtcacat gcctgtcg 508

<210>547

<211>969

<212>DNA

<213>Diabrotica virgifera

<400>547

ttgactacaa atgaaaaaaa actcttatta actactttac tttcagattg gaaaatactg 60

acaaaataca attaaaacat tgtattcttt caaaatggtt accaatctag aggtaaaaaa 120

gtgcataata atagtttatt ttaaaatttg cagtttattt tcaagagatt ttcaaaaaat 180

gattttttct ttactgtaca ctcctgtatt agaagtagaa atcagcgagt gttaattact 240

tcgttctaaa taattatttt tgttgaagat ttaattctta ctgattctat tgtctatctc 300

aaataatatc tgtttaatga agtattatga atagttgtac ttttttagtt aatatttaat 360

aacttcacta tccaatcaac tatataattc aagaacctta atgtgtccct aatatgtatg 420

tccatgaatc aaatgtgatt caaggataga agctattcta aaattaaaat agtattggac 480

atagactcct gattatgtta acattgggtc agtggagtta tcaatatcac taagaaatgg 540

cacatttaga aaaaaactaa aggtaattag ttttcaaatc gtacaattat tgttctcaaa 600

acataaaaca gcattctaat attctttacc tattaatact ctattctgtt ttcaattgtt 660

ttaaatccat aaataaacag ctgtcaaaca atcataacat tctacaacca agaaaccatg 720

aaatgtccat gccacattaa tattgaaaca aattttttgg tttatgttac aacaaacagt 780

cattttagta tattaaatca cctaaagtat atgacttaat atatcaaaac aacaatttgt 840

ctgaggacat gtgaatatcc tagaagcaac tttttaaacc aaacaaatta ataagattaa 900

ctatataaat gcagcattta tatttgatgg tttctagtat caatgacatc caaaatacaa 960

acaagtgtt 969

<210>548

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>548

gtacactcct gtattagaag tagaaatcag cgag 34

<210>549

<211>37

<212>DNA

<213>Diabrotica virgifera

<400>549

gatgtcattg atactagaaa ccatcaaata taaatgc 37

<210>550

<211>754

<212>DNA

<213>Diabrotica virgifera

<400>550

gtacactcct gtattagaag tagaaatcag cgagtgttaa ttacttcgtt ctaaataatt 60

atttttgttg aagatttaat tcttactgat tctattgtct atctcaaata atatctgttt 120

aatgaagtat tatgaatagt tgtacttttt tagttaatat ttaataactt cactatccaa 180

tcaactatat aattcaagaa ccttaatgtg tccctaatat gtatgtccat gaatcaaatg 240

tgattcaagg atagaagcta ttctaaaatt aaaatagtat tggacataga ctcctgatta 300

tgttaacatt gggtcagtgg agttatcaat atcactaaga aatggcacat ttagaaaaaa 360

actaaaggta attagttttc aaatcgtaca attattgttc tcaaaacata agacagcatt 420

ctaatattct ttacctatta atactctatt ctgttttcaa ttgttttaaa tccataaata 480

aacagctgtc aaacaatcat aacattctac aaccaagaaa ccatgaaatg tccatgccac 540

attaatattg aaacaaattt tttggtttat gttacaacaa acagtcattt tagtatatta 600

aatcacctaa agtatatgac ttaatatatc aaaacaacaa tttgtctgag gacatgtgaa 660

tatcctagaa gcaacttttt aaaccaaaca aattaataag attaactata taaatgcagc 720

atttatattt gatggtttct agtatcatga catc 754

<210>551

<211>926

<212>DNA

<213>Diabrofica virgifera

<400>551

gtaagatata agagaataac acaatttgat aatatatttc ttcggttacc attcgtactt 60

aaatatacac tcgttctaat attatttttc acctaacctt tactaacttc tatatctaga 120

tccttatccg tcaaatacgg caacttaacc ttttcgtgga gggaaataac cgtagtcgct 180

aacacttttc ggtgcccggt agatggttaa atacaaagag gactcgttta ggtaccgtta 240

attatcgtaa ataccagtaa atgattcatt taggattaaa attacttgct aaagtaagct 300

gagtaagttt tatcggtgat aatattacag ccttctagtt tgtcaacgat acgccatcta 360

ggttttcaaa gatatattcc aggacccggt ataaaaccgt gggagaaata atacccacct 420

tgatgtattc ctcgatttac ggtgttttgt ctgcatctat ccagacttca tccaaatacc 480

atcactttca tgtatgtact tgataaggct cgaatgcctg gagtcaaatc ttgcttaatc 540

cagggcctta caaattgacc aaatttccaa gacttttcgg ttctacaaat ttaatctaca 600

caattaagtg tatggataca ttgtattctg cgtaatattt ctttattaca gttcttattg 660

cataaaggtg gttagtaata tttaacgcgt ttcaaaaacc cgcctgttcg acagtgaaga 720

gtgatcaatt caaatagcac agacctataa gtgatgtatt ggcaaccgta aactatattt 780

gttataatca aatcaataat tttataagct aaatcagggt agttctcgga agttggctgt 840

ataccattta gttaaaaaac tttcaacatc aaagtaaaaa aacttctgtt gtaatttgtg 900

aagtattaaa ttaaaaatcc aaacgg 926

<210>552

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>552

cttcggttac cattcgtact taaatataca ctcg 34

<210>553

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>553

ggtatacagc caacttccga gaactacc 28

<210>554

<211>804

<212>DNA

<213>Diabrotica virgifera

<400>554

cttcggttac cattcgtact taaatataca ctgttctaat attatttttc acctaacctt 60

tactaacttc tatatctaga tccttatctg tcaaatatgg caacttaacc ttttcgtgga 120

gggaaataac cgtagtcgct aacacttttg gtgcccggta gatggttaaa tacaaagagg 180

acttgtttag gtaccgttaa ttatcgtaaa taccagtaaa tgattcattt aggattaaaa 240

ttacttgcta aagtaagctg agtaagtttt atcggtgata atattacagc cttctagttt 300

gtcaacgata cgccatctag gttttcaaag atatattcca ggacccggta taaaaccgtg 360

ggagaaataa tacccacctt gatgtattcc tcgatttacg gtgttttgtc tgcatctatc 420

cagacttcat ccaaatacca tcactttcat gtatgtactt gataaggctc gaatgcctgg 480

agtcaaatct tgcttaatcc agggccttac aaattgacca aatttccaag acttttcggt 540

tctacaaatt taatctacac aattaagtgt atggatacat tgtattctgc gtaatatttc 600

tttattacag ttcttattgc ataaaggtgg ttagtaatat ttaacgcgtt tcaaaaaccc 660

gcctgttcga cagtgaagag tgatcaattc aaatagcaca gacctataag tgaagtattg 720

gcaaccgtaa actatatttg ttataatcaa atcaataatt ttataagcta aattagggta 780

gttctcggaa gttggctgta tacc 804

<210>555

<211>904

<212>DNA

<213>Diabrotica virgifera

<400>555

gcaaaagtta aacttttgaa ctattttgat ctatcgtcaa tgtttgcaga aaatatttga 60

ttaaatacaa ttgaaaaaac aatggaacat catagaaact tgatgaagac ttacaagaac 120

aacaagtact cggtcaaata aacattttgg aaagtttacc tttttaaaat agttggactt 180

tttttctgaa ctcctgccag gctctcgata atcttagaat aatgggttgt tttatgtata 240

cttgtttatt ggctgaggta tttaatatct agattaagat tccaatcaaa gtttttggcc 300

taggtgaact gtagttttat agaaaaatct caaacgcatt tagttgcgtt tataaatgtc 360

tactacctaa ttgtatagaa cgcacaggtt atattttaca gatttctcga ctttttgact 420

tctatccgtg atgatttgac gtgagaggcg tccaaagtct cttttgaaaa tttgtaaatg 480

tgttaaggtg ctttgagttc gaaagttagc tgtcttttcg aagcattaat ttgtcaaaaa 540

catagttata gtataaacca ataagcgctt acctcaggaa tatttgtctt gtagaggtaa 600

aatgaagagt tatattttta ttgttcgtca gagataaaag attttgtttt gtgagaatat 660

cgctaaatat tatatttttg atacattaat aaatatacag attgggcaag ctagtttttg 720

aataacatat tgacatttat ttatcggatt cactgtgttc agaagagcct ttggaaattt 780

agattagggt gcaagtatat taaagatgat aagagattga tacatatcat actatacata 840

tcatattaac tatatcgatg ttattctata ttataaaatt aatatttaat aaatgtctac 900

cagc 904

<210>556

<211>36

<212>DNA

<213>Diabrotica virgifera

<400>556

catagaaact tgatgaagac ttacaagaac aacaag 36

<210>557

<211>36

<212>DNA

<213>Diabrotica virgifera

<400>557

tctcttatca tctttaatat acttgcaccc taatct 36

<210>558

<211>725

<212>DNA

<213>Diabrotica virgifera

<400>558

catagaaact tgatgaagac ttacaagaac aacaagtgct ggtcaaataa acattttgga 60

aagtttacct ttttaaaata gttggacttt ttttctgaac tcctgccagg ctctcgataa 120

tcttagaata atgggttgtt ttatgtatac ttgtttattg gctgaggtat ttaatatcta 180

gattaagatt ccaatcaaag tttttggcct aggtgaactg tagttttata gaaaaatctc 240

aaacgcattt agttgcgttt ataaatgtct actacctaat tgtatagaac gcacaggtta 300

tattttacag atttctcgac tttttgactt ctatctgtga tgatttgacg tgagaggcgt 360

ccaaagtctc ttttgaaaat ttgtaaatgt gttaaggtgc tttgagttcg aaagttagct 420

gtcttttcga agcattaatt tgtcaaaaac atagttatag tataaaccaa taagcgctta 480

cctcaggaat atttgtcttg tagaggtaaa atgaagagtt atatttttat tgttcgtcag 540

agataaaaga ttttgttttg tgagaatatc gctaaatatt atatttttga tacattaata 600

aatatacaga ttgggcaagc tagtttttga ataacatatt gacatttatt tatcggattc 660

actgtgttca gaagagcctt tggaaattta gattagggtg caagtatatt aaagatgata 720

agaga 725

<210>559

<211>874

<212>DNA

<213>Diabrotica virgifera

<400>559

taataaataa atctacttta aaatgtacat ataatatatt atatctgaat tgtcaatatg 60

aatgagtgag ataaaattaa attattagag aaaaattttt taccaagcat caaaaacaaa 120

atttgattaa attattaaag ttttgtattt tgataatgat ttccaagttg gaaattgaaa 180

cgtcaaataa atttaattta aaacttaaac tgtggcttga tcccctgtca caagaaaaaa 240

aaacacacca cgtctacgtc tatgcagata aggacacctg ttaacgagta aaatatttct 300

tggattctca ccctgccttg gacgtaaata tatagaccag cgaagtattg ataaggttac 360

caatgattca cagtgttcaa ggaatttgtt caacttagcc gcccgtaaat atgacccata 420

gaccggaggt atattgttac cgaggcaaat tttatgaaat catcttcctt tttcccttat 480

gcacattggc aaggcatacc caataaaaat gaccccggta taaatttagt tttaaaattt 540

aacattgttg ctgttctcag ttcaatttgt agatgagtcg ccttcaatta aaaatggttg 600

gagagagaac aaatgattga gtaaattagt aaaaaaacat taattagtag tcatcttaat 660

tataaatcaa attaataata aaaatatccc aactaaaata ttttactaaa cgtcataccg 720

ttggtcatac taaacgtcat acatatttat acctaattat gacagaagtc aacaagcact 780

taagataacg tctaaatgtg agaaactact atgtaatgta aatgtatagg tgtctaacga 840

caattttcca cctctacaag tttgatctct tttt 874

<210>560

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>560

tgtggcttga tcccctgtca caag 24

<210>561

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>561

gagatcaaac ttgtagaggt ggaaaattgt cg 32

<210>562

<211>658

<212>DNA

<213>Diabrotica virgifera

<400>562

tgtggcttga tcccctgtca caagaaaaaa aaacacacca cgtctacgtc tatgcagata 60

aggacacctg ttaacgagta aaatatttct tggattctca ccctgccttg gacgtaaata 120

tatagaccag cgaagtattg ataaggttac caatgattca cagtgttcaa ggaatttgtt 180

caacttagcc gcccgtaaat atgacccata gaccggaggt atattgttac cgaggcaaat 240

tttatgaaat catcttcctc tttcccttat gcacattggc aaggcatacc caataaaaat 300

gaccccggta taaatttagt tttaaaattt aacattgttg ctgttctcag ttcaatttgt 360

agatgagtcg ccttcattaa aaatggttgg agagagaaca aatgattgag taaattagta 420

aaaaaacatt aattagtagt catcttaatt ataaatcaaa ctaataataa aaatatccca 480

actaaaatat tttactaaac gtcataccgt tggtcatact aaacgtcata catatttata 540

cctaattatg acagaagtca acaagcactt aagataacgt ctaaatgtga gaaactacta 600

tgtaatgtaa atttataggt gtctaacgac aattttccac ctctacaagt ttgatctc 658

<210>563

<211>849

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(765)

＜223〉n=a, c, g or t/u

<400>563

cttaaaatgt ataaatttac ctgattataa aacctttttt tatttcttaa caaataaaca 60

ttcagtggac caaaggatac atatcgatac ctcagagcaa cagtgagata agtggaaaat 120

ttcagttttt ttaagtctgc ttatttacat agaatgtgtt gccagtgtgg agtgcagtgc 180

attctatgta aatggcgtac ttaaacaagg ttgaggtttt ggacttctca ctgttgcttt 240

gatgtatcga tataagctac atagtagata catagaggaa atcttgtgct ttattctttt 300

ttagaatttc aatggttaat atattagata ttaaaataat gacccaaaaa atctatatag 360

tgctatctaa atattgctgc tgcaagaatt tttttattag ttctttaacc cagttttatg 420

accttcatgt acatatatcc aaatctatat tgccagggta ttggaaataa taccctggat 480

ttttagcctg aagtatatta ccaatgatgt acacaaacta ctagtctaaa gaaacaccga 540

ctattgaaga atattaagaa gctacaggtt acttttcatt tttccaaaat atcatttgta 600

tttttcagtt ctattctgtc acaactaaat tttattccac tttaccacga gagtaatctg 660

ttttttcgta actaaaagag gatcgatggg tagccaagaa gttgagaaac agtaagattt 720

caaatattgc aaaacgttgc aaatattttt aagaatgtat tttanaatgt taaatggata 780

catgaactaa tcagaaagca gtcttcaaaa aagaaaaagc agtgttaaga cacagtattc 840

gaaaacatc 849

<210>564

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>564

cattcagtgg accaaaggat acatatcg 28

<210>565

<211>35

<212>DNA

<213>Diabrotica virgifera

<400>565

gactgctttc tgattagttc atgtatccat ttaac 35

<210>566

<211>655

<212>DNA

<213>Diabrotica virgifera

<400>566

cattcagtgg accaaggata catatcgata cattctatgt aaatagcgta cttaaacaaa 60

attgaaattt tggacttctc actgttgctt tgatgtatcg atataagcta catagtagat 120

acatagagga aatcttgtgc tttattcttt tttagaattt caatggttaa tatattagat 180

attaaaataa tgacccaaaa aatctatata gtgctatcta aatattgctg ctgcaagaat 240

ttttttatta gttctttaac ccagttttat gaccttcatg tacatatatc ccaaatctat 300

attgccaggg tattggaaat aataccctgg atttttagca tgaagtatat taccaatgat 360

gtacacaaac tactagtcta aagaaacacc gactattgaa gaatattaag aagctacagg 420

ttacttttca tttttccaaa atatcatttg tatttttcag ttctattctg tcacaactaa 480

attttattcc actttaccac gagagtaatc tgttttttcg taactaaaag aggatcgatg 540

ggtagccaag aagttaagaa acagtaagat ttcaaatatt gcaaaacgct gcaaatattt 600

ttaagaatgt attttaaaat gttaaatgga tacatgaact aatcagaaag cagtc 655

<210>567

<211>848

<212>DNA

<213>Diabrotica virgifera

<400>567

cggacgcgtg ggtaatgatc ttaacgttta tagtactata tagagaattg aataaccttt 60

caaatgagcc ggcacacgac atccgttctc atttataaag aaaaaatcga ttacgtcatc 120

acgcccagat ggatcgccgt caccggtgcg atatatgtgt caagggtcat aattggaaag 180

tcgaactttt gtattttaca ttttttttct agttctgtaa ataaggcagt ttgcaggggg 240

gtcaaaatat ggtgaatagc cctgtacatt cattggggcc gaccgatcgg ctctgtcccg 300

tcatgcagct gaccgactgt aataactttt atttatattc aatttggaat gtgtgtactg 360

attttcaacc ttagtaaatg gatttaatta ccttcgtagg aaagcaactt tgataccctc 420

ttagtaacca ctggttctac gtttcgtttg accgaccgca gtatgtttct ctacctacac 480

aatcacagtg atcaattatg aaaagtttag cttttgtgaa ttcacagatt ttttagcgct 540

ttatattgga aaattaacat ttttcaatat aaaccgtcca ctatattaat agatacaaaa 600

ttaggagctg tagcagaaaa gtatctcaac tgactcaatt tattttatta ataagtcctg 660

gtatttatta cgtttgaaaa cggacatttt ggatgctttc tatcttcgat ctttgtaaag 720

aaaccgtgga ataaagtaaa acaatgttaa atattaccta ggtattttaa tgcataaaat 780

tatatactca atgcttgaag gataatataa agaagcattt gttttttttt taatattttt 840

tctcttcg 848

<210>568

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>568

cgattacgtc atcacgccca gatg 24

<210>569

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>569

ctttacaaag atcgaagata gaaagcatcc a 31

<210>570

<211>610

<212>DNA

<213>Diabrotica virgifera

<400>570

cgattacgtc atcacgccca gatggatgcc gtcaccagta cgatatatat gtcagggatc 60

ataattggaa aatcgaacct ttgtattttg catttttttt ctgattctgt aaataaagcg 120

gtttacaagg gggtcaaaat atggtgaatg gccctgtaca ttcattgggg ccgaccgatc 180

ggctctgtcc cgtcatacag ctgaccgact gtaataactt ttatttatat tcgatttaga 240

atgtgtgtac tgattttcaa ccttagtaaa tggatttaat taccttcgta ggaaagcaac 300

tttgttaccc tcttagtaac cactgttcta cgtttcgttt gaccgaccgc agtatgtttc 360

tctacctgca cagtcacagt aatcaattgt gaaaagttta gcttttgtaa attcacagat 420

tttttagcgc tttatattgg aaaattaaca tttttcaata taaaccgtcc actatattaa 480

tagatacaaa attaggagct gtagcagaaa agtatctcaa ctgactcaat ttattttatt 540

aataagtcct ggtatttatt acgtttgaaa acggacattt tggatgcttt ctatcttcga 600

tcttgtaaag 610

<210>571

<211>828

<212>DNA

<213>Diabrotica virgifera

<400>571

gacaataatc gtgtagatgg cgctaagatt ataatagatt atttataatt agatattacg 60

gaacattaaa aaaaacttaa attcagtatt taaaacgtaa gtatatttaa ggtaaaaata 120

tataccacag ctttgaccaa ctaatattgt ttataattaa tgtttttaat tttaatttta 180

aattaatcac tttgacattt atgtcaaatt tccgataaac gtttacaaac ttgtcactac 240

tgcgttcgcg aatttgtaaa tatcccctct acgtacgagc tcacagcgta tacgctgtgt 300

gcttcgctgg tgtcgttcct agcggactac tattcaactt tcaccggtaa tttttaaatt 360

tattatttaa ttgttatcgc ttaatattta caacgcaaaa aagtaattaa attgtaatgg 420

attttttaag attttgctaa tcattttgac gttctattga caaaatatga atttcttact 480

tcggatactt tcacaattat cgtgtagatg gcgctaagat taatggatta atagattaat 540

agaacattaa aaaaacttaa attcagtatt taaaacgtaa gcatatttaa ggtaaaaata 600

tataccacag ctttgactaa ctaatatttt ttataattaa tgtttttaat tttaatttta 660

aattaatcac tttgacattt atgtcaaatt tccggtaaag gtttacagac ttgtcactaa 720

tggcgttcgc gaatttgtaa atatcccctc tacgtacgag ctcacagcgt ataactgaac 780

gacaacgaac gaacgaacac gaacactgaa cacagattca cagatagc 828

<210>572

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>572

cgtttacaaa cttgtcacta ctgcgttcg 29

<210>573

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>573

gctatctg tgaatctgtgtt cagtgttcg 29

<210>574

<211>609

<212>DNA

<213>Diabrotica virgifera

<400>574

cgtttacaaa cttgtcacta ctgcgttcgc gaatttgtaa atatcccctc tacgtacgag 60

ctcacagcgt atacgctgtg tgcttcgctg gtgtcgttcc tagcggacta ctattcaact 120

ttcaccggta atttttaaat ttattattta attgttatcg cttaatattt acaacgcaaa 180

aaagtaatta aattgtaatg gattttttaa gattttgcta atcattttga cgtcctattg 240

acaaaatatg aatttcttac ttcggatact ttcacaatta tcgtgtagat ggcgctaaga 300

ttaatggatt aatagattaa tagaacatta aaaaaactta aattcagtat ttaaaacgta 360

agcatattta aggtaaaaat atataccaca gctttgacta actaatattt tttataatta 420

atgtttttaa ttttaatttt aaattaatca ctttgacatt tatgtcaaat ttccggtaaa 480

ggtttacaga cttgtcacta atggcgttcg cgaatttgta aatatcccct ctacgtacga 540

gctcacagcg tataactgaa cgacaacgaa cgaacgaaca cgaacactga acacagattc 600

acagatagc 609

<210>575

<211>824

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(785)

＜223〉n=a, c, g or t/u

<400>575

gcgatgcgat accgacaaag ctccctttga ctcggacgcg ccagaccgac agagaacgga 60

agtagagcga agcgaggcaa agttgcacaa ggtgtccgtc tacactctcg tacttgttga 120

acctcgatcg acttcggcga gagtgtggag ctcacataac attttttcat agagagtaat 180

aatgtaacag tttgtagttt ctaccagttg tgatgtaaca ggcgtaacgc tatgttattt 240

cacaatatgg agaagatcta ctacttcttc atttgtgacg agatctacca gcgaaatttc 300

aaaaattgtc ctcgagatgg acatttcaaa cttttttagg ttctatgatc ttaatttaac 360

ttccatcaac aataagattt actgtttttg attaaatagt taatctagca aagatagacc 420

agcaggtact tccttaaatc tagtaatggt cagcgtttac atagaccaat aatagtttat 480

taagcttttt tcggtcaact ttttaagtct tgtttttaaa ggaatgtttg tataacattt 540

taaacctttg taaaacatgt accattaatg ttgcccttct actcctttat aaaaataatt 600

gttaaaataa cttataaaat taatcttgtt taatgactcg aaatataaat tttaattttc 660

gtataaggtt cagatttaca gtattagtta tattataatt tacagaaatt tttcaatttc 720

ttttctaata actacacata cagcaaaaag tatgcctggt ggtaacgttc attaaattaa 780

gaagnttgtc gttgcttgaa acttgtgaaa attttgatag tttt 824

<210>576

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>576

cgatgcgata ccgacaaagc tcc 23

<210>577

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>577

cacaagtttc aagcaacgac aatcttc 27

<210>578

<211>804

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(27)..(165)

＜223〉n=a, c, g or t/u

<400>578

cgatgcgata ccgacaaagc tcccttngac tcggacgcgc cagaccgaca gagaacggaa 60

gtagagcgaa gcgaggcaaa gttgcacaag gtgtccgtct acactctcgt acttgttgaa 120

cctcgatcga cttcggcgag agtgtggagc tcacataaca ttttntcata gagagtaata 180

atgtaacagt ttgtagtttc taccagttgt gatgtaacag gcgtaacgct atgttatttc 240

acaatatgga gaagatctac tacttcttca tttgtgacga gatctaccag cgaaatttca 300

aaaattgtcc tcgagatgga catttcaaac ttttttaggt tctatgatct taatttaact 360

tccatcaaca ataagattta ctgtttttga ttaaatagtt aatctagcaa agatagacca 420

gcaggtactt ccttaaatct agtaatggtc agcgtttaca tagaccaata atagtttatt 480

aagctttttt cggtcaactt tttaagtctt gtttttaaag gaatgtttgt ataacatttt 540

aaacctttgt aaaacatgta ccattaatgt tgcccttcta ctcctttata aaaataattg 600

ttaaaataac ttataaaatt aatcttgttt aatgactcga aatataaatt ttaattttcg 660

tataaggttc agatttacag tattagttat attataattt acagaaattt ttcaatttct 720

tttctaataa ctacacatac agcaaaaagt atgcctgtgg taacgttcat aaattaagaa 780

gattgtcgtt gcttgaaact tgtg 804

<210>579

<211>812

<212>DNA

<213>Diabrotica virgifera

<400>579

cggagacgag tcatttgcgg acatgtgttt gtcgagcaaa ccggcattat tttttcatgt 60

agaattgccc tttaaagttt ttcatactta tttactaaca ccctgtatgt gtcgacaaca 120

catgtacaag tatgcatgca caatgcgcca ttttaaactt gaacatcagt agttgaagca 180

tccagtatcg tatgtctaca cgaatggtca aaactcaact gtattaaact ttgtacaagt 240

ctttcacaca cacactctca attacacttg cgtaaaaaat taacaagtaa aaattacaaa 300

atgttcacaa gtctcttgcc acttgtacag ttaacgtact tgtgctagca ttacccaaca 360

cccgttccag tatacttgtg caataaactt gtgcatggga acacgttggg aagaaaaatg 420

tgtaaggtta ggtcgaacac gttgaccatt cgcaaaatta aattccaaca gagggcgctc 480

aaaatttcaa agatggacga caactctagg aaaacaattc attttgtcat ttgaattcac 540

agttctaaaa cgttacatta aaatcattta caggagtgtt ttgccaaagt aaccactaag 600

ttttgcaact aagacatctt ataagttaaa gacgactcaa aattatgttt aaactgtatg 660

cattcattaa aatttgatgc taactactga tttgttttta cctttttttc ataaaaaaat 720

ctggcccccg ataatcacac agcgttctaa aaattattaa tctatcttag gatttctaat 780

tttgttttta atttaattaa taggtgcact ac 812

<210>580

<211>24

<212>DNA

<213>Diabrotica virgifera

<400>580

ggagacgagt catttgcgga catg 24

<210>581

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>581

cttagtggtt actttggcaa aacactcctg 30

<210>582

<211>599

<212>DNA

<213>Diabrotica virgifera

<400>582

ggagacgagt catttgcgga catgtgtttg tcgagcaaac cggcattgtt ttttcatgta 60

gaattaccct ttaaagtttt tcatacttgt ttgctaacac cccgtatgtg tcgacaacac 120

atgtacaagt atgcatgcac aatgcgccat tttaaacttg aacatcagta gttgaagcat 180

ccagtatcgt atgtctacac gaatggtcaa aactcaactg tattaaactt tgtacaagtc 240

tttcacacac acactctcaa ttacacttgc gtaaaaaatt aacaagtaaa aattacaaaa 300

tgttcacaag tctcttgcca cttgtacagt taacgtactt gtgctagcat tacccaacac 360

ccgttccagt atacttgtgc aataaacttg tgcatgggaa cacgttggga agaaaaatgt 420

gtaaggttag gtcgaacacg ttgaccattc gcaaaattaa attccaacag agggcgctca 480

aaatttcaaa gatggacgac aactctagga aaacaattca ttttgtcatt tgaattcaca 540

gttctaaaac gttacattaa aatcatttac aggagtgttt tgccaaagta accactaag 599

<210>583

<211>802

<212>DNA

<213>Diabrotica virgifera

<400>583

cgtggcgttt ttctatttac agtatgtgaa tgtgtcaaag taactgtgtt tttcaaaaat 60

taacaaaaga aattcaaaat gtacaagtgg acaaaaaata ttaaacaaat gtatatagtg 120

tatctatttc taatcgaatc aacaaaatat gatcttactg aacagaatat gatgcaagct 180

aatattaaat gcaactcgta tgttttatta tatcctaaac caaagactta ttcatcttga 240

aggtgactgt gtgccgtcat ctcttttatt gagacaatta ggacgttttt cgttctcagc 300

ggcttctcgg aaatccctgg cttacagaaa cggatggtga ctatagtcac ggaactgtca 360

aaattaattt tgtatcttat atgaagcctc aaacgttttg gaaatatttc ttctctttat 420

caggaactgg aaagaagtat atgccctcag tttgttatct ttctctagat gatgggagga 480

aaaattttta aaatacctgt ttgtcagagt tttattacta ttaagaagaa ttcttcatct 540

taaaggtaac cgggtgtcgt catcactttt attgacacaa ttaggacgtt ttttgatctc 600

aatggctttt ggtgtggaca atagttatag aactttcaaa accaattttg tgtcctgtct 660

agatcatcaa acggttttga gaagatttct tcccttcata tggagctgga aagaagtatt 720

tgacacctgt tagttatctt tctctagatg atggaaagaa aatctttttg aaagctatac 780

agaagtctta aaatacctgt tt 802

<210>584

<211>33

<212>DNA

<213>Diabrotica virgifera

<400>584

gtggcgtttt tctatttaca gtatgtgaat gtg 33

<210>585

<211>35

<212>DNA

<213>Diabrotica virgifera

<400>585

gataactaac aggtgtcaaa tacttctttc cagct 35

<210>586

<211>737

<212>DNA

<213>Diabrotica virgifera

<400>586

gtggcgtttt tctatttaca gtatgtgaat gtgtcaaagt aactgtgttt ttcaaaaatt 60

aacaaaagaa attcaaaatg tacaagtgga caaaaaatat taaacaaatg tatatagtgt 120

atctatttct aatcgaatca acaaaatatg atcttactga acagaatatg atgcaagcta 180

atattaaatg caactcgtat gttttattat atcctaaacc aaagacttat tcatcttgaa 240

ggtgactgtg tgccgtcatc tcttttattg agacaattag gacgtttttc gttctcagcg 300

gcttctcgga aatccctggc ttacagaaac ggatggtgac tatagtcacg gaactgtcaa 360

aattaatttt gtatcttata tgaagcctca aacgttttgg aaatatttct tctctttatc 420

aggaactgga aagaagtata tgccctcagt ttgttatctt tctctagatg atgggaggaa 480

aaatttttaa aatacctgtt tgtcagagtt ttattactat taagaagaat tcttcatctt 540

aaaggtaacc gggtgtcgtc atcactttta ttgacacaat taggacgttt tttgatctca 600

atggcttttg gtgtggacaa tagttataga actttcaaaa ccaattttgt gtcctgtcta 660

gatcatcaaa cggttttgag aagatttctt cccttcatat ggagctggaa agaagtattt 720

gacacctgtt agttatc 737

<210>587

<211>778

<212>DNA

<213>Diabrotica virgifera

<400>587

tcaaaaaatt catgaaagtc agatgacgat aaccaaggag ctgtcatgcg tctgcgcgaa 60

gaactaaaat gtttgacgcg ccgtcaccaa aatggaacat ggagaacaaa ggattttcgc 120

ggagtgaccc tactgattat ttagcattat actaatctca aatacattag gtttgttcta 180

gcatcagttt caagcggttt gaaatcatca gaagcatgcg catttgtttg atggcgttta 240

agcacacaaa ttactagttt gaaactacta gtccagcgtt ctaactttga tttggaaact 300

agctttaaac ggtcaaaagg gtttgcggat tttgttctga gtcaatttgt gacttgcgag 360

gtggaattat tgtttgaggt ttataaattt taaatgaaaa tgatatgttt tgactgtttg 420

ttacttacca ttatttacgt ttataacatt aattttctca caatggagca ctgaggaacg 480

tttgtttttc tatacttcca caaaattttg tggaagtatg gaaaaacaaa cgtttttcag 540

tgttttattg tgagataaaa tgaacttcca tcaagtaacg gtcgaatcca tcaattacat 600

tgattttcaa tatttaacgt aaatcttgaa aatttggata acacaagaaa aatatatttc 660

taatctaacc tcaaaattat aaataaaaac aaacatgaac cagtctcatt tgacgttagt 720

attttctccg aaaaaaaagc gttctagcaa aaaacagttt gctaccagtt tgtggatt 778

<210>588

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>588

catgaaagtc agatgacgat aaccaaggag 30

<210>589

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>589

ctaacgtcaa atgagactgg ttcatgtttg 30

<210>590

<211>709

<212>DNA

<213>Diabrotica virgifera

<400>590

catgaaagtc agatgacgat aaccaaggag ctgtcatgcg tctgcgcgaa gaactaaaat 60

gtttgacgcg ccgtcaccaa aatggaacat ggagaacaaa ggattttcgc ggagtgaccc 120

tactgattat ttagcattat actaatctca aatacattag gtttgttcta gcatcagttt 180

caagcggttt gaaatcatca gaagcatgcg catttgtttg atggcgttta agcacacaaa 240

ttactagttt gaaactacta gtccagcgtt ctaactttga tttggaaact agctttaaac 300

ggtcaaaagg gtttgcggat tttgttctga gtcaatttgt gacttgcgag gtggaattat 360

tgtttgaggt ttataaattt taaatgaaaa tgatatgttt tgactgtttg ttacttacca 420

ttatttacgt ttataacatt aattttctca caataaagcg ctgaagggcg tttgtttttc 480

tatacttcca caaaattttg tggaagtatg gaaaagcaag cgtttttcag tgttttattg 540

tgagataaaa tgaacttcca tcaagtaacg gtcgaatcca tcaattacat tgattttcaa 600

tatttaacgt aaatcttgaa aatttggata acacaagaaa aatatatttc taatctaacc 660

tcaaaattat aaataaaaac aaacatgaac cagtctcatt tgacgttag 709

<210>591

<211>765

<212>DNA

<213>Diabrotica virgifera

<400>591

gacaatttgg aatttaattc tttggaatcg ttgtcagtgg actttcataa ttttttacat 60

aatttaattt tacggtagaa taattttttc aacgttactg tttagtgctg gcctttaaac 120

gaatgtgatt ctagatgata gttgttaaat gatcgtcaag tcgaatagtg gtgatctctg 180

agagtctcct gaagtagtaa ggcagataaa tgaatagctg tgagtttgtt gaaataagtg 240

tcctgacgga ggctcatcac gtaaagcatt gtaagttata ttgttctact tatattccaa 300

gagtcaccgt tgcatgccgg aacgagaaat agattcagtt tattgagagg agaaaaggct 360

agcatggttt tttgaacgat acgtgcatct gaagggggtc attgatgaca ataggcttgc 420

aatagtttgt tgcgagattg ctgactacat agggggctgc taagagtaaa ttgtaggcga 480

ccagagacaa gaatttggac aactcatcat ccgagagaca gttttatttt ttttgtagaa 540

ttattcataa cgcaaatttc aataagtact ttagatagtg gtaggagtat ttcaataatc 600

agaataggag atattatttt tagaggatat tttgagggtg aatttatttc aatagatgtt 660

tttgtaccat atatgtgttt tattccgtta gtctttgacc ttatttatca tatgtaaagc 720

aaaggagata ttgaagcacg agaatataaa accctgagat tagag 765

<210>592

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>592

ctttggaatc gttgtcagtg gactttc 27

<210>593

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>593

ctcgtgcttc aatatctcct ttgctttac 29

<210>594

<211>726

<212>DNA

<213>Diabrotica virgifera

<400>594

ctttggaatc gttgtcagtg gactttcata attttttaca taatttaatt ttacggtaga 60

ataatttttt caacgttact gtttagtgct ggcctttaaa cgaatgtgat tctagacgat 120

agttgttaaa tgatcgtcaa gtcgaatagt ggtggtctct gagagtctcc tgaagtagta 180

aggcagataa atgaatagct gtgagtttgt tgaaataagt ttcctgacgg aggctcatca 240

cgtaaagcat tgtaagttat attgttctac ttatattcca agagtcaccg ttgcatgccg 300

gaacgagaaa tagattcagt ttattgagag gagaaaaggc tagcattgtt ttttgaaaga 360

tacgtgcatc tgtagggggt cattgatgac aataggcttg caatagtttg ttgcgagatt 420

gctgactaca tagggggctg ctaagagtaa attgtaggcg accagagaca agaatttgga 480

caactcatca tccgagagac agttttattt ttttttttgt agaattattc ataacgcaaa 540

tttcaataag tactttagat agtggtagga gtatttcaat aatcagaata ggagatatta 600

tttttagagg atattttgag ggtgaattta tttcaataga tgtttttgta ccatatatgt 660

gttttattcc gttagtcttt gaccttattt atcatatgta aagcaaagga gatattgaag 720

cacgag 726

<210>595

<211>735

<212>DNA

<213>Diabrotica virgifera

<400>595

ttattttagc acacaaacaa aatgctcggt gttagtatat tatagcatca atgtttcgaa 60

ttttgcacga tccctcttca ggtgattcga aaaacatgaa tgcgtttaaa aataattcga 120

tcgaaatttt gcgcctacgc tctcaaaaaa ggttaaagta ttatacattt ttgtaatcag 180

tatttcaatt gcttttaaat cagctgtcat atgatgtact tttccatttt aaaaaaatta 240

aagttgtgcc tgtcacctga agagggatcg catgaagttc gaaacattga tgttataata 300

cactttgatc attttaaaaa tcgacctgtc cgagcgtttt gcttatgtgc taaaaataaa 360

taagttaata gggggggggg gtaacaatca ttccagcgca ctgtatggta actacgaggc 420

aagtagaagc tgtacctgga atcaacataa ttagcgttta agccattttg aagaacaaat 480

tccattcata taaaatgtac acctagtaca gaaacttaat gagaatgatt atgaccgacg 540

tttacagttt tgtgaaataa ttagtgagcg agcagtgagt gatccacagt tgcgctttaa 600

tattttttct gcggtgaata cactgttctc ataaaaaatg gaactagtgc aattactgga 660

gatggatatg agctattacc tccgatttcg ttgaacgaaa aatcgtcaca attgccgtta 720

ttggtctgat aaaac 735

<210>596

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>596

ctcggtgtta gtatattata gcatcaatgt ttcg 34

<210>597

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>597

gaaatcggag gtaatagctc atatccatct c 31

<210>598

<211>662

<212>DNA

<213>Diabrotica virgifera

<400>598

ctcggtgtta gtatattata gcatcaatgt ttcgaacccc gcacgatccc tcttcaggtg 60

actcgaaaaa catgaatgcg tttaaaaata attcgatcga aattttgcgc ctacgctctc 120

aaaaaggtta aagtattata catttttgta atcagtattt caattgcttt taaatcagct 180

gtcatatgat gtacttttcc attttaaaaa aattaaagtt gtgcctgtca cctgaagagg 240

gatcgcatga agtccgaaac actgatgcta caatacactt tgatcacttt aaaaatcgac 300

ctgtccgagc gttttgctta tgtgctaaaa ataaataagc caataggggg ggggtaacaa 360

ttattccagc gcactgtatg gtaactacga ggcaagtaga agctgtacct ggaatcaaca 420

taattagcgc ttaagccatt ttgaagaaca aattccattc atataaaatg tacacctagt 480

acagaaactt aatgagaatg attatgaccg acgtttacag ttttgtgaaa taattagtga 540

gcgagcagtg agtgatccac agttgcgctt taatattttt tctgcggtga atacactgtt 600

ctcataaaaa atggaactag tgcaattact ggagatggat atgagctatt acctccgatt 660

tc 662

<210>599

<211>711

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(525)..(705)

＜223〉n=a, c, g or t/u

<400>599

cacactatat ttatttgtga aatgcaagct aacgattgaa aacgatcggt catacacata 60

tattatgtgt cacataactt tggagaggta aaaaaatact cagaaaaact aaaaataata 120

aaaacaaaga acgtcttttc ctcttagttc ataaaggagc aaaattagga aagaaaaccc 180

tatcccacca acacctcttg taaaaatata aacggttaag acaagcagtg gatcttatgt 240

gtcaaattca cgtctattcc acgaattttc tgtttaagta aattgtagat ctttggttgt 300

atatatagtt acttttgttt catctaaaag ttatctacat taaccatata aaatgtttct 360

gaaatgttat tgaagaatta agatttcaag aagaatgtct ccaactaaaa tcgattttgg 420

taaaatcttg ttttgttaca agtgtacaaa tgtttgatgc aaaatatcta ttatgaggtt 480

gtactaaatt gtgagtatat ttaaaaataa aaaatactgt atctncttaa anaaccattc 540

aataaaaaaa taatttctta atttccccca aaaactttaa aattaaatta ataacttttt 600

cttatatcta ttatatctnc ttataatacc accacactca attttaaaaa tcttccaatc 660

attcttttat naatttttan tacaaatata aatttttttt ttacnaactt t 711

<210>600

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>600

gaaatgcaag ctaacgattg aaaacga 27

<210>601

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>601

cgattttagt tggagacatt cttcttgaaa tc 32

<210>602

<211>395

<212>DNA

<213>Diabrotica virgifera

<400>602

gaaatgcaag ctaacgattg aaaacgatcg gtcatacaca tatattatgt gtcacataac 60

tttggagagg tgttaggata ctcagaaaaa ctaaaaataa taaaaacaaa gaacgtcttt 120

tcctcttagt tcataaagga gcaaaattag gaaagaaaac cctatcccac caacacctct 180

tgtaaaaata taaacggtta agacaagcag tggatcttat gtgtcaaatt cacgtctatt 240

ccacgaattt tctgtttaag taaattgtag atctttggtt gtatatatag ttacttttgt 300

ttcatctaaa agttatctac attaaccata taaaatgttt ctgaaatgtt attgaagaat 360

taagatttca agaagaatgt ctccaactaa aatcg 395

<210>603

<211>699

<212>DNA

<213>Diabrotica virgifera

<400>603

ccgattttaa gaattttcgt ctataagtta caacatttta ccaaaatact gaaaaagaac 60

tgattagtct attgaaataa ctttaaaatg agttgaagta aaatagaatt ggagctttgt 120

ttatcaataa acatcaatga aaggtataca tttgcgatat gccccgtgtt ggcttaatcc 180

gttactgttt aaatttattt cttacgtttt aacctaacta cctgaggtat tagatttaat 240

ccatggtttc gaattcacct gtagaaggtt tcgagttggc agattcaggt agtagaaaag 300

ttacgaattg gccggtgttc tttgtatacc gtagtcccta gagataacgg actaccctgc 360

tttaatccca aagccgcgat agcgctataa aagggagact attattatta tcattttcta 420

tgttgcgttt cctttccgaa ccataaatac caaaaaaatt tgctactttt tgttcaatcg 480

taaatcaatc aatcagtcgt aaatcagaaa attaattaat gccggtacat ggacttcaag 540

gattaaagac aatcacaggt aagatatcag tcattgaaag atagtatagg agtatagcat 600

tgcattctgt ggatgaggca acatatttgg ggaaatataa gtttcttttt attcaattgc 660

gaacaattta agcaacattt tagtacaaac ttttttctt 699

<210>604

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>604

ggtatacatt tgcgatatgc cccgtg 26

<210>605

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>605

ccaaatatgt tgcctcatcc acagaatg 28

<210>606

<211>488

<212>DNA

<213>Diabrotica virgifera

<400>606

ggtatacatt tgcgatatgc cccgtgttgg cttaatccgt tactgtttaa atttatttct 60

tacgttttaa cctaactacc tgaggtatta gatttaatcc atggtttcga attcacctgt 120

agaaggtttc gagttggcag attcaggtag tagaaaagtt acgaattggc cggtgttctt 180

tgtataccgt agtccctaga gataacggac taccctgctt taatcccaaa gccgcgatag 240

cgctataaaa gggagactat tattattatc attttctatg ttgcgtttcc tttccgaacc 300

ataaatacca aaaaaatttg ctactttttg ttcaatcgta aatcaatcaa tcagtcgtaa 360

atcagaaaat taattaatgc cggtacatgg acttcaagga ttaaagacaa tcacaggtaa 420

gatatcagtc attgaaagat agtataggag tatagcattg cattctgtgg atgaggcaac 480

atatttgg 488

<210>607

<211>688

<212>DNA

<213>Diabrotica virgifera

<400>607

aaagttaaca acaataaaca gaataattta atttgaaccg atttttttag tgcaaacaag 60

taattggact tcgtaagtcc taggttttca cccaaagtaa tcgtcagtag aactgtaatt 120

cgatatttga actcttcgtg taactttgcg tcgatggata tacgatttta ctaattttga 180

gtcattttta ctaaactttg ggttatcatt gtcacagaaa tgacttcaaa accggaacta 240

aatattgaaa ctcaactttt caatacgctt cgattgatgt gttacatgta ctatttctgc 300

gactataatg gcacttctgg ttagaacttt ttaaccggaa atgatataaa aaccaaaact 360

ttatatttgt tctcatcttg ctaaggcacg tcaaatgata tatcgcttac actatttcgg 420

agactttaaa acagtaccta tgattgtaac tctaaaatcg gaagtcagat gtaaagtttc 480

tcatctttaa taccatcctt gggttttaag ctttcattcg acacatcatt tgtcattctg 540

tctgtattaa taacggagga gttttattcg cggacggact gacaaacagt catgaaacct 600

aaagtatata tttgttctcg actaatatat cacttgcact attccgatgc cgttaaaata 660

atacttctgg tcgtatttct aaaaccgc 688

<210>608

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>608

cgatatttga actcttcgtg taactttgcg tc 32

<210>609

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>609

ccagaagtat tattttaacg gcatcggaat ag 32

<210>610

<211>550

<212>DNA

<213>Diabrotica virgifera

<400>610

cgatatttga actcttcgtg taactttgcg tcgatggata tacgatttta ctaattttga 60

gtcattttta ctaaactttg ggttatcatt gtcacagaaa tgacttcaaa accggaacta 120

aatattgaaa ctcaactttt caatacgctt cgattgatgt gttacatgta ctatttctgc 180

gactatgatg gcacttctgg ttagaacttt ttaaccggaa atgatataaa aaccaaaact 240

ttatatttgt tctcatcttg ctaaggcacg tcaaatgata tatcgcttac actatttcgg 300

agactttaaa acagtaccta tgattgtaac tctaaaatcg gaagtcagat gtaaagtttc 360

tcatctttaa taccatcctt gggttttaag ctttcattcg acacatcatt tgtcattctg 420

tctgtattaa taacggagga gttttattcg cggacggact gacaaacagt catgaaaccg 480

aaagtatata tttgttctcg actaatatat cacttgcact attccgatgc cgttaaaata 540

atacttctgg 550

<210>611

<211>973

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(943)

＜223〉n=a, c, g or t/u

<400>611

gagcaataaa agaattcgca gtttgagagg aaagtttcaa caccccgtat cttggaaacg 60

aagcatttgc ggacatacgt ttatagagca aactgtcatt atgtaacacc ctgtataggc 120

ccacacaaaa aattattacc tccttcatga gacttttttt tattgagtta ttcatgtcgg 180

gttggacagc gtttctggtg tgaaaaattt tcgggagggg gacggttcgt aaatatgggg 240

gtttataaac tttggtgcat ctatggtggt tcctgtttat ctgaaccatc tcagatagtt 300

aaggggtggt taccccctac tataggggtt gatgaagtaa cactactcac agtatattca 360

tttatttata agttgaagta actacggtaa agagttggtg gctaaatatt tatccgctgt 420

gatgtttacc cgctgggatc tcaaataccc atgattaatc tttacgcaat gaatttagaa 480

cgtcgaatgt ggaatcccct gtttatgttt tggtatatat aaaaagtgaa agtgtttgta 540

ttataaatgc tgaatcaact tgattataaa taaagaataa tatttgctac tacagctgac 600

cttgcattat cgataactga tacacttgta tagtaatata ggtcaatcat gtttatgata 660

aacaacgcgt attcgtttac caaggacatt taaatattaa aagaattagt cttgccctta 720

ataagatggt tactaagaga tgctgtgaat cccaatacac atccgacaac tggagtaagt 780

atccttgaaa atttgtcgga aaatattacc agtgaattgt atttttatca aaatattctg 840

caaaaaaacc catcatttat gactctatag tggacgcaca tatccacgta tagtccccac 900

atatgagggg tgcatcataa agagtaataa attataccca ttngtaacat tgagttacca 960

agtgtcaaaa atc 973

<210>612

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>612

gcagtttgag aggaaagttt caacacc 27

<210>613

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>613

ggatatgtgc gtccactata gagtcataaa tg 32

<210>614

<211>867

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(771)

＜223〉n=a, c, g or t/u

<400>614

gcagtttgag aggaaagttt caacaccccg tatcttggaa acgaagcatt tgcggacata 60

cgtttataga gcaaactgtc attatttaac accctgtata ggcccacaca aaagattatt 120

acctccttca tgagactttt ttttttattg agttattcat gtcgagttgg acgtttctga 180

tatgaaaaat tttcaggagg gagacggttc gtaaatatgg gggtttataa actttggtgc 240

atctatggtg gttcctgttt atctgaacca tctcagatag ttaaggggtg gttaccccct 300

actatagggg ttgatgaagt aacactactc acagtatatt catttattta taagttgaag 360

taactacggt aaagagttgg tggctaaata tttatccgct gtgatgttta cccgctggga 420

tctcaaatac ccatgattaa tctttacgca atgaatttag aacgtcgaat gtggaatccc 480

ctgtttatgt tttggtatat ataaaaagtg aaagtgtttg tattataaat gctgaatcaa 540

cttgattata aataaagaat aatatttgct actacagctg accttgcatt atcgataact 600

gatacacttg tatagtaata taggtcaatc atgtttatga taaacaacgc gtattcgttt 660

accaaggaca tttaaatatt aaaagaatta gtcttgccct taataagatg gttactaaga 720

gatgctgtga atcccaatac acatccgaca actggagtaa gtatccttga naatttgtcg 780

gaaaatatta ccagtgaatt gtatttttat caaaatatac tgcaaaaaaa cccatcattt 840

atgactctat agtggacgca catatcc 867

<210>615

<211>967

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(784)..(895)

＜223〉n=a, c, g or t/u

<400>615

aatgtgatat ttaactagtg atttattttg tatttatcat atgttttata atttaacgaa 60

aacgtatctt tagactgttt gcaagagttg ttcatccact gcatggactt ttgggtaaaa 120

gacgtatagc aggttttaca aagcttcggt aattggacac atatttgaca ttaatcagtc 180

aactgtcata caacatactc atttttattt caaggaaata atcttagaat aagtattttt 240

tgcgtaaaca tgttatatga cgtataatag ataatatgat gtatattcca tagacatata 300

tattattatc atagactgag cctaccctcc gcgcttcctg gcgacaagat ctcttggaca 360

ggtttgcggt atctctttct agcacattgt atcgagaaac taacatgaaa ctgagtgggg 420

gtataggcac ggaaagggag gactactgtc gcagctttac tatgcataag agtgaaacag 480

cactgagcca caaaaaaaaa gatggtgtcg tcacttcgcg caacatgaca ctctatgata 540

atgtgtgtgt ctatggtata tcccagtgga catcctaagt cttgacgtca caaaattggg 600

agtagcttat atttggctcc aggcaattct gtttgtgatg ttaagcactc atagggaatt 660

tttaatttat tgtttatgtg gtttgtgtga caaaataaga cttttcattt gggtatttag 720

ttggagagac gtgccggtta agagattttt attcgttttt gcccaggtga gttaatttaa 780

ttanatgatt agaatgtaaa cagttttgag gttatgttta tttttcatca ctaaggaata 840

aaaaacacct gagcnaaaac gaataaaaag tctcctagtt gacacgtttc tttgncggaa 900

tacctaaatg gagtcttatt ttgccgcaca aagcacataa atgaaaaatt ccttatgacc 960

atttgac 967

<210>616

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>616

gcaagagttg ttcatccact gcatg 25

<210>617

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>617

gtgcggcaaa ataagactcc atttaggt 28

<210>618

<211>846

<212>DNA

<213>Diabrotica virgifera

<400>618

caagagttgt tcatccactg catggacttt tgggtaaaag acgtatagca ggttttacaa 60

agcttcggta attggacaca tatttgacat tgatcagtca actgttatac aacatactca 120

tttttatttc aaggaaataa tcttagaata agtatttttt gcgtaaacat gttatatgac 180

gtataataga taatatgatg tatattccat agacatgtat attattatca tagactgagc 240

ctaccctccg cgcttcctgg cgacaagatc tcttggacag gtttgcggta tctctttcta 300

gcacattgta tcgagaaact aacatgaaac tgagtggggg tataggcacg gaaagggagg 360

actactgtcg cagctttact atgcataaga gtgaaacagc actgagccac aaaaaaaaag 420

atggtgtcgt cacttcgcgc aacatgacac tctatgataa tgtatgtgtc tatggtatat 480

cccagtggac atcctgagtc ttgacgtcac aaagttggga gtagcttata tttggctcca 540

aacaattctg tttgtagtgt taaacactca tagggaattt ttaatttatt gtttatgtgg 600

tttgtgtgac aaggtaagac ttttcatttg ggtatttagt tggagagacg tgccagttgg 660

gagattttta ttcgtttttg cccaggtgag ttaatttaat taaatgatta gaatgtaaac 720

agttttgagg ttatgtttat tttccatcac taaggaataa aaaccacctg agcaggaacg 780

aataaaaaat ctcttagttg acacgtttct ttgacggaat acctaaatgg agtcttattt 840

tgccgc 846

<210>619

<211>967

<212>DNA

<213>Diabrotica virgifera

<400>619

acaattatta aaaataatag ttagtaataa aataatgaca aaaatttctt taggctattg 60

cagggtgggc tttaaacttt gagttggtca ctttatgact tttataataa taatttttaa 120

tcgagttatt aagccttgaa aatggccatt ttcgcgtttt tcaaatttga aattgcatgt 180

aactcggcaa cagtcaattt tatagaaaaa tcacaagata ccttttttgc tcagcttgat 240

ccagaaaatc taaaacaagt ttgtccgaag tgaaaaaatt gattttttga attcgtttaa 300

aaaaatgtgt agaacaactt tccgaccgcg gtactgcctg gcaccttgtg aatttgttat 360

aaggacctgt ttttgagtaa gtttgtgcaa aaaaattaat cggaataatt tacctaacgg 420

gacaagcata cagcgtggac tatttgcatt atgagccaaa cgtaaatggt ttgtaaaata 480

cactaaacac aaaaaaaatt agcagccatc gccaaaaaaa gttatacgta cctattaaaa 540

aacaaaaaaa atgaggttat aatatgtaca ctcgaccttc gggtccgtaa aaatggatgt 600

tctgtaaaag cctcagctgt gcgtgtgaat tttttgtaat ttataattta ataattacta 660

actgttctaa atacgaaatg agccacagtt tggctaaaaa atgatttttt ctacaaccgt 720

gttaaaaatg caatttttag cactccatac gtgcgttaaa aatgctactt taagacacta 780

gtgctttaaa atatttttaa ggcactgcag ttcgtattga ccgtatagac aattttgatg 840

taatgtcaaa aaaatataaa aatggaatgt cagtcacgtt caaagtaaaa gttttgtaga 900

tattgtcctg taatttacgt tgtaaacaaa tattgtatga gatgttaaag gtacattttt 960

aggcact 967

<210>620

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>620

gctcagcttg atccagaaaa tctaaaacaa g 31

<210>621

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>621

ctgtggctca tttcgtattt agaacagtta gt 32

<210>622

<211>458

<212>DNA

<213>Diabrotica virgifera

<400>622

gctcagcttg atccagaaaa tctaaaacaa gtttgtccga agtgaaaaaa ttgatttttt 60

gaattcgttt aagaaaatgg ttaaaacaac tttccgaccg cggtactgaa tggcaccttg 120

tgaatttgtt ataaggacct gtttttgagt aagtttgtgc aaaaaaatta atcggacaag 180

cggattaacg ggacaagcat acagcgtgga ctatttgcat tatgagccaa acgtagatgg 240

tttgtaaaat acactaaaca caaaaaaatt agcagccatc gccaaaaaag ttatacgtac 300

ctattaaaaa acaaaaaaaa atgaggttat aatatgtaca ctcgaccctc gggtccacaa 360

aaatagatgt tctgtaaaag cctcagctgt gcgtgtgaat tttttgtaat ttataattta 420

ataattacta actgttctaa atacgaaatg agccacag 458

<210>623

<211>1376

<212>DNA

<213>Diabrotica virgifera

<400>623

cggacgcgtg ggttcaagca actgttagcc ttgatcctgg ggataacagt catggcgttc 60

gtcaccaacg ctttcgagtg atggcgacca caaaacatta tcacaaatgt acttgaagca 120

atagtttatt tgaaataatc agaaatttcc ataattagtg gttagtatta ttagttgcgg 180

ttttattaat aagtgtcaac tgtcatttgt caaaatgtca actgtcattt gtcaaaatgt 240

catctgttat ttgtcaaaat gacagatgac aataactcaa ctgtcagttg tcaaaaagat 300

ggcgtaaaaa tatataaatt agttctgtag agtataaaag tcctttatgt aattcttact 360

ttcaaataaa ctcttgcact gacaaattaa atgtaatttt gacagtttct gaaaggtttg 420

actaaatgta aatgtcagaa ttatatatct gttgattttt atacaggcac atcaaacata 480

ttaacataca caattgaaga tcgttgtaac tgatatctgc agtcaacaat ttggaaattg 540

cttcacagct aactcaaatt agaagttatg tttcacaact gcataaaata attgttaaca 600

ggatcaatat tttattgttt atattagttt ttgtaaagat atgtttcact agtacttaaa 660

tatcgatcag agacacaaac tgtgttagaa aaggtccatg gaatgtatca atatgatttt 720

attttttata tcaccttcat ttgtgcattc cggtctcaat aattaatgta aaacgtcaaa 780

ttttcgtttc aggcgacaat tactgatact gcctacaaat agtggtataa ggcttttcat 840

cgattgtcat ttgtttcgag cttcttctgt catctagcag gcaagaccgc ctaaatgcgg 900

tataatttct agctatttca gctatgttta ggtatgcagc atgcaaacct gcctttggtc 960

gtatactact aagtgtactt gtaaatattt ggttaacgct aattgattgg ctctattaag 1020

tcaaaagata tcaagctttg aatttttcca ttgaaaataa aattggcctg ctagagggat 1080

aatctgtgta taatattaat atacacggat tataccacat attatgacag aagctcgaaa 1140

taaatgactg aatgaaaagc cctatggagc gagacttgtc agaacatgtt ttggtttcac 1200

ctaaccttac ataacctaca atttatctga atagctgtct attgagatat attttgtctg 1260

ccactgtctt ttttatgaat aacaattact gttttaccta aaaacaatta aaaaacatgt 1320

gataatgcgc gcgtacgcat ttaatatttt attaatatgc atacttcctc gtgccg 1376

<210>624

<211>26

<212>DNA

<213>Diabrotica virgifera

<400>624

ggataacagt catggcgttc gtcacc 26

<210>625

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>625

catgttctga caagtctcgc tccatagg 28

<210>626

<211>1148

<212>DNA

<213>Diabrotica virgifera

<400>626

ggataacagt catggcgttc gtcaccaacg ctttcgagtg atggcgacca caaaacatta 60

tcacaaatgt acttgaagca atagtttatt tgaaataatc agaaatttcc ataattagtg 120

gttagtatta ttagttgcgg ttttattaat aagtgtcaac tgtcatttgt caaaatgtca 180

actgtcattt gtcaaaatgt catctgttat ttgtcaaaat gacagatgac aataactcaa 240

ctgtcagttg tcaaaaagat ggcgtaaaga tatataaatt agttctgtag agtataaaag 300

tcctttatgt aattcttact ttcaaataaa ctcttgcact gacaaattaa atgtaatttt 360

gacagtttct gaaaggtttg actaaatgta aatgtcagaa ttatatatct gttgattttt 420

atacaggcac atcaaacata ttaacataca caattgaaga tcgttgtaac tgatatctgc 480

agtcaacaat ttggaaattg cttcacagct aactcaaatt agaagttatg tttcacaact 540

gcataaaata attgttaaca ggatcaatat tttattgttt atattagttt ttgtaaagat 600

atgtttcact agtacttaaa tatcgatcag agacacaaac tgtgttagaa aaggtccatg 660

gaatgtatca atatgatttt attttttata tcaccttcat ttgtgcattc cggtctcaat 720

aattaatgta aaacgtaaaa ttttcgtttc aggcgacaat tactgatact gcctacaaat 780

agtggtataa ggcttttcat cgattgtcat ttgtttcgag cttcttctgt catctagcag 840

gcaagaccgc ctaaatgcgg tataatttct agctatttca gctatgttta ggtatgcagc 900

atgcaaacct gcttttggtc gtatactact aagtgtactt gtaaatattt ggttaacgct 960

aattgattgg ctctattaag tcaaaagata tcaagctttg aatttttcca ttgaaaataa 1020

aattggcctg ctagagggat aatctgtgta taatattaat atacacggat tataccacat 1080

attatgacag aagctcgaaa taaatgactg aatgaaaagc cctatggagc gagacttgtc 1140

agaacatg 1148

<210>627

<211>1142

<212>DNA

<213>Diabrotica virgifera

<400>627

tttattgtat taatgtataa tgtgcagtat cattcagacc tagtatttaa agaatttatt 60

ttaagttttt attcatacac acattttttt atgattatac tagttaatgc tgttaagtca 120

tagataagat aggtagatgt taagtcatgt ttgttctcat tttttgtaac ttcggcttgt 180

aatatttgtg gaagttaaaa agtagattgt ttcattttct attagtacaa aaatgttgaa 240

caacaattca atattttaca aaagtcaagt ggttttaatg tatctttggt ttatttaaca 300

aaaaaattta attggataga aattgcatat attctgaaaa ccatgctata actgccagat 360

ttgtgaacaa acaatagcaa gttcctaaga agaacttccg tagcagctat acaaatttaa 420

acctctgttt aaaaaatctg aattaagagt atatgatata ggcacataaa agtgatgtcg 480

aaattaggag tacaaaatgt taatttttaa taattaattt ggcatatgag actaactgaa 540

ggcaaacaaa acttaataac atcataaaat cattgcaata aaaaactgaa aactttttgt 600

cttccatgtt tttatgtggc gcagaaggta caaaaacgta aatctgtaat ttaaaacaat 660

tactgttttt ttatacgtag ttctggcaaa gttttacatt tgcattttag aaaaatgtgt 720

ttcatgttgt gtagtaaagc caaatttagt taactgtaaa aataaattac aaatttattt 780

tggttgtgaa ttggcccgaa aatttttttt atatctacat ttattcagtt ttgccaaact 840

actaacagat acgtattttc gaatatcctt ttgattttaa caaagagcat tgattatttt 900

aagcttctag atgtgaaata aacggtcaac cacaaaaaaa aaaaaaaagg gcggccgccc 960

tttttttttt tttttaacat aatagtgaaa tctttatttt aatacatatt atagtgacac 1020

attataaagc caatacatta acggcttata acgcatttgt cgttactcga tccatctact 1080

tgtgacagtt ggaagctgac gaagatattc gcagcttgtc cttcaagtgg gtcaatatca 1140

tt 1142

<210>628

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>628

ccatgctata actgccagat ttgtgaac 28

<210>629

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>629

gtggttgacc gtttatttca catctagaag c 31

<210>630

<211>593

<212>DNA

<213>Diabrotica virgifera

<400>630

ccatgctata actgccagat ttgtgaacaa acaatagcaa gttcctaaga agaacttccg 60

tagcagctat acaaatttaa acctctgttt aaaaaatctg aattaagagt atatgatata 120

ggcacataaa agtgatgtcg aaattaggag tacaaaatgt taatttttaa taattaattt 180

ggcatatgag actaactgaa ggcaaacaaa acttaataac atcataaaat cattgcaata 240

aaaaactgaa aactttttgt cttccatgtt tttatgtggc gcagaaggta caaaaacgta 300

aatctgtaat ttaaaacaat tactgttttt ttatacgtag ttctggcaaa gttttacatt 360

tgcattttag aaaaatgtgt ttcatgttgt gtagtaaagc caaatttagt taactgtaaa 420

aataaattac aaatttattt tggttgtgaa ttggcccgaa aatttttttt atatctacat 480

ttattcagtt ttgccaaact actaacagat acgtattttc gaatatcctt ttgattttaa 540

caaagagcat tgattatttt aagcttctag atgtgaaata aacggtcaac cac 593

<210>631

<211>1137

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(1128)

＜223〉n=a, c, g or t/u

<400>631

agtaaattta aaacagatat tgtcgaaaaa attatgagca aagattaagg cacgagaatt 60

tcaaaaaatt atttcccatt attactattg ataaaattga ctagcttatt atttactatt 120

gaactggtta tatgcattat gtgataatag gctggcatta ataatatctg tccattagaa 180

aaactgttaa gtgctcctgt tagcgatccg atttttagct aggtttaaag tataggtaac 240

tctgtcgcct gattcctcct cggcacatcg gtcctacatc cgacaatatt ttacggagtg 300

tttcataaca taacacggat atattgatta acaaatatta taaatagttt ctgtgttaaa 360

taagagttgt acaacatcta cagttttgca tcgagctgac ctagtttaaa aaaggatcgt 420

taacaagaga tgtaaagacg cagatatact cgactatcgt ccagatattt gtatatacag 480

cgtcttaaga tgtatcattt aacgaaatat acagttttta caagaagaaa agataatata 540

gtatggtaac cttatcatca aattaacatc gcataaaaac cgttcaagaa acacagagat 600

attgaggaaa agaaaagcac aacattaatt taatcaaaaa tatttctgtt ttacaatata 660

tctcactaag aaacatttct gatttataat attttagata tgtttagagg gagttataga 720

ccagggcgca tctgtaaaaa tattagtaca tttggacgtt gagaggtgac tcaaatttct 780

ttgcagaaat tgcacgaaaa caactcaaac aacaataatt gagtcaatca tcccactcaa 840

aaaggtccgg gaacattgcc taattaatca aaatgtcaaa aactgaagga aaatttcgat 900

tttttccttg gtcttttgat tataacttta aaactattca tttctgagaa aagttgtacc 960

gacatagaag ttgcgtaact aaatttcaca cattatagaa ttggtcaaaa attcaaaaaa 1020

tagtcacccc tgttgcaaaa cagcaataat tgcgaaaaaa ccatacaaaa acaagtattc 1080

gcattttacg cttttcaacc atttatgcta cacttaggac ccttttcngg acgcgtg 1137

<210>632

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>632

gtgctcctgt tagcgatccg atttttag 28

<210>633

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>633

gcaacttcta tgtcggtaca acttttctca g 31

<210>634

<211>781

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(100)

＜223〉n=a, c, g or t/u

<400>634

gtgctctgtt agcgatccga tttttagcta ggtttaaagt ataggtaact ctgtcgcctg 60

attcctcctc ggcacatcgg tcctacatcc gacaatattn tacggagtgt ttcataacat 120

aacacggata tattgattaa caaatattat aaatagtttc tgtgttaaat aagagttgta 180

cacaacatct acagttttgc atcgagctga cctagtttaa aaaaggatcg ttaacaagag 240

atgtaaagac gcagatatac tagactatcg tccagatatt tgtatataca gcgtcttaag 300

atgtatcatt taacgaaata tacagttttt acaagaagaa aagataatat agtatggtaa 360

ccttatcatc aaattaacat cgcataaaaa ccgttcaaga aacacagaga tattgaggaa 420

aagaaaagca caacattaat ttaatcaaaa atatttctgt tttacaatat atctcactaa 480

gaaacatttc tgatttataa tattttagat atgtttagag ggagttatag accagggcgc 540

atctgtaaaa atattagtac atttggacgt tgagaggtta ctcaaatttc cttgcagaaa 600

ttgcccgaaa acaactcaaa caacaatatt tgagttatcc tccctttcaa aaaggtccgg 660

aacattgttc aaacaatcaa aatgtcaaaa ataaaggaaa aactcgattt tttcttcgtc 720

tcttgactac aacttcaaaa gtattcattt ctgagaaaag ttgtaccgac atagaagttg 780

c 781

<210>635

<211>1142

<212>DNA

<213>Diabrotica virgifera

<400>635

tttattgtat taatgtataa tgtgcagtat cattcagacc tagtatttaa agaatttatt 60

ttaagttttt attcatacac acattttttt atgattatac tagttaatgc tgttaagtca 120

tagataagat aggtagatgt taagtcatgt ttgttctcat tttttgtaac ttcggcttgt 180

aatatttgtg gaagttaaaa agtagattgt ttcattttct attagtacaa aaatgttgaa 240

caacaattca atattttaca aaagtcaagt ggttttaatg tatctttggt ttatttaaca 300

aaaaaattta attggataga aattgcatat attctgaaaa ccatgctata actgccagat 360

ttgtgaacaa acaatagcaa gttcctaaga agaacttccg tagcagctat acaaatttaa 420

acctctgttt aaaaaatctg aattaagagt atatgatata ggcacataaa agtgatgtcg 480

aaattaggag tacaaaatgt taatttttaa taattaattt ggcatatgag actaactgaa 540

ggcaaacaaa acttaataac atcataaaat cattgcaata aaaaactgaa aactttttgt 600

cttccatgtt tttatgtggc gcagaaggta caaaaacgta aatctgtaat ttaaaacaat 660

tactgttttt ttatacgtag ttctggcaaa gttttacatt tgcattttag aaaaatgtgt 720

ttcatgttgt gtagtaaagc caaatttagt taactgtaaa aataaattac aaatttattt 780

tggttgtgaa ttggcccgaa aatttttttt atatctacat ttattcagtt ttgccaaact 840

actaacagat acgtattttc gaatatcctt ttgattttaa caaagagcat tgattatttt 900

aagcttctag atgtgaaata aacggtcaac cacaaaaaaa aaaaaaaagg gcggccgccc 960

tttttttttt tttttaacat aatagtgaaa tctttatttt aatacatatt atagtgacac 1020

attataaagc caatacatta acggcttata acgcatttgt cgttactcga tccatctact 1080

tgtgacagtt ggaagctgac gaagatattc gcagcttgtc cttcaagtgg gtcaatatca 1140

tt 1142

<210>636

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>636

ggaacgtaca caaaagtttg ggagggt 27

<210>637

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>637

gaacagcaac cagcctgata cgacg 25

<210>638

<211>582

<212>DNA

<213>Diabrotica virgifera

<400>638

ggaacgtaca caaaagtttg ggagggttta agggaacaaa acccccttaa aatttttatg 60

gggtggacaa atttcactat aattttgttt aaagatggtt ctgccatgag aatgatacat 120

gtccattttc aataaaaagt atctaatagt tttcgatata ttgaaaaaga tcgattttca 180

ttttgtaact tcaaggggct gtaactttta tgatgagcac atttgtacca aggtaagtta 240

gattcaatca aactattttt gaccccagaa tgtgtggtat aatttgtgac caatcttttc 300

gggacaccct gtatacaata tatgttccct atgtccagct gaacgattta cgtaggttag 360

atcgactaat atagtaaacg acaattataa tacgttttgt cgacaattat aatacgtttt 420

gtcgtcatat tttaaaagac tcacatttta atgactgacg ttaaatatac acacttctcc 480

atcaattcga taccatgatg ttgaaatacg atatcaaaat gtaaaagagg cacaaactga 540

atgcagacgt tcagtgacgt cgtatcaggc tggttgctgt tc 582

<210>639

<211>1039

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(748)..(886)

＜223〉n=a, c, g or t/u

<400>639

ccttcgcgtc cgtgataaca aaatcaatca aagagttgtt tactaaataa ttttgataat 60

atatttgaat aataataatt gatgtaaggc gtacaattaa taaaaagtta tttattgttt 120

atatattatt tatggaaaat ccaagtattt caagtcatta aagttcaaat aaaagtatta 180

ttttataaga gtttgttccg ataaccgtaa ataggtacga gtttagctat aaagtaaagt 240

gaccgtggtg gcataaaatg tagtaaatgc taatgcttca agcacgtact acatttttga 300

agatttcact acacttaaaa agcatatttg cttttctccg tagtatttgc tactatttac 360

cagcctacat agaagaatgt actacgcttt ttaaatatgt gcttgtttag tactatttgg 420

cttcagttta agtgaagttg gtgggtggac gttttcggcg taggtatttt tttgttacaa 480

tatggcagca tttatgaatg tatgtcatag aaaaatttac aaactacgaa gaagattctt 540

aatttttggg gtcattcttt tatcaaataa aatttaataa ttgtgaagaa gattaacaaa 600

taacaattgt ttatttttat ccttgcgtaa ctctttggtc gcttcggatc tgagtcactc 660

atttttactc atgtacaatt ctaataattt ggtacgtaaa atgtgaattc acaaatcaaa 720

agcacatgga aaactaattc catcacgnat ggtgtaatgc agacaccgct ggcgctacgc 780

tttgtagtag atacttctgg tcagtagtag gtactttctg tgtgtactaa gntctcaaca 840

aaagcaccta ctttttatgt aaaagaaaat gctacaaagt aatgactatt tactgnagca 900

ataacatcta ctacgtttat gcattccgcc catttcttga gtgaattatt aaatgtgact 960

tctattttat aaaaaaaatt ttagggtttt aaattaaagg ttccctttta ccgaaagttt 1020

taacaaattt ttttttttc 1039

<210>640

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>640

cgtccgtgat aacaaaatca atcaaagag 29

<210>641

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>641

ctactgacca gaagtatcta ctacaaagcg tagc 34

<210>642

<211>798

<212>DNA

<213>Diabrotica virgifera

<400>642

cgtccgtgat aacaaaatca atcaaagagt tgtttattaa atagtattga tagtgtattt 60

gaaaaataat aatttattta aggcgtacaa ttaataaaaa gttatttatt gtttatatat 120

tatttatgga aaatccaagt atttcaagtc attaaaattc aaataaaagt attattttat 180

aagagtttgt tccgataacc gtaaataggt atgagtttag ttataaagta aagtgcccat 240

ggtgtcataa aatgtagtaa atgctaatgc ttcaagtacg tactacattt ttgaagattt 300

cactgcactt aaaaagcatt tgcctttctc cgcagtattt gctactattt accagcctac 360

atagaagaat gtactacgct tttgaagtat atgcttgttt agtagtattt tgcttcagtt 420

taagtgaagt tggtgggtgg tcgtcttcgg cgtaggtatt tttttgttac aatatggcag 480

catttatgaa tgtgtgtcat agaaaaattt acaaactaag aagaagattc ttaatttttg 540

gggtcattct tttatcaaat aaaatttaat aattgttaag aagattaaca aataacaata 600

attgttcatt tttatcctcg cgtaactctt tgtcgcttcg gatctgagtc actcattttt 660

actcatgtac aattctaata atttggtacg taaaaatgtg aatttacaaa tcacaagcac 720

atggaaaact aattccatca cgattggtgt aatgcagaca cggctggcac tacgctttgt 780

agtagatact tctggtca 798

<210>643

<211>1035

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(790)..(857)

＜223〉n=a, c, g or t/u

<400>643

ctaataactg acaaaataac gcaaaagatg gaaaacatat aagttgtgag ataaggggaa 60

aagagactgg tagagatgct aaatttagtg ataaaagcct atgaacttgc attctacttg 120

gtgtttccca cctttagaca tatcagagct atatgtcaac taaaactgtc actgtcacag 180

tgtcagttaa caaacaagtc caatatgtct aaaggtggga aacagcagat agaatgtgga 240

tttataggtt ttatcactgg atttcgcacc tctactagtt taattccttt ttatctcaca 300

acttaatatg ttattagcat gctcatcact gtatactgac taactttttt tgttttttaa 360

cctcaccaaa ccttttttat tcgtatctgt atatttatcg aagttcaaat gtattttttt 420

tattcttcca aatgagccag aaacttgaca aaaaaacaat tttctgaatc ccttcgaata 480

aaattctttt taagtttatc tcatcggaat aaaaaatacc ttttctctct tggtaatttt 540

tcgaaaaatg cttccttttc acgttgtttg ctattttttg ttaaagaaat gccttaatta 600

gcgatttttg ggattttttc ccaaaaacta ctcaatgaat tataattcta caaaaggctt 660

tataatcctt aaaccctcaa gtataagtaa gaatattttg acaaggacac atggtttata 720

tctcgctgaa aacatggagt caaaaattca tctaaaatag gcccttcaga cagtctaccg 780

ctgccatgtn gcctaagtgn tggacagtgg gttgcagaaa aacgggcaca tgtcacataa 840

atatttgtat aaatttnaaa ctatcgtttg agcaagatag aagccactta tccttgtcaa 900

atattaataa taaggtttta agatataaag tttttatata ctacatttca tttagttgtt 960

tcttagaaaa aaatcgcaat aactcaaaat tcgtccctga aaatttttac taaatgattc 1020

cgaaaaatgt ttttt 1035

<210>644

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>644

gttgtgagat aaggggaaaa gagactggta g 31

<210>645

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>645

gacaaggata agtggcttct atcttgctca 30

<210>646

<211>853

<212>DNA

<213>Diabrotica virgifera

<400> 646

gttgtgagat aaggggaaaa gagactggta gggatgctaa atttagtgat aaaaacctat 60

aaacttgcat tctacttaat gtttcccacc tttagacata tcagagctat atgtcaacta 120

aaactgtcac tgtcacagtg tcagttaaca aacaagtcca atatgtctaa aggtgggaaa 180

caacagatag aatgtgaatt tataggtttt atcactaaat ttcgcacctc tactagttta 240

attccttttt atctcacaac ttaatatgtt attagcatgc tcatcactgt atactgacta 300

attttttttg ttttttagcc tcaccaaact ttttttattc gtatctgtat atttatcgaa 360

gttcaaatgt atttttttta ttcttccaag tgagccagaa acttgacaaa aaaacaattt 420

tctgaatccc ttcgaataaa attcttttta agtttatctc atcggaataa ataatacctt 480

ttctctcttg gtaatttttc gaaaaatgct tccttttcac gttgtttgct attttttgtt 540

aaagaaatgc cttaattagc gatttttggg attttttccc aaaaactact caatgaattg 600

taattctaca aaaagcttta taatccttaa accctcaagt ataagtaaga atattttgac 660

agggacacat ggtttatatc tcgctgaaaa catggagtca aaaattcatc taaaataggc 720

ccttcagaca gtctaccgct gccatgtgcc taagtgtgga cagtggttgc agaaaaacgg 780

gcacatgtca cataaatatt tgtataaatt taaaactatc gtttgagcaa gatagaagcc 840

acttatcctt gtc 853

<210>647

<211>1017

<212>DNA

<213>Diabrotica virgifera

<400>647

cgaactattt ttggtcccag aatgtgtgat ttagtttgtg acctgtattt ttgttacacc 60

ctgtatatga gatacaaatg aatcgcgatg attttttcta actagcgatt tcggtatatg 120

aatctgaatc ctgacagatg taaacataga ctaaataaac tacactcaga gaaaaaaatg 180

atgtgaaata aaattattcg gaggtagata agtgaaaaaa tacgaacgaa cagtaggaaa 240

ttaaaggcct tggggaaatg tggataaggt ctgaaatgac aggctgcgtt ctgttgatag 300

gtttgctctt atttaaaaaa tgtgaaaaat tcattattac atcagcaatt tatccctttc 360

ttgagtttat ggaacttatc agtttttcta aattaagcgt ttcaaatcaa attttttagg 420

aagtgttttt aaagggacga ttattcttta gtttttaaaa tgcgttttat ccaatgttgc 480

ctacttctta aatgtatata acaataaaag caataccatt ttggatgtta cataggttgt 540

taataaacag atgaaaaggt gtgtttgtct aagaacaggt atatatttgc aatgatatag 600

agattactgt agttaaagaa actatgcaaa agattagagg tgatagtgga tacagttgat 660

agctaataaa tcaataattc tatacattta tcttcgatta aataattatg ttattgtggt 720

tttcatggaa actattaaac tattttaatt aatgacgaag gtagaatacc ttaaaacttc 780

tccatggctc tgtcagaaga tatgaagtgt tcatttatta ataagtttcc tagttggaag 840

ggatttctaa ctttgtaatt ataaaaaatt ttttaaaaaa tttcttctta tttatctgga 900

gggttttttg aatccaccgc tggtaccatt attttttaac aaataaatgg taaaaacaaa 960

aattttatgg gaaaattttc ttacgggggg ggctttcaca ccaggaaatt tgttttt 1017

<210>648

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>648

ggtcccagaa tgtgtgattt agtttgtg 28

<210>649

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>649

gaacacttca tatcttctga cagagccatg 30

<210>650

<211>806

<212>DNA

<213>Diabrotica virgifera

<400>650

gtcccagaat gtgtgattta gtttgtgacc tgtatttttg ttacaccctg tatatgagat 60

acaaatgaat cgcgatgatt ttttctaact agcgatttcg gtatatgaat ctgaatcctg 120

acatatgtaa acatagacta aataaactac actcagagaa aaaaatgatg tgaaataaaa 180

ttattcggag gtagataagt gaaaaaatac gaacgaacag taggaaatta aaggccttgg 240

ggaaatgtgg ataaggtctg aaatgacagg ctgcgctctg ttgataggtt tgctcttatt 300

taaaaaatgt gaaaaattca ttattacatc agcaatttat ctctgggccc tttcttgagt 360

ttatggaact tatcagtttt tctaaattaa gcgtttcaaa tcaaattttt taggaagtgt 420

ttttaaaggg acgattattc tttagttttt aaaatgcgtt ttatccaatg ttgcctactt 480

cttaaatgta tataacaata aaagcaatac cattttggat gttacatagg ttgttaataa 540

acagatgaaa aggcgtgttt gtctaagaac aggtatatat ttgcaatgat atagagatta 600

ctgtagttaa agaaactatg caaaagatta gaggtgatag tggatacagt tgatagctaa 660

taaatcaata attctataca tttatcttcg attaaataat tatgttattg tggtttcaat 720

ggaaactatt aaactatttt aattaatgac gaaggtagaa taccttaaaa cttctccatg 780

gctctgtcag aagatatgaa gtgttc 806

<210>651

<211>1013

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(657)

＜223〉n=a, c, g or t/u

<400>651

cgaccgcaca aaaaaattgt taaaaggaaa ttgtaatata ttgtaaatgc gatttatttg 60

gaacaatttc agttcctaac atttttgtca caaagttaaa aatggcggag atattgaaca 120

aaaacaactg ctagacgacc aaaaaaatga aatagataaa aattatataa aatttaattg 180

tctccatttt tgtataggta catttttgtc gtaaaactaa taataaacgc gataactcaa 240

taattctgct gttacttttt tcccccatta ctcggaaaat atcgaccgca caaaaaattt 300

gtaataaaag acattataga aaatcatatt ttcaacaatt tcagtcgtcg cactttttgt 360

cgaaaagttg aaaatgacgg agatattgag caaaaacggc tctcctttaa attcaacatg 420

gcggctaacg taacggcgga attcactcgc gatattaaat ttacactact attgacaccc 480

gtaaagatta gaaaaattaa attttggaca tctcggcatg caaggtcaaa gctaaagtcc 540

tgtattttgg ctatctgtag gaaaacatca aaataactta ttataatgct gtttaatgac 600

atttttgagc cccttatgtt gaaacattat tttttttaat tgatactttc tgataanata 660

tacaaattta ttaaaataaa caccaaatca ctgtgaaatt gctacaaatg atatttaaaa 720

cattttttat gttgcgcaga agctatgcac caagtttttt ggatccaaat tgagtggttt 780

gaaatgaatc gcccataaat atttgagttt attgtcattg gaaactataa gcaagtatta 840

caatacgctt aatttttaat tttaaatagg gatttggact gactatgaat ctgatcaata 900

ttttttaaat aattgtaaaa tttgatgagt cattatccct gtgtgggtct gattgttttt 960

aatagaaact ttttttaaac agtgtaatat ttctagtaaa ataattttca acc 1013

<210>652

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>652

gatattgaac aaaaacaact gctagacgac c 31

<210>653

<211>29

<212>DNA

<213>Diabrotica virgifera

<400>653

gatcagattc atagtcagtc caaatccct 29

<210>654

<211>789

<212>DNA

<213>Diabrotica virgifera

<400>654

gatattgaac aaaaacaact gctagacgac caaaaaaatg aaatagataa aaattatata 60

aaatttaatt gtctccattt ttgtataggt acatttttgt cgtaaaacta ataataaacg 120

cgataactca ataattctgc tgttactttt ttcccccatt actcggaaaa tatcgaccgc 180

acaaaaaatt tgtaataaaa gacattatag aaaatcatat tttcaacaat ttcagtcgtc 240

gcactttttg tcgaaaagtt gaaaatgacg gagatattga gcaaaaacgg ctctccttta 300

aattcaacat ggcggctaac gtaacggcgg aattcactcg cgatattaaa tttacactac 360

tattgacacc cgtaaagatt agaaaaatta aattttggac atctcggcat gcaaggtcaa 420

agctaaagtc ctgtattttg gctatctgta ggaaaacatc aaaataactt attataatgc 480

tgtttaatga catttttgag ccccttatgt tgaaacatta ttttttttaa ttgatacttt 540

ctgataaaat atacaaattt attaaaataa acaccaaatc actgtgaaat tgctacaaat 600

gatatttaaa acatttttta tgttgcgcag aagctatgca ccaagttttt tggatccaaa 660

attgagtggt ttgaaatgaa atcgcccata aatatttgag tttattgtca ttggaaacta 720

taagcaagta ttacaatacg cttaattttt aattttaaat agggatttgg actgactatg 780

aatctgatc 789

<210>655

<211>1012

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(929)..(949)

＜223〉n=a, c, g or t/u

<400>655

catatttctt taataataat caattgctga acagataatg cacacaaata taatgtacac 60

tttcaacagt gcaatgtaac acacaagtcg cctgctgaat cgcaaaataa attatgttat 120

ctaatgtatt gtttgaaaga ccactaagta ccgattcggc attccagatt gtaaggaaaa 180

atgcaataag tgcaataatg agttttacca tgaaaccgaa attcacaatc ttggaaggaa 240

aaattcacta agtgcaataa tacgttttaa tgagttttgc cctgaaacca aaattcacaa 300

taatgcactt agtgaattgt accataaatt atctcagcaa ttagaagggt tagagtccca 360

ggtgacttct ccagagctgg gcagtaccgt atcgagttca aattaataaa aatgtcaata 420

tctcaaaaaa gtgcacttag ttagttttac ctctaatagg acgatttgtc ttgttttatc 480

cattatgata cattcacttc attatgtaca agcttatcga tatttgggta agtgtcgact 540

aggcagagtc agttcaatta ctatttcttc cttgagtgtt atgcttccat catcagccaa 600

gttaatgttg tataagtatc atactccata tcatagacaa atattcttca gtcacaaaat 660

atggctcagt aatagaagaa gagaagatag aaggactgga tggcagagaa cttgagaaag 720

tgaaatacac actacaaaaa ctttcaatgc cagtcttttg agatacatat ttggtcatta 780

ggtgatattc agattttgcg atattacata ttgtaggact ggtatgggag gagattctac 840

aggcaaaaga ccactcggac gtcccagaat gtgatggaga gatacatcca agcagatctc 900

cgaaaaatga acatcccatt tgaccctang ntgatggaag accaacaant tggaagaaag 960

tgtacagtca gccaggaccc accccagggt gtagcgctac gtgatgatga tg 1012

<210>656

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>656

tcaacagtgc aatgtaacac acaagtcg 28

<210>657

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>657

tgtatctctc catcacattc tgggacg 27

<210>658

<211>825

<212>DNA

<213>Diabrotica virgifera

<400>658

tcaacagtgc aatgtaacac acaagtcgcc ttctgaatcg caaaataaat tatgttatcc 60

aatgtattgt ttgaaagacc actaagtacc gattcggcat tccagattgt aaggaaaaat 120

gcaataagtg caataatgag ttttaccatg aaaccgaaat tcacaatctt ggaaggaaaa 180

attcactaag tgcaataata cgttttaatg agttttgccc tgaaaccaaa attcacaata 240

atgcacttag tgaattgtac cataaattat ctcagcaatt agaagggtta gagtcccagg 300

tgacttctcc agagctgggc agtaccgtat cgagttcaaa ttaataaaaa tgtcaatatc 360

tcaaaaaagt gcacttagtt agttttacct ctaataggac gatttgtctt gttttatcca 420

ttatgataca ttcacttcat tatgtacaag cttatcgata tttgggtaag tgtcgactag 480

gcagagtcag ttcaattact atttcttcct tgagtgttat gcttccatca tcagccaagt 540

taatgttgta taagtatcat actccatatc atagacaaat attcttcagt cacaaaatat 600

ggctcagtaa tagaagaaga gaagatagaa ggactggatg gcagagaact tgagaaagtg 660

aaatacacac tacaaaaact ttcaatgcca gtcttttgag atacatattt ggtcattagg 720

tgatattcag attttgcgat attacatatt gtaggactgg tatgggagga gattcctaca 780

ggcaaaagac cactcggacg tcccagaatg tgatggagag ataca 825

<210>659

<211>990

<212>DNA

<213>Diabrotica virgifera

<400>659

atgatacaat ttactattca tgcttcattc attcatgcta ttctttagag cattccacag 60

tgatctgtta aaatctagac gtcacacgaa gaactggaat tttccaagag tatcgaacaa 120

caaaatatcc aaatgtaacc tcaaaatagt atcattcgct tatacaaaac aatgctataa 180

tatgatcctt gctacataaa ctcactaatc acagttaagg ggggaggggt atggtttgac 240

atcagcattt cgagcacatt tttgtgaatt ttttgaatct atattagaat tatttttaaa 300

ttaaataaat agattcagta gagataggcg ttacctcacg aaatatctaa agaaagtctg 360

caaaatttca ggtggatcgg taaaatagtt ttggagttac aatatccacc acctttgaaa 420

aaagcagttt tgagaaaaat gcgttcaaag ttttgaaaac tttattttca attttatttt 480

tgtttgtcaa atcgtaaagt aatgcacacc gaaatatgtt tttgcatcgc ggagtaattt 540

acaaaagggg aacagctgtt gaacgtttct aactacgctc aagcacgctg tagcgaagct 600

gccgcaaagc gagtcgaagg tagggatatt tacttgctgt atctctggta attttactcc 660

aatcaatctg aaattttcgg agagtagtct tgaagttaat tttaaaattt caaaccgttt 720

caacctcccc ccttaatcac aaaaccttca actgtgttat tcacttcact tcaaatcgag 780

ttatacctat aacactctac tggaaagatt catactctat gtgttgtcac gttctagtat 840

ttcataaata ttattaaaag cgcttaaaat ttcagctaga tggacgaaat ttactttttt 900

agatccctag tttttcaaat tttacgtgct atattttttc tgcgtacgat atacctcctc 960

gcacattata ggtactggag ggaaaggtta 990

<210>660

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>660

agagcattcc acagtgatct gttaaaatct ag 32

<210>661

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>661

cgatttgaag tgaagtgaat aacacagttg a 31

<210>662

<211>688

<212>DNA

<213>Diabrotica virgifera

<400>662

agagcattcc acagtgatct gttaaaatct agatgtcaca cgaagaactg gaattttcca 60

agagtatcga acaacaaaat atccaaatgt aacctcaaaa tagtatcatt cgcttataca 120

gaacaatgct ataatatgat ccttgctaca taaactcact aattacagtt aaggggggaa 180

gggtatggtt tgaaatcaac atttcgagcc catttttgtg aattatttag agttagtgtt 240

acctcacgaa atatctaaag aaagtctgtg caaaatttca ggtgratcgg taaaatagtt 300

ttggagttac aatatcyacc acctttgaaa aaagcagttt tgagaaaaat gckttcaaag 360

ttttgaaaac tttattttca attttatttt tgtytgtyaa atcgtaaagt ratgcacacc 420

gaaatatgtt tttgmatcgc ggagtaattt acaaaagggg aacagctgtt gaacgtttct 480

aactacgctc aagcacgctg tagcgaagct gccgcaawgc gagtcgaagg tagggatatt 540

yacttrctkt atctctggta attttactcc aatcaatctg aaattttcrg agagtagtct 600

traagttaat tttaaaattt caaaccgttt caacctcccc ccttaatcac aaaascttca 660

actgtgttat tcacttcact tcaaatcg 688

<210>663

<211>997

<212>DNA

<213>Diabrotica virgifera

<400>663

caaatctgta atgtcaatac cgcaactgtc aaataaatgt taccaattta tgccaattta 60

tacctttgtt caatcgcagt tagagtgtaa tccgaacaag tgtgctttat aaaaacgctt 120

tatattccac ttatacagat taaacgaaac aagttagggt atatttcttt atatttaaat 180

taatagaaat ctttcaatat tatttctacg cgtatataat aaaatatgtc tagtggctgt 240

aattccagtg taccctgcta aacgattcta aaaaggaaca cacctacgac ttaaataatc 300

gtgtgacgtc acgtgctata acgcggatga cgtgcaacgg tgtctatatt gtacggactg 360

tattgaatgt gttaagtgtg tttaatattt aatattccga taactaatcg aataaaggat 420

tacagattac ggtaaatatt tattaaacat atatgattac aaacttttgt acatgtttta 480

ttttagaccg tgacaagtgc tctgatctga ttgacgttga caatgtgaca aatacactga 540

cacctaaagg tgtgttcaca ctatacacta aacaaagtgt tcaccaggtt tcgcgtggta 600

aattgaaaac aaaacgtgac tttctgttat gcaagccata tttttgttga attcaacatt 660

ttattggtca ccatttatga ttagttaact agtaatttgt tatgttgaat ttggttatgc 720

gatatccgat aagaacttag cctctccgcc ctagtgcgtc actattgcaa gggatatata 780

ccatcgtata atacattctt gtagaccgcc tatgtcaaaa tctgagccaa atcgggactt 840

gtaatttttt taatttatta agaaaaacat acaatcccca tccacccccg cagggttatt 900

aaaaggagta gtaacgtacc caaaaattcc gttttaataa taataaaaaa gagggaatat 960

aaaaagtaaa atatttttaa aaaaatttaa acccctt 997

<210>664

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>664

caatcgcagt tagagtgtaa tccgaaca 28

<210>665

<211>31

<212>DNA

<213>Diabrotica virgifera

<400>665

gtacgttact actcctttta ataaccctgc g 31

<210>666

<211>848

<212>DNA

<213>Diabrotica virgifera

<400>666

caatcgcagt tagagtgtaa tccgaacaag tgtgctttat aaaaacgctt tatattccac 60

ttatacaaat taaacgaaac aagttagggt atatttcttt atatttaaat taatagaaat 120

ctttcaatat tatttctacg cgtatataat aaaatatgtc tagtggctgt aattccagtg 180

taccctgcta aacgattcta aaaaggaaca cacctacgac ttaaataatc gtgtgacgtc 240

acgtgctata acgcggatga cgtgcaacgg tgtctatatt gtacggactg tattgaatgt 300

gttaagtgtg tttaatattt aatattccga taactaatcg aataaaggat tacagattac 360

ggtaaatatt tattaaacat atatgattac aaacttttgt acatgtttta ttttagaccg 420

tgacaagtgc tctgatctga ttgacgttga caatgtgaca aatacactga cacctaaagg 480

tgtgttcaca ctatacacta aacaaagtgt tcaccaggtt tcgcgtggta aattgaaaac 540

aaaacgtgac tttctgttat gcaagccata tttttgttga attcaacatt ttattggtca 600

acatttatga ttagttaact agtaatttgt tatgttgaat ttggttatgc gatatccgat 660

aagaacttag cctctccgcc ctagtgcgtc actattgcaa gggaaatata ccatcgtata 720

atacattctt gtagaccgcc tatgtcaaaa tctgagccaa atcggacttg taattttttt 780

aatttattta gaaaaacata caatccacat caaccacgca gggttattaa aaggagtagt 840

aaccgtac 848

<210>667

<211>996

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(744)

＜223〉n=a, c, g or t/u

<400>667

atttaatcag tcgtaaaaaa atatacagtt cctctaattg gcttagctca cgctgggtta 60

agctgatttc aatagccatt agactaatag tatttatgaa tgactgttta attgacttca 120

aacgatttcc ataaacttaa ttacaattta cgccgttatt aatcaaaact catagttggc 180

gcaacgattt gaaattgttc taatttcgag gcgaatcatg taaattttat tgcgtttgag 240

tgacattgta ttttccataa tatgcgtgcg gtgtccttta cattgcaaac tatttgtatt 300

tgagtagtat cgttaaattt cgagaggtaa aatattgaat agctggtata ataattaaaa 360

gtacaataga ttaacaatac tttctacctg taccggaaac ttaccacacc attcaaatac 420

aaatgactgt aagcttaaaa tttttctgga atattttaat ggcaactata agtagtttcc 480

gcggtgaaac accttgtatg catttaaatg gtttcggctc acgtcagcaa tgaaagatgc 540

tcattctttt cagtataaaa tccaaaacta atttgagact gaatcgagtt gggtgataaa 600

gttgtaatac ggtgcgatat ggagtctggc gtaagatttt ttgtacacgc tgtatcgaca 660

caaattgtgc agtatttaat gtaagatcat ccattcttcc gtatcttcca aaacttttta 720

aatatactac gtttaaaact ctanagttga ttttagtttc cccaaaacag caatactcgc 780

ggtttagttg agttgaaagt gtgatagaca ggttttagtt actatataaa tatttttttt 840

acgatttgta ttagttgtaa tatacaatga aagacggaat tgtttttcgt tgtgtataaa 900

actttgtgtg actttttgaa atgtacatag ttctagtata agtttgttgt gctgagccac 960

gagaagtatc tcggttggaa ttgttccttt tttttt 996

<210>668

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>668

acagttcctc taattggctt agctcacg 28

<210>669

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>669

cttctcgtgg ctcagcacaa caaac 25

<210>670

<211>949

<212>DNA

<213>Diabrotica virgifera

<400>670

acagttcctc taattggctt agctcacgct gggttaagct gatttcaata gccattagac 60

taatagtatt tatgaatgac tgtttaattg acttcaaacg atttccataa actttaatta 120

caatttacgc cgttattaat caaaactcat agttggcgca acgatttgaa attgttctaa 180

tttcgaggcg aatcatgtaa attttgttgc atttgggtga cattatattt tccataatat 240

gcgtgcagtg tcctttacat tgcaaactat ttgtatttga gtagtatcgt taaatttcga 300

gaggtaaaaa tattgaatag ctggtataat aattaaaagt acaatagatt aacaatactt 360

tctacctgta ccggaaactt accacaccat tcaaatacaa atgactgtaa taagcttaaa 420

atttttttct ggaatatttt aatggcaact ataagtagtt tccgcggtga aacaccttgt 480

atgcatttaa atggtttcgg ctcacgtcag caatgaaaga tgctcattct tttcagtata 540

aaatccaaaa ctaatttgag actgaatcga gttgggtgat aaagttgtaa tacggtgcga 600

tatggagtct ggcgtaagat tttttgtaca cgctgtatcg acacaaattg tgcagtattt 660

aatgtaagat catccattct tccgtatctt ccaaaacttt ttaaatatac tacgtttaaa 720

actctaaagt tgattttagt ttccccaaaa cagcaatact cgcggtttag ttgagttgaa 780

gttgtgatag acaggtttta gttactatat aaatattttt tttacgattt gtattagttg 840

taatatacaa tgaaagacgg aattgttttc cgttgtgtat aaaacttgtg ttgacttttt 900

gaaatgtaca tagttctagt ataagtttgt tgtgctgagc cacgagaag 949

<210>671

<211>991

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(906)..(921)

＜223〉n=a, c, g or t/u

<400>671

attcgaactt cccagaagat tgaatggata aatgcaggta ctcaaatgtt tgcaacatgt 60

tctatcgatg cttcttgaag acgtagcttt attgtaataa aaaaatttta tgtaagttga 120

aacactatac aaaccttatt tattagatat ttaataaaca caaacattat gctgtttttc 180

tattactgtt atcagtattt tatcaatgta tattatgcct atgatcgatg attcatgata 240

gttgagttac agtaaaatta ttttagcaca gaaggaacgc agcgctgccg gctgtatttg 300

cttttctgtg gacattaatc aaatgcatta aaattaataa attatttttt tgaaaacggt 360

tgacttaaca aaaaaaattt attagacttt tttgctctaa atggtgcact tagcccatac 420

cttggaggaa cgcactattt tccgggacac cctgtatagt ttaaaaatta ttttctgttg 480

ctcaatcggt cattcgttcg tggaagtaga tacatcatgc attaattgaa agatctatcc 540

actggtggtt gtaacttaac gtccgcatca ttctgcgttt aattcaggtt ccaaaactga 600

catttaagtc caatattttt tgttcacgtt tggtacaaat attttttaga agggtatgcg 660

tccaagttct tacgagcttt gctgttgctg cctgctccta cgtcaaatgc tgaatttgca 720

tgaacactga accttacttt ccaaggaata tattaaataa tatcatttat ttaatacttt 780

tcgtagcaag ctaagtaatg ttaacgaaga gaattatgac tttgcttagc aaatacagag 840

tttttatttt atagccttga atttttgtta attttgatat ctatacgtat atattgtttt 900

atattngtac atgtactttg ngtaatagct gccgatattg ccatttttta taaaaataac 960

gagagcagag gtgtataaat aaatttttgg t 991

<210>672

<211>28

<212>DNA

<213>Diabrotica virgifera

<400>672

cttcccagaa gattgaatgg ataaatgc 28

<210>673

<211>30

<212>DNA

<213>Diabrotica virgifera

<400>673

gaaagtaagg ttcagtgttc atgcaaattc 30

<210>674

<211>733

<212>DNA

<213>Diabrotica virgifera

<400>674

cttcccagaa gattgaatgg ataaatgcag gtactcaaat gtttgcaaca tgttctatcg 60

atgcttcttg aagacgtagc tttattgtaa taaaaaaatt ttatgtaagt tgaaacacta 120

tacaaacctt atttattaga tatttaataa acacaaacat tatgctgttt ttctattact 180

gttatcagta ttttatcaat gtatattatg cctatgatcg atgattcatg atagttgagt 240

tacagtaaaa ttattttagc acagaaggaa cgcagcgctg ccggctgtat ttgcttttct 300

gtggacatta atcaaatgca ttaaaattaa taaattattt ttttgaaaac ggttgactta 360

acaaaaaagt ttattagact tttttgctct aaatggtgca cttagcccat accttggagg 420

ggcgcactgt tttcagggac accctgtata gtttaaaaat tattttctgt tgctcaatcg 480

gtcattcgtt cgtggaagta gatacatcat gcattaattg aaagatctat ccactggtgg 540

ttgtaactta acgtccgcat cattctgcgt ttaattcagg ttccaaaact gacatttaag 600

tccaatattt tttgttcacg tttggtacaa atatttttta gaagggtatg cgtccaagtt 660

cttacgagct ttgctgttgc tgcctgctcc tacgtcaaat gctgaatttg catgaacact 720

gaaccttact ttc 733

<210>675

<211>990

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(825)..(935)

＜223〉n=a, c, g or t/u

<400>675

aacgaatgcc aaacctatga gggaacttac acattttttt attacgtaat attattcagt 60

ttcctttaaa aataatattt ccaaactttc acgcggtaaa acctcataat aacttagaag 120

ataatttaaa atctatatat ttttttctgt tttctggcct tggtttagaa gaacccttag 180

ccaatgtaaa atagttcaaa ctatcaaaaa cgcttgtgac gttacattac tgtattttct 240

actgacgttt atagtgtcta atttaaaatt atatacaatt ttgtttactt tgttggtgca 300

gaatgtacat gcacaactat atgacgtcaa tatgcgtttt ggaccacctg ttttaatttg 360

aatttttaat cgtctttagg ggccaaacga aagacaaaca aaactttttt tatctttgat 420

acatgtttta aattatgttc tgttgtgatt tataacattt ttttcgaatt taaaaattta 480

tgcaagttcc ctatttacac aaacaattta atatttccaa caagagcatt atttaaaatt 540

ccttgttccc gctaagggat agggacttca catcctagca gggggcgtta agacgctaga 600

aaaccggaag ataagtatag ctatcacaga agatatcggg gaagaacagg tatgtacatc 660

ggttatgact ataaatatct gaaaaataca tacatacttg taaagtaaac accactctta 720

ccattttcta atattatcta agtattgact ggacttttct atctgcctca tgaataatga 780

ggtaaattag aatttcattt attttgcata aaaaatacat gtaanttccg ttatcataaa 840

ataattatag gaatatctac cgatattatt actctggggt ccgtatatct atcctttttt 900

tctacttttc tactatggnn atgagaaata aattnaaatt ttaaaaacaa tttggtggta 960

ttccctagag gaaatataaa attttatcta 990

<210>676

<211>32

<212>DNA

<213>Diabrotica virgifera

<400>676

agaagaaccc ttagccaatg taaaatagtt ca 32

<210>677

<211>33

<212>DNA

<213>Diabrotica virgifera

<400>677

gatagatata cggaccccag agtaataata tcg 33

<210>678

<211>729

<212>DNA

<213>Diabrotica virgifera

<400>678

agaagaaccc ttagccaatg taaaatagtt caaactatca aaaacgcttg tgacgttaca 60

ttactgtatt ttctactgac gtttatagtg tctaatttaa aattatatac aattttgttt 120

actttgttgg tgcagaatgt acatgcacaa ctatatgacg tcaatatgcg ttttggacca 180

cctgttttaa tttgaatttt taatcgtctt taggggccaa acgaaagaca aacaaaactt 240

tttttatctt tgatacatgt tttaaattat gttctgttgt gatttataac atttttttcg 300

aatttaaaaa tttatgcaag ttccctattt acacaaacaa tttaatattt ccaacaagag 360

cattatttaa aattccttgt tcccgctaag ggatagggac ttcacatcct agcagggggc 420

gttaagacgc tagaaaaccg gaagataagt atagctatca cagaagatat cggggaagaa 480

caggtatgta catcggttat gactataaat atctgaaaaa tacataatac atacttgtaa 540

agtaaacacc actcttacca ttttctaata ttatctaagt attgactgga cttttctatc 600

tgcctcatga ataatgaggt aaattagaat ttcatttatt ttgcataaaa aatacatgta 660

attccgttat cataaaataa ttataggaat atctaccgat attattactc tggggtccgt 720

atatctatc 729

<210>679

<211>988

<212>DNA

<213>Diabrotica virgifera

<400>679

cggacgcgtg ggtgttccgg accattttga gtgggaggat agctcaagtg ttgttgtttg 60

ggttattttc gggcaatttc tgcagggggg tttgagtcac ctctcaacgt ccatctcaaa 120

acagatgcgc cctggactaa aacaatttta agtactctca ttgttaaaaa gttgattcac 180

cacattttca taacgatttt tagggcggct gtacacggac tgctgaggtc gtttatcact 240

gagcgacata cacggactgc tcgagcgatc gttgtagact tctggagcgg tccattgttg 300

actacctgag ttgtcccttg tcgcattgca accgctctcg atctgtcgat agcacgatgg 360

aatttcatta tgctattgac agcttgaagc ggtcttgact gctcgagctg tcgtgtgtat 420

ggcaacgaat atacaattat aattcaccgc gcaaacgctg cttcaaaaag tcgacctcaa 480

ctgcttgaag atgtccgtgt acggccgatc ttggggtatc ttttttactc ttatcgggaa 540

tatttaagaa ccgaaatccc taatgttttc cctatttgtt ccagaatgat atacgtgtta 600

atttccaact gagacttgac ggccagcacg accgtacgac aagacatttt cgaacagtaa 660

aatcattagt tttagtaaca aatacaaaga cagatgggaa ttcggttctc gatattatgc 720

agagtattta cctgatgatg aacacttatt ggaggataga aacgcataat ttgcagaact 780

gacttaccca tgtaatggac aggtatgatg gaaacgtatg tactttaatg ttttgttagt 840

atttttacga gagaagctaa gtaggtttgt gaagaattag ttatttgttg tactcgatac 900

tttttattaa ctcattaatt aattaaatcc tacaaataca caacgtaaga agctacatga 960

gcgcgcagca aaaatttttt tttttttt 988

<210>680

<211>25

<212>DNA

<213>Diabrotica virgifera

<400>680

ggaccatttt gagtgggagg atagc 25

<210>681

<211>33

<212>DNA

<213>Diabrotica virgifera

<400>681

ctaattcttc acaaacctac ttagcttctc tcg 33

<210>682

<211>862

<212>DNA

<213>Diabrotica virgifera

<400>682

ggaccatttt gagtgggagg atagctcaag tgttgttgtt tgagttattt tcgggcaatt 60

tctgcaggga agtttgggtc acctctcaac gtccacctca aaacagatgc gccctggact 120

aaaacaattt taagtactct cattgttaaa aagttgattc accacatttt cataacgatt 180

tttagggcgg ctgtacacgg actgctgagg tcgtttatca ctgagcgaca tacacggact 240

gctcgagcga tcgttgtaga cttctggagc ggtccattgt tgactacctg agttgtccct 300

tgtcgcattg caaccgctct cgatctgtcg atagcacgat ggaatttcat tatgctattg 360

acagcttgaa gcggtcttga ctgctcgagc tgtcgtgtgt atggcaacga atatacaatt 420

ataattcacc gcgcaaacgc tgcttcaaaa agtcgacctc aactgcttga agatgtccgt 480

gtacggccga tcttggggta tcttttttac tcttatcggg aatatttaag aaccgaaatc 540

cctaatgttt tccctatttg ttccagaatg atatacgtgt taatttccaa ctgagacttg 600

acggccagca cgaccgtacg acaagacatt ttcgaacagt aaaatcatta gttttagtaa 660

caaatacaaa gacagatggg aattcggttc tcgatattat gcagagtatt tacctgatga 720

tgaacactta ttggaggata gaaacgcata atttgcagaa ctgacttacc catgtaatgg 780

acaggtatga tggaaacgta tgtactttaa tgttttgtta gtatttttac gagagaagct 840

aagtaagttt gtgaagaatt ag 862

<210>683

<211>978

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(876)

＜223〉n=a, c, g or t/u

<400>683

aaaagaaata gaacgtaata atgaaacaac aacaaaaaca acctctggaa cataagcatc 60

tactattaat ttgatgcagc taaggaaata atatgggcaa caagtaaaaa agcctcctca 120

aacaaacaga cagagtggtg gtgacaaaaa aagaggtggc atagataggt gtcttttagg 180

aaatatttgg aaaagaacga ccatacttat aataatacaa ctgtaaatgt gtaaaaaatc 240

gtattgattt tgctacttgt gtctattgtg tcattttatt agatcacaat atatataaac 300

tgttgaactc gaccgtaagc tgctttaaaa acaataaaat gttttgaaat gtagtaaata 360

tgtcatcgaa taagtgacgt catagctaag tgtagacagt attgccaaaa taatttgtat 420

acataatggt tcttctattt atacaaaact ttaatctttt taatttcttc tctttgtatt 480

tgactattaa caaagtgaaa cgaattttgc aattatttta gtatattaac gtagaaaaag 540

atcaataatt gactctagag acttttaatt cttaacaaaa ttaaacaatt atattaaaca 600

ccaaaactac tctgatcaag tcatggtctc tgcatattat ttatacctta gagctacact 660

atttagaaca aaatgttaat ataaacattt tttaataggt attttgagat agaaagtgta 720

tgtgaaaatt ctgtgtttat aatgcaaaac cccatacacg gcttaggaat aagtttatgt 780

aggctaaaca cagacgaaat tagttatatt tatggaaact gagactacga cgtagttcga 840

cgaaatataa acagtctacg ggatgtccta tcgtgntata gccgaatcaa atttaatata 900

agggtagtta tgactaaaaa attgaaaacc gtacatgatc atatcacagt ttatggcata 960

cattatggtc aacacatg 978

<210>684

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>684

agcctcctca aacaaacaga cagagtg 27

<210>685

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>685

gaccataatg tatgccataa actgtgatat gatc 34

<210>686

<211>857

<212>DNA

<213>Diabrotica virgifera

<400>686

agcctcctca aacaaacaga cagagtggtt gtgacaaaaa aagaggtggc atagataggt 60

gtcttttacg aaatttggaa aaggacgacc atacttataa tacagctgta aatgtgtaaa 120

aaatcgtatt cattttgcta tttgtgtcaa atgtgtcatt ttattggatc acaatatata 180

taaactgttg aactcaaccg taagctgctt taaaaacaat caaatgtttt gaaatgtagt 240

aaatatgtca tcgaataagt gacgtcatag ctaagcgtat acagtgttgc caaataattt 300

gtatacataa tggttcttct atttatacta aactttaatc tttttaattt cttctctttt 360

cgtatttgag tattaaccaa gtgaaacgaa ttttgcaatt attttagtgt attaacgtag 420

aaaaagacca ataattgact ctagagactt tcaattctta acaaaattag acaattatat 480

taaacaccaa aactaatctg atcaagtcat gatctcgctg catattattt ataccttaga 540

gctatactat ttagaacaaa atgttaatat aaacattttt aataggtatt ttgagataga 600

aagtgtatgt gaaattctgt gtttataatg caaaacccat tatacggctt aggaataagg 660

taaggtaggc taaacacaga cgaaattagt tatatttatg ggaactgaga ctacgacgta 720

gttcgacgaa atataaacag tctacgggat gtcatatcgt ggtatagccg aatcaaaatt 780

taatataagg gtagttatga ctaaaaaatt gaaaacgtta catgatcata tcacagttta 840

tggcatacat tatggtc 857

<210>687

<211>977

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(730)..(938)

＜223〉n=a, c, g or t/u

<400>687

tatgaataaa aatagcattt ttatatacaa taacctggag acgggtcaca ggcttcatgt 60

gcaacactta acaccttcag ctccaggcca atatctgata tttgccacat gtgtcacttt 120

tatacagtca ttggaaaatt gttgaattgt aaacacggta gccgtgtccg tgtcggtggc 180

acctgattga taagaggatg aacacgggag ccgtgtcggc agacttaaag tgttttgaga 240

aaaagtttgt tttaataatt aatatttcgt cctattagag gtaaaactca ctaactacac 300

ttttcagaaa gtggagaaaa atcgatttaa aggtacaaat tgttttttga attcacctga 360

cttgaatatt ggaatttttt aaatgcaact ttaaagaata aataaaaaca gatattttgt 420

gccatatttc gtcacaggaa ctatgataca atcaatatta gatcaatttt tgtacttaat 480

gagtttttcc atgaacaaaa ttattggata ttgatatcct atcagaggta aaactcacta 540

actacacttt gtacttgatg agtttttcct tgaacacaat tattggatat tgatatccta 600

tcagaggtaa aactctttaa ctacactttg tacttgatga gtttttcctt gaacaccatt 660

attggatatt gagaacttaa tgaatttgtc tccataaaat ttcgctgact tacaacaaac 720

gaactatcan aattaacagg caattatgtc acaggaacta tgatataatc atgatacaat 780

caatgttaga tcaatttttg tacttaatga gtttttccat gaacacaant attggatatt 840

gatatcctat cagaggtaaa actcgctaac tacactttgt attttgatga gtttttcctt 900

gaaacacatt attgaatatt gatatcccta tcagaggnta aaactcacta actacacttt 960

gtactttgat gagtttt 977

<210>688

<211>23

<212>DNA

<213>Diabrotica virgifera

<400>688

ggagacgggt cacaggcttc atg 23

<210>689

<211>34

<212>DNA

<213>Diabrotica virgifera

<400>689

caaagtgtag ttagcgagtt ttacctctga tagg 34

<210>690

<211>509

<212>DNA

<213>Diabrotica virgifera

<400>690

ggagacgggt cacaggcttc atgtgcaaca cttaacacct tcagctccag gccaatatct 60

gatatttgcc acatgtgtca cttttataca gtcattggaa aattgttgaa ttgtaaacac 120

ggtagccgtg tcggtggcac ctgattgata agagaatgaa cacgggagcc gtgtcggcag 180

acttgaaagt gttttgagga aaagtttgtt ttaataatca atatttcgtc ctattagagg 240

taacactcac taactacact tttcagaaag gggagaaaaa tcgatttaaa ggtacaaatt 300

gttctttaaa ttcacctgac ttgaatattg gaattttttt aatgcaactt aaaagaataa 360

ataaaaacag atatttgagc catagttcgt cacaggaact atgatacaat caatattaga 420

taaatttttg tacttaatga gtttttccat gaacacagtt attggatatt gatatcctat 480

cagaggtaaa actcgctaac tacactttg 509

<210>691

<211>962

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(836)..(933)

＜223〉n=a, c, g or t/u

<400>691

tgtcttttta gtttacttct cttggtttaa aaacaaagct tagctattta tgactgcaat 60

tttgatatcg tgtgtattag aaactttagt ttttaaccac gatatttaaa tattgtggac 120

tttgaacaat taaaaaaaaa tgtacttggt cactttctgt tggcgaaaaa aattagaaat 180

ttgacttggt caatataagc gacaccgtat taggaaatta tatttattaa ctacggtctg 240

tacatagaaa aaaattaaaa tctaatcagt ttctgtgtcg tcgctttcag aagaagctgg 300

ttacggtatg aaaacttttt ttgactactg gaatcgattt ataccactca tggaactctt 360

gcggaataat tttatttcta cgcaaggagg ctgtttttat tttgctctgg tatagacaaa 420

agttttttgt ataacattgg aatgttctta ggaataacag ctggtctgac cttgccacat 480

actcaagagt aacgcattta attttaacca cgatattttg tttccatttt aatcttagtc 540

tctgtttttt aagaacatct gtgcttaaga tcgaaaaagt ctgaccgtaa ttcttcaact 600

ctgtaggcac cgctcttttc gttgcgtctt gtagaacgga ctagcttgaa aatattaaca 660

gatcgttcat tgtaaaaaat ggcaacattt tcattaacag actatatggc actgtgcatt 720

gagtttaccg tttacctcca tgtacgaatg tcctttctcc ataaaattat gctgcaaaat 780

cttagtgtgt gcgttacaac aacatacaaa ttaatgcggc aatgaacgaa tataanaatc 840

tctgataagt cagaatataa actgatctct gtgatgcctt ttgatcatgt gattgcagan 900

gttttatccg ctttgatcat gctattccna ttncagagct tcccacgtga ccattaatct 960

ct 962

<210>692

<211>27

<212>DNA

<213>Diabrotica virgifera

<400>692

tgacttggtc aatataagcg acaccgt 27

<210>693

<211>35

<212>DNA

<213>Diabrotica virgifera

<400>693

gcatcacaga gatcagttta tattctgact tatca 35

<210>694

<211>697

<212>DNA

<213>Diabrotica virgifera

<400>694

tgacttggtc aatataagcg acaccgtatt aggaaattat atttattaac tacggtctgt 60

acatagaaaa aaattaaaat ctaatcagtt tctgtgtcgt cgctttcaga agaagctggt 120

tacggtatga aaactttttt tgactactgg aatcgattta taccactcat ggaactcttg 180

cggaataatt ttatttctac gcaaggaggc tgtttttatt ttgctctggt atagacaaaa 240

gttttttgta taacattgga atgttcttag gaataacagc tggtctgacc ttgccacata 300

ctcaagagta acgcatttaa ttttaaccac gatattttgt ttccatttta atcttagtct 360

ctgtttttta agaacatctg tgcttaagat cgaaaaagtc tgaccgtaat tcttcaactc 420

tgtaggcacc gctcttttcg ttgcgtcttg tagaacggac tagcttgaaa atattcaaca 480

gatcgttcat tgtaaaaaat ggcaacattt tcattaacag actatatggc actgtgcatt 540

gagtttaccg tttacctcca tgtacgaatg tcctttctcc ataaaattat gctgcaaaat 600

cttaatgtgt gtgttacaac aacatacaaa ttaatgcggc aatgaacgaa tataaaaatc 660

tctgataagt cagaatataa actgatctct gtgatgc 697

<210>695

<211>765

<212>DNA

<213>Diabrotica virgifera

<400>695

gttcaaaaca gaacaattac actacattta ttgattctaa caatctaata gatgttataa 60

gatactcgtt tatcttaatc tgtacaatta actttaattg ggtaatgtaa tatacaagat 120

taaaagggta aaagaaaaat ttaaaacaat ttacatgaag aaaaccaagt aatactgaaa 180

atataaacga tattaagcac ctgtatattt aaaaaagtat taaaacgaaa aaacttaata 240

gtaattggaa tggcgggaaa aatgtttaaa aagtatttaa taagcactgt accctcaccc 300

aaaaaaacta tacacatatt caacaattta cataatttat tttgtacagg ataatatcaa 360

aaaagagaaa acctatcttt agttactatc tttagtacgg ccatattaat attcacagaa 420

gtgagggata gaggccttat tattcttttt gataaatatc gctatatttt taaacttaca 480

ctgatgaaca aaataggaaa atttccttaa aaagatttaa tcttctaagt ttctgaaggc 540

ctgctgaata tcttcatata attgatcaaa atctgccttg attttagctt caccatcttt 600

tacgggatcc ttaaatttca tactgctaag ttgatataaa attccactca ttgaatctct 660

tattacgttc caagtgatct tattttctga ttgtgcggtt gattctacag cgtgtctcgc 720

catgtcgtac aaaccgatca tgtttctcaa cataccgaca gtttt 765

<210>696

<211>594

<212>DNA

<213>Diabrotica virgifera

<400>696

cttatttcga tttgactatg tgatggattg agaccttagg cagagcctta acgttgattg 60

ccgagtcaag acctgtaggc atctataaag gcgactatat acaagaatta tatgcaagat 120

atgatgatgt cagtgatgct ccacctccac catctcttcc tgattggtgt acagaaagtg 180

acgatggaca agggaatggc cttgacgatt tcgatgattt tactgaagat gcaagaaaac 240

gagtagcttc aacttcttca aataatgatg atacttcacc aaaagctaaa aagggtcgct 300

tctaggaagt cgcatttatg gagggagtac ctaacgttac ccactttgta gaggaacaag 360

cgacccgaga ttgcaaacga atggtgccta ggatgtgtag gtgtacacca aaatggtgtt 420

ccaggaagtc atgtccgtgt ccaatggata tgacaaacat tactcttcta aaagaccacc 480

cctacagagt ttcctgcaaa gcagacggcc atgaggcatt atgaagctta tcaacggaga 540

acacaatgtt tatctttttg acagagatga tgaagcttat taaggcggaa ggca 594

<210>697

<211>884

<212>DNA

<213>Diabrotica virgifera

<400>697

gcttgagtaa aaactgaaat catataaaag agataatttc tattgtgaac taatttttca 60

aaagtttcta cattaagtac atattcatta ctaaataatg gccgaaaaca aaaatccaga 120

ggatttggcc accgcaattc tccgtaagaa agatcgtcca aatagacttt tggtagaaga 180

agcagttaac gatgataact ccgtggttgc tttgtcacaa gcaaaaatgg atgaactaca 240

acttttcaga ggagacacag tcctactgaa gggcaagcga aggaaagaaa ctgtttgtat 300

agtattatct gatgatacat gtagcgatga aaaaattcgt atgaatagaa ataatttaag 360

agttaggctt tcagatgtag tttctatcca agcttgccct gatgtaaagt atggcaagag 420

aatacatgta cttcccatag atgatacagt ggaaggactc acaggaaatc tcttcgaagt 480

atacctgaaa ccctacttcc tggaggccta tcgaccaata cacaaagatg acgtattcgt 540

tgtacgtgga ggaatgagag ctgttgaatt caaagtcgtc gagaccgatc ccgctccgta 600

ctgtatcgtg gctccagaca ccgtcattca ttgtgatgga gatccaatca agcgcgaaga 660

ggaagaggaa gctcttaacg cagttggata cgacgaacat ccggaaggtt gtaaaaaaac 720

aggttgctca aatcaaagaa atgggtagag ttacccactg agacatccct agtctttttc 780

aggggtattg gaagtaaacc caccaaaagg tattttactt taccgggtcc accggggaac 840

tggtaaaacc cctccttgcc cccgcaagga acctaaacaa aact 884

<210>698

<211>642

<212>DNA

<213>Diabrotica virgifera

<400>698

gccctttttt tctgtccatt ttatgtcaca agtagggcta aaatgaagca aattgtaaca 60

aaccaaacgg taaaaattcc tgagggaatt acagtaacca ctaagtcaag ggtagtaact 120

gtaaaaggac cccgaggtaa tctgaagaga tccttcaaac atcttaccct ggacattcga 180

atgatcaacc ccaggttact gaaagtagag aaatggttcg gtaccaagaa ggaattagct 240

gctgtcagaa cagtatgctc tcatgttgaa aacatgttaa agggtgttac aaaaggatac 300

caatacaaga tgagagcagc atatgctcac tttcccatta actgtgtcac cactgaagga 360

aacacagtta tcgaaattag aaatttcttg ggagaaaaat acatcagaag agtaaagatg 420

gccccaggtg ttacagtagt aaactccacc aaacaaaagg atgaacttat cattgaagga 480

aactcattgg aggatgtttc aaagtcagct gcccttattc aacaatctac aacagttaaa 540

aataaggata tccgtaaatt cttagatggg ctttatgtat ctgaaaaaac aactgttgta 600

caagaagaat aacttgtatt tttgaaataa atttaagttt at 642

<210>699

<211>652

<212>DNA

<213>Diabrotica virgifera

<400>699

cacgttgaga ggtgcatttg cacgatgagt tccttaaaac ttcagaagag gctagcagcc 60

tctgttatgc gatgtggtaa aaagaaagta tggttggacc ctaatgaaat caacgaaatt 120

gccaacacta actcaagaca gaacatccgt aagttgataa aggatggtct tattattaag 180

aagcccgtag ctgtacattc ccgtgcccgt gttcgcaaaa acactgaagc ccgcaggaaa 240

ggaaggcact gcggttttgg taaaaggaag ggtactgcta atgcccgtac cccgcaaaag 300

gaattatgga ttcaacgcat gagagttttg cgtcgtctcc ttaaaaaata cagggaagct 360

aaaaaaattg acagacatct ataccactca ctctacatga aggccaaggg taacgtattc 420

aagaacaagc gtgtccttat ggaatacatc cacaagaaga aggcagagaa agcccgtgcc 480

aagatgttgg cagaccaggc caatgccaga aggatgaagg taaaacaggc tagagaaaga 540

cgtgaggaac gtatcgccac aaagaaacaa gaagttttgc agaactacat gagggaggat 600

gaagctgcgg ccactaagaa ataagttaat tgttttataa gatgactata tt 652

<210>700

<211>890

<212>DNA

<213>Diabrotica virgifera

<400>700

ggaaaaggaa gtgggggtga ataggccaaa caatgacaat ggttttagct attaaagatg 60

acgacaacaa gtttgatatg ttggtaagga actgtatggc tcacgatgga aaaagagctc 120

ccatacagtt agtcgaccaa agaggttgtg tcacaagacc aaaattgatg tccagattta 180

ctaagattaa aaactttggc gcaagcgctt cagtgttatc atatgcccac ttccaggcat 240

ttaaattccc tgactccatg gaagttcact tccagtgcac catccaaatc tgcagatatc 300

aatgtccaga tcagtgttca gattcttcag gacaacatct ccacggcttg cttgatttgg 360

tgcatggtcc acctgatact ggttatggtg ctcctccacc gctacctatc gaagcatatt 420

tacatcctgg gcgaccaagg gatgaaagga gatcaaagag agctctcgct gtcgacccgg 480

aaaaggaagt gggggtgaat aggattatta gggttgtatc tacaggagat ttgacattta 540

cacttgacga aaacagcaat agcacaactc cacccactat ggtctttccc gccaaggagg 600

aaattaacaa cggaggatta atatgtatga ctactccagg ttttgcagca accttagtag 660

ttttattagc tatattaata gtatcttgtc ttatgtcagc tttcctttgt gtcaaactaa 720

gaccatttgc tgaaaacaac tcgcaggtgt cataggatat gccaattcac agaaaacaaa 780

gtcaaggagc tgcttttact cgtaatatta gagcagactg aaaggggtta gatcgaaaag 840

aaaacggcta catgcttggt catataaatt aaaaatcttt cgacatatgg 890

<210>701

<211>888

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(775)

＜223〉n=a, c, g or t/u

<400>701

cccacgcgtc cggcggctga taaaagccta atgattggag ctgatgtagc agttgcttac 60

attgatggat tcagagggtt tgctacggac tacaatataa cagctttaac accatgtgta 120

aaagtattag gccaatataa aggagtatgc aaggatgaaa tggtaggagg acaggatagc 180

aaccaaatga atacggcagc gagggaaaat ggtattagca taataactta cagaagatcc 240

ttaatttcac ctgatcctgg agataaagag tttccagcag agggttccat atacgccgta 300

tggggggtag gaaaactaga cgaaatgaaa gaacctgctt ttcatgatct ctactcaaag 360

actgatataa aaatggagat gacaccgaaa gaacctcaga ataactgtgt ctcttttacg 420

agatcgaaac ctgaacccaa agaaccttgg aagaaaggtc aaatattcga taaaactata 480

aggatattta agtcttatat tggtccatca ggggccaaaa ggggatacca agccattacc 540

ggccactcat ccacagcttt agcatggtac ataaacggtt tcctcagccc tgagatatgg 600

ctacgacgag gtctttcata tgcatttaaa gtatatggag gaaacaatcc acacagtgct 660

gaatattatc atccattgat aataacagat gaaccccacg gaggttttga tagacttact 720

gacgatgctc agtctaaaat aagagtcttg gctggtgttg aatattctag aaggngaaga 780

cctagaccaa ctacatctgg tgtcctttgt ctatcagaac acaaagaagg aaaagacaga 840

agactcgatg atgacttcga aacgttcaaa aatttcacaa atcttgat 888

<210>702

<211>953

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(840)..(878)

＜223〉n=a, c, g or t/u

<400>702

aaatcgtgtc tgatataaga tacaaaactt tggaagaaga cgaaatctat actcttctca 60

ttattgaagt tgttccagaa gataccggta aatatgaatg tgtcgctatc aacaactccg 120

gagaagctag atgtgaagct gaatgtgtag ttacttcacc atctgctgcc cagaaacccg 180

ccaaacccac aactcccggt gcggaaaagg ctccagccct tctagaacct ctcaaagatc 240

agactattaa agaaggacaa gctgtcgtgt tcagatgtaa gatttcagct aaaccaactc 300

cagttattaa gtggcaaaag gctgataagg tcatcaagcc atctaaatac ttccaaatga 360

ccaaagaagg ggacttctgc acgttaaaga tctcagaagc tttccctgaa gatgaaggag 420

tctacaaatg cgtcgctgaa aatcctgcag gcgctgttag tactcaagct aagttgaagg 480

ttattgcacc agaaacgcaa gaagttgcac catcactaac cccaatgaaa gacgttactg 540

tacctgaagg tagccctgct caattcaaga ctactatttc tggaaaacct aagcccacca 600

tccaatggtt gagggaggga ttccttattc cagaatcgcc agatttccag atgatccaag 660

aagggaacaa cgttattcta ttgatttcct caacctacga agaagacact ggcttattca 720

cctgcagagc aacttcatca gccggtcaag ttgaacaatc tgcgaaacta accgtaaaaa 780

gtaagaaatg attgctataa aaatattctt ttctaattct caaattgtta atgtccatcn 840

catgtagtta ttgttangta tgacagcttn cagctggnta tttaacacag tataaataag 900

tgatcagtta gcagaactct tttgagatag tcatgatatt ggactaatgg gtt 953

<210>703

<211>905

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(849)

＜223〉n=a, c, g or t/u

<400>703

ccttacgcgt ccgccaggaa cagggaagga gaagcctcag tcacatttac tctgaaaatc 60

aagaaaatca agaagactca ggaaggcaag tttaagatag tcgccaggaa cagggaagga 120

gaagcctcag taacatttac tctgaaagta attacttcag agaaagaagc acctgaggtc 180

ttatcacctc tagaatctat taccgtaagg aaaggagaac cagttacact ttccactact 240

atatttggaa acccggaacc tactatcgaa tggttgaagg atggtaaacc tttaaccaga 300

ccaaaattca aaaaagacgg aaacacatac actttaacta ttcccaagac atctacagat 360

gacactgcta catatacagt tcgagctacc aattctatgg gtacaataga aacaagtgca 420

aacctaactg ttgaagaatt cccagaaaac caggaaccac cattgttcgt caaacgattt 480

gaagagcaaa ctgtaccaca aaagtctcct ctcaccctca aagccaaagt ggttggcaat 540

cctactcctg aagtatactg gcttagaaat aatgagcctt tggaaccatc cgatagggta 600

aagatcacct tcgatggaga aaacattgaa ctaactatta aagaaaccaa ttctgaactt 660

gactctggag actacaaatg tattgctgtg aatccagcag gcaaagcctc gcatggtgcc 720

aaggtatcta ttgaggtaga tactgtcaaa tttactaaag aactgaaaga agtttacgaa 780

acaatagaaa gaaagaccat cgaattagaa tgtgaaacat cccactcagt tagaacaaaa 840

tggtggtanc acacacggaa attagtggta tggaccatcg cgttgtagta caagatggac 900

gtacc 905

<210>704

<211>426

<212>DNA

<213>Diabrotica virgifera

<400>704

gtcctgggga ggctattcaa aaactcagag agactgaaga aatgttaata aaaaaacagg 60

attttttaga aaagaagata gaagaattta ccatggtagc aaagaaaaat gcgtcgaaaa 120

ataaaagagt tgcactccaa gccctcaaaa agaagaaacg attggaaaag acccaactac 180

aaatagatgg aacccttaca actattgaaa tgcagaggga agccctcgaa ggagctagca 240

caaatactgc tgtattagat tctatgaaaa atgctgcaga tgcccttaag aaagctcata 300

agaatttgaa tgtagatgat gttcacgata tcatggatga catagccgaa caacacgaca 360

tagccaacga aatcacaaac gctattagca atcctgtcgg attcaccgac gatctggatg 420

acgatg 426

<210>705

<211>473

<212>DNA

<213>Diabrotica virgifera

<400>705

gtctaggcac ggacaaaagg gaacgtgcgg tatacaatac cgtcaagagg atatgatttt 60

tagtgctgaa ggtattacgc cagacattat cattaatcct cacgctatcc cctcccgtat 120

gacgattggt cacttgatcg agtgcatcca aggtaaggta tcatccaata aaggagaaat 180

cggtgatgct acacctttca acgatgccgt caacgtacag aaaatctcca ctttgttaca 240

agaatatggt tatcaactca gaggcaacga agtaatgtac aacggccata ctggacgtaa 300

gataaatgct cagattttct taggacctac ctattatcaa agattgaagc acacggtgga 360

cgataagatt cattcaagag ctagaggacc gttgcagatt ctcgtcagac agcctatgga 420

gggtcgtgca agagacggcg gtctacgttt cggtgagatg gaacgagatt gtc 473

<210>706

<211>677

<212>DNA

<213>Diabrotica virgifera

<400>706

gtctaggcac ggacaaaagg gaacgtgcgg tatacaatac cgtcaagagg atatgatttt 60

tagtgctgaa ggtattactc cagacattat catcaatcct cacgctatcc cctcccgtat 120

gacgattggt cacttgatcg agtgcatcca aggtaaggta tcatccaata aaggagaaat 180

cggtgatgct acacctttca acgatgccgt caacgtacag aaaatctcca ctttgttaca 240

agaatatggt tatcaactca gaggcaacga agtaatgtac aacggccaca ctggacgtaa 300

gataaatgct cagattttct taggacctac ttactatcaa agattgaagc acatggtgga 360

cgataagatc cattcaagag ctagaggacc gttgcagatt ctcgtcagac agcctatgga 420

gggccgtgca agagacggtg gtctacgttt cggtgagatg gaacgagatt gtcaaatttc 480

acacggtgct gcacaattct taagagagag gctgtttgaa gtttccgatc cttatagaat 540

ccatatttgc aacttctgtg gtttgatagc tatcgccaat ttgcgtaaca acacctttga 600

atgtaagggt tgcaaaaata agacacagat atcgcaagtt agactgcctt atgctgcgaa 660

attactgttt caagagc 677

<210>707

<211>250

<212>DNA

<213>Diabrotica virgifera

<400>707

atggcctgta cgtcaagcaa ggccagtcag tgaaaaatta cctgccaacc gaaattgacg 60

ggcacactga atctattatg aaacgtaccg cattggtcgc gtttggcttc cttctacgaa 120

cgtgctggtc gcgttaaatg tttaggtaat attgtgcagc tggtaggtaa agcatctctg 180

gcagaaacgg acaaaatcac gatggtgaag ctaaaatcaa ggcagatttt gatcaattat 240

atgaagatat 250

<210>708

<211>250

<212>DNA

<213>Diabrotica virgifera

<400>708

attgtgcagc tggtaggtaa agcatctctg gcagaaacgg acaaaatcac gaaattgacg 60

ggcacactga atctattatg aaacgtaccg cattggtcgc atggcctgta cgtcaagcaa 120

ggccagtcag tgaaaaatta cctgccaacc gatggtgaag ctaaaatcaa ggcagatttt 180

gatcaattat atgaagatat gtttggcttc cttctacgaa cgtgctggtc gcgttaaatg 240

tttaggtaat 250

<210>709

<211>250

<212>DNA

<213>Diabrotica virgifera

<400>709

gaatacttcc gtgatatggg ttacaacgta tctatgatgg ctgactcgac gaatacttcc 60

gtgatatggg ttacaacgta tctatgatgg ctgactcgac gaatacttcc gtgatatggg 120

ttacaacgta tctatgatgg ctgactcgac gaatacttcc gtgatatggg ttacaacgta 180

tctatgatgg ctgactcgac gaatacttcc gtgatatggg ttacaacgta tctatgatgg 240

ctgactcgac 250

<210>710

<211>273

<212>DNA

<213>Diabrotica virgifera

<400>710

gaagaaagta cgttaatatg gttccagggc tccaacaaga cgagctattc ttactgggtc 60

gagaacattt taaaattttt ggataagtac tacgttccca gcaaaatagc taaaggaaat 120

ggccaaatta aaacatgctc tcatcacgac tatcctacta gtggagaagt atgcgaagtc 180

gatgtcagag attgggaaga atgcaacagg gatcaattct ttaattatca caagaattct 240

ccatgtattt ttattaaatt gaacaaaata tat 273

<210>711

<211>278

<212>DNA

<213>Diabrotica virgifera

<400>711

acatttttcc cgccattcca attactatta agttttttcg ttttaatact tttttaaata 60

tacaggtgct taatatcgtt tatattttca gtattacttg gttttcttca tgtaaattgt 120

tttaaatttt tcttttaccc ttttaatctt gtatattaca ttacccaatt aaagttaatt 180

gtacagatta agataaacga gtatcttata acatctatta gattgttaga atcaataaat 240

gtagtgtaat tgttctgttt tgaacaaata aatgcatc 278

<210>712

<211>287

<212>DNA

<213>Diabrotica virgifera

<400>712

aacggttatt tggaaggcct gtgtcgtggc tttaaatgtg ggatcctgaa acaatccgat 60

tatttgaatt tggtccagtg tgaaactctt gaagatttaa aactgcactt gcaaggcact 120

gactatggaa cttttttggc caatgaacct tcacctttgt cagtatccgt catcgattca 180

agacttcgag aaaaactcgt gattgagttc cagcacatgc gtaaccaagc agtagagcct 240

ctctcgacat ttatggactt cattacctac agttacatga tcgacaa 287

<210>713

<211>250

<212>DNA

<213>Diabrotica virgifera

<400>713

atggcctgta cgtcaagcaa ggccagtcag tgaaaaatta cctgccaacc gaaattgacg 60

ggcacactga atctattatg aaacgtaccg cattggtcgc gtttggcttc cttctacgaa 120

cgtgctggtc gcgttaaatg tttaggtaat attgtgcagc tggtaggtaa agcatctctg 180

gcagaaacgg acaaaatcac gatggtgaag ctaaaatcaa ggcagatttt gatcaattat 240

atgaagatat 250

<210>714

<211>250

<212>DNA

<213>Diabrotica virgifera

<400>714

attgtgcagc tggtaggtaa agcatctctg gcagaaacgg acaaaatcac gaaattgacg 60

ggcacactga atctattatg aaacgtaccg cattggtcgc atggcctgta cgtcaagcaa 120

ggccagtcag tgaaaaatta cctgccaacc gatggtgaag ctaaaatcaa ggcagatttt 180

gatcaattat atgaagatat gtttggcttc cttctacgaa cgtgctggtc gcgttaaatg 240

tttaggtaat 250

<210>715

<211>250

<212>DNA

<213>Diabrotica virgifera

<400>715

gaatacttcc gtgatatggg ttacaacgta tctatgatgg ctgactcgac gaatacttcc 60

gtgatatggg ttacaacgta tctatgatgg ctgactcgac gaatacttcc gtgatatggg 120

ttacaacgta tctatgatgg ctgactcgac gaatacttcc gtgatatggg ttacaacgta 180

tctatgatgg ctgactcgac gaatacttcc gtgatatggg ttacaacgta tctatgatgg 240

ctgactcgac 250

<210>716

<211>372

<212>DNA

<213>Diabrotica virgifera

<400>716

cccgggttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 60

gccctcttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 120

gccctcttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 180

gccctcttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 240

gccctcttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 300

gccctcttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 360

gccctctcta ga 372

<210>717

<211>372

<212>DNA

<213>Diabrotica virgifera

<400>717

cccgggcaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 60

gctagccaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 120

gctagccaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 180

gctagccaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 240

gctagccaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 300

gctagccaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 360

gctagctcta ga 372

<210>718

<211>490

<212>DNA

<213>Diabrotica virgifera

<400>718

catcacgact atcctactag tggagaagta tgcgaagtcg atgtcagaga ttgggaagaa 60

tgcaacaggg ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt 120

tgctactaaa ccaatcaaac gatgaggaac agacagaaca aaccgcaact gaaacggcaa 180

ctacagaggt acagaaaaag ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca 240

cacggtgctg cacaattctt aagagagagg ctgtttgaag tctcttcagc aatagacaat 300

ttgactccct ttcctcttgg gagggaaacg aaggccttag ctcccttacc tcatcgtccg 360

tttggattca caaaaacaca tcgacttctc tctgaaatca cctttgggag gcggtcgtcc 420

gatccttttc gatttgttgc tgccattggg gatccatctg gacttctgga ggaagcaagg 480

caggt gcagc 490

<210>719

<211>560

<212>DNA

<213>Diabrotica virgifera

<400>719

ggcgcaagcg cttcagtgtt atcatatgcc cacttccagg catttaaatt ccctgactcc 60

atggaagttc tccatcaggg gccaaaaggg gataccaagc cattaccggc cactcatcca 120

cagctttagc atggtacata tagtgatccc aaccgtgcac ctgactgtga cccaactcaa 180

tgtgtaattc cggactgttt ctgctcagcg cccgccacca gttagcgccg aagtacaaaa 240

gggattggac cttatttttg ctggtatcag agctcaacag gcaccacgtg ctcaacgata 300

aaagactggt caggaaggca cattcctttt tgccttactt attcaaggca gggtgcatac 360

agagtagcca cgtaacaaat agcacatagc gaagtgttgt accagcataa tgtataagct 420

cctgtactta ccaaaggctc gttggcagat ctagaccagt tggacgaatt ggttattgag 480

tactgtggtg cagcgccccc tttatggcag atgaatgtct gatggccata ccagacatcg 540

aaggtattga ttacaccacc 560

<210>720

<211>372

<212>DNA

<213>Diabrotica virgifera

<400>720

cccggggcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 60

ggagaagcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 120

ggagaagcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 180

ggagaagcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 240

ggagaagcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 300

ggagaagcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 360

ggagaatcta ga 372

<210>721

<211>372

<212>DNA

<213>Diabrotica virgifera

<400>721

cccgggctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 60

acgatgctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 120

acgatgctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 180

acgatgctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 240

acgatgctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 300

acgatgctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 360

acgatgtcta ga 372

<210>722

<211>1000

<212>DNA

<213>Diabrotica virgifera

<400>722

caagcccgcc aaaatgcgcg aacatctgga actcttctgg tctcgagtga atatccccaa 60

agttcttagg aatgcaatgc ttcctagacg tcgtcgagct ggctctgaaa ctgagcccca 120

agttatagta ggtaacgata attattggac gtggcattag gttataggga aagtgtcaat 180

ctgtctcaaa ttcgcacatt tgtggaaatg gactcgcacg cgcggcccaa cgacggcttc 240

aacaaacaca ggcgcaagtc gacgaagtcg tggatatcat gaaaacgaat gtagaaaaag 300

gtatggcagg aaagaaatgc tgaatccgcc attgaagctc ttaaagaata tgaaccagaa 360

atgggcaaag ttgtccgaca agacaaatcc ggtattcaga aaatccgggc atagcaaaca 420

tgccaaacac acacacgcag aaaggaaata tcagtcagac tcagaatcag atagtgatct 480

ggaatggatt ggacacagag gtgaatcaga ccagaggacg ttcgggggta tattaaaaat 540

aacgttgtgt gcgcacttgg aaccgaaaca agcagaccat catccgcagc tcagtgcgta 600

gccgaatgtg gccgtgcaca ctgtgcattt gtaggacagg tgtgcgggtt tcaacgtcat 660

gttatgatcc cttgcatata ttgatggaaa aacacgccaa agaagagagc agagtattaa 720

aaggagtatt gccaggtaca tagaccagca aggaactgta atcaaaatgg gcgatgaaaa 780

gaaggccagc gatagcgaac acattaatct caaagtactt ggtcaggata atgcagtggt 840

gcagaaggtc agacctctcc tcaacgtcac ccgtatcgag gatgagattg ccaaacttga 900

agagaaagcc caaaaagccc aacgaacacg gcgtagacga aacatcttgt gtagttattg 960

tagaaggaca aaccagcaca ccagaacaac aagaactagc 1000

<210>723

<211>478

<212>DNA

<213>Diabrotica virgifera

<400>723

cctcaagctg tcattggcat ggatattctg tgccaagcta aatccggtat gggaaaaacg 60

gctgtttttg caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc 120

aaatttcttc ctccagtgat catacccata cgatttacct gaggaaatgc cggataatct 180

gaagcaacat ataaggggta tcaacaatac tagagagttg agtcaaacat aattatggca 240

cattgtcctt ggatgtagta tccatcacgc agaccgccga acaacacgac atagccaacg 300

aaatcacaaa cgctattagc aatcctgtcg gattcaccgt aatgcactcc tacgtcgttt 360

ggttcgtatt ggagtactag acgagaacag aatgaagctc gattacgtat tgggtctccg 420

aagtaatgta caacggccac actggacgta agataaatgc tcagattttc ttaggacc 478

<210>724

<211>584

<212>DNA

<213>Diabrotica virgifera

<400>724

cacttgacga aaacagcaat agcacaactc cacccactat ggtctttccc gccaaggagg 60

aaattaacat aaagaacaag gagaagccgg taattacaaa gctgcatatg aaaccggaaa 120

caacattatc gctgaagaag gaggttactt gaaggcaagg atgaaatggt aggaggacag 180

gatagcaacc aaatgaatac ggcagcgagg gaaaatggta ttgttgccac atggtagact 240

cctgttctaa tgttcaaact ggagatcaat ttgcaagact actcagacac aatttcaaca 300

gacatttgaa ttcaaaccga gctccgttgg gtccagccac ataggactaa gtgtggaggg 360

ttcattcatc aaaattcgct ttatgtgcac acatagcaca taagcataca tggcaaatat 420

gcctatccat ccagttgtca gctaacagaa gtgcccttat ggagggtgtt ttgaagaaca 480

cgctggcgaa tgtgagttgc agatgggctc aagaatacac cgctattgac caggatctcg 540

cttcagaata attccataga aaagctacca gcccatgcat ttaa 584

<210>725

<211>869

<212>DNA

<213>Diabrotica virgifera

<400>725

ccgaagtgag atcggagcca aaaagcattg gttgcttgac ggtccaatgg aaaacgaacg 60

agaagtggat ggcttcagca atgctcggcc aggctgattg ctctttattt tgacactgga 120

atgtacagcg aagcgctaca actggaatcg accttactga ctactcgtct acgatatcgc 180

caaacatctg acatacgaaa acgttgaacg ttggttgcgc gaattgcgcc attcttttct 240

caggtaccaa tgttgctgct ggtaaagcac gtggtattgt tattggaact ggtcttaaca 300

ccgccattgg taaaatcaga actgaaatgt ccaacgaaat tgccaacact aactcaagac 360

agaacatccg taagttgata aaggatggtc ttattattaa gaagcccgta gctgtacatt 420

cccgtgccga tacctacaca gaaagctata ttagtaccat tggcgtagat tttaaaatca 480

ggacaatcga tttagatggg ttacaaaagg ataccaatac aagatgagag cagcatatgc 540

tcactttccc attaactgtg tcaccactga aggaaacaca gttatccagt ttgcaagctc 600

tctggcagat ttcagtcaaa tcggcaccag aaaatccatg agttacttta gcaatgtata 660

caagatctac gtctttagct acaggcgatt tccgatgaaa tagtggcatt aggttatagg 720

gaaagtgtca atctgtctca aattcgcaca tttgtggaaa tggactcgca cgaagaaaaa 780

gtttatcagg ccgtgagaga attaaaagtt caaggaggta tcggttcgtg tgtatcactt 840

aacgtgaaga gccccttggt ttccgacac 869

<210>726

<211>737

<212>DNA

<213>Diabrotica virgifera

<400>726

gtggcgtttt tctatttaca gtatgtgaat gtgtcaaagt aactgtgttt ttcaaaaatt 60

aacaaaagaa attcaaaatg tacaagtgga caaaaaatat taaacaaatg tatatagtgt 120

atctatttct aatcgaatca acaaaatatg atcttactga acagaatatg atgcaagcta 180

atattaaatg caactcgtat gttttattat atcctaaacc aaagacttat tcatcttgaa 240

ggtgactgtg tgccgtcatc tcttttattg agacaattag gacgtttttc gttctcagcg 300

gcttctcgga aatccctggc ttacagaaac ggatggtgac tatagtcacg gaactgtcaa 360

aattaatttt gtatcttata tgaagcctca aacgttttgg aaatatttct tctctttatc 420

aggaactgga aagaagtata tgccctcagt ttgttatctt tctctagatg atgggaggaa 480

aaatttttaa aatacctgtt tgtcagagtt ttattactat taagaagaat tcttcatctt 540

aaaggtaacc gggtgtcgtc atcactttta ttgacacaat taggacgttt tttgatctca 600

atggcttttg gtgtggacaa tagttataga actttcaaaa ccaattttgt gtcctgtcta 660

gatcatcaaa cggttttgag aagatttctt cccttcatat ggagctggaa agaagtattt 720

gacacctgtt agttatc 737

<210>727

<211>867

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(771)

＜223〉n=a, c, g or t/u

<400>727

gcagtttgag aggaaagttt caacaccccg tatcttggaa acgaagcatt tgcggacata 60

cgtttataga gcaaactgtc attatttaac accctgtata ggcccacaca aaagattatt 120

acctccttca tgagactttt ttttttattg agttattcat gtcgagttgg acgtttctga 180

tatgaaaaat tttcaggagg gagacggttc gtaaatatgg gggtttataa actttggtgc 240

atctatggtg gttcctgttt atctgaacca tctcagatag ttaaggggtg gttaccccct 300

actatagggg ttgatgaagt aacactactc acagtatatt catttattta taagttgaag 360

taactacggt aaagagttgg tggctaaata tttatccgct gtgatgttta cccgctggga 420

tctcaaatac ccatgattaa tctttacgca atgaatttag aacgtcgaat gtggaatccc 480

ctgtttatgt tttggtatat ataaaaagtg aaagtgtttg tattataaat gctgaatcaa 540

cttgattata aataaagaat aatatttgct actacagctg accttgcatt atcgataact 600

gatacacttg tatagtaata taggtcaatc atgtttatga taaacaacgc gtattcgttt 660

accaaggaca tttaaatatt aaaagaatta gtcttgccct taataagatg gttactaaga 720

gatgctgtga atcccaatac acatccgaca actggagtaa gtatccttga naatttgtcg 780

gaaaatatta ccagtgaatt gtatttttat caaaatatac tgcaaaaaaa cccatcattt 840

atgactctat agtggacgca catatcc 867

<210>728

<211>802

<212>DNA

<213>Diabrotica virgifera

<400>728

cgtccgtgat aacaaaatca atcaaagagt tgtttattaa atagtattga tagtgtattt 60

gaaaaataat aatttattta aggcgtacaa ttaataaaaa gttatttatt gtttatatat 120

tatttatgga aaatccaagt atttcaagtc attaaaattc aaataaaagt attattttat 180

aagagtttgt tccgataacc gtaaataggt atgagtttag ttataaagta aagtgcccat 240

ggtgtcataa aatgtagtaa atgctaatgc ttcaagtacg tactacattt ttgaagattt 300

cactgcactt aaaaagcatt tgcctttctc cgcagtattt gctactattt accagcctac 360

atagaagaat gtactacgct tttgaagtat atgcttgttt agtagtattt tgcttcagtt 420

taagtgaagt tggtgggtgg tcgtcttcgg cgtaggtatt tttttgttac aatatggcag 480

catttatgaa tgtgtgtcat agaaaaattt acaaactaag aagaagattc ttaatttttg 540

gggtcattct tttatcaaat aaaatttaat aattgttaag aagattaaca aataacaata 600

attgttcatt tttatcctcg cgtaactctt tgtcgcttcg gatctgagtc actcattttt 660

actcatgtac aattctaata atttggtacg taaaaatgtg aatttacaaa tcacaagcac 720

atggaaaact aattccatca cgattggtgt aatgcagaca cggctggcac tacgctttgt 780

agtagatact tctggtcagt ag 802

<210>729

<211>862

<212>DNA

<213>Diabrotica virgifera

<400>729

ggaccatttt gagtgggagg atagctcaag tgttgttgtt tgagttattt tcgggcaatt 60

tctgcaggga agtttgggtc acctctcaac gtccacctca aaacagatgc gccctggact 120

aaaacaattt taagtactct cattgttaaa aagttgattc accacatttt cataacgatt 180

tttagggcgg ctgtacacgg actgctgagg tcgtttatca ctgagcgaca tacacggact 240

gctcgagcga tcgttgtaga cttctggagc ggtccattgt tgactacctg agttgtccct 300

tgtcgcattg caaccgctct cgatctgtcg atagcacgat ggaatttcat tatgctattg 360

acagcttgaa gcggtcttga ctgctcgagc tgtcgtgtgt atggcaacga atatacaatt 420

ataattcacc gcgcaaacgc tgcttcaaaa agtcgacctc aactgcttga agatgtccgt 480

gtacggccga tcttggggta tcttttttac tcttatcggg aatatttaag aaccgaaatc 540

cctaatgttt tccctatttg ttccagaatg atatacgtgt taatttccaa ctgagacttg 600

acggccagca cgaccgtacg acaagacatt ttcgaacagt aaaatcatta gttttagtaa 660

caaatacaaa gacagatggg aattcggttc tcgatattat gcagagtatt tacctgatga 720

tgaacactta ttggaggata gaaacgcata atttgcagaa ctgacttacc catgtaatgg 780

acaggtatga tggaaacgta tgtactttaa tgttttgtta gtatttttac gagagaagct 840

aagtaagttt gtgaagaatt ag 862

<210>730

<211>603

<212>DNA

<213>Diabrotica virgifera

<400>730

gctgctcctg tggcataacg gccccgtacc tggagaattt tacaattttc ccggcaaaca 60

gaccagctat tccaacccta taacacatac acagtctccc gttacaacat caacttctgt 120

attagctatt acttacgata aaggagtatt gataaccgga gatctggttg cttcatacgg 180

atctttagct aggtacagaa attgcccgag ggttattaaa gtcaatcaga atatcatctt 240

gggagccgga ggagactatg ctgatttcca atacgtcaag agtttcattg agcaaaaaat 300

aattgatgaa gaatgcattg atgatggact aaaaatgaaa ccaaaatcat tgtactgctg 360

gcttactaga gtaatgtacc aacgcaggag taaattagac cctttttgga ataatctggt 420

tataggagga atccaggatg gagtcccctt tcttggaacc gtagataaat tgggaactgc 480

atatacagac aaagttatat gtacaggtta tggagcccac atagccttgc ctcttctgag 540

agactcttta gataaaaatg gagctcttaa cttggccgat gccaagactc tgattcacaa 600

atg 603

<210>731

<211>545

<212>DNA

<213>Diabrotica virgifera

<400>731

ggtactttgg gttgtacttg ggataaaaga cctttttgtg tttccaaata cattgtcata 60

taaattaaaa aagaaatggc tgccatcaga tctatgtctc gccttcttat taatagatca 120

cttcaaacca ctgcaaagag gacctatgcc gaccaaatga acttcacatt tgcagctgga 180

aatcaggttt actatactgc aacttcagtt aaacaagtag atgtgccatc tttctctggt 240

agctttggta tcctccccgc ccatgtaccc acactggctg tgctaaaacc tggagtagtc 300

actgtttacg aagatggtgg taatgttaag aaaatctttg tttccagtgg tactgtcacc 360

atcaatgatg attcatcagt tcagattctt gctgaagaag cccacccagt agaaaacatt 420

gatgtcaatg cagcaagaga catccttacc aaagcccaga gccagttatc atctgcaagt 480

acagatgagg cgaaagctga agcctccatt gccgttgaag tgggagaagc gtatcaaagc 540

ggctg 545

<210>732

<211>496

<212>DNA

<213>Diabrotica virgifera

<400>732

gactaaactc cacagaactc ccagacccag tcggcctgac aaggctcgca gaatgggtta 60

cagggccaag caaggttttg ttatttaccg tattagagta cgacgtggag gacgcaagcg 120

tccagtacca aaaggtgcca cctacggtaa acccaaaagc catggagtta atgaattgaa 180

acctgtccgt aacttacaag ctattgctga ggaacgtgta ggaagaaggt gtggtggatt 240

aagagttcta aactcctact gggtagccca agattcaaca tacaaatatt ttgaaatcat 300

tttggtcgac ccctcacata acgctattcg caaggacccc aaaattaact ggattgtcaa 360

ctcagtacac aaacacagag aactccgtgg caaaacttcc tctggcaaat catctcgtgg 420

tttgggcaag ggtcacagat actctcaaac caagggagga tccaggcgtg ccgcttggct 480

cagaaggaat cactgc 496

<210>733

<211>512

<212>DNA

<213>Diabrotica virgifera

<400>733

gctgattcgt tattctctgt gacaaagtac caacagttgt gtctttaata tctctcaata 60

agtgttcaac accaacttct tcagcttcct cagctccgat ttcactagga acatgctcaa 120

atgttttaga tgtaggtgaa ccgtcatcgt gtacttcttc tacagcttga taagcttcag 180

ttggtaaacc taaatctttt ggtttagcgt ctatgataac tagaacggaa tttggacaat 240

accttctgat taactcatta atggcaatat cattttgatg aagtttgggg ccggtgtggt 300

accaacctac tactcgttcc ctggcattaa cttttttgaa cattccatac atgttttcta 360

agtaatcatg gtccaagaac cacacagact tatccttgtc atcttcgtca aaaggaactg 420

caaaactgtt agaaacatct aagactcctt tagcacgcca acagccaaga agaacaccaa 480

caacacgctt ttgattgccg attttgccca tg 512

<210>734

<211>669

<212>DNA

<213>Diabrotica virgifera

<400>734

gttgacgttg cgtccgaaca gagcattacg gatctgggga atcaattgtt gtgagatgag 60

ctcaagacgg gcttccagag tattgttgat tttgatcttg tttctcaagg ccaacagttc 120

gattcctccg gtggtttctt gagaaaggtg gctctcgtcg tcgattttta gatttacgtc 180

tttaccggtt atgtccttgt acttttggga gacgttaggc atgatagatt ttaccaattc 240

tctgtcctga gggcgtactc taatggtgat gtccttttcg aagagctgat agagcccttg 300

gaggatgaga ctttccagga tttgtgtata tttgcctgaa tctctggtta cctcaccaag 360

acgtttgcga gcatcttcca aaacggcacg tacatggtct tcccttactt tcaatacctt 420

caatcttgcc tggttcaaca tgtttgatga ttggattttt ttctggagtt ctacttgctt 480

ctcttttttc tcgtagtact ccataatctt gagcctctgt tgttggacca gacggccctt 540

ttcgatgttg aattcttctt cagcctttgc atcaatttcc tcggcctttt cattggcttc 600

ttgctcaatg aaagccatca tgtgcttgat ctgcttttgt acatctgcgt cgctgagagc 660

attttgaga 669

<210>735

<211>802

<212>DNA

<213>Diabrotica virgifera

<400>735

gagccaagag caagtctgaa gatggttgac ggaaaaccaa acgagagtac cactagcaaa 60

gcagccttga aaaaggaagc aaaaaaggca gagaaggcag caaagaaagc agaacacaaa 120

caagctgctg gtgaaactca acagaacgtt gtagaagaag ttgacatatc agttggaaaa 180

tatggaaatc ttccccttat aaaatcagaa actaagcaca acgagaggac tttcacctca 240

gtaagtgacc taaataaatc cgtgtctgga aaaactgtat ggataagagc aaggttacat 300

acttcaagat caagaggaaa acaatgcttt attgttctga gacaaaaaga atttactgtg 360

caagccattt taaatgtctc agacgtcgtt tcaaaatcta tggttaaatt ttcaagtggg 420

gtacctaaag aaagtatcct cgatgtcgaa gccaaagtat cagtagtgcc caataacata 480

gcaagttgta gtcaacagga cgtggaacta gttgtttcac agttatgggt agtgtccttg 540

tctgctccac agcttccttt gcaaatagag gatgcatcca gacctgaaaa accagaggaa 600

gaagaaggtt taaatatccg ggtaaatcaa gacacaaggc tggacaatag aattttagac 660

ttgagaacac caactaacca agcaatttac cgattggaag gaggcgtctg tcggctgttt 720

agggaagcgc ttactaagaa agggttcgta gaaatacata cgccaaagat catatctgct 780

gcttcggaag gaggtgctaa cg 802

<210>736

<211>579

<212>DNA

<213>Diabrotica virgifera

<400>736

gcagtcccag aatcagtctt aaaacaccgt aaaagacgtg aagcatcccg tgctaaacaa 60

ctccagtctg tcatcaaaaa gaaggcagaa cagatcaaaa agaggaaaga tattttcaaa 120

agagctgaac agtatgttaa ggaataccgt ttaaaagaaa gagacgaagt taggctagtt 180

agacaagcca aaaccagagg aaatttctat gttcctgctg aggctaaatt ggcttttgtt 240

gttcgtatta agggtatcaa taaagtagca cctaaagtac gcaaagtact ccaactattc 300

cggttacttc aaatcaacaa tggagttttc gttaagttga acaaggctac tattaacatg 360

cttaggatat gcgaacctta cattacatgg ggatatccta acttaaagtc agtaagagaa 420

ttaatctaca aaagaggatt tgccaaagtt aatggacagc gtgtagcaat taccagcaat 480

caaatcgttg aagacaggtt gggaaaatct ggaatcattt gtgttgaaga tttaatccac 540

gaaattttca ctgttggacc taagttcaaa tatgcctcc 579

<210>737

<211>640

<212>DNA

<213>Diabrotica virgifera

<400>737

gttcagaacc aattacacga ataaagcagg catctgttct attggctact gctctagcac 60

aaagagtttt accagttcca ggggggccaa aaaggagaac gccttttggt ggttcaatac 120

caagttttac aaatttttca ggatggagta aaggtgtttc aacaacttct ctcaactttt 180

caatttgttc cttgcagcca ccaacatcac tgtatgtaac atcaggcttc tcctcaactt 240

gcatcatagt cacagtggga tcaatttttg gaggtagagg aatatgaatt tgatacttat 300

ttcggtcgac tccaactctc atgccttctt ctatgtctgt aggggccaca gagtctgcca 360

aatctactac gaatttggca aactgtttaa cgtttataat gtatttggga tcatctgtat 420

cagcgttgat aattttcgta caacgggcca cttgtaaggg ttgctcattt tgtaaggttt 480

gtttgtcagc tgccaaatcc cataaagctg gtggagctaa accagtatca gattctttta 540

taccagttaa ttcatttact cttttgatta tcgtttggat atcttcttca catgttttaa 600

ttgtctttgt gtattgccct tgtccatagg ttttaagaag 640

<210>738

<211>669

<212>DNA

<213>Diabrotica virgifera

<400>738

ccaccttcaa atgcgtcttg gtcggagatg gaggtaccgg caaaactacc tttgtaaaaa 60

gacacatgac tggtgaattc gaaaagaaat atgtagcaac cttaggagta gaagttcacc 120

ctttagtttt ccacacaaat agaggacaaa ttcgttttaa cgtatgggac acagcaggcc 180

aagaaaagtt tggtggtctg cgtgatggat actacatcca aggacaatgt gccataatta 240

tgttcgacgt tacatctaga gtcacttaca aaaatgttcc aaactggcac agagatcttg 300

ttagagtttg tgaaaatatc ccaattgtat tgtgtggtaa caaagttgac atcaaagata 360

ggaaagttaa ggcaaagagc attgtcttcc ataggaagaa gaatttacaa tactatgaca 420

tctctgcaaa atccaactac aactttgaaa aacccttctt gtggttggct aggaaactca 480

ttggagatcc caacttagaa ttcgtagctg cacctgcctt gcttcctcca gaagtccaga 540

tggatcccca atggcagcaa caaatcgaaa aggatcttaa agaagcctca gaaactgcac 600

tgcccgatga tgacgaagat ttataaactc attaaccaat tactaaaact ctgtcatcgt 660

tccaaagcg 669

<210>739

<211>302

<212>DNA

<213>Diabrotica virgifera

<400>739

catcctctgt gtttccgttt tgaacttaac ttgtatttag tttaaaaaat aatcatgtct 60

agaggaagca gtgcaggttt tgaccgacac ataacaattt tctcgcccga aggccgactc 120

tatcaagtag agtatgcttt taaagccatt aaccaagccg gccccacttt ggtagcagtc 180

cgaggagtag atgctgcggc gtgtgtgacc cagagaaaga tcccggataa gctgattgat 240

cccaacacaa ttacacatct gttccagtta acagaacaca ctggatgtgt gatgactggc 300

at 302

<210>740

<211>838

<212>DNA

<213>Diabrotica virgifera

<400>740

gaacttgttt aactacttga agtacattgg ctggcggtgt ttgtggtttt agaccaattc 60

caggttggtg aggagatggt attcccattt gaggatttgg caaacctgta ctcattgaat 120

tagcaggact aacagcacct ggtactactg attgcacacc agcaccaccc atagtgacat 180

tagcaccacc tggtatagga ttcgctgctg gtgctgttct agtcataaca gccattcttc 240

tcctaagtag ttgcgcctgt tgcaaccttt gttgcaattg ttgttgtttt agtttgtgct 300

ttatgtttga acagaatggt acaggacact tgttttctgg gcagtgtttg gcatgatagc 360

aacatagagc gatgagttgt ttgcaaatag ggcaaccacc atttgttttc ctcttgcaca 420

atttggtgtg atttgtgact cgtttcatct tctgacagct tggtaaccga caattggcat 480

ctctgcattg acaagcatgt accaaagaat ggatacaacg ctgtatagat aattttctag 540

cttctcctgg attggctttg ctatcactag gtgaacttcc atcatcaata tccaatccaa 600

gtttctccat tttgtgagga tgaccttctt tctcataaca ttggatgcac aagtcaaagt 660

cttcacacac agtacaatga taccttgttt ccacatgggc tttacaacta ttacaagtat 720

atacaaattt atcttgacct tgattatgca attcatataa catagacaaa gcactatatt 780

tggcccttct taaagaagag aattcataat gtttttcacg ggcgagtgtt aggaatgc 838

<210>741

<211>1301

<212>DNA

<213>Diabrotica virgifera

<400>741

ttcaagttgt tcttgtcctt cttcgtaggc gcccttaagt ctgaacaatt cggtggagta 60

gtttctgcat tctttctgac tggcatcgag ttcagcagcc aagtcatcaa ccttgagttt 120

ccattctcca atgattttgt cgaaggcctt ttgtttcttc tcagcagcat tggcaatggc 180

gttagcacgg tcgacttcga gttgcaagtc ttcgacttca gtggccaaac gttgtttggt 240

cttttcaaga gctacaacct tctggttgag agattcgatg gtttcttcag cctcagcaag 300

acgagcttgg agtttcctct tagcttcttc gagttcttca gagcgagcaa ctccttcaga 360

ttcgtatttc tggcgccaga gttgagattc agcgttggct ttgctgagtt gtctttggat 420

atcagccttg gcttcggctt cttcttcaac ttgttcacgg atgttgtcca agtcgtgttc 480

caagtttctg aatttgccga ggagggtagc gcgttctctg ctctcttcat cggccaatct 540

cttggtatct tccaattgag tggtaaggga taccttgatc ttgctgagtt gagatacttg 600

agattcggct tcctcgagtt gtctgaggag gtcggagttt tcaatagaca acttcttctt 660

ggcggcatcg aagtcgttaa gagtgcggtt ggtttcgtca agtttgcctt ggacgtcgtt 720

gagttgttgt cccaattgtt tggatacctt ttctatagcg gccttttcgt tggctaagtg 780

gtcgactgaa gcacggaggt cattgagttc accgaagtat tgagactttt ctttctcagc 840

cttggctttg agtttgttga gttggtcaat ttgttcaccc atttctgaaa cagcgtcgtt 900

gtgcttcttg cggaggttag caagagttcc ttcatgttgg atgttggatt cttcgaggtc 960

acgacggagt ttggcgagtt cggcttcacg tttcttgttg agttcgattt gggctgaggt 1020

agcaccaccg gcttcttcaa gacgttcgcc gagttcttcc aattcgcggg ccaaatcagc 1080

gcgttgtttc tcagctttag cacgggcttg acgttcagct tcaacttcct cttcgagttc 1140

ctcaatgcga gcctggagtt ccttaacttg tttttgttgt ttaccaacaa cggattgttc 1200

gtcttcgagt ttggcagtga gtgatgagat ttccttgtct ttgcgttgga tggtttgttc 1260

gagttccttc ttgttgcgtt ccaagtcagc tacagcctct t 1301

<210>742

<211>824

<212>DNA

<213>Diabrotica virgifera

<400>742

cacctccaag gaaagtgtct ccattggttg attttacttc aaaaactccc ttttggattt 60

ccaagataga tatatcgaag gtaccacctc ccaaatcata aacagcaata actttatcct 120

ctgttttgtc catgccataa gctaaagctg cagctgtggg ttcatttatg actctcaaaa 180

cattaagccc agaaatttgt ccagcatctt tggtagcctg acgttgtgag tcattgaaat 240

aggcaggtac tgtgattaca gcattcttta ctttggtgtt taagtaggct tctgcagttt 300

ccttcatctt aaccaaaaca aatgctccta tctgactagg ggaatacatt tttccatcgc 360

ttccctgaac ccaagcatca ccatttgatg ctttaactat tttatatgat acagttttca 420

tatctttttt aacttcctca tcttcaaatc ttctaccaat caaacgttta gtggcataaa 480

atgtatttgc agaatttgtt acagcctgtc tcttagcggg cataccaact agacgttcat 540

tatctttact aaaggcagct actgaaggtg ttgttctgga accttctgca ttttcgataa 600

ctttggcttg tttaccttcc ataacagcaa cacatgagtt tgtagtaccc aaatcaatac 660

caataacagc ccccttgatt ccttctgatt tgtgtcgtaa ttgaatgtca tattttgggt 720

ttagagcgta cagggaagat ctattatttg acaaatggtt aaaatttctg tttccagctt 780

tttcgaaagt gattttcgag caatcgaggg ctttcctgct aagg 824

<210>743

<211>704

<212>DNA

<213>Diabrotica virgifera

<400>743

tgtagacctc tttgccaagg tatcggctgg agatttaaat cctataaatg ctactattgg 60

tggtattgta gcccaagaag taatgaaggc atgttctgga aaattccatc ccattttcca 120

atggttctac tatgatgcca tagaatgcct accacaagat aaatcagaaa tcactgaaga 180

actagctgca cccactggta gcagatatga tggccaagtt gctgtttttg gaaaagaatt 240

ccaaaagaag ttagcatcac tgaaatattt tattgttggt gctggagcta ttggttgtga 300

attgttgaag aactttgcta tgatgggtgt aggaacacaa ggagaagtta cagtaactga 360

tatggattta attgaaaaat ctaatttgaa caggcagttc cttttcagac cacataacgt 420

ccaacaacca aaatctgcca cagctgcaaa ggtcattaaa aagatgaatc ctgatatcaa 480

tgttgttgca catgaaaatc gtgttggacc tgaaactgaa agtgtctaca atgacgattt 540

ctttgaaaag cttgatggag ttgctaatgc actggataac gtagatgcta gaatttatat 600

ggatagaaga tgtgtctact accgcaagcc tcttctagag tctggaaccc taggaactaa 660

gggaaatact caagtggtgg caccttactg atacaacagg gggg 704

<210>744

<211>971

<212>DNA

<213>Diabrotica virgifera

<400>744

gacgtgcccc ttaagtcaaa ataccaaacg agaatccggt cgaaaagttc agctggaaaa 60

catcaatgcc ggaaagcaaa tcgcggatgt gataaggaca tgccttggtc ctcaagctat 120

gttaaaaatg cttatggatc caatgggagg tattgtgatg actaatgatg gcaatgctat 180

tttacgtgaa attacagtac agcatccagc tgcaaaaagt atgattgaaa ttgcaagaac 240

acaagacgaa gaagttggag atggtaccac ctcagtaatt gtcttagcag gagaaatgtt 300

agtggcagct gaacagtttt tggaacaaca tattcatccg acaatcataa ttcgccagta 360

cagacaagca ctcgaagata tgattcagtt attggaaggt ccattaagta taccagtcga 420

tagaaacgat aaaaatgctc ttgcccaagt cgtgaaaggc tgcattggca caaaattcat 480

aagtaaatgg tcagatttgg cagtaaatat tgctttagat gcagtaaata cagtattatt 540

agaagaaaat ggcagaactg aagttgatat caagaggtat gccaaagtcg agaagattcc 600

tggaggatcc attgaagaat ctaaaatttt aagaggtgtt atgcttaata aagatgtaac 660

ccatcccaaa atgaaaagat atatacagaa tccgagaata atacttttgg attgtgcatt 720

agaatataaa aaaggggaat ctcaaacaaa cgtagaaatt tcagcagaaa cagacttcac 780

caaactatta gaactggaag aggagcatgt caagcgtcaa tgtgatgaga ttattgcact 840

caaacctgac gtcgtcttca cagaaaaagg agtatcagat ttggcccaac actttttact 900

gaaagctggt attaccgcta tcaggcgtgt tcgaaaatct gataataaca gaattgccag 960

agcctgtgga g 971

<210>745

<211>1049

<212>DNA

<213>Diabrotica virgifera

<400>745

ggaagaggct agaagattgg ctgctcttca gaaacgcaga gaactgagag ctgccggaat 60

agaagttaca tctcgtagaa agaaaaaacg aggagtagac tacaatgctg aagtaccgtt 120

cgaaaagagg ccagcgattg gattttatga cacgtctaat gaagtattag atcctatggc 180

tcccgatttt tcaaaaatga ggcagcaaca cttggatggg gagctgagaa gtgaacaaga 240

ggagagagaa cgtaagaaag ataaacagaa actaaagcag cgcaaggaaa acgacattcc 300

tcaagctatg ttacaaaacc aggaaccagc taagaaacga tccaagttag tgcttccgga 360

accacaaatt tcagaccagg aaatgcacca agtggtgaag ttgggcaaag cgagtgaagc 420

agctagggaa atagttacag aaagcggtgt tgaaaccact gacactttgt tgaatgatta 480

tactttcaat cctcaagcgg ctgctacacc aaggactccg gcgccccaga ctgataggat 540

tttgcaagaa gctcagaaca tgatggcgct cacacacgta gatacgcccc tcaaaggcgg 600

cattaacact ccgttacaca attcagattt cagcggggtc ctgccccaag ctcagaccgt 660

cagcaccccg aataccgtgc ttgctacgcc cttcagatcc agtaggagtg acggagctgg 720

cactcctggc agtagcgttg gatttatgac gcctagaagt ggcgctttgg tgcctgtggg 780

gaagacgccg gtaggaatga cgccttcggt tagggataag cttagtatta atccagagga 840

agcaatggac gtaggaagca caccagctga gcacagaatg taccagatgt cgatgaaaga 900

tcaattaaaa atggggttga gcagtctacc acaaccgaaa aacgactatg aaatcgtggt 960

gcccgaaaat gaagaccagg aagaacccga gcaagctcag tcacaaatga tcgaagatca 1020

agctgatgta gatgcacgga aacaagaag 1049

<210>746

<211>547

<212>DNA

<213>Diabrotica virgifera

<400>746

gatggagtgc taagacaatg ctactcgtgt gtgattgtag atgtttccca cttgatccct 60

gcttctgttt cctagataca tcttgtaatt gggctatttg ttgattcaag ttgctaagat 120

caccttttat gatatatgtg agttcttgga tttctgatgt tttgtcgtca aataaagatt 180

ttcgttttgc caacaatgtc aacttttcca atttagcata agtgctagct atatgccgtc 240

ccacattctt cgcaattagc ataaactcgc tatgactttg tataaccctc gatttcttcg 300

ggtccctagc agctactgcc ctagcaatgt tcctgccttg taatgtctgt atagtattta 360

caaattcact agttctgtct ctggcagtca tgactaattc tggttcagtg tagtcttcga 420

tataatcttc ctctaaatag tagtcagatg taacattgtt tggatacacc gtgtgtgact 480

gattcgtttt aggctgaggg ttagtccaat aattatcgtt acctactata acttggggct 540

cagtttc 547

<210>747

<211>508

<212>DNA

<213>Diabrotica virgifera

<400>747

cacaaattct actatatcct aaagaagcca tcgttaagac tgctgcgaga ccgcaatcac 60

cgactaagaa gcgtggcgga gcacctcaca agcgcctttc caccaacaaa cacgacgtcg 120

ccgtagtccc actacctcga gcgcctttta ccattaccat accaatcacg atttctacgt 180

tttctaacct gcaagataat gtctgctact attttgacgc gaattaattt agcagttcaa 240

aatttgaagc atcatgtact taatactttt ggaagaggtt ttccaccaga tgcactttgc 300

ctagcaggaa tagaattaaa caaaagtcct cagaatttgc ccaaattttc tattaaagat 360

ataataggcg agggattttt atgggcagtt cctaaacata ggcggtcagt agaaaaacga 420

tggaaaagaa aatttggtga tccctattat gtcttgaaaa ttttattgcc tagaaaggat 480

ttaaggacct gtaatgtttg tggtgatg 508

<210>748

<211>788

<212>DNA

<213>Diabrotica virgifera

<400>748

cgtataacct aatcctcttt cttggcatca actttgcctc ccctaatcct accagttctc 60

ctggcagcga taagaccgac tttacgacca gcagatgttc ctctcttaac tgtggatgct 120

ttaccaatgt gttgatggtt accacctccg tgaggatgtt caacagggtt catagcaaca 180

ccacgtacct taggccagca gttacgttta actttgtatt tgtggtaagc acgacctgct 240

tttaagattg gtttgtcaat acgaccacct ccagcaacga tacccaccat ggccctgttg 300

tttgatggaa taactttctt ggctccagaa ggtaacttaa ctctggtttt ctttgtgtcg 360

tgattatggg caattacggt agcatagttt ccagatgcac gagctagccg accacggtca 420

ccagtttttt cttctaagtt gcatacaatg gtaccttcag gcatagttcc tacaggcaat 480

acatttccaa tttgaaggtt agctttcttt ccacagtaca aaaattgtcc agtgtacatt 540

ccttcggggg caataaacaa ttccttccta gtttggaact tgtagggatc tctgaaatgt 600

actactgcca atggagcacc acggccaggg tcatgtatga tatcttttac aacaccctta 660

atgtaaccat gtctttctga gaaatctaaa taacgtagtt tgggtgctcc tttcctcttt 720

tttgtgtggg atttgaatac agatcccgca cctttacgtt gtgcacgaat tacacgaccc 780

attgtaga 788

<210>749

<211>752

<212>DNA

<213>Diabrotica virgifera

<400>749

gaggcgtagg caaggaaaaa ctgattacta tgcccgtaaa cgattgattg ttcaagacaa 60

gaacaaatac aacactccca agtaccgttt gatcgtacgt atctccaaca ggaacattac 120

ttgtcagatt gcttactccc gtattgaggg agacaagatt gtatgtgctg cttactccca 180

tgaattgccc agatatggaa tcaaaactgg tcttaccaac tatgctgctg cctatgccac 240

tggtttactt ttggccagaa ggctcctcaa aaaattggga cttgacaatt tatacagtgg 300

caccactgat gttactgggg acgaatacaa tgttgaagct gctgaacaag gaccaggagc 360

attcaggtgt tacttggaca caggtttaaa ccgtaccagt actggcgccc gtgtctttgg 420

agccatgaaa ggagctgtcg atggaggctt aaacattccc cactctacaa aaagattccc 480

tggttttgac agcgaatcca aaagcttcaa tgctgaagtc cacaggaatc acatttttgg 540

catccatgtt gctgattata tgaaatcact ggaggaggaa gacccagagg cattcaagag 600

gcaattcagt caatacatta agctgggaat taatgctgac cagattgaaa atatgtacaa 660

gaatgcccat gctgccattc gtgcaaatcc tgaattagaa aagaaggctg aaaaggctcc 720

agcacctgca aagagatgga accgtaggaa gc 752

<210>750

<211>436

<212>DNA

<213>Diabrotica virgifera

<400>750

ccacataagg gcaaacttgt tagtattgta gacgtaatcg accagacaag ggttttagtt 60

gatggcccca acagcaatgt acccagagga cagcttaggt taaacgaatt gcatttaaca 120

aagttccgca ttaggtatcc attttcagct ccaactcgtg ttgtaagaaa agcatggact 180

gatgctaaga ttgatgaaaa atggaatgaa tctgtctggg ccaaaaaagt agctgccaag 240

gaaaagagat cacaactcac agatcttgac cggttcaaac ttcgtagggc tcgttcacga 300

agaaaccaca tccgtacagc aactttcctt gctttaagga aggccgcatc aagaaacggt 360

ttcctctatg gtaaaaagaa gatggctaaa ggaggtgaca aacctaaacc aagcaagaag 420

gcgaagaaag aaaagc 436

<210>751

<211>1072

<212>DNA

<213>Diabrotica virgifera

<400>751

tccagttcaa attccgtgcc aaattctacc ccgaagacgt cgccgaagaa ctcatccaag 60

atatcacttt acgcttattc tacttgcaag tgaagaactc cattctctcc gatgaaatct 120

actgcccgcc cgaaacttcc gtccttctgg cttcgtacgc cgtacaagcc aggcatggag 180

attatgataa atcaactcac acgccaggat tcctagccaa cgataggttg ttacctcaaa 240

gggtaatgga tcaacataaa atgtcaaaag atgaatggga gaatagcatc atgacctggt 300

ggaaggaaca tagaggtatg ttaagggaag atgccatgat ggagtacttg aagatagctc 360

aagatctaga aatgtacgga gttaactatt tcgaaatacg taacaaaaag cagacagaat 420

tgtacttagg tgtcgatgcg cttggactta acatttacga aaaagatgac caacttacac 480

ctaaaatcgg tttcccgtgg tcggaaatcc gcaacatctc tttcaatgat aggaagttca 540

tcatcaaacc catcgacaag aaagcacccg atttcgtttt ctttgctcca cgcgtgcgca 600

tcaacaagag aattctcgct ctttgtatgt gcaaccacga actgtacatg cgcagacgta 660

aacctgatac catcgacgtg caacaaatga aagcgcaagc gcgtgaagaa aagatggcaa 720

aacaacagca gaaagaaaag ctccagtctg aaatagcggc aagagaaagg gccgaaaaga 780

aacagcaaga gtatgaagat agaatcaaag ccatgcaaga agagatggaa agaagtcaag 840

ctaactatat ggaagctcaa gagatgatta gacgattgga ggaacaattg aaacaattgc 900

aggctgtcaa agaagagatg gaaaagagac agaacgagtt acagtccatg atggagcgtt 960

tggaagagag caagaacatg gaagccgccg agaggcaaaa attggaagag gagatcaggg 1020

ccaaacaaga ggaggttcta cgtatccaac aagaagtcga ggctaaggac ag 1072

<210>752

<211>864

<212>DNA

<213>Diabrotica virgifera

<400>752

gaatactatg aggacacctt cgccttggtc caaaattcca tgaaacttgg catctaaaat 60

catctgtgac aatttcttct ctacttgtag tactgggagt tttatggttt ttgctacata 120

ctctacttgt accctagaat atggttcgat aattctgcaa aggttctgct ccaacatatt 180

gtcatacaac gttcctaaat gtgctctaac aataacatca tcttctaatt catgtttaaa 240

ctgttttaca gcttgttgga aatctgctag tgatcttttg tgtgcagcct gtgcaactga 300

tttcatagcc tctatatctt gaccagcgta tttaaaggcc aatttaccac taacgatttg 360

ttggacgtct tccggactgt ttatcatgat ttttgacaaa agcatgtatt taagggctgt 420

gagggctttt ggggattcaa cactatcaaa accttcaaaa gcttcataaa aataagaata 480

agctgttttg aaatccttct catctgcagc gtgtaatact ccggattgta aatctaaagc 540

tgcctgcatc tttggagggc aatagatagc gttagctgtg gttcgtgctg aagttagtgc 600

agctctagcc ttcggtaaat tacttaaagc atgatatgtt ttgctttcaa gtagttgtac 660

ttccaccaac aaatttttgt catccaactt ttttaactct ttcagtaagg tcgattccag 720

ttgtagcgct tcgctgtaca ttccagtgtc aaaataaaga gcaatcagcc tggcttcaag 780

ggactgacgt agaaaagttc ttctctcttc ctttgcccat tctatacatt ctttgcagag 840

ttgtacttcg ataccaatac cagc 864

<210>753

<211>383

<212>DNA

<213>Diabrotica virgifera

<400>753

tcatcggtaa acacgaaata attcctagcc atggtgcgta tgaatgtatt gagcgatgct 60

ctaaaatcca taaacaatgc tgaaaagcgc ggtaaaaggc aggtccttat caggccttca 120

tcaaaagtag ttgttaagtt tttaactgta atgatgaagc atggttacat tggcgaattt 180

gaaattgtag acgatcacag aagtggaaag atagtagtaa atttgacagg aaggttaaac 240

aaatgtggag taatttcccc taggtttgat gtaccaatta cacatattga aaaatggaca 300

aataatcttt taccttcccg tcagtttgga tatgttgtgc ttaccacaag tggaggtatc 360

atggatcacg aagaagctag aag 383

<210>754

<211>923

<212>DNA

<213>Diabrotica virgifera

<400>754

gcaataccag gtaaaccaac attctgtctg gcttcaacaa tacgttccat ggcagagttg 60

gctagaccgt agttagcttg gccagcgtta ccacgtccac atgaaactga tgagaagtta 120

acgaaatagt ccaaattgtt cgccatttct ctggaaacca tatctaacat tttgctgccg 180

tcaacttttg gtttgcagac agtcttaaag tcagcttcag attggttttc catcattgcg 240

tcacgcaaga ctactgccag gttaaagatt ccaccaacgg gtcctaattc actggcttct 300

gtaagaagtc ttctggcacc tttgtcagtt gttgcatcgg ctgtagaaac aagtacgttc 360

acaccttttt ctctccatct tctgatacat agtgattggt atccagtttt aattccactt 420

ctggaggtca acaccatttt cttggcacct ctagtaacca accagttggc taattcaaga 480

ccaaatccac caagacctcc aaccaaaatg taactctttt caggatccat gtatgttctg 540

ggaatagcgc tgacagtctt aacagctggg cgagtggcag ttttaggttc ttcttctctt 600

atttggagca aaactttacc aatatgttta cctgaagcca taaatctaaa ggcttgttct 660

acttggtgtt cattgtatac agtctttgga agtggcctta cagctccgct cttaattcct 720

tcagttacca atctcattac ttgtctcttc tgaggactgt ctgaatcaaa gagagcgtca 780

agaaggattc cgtggaaagt agtgttcttc aagaagatgc tcattcccaa cgcagaattg 840

ttagaaagat ctacttttcc aatttccaag aagcgaccac caattgccaa acatcttaca 900

gaagcctgga gttggtgagc agc 923

<210>755

<211>1312

<212>DNA

<213>Diabrotica virgifera

<400>755

cgtggcatga actacgattc tctttcaagt ccgccatgtt gacattcgac aacttttttg 60

ggaggacagg tgaatgttat agcgtttttc aaagtgtaag gtgtttattt tcaaaaagtt 120

tataaaataa gcaatcacta tgggtaatgt gtttgcaaat ttattcaaag gcctctttgg 180

caaaaaggaa atgaggatat tgatggtagg actcgatgca gctggtaaaa ccacaatttt 240

atataaactt aaattaggag aaattgtaac aactattcca acaattggat ttaatgtgga 300

gactgtagaa tataagaaca ttagttttac agtatgggat gtaggtggtc aagataaaat 360

taggccattg tggagacact atctccaaaa cacacaaggc ctaattttcg tagtagacag 420

taacgacagg gaacgtatca ctgaggctaa agatgaatta atgcgtatgt tggccgaaga 480

tgaacttaga gatgccgtac ttctcatttt cgccaacaaa caagatttgc ccaatgcaat 540

gaacgctgca gaaatcaccg acaaactcgg tctccattca ctacgcaacc gcaactggta 600

cattcaagct acctgtgcaa ctagcggaga tggtctctat gaaggtctgg actggttgtc 660

caatcaatta aagaacgcca atcgctagaa cataggaaaa gagaggagtg cgcaaaaaag 720

tgtgcgagtg cagatggttt ttttcttgta ctcttgtcgt gtctactacc tcgcgcgcgc 780

gcgaaacgtt tcgtatacca tatgttgcaa tttttgtgtc cgatggggaa attcgtcatg 840

tttcactttg ggggttataa aattggctgt tggcgacaag tggtgtatga ggtgaatccg 900

aaaatagttt taggcaatat atcatatgaa tctcagcttt ctttaggttg agagttgttt 960

ttgtagtaaa agaaccttaa acatccttca ttggcgattt attatttttt aactgcatga 1020

aaatcttagt tttaatgttt ttatataaca cttttagatt gtactaactt ttaacattcc 1080

acaaatttct cataaaattg tgatatcttt tctaatacct cttatatcaa tgaacttatt 1140

tttgcaagcc aaaatatttt cttagaaagt aattttgtat catctatata aatttaattt 1200

tgggaattca attaaagact ttaactggca aattttaaaa tgttaaaggt tctagtcacg 1260

tatttagtct ggacttgcac tttatagcac ttgtcgcaga aagcatctaa cc 1312

<210>756

<211>442

<212>DNA

<213>Diabrotica virgifera

<400>756

taggttgggc aggttgtctc ctaagttcta ctttaacgtt cttttcttta gcttctttac 60

gtagcttttc gttggatttt actctttgca agaagtcttg acgacacttg gagtggttta 120

catgttcaat acggagattg attcttttgg ggatgattct tcctcgaacc cttttgttta 180

caattacacc taatgcatgt gcagtaacgt tgaaaacacg tcctgtctta ccatggtaca 240

ctttgtgggg catacccttt tgaactgcac cattaccctt gatatctaca atatctccaa 300

ctttgtagac tctcaaatat gtggaaagtg gaattacacc acgtttttta aacttgcggg 360

caaatagatc cctggttcct cggcggtaac ctttagagtt ggtcattttg taccttccaa 420

atcctaagaa acacagtaaa cc 442

<210>757

<211>404

<212>DNA

<213>Diabrotica virgifera

<400>757

gtgcgagaac ttcactatct attcaacatg cagttgtgtt taagaggaac cgcaactcat 60

ttccttgaat gccaaggatc tgagactgtt ggagaaatta aggaccgtat tgcatctttg 120

gaatcgctta aagcatccga aatcagtctt tatgctgctg gagttccagt ttctgaggaa 180

aggttggtag ccgattttga gaacacagac attgaattga ctgttggctt gcctggtggt 240

aaagttcacg gatctttagc tcgtgcaggt aaagtaaagg gacaaactcc aaaagtagaa 300

aagcaagaaa agaaaaagaa gaagactggt agagctaaga ggcgtattca atataacaga 360

agatttgtta atgtagttgc atctttcgga cgcaggaggg gacc 404

<210>758

<211>747

<212>DNA

<213>Diabrot ica virgifera

<400>758

gtcatctcag tttggtttat tgttgggtgt aatgtttaaa taaaataatt attattttgt 60

aaacctgtgc cattaaacaa ataaagcgat ggtgctaatt gcagcagcag tctgcacgaa 120

agcaggcaaa acaattgtgt ctcgacaatt tgttgaaatg accaaagcta gaatagaagg 180

tttgttggct gcctttccta aattaattcc tacaggaacc cagcatacat ttgtggaaac 240

agattcagta cggtatgttt atcaacccct agagaaactg tatatggttc ttattacaac 300

tagagctagc aacatattag aagatcttga aaccctccgt ctattcgcaa gagtgattcc 360

tgagtactgc aaatctttgg atgaaaatga aattgcagag aatgcatttt cacttatatt 420

tgcttttgat gaaatagtgg cattaggtta tagggaaagt gtcaatctgt ctcaaattcg 480

cacatttgtg gaaatggact cgcacgaaga aaaagtttat caggccgtga gacagactca 540

agagcgcgag gccaagaata tgatgagaga aaaggcaaaa gaacttcaga gacagaagat 600

cgaagcggcc aaaaaaggag ggaagacctc gtttggtagt agcggtggct ttggcagctc 660

gacaggttat actcctacgc catctgttgg tgatgtagct aaccagacaa atgatgttaa 720

aacttcttca tacacaccag cccctgc 747

<210>759

<211>1285

<212>DNA

<213>Diabrotica virgifera

<400>759

gcactgattc gtttatttca cctagaagat tgaaagccga ttctactcca tcatccgcaa 60

cctgattgtt atctctggct ttttgaactt catcagaatc ataagaaaga ataaagtagc 120

tatcttctgt ggccaaacat actaatttac cactatctga ccagtaaact gcttttggtt 180

gtatctcgat tcttctgact aaatcgagag tttcccaatc atagaaagtc aaaccagaaa 240

ccgatttgac tcccaaaagg tatccaccgt atataccttc agctccaaaa tcggacttaa 300

aattcttctt ctctttgaaa ttcttaaaaa ttctgatagt agatccggat tctctgatgg 360

catattcgct ggaatcttga gcccacacaa attcttgtgc gctaccaaac gctttgtttc 420

ttaaagccat tgctgtgtag attatgtatt ctccatcccc acagacaaca acaaaacggc 480

cattgggatt gtgttgaatt gtctgagggt atatctcgca agcacccata tcttttacag 540

aaactggaag gcgttctcca tctcttattt ccgcaccttc agctaacgcc ttgagatttg 600

cctgttgaag ttcagagtgt ctggcccaaa taattttgcc tccactggca tccatactaa 660

cagctggttc ttctctacca actttaacca aaatgctacc ttcatcataa cccaatgcca 720

cgttattgga tccctttagg cagaaaatag tccatactct ttcaaagcca taatttaagc 780

tactttctaa cctatgggtg ttggcatgcc acactctgac agtaccatct tcacttccag 840

taagagctac aggaagttct ggatggaaac atgcagcggt tacattttga gcatgtcctt 900

ccaaagtttg aacacaagtt ttgttttgat aatcccagat ttttactaat ctatcatcag 960

cgcctgagat taaataaggt ttatctccac cgtgataata gtccacacag ttaacgcctt 1020

tctcatgacc ttctagtgtg aaattcgctg tggacgctcc caattgccat actttcaatg 1080

ttctatctag ggatgcactg gcaaatgtgt tgttgtcttt tggatttatg gcgatttgca 1140

taatataatg agtgtgtcct tcgaaaactt gctgacaagc ccatgctttt tcccaattcc 1200

aaagcttgat aagcatatca tcactacttg ttaatatata aggttgtgta gggtgtacga 1260

caatacatct cacataatcc gaatg 1285

<210>760

<211>442

<212>DNA

<213>Diabrotica virgifera

<400>760

ggaaaacaac ccaaagaaga gtccaactat ctcttggacc tcaagttcgt gaaggtgaaa 60

tagttttcgg tgtagctcac attttcgcca gtttcaatga tacatttgtt catgttactg 120

acttgtctgg tagagaaact atctccagag tcactggagg tatgaaagtc aaagctgaca 180

gagatgaagc ttctccctat gctgctatgt tagcagccca ggatgtagct gaaaaatgca 240

aaacattagg aatcactgca ctccacatca aattgagggc tacaggtggt aacaaaacca 300

aaacccctgg accaggagcc caaagcgcac tcagagcttt ggctcgttca aacatgaaaa 360

ttggtaggat tgaagatgta actccaattc catctgattc cacacgtagg aagggaggtc 420

gccgtggtcg tagattgtag ac 442

<210>761

<211>959

<212>DNA

<213>Diabrotica virgifera

<400>761

cgatgttctg ccgtgactgt ctcagtctgt tttagactaa cagagacagc cacggcaata 60

ttcctttcca atacccactc tcctgatcaa cgggtggctt taatgcttta tctaatcctt 120

ctattctgtg gtatccatgg cagaagtttc cacagctggg actgcatccc tgggctcgtc 180

ccccgcatca tctctcaacg gatctgcagg aaggtcctca attgcagcag ctgcagctcc 240

tcctcctcta cgttcttcac cctcaataag ggacaagtag ggggaagttt cttcttcttc 300

gaggatttta aaggtctgac ctttgccagt gtatcctatg gagacatttt ttgttgtgag 360

gtcaacctct ggaggtagag tatctctaag agccctcaat ccatgtttga taagttcttc 420

taaagaacta gatggcaatt catccagatg tttttctaag tatgtcctgg cactctggga 480

tcgagctcca atggacatag ctttgcaatc gtagaaatta gcagaagggc acgtttggta 540

gatatgagaa ccagattcgt cataaccagc tactagaaga cctacgccaa atggtctgcg 600

gtcgtatctt tgggtacatg tctgcatttt gttacctaaa atactgataa gcctattcaa 660

tggcatataa gtatccaaag agtacttata attcaagcat tcgtttcgca tatatctact 720

aaggatccta gcatcagctg taagtcccga gatggtgata ccaatgtgat cgtctatgtt 780

gataattttc ttctggtagg cagacagttc tgaagaagcc ctcttcaaag cgataagtac 840

agcatgagtt ttacttttca gtcccactgt ggctgaacct aggttaactg cttccatggc 900

atattctacc tgatgcagac ggccctgggg gctccacact gtcacatcac tatcgtatt 959

<210>762

<211>429

<212>DNA

<213>Diabrotica virgifera

<400>762

ccttggcggg ggctgtagcc ttcttggctg tctttgtgct ctttttctgt tccttagcgg 60

cacgaatggc ttgctccctt tgggccttcc ttacttcagg cctttggttt ctcttggcca 120

tgatatcatt taaagaagca cctacaatag ccctttggaa tttctgtgtc cttctggtac 180

gttttttggt ggcttcttcc tcctgaccct tcttatgttt acgtctgtac aagacagtcc 240

atgttacttt acgggggttc cttttcatca agtgcgccct ttcacatttt gagttgagga 300

aagtgaaggt ctttccatca actttaacca ttgttttgcc atgtccgggg taaattttgt 360

acccgctgta agcacaaagt ccgatcttca ttttggaatg aaattactac ttaaatgaca 420

gatgacaac 429

<210>763

<211>828

<212>DNA

<213>Diabrotica virgifera

<400>763

cagtgacagg acggtgattc ttagccaagt agtcagcaaa ctcttggtat ggtgtcttag 60

ccaaagccat atccttccac aaatcaggtg tcaagtaagc ataggtcttt gcgatggctg 120

cgtatgtagc tttggcaaag ttacctaaag taccagtgga acctctagcg gatgtgtagc 180

aatcttcaat accagccatc tgcagaagtt tcttaggaac aggggcagaa acgattccgg 240

taccacgggg agctgggatc aaacgcacag taacagaacc acattttcca gtaaccttgc 300

aaggtacagt gtggggttga ccaatcttgt taccccagta tcctcttcgt actggaacga 360

cagacagttt ggctaaaata atagcacctc tgatggcagt tgctacttcc ttgctgcatt 420

ttacacctaa tccaatgtgt cctttgctat ctccaatggc aacaaacgcc ttgaaacgtg 480

tacgttgacc agcacgagtt tgcttctgga caggcatgat tttcaatacc tcatcattca 540

agtctgatcc aatgaaatga tcgatgattt cgaattcttt gattggcaaa gagaacaggt 600

agatttcttc aagagaacgg atctttccat ccttgactaa acgtcctagc ttggttactg 660

gtacccattc tttttgatct tcctttccac gaccacgtcc tctgccacga cctctacctc 720

ggcctctggg accacctcta ccgcctccac ggccaccacc tccaaaacct cctcggaatc 780

caccacgtcc accggctgga gctgcgtccg ccattgttta gttccttc 828

<210>764

<211>664

<212>DNA

<213>Diabrotica virgifera

<400>764

ggcagacgct gatgatctat tagattatga agatgaggaa cagacagaac aaaccgcaac 60

tgaaacggca actacagagg tacagaaaaa gggtgtcaag ggcacatatg tatcaataca 120

cagttctggg tttagagatt ttctgttaaa accagcaatt ctcagagcta tagtggactg 180

cgggttcgaa catccttcag aagttcaaca tgaatgtatt cctcaagctg tcattggcat 240

ggatattctg tgccaagcta aatccggtat gggaaaaacg gctgtttttg tattagctac 300

actccaagta atagatccta cagaaaatgt tgtatatgtt ctcgtcatgt gccataccag 360

agagttagcc ttccagataa gcaaagagta cgaacgtttc agtaaatata tgcccaatat 420

taaagtaggg gtcttctttg gtggcttgcc tatccagaaa gatgaggaaa cgttaaaaaa 480

taattgcccg catatcgttg tgggtactcc aggaagaatt ttagcattgg tcagatcgaa 540

aaaacttaat ctcaaacatc taaagcattt tattttggat gaatgtgata aaatgttgga 600

gttattagac atgagacgtg atgttcaaga aatatatcgt aacactcccc acgaaaaaca 660

agtc 664

<210>765

<211>434

<212>DNA

<213>Diabrotica virgifera

<400>765

caagagagga cgtggtggtt ccgcgggagc gaagtttagg atctcccttg ctcttcctgt 60

aggagcagta attaattgtg cagataatac aggagcaaag aacttatatg ttatcgctgt 120

acaaggtatt ggtggacgac ttaaccgtct gccagctgca ggatctggtg acatgattgt 180

agccacagtc aaaaagggta aaccagaact caggaaaaaa gtcatgcctg cagtagttat 240

caggcagcgg aaaccattta gaaggaagga tggtgtcttc atttactttg aagataatgc 300

tggggttata gtcaataaca aaggtgaaat gaaaggatca gccatcactg gtccagttgc 360

aaaagaatgt gcagatttat ggcccagaat tgcatcaaat gcaagcagta ttgcttgatg 420

tgtatatgtt taag 434

<210>766

<211>1283

<212>DNA

<213>Diabrotica virgifera

<400>766

ccaccctcag gcgtaggtat gaatgcaggc gttaatttga gtggcatttg cattttcttg 60

cttatactta gcctatagtt ccttaaaact gtcatgatac ctacttttac ttgaatgatg 120

ccaaacctca tacctaaaca taacctaggg ccttctccaa aaggaacata tgtgtatgga 180

ataatgttct ttctgttctc ttctgaaaat cgttctggat cgaatacatc tggattatca 240

taatattctt catcatagtg aatacctatg gtaggtatga cgactcttgt gcctttttca 300

actactatgt ttgtctcagg tatctggtaa tcttgagaac attctctggt gagaaaagga 360

attggtggat atttccttaa tgtttcatcg atgcattgac ccatatattt aagatcgcag 420

agggactcgt aggtgatttc gccgttgtga ttgtttagta cttcttctat ttcttctcta 480

actctttctt ggatgtcggg attggcagcc atctcgtata aaacccatga catagtggtt 540

gcgctggttt cgtagcctgc tacgaaaaac acgaaacttt gagccactat ttcgtccatt 600

gtgatagctg tctcgtcgtt taattttcct cttgtttctg gatcgttctt aatattgatc 660

agtccttgta agaaatcatt acgatcaatt ttactatttt ccctatacgt aacagtatca 720

acaaactttc gtgaaaaatt ccttaatagt cttcggtatc caagtcaatc tgatagcact 780

agctaatttt ggaaagttga ttgcaacagc aaatttgacg tcatccgaaa gtgttctgtt 840

tacgattcta ttggccatct gcttgaacgg attgtcatct tcgaaactgt tgcagtctat 900

tccaaatgcg catgaaccta ttacgctgat ggtgaaacta cccataaatt gtttaagatc 960

aacgggctcc tctccattaa tctttgtgct taagcaattc tccaacattt ttccacatga 1020

aatgacagtg tgaaacatcg attttaactt tcccgatgaa aaatttggcg taaatttact 1080

tctaaggctc ttccattttg tcccaccaag tgcaaataag tgagcgccta taggttcatc 1140

tttttcattg atatatattc ctcgggcatt aaagtaatta aaatccttgg ctaaaacccg 1200

ctttataaga tcattatcta ctggcatata aattggagat atagcggcat aaagtccgat 1260

gtgtttgtgt ccatctcgct tag 1283

<210>767

<211>685

<212>DNA

<213>Diabroticavirgifera

<400>767

cagactacgg ctcacacgag agtaggttgt acaaacatga tttacaacaa ttttgcacta 60

atttcaatag taacatgaat ctagaagtgc ccccctttga aagtcctgcc accaacttat 120

ctagatttac aaaagatgat actggaaagg ctctaaaaat taattttgat catggtacca 180

caaccttggg attcatgtat cagggaggag ttgtacttgc agtagattcc agagctactg 240

gaggccagtt tattggttca caaacaatga agaaaattgt agaaatcaat gattttctgt 300

taggtactct ggctggaggc gcagcagatt gtgtctattg ggacagagtt ttggccaagc 360

agtgtagaat gtatgaactc agaaatagag aacggatttc agtagcggct gcttctaaat 420

taatggcaaa tatggtttac aattataaag gaatgggatt gtcaatgggt atgatgttag 480

ctggatggga caaaagggga ccacaattgt attacgtgga ctcagaaggt ctgagaacag 540

caggaaaagt tttcagtgtt ggttccggttccatatatgc ttttggtgta ctagactctg 600

ggcacaaatg ggatcttacg gacgaagaag catatgattt gggccgtaga tcgatttatc 660

acgctaccca cagagatgct tactc 685

<210>768

<211>859

<212>DNA

<213>Diabrotica virgifera

<400>768

gcgtactaag ggacccgaca cagctttaaa actaccagcg gttactcctc atacaagatg 60

ccggttgaaa ctattaaagt tggaaagaat aaaggattat ttgcttatgg aagaagagtt 120

catcagaaac caagaaaggc tgaaaccaca agaagaaaaa aatgaggagg agagatcgaa 180

ggttgatgat cttagaggaa ctcccatgtc tgttggtaca ttggaagaaa tcattgatga 240

caaccatgcaattgtttcca catcagtagg cagtgaacat tacgtgagca ttttatcctt 300

tgttgacaag gatcaacttg aaccaggatg ttcagtactt ttgaaccata aggtgcacgc 360

cgttgtaggt gttttaggtg atgatactga cccaatggta acagtaatga agcttgaaaa 420

agctcctcaa gaaacctatg ccgatattgg tggtctagat acacagattc aagaaattaa 480

agaatctgtt gaattacctc ttacccatcc tgagtattat gaagaaatgg gtatcaaacc 540

acctaaaggt gttatccttt atggccctcc aggtactgga aaaactcttc tggcaaaagc 600

tgttgctaac cagacttctg caacattttt gagggttgtt ggttcagaac ttatacaaaa 660

atatttgggt gatggaccta aactagtccg agaattattt agggtagctg aagagcatgc 720

tccttccatt gtatttatag atgaaatcga cgctgttggt acgaagaggt acgattccaa 780

ttctggcggt gaaagagaaattcagagaac tatgttagag ttattgaatc agttggatgg 840

attcgattca aggggcgac 859

<210>769

<211>586

<212>DNA

<213>Diabroticavirgifera

<400>769

cgtcggttca ttctaaaacc tatgtaacgc ctaggcgtcc atatgagaag gcccgtttag 60

atcaagaatt gaaaatcatc ggagcctttg gtctccgaaa caaacgtgaa gtgtggagag 120

taaaatacac tctagccaaa atccgtaagg ccgccagaga actgcttact ctagaagaga 180

aagaacccaa aaggttgttt gaaggtaatg cactcctacg tcgtttggtt cgtattggag 240

tactagacga gaacagaatg aagctcgatt acgtattggg tctcaagatt gaagatttct 300

tggaaagacg tctgcaaaca caagtcttca aatctggtct agctaagtca atccatcatg 360

ctagggtatt gatcagacaa cgacacattc gggtccgcaa acaagttgtg aacattccct 420

ccttcatcgt ccgtttggat tcacaaaaac acatcgactt ctctctgaaa tcacctttgg 480

gaggcggtcg tccaggacgt gttaagagga agaacctcac caagaagagc ggtggtggag 540

gtgcagctga agaagaggaa gactaagctg tatgtcgtgc tagttg 586

<210>770

<211>563

<212>DNA

<213>Diabrotica virgifera

<400>770

ggatagactt cttcgactag aggtggaatg ccaggtttaa ctcaggcacc gccgccttct 60

gatgctcctg ttgtggatac tgccgaacaa gtatacatat cctccttagc actattaaaa 120

atgttgaagc acggccgagc tggagtaccc atggaagtaa tgggtcttat gttaggagaa 180

ttcgtagatg actataccgt tcgtgtaatc gatgtatttg ctatgcctca gactggtacc 240

ggtgtcagtg tagaagctgt tgatccagtg tttcaagcaa aaatgcttga catgttaaga 300

cagactggta ggccagaaat ggttgtgggg tggtatcatt cccatccagg tttcggatgt 360

tggttgtctg gagtggatat caacacccag caaagttttg aggctctctc ggaaagagct 420

gtagcagtag tagttgatcc aatccagtca gtgaaaggca aagtcgttat tgatgccttt 480

aggttaataa atccgaatat gatggtattg ggccaagaac ctcgccaaac tacatccaac 540

ttaggacatc tacagaaacc atc 563

<210>771

<211>1216

<212>DNA

<213>Diabrotica virgifera

<400>771

cctgatttag aattagcaca ttgtaagttt ctgttaactt taccagaata tcgcagtgat 60

aaaaacgtcc actccaaaat aacagaagct ataaagaaag atgatatggc cccatggtat 120

gaacttattt gtaaagatgc tggttggaaa cttgatgcta atctactgag tgcattaaaa 180

gctaagaacg ctgagaagat caagcttttg gatgaagcca tagaagatgc cgagaaaaat 240

ttaggtgaaa tggaagttcg agaagcctac ttaagaaaag cagaatacta cagccgaatt 300

ggtgataaag acaacgcagt tagtactttc agacaaacat atgataaaac tgtatcactt 360

ggtcatcgtt tagatattat ttttcacttg ataagaattg gtttgttctt catggaccat 420

gatctcatca caagaaatat tgaaaaagct aaaaccctta ttgaagaagg aggtgattgg 480

gatagaagaa atcgtttaaa agtgtatcaa ggtgcatatt gtatgtcagt acgagacttc 540

aaatcagctg ctaacttgtt tttggacaca gtaagcactt ttacatcata cgaactaatg 600

gactataaag catttgtcag gtatactgta tacacctcta ttattagttt gccaaggaac 660

caattaagag ataaagtggt caaaggttcc gaaatattgg aagtactgca ttcagaacca 720

tttgtaaaag attacctttt ttctttatat aattgtcagt atgctgaatt tttcacaaat 780

cttgcagaag ttgaaacagt tctcagaaaa gattatttct tgaatcctca ctatcgtttc 840

tatgtaagag agatgaagat acttgcttat acacaattat tggagtcata taggtctctt 900

actttacaat acatggcaga agcctttgga gttactgttg aatacattga taaagaactg 960

tccacattta tagcagcagg aagattgcat tgtaaaattg atcgagttgg gggaattgtt 1020

gaaactaata gaccagattt aaagaatgcc caatttaatt ctgtggtaaa acaaggagat 1080

ttactgctga atagagtcca aaaattatct agggtgatca atatctaaaa aatgtattct 1140

taagtttata agtaaattat aaaacatact gataatttga cttgattgta ctaattttct 1200

atgtttatct agtgct 1216

<210>772

<211>617

<212>DNA

<213>Diabrotica virgifera

<400>772

cctctggaaa gttggttcag attgaatatg cattagccgc tgtagcggcg ggtgctccat 60

cagtaggaat taaagcatca aatggtgtgg ttattgcaac agagaacaag cacaaatcaa 120

ttctgtatga tgaacacagt gtccataaag tagaaatgat cacaaaacac attggtatgg 180

tttattctgg tatgggtcct gactatcgcc ttcttgtgag acaagcacgc aagatggctc 240

agcagtatta cctagtatac catgaaccca ttcctacagt acagttggtg caaagagtag 300

cgactgtaat gcaagaatac acccaatcag gaggagttag accatttgga gtgtcgctac 360

tgatttgtgg ttgggataac aacagacctt acttgttcca atgcgatcct tctggagctt 420

actttgcttg gaaagctact gcaatgggaa agaattatgt caatggaaaa acgttcttag 480

aaaaaaggta cagtgaagag ctagaactcg acgatgctgt gcacactgct atccttaccc 540

tgaaagaaag ttttgaaggc cagatgacag ctgacaacat agaagttggt atttgcgatg 600

ctaatggatt cagaagg 617

<210>773

<211>736

<212>DNA

<213>Diabrotica virgifera

<400>773

gtctcagcct gaagcaactt tccttaaaga attaacatat aggagtacca atacaaaaga 60

accaggagta gtaactgctc cctcgtctaa ccttaataca gcctttcgtc taattaaaga 120

agttcaaagg aaatttaaga cacgagaagc tgaagaaaaa gaaaaagagg atttggttaa 180

acagggcaca ttagtacttt cacagaataa aggaaatcct aaattaaaag atctgtatat 240

taggccaaat attgtttcaa aacgtatgac aggatcatta gaagctcaca ctaatggttt 300

taggtatact tctgtgaggg gtgataaagt agacattctt tacaacaaca tcaagaatgc 360

cttctttcaa ccgtgtgatg gagaaatgat aattctgtta cattttcacc taaaacatgc 420

tatcatgttc ggaaaaaaga aacatgttga cgttcaattc tgtacggaag tcggagaaat 480

taccacagat ttgggcaaac atcaacacat gcacgatcga gatgatctgg ctgctgagca 540

gtcggaaaga gagctcagac aaaaattgaa aactgcattc aaaagttttt gtgaaaaggt 600

tgaatctatg acaaaacaag aaattgaatt tgatacacca ttccgggagt tgggatttcc 660

aggagccccc ttccgctcta cagtccttct ccagcctaca tctggttgtt tggttaacct 720

gactgaatgg gcctcc 736

<210>774

<211>718

<212>DNA

<213>Diabrotica virgifera

<400>774

gactggtacg atgtgaaagc tccatcaatg tttgccaccc gccaggtcgg caggactttg 60

gttaaccgta cccaaggaac caaaattgcc tctgaaggtt tgaaattcag agtttttgaa 120

gtgtctttag ccgatttaca aaatgataat gacgccgaaa ggtctttcag aaagttccgt 180

ttgatagctg aagatgttca aggtagaaat gttttaacaa atttccacgg tatggattta 240

accactgaca agctgaggtc catggttaag aaatggcaaa ccttaattga agcatctgct 300

gatgtcaaga catcagatgg atatttactt agggtgttct gcataggctt taccaacaaa 360

gaccaacttt ctcaaaggaa aacatgctat gcacaacata cacaagttag agctatccgt 420

aaaaagatgg tagacattat taccagagac atccagggct cagacatcaa agaagtagtg 480

aataaactcc ttcctgacag catagccaag gatattgaaa aggcctgcca gggtatctac 540

cccctacatg atgtgtacat ccgtaaagtt aaagtattga aaaagcctcg ttttgaactc 600

tccaaattgt tggaattgca tggtgatgga ggcaaaggtg gtgctggtga ctccactggt 660

gaagctggtt ctaaagtcga caggcctgag ggatacgaac caccagtaca agaatccg 718

<210>775

<211>598

<212>DNA

<213>Diabrotica virgifera

<400>775

gttgagaggt gcatttgcag gatgagttcc ttaaaacttc agaagaggct agcagcctct 60

gttatgcgat gtggtaaaaa gaaagtatgg ttggacccta atgaaatcaa cgaaattgcc 120

aacactaact caagacagaa catccgtaag ttgataaagg atggtcttat tattaagaag 180

cccgtagctg tacattcccg tgcccgtgtt cgcaaaaaca ctgaagcccg caggaaagga 240

aggcactgcg gttttggtaa aaggaagggt actgctaatg cccgtacccc acaaaaggaa 300

ttatggattc aacgcatgag agttttgcgt cgtctcctta aaaaatacag ggaagctaaa 360

aaaattgaca gacatctata ccactcactc tacatgaagg ccaagggtaa cgtattcaag 420

aacaagcgtg tccttatgga atacatccac aagaagaagg cagagaaagc ccgtgccaag 480

atgttggcag accaggccaa tgccagaagg atgaaggtaa aacaggctag agaaagacgt 540

gaggaacgta tcgccacaaa gaaacaagaa gttttgcaga actacatgag ggaggatg 598

<210>776

<211>683

<212>DNA

<213>Diabrotica virgifera

<400>776

gagatagaag agccctacag ccagatgcca tgtattatcc agacttaatg cctcccccta 60

gttatcctga taatcctatt aattgtgtta ctactagatt tgtaaaaact gctacaaata 120

aaatgaggtg cccaattttc tgtatggcct ggacacccga gggcagaaga ctggtaactg 180

gtgctagttc aggagaattc acattatgga atggattaac atttaatttc gaaactatct 240

tacaggcaca tgacagtcca gtcaggacga tggtatggag tcacaatgac agctggatga 300

taacaggtga ccattctggt tatgtcaaat attggcagtc gaacatgaac aatgtgaaga 360

tgttccaagc gcacaaagaa gcacttcgag gcataaggta tctacaactt ctctccgtaa 420

ttataaaact tgtacactta cttatatttt actttatcaa taaaagatcc ctccttctcc 480

ctacattagt gcaaaataaa gaaatcaatt ttaaataacg ccatcaatat tttccaaaca 540

atacattgca tttcaaattg tctagttgga aacattgtac ttaactgact ctattagagt 600

ttctgaatag accatacaaa actaattccc ttaaagtaag atggtgaatg gacattgatt 660

ccgaaggcaa cagtccccaa aca 683

<210>777

<211>321

<212>DNA

<213>Diabrotica virgifera

<400>777

ccacgcgtcc gttttatagt aaagccagca tggcaccaag gtacgagatc gcagtcggcc 60

tccaaagggg tcacaaaacc accaaaatct cagacaaatc caaactgaat aaagttcgtc 120

ccgctaggtt gaaggggatc caaaccaaac acaccaaatt tgtcagagac atcatccgtg 180

aagtcgtagg acatgctcca tatgaaaaga gagccatgga attgttaaag gtgtcaaagg 240

ataagagggc cctgaaattc ctgaagcgtc gtcttggcac acacatcaga gccaagagga 300

agcgtgaaga actatccaac a 321

<210>778

<211>311

<212>DNA

<213>Diabrotica virgifera

<400>778

ccagccccac aagtacaatc tggtgggccc atggacgtaa atcaagcact ccaagaggtc 60

ttgaaaacag cccttattca cgatggagtc gttcatggtt tgcatgaggc tgctaaagct 120

ttggataaaa gacaagccgt actttgtgtg ctcgctgaaa actgtgacga gcctatgtat 180

aagaaactag tctcagcttt atgctcagaa catcaaattc ctcttattaa ggtagacaat 240

aacaaaaaat taggcgagtg gtcagggtta tgcaaaattg ataacactgg taaagcacga 300

aaggtcgttg g 311

<210>779

<211>501

<212>DNA

<213>Diabrotica virgifera

<400>779

gtgccagtgc ttaattttcc tcgatgatcc attgtctgtg gctgaaattt tagacaggct 60

tgtaactggc aaggaggata ctgaacttat ggcatatcaa attgcttttg atctctatga 120

gtcagctact caacagtttt tggaaagagt attagttgca ttaagagcca cagcaccagt 180

tccatctttg ctggaagaaa aagcaaagcc aaagccttct ggtgaaggag agggtgctat 240

gtcaaccgag actggagaac catctacaga aacaaaagaa gaaaaattaa aagaagaaaa 300

atctctggat agtttgtctg atcaggataa ggaacatcag aaaagaattg agaaacttca 360

tactatactc tcaggggagg tctctataga actgcacctt caattcctca tcaggtcaaa 420

tcatgctgat ctactgattc ttaaacaaac aaaagagact gtcagagtta gtatttgcca 480

cactgctact gtaatcgcaa a 501

<210>780

<211>370

<212>DNA

<213>Diabrotica virgifera

<400>780

ttgccgttca aaatgccatg cagcatttaa aaagaaaaag aatccacgta aagtaaaatg 60

gaccaaggcg tacaggaaaa cagtaggcaa agagttaaca atagatccat catttgaatt 120

tgaaaaacgt agaaacatac cacttaaata taacagagaa acctggtcaa aggctgtaga 180

agcaatgaaa acggtagaga aaataaaaca gaaaaggcaa ggagcctaca tcatgcaaag 240

attacgtaaa ggtagagaaa tcgaacaaga acgagacatc aaagaggtac aaagggatct 300

gtctcttatc agatccccag ctgttggact taaagaaaga aaacaagaag aatctatggc 360

gcaagatcac 370

<210>781

<211>673

<212>DNA

<213>Diabrotica virgifera

<400>781

ttggagacaa gttcgcgtct aggcacggac aaaagggaac gtgcggtata caataccgtc 60

aagaggatat gatttttagt gctgaaggta ttacgccaga cattatcatt aatcctcacg 120

ctatcccctc ccgtatgacg attggtcact tgatcgagtg catccaaggt aaggtatcat 180

ccaataaagg agaaatcggt gatgctacac ctttcaacga tgccgtcaac gtacagaaaa 240

tctccacttt gttacaagaa tatggttatc aactcagagg caacgaagta atgtacaacg 300

gccatactgg acgtaagata aatgctcaga ttttcttagg acctacctat tatcaaagat 360

tgaagcacat ggtggacgat aagattcatt caagagctag aggaccgttg cagattctcg 420

tcagacagcc tatggagggt cgtgcaagag acggcggtct acgtttcggt gagatggaac 480

gagattgtca aatttcacac ggtgcggcac aattcttaag agagaggctg tttgaagttt 540

ccgatcctta tagaatccat atttgcaact tctgtggttt gatagctatc gccaatttgc 600

gtaacaacac ctttgaatgt aagggttgca aaaataagac gcagatatcg caagttagac 660

tgccttatgc tgc 673

<210>782

<211>411

<212>DNA

<213>Diabrotica virgifera

<400>782

tagtaactgt aaaaggaccc cgaggtaatc tgaagagatc cttcaaacat cttaccctgg 60

acattcgaat gatcaacccc aggttactga aagtagagaa atggttcggt accaagaagg 120

aattagctgc tgtcagaaca gtatgctctc atgttgaaaa catgttaaag ggtgttacaa 180

aaggatacca atacaagatg agagcagcat atgctcactt tcccattaac tgtgtcacca 240

ctgaaggaaa cacagttatc gaaattagaa atttcttggg agaaaaatac atcagaagag 300

taaagatggc cccaggtgtt acagtagtaa actccaccaa acaaaaggat gaacttatca 360

ttgaaggaaa ctcattggag gatgtttcaa agtcagctgc ccttattcaa c 411

<210>783

<211>553

<212>DNA

<213>Diabrotica virgifera

<400>783

agagaccatt ggcataaacg aaggtccaca ggaggcaaga ggaagcctct ccgtaagaag 60

aggaagttcg aattaggacg tccagccgca aataccaaac ttggagcacg tagaattcac 120

tatgtccgta cacgaggagg taacttgaag taccgtgcat tgaggattga aaaaggaaat 180

ttcgcatggg gcagcgaagg tactgctcgt aaaacacgta ttattgatgt cgtttacaat 240

gccagtaata acgaattggt acgtaccaaa acattggtaa agaatgccat cgttgttatt 300

gacgcaaccc ctttcagaca atggtacgaa agccactata ttttaccttt aggacgcaag 360

aagggggcaa aattgacagc ggaagatgaa gaaatgttca ataagaaacg tagcaaaaaa 420

gtacagaaaa aatatgaaac taggcaaaaa acagctaaag tagaacctgc attagaagaa 480

caattctcaa ctggaaggtt attagcttgc ttagcgtcaa ggccaggaca atgtggtaga 540

gctgatggtt acg 553

<210>784

<211>902

<212>DNA

<213>Diabrotica virgifera

<400>784

gacccatgaa tgcagctttg tcagcagctg tctggaatct accatgaccg aatttagatg 60

aggtatcaat gaatttaaga ttgatcttct ccaaagcaat acgtttagtg tggactaaca 120

atgacttcct caatgtaata acacgtttct ttggtcccat acaacgtcct ttaatcataa 180

caaagtcatt gttaacttca ccataatgag gaaaaccacc cattggggta atagtttttt 240

cactaagatc gtaggcagtg gctgcattat ttttgacaac cttgccatct ttagtgtgga 300

ttccagcacc aattctgtag atctttttgt tcatctcagt tctgtgatgg taaccctttt 360

gaccagcacg agcaactgtg aatgatactc ttgaaggatg ccatgctcca atacaggcaa 420

ctttacgaag acctttgtgt gtcttacggg gcaatttctt tgtgtgccaa cgagaagtaa 480

cacctttgta tccatgaccc ttagtaacac caatgcaatc aatcatttca tcttgagcaa 540

aaacgttggc aataggaata ggcttttcaa ggtgttcttt agcccaagct actttgtcag 600

ctacactacc accattcaat tgaatttcca taatgtgggc tttcttttga cgttgtttca 660

acaatttcat ctgagtatga gcaataactc tgacaacttt acagtattta acaatcttct 720

ttaagtcctt ttcgatggac ttctttccta aatcatcact ccatttcttg gaagctttgg 780

taaatgcctt cttcttgctc ttgtaccagt tcttgtagaa gcgcctgcgg caatcttcag 840

agagatgctc agcccaaact gtggctaaag ccctaagacc atgtggagtt tcgatgtaac 900

cg 902

<210>785

<211>1026

<212>DNA

<213>Diabrotica virgifera

<400>785

agaacaacgg gagcttcggt tcgcactcaa aatgttatgg cagcctctac tatcgcaaat 60

gttgtaaaga gttcattggg accagttggt ctagataaaa tgttggttga tgacattgga 120

gatgtgacag taactaatga cggagctacc attttacgtc ttttagaagt tgaacatcct 180

gccgccagag tattagtaga attggctcag ttacaggatg aagaagtagg agatggtaca 240

acttcagttg ttatcatagc tgcagaatta ctaaagaatg ctgatgaact cgtgaaacaa 300

aagatacatc caacttcaat tataagtgga tacagattag catgcaaaga ggcttgtagg 360

tacattcagg atcatttgac tattccagtc gaagaattgg gaaaggagtg catcataaat 420

gttgccaaaa cctcgatgag ctcaaaaatt attggagctg atgctgatct tttttcacaa 480

atcgttgtcg atgccgccaa tgctgttaaa gtgacagatt caagaggaaa tagcatgtat 540

cccataaagg ctgtcaatgt cttgaaagca catggtaaaa gcgccagaga gagcttcctt 600

gtaccaggtt atgcattaaa ctgcaccgtt gccagccaag cgatgcccaa aaaaattgtt 660

aacgccaaaa tcgcatgttt agatttttca ttgcagaagg ctaaaatgaa gatgggtgtt 720

caggttttga ttactgatcc aaatcaactg gaagcaatcc gcacaagaga gctcgacatc 780

accaaagaac gcatccagaa aatcctctct accggtgtta atgttattct tgcgagtggt 840

ggtatcgatg atctttgcct aaaatacttc gttgaaaatg gagcgatggg tgtaagaaga 900

gtaaagaaag ctgacttaaa acgcattgcc aaagcaactg gtgccacttt cctaacttct 960

cttagtaaca tggagggaga tgaaagtttt gattctagca tggtaggaga ggcagcggaa 1020

gttgtt 1026

<210>786

<211>469

<212>DNA

<213>Diabrotica virgifera

<400>786

cacattcgca aactctgcct taacatttgt gtaggagaat ctggtgacag gcttactaga 60

gctgctaagg tattggaaca acttacagga caacaacctg tattctcaaa agccagatac 120

actgtcaggt cttttggtat ccgtcgtaat gaaaaaattg ctgtacactg tacagtaagg 180

ggagccaaag ctgaagaaat tttagaaaga ggcttaaaag ttagagaata tgaattgaga 240

cgtgataact tctctgcatc tggaaacttt ggatttggta ttcaggaaca catcgatctt 300

ggtatcaaat atgatccaag tataggtatt tacggtcttg acttttacgt agtattagga 360

agacccggat tcaacgtagc ccacaggagg tcaaagactg gtaaagttgg attctcacac 420

agattgacaa aagaggatgc catgaagtgg ttccaacaga aatacgacg 469

<210>787

<211>1159

<212>DNA

<213>Diabrotica virgifera

<400>787

tatgagcgcc catggacagt atgtatacga atcaagtatg aatgtaggct ttcgcagccg 60

ttttgtaatg gtacattgtt ttctgagcgt ataggtcgtg gtctggaatt atctattgtc 120

atcttcaact gtagtagaca ttatcaaatt tggaatttcg tgtgaatttg tttgttgccg 180

taccaaacaa tccggaaatt tctatacttt cagttggtgt aaagggttat cctaactatc 240

tttatctttg tgctttgtac attttactaa catagattaa tcaataaata aaattaatca 300

ataatactta ttaatttaat gatataatta aaacttttca tggaataaac tttcaagttt 360

gtgaaagcat aaatttggaa ataatattac aaaaaagcac atcatattat actcctttag 420

gataaattag tatattgcca tcatttactt tttacacgtg tttattacat aacgggccca 480

cattgtttta aatgaaaatt tctacaaatt tgtctctaca tttaataatc atctagatca 540

aattcttcgc cgttatccat atcgtctagt tgtaaacttt ggaagtctac tttgtaccgg 600

agcaaacctc caattccacc aaaccctcga acaaattggg agccctcttg agatttgtct 660

gtaataattt ctaaagtggc accgaagttc ttgtaattat tcgccaacca ttcaagaaga 720

ggttgacact cgaccaattc caattctaca ccagtctctt tatccgaaaa atgtgattta 780

tctttttctt gttcaggagt taaatgcagt atagtttccg aacctgtagc atggtttttc 840

aatacatatc gctgaatatc taaattctcc caacagatta gtgtttctac agcacctaat 900

tccaagcccc taagagtatc ttcaactcca aaacagtatt tccccgtatc ttggctaatt 960

tcatcgaaat atctgcctat cagtttcttt tcttgaataa actttacatt ttgaagtgat 1020

tcagctgcta attcgatggc ttgattaaat ccattctcac caccatacga gacatctact 1080

agtttgatta ctttagcttg taaccctgga tcaaacatat cagattgact aagttcagtc 1140

ttaaaatcgg cactacccg 1159

<210>788

<211>1261

<212>DNA

<213>Diabrotica virgifera

<400>788

cctgaagaaa ctcaaagcgc agatgtcgag accttcgcct tccaggctga aatcgctcag 60

ctgatgagtt tgatcatcaa cacattctac tctaataaag aaatcttcct tagagagttg 120

atctcaaact cctcagatgc cttggataag atccgttacc aatcactcac aaatccatcc 180

tgtctcgact ctggaaaaga tttgcacatt aaaattgttc caaacaagag tgaaggaacc 240

ttaaccatca ttgatactgg tattggtatg acgaaagcag atttagttaa caaccttggt 300

acaatcgcaa aatcaggaac aaaggcattc atggaggccc tccaagctgg agctgatatt 360

agtatgattg gtcaatttgg tgtaggtttc tactcagcct acttagttgc tgatcgagta 420

gtggttgtat caaaaaataa cgatgatgaa cagtacattt gggaatcatc tgcaggaggt 480

agcttcacaa taagaccaga tgttggagaa ccattgggac gtggtacaaa aattgttctg 540

tacatcaaag aggatcaaac tgaattttta gaagagaata aaattaagga gattgtaaag 600

aaacactccc agttcattgg ttaccccatt aaattgcttg tagaaaaaga aagggaaaag 660

gaactaagtg atgatgaagc agaagaagaa aagaaggaag aagaggaaga caaagataaa 720

cctaaaattg aagatgtagg agaggatgaa gatgaagata aaaaagatga gaaaaagaaa 780

aagaaaaaaa ctattaaaga aaagtacact gaagatgagg aactaaacaa aaccaaacca 840

atctggacaa gaaatgctga tgatataacc caagaagaat atggtgaatt ttacaaatca 900

ttgacaaatg attgggaaga ccatttagca gtaaaacatt ttagtgttga gggtcaactt 960

gagtttagag ctttattgtt tgttcctcgt cgtgtacctt ttgatttgtt tgaaaacaag 1020

aagaagaaga acaacatcaa attatatgta cgcagagttt tcattatgga caactgtgaa 1080

gaccttatcc cagaatatct taactttatt aagggagtag tagactctga agatttacct 1140

ctaaacattt caagagaaat gttgcaacaa aataagattc ttaaggtcat ccgcaaaaat 1200

ttagttaaaa aatgtctgga actttttgaa gagcttactg aggacaaaga tggatggaag 1260

a 1261

<210>789

<211>905

<212>DNA

<213>Diabrotica virgifera

<400>789

gtgtgaagaa gaagttgccg ctttagtcgt agacaatgga tccggtatgt gcaaagctgg 60

ttttgctggg gatgatgcac ctcgtgctgt attcccttca attgttggac gcccaagaca 120

tcagggtgtg atggtaggaa tgggacaaaa agattcctat gtaggtgatg aagctcaaag 180

taaaagaggt atccttacct taaaataccc catcgagcac ggaatagtca caaactggga 240

tgatatggag aaaatttggc atcatacatt ctacaatgaa ctcagagtag ccccagaaga 300

acaccctgtt ctgttgacag aagctcctct caaccccaag gccaacaggg aaaagatgac 360

acaaataatg tttgaaactt tcaacacccc agccatgtat gttgccatcc aggctgtact 420

ctccttgtat gcatctggtc gtacaactgg tattgtgttg gattctggtg atggtgtatc 480

ccacactgtc ccaatctatg aaggttatgc tcttcctcat gcaatccttc gtttggactt 540

agctggtaga gacttgactg attacctcat gaaaattttg actgaacgtg gctactcttt 600

caccaccaca gcagaaagag aaattgttag agatattaaa gaaaaactct gctatgtagc 660

tttggacttc gaacaagaaa tggcaacagc tgctagttcc agttcccttg aaaagagtta 720

tgaacttcct gatggacaag tcatcaccat tggtaatgaa agattccgtt gcccagaagc 780

tctcttccaa ccatccttct tgggtatgga agctgctggt attcatgaaa caacctacaa 840

ttccatcatg aagtgtgatg ttgatatccg taaggaccta tatgccaaca ctgtactctc 900

tggag 905

<210>790

<211>873

<212>DNA

<213>Diabrotica virgifera

<400>790

cacagacaca aggaaagatg aacaggaacg ttgtattacc atcaaatcaa ctgccatatc 60

tatgttcttc gagttagatg ataaagatct tgtgttcatc accaacgctg accaacgtga 120

gaaagatgtg aaaggtttct tgattaacct tattgactct cctggacacg ttgatttctc 180

atcagaagta acagctgccc ttcgtgtcac tgatggtgcc cttgtagtag tagattgtgt 240

atctggtgta tgtgtacaga ctgaaactgt actccgtcaa gctattgcag aacgtattaa 300

gccaatttta ttcatgaaca agatggaccg tgccctctta gaattacagt tggaatctga 360

agaactttac cagactttcc aacgtattgt tgaaaacgtc aacgtcatta ttgccaccta 420

caacgatgat tcaggtccaa tgggtgaagt cagagttgac cccagcaaag gttcagttgg 480

ttttggatca ggtctccacg gatgggcttt cactctcaaa caattctctg aaatgtatgc 540

cgagaaattc aagattgatg tagttaaact aatgaaccgt ttgtggggtg agaacttctt 600

caaccccaaa accaagaaat ggtccaaaca aaaggatgat gataacaaac gttcattctg 660

tatgtacatc ttagatccaa tctacaagat ttttgactcc atcatgaact acaagaaaga 720

agaatatgaa gcactccttc caaaattggg tattgcctta aaacatgaag ataaagacaa 780

agatggtaaa caattattga aagtagttat gcgtacctgg ttgccagctg gagaagcttt 840

acttcagatg atagccattc acttaccatc acc 873

<210>791

<211>877

<212>DNA

<213>Diabrotica virgifera

<400>791

caaatcaact gctggagaca ctcacttggg aggtgaagac ttcgacaaca gaatggtcaa 60

tcactttgtt ggagaattca agaggaaata caagaaggac cttaccacaa acaaacgtgc 120

cctccgtcgt ttgagaacaa gctgtgaaag agctaagcgt accctttcct catcaaccca 180

agccagcatt gaaatcgatt ctctatttga gggtattgac ttctacacct caatcaccag 240

ggctagattt gaagaactga acgctgattt gttcagatct accatggaac ctgtagaaaa 300

ggccatcagg gatgccaaga tggacaaatc tcaagttcac gatattgtgt tggttggtgg 360

atctacccgt attccaaagg tacaaaaact gttgcaagat ttcttcaatg gtaaagaatt 420

gaacaagtcg atcaaccccg atgaagctgt tgcttatggt gcagccgtcc aagccgccat 480

cttgcacgga gataagtctg aggaagtaca ggacttgctc ttgctcgatg tcactccact 540

ttcattgggt attgaaactg ccggaggtgt tatgactgcc ctcatcaaac gtaacactac 600

cattcccacc aagcaaaccc agaccttcac tacttactct gacaaccaac ccggagtact 660

tattcaggta tatgaaggtg aacgtgccat gaccaaggac aacaacctct tgggaaaatt 720

cgaacttacc ggcattcctc ctgcgccccg tgttgtccct cagattgaag taaccttcga 780

cattgatgcc aacggtatct tgaacgtcac agctattgaa aaatccacca acaaagaaaa 840

caagatcacc atcaccaatg ataaaggtcg tctcagc 877

<210>792

<211>375

<212>DNA

<213>Diabrotica virgifera

<400>792

cgtggattga gaaccgctag gaaacatgtg aatcaccgtc gtgaacaaag atgggctgat 60

aaagattaca aaaaggcaca tttaggaact agatggaagg ctaatccttt tggaggtgct 120

tctcatgcta aaggaattgt attggaaaaa gtgggagttg aagcaaaaca acctaactct 180

gctattcgca agtgcgttag ggtacagtta attaaaaatg gtaaaaaaat cacagccttc 240

gtacctagag atggttgctt aaaccatata gaagaaaacg atgaagttct agttgcaggt 300

tttggacgta aaggtcatgc tgtaggagat attcccggag ttagatttaa ggttgttaag 360

gtcgctaatg tgtcc 375

<210>793

<211>547

<212>DNA

<213>Diabrotica virgifera

<400>793

cgcaactggg tcaccagctt ggattctcat caacatattt tcctttaatt tatatcttgc 60

taacaattct tgtttttctt ctggtgtcat taccacatgt tctggtacca attcatgttc 120

agtaatattg atcaacaact cggattccaa aaattgttct aatatgtatt ttggagccat 180

atcaactagt gactgtttgg cagatggtgt catacctgct tgaacaacaa taatagctct 240

atgaatattt tcttcctgca tacgggaaca ataggtttta atggttttta ttccaatttt 300

tggttcgtct gggaaaaata caaacaactg gtctgtagga tcatcattgt gagctaccaa 360

cacgatcaaa tcacttcttg acggtctttt ctcgctaggt ttgtctccaa actgttcctt 420

aaattgttct aatgtttgat ccaattcatc ttgagttact aggtagcctc tatcgtgaca 480

taactgcatg acagtttttc tgattctcca tagtttgtac gtttcagctt cgtcatccat 540

tatttag 547

<210>794

<211>334

<212>DNA

<213>Diabrotica virgifera

<400>794

gtgtcagatt caccatgatt atcccagtaa ggtgttttac ctgtggaaag gttattggaa 60

acaaatggga ggcttatttg ggtcttctac aagctgaata tacggaaggt gacgctttag 120

atgcccttgg cttgaaacgt tactgctgca gaaggatgct tttgggacat gtggacctca 180

tcgaaaagtt gttgaattat gctcctttag aaaagtgaaa ctgtagcttt aaggtttatg 240

tatataattt aaaaataatg accttatcca tagttaattt gtatatttta gttctacaaa 300

ataatgcatt gactttcata ggcttataat ttgg 334

<210>795

<211>630

<212>DNA

<213>Diabrotica virgifera

<400>795

aagtggtgca ttttgtaacc atcgtttttg tgtattgttt aaagttttca gttgcaaagt 60

gtagatctca taaatataac actctatttt gaaaatggca aacacaaaca cagacttaac 120

ggaagaagac gcagcagacc tacagtttcc aaaagaattt gaaaatgcag aaactttatt 180

aatatcggag gttcacatgt tattagaaca tagaaaggat caaaatgaat ctgcgaagga 240

cgagcaagaa ttttcggatg tatttatgaa aacattaacg tatacagatc gatttaggaa 300

atttaaaaac aaagaaacca tatcagctgt aagaaattta ctaatgcaga ccaaaaagct 360

tcataaattt gagttggctg ctatcgcaaa cttgtgtcca gaaaccacag aggaggcgaa 420

atctctaatc ccttccctgg aagggcgatt tgaagacgag gaacttcagg caatcctgga 480

tgatattcaa actaaaagaa gtatccagta ctagagaaaa aagtcaatag tcatatttaa 540

tttcagattt tttcttttta tattttggaa caatttaatt atttatatta tttgaaaatg 600

caacaacttt ttcacgatgt aatgttagtg 630

<210>796

<211>1152

<212>DNA

<213>Diabrotica virgifera

<400>796

acgccaaacc tcctaagcct aataaaatct ttcctaagac aactacctac aatacgatca 60

tcattgatac ccaagttgac gagaacacaa caataaggcg tratatcaag gaatctcaag 120

catctctctc aaatgagcaa gcattttgat gattctccct ccaggcgtcc aatttaaatg 180

caacatataa aaacaattgt gccgtcaaaa acacgctata aattgccaaa actatataag 240

agaatcaaca aaatcctcga agttgtatgt ttatccctat ttactgtaaa aaagatataa 300

aaaatccaaa attaattatc ttctgtgttt gttagttgat gagacccttg gccttgaaga 360

gatcacgcat gagcccctta aggtacttaa tttcgcgatt tagatcagct atttgacttc 420

ccaaatcaac gttcttgtcc agaagaccct tttcttcact aagaattacc tcaatctcgg 480

ccttcttctt ttggcgatac ctggtagcag cgttcttgtt ttgctccttc ttgcgaacct 540

tcttgtcctc ggtaccgctt ttagagtaag gtctggtcct tttgcgggat gacttaacag 600

gaagttcgtc gttgtactgt tcaattggct ctggcatcca ttccggatca gaagagcagt 660

cgccaaagtt gctgctactg ctactactgc tcggggatcc aggactgctg cttccgctac 720

tgctccattg catgtcttcc acacgagttc tgactagctc gtccacgacg gccagctcca 780

tagcaatatc gggctgagga gtattaacgg gcaaggaata gttgatctcg tagttctctg 840

gcagaaattg cttctccggt tggacgtaaa cacgcttggt ttccactgga tactgtagaa 900

gtggctcgag attggtgagc attggttggc tcgggggaga ctgaggtgga gtgagcgtgc 960

catgggagaa ttccacgact tcgtacacgt tctggaattc cttgaggaaa tattgggtgt 1020

cattggagtt gacaaactca ttcttcaagt tttgaacagg tactggaatg gcacgcggag 1080

gttccacttc gtccaggatt ggtagtattt tttcatcgag aaggaagggt tcgtaacaga 1140

tagacatcat tc 1152

<210>797

<211>988

<212>DNA

<213>Diabrotica virgifera

<400>797

ctcttggatt gatgcgtgag tttagatctt cgattctaac atgtcgacgt tcagcttgta 60

gtaaacgtat gggtaatact ctgattggcc gaatccgagg cctggaatgt caacgctatc 120

aacgttaggt cctgaaaggg cagcactact atcgaggtgg ccgtaaagtt ggttcaagac 180

atctcttaaa cgttttaatt gtttcttatt ggggtggatt aaaatagctt ggaaattgac 240

ggggaggcca tatcttaaaa cagattcaac aaataccctc aacgctttaa cgtgaatcca 300

ggcgcagaaa cactcgctga agttgacttt aagccatcta actaatggac caaattgctt 360

ctttttatcg gtgacgagtt tggttatctc gttctttcca gctgccagtt cctcttcgtt 420

atatatgaaa tcacgaacga tgaacttctt ttctctagcg tgtaacttga attcttcgac 480

gacctttttg aatagggata cagtatatag accgtgttcg ttatcctgtt taatcagttg 540

tgaagaacga gggactatca tgtcggtaat cttttcgtat gtcgcattcc attcgttgaa 600

ttgggctttt ggaactataa ccaacaatgt ctgcaaatat tcagaatcca aaataaaatg 660

ttcctttttc accaaatctg ccaagtttct tgttagtaaa cttcctgtct gtttcttctc 720

caaattctga agacttcctt tcaagttatt gtacgcagac gatttggtct tcagatcagc 780

gtcaatttgg ccaacttgtt tgctgattat atcggcgatg ttacggagcg attgcttgat 840

gggatatttg gcgatgtccc aagtaaatcg ggttaaataa gtgggcaaat cgctgttatt 900

ggcctgcagg ttttctgacc acttgtctct ctgttcttca agtacttctc caaggtactg 960

agctaccttt ctggtgacct gctccacg 988

<210>798

<211>208

<212>DNA

<213>Diabrotica virgifera

<400>798

gagtgcctgg agagctgctg gattaaacta cattaactat tccaacattg ctgccaaact 60

tctgaggcag gcacttaaac ctgaacttag aacagaagca atgagaagaa atgaaaccca 120

tattaaagta accaaatggc aagatggaaa gccaataagg gagtccacat tttaaatcca 180

taatttaagt ccaagtctat gtagtgtt 208

<210>799

<211>1326

<212>DNA

<213>Diabrotica virgifera

<400>799

ctgtatctgg tgtcaacggt ccactagtaa tcctcgaaga tgtcaaattt cctaagttca 60

acgaaattgt ccaactcaga ttatctgacg gaagtttaag atcaggtcaa gtacttgaag 120

tcagcggttc caaggctgta gtacaagtat tcgaaggtac atcaggtatc gatgccaaac 180

acactgtctg tgagttcaca ggagatattc tcagaactcc agtctctgag gatatgttgg 240

gaagagtatt caacggctct ggaaaaccta ttgacaaagg tcccccaatt ctggctgaag 300

actacctaga tattcaagga caacccatca acccatggtc ccgtatctac ccagaggaaa 360

tgatccaaac tggtatcact gccatcgatg tgatgaactc gattgcccgt ggtcagaaga 420

ttcccatttt ctcagctgct ggtctgccac ataacgatat tgctgcccaa atttgtaggc 480

aagccggtct tgtcaaaatc ccaggcaaat cagttttgga tgaccacgaa gacaactttg 540

ccatcgtgtt cgctgctatg ggtgttaaca tggagactgc tagattcttc aaacaagatt 600

tcgaagaaaa tggttctatg gaaaacgtat gtttgttctt gaacttggct aacgatccaa 660

ccatcgaaag aatcatcacc ccacgtcttg ctctaacagc cgctgaattc ttggcttacc 720

agtgcgaaaa acacgtgtta gtcattttaa ctgacatgtc ttcatacgct gaagctttgc 780

gtgaggtatc tgccgccaga gaggaagtac ccggtcgtcg tggtttcccc ggttacatgt 840

acaccgattt ggccaccatc tacgaacgtg ccggtcgtgt cgaaggccgt aacggttcca 900

tcactcaaat ccccatcttg actatgccta acgacgatat tactcaccct attcccgatt 960

tgacaggtta tattaccgaa ggacagatct acgtcgaccg tcaattgcac aacaggcaga 1020

tctacccacc aattaacgta ctgccatctc tctcacgttt gatgaagtct gctatcggcg 1080

aaggtatgac caggaaggac cactctgacg tttctaacca attgtatgct tgttatgcta 1140

tcggtaagga cgtccaggcc atgaaagccg tagtaggtga agaagctcta acacccgacg 1200

atcttctcta cctcgagttc ctaacgaaat tcgaaaagaa cttcatcacc caaggaaact 1260

acgaaaaccg caccgtcttc gagtctttgg acatcggctg gcaactactc cgtatcttcc 1320

ctaagg 1326

<210>800

<211>1034

<212>DNA

<213>Diabrotica virgifera

<400>800

ccgggattta gtaggggcgg tccttccgga aagcgtttct tcacttcctt aatagctttg 60

tgcattcctt ttcgactatc tttcgacctt agatcgttgg gacagaacag ccgcacggac 120

gataaatgag ttatcagcgt cgaatctaca gtcactacct cgatttcgcc tttctggtct 180

tccaaacacg gtttcggtat catcgtttca tcgctatttt ctggtaatac gtgtaacaaa 240

atatccactt tgactttagt ttgggttttg ctttgtcctt gtttattttt gcgccccgga 300

ataatctctt ggaccttttt gaagttaacc acagcgcccc aatcgtattc atcttcagtc 360

gattttatct ttaaaagtct accaggttga agaaacggca acaaatattg cgagcttgtg 420

agatatgtcc taaacttgac acccaacagg tctaactctg ttctaattac gtgataagac 480

tgtattcgct cttcatcttt gatttcaagc gaattgaaaa gttctaattt ttcgttataa 540

tcatcataat atccgggtat tgcagcttga ttctgaaact ggaagaacga tcgctccaac 600

atatactcag gattgatttc ttcgaccctt agcaagttca gtaccatatt gtaagtcaaa 660

tggaaggcgg agttgatggg gtccggcaaa cctttaacta tgttcctacc ggctgccgga 720

ggcactttct cgtccaccat caatattacg atacctttat cgtccaggcc tcttcttcct 780

gcccttccag acatttggat gtattctcca gaagtaatcc atctgtactc actgccatca 840

aatttcctca ttccagtaaa caacacagtt cttgcaggca tatttaaacc catagcgaac 900

gtttctgtgg caaatagcgc ttttatgagc ccctcgccga ataaaatctc gatggtttcc 960

ttcaaaatcg gcaacaaacc accgtgatgg atgcctattc ctcttcttaa caacggcaga 1020

aggttctcga cttg 1034

<210>801

<211>721

<212>DNA

<213>Diabrotica virgifera

<400>801

ggagaaacca tcccctatac aggaagcctc tatccctata gcactgtctg gaaaagatgt 60

acttgctaga gcaaaaaatg gaacaggtaa gactggtgct tacagtattc ctgtgttaga 120

gcaaatcgac cctaaaaaag actgtattca agcactaatt atagtaccaa cgagagaatt 180

agcattacaa acttcacaaa tatgcataga actagccaaa tacctagagg tacgtgttat 240

ggtgacaaca ggtggaacaa acttacgaga tgacattatg cgtatatacc aaaaagtgca 300

agttatcata gcaactccag ggcgtatact tgacttaatg gaaaaaggag tagcagttat 360

ggaccaatgt aaaattcttg tattagatga agctgacaaa ttactgtctc aagattttaa 420

gggaatgctt gacactgtga ttaaaaattt accacaagag aggcaaattt tactattttc 480

tgcaactttc cctttgacag ttgaacaatt tatgagaaag catttacgag atccgtacga 540

gataaatctc atggaagaac ttacattaaa aggtgttaca cagtattatg catttgtcca 600

ggaaagacag aaagtgcact gtttaaatac tctattttcc aagttacaaa ttaatcaaag 660

tattattttt tgcaattcta cacaacgagt agaattgctg gcgaagaaat aacagaactg 720

g 721

<210>802

<211>361

<212>DNA

<213>Diabrotica virgifera

<400>802

gctttagggc agtgtgtaga aactcctcgt ctttacgatc tttcagtaca aggcctttgg 60

cagtcacatt agtaaataga tgcagtgata ttttacaaaa tagctcacaa agtaaacctg 120

gttctttgat cgatatacag acaagattct attcaccaga ggtaataaaa cctaaacctt 180

ctgaagaaga aatcaaacaa agggtgctga aagtgtgttc ttcatatgat aaagtaactg 240

cagaaaagct gactttgaac tcccacttca tcaatgatct tggattagat tccctagacc 300

atgtcgaagt catcatggcg atggaagatg aattcggctt tgaaatccca gatgctgatg 360

c 361

<210>803

<211>183

<212>DNA

<213>Diabrotica virgifera

<400>803

ggtctttcta gcacaattta caattccatt tttaaaagaa cctcaacaat ggctttagct 60

gttgttgttt ctgcattttt cttcgagaga actttcgatt tagcaacaga cacctttttt 120

gaaagatata atgaagggaa attatggaaa cacatcaagg acaaatatga gcaggaataa 180

gtg 183

<210>804

<211>372

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note:

The C4_Dv49_CG8055 concatermer; The combination of inferring,

Carrier activity: CG8055 is directly to homologue

<400>804

cccgggttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 60

gccctcttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 120

gccctcttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 180

gccctcttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 240

gccctcttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 300

gccctcttta ccatggtagc aaagaaaaat gcgtcgaaaa ataaaagagt tgcactccaa 360

gccctctcta ga 372

<210>805

<211>372

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>805

cccgggcaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 60

gctagccaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 120

gctagccaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 180

gctagccaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 240

gctagccaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 300

gctagccaaa tagatggaac ccttacaact attgaaatgc agagggaagc cctcgaagga 360

gctagctcta ga 372

<210>806

<211>490

<212>DNA

＜213〉artificial

<220>

＜223〉artificial sequence note: the C6 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

Be Dv26, Dv49, Dv23, Dv20, Dv13, Dv22, the fragment of Dv18 order is formed

<220>

＜221〉comprehensive characteristics

<222>(1)..(70)

＜223〉concatermer C6 Dv26 fragment

<220>

＜221〉comprehensive characteristics

<222> (71)..(140)

＜223〉concatermer C6 Dv49 fragment

<220>

＜221〉comprehensive characteristics

<222> (141)..(210)

＜223〉concatermer C6 Dv423 fragment

<220>

＜221〉comprehensive characteristics

<222>(211)..(280)

＜223〉concatermer C6 Dv20 fragment

<220>

＜221〉comprehensive characteristics

<222>(281)..(350)

＜223〉concatermer C6 Dv13 fragment

<220>

＜221〉comprehensive characteristics

<222>(351)..(420)

＜223〉concatermer C6 Dv22 fragment

<220>

＜221〉comprehensive characteristics

<222>(421)..(490)

＜223〉concatermer C6 Dv18 fragment

<400>806

catcacgact atcctactag tggagaagta tgcgaagtcg atgtcagaga ttgggaagaa 60

tgcaacaggg ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt 120

tgctactaaa ccaatcaaac gatgaggaac agacagaaca aaccgcaact gaaacggcaa 180

ctacagaggt acagaaaaag ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca 240

cacggtgctg cacaattctt aagagagagg ctgtttgaag tctcttcagc aatagacaat 300

ttgactccct ttcctcttgg gagggaaacg aaggccttag ctcccttacc tcatcgtccg 360

tttggattca caaaaacaca tcgacttctc tctgaaatca cctttgggag gcggtcgtcc 420

gatccttttc gatttgttgc tgccattggg gatccatctg gacttctgga ggaagcaagg 480

caggtgcagc 490

<210>807

<211>560

<212>DNA

＜213〉artificial

<220>

＜223〉be Dv6 by 5 '-3 ', Dv1,

Dv88, Dv93, Dv4, Dv113, the concatermer that the fragment of Dv127 and Dv99 order is formed

<220>

＜221〉comprehensive characteristics

<222>(1)..(70)

＜223〉concatermer C7 Dv6 fragment

<220>

＜221〉comprehensive characteristics

<222>(71)..(140)

＜223〉concatermer C7 Dv1 fragment

<220>

＜221〉comprehensive characteristics

<222>(141)..(210)

＜223〉concatermer C7 Dv88 fragment

<220>

＜221〉comprehensive characteristics

<222>(211)..(280)

＜223〉concatermer C7 Dv93 fragment

<220>

＜221〉comprehensive characteristics

<222>(281)..(350)

＜223〉concatermer C7 Dv4 fragment

<220>

＜221〉comprehensive characteristics

<222>(351)..(420)

＜223〉concatermer C7 Dv113 fragment

<220>

＜221〉comprehensive characteristics

<222>(421)..(490)

＜223〉concatermer C7 Dv127 fragment

<220>

＜221〉comprehensive characteristics

<222>(491)..(550)

＜223〉concatermer C7 Dv99 fragment

<400>807

ggcgcaagcg cttcagtgtt atcatatgcc cacttccagg catttaaatt ccctgactcc 60

atggaagttc tccatcaggg gccaaaaggg gataccaagc cattaccggc cactcatcca 120

cagctttagc atggtacata tagtgatccc aaccgtgcac ctgactgtga cccaactcaa 180

tgtgtaattc cggactgttt ctgctcagcg cccgccacca gttagcgccg aagtacaaaa 240

gggattggac cttatttttg ctggtatcag agctcaacag gcaccacgtg ctcaacgata 300

aaagactggt caggaaggca cattcctttt tgccttactt attcaaggca gggtgcatac 360

agagtagcca cgtaacaaat agcacatagc gaagtgttgt accagcataa tgtataagct 420

cctgtactta ccaaaggctc gttggcagat ctagaccagt tggacgaatt ggttattgag 480

tactgtggtg cagcgccccc tttatggcag atgaatgtct gatggccata ccagacatcg 540

aaggtattga ttacaccacc 560

<210>808

<211>372

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: C8 concatermer; The sodium of inferring/potassium exchange

ATP enzyme: CG9261 is directly to homologue

<400>808

cccggggcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 60

ggagaagcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 120

ggagaagcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 180

ggagaagcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 240

ggagaagcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 300

ggagaagcta aaggaaatgg ccaaattaaa acatgctctc atcacgacta tcctactagt 360

ggagaatcta ga 372

<210>809

<211>372

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: C9 concatermer; The sodium of inferring/potassium exchange

ATP enzyme: CG9261 is directly to homologue

<400>809

cccgggctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 60

acgatgctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 120

acgatgctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 180

acgatgctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 240

acgatgctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 300

acgatgctcc atgtattttt attaaattga acaaaatata taactgggag ccaatgtatt 360

acgatgtcta ga 372

<210>810

<211>1000

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: C10 concatermer with gene of the identical/different binding modes of inferring

About 50%GC standard

<400>810

caagcccgcc aaaatgcgcg aacatctgga actcttctgg tctcgagtga atatccccaa 60

agttcttagg aatgcaatgc ttcctagacg tcgtcgagct ggctctgaaa ctgagcccca 120

agttatagta ggtaacgata attattggac gtggcattag gttataggga aagtgtcaat 180

ctgtctcaaa ttcgcacatt tgtggaaatg gactcgcacg cgcggcccaa cgacggcttc 240

aacaaacaca ggcgcaagtc gacgaagtcg tggatatcat gaaaacgaat gtagaaaaag 300

gtatggcagg aaagaaatgc tgaatccgcc attgaagctc ttaaagaata tgaaccagaa 360

atgggcaaag ttgtccgaca agacaaatcc ggtattcaga aaatccgggc atagcaaaca 420

tgccaaacac acacacgcag aaaggaaata tcagtcagac tcagaatcag atagtgatct 480

ggaatggatt ggacacagag gtgaatcaga ccagaggacg ttcgggggta tattaaaaat 540

aacgttgtgt gcgcacttgg aaccgaaaca agcagaccat catccgcagc tcagtgcgta 600

gccgaatgtg gccgtgcaca ctgtgcattt gtaggacagg tgtgcgggtt tcaacgtcat 660

gttatgatcc cttgcatata ttgatggaaa aacacgccaa agaagagagc agagtattaa 720

aaggagtatt gccaggtaca tagaccagca aggaactgta atcaaaatgg gcgatgaaaa 780

gaaggccagc gatagcgaac acattaatct caaagtactt ggtcaggata atgcagtggt 840

gcagaaggtc agacctctcc tcaacgtcac ccgtatcgag gatgagattg ccaaacttga 900

agagaaagcc caaaaagccc aacgaacacg gcgtagacga aacatcttgt gtagttattg 960

tagaaggaca aaccagcaca ccagaacaac aagaactagc 1000

<210>811

<211>478

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note:

The C12 concatermer of highly effective WCR target, about 50%GC standard,

Form for the fragment of Dv23-Dv13-Dv26-Dv18-Dv49-Dv22-Dv20 order by 5 '-3 '

<220>

＜221〉comprehensive characteristics

<222>(1)..(70)

＜223〉concatermer C12 Dv24 fragment

<220>

＜221〉comprehensive characteristics

<222>(71)..(145)

＜223〉concatermer C12 Dv13 fragment

<220>

＜221〉comprehensive characteristics

<222>(146)..(222)

＜223〉concatermer C12 Dv26 fragment

<220>

＜221〉comprehensive characteristics

<222>(223)..(275)

＜223〉concatermer C12 Dv18 fragment

<220>

＜221〉comprehensive characteristics

<222>(276)..(338)

＜223〉concatermer C12 Dv49 fragment

<220>

＜221〉comprehensive characteristics

<222>(339)..(418)

＜223〉concatermer C12 Dv22 fragment

<220>

＜221〉comprehensive characteristics

<222>(419)..(478)

＜223〉concatermer C12 Dv20 fragment

<400>811

cctcaagctg tcattggcat ggatattctg tgccaagcta aatccggtat gggaaaaacg 60

gctgtttttg caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc 120

aaatttcttc ctccagtgat catacccata cgatttacct gaggaaatgc cggataatct 180

gaagcaacat ataaggggta tcaacaatac tagagagttg agtcaaacat aattatggca 240

cattgtcctt ggatgtagta tccatcacgc agaccgccga acaacacgac atagccaacg 300

aaatcacaaa cgctattagc aatcctgtcg gattcaccgt aatgcactcc tacgtcgttt 360

ggttcgtatt ggagtactag acgagaacag aatgaagctc gattacgtat tgggtctccg 420

aagtaatgta caacggccac actggacgta agataaatgc tcagattttc ttaggacc 478

<210>812

<211>869

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: artificial sequence note:

The C14 concatermer of activated gene target in some different biologies, about 50%GC standard

<400>812

ccgaagtgag atcggagcca aaaagcattg gttgcttgac ggtccaatgg aaaacgaacg 60

agaagtggat ggcttcagca atgctcggcc aggctgattg ctctttattt tgacactgga 120

atgtacagcg aagcgctaca actggaatcg accttactga ctactcgtct acgatatcgc 180

caaacatctg acatacgaaa acgttgaacg ttggttgcgc gaattgcgcc attcttttct 240

caggtaccaa tgttgctgct ggtaaagcac gtggtattgt tattggaact ggtcttaaca 300

ccgccattgg taaaatcaga actgaaatgt ccaacgaaat tgccaacact aactcaagac 360

agaacatccg taagttgata aaggatggtc ttattattaa gaagcccgta gctgtacatt 420

cccgtgccga tacctacaca gaaagctata ttagtaccat tggcgtagat tttaaaatca 480

ggacaatcga tttagatggg ttacaaaagg ataccaatac aagatgagag cagcatatgc 540

tcactttccc attaactgtg tcaccactga aggaaacaca gttatccagt ttgcaagctc 600

tctggcagat ttcagtcaaa tcggcaccag aaaatccatg agttacttta gcaatgtata 660

caagatctac gtctttagct acaggcgatt tccgatgaaa tagtggcatt aggttatagg 720

gaaagtgtca atctgtctca aattcgcaca tttgtggaaa tggactcgca cgaagaaaaa 780

gtttatcagg ccgtgagaga attaaaagtt caaggaggta tcggttcgtg tgtatcactt 840

aacgtgaaga gccccttggt ttccgacac 869

<210>813

<211>2694

<212>DNA

<213>Diabrotica virgifera

<400>813

ggtcgaccca cgcgtccggc ttgagtaaaa actgaaatca tataaaagag ataatttcta 60

ttgtgaacta atttttcaaa agtttctaca ttaagtacat attcattact aaataatggc 120

cgaaaacaaa aatccagagg atttggccac cgcaattctc cgtaagaaag atcgtccaaa 180

tagacttttg gtagaagaag cagttaacga tgataactcc gtggttgctt tgtcacaagc 240

aaaaatggat gaactacaac ttttcagagg agacacagtc ctactgaagg gcaagcgaag 300

gaaagaaact gtttgtatag tattatctga tgatacatgt agcgatgaaa aaattcgtat 360

gaatagaaat aatttaagag ttaggctttc agatgtagtt tctatccaag cttgccctga 420

tgtaaagtat ggcaagagaa tacatgtact tcccatagat gatacagtgg aaggactcac 480

aggaaatctc ttcgaagtat acctgaaacc ctacttcctg gaggcctatc gaccaataca 540

caaagatgac gtattcgttg tacgtggagg aatgagagct gttgaattca aagtcgtcga 600

gaccgatccc gctccgtact gtatcgtggc tccagacacc gtcattcatt gtgatggaga 660

tccaatcaag cgcgaagagg aagaggaagc tcttaacgca gttggatacg acgacatcgg 720

aggttgtaga aaacagcttg ctcaaatcaa agaaatggta gagttaccac tgagacatcc 780

tagtcttttc aaggctattg gagttaaacc accaagaggt attttacttt acggtccacc 840

gggtactggt aaaacactca ttgcacgtgc agtagctaac gaaactggag cgttcttctt 900

cttgattaac ggtcctgaga ttatgagcaa attggccggt gaatccgaaa gcaatcttcg 960

taaagctttc gaggaagccg acaagaattc cccagctatt attttcattg atgaattgga 1020

tgccatcgct cccaagagag agaaaacaca cggagaggtg gaacgccgta tagtatccca 1080

acttctaact ttgatggacg gtatgaagaa gagttcccac gttatcgtta tggccgctac 1140

taacagacca aactcaatcg atccagcttt acgtagattc ggaagattcg acagggaaat 1200

cgatatcgga atacctgatg caactggcag acttgaagtt ttacgtattc acaccaagaa 1260

catgaaattg gccgatgatg tcgacttgga acagatcgct gcggaaactc atggtcacgt 1320

aggtgccgat ttagcttcac tatgttcgga agcagccctt caacaaatca gagaaaaaat 1380

ggacctcatc gacctcgaag acgaccaaat tgacgcagaa gtcctaaact cactggcagt 1440

taccatggag aacttccgtt acgccatgac gaagagcagc ccaagtgctt tacgtgagac 1500

catagtagaa gtaccaaacg tcacttggga cgatatcggt ggcttggcta atgtcaagaa 1560

agagttgcaa gaattggtgc aatatccagt cgaacatcca gataagttcc ttaagttcgg 1620

tatgcaaccg tccagaggag tgttgttcta tgggccgcct ggttgcggta aaacgttatt 1680

agctaaggct attgcgaatg aatgccaagc caactttatc tcagttaaag gaccagagtt 1740

gcttactatg tggtttggtg aatcagaagc aaatgtccga gatatcttcg acaaggctcg 1800

atctgcggca ccttgtgttc tattctttga cgaattagat tcaattgcca aatcgcgtgg 1860

tggaaatgta ggtgatgccg gtggtgcagc cgacagagtt attaatcaaa ttctaacaga 1920

aatggacggt atgggagcca aaaagaacgt gtttattatc ggtgctacaa acagaccaga 1980

tatcatcgat cctgcaatat tgcgtccagg tcgtctcgat cagttgatct acatccctct 2040

gcccgacgag aaatctaggg aagccatctt caaagccaac ttgaggaaat cgcctgtagc 2100

taaagacgta gatcttgtat acatcgctaa agtaacccat ggattttctg gtgccgattt 2160

gactgaaatc tgccagagag cttgcaaact tgccatcagg caaagcatcg aaacagaaat 2220

taggagagaa agagaaaggg ctttgaaccc caatgttgct atggatttgg acgatgacga 2280

tccagtccca gaaatcacca gggcccmctt cgaagaggcc atgcgtttcg ccagacgttc 2340

cgtttcagac aacgacatca gaaaatacga gatgttcgcc cagaccttac agcaatctcg 2400

cggattcggt accaacttcc gtttcccgac agcagctggt ggacctgctg ctccaggagg 2460

taccggagga gaccaggcca acttccagga cgatcctgaa gacgatctgt atagttaaat 2520

gtttcaggtg ggctcaataa gcaccaactt tcaattttat ttttcatttt tgtatttatt 2580

tacagtaact cctcagtttg ctaacaatat tacattgtta acgcattcat atgttgttta 2640

atataatagt tttggaatat aattacaagt ttgtctaaaa aaaaaaaaaa aggg 2694

<210>814

<211>622

<212>DNA

<213>Diabrotica virgifera

<400>814

gtcacaagta gggctaaaat gaagcaaatt gtaacaaacc aaacggtaaa aattcctgag 60

ggaattacag taaccactaa gtcaagggta gtaactgtaa aaggaccccg aggtaatctg 120

aagagatcct tcaaacatct taccctggac attcgaatga tcaaccccag gttactgaaa 180

gtagagaaat ggttcggtac caagaaggaa ttagctgctg tcagaacagt atgctctcat 240

gttgaaaaca tgttaaaggg tgttacaaaa ggataccaat acaagatgag agcagcatat 300

gctcactttc ccattaactg tgtcaccact gaaggaaaca cagttatcga aattagaaat 360

ttcttgggag aaaaatacat cagaagagta aagatggccc caggtgttac agtagtaaac 420

tccaccaaac aaaaggatga acttatcatt gaaggaaact cattggagga tgtttcaaag 480

tcagctgccc ttattcaaca atctacaaca gttaaaaata aggatatccg taaattctta 540

gatgggcttt atgtatctga aaaaacaact gttgtacaag aagaataact tgtatttttg 600

aaataaattt aagtttatat tt 622

<210>815

<211>670

<212>DNA

<213>Diabrotica virgifera

<400>815

aaattctttt acggaagaca cgttgagagg tgcatttgca cgatgagttc cttaaaactt 60

cagaagaggc tagcagcctc tgttatgcga tgtggtaaaa agaaagtatg gttggaccct 120

aatgaaatca acgaaattgc caacactaac tcaagacaga acatccgtaa gttgataaag 180

gatggtctta ttattaagaa gcccgtagct gtacattccc gtgcccgtgt tcgcaaaaac 240

actgaagccc gcaggaaagg aaggcactgc ggttttggta aaaggaaggg tactgctaat 300

gcccgtaccc crcaaaagga attatggatt caacgcatga gagttttgcg tcgtctcctt 360

aaaaaataca gggaagctaa aaaaattgac agacatctat accactcact ctacatgaag 420

gccaagggta acgtattcaa gaacaagcgt gtccttatgg aatacatcca caagaagaag 480

gcagagaaag cccgtgccaa gatgttggca gaccaggcca atgccagaag gatgaaggta 540

aaacaggcta gagaaagacg tgaggaacgt atcgccacaa agaaacaaga agttttgcag 600

aactacatga gggaggatga agctgcggcc actaagaaat aagttaattg ttttataaga 660

tgactatatt 670

<210>816

<211>871

<212>DNA

<213>Diabrotica virgifera

<400>816

gttcgcctga tctgtctttc atatttttaa agtcaagatg gctcgaggac ctaaaaagca 60

tttgaagcgt ttaaacgcac ccaaagcatg gatgttggac aaattgggag gtgtatttgc 120

cccccgtcca tccaccggtc ctcacaagtt gagagaatct ttaccattag taattttctt 180

aagaaacaga ctaaagtatg ccctcaccaa cagtgaagtc acaaaaattg ttatgcaaag 240

attaattaaa gtcgatggaa aagtcagaac agacagtaac tatcctgcag gatttatgga 300

tgttattact attgagaaga ctggtgaatt cttccgtctt atctatgatg ttaaaggtag 360

atttgccatt caccgtatta cagctgaaga agcaaaatac aaactctgca aagtacgaag 420

agtccaaact ggaccaaaag gaattccatt cttggtgaca catgatggcc gtaccatcag 480

ataccctgac ccaaacatca aagtcaatga caccatccaa ttggaaattg ccacatctaa 540

aattttagac tacattaagt tcgaatctgg aaacttatgt atgatcactg gaggaagaaa 600

tttgggtcgt gtaggtacag ttatcaatag ggaacgtcat cctggatcct ttgatattgt 660

gcatgttaaa gatgctaatg gtcatgtatt tgctactagg ttaaataacg ttttcattat 720

tggtaaggga gctaaggcct tcgtttccct cccaagagga aagggagtca aattgtctat 780

tgctgaagag agagacaaga ggttagcagc caaaacacat taaatgtaat ggttgattac 840

attattttca ataaaactta aaaaatctga a 871

<210>817

<211>2410

<212>DNA

<213>Diabrot ica virgifera

<400>817

cggacgcgtg ggggagaaac ataacatcca tccacaaata tgtcgaaagt aaggatcgga 60

gatgaagaga aggaagggca gtatggttat gtccatgctg tctcaggtcc agtcgttact 120

gctgagaaaa tgtctggttc tgctatgtac gaactggtac gtgtcggata ctatgagctg 180

gtaggagaaa tcattagatt ggaaggtgac atggctacta ttcaggtata cgaagaaaca 240

tcaggtgtaa ctgttggtga tccagtatta agaactggta aaccactttc agtagaactt 300

ggacctggta ttatgggttc catttttgat ggtatccaac gtccattgaa agacatttgt 360

gacgctactg atagtattta catccccaag ggtattaacg taccttcttt atcgagaaca 420

gcaaaatggg acttcaaccc aatcaacatc aagttgggat ctcacttaac tggaggtgat 480

atatatggtc tagttcatga aaacaccctt gtcaaacaca aaatgattct gcctcctaga 540

gctaagggta ctgtaaccta cattgcagaa ccaggaaact acactgttga tgaagtagta 600

ttggaaactg aatttgatgg tgatcgtacc aaatatacta tgttgcaagt atggcctgta 660

cgtcaagcaa ggccagtcag tgaaaaatta cctgccaacc atcctctgct tacaggacag 720

cgtgtacttg atgctctttt cccatgtgta cagggtggta ctactgccat tcccggagct 780

ttcggttgtg gaaaaactgt aatttcacaa tctctttcca aatattccaa ctctgatgtc 840

attatctacg tcggttgcgg agaaagaggt aacgaaatgt ctgaagtatt gagagatttc 900

cctgaattga ctgttgaaat tgacgggcac actgaatcta ttatgaaacg taccgcattg 960

gtcgccaaca catctaacat gcctgtagct gctcgtgaag cttctatcta tactggtatt 1020

actctttctg aatacttccg tgatatgggt tacaacgtat ctatgatggc tgactcgaca 1080

tcacgttggg ccgaagcttt gagagaaatt tcaggtcgtt tggctgaaat gcctgccgat 1140

tccggttatc cggcttactt aggtgcccgt ttggcttcct tctacgaacg tgctggtcgc 1200

gttaaatgtt taggtaatcc agacagagaa ggatccgttt caattgtagg agccgtatca 1260

cctcctggtg gtgatttctc agatcctgtt accactgcta ctcttggtat tgtacaggtg 1320

ttctggggtt tggacaagaa acttgcccaa cgtaagcact tcccttcagt agactggctt 1380

ggatcatatt ccaaatattt aagagcattg gacgactttt atgacaaaaa cttccaagag 1440

tttattcctc ttagaaccaa agttaaggaa attcttcagg aagaagatga tctagccgaa 1500

attgtgcagc tggtaggtaa agcatctctg gcagaaacgg acaaaatcac cttggaaatt 1560

gccaggcttc ttaaagaaga tttcttgcaa caaaactcat actcttctta tgacagattc 1620

tgtccattct ataaaactgt cggtatgttg agaaacatga tcggtttgta cgacatggcg 1680

agacacgctg tagaatcaac cgcacaatca gaaaataaga tcacttggaa cgtaataaga 1740

gattcaatga gtggaatttt atatcaactt agcagtatga aatttaagga tcccgtaaaa 1800

gatggtgaag ctaaaatcaa ggcagatttt gatcaattat atgaagatat tcagcaggcc 1860

ttcagaaact tagaagatta aatcttttta aggaaatttt cctattttgt tcatcagtgt 1920

aagtttaaaa atatagcgat atttatcaaa aagaataata aggcctctat ccctcacttc 1980

tgtgaatatt aatatggccg tactaaagat agtaactaaa gataggtttt ctcttttttg 2040

atattatcct gtacaaaata aattatgtaa attgttgaat atgtgtatag tttttttggg 2100

tgagggtaca gtgcttatta aatacttttt aaacattttt cccgccattc caattactat 2160

taagtttttt cgttttaata cttttttaaa tatacaggtg cttaatatcg tttatatttt 2220

cagtattact tggttttctt catgtaaatt gttttaaatt tttcttttac ccttttaatc 2280

ttgtatatta cattacccaa ttaaagttaa ttgtacagat taagataaac gagtatctta 2340

taacatctat tagattgtta gaatcaataa atgtagtgta attgttctgt tttgaacaaa 2400

taaatgcatc 2410

<210>818

<211>988

<212>DNA

<213>Diabrotica virgifera

<220>

＜221〉base of Xiu Shiing

<222>(884)..(965)

＜223〉n=a, c, g or t/u

<400>818

aggacgcgtg ggttcgtatt tttttcttaa caaaagggca aaatgagctt ttttggaaaa 60

ttgttcgggg ggaaaaagga agagatagcc cctagtcctg gggaggctat tcaaaaactc 120

agagagactg aagaaatgtt aataaaaaaa caggattttt tagaaaagaa gatagaagaa 180

tttaccatgg tagcaaagaa aaatgcgtcg aaaaataaaa gagttgcact ccaagccctc 240

aaaaagaaga aacgattgga aaagacccaa ctacaaatag atggaaccct tacaactatt 300

gaaatgcaga gggaagccct cgaaggagct agcacaaata ctgctgtatt agattctatg 360

aaaaatgctg cagatgccct taagaaagct cataagaatt tgaatgtaga tgatgttcac 420

gatatcatgg atgacatagc cgaacaacac gacatagcca acgaaatcac aaacgctatt 480

agcaatcctg tcggattcac cgacgatctg gatgacgatg aattagaaaa agaattagaa 540

gagctcgaac aagaaggatt ggaagaagac ctgctccaag tgccaggtcc aactcaactg 600

ccggctgtgc ctgctgatgc agttgctact aaaccaatca aaccagcagc taaaaaagtt 660

gaagatgatg acgatatgaa agaattggaa gcctgggcct cgtaaaattc ctgaaatctg 720

gatatttgta aacgaaatca cccatctaag atgaaaacat tataaatata taggtaataa 780

cagctaaaaa cgtttcaatg tagaacaagc ttttgctgaa aaggcgtctt gcaaaaaatg 840

ttgataattt agaattctct atatattata tatttgccct ttangaaaga tttcttttat 900

agtcatagtt caaccanacg tttcataaat agaattacag gggtgtcaga attctacngt 960

gattnattga aaaaaaaaga cttttcct 988

<210>819

<211>3001

<212>DNA

<213>Diabrotica virgifera

<400>819

aagcagtggt aacaacgcag agtacttttt tttttttttt ttttttttga cagttcgaaa 60

atggtgatgt gtttataatt cgtgtcaatt tagtgaaata gcattcaatt ttttgtataa 120

gatctcttca aaatgccact tcgattagat ataaaaagaa agctaacagc tcgctcagac 180

cgggtaaaat gtgtggatct tcaccctaca gaaccttgga tgctgtgttc tctttacagc 240

ggaaatataa acgtttggaa caccgaaaat cagcaactgg ttaagacttt tgaagtatgt 300

gatgtacctg ttcggacagc taagtttttg cccaggaaga actggatagt cagtgggtct 360

gatgatatgc agattcgagt tttcaattac aataccttag atcgggtaca ttcttttgag 420

gctcattcgg attatgtgag atgtattgtc gtacacccta cacaacctta tatattaaca 480

agtagtgatg atatgcttat caagctttgg aattgggaaa aagcatgggc ttgtcagcaa 540

gttttcgaag gacacactca ttatattatg caaatcgcca taaatccaaa agacaacaac 600

acatttgcca gtgcatccct agatagaaca ttgaaagtat ggcaattggg agcgtccaca 660

gcgaatttca cactagaagg tcatgagaaa ggcgttaact gtgtggacta ttatcacggt 720

ggagataaac cttatttaat ctcaggcgct gatgatagat tagtaaaaat ctgggattat 780

caaaacaaaa cttgtgttca aactttggaa ggacatgctc aaaatgtaac cgctgcatgt 840

ttccatccag aacttcctgt agctcttact ggaagtgaag atggtactgt cagagtgtgg 900

catgccaaca cccataggtt agaaagtagc ttaaattatg gctttgaaag agtatggact 960

attttctgcc taaagggatc caataacgtg gcattgggtt atgatgaagg tagcattttg 1020

gttaaagttg gtagagaaga accagctgtt agtatggatg ccagtggagg caaaattatt 1080

tgggccagac actctgaact tcaacaggca aatctcaagg cgttagctga aggtgcggaa 1140

ataagagatg gagaacgcct tccagtttct gtaaaagata tgggtgcttg cgagatatac 1200

cctcagacaa ttcaacacaa tcccaatggc cgttttgttg ttgtctgtgg ggatggagaa 1260

tacataatct acacagcaat ggctttaaga aacaaagcgt ttggtagcgc acaagaattt 1320

gtgtgggctc aagattccag cgaatatgcc atcagagaat ccggatctac tatcagaatt 1380

tttaagaatt tcaaagagaa gaagaatttt aagtccgatt ttggagctga aggtatatac 1440

ggtggatacc ttttgggagt caaatcggtt tctggtttga ctttctatga ttgggaaact 1500

ctcgatttag tcagaagaat cgagatacaa ccaaaagcag tttactggtc agatagtggt 1560

aaattagtat gtttggccac agaagatagc tactttattc tttcttatga ttctgatgaa 1620

gttcaaaaag ccagagataa caatcaggtt gcggatgatg gagtagaatc ggctttcaat 1680

cttctaggtg aaataaacga atcagtgcga actggtctct gggtaggcga ctgttttatc 1740

tacacgaatt ctgttaatcg tatcaactac ttcgttggag gtgaactggt tacaattgct 1800

catttggacc ggcctttgta tgtcttggga tatgtgccta aagacgatag attatacctc 1860

gtagataaag agttgcgcgt agtaagctac caattacttc tttctgttct tgaatatcaa 1920

actgccgtca tgagaagaga ctttccaaca gcagacagag tacttccgtc cattcctaag 1980

gagcacagaa cgagagtggc acatttctta gaaaagcaag gcttcaaaca gcaagctttg 2040

gccgtaagta cagatccaga gcacagattc gagctggcag tagcattaga ggatcttaat 2100

atagccaaaa ctctagctca agaagcgaac agtccgcaaa agtggaatca actagcagaa 2160

ttggcagctg ctactaataa tgtaagcgta gccaaggaat gtatgcaaaa agcgcaagat 2220

tatggaggct tgttgcttct tgctacgagc tccggtgatg aaaatttagt ccgtactcta 2280

ggagaaacga cacaagctga aagcaaacat aacttagcat ttttgtcaca cttgttagta 2340

ggtgatttaa acaaatgtct agacattctt attaataccg gtagattgcc agaagctgca 2400

tttttcgcca gatcttacct tcctgataag attacagaag tcgtggaact gtggaagact 2460

cagttatctt cagtcaatca aaaagctgga cagagccttg ccgatcctaa aaactacgaa 2520

aatctgttcc ctggtttaca agaggcggtg gtagctcaga aatttttgga acagcagaat 2580

aaaggtttag cgcccgcaag agttgccacc accattcctc ctaatcacga caggaatgtt 2640

gtagccgaag ttcaagcaca atcgaaaccc gatgtaccat catttagttc ttcgtttatt 2700

tcatcagaaa tagaagcaca aacaaggagt tttgctaaac ctgaagaatt ttcaaacatt 2760

atacagctgg accaagatga cgacgatatc gatttagatt tggacggtgt aaatatcgat 2820

gagaacattg acccgacgga tatcaacatc gatgatgatt tgctgagtga ttgaaaataa 2880

cttttttact ttagtattaa atctgtatat tcattcctat tcttaagaaa atctatatga 2940

attttaatgt tttaatagtt aagaaatcca aaatttaaaa aaaaaaaaaa aaaaaaaaaa 3000

a 3001

<210>820

<211>2719

<212>DNA

<213>Diabrotica virgifera

<400>820

ggtccgattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc 60

ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc 120

catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa 180

gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca 240

aagcaagtgg attgatgtga tggtccgatt gagacttttc aacaaagggt aatatccgga 300

aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag 360

gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc 420

tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 480

gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 540

gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 600

catttggaga ggacacgctg agggcccacc gtcttcggta cgcgctcact ccgccctctg 660

cctttgttac tgccacgttt ctctgaatgc tctcttgtgt ggtgattgct gagagtggtt 720

tagctggatc tagaattaca ctctgaaatc gtgttctgcc tgtgctgatt acttgccgtc 780

ctttgtagca gcaaaatata gggacatggt agtacgaaac gaagatagaa cctacacagc 840

aatacgagaa atgtgtaatt tggtgcttag cggtatttat ttaagcacat gttggtgtta 900

tagggcactt ggattcagaa gtttgctgtt aatttaggca caggcttcat actacatggg 960

tcaatagtat agggattcat attataggcg atactataat aatttgttcg tctgcagagc 1020

ttattatttg ccaaaattag atattcctat tctgtttttg tttgtgtgct gttaaattgt 1080

taacgcctga aggaataaat ataaatgacg aaattttgat gtttatctct gctcctttat 1140

tgtgaccata agtcaagatc agatgcactt gttttaaata ttgttgtctg aagaaataag 1200

tactgacagt attttgatgc attgatctgc ttgtttgttg taacaaaatt taaaaataaa 1260

gagtttcctt tttgttgctc tccttacctc ctgatggtat ctagtatcta ccaactgaca 1320

ctatattgct tctctttaca tacgtatctt gctcgatgcc ttctccctag tgttgaccag 1380

tgttactcac atagtctttg ctcatttcat tgtaatgcag ataccaagcg ggagctcgac 1440

gtccctcagc agtcgctgtg cgatacccat cgtcatccag atcgtcggtg aatccgacag 1500

gattgctaat agcgtttgtg atttcgttgg ctatgtcgtg ttgttcggct atgtcatcca 1560

tgatatcgtg aacatcatct acattcaaat tcttatgagc tttcttaagg gcatctgcag 1620

catttttcat agaatctaat acagcagtat ttgtgctagc tccttcgagg gcttccctct 1680

gcatttcaat agttgtaagg gttccatcta tttgtagttg ggtcttttcc aatcgtttct 1740

tctttttgag ggcttggagt gcaactcttt tatttttcga cgcatttttc tttgctacca 1800

tggtaaattc ttctatcttc ttttctaaaa aatcctgttt ttttattaac atttcttcag 1860

tctctctgag tttttgaata gcctccccag gacaagtact gcgatcgcgt taacgcttta 1920

tcacgatacc ttctaccaca tatcactaac aacatcaaca ctcatcactc tcgacgacat 1980

ccactcgatc actactctca cacgaccgat taactcctca tccacgcggc cgcctgcagg 2040

agcgtcctgg ggaggctatt caaaaactca gagagactga agaaatgtta ataaaaaaac 2100

aggatttttt agaaaagaag atagaagaat ttaccatggt agcaaagaaa aatgcgtcga 2160

aaaataaaag agttgcactc caagccctca aaaagaagaa acgattggaa aagacccaac 2220

tacaaataga tggaaccctt acaactattg aaatgcagag ggaagccctc gaaggagcta 2280

gcacaaatac tgctgtatta gattctatga aaaatgctgc agatgccctt aagaaagctc 2340

ataagaattt gaatgtagat gatgttcacg atatcatgga tgacatagcc gaacaacacg 2400

acatagccaa cgaaatcaca aacgctatta gcaatcctgt cggattcacc gacgatctgg 2460

atgacgatgt agatcgccag cggtactcgc tgaggcctag gctgcatgcg tttggacgta 2520

tgctcattca ggttggagcc aatttggttg atgtgtgtgc gagttcttgc gagtctgatg 2580

agacatctct gtattgtgtt tctttcccca gtgttttctg tacttgtgta atcggctaat 2640

cgccaacaga ttcggcgatg aataaatgag aaataaattg ttctgatttt gagtgcaaaa 2700

aaaaaggaat taagcttac 2719

<210>821

<211>2367

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: pM0N98504

Dv49 concatermer C1 dsRNA expression cassette

<400>821

ggtccgattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc 60

ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc 120

catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa 180

gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca 240

aagcaagtgg attgatgtga tggtccgatt gagacttttc aacaaagggt aatatccgga 300

aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag 360

gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc 420

tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 480

gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 540

gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 600

catttggaga ggacacgctg agggcccacc gtcttcggta cgcgctcact ccgccctctg 660

cctttgttac tgccacgttt ctctgaatgc tctcttgtgt ggtgattgct gagagtggtt 720

tagctggatc tagaattaca ctctgaaatc gtgttctgcc tgtgctgatt acttgccgtc 780

ctttgtagca gcaaaatata gggacatggt agtacgaaac gaagatagaa cctacacagc 840

aatacgagaa atgtgtaatt tggtgcttag cggtatttat ttaagcacat gttggtgtta 900

tagggcactt ggattcagaa gtttgctgtt aatttaggca caggcttcat actacatggg 960

tcaatagtat agggattcat attataggcg atactataat aatttgttcg tctgcagagc 1020

ttattatttg ccaaaattag atattcctat tctgtttttg tttgtgtgct gttaaattgt 1080

taacgcctga aggaataaat ataaatgacg aaattttgat gtttatctct gctcctttat 1140

tgtgaccata agtcaagatc agatgcactt gttttaaata ttgttgtctg aagaaataag 1200

tactgacagt attttgatgc attgatctgc ttgtttgttg taacaaaatt taaaaataaa 1260

gagtttcctt tttgttgctc tccttacctc ctgatggtat ctagtatcta ccaactgaca 1320

ctatattgct tctctttaca tacgtatctt gctcgatgcc ttctccctag tgttgaccag 1380

tgttactcac atagtctttg ctcatttcat tgtaatgcag ataccaagcg ggagctcgac 1440

gtccctcagc agtcgctgtg cgataccata tcttcatata attgatcaaa atctgccttg 1500

attttagctt caccatcgtg attttgtccg tttctgccag agatgcttta cctaccagct 1560

gcacaatatt acctaaacat ttaacgcgac cagcacgttc gtagaaggaa gccaaacgcg 1620

accaatgcgg tacgtttcat aatagattca gtgtgcccgt caatttcggt tggcaggtaa 1680

tttttcactg actggccttg cttgacgtac aggccataag tactgcgatc gcgttaacgc 1740

tttatcacga taccttctac cacatatcac taacaacatc aacactcatc actctcgacg 1800

acatccactc gatcactact ctcacacgac cgattaactc ctcatccacg cggccgcctg 1860

caggagcatg gcctgtacgt caagcaaggc cagtcagtga aaaattacct gccaaccgaa 1920

attgacgggc acactgaatc tattatgaaa cgtaccgcat tggtcgcgtt tggcttcctt 1980

ctacgaacgt gctggtcgcg ttaaatgttt aggtaatatt gtgcagctgg taggtaaagc 2040

atctctggca gaaacggaca aaatcacgat ggtgaagcta aaatcaaggc agattttgat 2100

caattatatg aagatattag atcgccagcg gtactcgctg aggcctaggc tgcatgcgtt 2160

tggacgtatg ctcattcagg ttggagccaa tttggttgat gtgtgtgcga gttcttgcga 2220

gtctgatgag acatctctgt attgtgtttc tttccccagt gttttctgta cttgtgtaat 2280

cggctaatcg ccaacagatt cggcgatgaa taaatgagaa ataaattgtt ctgattttga 2340

gtgcaaaaaa aaaggaatta agcttac 2367

<210>822

<211>45

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>822

ccaagccctc aaaaagaaga aacgattgga aaagacccaa ctaca 45

<210>823

<211>50

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>823

acgacatagc caacgaaatc acaaacgcta ttagcaatcc tgtcggattc 50

<210>824

<211>46

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>824

tgtgtctgct gatgcagttg ctactaaacc aatcaaacca gcagct 46

<210>825

<211>113

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>825

acaccctaca caaccttata tattaacaag tagtgatgat atgcttatca agctttggaa 60

ttgggaaaaa gcatgggctt gtcagcaagt tttcgaagga cacactcatt ata 113

<210>826

<211>153

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>826

tgttagtatg gatgccagtg gaggcaaaat tatttgggcc agacactctg aacttcaaca 60

ggcaaatctc aaggcgttag ctgaaggtgc ggaaataaga gatggagaac gccttccagt 120

ttctgtaaaa gatatgggtg cttgcgagat ata 153

<210>827

<211>183

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>827

gttgcttctt gctacgagct ccggtgatga aaatttagtc cgtactctag gagaaacgac 60

acaagctgaa agcaaacata acttagcatt tttgtcacac ttgttagtag gtgatttaaa 120

caaatgtcta gacattctta ttaataccgg tagattgcca gaagctgcat ttttcgccag 180

atc 183

<210>828

<211>95

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>828

ccaagccctc aaaaagaaga aacgattgga aaagacccaa ctacaacgac atagccaacg 60

aaatcacaaa cgctattagc aatcctgtcg gattc 95

<210>829

<211>96

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>829

acgacatagc caacgaaatc acaaacgcta ttagcaatcc tgtcggattc tgtgtctgct 60

gatgcagttg ctactaaacc aatcaaacca gcagct 96

<210>830

<211>266

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>830

acaccctaca caaccttata tattaacaag tagtgatgat atgcttatca agctttggaa 60

ttgggaaaaa gcatgggctt gtcagcaagt tttcgaagga cacactcatt atatgttagt 120

atggatgcca gtggaggcaa aattatttgg gccagacact ctgaacttca acaggcaaat 180

ctcaaggcgt tagctgaagg tgcggaaata agagatggag aacgccttcc agtttctgta 240

aaagatatgg gtgcttgcga gatata 266

<210>831

<211>336

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>831

tgttagtatg gatgccagtg gaggcaaaat tatttgggcc agacactctg aacttcaaca 60

ggcaaatctc aaggcgttag ctgaaggtgc ggaaataaga gatggagaac gccttccagt 120

ttctgtaaaa gatatgggtg cttgcgagat atagttgctt cttgctacga gctccggtga 180

tgaaaattta gtccgtactc taggagaaac gacacaagct gaaagcaaac ataacttagc 240

atttttgtca cacttgttag taggtgattt aaacaaatgt ctagacattc ttattaatac 300

cggtagattg ccagaagctg catttttcgc cagatc 336

<210>832

<211>141

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>832

ccaagccctc aaaaagaaga aacgattgga aaagacccaa ctacaacgac atagccaacg 60

aaatcacaaa cgctattagc aatcctgtcg gattctgtgt ctgctgatgc agttgctact 120

aaaccaatca aaccagcagc t 141

<210>833

<211>449

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>833

acaccctaca caaccttata tattaacaag tagtgatgat atgcttatca agctttggaa 60

ttgggaaaaa gcatgggctt gtcagcaagt tttcgaagga cacactcatt atatgttagt 120

atggatgcca gtggaggcaa aattatttgg gccagacact ctgaacttca acaggcaaat 180

ctcaaggcgt tagctgaagg tgcggaaata agagatggag aacgccttcc agtttctgta 240

aaagatatgg gtgcttgcga gatatagttg cttcttgcta cgagctccgg tgatgaaaat 300

ttagtccgta ctctaggaga aacgacacaa gctgaaagca aacataactt agcatttttg 360

tcacacttgt tagtaggtga tttaaacaaa tgtctagaca ttcttattaa taccggtaga 420

ttgccagaag ctgcattttt cgccagatc 449

<210>834

<211>590

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: synthetic

Primer

<400>834

ccaagccctc aaaaagaaga aacgattgga aaagacccaa ctacaacgac atagccaacg 60

aaatcacaaa cgctattagc aatcctgtcg gattctgtgt ctgctgatgc agttgctact 120

aaaccaatca aaccagcagc tacaccctac acaaccttat atattaacaa gtagtgatga 180

tatgcttatc aagctttgga attgggaaaa agcatgggct tgtcagcaag ttttcgaagg 240

acacactcat tatatgttag tatggatgcc agtggaggca aaattatttg ggccagacac 300

tctgaacttc aacaggcaaa tctcaaggcg ttagctgaag gtgcggaaat aagagatgga 360

gaacgccttc cagtttctgt aaaagatatg ggtgcttgcg agatatagtt gcttcttgct 420

acgagctccg gtgatgaaaa tttagtccgt actctaggag aaacgacaca agctgaaagc 480

aaacataact tagcattttt gtcacacttg ttagtaggtg atttaaacaa atgtctagac 540

attcttatta ataccggtag attgccagaa gctgcatttt tcgccagatc 590

<210>835

<211>657

<212>DNA

<213>Diabrotica virgifera

<400>835

gtaaattcaa attacaaacc atattagtca cccgtaaaat gtataaactc accattatta 60

caaaactttt tctttgactt ctcaaaaaca aacaatgtgg cgcttacaat gtttttcagc 120

ggacccattc aattatttta aacttatcac ttggatccga tgcacttcat caatagctaa 180

aaaaattttg ctccttgtat ctcatcttct agtataagtt cacattttct ttcattttct 240

agaacccgca aaactttaat aagtacctct tgattcattc caaaaaagat tgcatctgaa 300

acatcctctc cttgtgtgag ttcaaataat gtaaccactg tattttgcat acccctttca 360

caaatagaat catatacaat gctagcccat tcctcttggg tatgccaata gatttcccac 420

cggtttttgt ttttatctac aggagatgca tggccacttt tttgcaattc gcttagtata 480

gagattataa cgttttgttc taattttctg tttattgatg aattattaaa taaaggtgac 540

tggtttgtgt ctcttacatc tagggtgtaa gttttggttt gttcgcaaaa gtttaaaatt 600

aatgacttcc atgcatctac ttgctttgct cttgtttcag gatgtggctg caacgtg 657

<210>836

<211>1251

<212>DNA

<213>Diabrotica virgifera

<400>836

catatctctc caaactatgg cgcgttcgga ttcatttttc agacggacaa accggccttt 60

tggcgacatc tttgcattaa agctctgagc atgaactgtt tcctggacaa ggttcgcact 120

tgtctcaaaa acacctctaa atcgatgact ccacatctca atgcctctcc catgtaataa 180

atagtgtctt ctaaggcagc ttcttcagcg aatgcattca ataattgttt atataaagga 240

gctgtcgttg ttaccgcatc atctacatct atagattctt gattacttat tcgttctatg 300

gccttattta gttcttgttc tttgtcttgt agcattgtaa tatttttgtc tagatcattc 360

ttttcttttt ctaatcggct caatataccg tccaacttca attttccttg cttcaactcc 420

tcctgtgtcc tcttgagcgt ctctagttca gcttgattct gctggaattg ttccttcatt 480

cttctaagca gcttctcttc caaagctgtc atcagagatt ccctgatatg ttcttctttt 540

atagtacctg ttccggcctg aggtccaggg gggaaattaa atggatactg cggttgcgaa 600

gaagggtagg gtggatatcc ttggaaagaa ttgttgctgg ttggaggata tggagggtac 660

ccgccgaaac ctcctgatgc tggtgggtag ggggtaggat acggaggcat gtaaccacca 720

gatggttgag gcatgaatga atttgacgga tacgattcat tgtctttggc cctagcaaac 780

actggaggtt gttcgccaaa tgtaacaatc ataacttgga ttaatcctag caaatctgat 840

tcattcggca cccaatcgtg caaatatggc agataaactt ttccattttg atctacaaac 900

atggaaactt ttatggacat gtcggaagtc ggtttaacat agcaaatggg agcattctct 960

ggatgtgtgt caattaacca tatacaaatt ggaatattat aaatattgcc tttgtaacgc 1020

acaggaattg taccagttaa gttaatgaga tccattcttg tgccatcgtt gaaggtgtag 1080

acactctgtt ctggatgtaa acctctgtat tgtgtggtga tgttgattat ttctctataa 1140

gctacatcag gattgtgata agcagttagc caagtcctaa gtctctccgt atctaccatt 1200

ttctacgaat taatttcttg ccagctaaaa ctatcgagat ctagagttct c 1251

<210>837

<211>669

<212>DNA

<213>Diabrotica virgifera

<400>837

gtcagcagaa aatcgccctg agctgtatga ggaagtcaag ctatatcaca atgccaggga 60

aagagagaag tacgataatt tagcagattt gtatgctgta ataaacactt tgcaacaatt 120

agagaaggca tatattcgag attgtgtagt ggcaaaggaa tatacaggag cttgtagtaa 180

attattagtt cagtataagg ctgcgtttcg acaagtcaag agtgatgaat ttcctacacc 240

agacgccttt gttaagaagt acaggcttga ctgtcctgca gctttagaac gtatcaagga 300

agataggcca atcactatca aagacgacaa aggcaacact agtcgatgca ttgctgatat 360

cgtatcgcta ttcatcacta taatggacaa actgcggttg gacattcgtt cgatggataa 420

cttacatcct gacgtgaggg acttagtaga tactatgaac agacttagca ttctgccatc 480

ggatttcgat ggaaaggtaa aagtggctga atggttaaat actttaaatg aaatgcaggc 540

ctctgatgaa ttatccgaag ctcaagtgag gcaactgttg tttgatcttg aaagtgcgta 600

tgcgtccttt aataaagtcc ttcataattc tgcctaaatg tacgagatag ttgttatgat 660

tgttacacg 669

<210>838

<211>793

<212>DNA

<213>Diabrotica virgifera

<400>838

gatggtaggg tgacgagcag tgtgactcat ttttaatttt atattgtcaa atatttaggt 60

gttttgctgg ttattattat tatattattt aaaaatggag tggttatttg gaaaaaaagt 120

aacccccgaa gaaatgcttc gtaaaaacca acgagcatta aataaagcca tgagagattt 180

agatagagaa aagcaaaaga tggaacagca ggagaaaaaa gtaataaacg atattaaaaa 240

attagccaag gaaggtcaaa tggacgctgt caaaataatg gctaaagatc tggtcagaac 300

tagaaggtat gtgaagaaat tcatgttgat gaaggctaac attcaagcag tatcattgaa 360

aattcagact cttagatccc aaaatacaat ggcacaagct atgaaaggtg tcactaaagc 420

tatgcaaagt atgaataggc agctaaattt accacagata caaaggatat tacaagaatt 480

tgaaaagcag tctgaaatta tggatatgaa ggaagaagtt atgaatgatg ccattgatga 540

tgcgatggaa ggagatgatg atgaggagga aactgacgca gttgtcaatc aagttttaga 600

tgaattgggt cttcaattga atgatactct ttctggactg cctcaaaccg ctggatctct 660

tcctgcctct gggcaaaaac atcctgcagc tgcagctgct gttgcaggag gaggtaatgg 720

tggcactggt actggaggca gtggtggaaa cagtggcggc ggaggtggcc tttctgatgc 780

cgatgcggat ttg 793

<210>839

<211>767

<212>DNA

<213>Diabrotica virgifera

<400>839

gctacctcct cttcatcctc tgattcttcc tcattgactt ccactttagg ttcttctgaa 60

ggcacggaag gcatctcttg ctcaatcata ccagccaatt cttcttctac agctgcttca 120

tcttcatctg tcaattgacc actaagaagt tcgttaatct cattctgttt gtcaacccct 180

tctcttgttt catccaatat acgttcaata tcttcaattt tgagggaatc attaatttgt 240

ttcaaagctt cgttgcctgc ttttagacca ctcactactt gagtttctaa ctgggcgaac 300

tctatatcac tagccatttt atcgagatta tcaagttgta catctagctt ggacaacagc 360

tgttcttgat acctcttctt cttaagtaaa agttttgctc tgtctctttg gccattgtta 420

agtaacttct tagctatatc tttatcttta ccaagtgaga gctcaattct tttttggtat 480

tgtctcaatt tatctctttg ctgtttgagc tgtaacactg ctttatcgtg ttctgttact 540

ctggagggag gctttttttt actaaaaaag atacccattt tattacttta gtaaaatata 600

aaatttctct tgatttgtgt tattcaaaaa attgatacca tccttatatt atagttttcc 660

tattatcaca cgttttatgc tgttttctta tatcttttag gactaatttt taatcagggt 720

tgccagatga aatttcagaa tatccctaga cgggagcttc aatttcc 767

<210>840

<211>588

<212>DNA

<213>Diabrotica virgifera

<400>840

ctgcttcaaa gaaggaccac atgactaccc tagaatttgt gctttcattg atgcttctat 60

gaattctgtc tctaacctgc atatattctt gttcttgttt tacaccagac aatgaatttg 120

taagttctct gatcatttcc tccaacttat tagcattttc accttcttgt tgttctgcct 180

ttggagattc cccaacagcc atattaaaca taacaacttt aggtgtcata gttgacatct 240

tgtttgagaa acaatatgta tatataccag atgtgtgggc tgcaaatgtg tattttccgg 300

cagtttccct ttcaccctgg tataagttct ggccgtttgg atctataata cgcacatcta 360

tgtcaaggaa tcctccttct gctatttcaa aagtgagacc catttttgtg ccagcttcca 420

ctttgtcaaa gaaacattct tctgcatgtg catctactgt gataaaatag gccaaacttt 480

gatttatgcc caaacaaacc agtgaaataa ggaagccgaa aagatacatg tttactttaa 540

attaaaatta gaaaacttcg gccacgtaac ctctcacact tgacgttc 588

<210>841

<211>1098

<212>DNA

<213>Diabrotica virgifera

<400>841

cctgtacttt cctgtatcct catgttcact gtctaccgtt ttactcactt cctttgttaa 60

tactaatacc atttcttcta tgttccgtga agagactaat tcaagggcta gacttaaagt 120

cttcttgcgg acttctaagt caggagcaga gagtactctc agtatgtcca taactaaatc 180

ctgcagaatt ctttcgtgat taggaagctc cttaagtgct atcagcctgt ccaaaacgat 240

gagttttaca ttgttgtcac tttctttgat aattaactca atgtaacagc tagcagcagc 300

tttaacggca gtcggggcac tggagagggt gactaaagtt cctgcagctt cgtacctgac 360

agcaggactg cttgagttca acaagttata tatacatcta ataaatctag atctttccgc 420

aggattggaa tgacacacct tatatatcaa ctcaacgatg accagttgta gaatatctcc 480

aaatgaattt acttgatcta aacatgatgc caaatacgac aacgcccttt cttggtcagc 540

atgaagaagc attaaaaacg catttctttt acaagacatg tcttgctcac catccaaata 600

attggagatc agttcaggag catctggaat gagggcttca aaatttttgt aaatggtaaa 660

aattgccagt acagcattcc tcctcacata gctgtgccta tgatccaaac aagctctaat 720

actgggcatt aatggttcca acaattctgg ttccttcagt ttgcacaaga agcgaagtgt 780

agaacctctc aaaaattcat ttgggtgttg cagatctttt ctataggcat cacataccaa 840

aatcatctct tgtagtagtt taccttctgg atttgttttt ggaactattt cccagaaaat 900

caataatagt ttttttgcca aatgatcttg tagaggtaaa acgtttctta taatggtcat 960

taggaatcca ttcggcaacc tttctcctgc cagaatcatg tgaattattt tttctaatgc 1020

tcttattttt actttaacct cacccttttc taaatccatt tttagttgca tttcattgta 1080

gggctccgaa tctgttgg 1098

<210>842

<211>718

<212>DNA

<213>Diabrotica virgifera

<400>842

ccttgttctt gattggaact gggttttgtt tcatttgcag aacgatggaa cgcattcgag 60

aaacgtcttt gctgccttgt tttgctttag gattgaaatc acacaatttt gctattcttt 120

cccattcggt tcccggttct agttcgtcat cttcggctac gaactgcttt tcagcgttcc 180

tattagcagc ttttgtcttg gctatagctt cttcgtgatt cttgtaccaa tcttctaatt 240

ctttctttgc tacttccctc aattcttgct tctttttctc ttcttcctga tctttttcct 300

ccaacctctt gatttgctct tccctccata acctgatctt ttctggttct actctatctt 360

cttgtcttat tgtaagtggg gacactcttt ccctgtattc tttatctgga tcaaaatcgg 420

ctgggatatc attaaacttt ggctcttcca aaaaaccatc tggaccattg ttttctacca 480

tttcaaaact gctcgcagaa ttggttgggt catcaccatt aagaactgga gcaataactg 540

cggccggttt cacttcatct tcaaggcctg ctagttcgtc ttgctcgcgt gccaagaatt 600

ctgccgctgg gtcttcgtcg gcaactccaa aattgtctcc aaatccagac attcttgatt 660

ttgttaatct taaggataag aaaagctgct attttcccac tgacactgac agcaaagc 718

<210>843

<211>842

<212>DNA

<213>Diabrotica virgifera

<400>843

caatcaagct ggaatggtag aaactgaagg ggaatattgc tggcaaatcc tgtgaaaatc 60

attttctttt atcttcagat cacgaatgta tggatgatca ggatttgcat ccttcaactg 120

agacagatat ctattagcaa tctcaggtgc tttggccata tgtctctgaa gagtgatcat 180

gttaacaagt gtatcaggat agttggcatc cttatcaagt gcttcttgaa gagctgcttc 240

ggcttcctca tatttggctt ggccaatgaa agtcactgct tgtccgttta gcaacaatgg 300

agtggatcca tatttgtcta caagttcttg gtaaatatag taggcgtcct ggagtttatc 360

tccaccagtg gatacattaa tccaagcttg agccagttgt gttaaagttg catcatcgtc 420

cttatcttgc atctctttca gcttcttcct tgccaaatct actctatcca atttcaaaag 480

tatatctata ataaaggcca ttgcttcgag gctttctgta gcgtgcaaaa ctttatacgc 540

agcatcaaga ttgccttcgt taacataaat agtagctgct accaaaatta gagtatcatt 600

gttagcattt gcttggactt gttgctccac ttgagccacc acagaatcct tcttgatttt 660

gcctgctaag tagtcagcga ggtatttcag gggttggatt tctgggggag atgccccgtg 720

gatttcgtct aatactacta ggaatttatt ttgggccata taggaccggt aggtgtagaa 780

gtctctttgg actgctactt cgggcgttga cggctttctg agtttttgtg cctcgtttat 840

gc 842

<210>844

<211>679

<212>DNA

<213>Diabrotica virgifera

<400>844

cttctgcaaa agatttggca tacgagatca tgtgcaggta tatcaggaaa aagtcagatc 60

ctctttgcta tagtctacac tacaagatac cttgacttag tgacgtcata tgtatctatg 120

tacaatactg ttatgaaatt tgtatttttg gccacatcgt atgcaactat ttatttaatc 180

tacatgaaat ttaaagccac ttatgaccat aaccatgaca cgttcagaat agagtttctc 240

attttaccat cgtttggttt ggctatgctt atcaatcacg agttttcatt tatggaggtc 300

ttgtggactt tcagtattta tttggaatca gtggctatat tgccgcaatt gttcatggtc 360

agtaaaacag gagaagcaga aagtattact tcgcactact tattcgcatt gggatcttat 420

agggccctat acattttgaa ctggatctac cgctatgcaa ctgaaactca ttacgatctc 480

atcgctatag tagctggtgt tgtgcagact atattatatt gtgacttctt ttatctttac 540

attactaaag tactcaaggg aaagaaacta caattgcccg cttaaacccc tcttatataa 600

gacttaactg tacaaacatt ttactttcag ctaaacggta ttaacacaat gatgcctaat 660

gatcatgtgt ttggatagc 679

<210>845

<211>725

<212>DNA

<213>Diabrotica virgifera

<400>845

atgtataaga gggatcgggc cagaaagctg cagataatgc ggcagcggca gttggcggcg 60

caaacgttga ggggcggatt agggcccgat atgtattccg gacaaccggg cacttcgcct 120

ttcgccaaca tccacatcaa acaagaaata caaatacctc aagtgtcctc ccttacctca 180

tcgccagatt cgtcgcccag tcccatagca gtagctttag gccaggtgaa ctcctcgttg 240

gtgcagccca cgtccaatca gcagcccgct ctccagatcg tcggcgttcc tggaggtcac 300

tcgtcgatgg tcatgggccc ggataacaaa ctctgggccc aagcgaactc caccaccaca 360

tcacccctta gtcccaaagt gttccaattc gagactgtcg taccgggcag cagtgtaccg 420

ccttcgagta aagtgtcgcc gctcattagg gatttcgttg ccgccgttga cgatcgcgaa 480

tggcaaaact ctttgtacac tcttttacaa aaccagacgt acaatcagtg tgaagtggac 540

ttatttgaac taatgtgtaa agttttggac caaaatctct tttctcaagt cgattgggcg 600

aggaattcga tattctttaa agatctcaag gtggacgatc aaatgaaact tctgcagcat 660

tcatggtcgg atatgttggt gctggatcac atacaccagc gcacgcacaa taacctaccc 720

gacga 725

<210>846

<211>864

<212>DNA

<213>Diabrotica virgifera

<400>846

gcgtgggcgt ggcttataat gctagtatag gaggtgtacg tatgttagat ggtgtagtaa 60

acgacgcagt tgaagccaga gccctaggtc taaacccgga acacatcgac atctacagcg 120

cttcctgggg acctgaagac gacgggaaaa ccgtagacgg tcccggacca ttagcaagac 180

gagcttttat atacggagta actagtggga gaaaaggaaa aggatctatt ttcgtttggg 240

cttcgggaaa tggaggccga catacggatt cctgcaattg tgatggatac accaacagta 300

tctttacctt gagtatatct agtgctacac aaggaggtta caaaccttgg tatttggaag 360

agtgttcttc aacattagca acaacttaca gctcaggaac acctggacat gacaaaagcg 420

tcgcgactgt tgatatggac gctagattac gaccagatca tatctgtact gtagaacata 480

ctggaacttc agcttcagca cctttagctg ctggaatatg cgccctagct cttgaagcca 540

accccacgtt gacgtggcgt gacatgcaat atttagtagt actaacttca agagctcctc 600

cactggaaaa agaggctgga tggatagtaa acggcgttgg gcgaaaagct tctcataaat 660

ttggttatgg tctcatggac gctggtgctt tggttacttt agctgaaaaa tggactacgg 720

taccacctca acacatctgt aaaagtcagg aaattgtaga agatcgacca attaatcctg 780

cctatggttc cagtttgaat ttcaatatgg aagtagacgc atgtagtggt accttgaacg 840

aagtcaggta cctggagcac gtgc 864

<210>847

<211>140

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.1 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv26-Dv49 order is formed

<400>847

catcacgact atcctactag tggagaagta tgcgaagtcg atgtcagaga ttgggaagaa 60

tgcaacaggg ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt 120

tgctactaaa ccaatcaaac 140

<210>848

<211>210

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.2 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv26-Dv49-Dv2 3 orders is formed

<400>848

catcacgact atcctactag tggagaagta tgcgaagtcg atgtcagaga ttgggaagaa 60

tgcaacaggg ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt 120

tgctactaaa ccaatcaaac gatgaggaac agacagaaca aaccgcaact gaaacggcaa 180

ctacagaggt acagaaaaag ggtgtcaagg 210

<210>849

<211>280

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.3 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv26-Dv49-Dv23-Dv20 order is formed

<400>849

catcacgact atcctactag tggagaagta tgcgaagtcg atgtcagaga ttgggaagaa 60

tgcaacaggg ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt 120

tgctactaaa ccaatcaaac gatgaggaac agacagaaca aaccgcaact gaaacggcaa 180

ctacagaggt acagaaaaag ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca 240

cacggtgctg cacaattctt aagagagagg ctgtttgaag 280

<210>850

<211>350

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.4 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv26-Dv49-Dv23-Dv20-Dv13 order is formed

<400>850

catcacgact atcctactag tggagaagta tgcgaagtcg atgtcagaga ttgggaagaa 60

tgcaacaggg ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt 120

tgctactaaa ccaatcaaac gatgaggaac agacagaaca aaccgcaact gaaacggcaa 180

ctacagaggt acagaaaaag ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca 240

cacggtgctg cacaattctt aagagagagg ctgtttgaag tctcttcagc aatagacaat 300

ttgactccct ttcctcttgg gagggaaacg aaggccttag ctcccttacc 350

<210>851

<211>420

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.5 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv26-Dv49-Dv23-Dv20-Dv13-Dv22 order is formed

<400>851

catcacgact atcctactag tggagaagta tgcgaagtcg atgtcagaga ttgggaagaa 60

tgcaacaggg ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt 120

tgctactaaa ccaatcaaac gatgaggaac agacagaaca aaccgcaact gaaacggcaa 180

ctacagaggt acagaaaaag ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca 240

cacggtgctg cacaattctt aagagagagg ctgtttgaag tctcttcagc aatagacaat 300

ttgactccct ttcctcttgg gagggaaacg aaggccttag ctcccttacc tcatcgtccg 360

tttggattca caaaaacaca tcgacttctc tctgaaatca cctttgggag gcggtcgtcc 420

<210>852

<211>140

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.6 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv49-Dv23 order is formed

<400>852

ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt tgctactaaa 60

ccaatcaaac gatgaggaac agacagaaca aaccgcaact gaaacggcaa ctacagaggt 120

acagaaaaag ggtgtcaagg 140

<210>853

<211>210

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.7 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv49-Dv23-Dv20 order is formed

<400>853

ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt tgctactaaa 60

ccaatcaaac gatgaggaac agacagaaca aaccgcaact gaaacggcaa ctacagaggt 120

acagaaaaag ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca cacggtgctg 180

cacaattctt aagagagagg ctgtttgaag 210

<210>854

<211>280

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.8 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv49-Dv23-Dv20-Dv13 order is formed

<400>854

ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt tgctactaaa 60

ccaatcaaac gatgaggaac agacagaaca aaccgcaact gaaacggcaa ctacagaggt 120

acagaaaaag ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca cacggtgctg 180

cacaattctt aagagagagg ctgtttgaag tctcttcagc aatagacaat ttgactccct 240

ttcctcttgg gagggaaacg aaggccttag ctcccttacc 280

<210>855

<211>350

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.9 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv49-Dv23-Dv20-Dv13-Dv22 order is formed

<400>855

ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt tgctactaaa 60

ccaatcaaac gatgaggaac agacagaaca aaccgcaact gaaacggcaa ctacagaggt 120

acagaaaaag ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca cacggtgctg 180

cacaattctt aagagagagg ctgtttgaag tctcttcagc aatagacaat ttgactccct 240

ttcctcttgg gagggaaacg aaggccttag ctcccttacc tcatcgtccg tttggattca 300

caaaaacaca tcgacttctc tctgaaatca cctttgggag gcggtcgtcc 350

<210>856

<211>420

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.10 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv49-Dv23-Dv20-Dv13-Dv22-Dv18 order is formed

<400>856

ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt tgctactaaa 60

ccaatcaaac gatgaggaac agacagaaca aaccgcaact gaaacggcaa ctacagaggt 120

acagaaaaag ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca cacggtgctg 180

cacaattctt aagagagagg ctgtttgaag tctcttcagc aatagacaat ttgactccct 240

ttcctcttgg gagggaaacg aaggccttag ctcccttacc tcatcgtccg tttggattca 300

caaaaacaca tcgacttctc tctgaaatca cctttgggag gcggtcgtcc gatccttttc 360

gatttgttgc tgccattggg gatccatctg gacttctgga ggaagcaagg caggtgcagc 420

<210>857

<211>140

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.11 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv23-Dv20 order is formed

<400>857

gatgaggaac agacagaaca aaccgcaact gaaacggcaa ctacagaggt acagaaaaag 60

ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca cacggtgctg cacaattctt 120

aagagagagg ctgtttgaag 140

<210>858

<211>210

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.12 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv23-Dv20-Dv13 order is formed

<400>858

gatgaggaac agacagaaca aaccgcaact gaaacggcaa ctacagaggt acagaaaaag 60

ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca cacggtgctg cacaattctt 120

aagagagagg ctgtttgaag tctcttcagc aatagacaat ttgactccct ttcctcttgg 180

gagggaaacg aaggccttag ctcccttacc 210

<210>859

<211>280

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.13 concatermer of highly effective WCR target, about 50%GC standard is by 5_3 '

For the fragment of Dv23-Dv20-Dv13-Dv22 order is formed

<400>859

gatgaggaac agacagaaca aaccgcaact gaaacggcaa ctacagaggt acagaaaaag 60

ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca cacggtgctg cacaattctt 120

aagagagagg ctgtttgaag tctcttcagc aatagacaat ttgactccct ttcctcttgg 180

gagggaaacg aaggccttag ctcccttacc tcatcgtccg tttggattca caaaaacaca 240

tcgacttctc tctgaaatca cctttgggag gcggtcgtcc 280

<210>860

<211>350

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.14 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv23-Dv20-Dv13-Dv22-Dv18 order is formed

<400>860

gatgaggaac agacagaaca aaccgcaact gaaacggcaa ctacagaggt acagaaaaag 60

ggtgtcaagg ggtgagatgg aacgagattg tcaaatttca cacggtgctg cacaattctt 120

aagagagagg ctgtttgaag tctcttcagc aatagacaat ttgactccct ttcctcttgg 180

gagggaaacg aaggccttag ctcccttacc tcatcgtccg tttggattca caaaaacaca 240

tcgacttctc tctgaaatca cctttgggag gcggtcgtcc gatccttttc gatttgttgc 300

tgccattggg gatccatctg gacttctgga ggaagcaagg caggtgcagc 350

<210>861

<211>140

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.15 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv20-Dv13 order is formed

<400>861

ggtgagatgg aacgagattg tcaaatttca cacggtgctg cacaattctt aagagagagg 60

ctgtttgaag tctcttcagc aatagacaat ttgactccct ttcctcttgg gagggaaacg 120

aaggccttag ctcccttacc 140

<210>862

<211>210

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.16 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv20-Dv13-Dv22 order is formed

<400>862

ggtgagatgg aacgagattg tcaaatttca cacggtgctg cacaattctt aagagagagg 60

ctgtttgaag tctcttcagc aatagacaat ttgactccct ttcctcttgg gagggaaacg 120

aaggccttag ctcccttacc tcatcgtccg tttggattca caaaaacaca tcgacttctc 180

tctgaaatca cctttgggag gcggtcgtcc 210

<210>863

<211>280

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.17 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv20-Dv13-Dv22-Dv18 order is formed

<400>863

ggtgagatgg aacgagattg tcaaatttca cacggtgctg cacaattctt aagagagagg 60

ctgtttgaag tctcttcagc aatagacaat ttgactccct ttcctcttgg gagggaaacg 120

aaggccttag ctcccttacc tcatcgtccg tttggattca caaaaacaca tcgacttctc 180

tctgaaatca cctttgggag gcggtcgtcc gatccttttc gatttgttgc tgccattggg 240

gatccatctg gacttctgga ggaagcaagg caggtgcagc 280

<210>864

<211>140

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.18 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv13-Dv22 order is formed

<400>864

tctcttcagc aatagacaat ttgactccct ttcctcttgg gagggaaacg aaggccttag 60

ctcccttacc tcatcgtccg tttggattca caaaaacaca tcgacttctc tctgaaatca 120

cctttgggag gcggtcgtcc 140

<210>865

<211>210

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.19 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv13-Dv22-Dv18 order is formed

<400>865

tctcttcagc aatagacaat ttgactccct ttcctcttgg gagggaaacg aaggccttag 60

ctcccttacc tcatcgtccg tttggattca caaaaacaca tcgacttctc tctgaaatca 120

cctttgggag gcggtcgtcc gatccttttc gatttgttgc tgccattggg gatccatctg 180

gacttctgga ggaagcaagg caggtgcagc 210

<210>866

<211>140

<212>DNA

＜213〉artificial

＜220〉artificial sequence note: the C6.20 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv22-Dv18 order is formed

<400>866

tcatcgtccg tttggattca caaaaacaca tcgacttctc tctgaaatca cctttgggag 60

gcggtcgtcc gatccttttc gatttgttgc tgccattggg gatccatctg gacttctgga 120

ggaagcaagg caggtgcagc 140

<210>867

<211>70

<212>DNA

<213>Diabrotica virgifera

<400>867

catcacgact atcctactag tggagaagta tgcgaagtcg atgtcagaga ttgggaagaa 60

tgcaacaggg 70

<210>868

<211>70

<212>DNA

<213>Diabrotica virgifera

<400> 868

ctccaagtgc caggtccaac tcaactgccg gctgtgtctg ctgatgcagt tgctactaaa 60

ccaatcaaac 70

<210>869

<211>70

<212>DNA

<213>Diabrotica virgifera

<400>869

gatgaggaac agacagaaca aaccgcaact gaaacggcaa ctacagaggt acagaaaaag 60

ggtgt caagg 70

<210>870

<211>70

<212>DNA

<213>Diabrotica virgifera

<400>870

ggtgagatgg aacgagattg tcaaatttca cacggtgctg cacaattctt aagagagagg 60

ctgtttgaag 70

<210>871

<211>70

<212>DNA

<213>Diabrotica virgifera

<400>871

tctcttcagc aatagacaat ttgactccct ttcctcttgg gagggaaacg aaggccttag 60

ctcccttacc 70

<210>872

<211>70

<212>DNA

<213>Diabrotica virgifera

<400>872

tcatcgtccg tttggattca caaaaacaca tcgacttctc tctgaaatca cctttgggag 60

gcggtcgtcc 70

<210>873

<211>70

<212>DNA

<213>Diabrotica virgifera

<400>873

gatccttttc gatttgttgc tgccattggg gatccatctg gacttctgga ggaagcaagg 60

caggtgcagc 70

<210>874

<211>1358

<212>DNA

<213>Diabrotica virgifera

<400>874

cttgttgaat tggaggaggt ggcttcaaaa attttgcatt tggatctaca tctggaaaac 60

ctttcttttc tccaatttgt tccttcagct gttcagcttc atctaccatt ccgtgagtag 120

cagctgtcaa gtaggccaaa gaaagttgat tggaagcctt caaaatttta acacgctctt 180

caagatcacc aagcaaaagt gcagcatgat attgagatga tttagctttt ctaatttccg 240

caattttcat catttttctc aatttgtcca ggttgccggt aatcagatat aggaatgata 300

atttttcgaa actcttggtc ctttgataac acatctcaac tacttgatgg tttccttgcc 360

atagagcagc atgtgctagt tgatcccaac aagctttgtc attcaaagat ttcgcagcat 420

ctagagcaat ctcaatattt ccacattcca gagccaaagt aaatcgagtt ttgtcgtctt 480

ttacaaaatg taaggctact tcaggataac ctttttgttg taagtaagaa atcatagatt 540

gtcccaccaa ttttgaattt ctaaccatat acaaaacttc ttcatatttg tgattaatta 600

atgcaagttt gaatttgtat tccattgaat ccactgtcaa tacccgtggt ttgcattcac 660

gatcaaggca aaagatctgg tttcctctta ctctggtgat gtatattgga agatcgaggg 720

ttctaataat tccttggtca ccgtttttaa gtgtatattt caaaatggtt gcttgtagta 780

taaataaaaa ctcctgtatc atcccaagct ccagatttca ctctagtatt ttcgtgtaaa 840

caacataaaa catccaattt cctattacaa atagtcacgt tgtgtttgga aaggagagcc 900

aagtgggaca tatcagacga ccagacaacg tatctgcatt tgttaatttt cacttgtgct 960

aaagttctct tctgttgaac gtcaaacaga gtaacatgtt cagaatctct taataacagc 1020

attccagtac cagcataaaa tacttcatca catgtaggag tttgtacctt cttggtgact 1080

tcgtttttca agttcttgat aattagttga tgagtcctgt ccaaaacggc aaatctgttt 1140

cttgcaaccc ataatgccgt aagtccagaa gaccttttgc tttcagcttc tggtacttga 1200

tcatctctct cctgctcttt tggaatcaca tataaatcgt atgtgctatt atccaagttt 1260

gaagttcttg tagaaagaag aacagcattt tcagctggat taaaggacat cttataaact 1320

ggtatttttc ctccaccacg aatctgaatg actggaac 1358

<210>875

<211>2714

<212>DNA

<213>Diabrotica barberi

<400>875

atgccacttc gattagatat aaaaagaaag ctaacagctc gctcagaccg ggtaaaatgt 60

gtggatcttc accctacaga accttggatg ctatgttctc tttacagcgg aaatataaac 120

gtttggaaca ccgaaaatca gcaactggtt aagacttttg aagtatgtga tgtacccgtt 180

cggacagcta agtttttgcc caggaagaac tggatagtca gtgggtctga tgatatgcag 240

attcgagttt tcaattacaa taccttagat cgggtacatt cttttgaggc tcattcggat 300

tatgtgagat gtattgtcgt acaccctaca caaccttata tattaacaag tagtgatgat 360

atgcttatca agctttggaa ttgggaaaaa gcatgggcct gtcagcaagt tttcgaagga 420

cacactcact atattatgca aatcgccata aatccaaaag acaacaacac atttgccagt 480

gcatccctag acagaacatt gaaagtatgg caattgggag cgtccacagc caatttcaca 540

ctagaaggtc acgagaaagg cgttaactgt gtggactatt atcatggtgg agataagcct 600

tatttaatct caggtgctga tgatagatta gtaaaaatct gggattatca aaacaaaact 660

tgtgttcaaa ctttagaagg acatgctcaa aatgtaactg ctgcatgttt ccatccagaa 720

cttcctgtag ctcttactgg aagtgaagat ggtactgtca gagtgtggca tgccaacacc 780

catagattag aaagtagctt aaattatggt tttgaaagag tgtggactat tttctgccta 840

aagggatcca ataacgtggc attgggttat gatgaaggta gcattttggt taaagttggt 900

agagaagaac cagctgttag tatggatgcc agtggaggca aaattatttg ggccagacac 960

tctgaacttc aacaggcaaa tctcaaggcg ttagctgaag gtgcggaaat aagagatgga 1020

gaacgccttc cagtttctgt aaaagacatg ggtgcttgcg agatataccc tcagacaatt 1080

caacacaatc ccaatggccg ttttgtcgtt gtctgtggag atggagaata cataatctac 1140

acagcaatgg ctttaagaaa caaagccttt ggtagtgcac aagaattcgt gtgggctcaa 1200

gattccagcg aatatgccat cagagaatcc ggatctacta tcagaatttt taagaatttc 1260

aaagagaaga agaattttaa gtccgacttt ggagctgaag gtatatacgg tggatacctt 1320

ttgggagtca aatcagtttc tggtttgact ttctacgatt gggaaactct tgatttagta 1380

agaagaatcg agatacaacc aaaagcagtt tactggtcag atagtggtaa attagtatgt 1440

ttggctacag aagatagcta ctttattctt tcttatgatt ccgatgaagt tcaaaaagcc 1500

agagataaca atcaggttgc ggatgatgga gtagaatcgg ctttcaatct tctaggtgaa 1560

ataaacgaat cagtgcgaac tggtctctgg gtaggcgact gttttattta cacgaattct 1620

gttaatcgta tcaactactt cgttggaggt gaactggtta caattgccca tttggatcgg 1680

cctttatatg tcttgggata tgtgcctaaa gacgatagat tatacctcgt agataaagag 1740

ttgcgcgtag taagctacca attacttctc tccgttcttg aataccaaac tgccgtcatg 1800

agaagagact ttccaacagc cgatagagta cttccgtcca ttcctaagga gcacagaacg 1860

agagtcgcac atttcttaga aaagcaaggc ttcaaacagc aagctttggc cgtaagtaca 1920

gatccagagc acagattcga gctggcagta gcattagagg atcttaacac agccaaaatt 1980

ctagctcaag aagcaaacag tcctcaaaag tggaatcaac tagcagaatt ggctgccgct 2040

actaataatg taagcttagc caaggaatgt atgcaaaaag cgcaagatta tggaggctta 2100

ttgcttcttg ctactagttc aggggatgaa aatttagtcc gtactctagg agaaacgaca 2160

caagcagaaa gcaaacataa cttagcattt ttatcacact tgttagtagg tgatttaaac 2220

aaatgtctag acattcttat taataccggt agattgccag aagctgcatt tttcgccagg 2280

tcttaccttc ctgataagat tacggaagtc gtagaacttt ggaagactca gttgtcttca 2340

gtcaatcaaa aagctggaca gagccttgcc gatcctaaaa actacgaaaa tctgttccct 2400

ggtttacaag aggcggtggt agctcagaaa tttttggaac agcagaataa aggtttagca 2460

cctgcgagaa ttgccaccac cattcctcct aatcacgaca ggaatgttgt agcagaagtt 2520

caagcacaat cgaaactcga cataccatca tttagttctt catttatttc atcagaaata 2580

gaagcacaaa caaggagttc tgctaaacct gaagagtctt caaacattat acagctggac 2640

caagatgacg acgatatcga tttagatttg gacggtgtaa atatcgatga aaacattgac 2700

acgacggata tcaa 2714

<210>876

<211>2669

<212>DNA

<213>Diabrotica balteata

<400>876

taacagctcg ctcagaccgg gtaaaatgtg tggatcttca ccctacagaa ccttggatgc 60

tatgttccct gtacagtgga aatatcaatg tttggaatac cgaaaatcaa caactggtta 120

aaacttttga agtatgtgat gtacctgtac gaacagctaa gtttttaccc agaaagaact 180

ggatagtcag tggatctgat gatatgcaga ttcgagtttt caattacaat accttagatc 240

gggtacattc ttttgaagct cattcagatt atgtgagatg tattgtcgtt catcctacac 300

aaccttatat attaacaagt agtgatgata tgcttatcaa gctttggaat tgggaaaaag 360

catgggcttg tcagcaagtt tttgaaggac acactcatta tattatgcaa atcgccataa 420

atccaaaaga caacaacaca tttgccagtg catctttaga tagaacattg aaagtatggc 480

agttgggagc atcaacagcg aatttcacac tagaaggtca cgagaaaggt gtaaactgtg 540

tggattatta tcatggtgga gataagcctt atttaatctc aggtgctgat gatagattag 600

taaaaatctg ggattatcaa aataaaactt gtgttcaaac tttggaagga catgctcaaa 660

atgtaacggc tgcttgtttc catccagaac ttcctgtagc ccttactgga agtgaggatg 720

gtactgtcag agtgtggcat gccaacaccc atagattaga gagtagctta aattatggtt 780

ttgaaagagt atggactatt ttctgcctaa agggatcaaa taatgtggca ttgggttatg 840

atgaaggtag cattttggtt aaagttggta gagaagaacc agctgttagt atggatgcca 900

gtggaggtaa aattatttgg gctagacact ctgaacttca acaggcaaat ctcaaggcat 960

tagctgaagg tgcagaaata agagacggag aacgccttcc agtttctgta aaagatatgg 1020

gagcttgcga gatataccct cagacaattc aacacaatcc taacggccgt tttgtcgttg 1080

tatgtgggga tggagaatac ataatctaca cagcaatggc tttaagaaac aaagcttttg 1140

gtagtgcaca agaatttgtg tgggctcaag attccagcga atatgccatc agagaatccg 1200

gatccacaat cagaattttt aagaatttca aagagaagaa gaattttaag tccgactttg 1260

gagctgaagg tatatatggt ggttaccttt tgggagtcaa atctgtttct ggtttgactt 1320

tctatgattg ggaaactctc gatttggtaa gaagaatcga gatacagcca aaagcagttt 1380

actggtcgga tagtggcaaa ctagtatgtt tggctacaga agacagctac tttattcttt 1440

cttatgattc cgatgaagtt caaaaagcca gagataacaa tcaggttgcc gatgatggag 1500

tagaatcagc tttcaatctt ctaggtgaaa taaacgaatc agttcgaact ggtctgtggg 1560

taggcgactg ttttatctac acaaattctg ttaatcgtat caactatttt gttggaggtg 1620

aactagttac aattgcccat ttggatcggc ctttgtatgt cttaggatat gtgcccaaag 1680

atgatagatt atacctggta gataaagagt tgcgcgtagt aagctaccaa ttacttctct 1740

ctgttcttga atatcaaacc gctgtcatga gaagagactt tccaacagca gatagagtac 1800

ttccgtctat tcctaaggag cacagaacga gagtcgcaca tttcttagaa aagcaaggct 1860

tcaaacaaca agctttggcc gtaagtacag atccagagca cagattcgag ctggcagtag 1920

cattagagga tcttaacaca gccaagattc tagctcaaga agcaaacagt ccgcaaaagt 1980

ggaatcaact agcagaattg gctgccgcta ctaataacgt aagcctagcc aaggaatgta 2040

tgcagaaagc gcaagattat ggcggcttat tacttcttgc tacaagttcc ggggatgaaa 2100

gtttagtccg tactctagga gaaacgacac aagcagaaag caaacataac ttagcatttt 2160

tgtcacactt gttagtaggt gatttaaaca aatgcctaga cattcttatt aataccggta 2220

gattaccaga agctgcattt ttcgccagat cttatcttcc tgataagata acggaagtgg 2280

tagaactttg gaagactcag ttatcttcag tcaatcaaaa agccggacag agccttgccg 2340

atccgaaaaa ttacgaaaat ctgttccctg gtttacaaga ggcagtggta gctcagaaat 2400

ttttggaaca gcagaataaa ggtttagcac ccgcgcgaat tgccaccacc attcctccta 2460

atcatgacag gaatgttgta gccgaagttc aagcacaatc gaaacatgat ggaccatcat 2520

ttagttcgag gagtatttca tcagaaatag aagcacaaac aaggagttct gctaaacctg 2580

aagaatcttc aaacattata cagctggatc aagatgacga cgatatcgat ttagatttgg 2640

acggtgtaaa tatcgatgag aacattgac 2669

<210>877

<211>2701

<212>DNA

<213>Diabrotica undecimpunctata howardii

<400>877

atgccacttc gattagatat aaaaagaaag ctaacagctc gctcagaccg ggtaaaatgt 60

gtggatcttc accctacaga accttggatg ctatgttctc tttacagtgg aaatatcaac 120

gtttggaata ccgaaaatca acaactggtt aaaacttttg aagtatgtga tgtacctgta 180

cggacagcta agtttttgcc caggaagaac tggatagtca gtggatctga tgatatgcag 240

attcgagttt tcaattacaa taccttagat cgggtacatt cttttgaagc tcattcagat 300

tatgtgagat gtattgtcgt acatcctaca caaccttata tattaacaag tagtgatgat 360

atgcttatca agctttggaa ttgggaaaaa gcatgggctt gtcagcaagt ttttgaagga 420

cacactcatt atattatgca aatcgccata aatccgaaag acaacaacac atttgccagt 480

gcatctttag atagaacatt gaaagtatgg cagttgggag catcaacagc taatttcaca 540

ctagaaggtc acgagaaagg tgttaactgt gtggattatt atcatggtgg agacaagcct 600

tatttaatct caggtgctga tgatagatta gtaaaaatct gggattatca aaataaaact 660

tgtgttcaaa ctttggaagg acatgctcaa aatgtaacag ctgcatgttt ccatccggaa 720

cttcctgtag cccttactgg aagtgaggat ggcactgtca gagtgtggca tgccaacact 780

catagattag agagtagctt aaattatggt tttgaaagag tatggaccat tttctgccta 840

aagggatcaa ataatgtggc attgggttat gatgaaggta gcattttggt taaagttggt 900

agagaagaac cagctgttag tatggatgcc agtggaggta aaattatttg ggctagacac 960

tctgaacttc aacaggcaaa tctcaaggca ttagctgaag gtgcagaaat aagagatgga 1020

gaacgccttc cagtttctgt aaaagatatg ggagcttgcg agatataccc tcagacaatt 1080

caacacaatc ctaacggccg ttttgtcgtt gtctgtggag atggagaata cataatctac 1140

acagcaatgg ctttaagaaa caaagcgttt ggtagtgcac aagaatttgt gtgggctcaa 1200

gattccagcg aatatgctat cagagaatcc ggatctacta tcagaatttt taagaatttc 1260

aaagagaaga agaatttcaa gtccgacttt ggagctgaag gtatatatgg tggttacctt 1320

ttgggagtca aatctgtttc tggtttgact ttctatgatt gggaaactct cgatttggta 1380

agaagaatcg agatacaacc aaaagcagtt tactggtccg atagtggcaa actagtatgt 1440

ttggctacag aagacagcta ctttattctt tcttatgatt ccgatgaagt tcaaaaagcc 1500

agagataaca atcaggttgc tgatgatgga gtagaatcgg ctttcaatct tctaggtgaa 1560

ataaacgaat cagttcgaac tggtctgtgg gtaggcgact gttttatcta cacgaattct 1620

gttaatcgta tcaactattt tgttggaggt gaactagtta caattgccca tttggatcgg 1680

cctttgtatg tcttaggata tgtgcctaaa gatgatagat tatacctggt agataaagag 1740

ttgcgcgtag taagctacca attactcctc tctgttcttg aatatcaaac tgccgtcatg 1800

agaagagact ttccaacagc agatagagta cttccgtcta ttcctaagga gcacagaacg 1860

agagtcgcac atttcttaga aaagcaaggc ttcaaacagc aagctttggc cgtaagtaca 1920

gatccagagc acagattcga gttggcagta gcattagagg atcttaacac agccaagatt 1980

ctagctcaag aagcaaacag tccgcaaaag tggaatcaac tagcagaatt ggctgccgct 2040

actaataacg taagcctagc caaggaatgt atgcagaaag cgcaagatta tggtggctta 2100

ttgcttcttg ctacgagttc cggagatgaa agtttagtcc gtactctagg agaaacgaca 2160

caagcagaaa gcaaacataa cttagcattt ttgtcacact tgttagtagg tgatttaaac 2220

aaatgtctag acatccttat taataccggt agattgccag aagctgcatt tttcgccaga 2280

tcttatcttc ctgataagat aacggaagtc gtagaacttt ggaagactca gttatcttca 2340

gtcaatcaaa aagctggaca gagccttgcc gatccgaaaa attacgaaaa tctgttccct 2400

ggtttacaag aggcagtggt agcccagaaa tttttggaac agcagaataa aggtttagca 2460

cccgcgcgaa ttgccaccac tattcctcct aatcacgaca ggaatgttgt agccgaagtt 2520

caagcacaat cgaaacatga tggaccatca tttagttcga ggagtctttc atcagaaata 2580

gaagcacaaa caaggagttc tgctaaacct gaagaatctt caaacattat ccagttggat 2640

caagatgatg acgatatcga tttagatttg gacggtgtaa atatcgatga gaacattgac 2700

c 2701

<210>878

<211>2714

<212>DNA

<213>Diabrotica virgifera zeae

<400>878

atgccacttc gattagatat aaaaagaaag ctaacagctc gctcagaccg ggtaaaatgt 60

gtggatcttc accctacaga accttggatg ctatgttctc tttacagcgg aaatataaac 120

gtttggaaca ccgaaaatca gcaactggtt aagacttttg aagtatgtga tgtacctgtt 180

cggacagcta agtttttgcc caggaagaac tggatagtca gtgggtctga tgatatgcag 240

attcgagttt tcaattacaa taccttagat cgggtacatt cttttgaggc tcattcggat 300

tatgtgagat gtattgtcgt acaccctaca caaccttata tattaacaag tagtgatgat 360

atgcttatca agctttggaa ttgggaaaaa gcatgggctt gtcagcaagt tttcgaagga 420

cacactcatt atattatgca aattgccata aatccaaaag acaacaacac atttgccagt 480

gcatccctag atagaacatt gaaagtatgg caattgggag cgtccacagc gaatttcaca 540

ctagaaggtc atgagaaagg cgttaactgt gtggactatt atcacggtgg agataaacct 600

tatttaatct caggcgctga tgatagatta gtaaaaatct gggattatca aaacaaaact 660

tgtgttcaaa ctttggaagg acatgctcaa aatgtaaccg ctgcatgttt ccatccagaa 720

cttcctgtag ctcttactgg aagtgaagat ggtactgtca gagtgtggca tgccaacacc 780

cataggttag aaagtagctt aaattatggc tttgaaagag tatggactat tttctgccta 840

aagggatcca ataacgtggc attgggttat gatgaaggta gcattttggt taaagttggt 900

agagaagaac cagctgttag tatggatgcc agtggaggca aaattatttg ggccagacac 960

tctgaacttc aacaggcaaa tctcaaggcg ttagctgaag gtgcggaaat aagagatgga 1020

gaacgccttc cagtttctgt aaaagatatg ggtgcttgcg agatataccc tcagacaatt 1080

caacacaatc ccaatggccg ttttgttgtt gtctgtgggg atggagaata cataatctac 1140

acagcaatgg ctttaagaaa caaagcgttt ggtagcgcac aagaatttgt gtgggctcaa 1200

gattccagcg aatatgccat cagagaatcc ggatctacta tcagaatttt taagaatttc 1260

aaagagaaga agaattttaa gcccgatttt ggagctgaag gtatatatgg tggatacctt 1320

ttgggagtca aatcggtttc tggtttgact ttctatgatt gggaaactct tgatttagtc 1380

agaagaatcg agatacaacc aaaagcagtt tactggtcag atagtggtaa attagtatgt 1440

ttggccacag aagatagcta ctttattctt tcttatgatt ctgatgaagt tcaaaaagcc 1500

agagataaca atcaggttgc ggatgatgga gtagaatcgg ctttcaatct tctaggtgaa 1560

ataaacgaat cagtgcgaac tggtctctgg gtaggcgact gttttatcta cacgaattct 1620

gttaatcgta tcaactactt cgttggaggt gaactggtta caattgctca tttggaccgg 1680

cctttgtatg tcttgggata tgtgcctaaa gacgatagat tatacctcgt agataaagag 1740

ttgcgcgtag taagctacca attacttctt tctgttcttg aatatcaaac tgccgtcatg 1800

agaagagact ttccaacagc agacagagta cttccgtcca ttcctaagga gcacagaacg 1860

agagtggcac atttcttaga aaagcaaggc ttcaaacagc aagctttggc cgtaagtaca 1920

gatccagagc acagattcga gctggcagta gcattagagg atcttaatat agccaaaact 1980

ctagctcaag aagcgaacag tccgcaaaag tggaatcaac tagcagaatt ggcagctgct 2040

actaataatg taagcttagc caaggaatgt atgcaaaaag cgcaagatta tggaggcttg 2100

ttgcttcttg ctacgagctc cggtgatgaa aatttagtcc gtgctctagg agaaacgaca 2160

caagctgaaa gcaaacataa cttagcattt ttgtcacact tgttagtagg tgatttaaac 2220

aaatgtctag acattcttat taataccggt agattgccag aagctgcatt tttcgccaga 2280

tcttaccttc ctgataagat tacagaagtc gtggaactgt ggaagactca gttatcttca 2340

gtcaatcaaa aagctggaca gagccttgcc gatcctaaaa actacgaaaa tctgttccct 2400

ggtttacaag aggcggtggt agctcagaaa tttttggaac agcagaataa aggtttagcg 2460

cccgcaagag ttgccaccac cattcctcct aatcacgaca ggaatgttgt agccgaagtt 2520

caagcacaat cgaaacacga tgtaccatca tttagttctt cgtttatttc atcagaaata 2580

gaagcacaaa caaggagttc tgctaaacct gaagaatctt caaacattat acagctggac 2640

caagatgacg acgatatcga tttagatttg gacggtgtaa atatcgatga gaacattgac 2700

acgacggata tcaa 2714

<210>879

<211>2714

<212>DNA

<213>Diabrotica virgifera virgifera

<400>879

atgccacttc gattagatat aaaaagaaag ctaacagctc gctcagaccg ggtaaaatgt 60

gtggatcttc accctacaga accttggatg ctgtgttctc tttacagcgg aaatataaac 120

gtttggaaca ccgaaaatca gcaactggtt aagacttttg aagtatgtga tgtacctgtt 180

cggacagcta agtttttgcc caggaagaac tggatagtca gtgggtctga tgatatgcag 240

attcgagttt tcaattacaa taccttagat cgggtacatt cttttgaggc tcattcggat 300

tatgtgagat gtattgtcgt acaccctaca caaccttata tattaacaag tagtgatgat 360

atgcttatca agctttggaa ttgggaaaaa gcatgggctt gtcagcaagt tttcgaagga 420

cacactcatt atattatgca aatcgccata aatccaaaag acaacaacac atttgccagt 480

gcatccctag atagaacatt gaaagtatgg caattgggag cgtccacagc gaatttcaca 540

ctagaaggtc atgagaaagg cgttaactgt gtggactatt atcacggtgg agataaacct 600

tatttaatct caggcgctga tgatagatta gtaaaaatct gggattatca aaacaaaact 660

tgtgttcaaa ctttggaagg acatgctcaa aatgtaaccg ctgcatgttt ccatccagaa 720

cttcctgtag ctcttactgg aagtgaagat ggtactgtca gagtgtggca tgccaacacc 780

cataggttag aaagtagctt aaattatggc tttgaaagag tatggactat tttctgccta 840

aagggatcca ataacgtggc attgggttat gatgaaggta gcattttggt taaagttggt 900

agagaagaac cagctgttag tatggatgcc agtggaggca aaattatttg ggccagacac 960

tctgaacttc aacaggcaaa tctcaaggcg ttagctgaag gtgcggaaat aagagatgga 1020

gaacgccttc cagtttctgt aaaagatatg ggtgcttgcg agatataccc tcagacaatt 1080

caacacaatc ccaatggccg ttttgttgtt gtctgtgggg atggagaata cataatctac 1140

acagcaatgg ctttaagaaa caaagcgttt ggtagcgcac aagaatttgt gtgggctcaa 1200

gattccagcg aatatgccat cagagaatcc ggatctacta tcagaatttt taagaatttc 1260

aaagagaaga agaattttaa gtccgatttt ggagctgaag gtatatacgg tggatacctt 1320

ttgggagtca aatcggtttc tggtttgact ttctatgatt gggaaactct cgatttagtc 1380

agaagaatcg agatacaacc aaaagcagtt tactggtcag atagtggtaa attagtatgt 1440

ttggccacag aagatagcta ctttattctt tcttatgatt ctgatgaagt tcaaaaagcc 1500

agagataaca atcaggttgc ggatgatgga gtagaatcgg ctttcaatct tctaggtgaa 1560

ataaacgaat cagtgcgaac tggtctctgg gtaggcgact gttttatcta cacgaattct 1620

gttaatcgta tcaactactt cgttggaggt gaactggtta caattgctca tttggaccgg 1680

cctttgtatg tcttgggata tgtgcctaaa gacgatagat tatacctcgt agataaagag 1740

ttgcgcgtag taagctacca attacttctt tctgttcttg aatatcaaac tgccgtcatg 1800

agaagagact ttccaacagc agacagagta cttccgtcca ttcctaagga gcacagaacg 1860

agagtggcac atttcttaga aaagcaaggc ttcaaacagc aagctttggc cgtaagtaca 1920

gatccagagc acagattcga gctggcagta gcattagagg atcttaatat agccaaaact 1980

ctagctcaag aagcgaacag tccgcaaaag tggaatcaac tagcagaatt ggcagctgct 2040

actaataatg taagcgtagc caaggaatgt atgcaaaaag cgcaagatta tggaggcttg 2100

ttgcttcttg ctacgagctc cggtgatgaa aatttagtcc gtactctagg agaaacgaca 2160

caagctgaaa gcaaacataa cttagcattt ttgtcacact tgttagtagg tgatttaaac 2220

aaatgtctag acattcttat taataccggt agattgccag aagctgcatt tttcgccaga 2280

tcttaccttc ctgataagat tacagaagtc gtggaactgt ggaagactca gttatcttca 2340

gtcaatcaaa aagctggaca gagccttgcc gatcctaaaa actacgaaaa tctgttccct 2400

ggtttacaag aggcggtggt agctcagaaa tttttggaac agcagaataa aggtttagcg 2460

cccgcaagag ttgccaccac cattcctcct aatcacgaca ggaatgttgt agccgaagtt 2520

caagcacaat cgaaacacga tgtaccatca tttagttctt cgtttatttc atcagaaata 2580

gaagcacaaa caaggagttc tgctaaacct gaagaatctt caaacattat acagctggac 2640

caagatgacg acgatatcga tttagatttg gacggtgtaa atatcgatga gaacattgac 2700

acgacggata tcaa 2714

<210>880

<211>669

<212>DNA

<213>Epilachna varivestis

<400>880

cgagggccac acccaytatg ttatgcaaat tgccataaat cctaaggata ataatacttt 60

tgctagtgca tcattagata gaacactcaa agtttggcag ttaggtgctt ccacagcaaa 120

ttttacttta gaaggtcacg ataaaggagt aaactgcgtt gattattatc atggtggaga 180

taaaccttat cttatatctg gagctgatga tcgattggta aaaatatggg attatcaaaa 240

caagacatgt gttcaaactt tagaaggtca tgcccaaaat gtaactgcag tttgcttcca 300

tccagaattg ccagtagttt taacaggtag tgaagatgga actgttagga tttggcatgc 360

caatactcat aggctggaaa gcagtttgaa ttatggattc gagagagtat gggccatttc 420

ttgcttaaag gggtccaaca atgtggttct gggttatgat gaaggtagtg tcctagttaa 480

agttggtcgt gaagaaccag ctgtcagtat ggatgctagt ggtggaaaaa ttatttgggc 540

ccgccattcc gaattgcaac aggctaattt gaaggcgctt cctgaagggg cggaggttag 600

agatggcgaa cgtttacctg ttgctgttaa agacatgggc gcttgygaaa tttaccccca 660

gaccatcgc 669

<210>881

<211>1033

<212>DNA

<213>Leptinotarsa decemlineata

<400>881

ccatggtggc gataaaccat acttgatatc gggagcagac gatcggttgg ttaaaatctg 60

ggactatcaa aacaaaacgt gtgtccaaac cttggaagga cacgcccaaa acgtaaccgc 120

ggtttgtttc caccctgaac tacctgtggc tctcacaggc agcgaagatg gtaccgttag 180

agtttggcat acgaatacac acagattaga gaattgtttg aattatgggt tcgagagagt 240

gtggaccatt tgttgcttga agggttcgaa taatgtttct ctggggtatg acgagggcag 300

tatattagtg aaagttggaa gagaagaacc ggcagttagt atggatgcca gtggcggtaa 360

aataatttgg gcaaggcact cggaattaca acaagctaat ttgaaggcgc tgccagaagg 420

tggagaaata agagatgggg agcgtttacc tgtctctgta aaagatatgg gagcatgtga 480

aatataccct caaacaatcc aacataatcc gaatggaaga ttcgttgtag tatgcggaga 540

cggcgaatat atcatttaca cagcgatggc tctacggaac aaggcttttg gaagcgctca 600

agagtttgtc tgggctcagg actccagcga gtatgccatt cgcgagtctg gttccacaat 660

tcggatattc aaaaacttca aagaaaggaa gaacttcaag tcggatttca gcgcggaagg 720

aatctacggg ggttttctct tggggattaa atcggtgtcc ggtttaacgt tttacgattg 780

ggaaactttg gacttggtga gacggattga aatacaaccg agggcggttt attggtctga 840

cagtggaaaa ttagtctgtc tcgcaacgga ggacagctac ttcatccttt cttatgattc 900

ggagcaagtt cagaaggcca gggagaacaa tcaagtcgca gaggatggcg tagaggccgc 960

tttcgatgtg ttgggggaaa tgaacgagtc tgtccgaaca ggtctttggg tcggagactg 1020

tttcatctat acc 1033

<210>882

<211>601

<212>DNA

<213>Diabrotica balteata

<400>882

gtcctgggga ggctattcaa aaactcagag agactgaaga aatgttaata aaaaaacagg 60

attttttaga aaagaagata gaagaatata ccttagtagc aaagaaaaat gcgtcgaaaa 120

ataaaagagt tgcactccaa gctctcaaaa agaagaaacg attggaaaag acccaactac 180

aaatagatgg aacccttaca actattgaaa tgcagaggga agccctcgaa ggagctagca 240

caaatactgc tgtattagat tctatgaaaa atgctgcaga tgcccttaag aaagctcata 300

agaacttgaa tgtggatgat gttcacgaca tcatggatga catagccgaa caacacgaca 360

tagccaacga aatcacaaac gctattagca accctgttgg attcaccgac gatctggacg 420

acgatgaatt agagaaagaa ttagaagagc tcgaacaaga aggattggaa gaagacctgc 480

ttcaagtgcc aggtcccact caactgccgg ctgtgcctac tgatgcagtt gctaataaac 540

caatcaaacc agcagctaga aaagttgaag atgatgacga tatgaaagaa ttggaagcct 600

g 601

<210>883

<211>601

<212>DNA

<213>Diabrotica barberi

<400>883

gtcctgggga ggctattcaa aaactcagag agactgaaga aatgttaata aaaaaacagg 60

attttttaga aaagaagata gaagaattta ccatggtagc aaagaaaaat gcgtcgaaaa 120

ataaaagagt tgcactccaa gccctcaaaa agaagaaacg attggaaaag actcaactac 180

aaatagatgg aacccttaca actattgaaa tgcagaggga agccctcgaa ggagctagca 240

caaatactgc tgtattagat tctatgaaaa atgctgcaga tgcccttaag aaagctcata 300

agaatttgaa tgtagatgat gttcacgata tcatggatga catagccgaa caacacgaca 360

tagccaacga aattacaaat gctattagca accctgttgg attcaccgac gatctggacg 420

acgatgaatt agaaaaagaa ttagaagagc tcgaacaaga aggattggaa gaagacctgc 480

tccaagtgcc aggtccaact caactgccgg ctgtgcctgc tgatgcagtt actactaaac 540

caatcaaacc agcagctaaa aaagttgaag atgatgacga tatgaaagaa ttggaagcct 600

g 601

<210>884

<211>601

<212>DNA

<213>Diabrotica undecimpunctata howardii

<400>884

gtcctgggga ggctattcaa aaactcagag agactgaaga aatgttaata aaaaaacagg 60

attttttaga aaagaagata gaagaattta ccttagtagc aaagaaaaat gcgtcgaaaa 120

ataaaagagt tgcactccaa gccctcaaaa agaagaaacg attggaaaag acccaactac 180

aaatagatgg aacccttaca actattgaaa tgcagaggga agccctcgaa ggagctagca 240

caaatactgc tgtattagat tctatgaaaa atgctgcaga tgcccttaag aaagctcata 300

agaacttgaa tgtggatgat gttcacgaca tcatggatga catagccgaa caacacgaca 360

tagccaacga aatcacaaac gctattagca atcctgttgg attcaccgac gatctggacg 420

atgatgaatt agagaaagaa ttagaagagc tcgaacaaga aggattggaa gaagacctgc 480

ttcaagtgcc aggtccaact caactgccgg ctgtgcctac tgatgcagtt gctaataaac 540

caattaaacc agcagctaga aaagttgaag atgatgacga tatgaaagaa ttggaagcct 600

g 601

<210>885

<211>601

<212>DNA

<213>Diabrotica virgi fera zeae

<400>885

gtcctgggga ggctattcaa aaactcagag agactgaaga aatgttaata aaaaaacagg 60

attttttaga aaagaagata gaagaattta ccatggtagc aaagaaaaat gcgtcgaaaa 120

ataaaagagt tgcactccaa gccctcaaaa agaagaaacg attggaaaag acccaactac 180

aaatagatgg aacccttaca actattgaaa tgcagaggga agccctcgaa ggagctagca 240

caaatactgc tgtattagat tctatgaaaa atgctgcaga tgcccttaag aaagctcata 300

agaatttgaa tgtagatgat gttcacgata tcatggatga catagccgaa caacacgaca 360

tagccaacga aatcacaaac gctattagca atcctgtcgg attcaccgac gatctggatg 420

acgatgaatt agaaaaagaa ttagaagagc tcgaacaaga aggattggaa gaagacctgc 480

tccaagtgcc aggtccaact caactgccgg ctgtgcctgc tgatgcagtt gctactaaac 540

caatcaaacc agcagctaaa aaagttgaag atgatgacga tatgaaagaa ttggaagcct 600

g 601

<210>886

<211>601

<212>DNA

<213>Diabrotica virgifera virgifera

<400>886

gtcctgggga ggctattcaa aaactcagag agactgaaga aatgttaata aaaaaacagg 60

attttttaga aaagaagata gaagaattta ccatggtagc aaagaaaaat gcgtcgaaaa 120

ataaaagagt tgcactccaa gccctcaaaa agaagaaacg attggaaaag acccaactac 180

aaatagatgg aacccttaca actattgaaa tgcagaggga agccctcgaa ggagctagca 240

caaatactgc tgtattagat tctatgaaaa atgctgcaga tgcccttaag aaagctcata 300

agaatttgaa tgtagatgat gttcacgata tcatggatga catagccgaa caacacgaca 360

tagccaacga aatcacaaac gctattagca atcctgtcgg attcaccgac gatctggatg 420

acgatgaatt agaaaaagaa ttagaagagc tcgaacaaga aggattggaa gaagacctgc 480

tccaagtgcc aggtccaact caactgccgg ctgtgcctgc tgatgcagtt gctactaaac 540

caatcaaacc agcagctaaa aaagttgaag atgatgacga tatgaaagaa ttggaagcct 600

g 601

<210>887

<211>145

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.1 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv23-Dv13 order is formed

<400>887

cctcaagctg tcattggcat ggatattctg tgccaagcta aatccggtat gggaaaaacg 60

gctgtttttg caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc 120

aaatttcttc ctccagtgat catac 145

<210>888

<211>222

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.2 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of 23-Dv13-Dv26 order is formed

<400>888

cctcaagctg tcattggcat ggatattctg tgccaagcta aatccggtat gggaaaaacg 60

gctgtttttg caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc 120

aaatttcttc ctccagtgat catacccata cgatttacct gaggaaatgc cggataatct 180

gaagcaacat ataaggggta tcaacaatac tagagagttg ag 222

<210>889

<211>275

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.3 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv23-Dv13-Dv26-Dv18 order is formed

<400>889

cctcaagctg tcattggcat ggatattctg tgccaagcta aatccggtat gggaaaaacg 60

gctgtttttg caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc 120

aaatttcttc ctccagtgat catacccata cgatttacct gaggaaatgc cggataatct 180

gaagcaacat ataaggggta tcaacaatac tagagagttg agtcaaacat aattatggca 240

cattgtcctt ggatgtagta tccatcacgc agacc 275

<210>890

<211>338

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.4 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv23-Dv13-Dv26-Dv18-Dv49 order is formed

<400>890

cctcaagctg tcattggcat ggatattctg tgccaagcta aatccggtat gggaaaaacg 60

gctgtttttg caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc 120

aaatttcttc ctccagtgat catacccata cgatttacct gaggaaatgc cggataatct 180

gaagcaacat ataaggggta tcaacaatac tagagagttg agtcaaacat aattatggca 240

cattgtcctt ggatgtagta tccatcacgc agaccgccga acaacacgac atagccaacg 300

aaatcacaaa cgctattagc aatcctgtcg gattcacc 338

<210>891

<211>418

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.5 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv23-Dv1 3-Dv26-Dv18-Dv49-Dv22 order is formed

<400>891

cctcaagctg tcattggcat ggatattctg tgccaagcta aatccggtat gggaaaaacg 60

gctgtttttg caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc 120

aaatttcttc ctccagtgat catacccata cgatttacct gaggaaatgc cggataatct 180

gaagcaacat ataaggggta tcaacaatac tagagagttg agtcaaacat aattatggca 240

cattgtcctt ggatgtagta tccatcacgc agaccgccga acaacacgac atagccaacg 300

aaatcacaaa cgctattagc aatcctgtcg gattcaccgt aatgcactcc tacgtcgttt 360

ggttcgtatt ggagtactag acgagaacag aatgaagctc gattacgtat tgggtctc 418

<210>892

<211>152

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.6 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv13-Dv26 order is formed

<400>892

caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc aaatttcttc 60

ctccagtgat catacccata cgatttacct gaggaaatgc cggataatct gaagcaacat 120

ataaggggta tcaacaatac tagagagttg ag 152

<210>893

<211>205

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.7 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv13-Dv26-Dv18 order is formed

<400>893

caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc aaatttcttc 60

ctccagtgat catacccata cgatttacct gaggaaatgc cggataatct gaagcaacat 120

ataaggggta tcaacaatac tagagagttg agtcaaacat aattatggca cattgtcctt 180

ggatgtagta tccatcacgc agacc 205

<210>894

<211>268

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.8 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv13-Dv26-Dv18-Dv49 order is formed

<400>894

caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc aaatttcttc 60

ctccagtgat catacccata cgatttacct gaggaaatgc cggataatct gaagcaacat 120

ataaggggta tcaacaatac tagagagttg agtcaaacat aattatggca cattgtcctt 180

ggatgtagta tccatcacgc agaccgccga acaacacgac atagccaacg aaatcacaaa 240

cgctattagc aatcctgtcg gattcacc 268

<210>895

<211>348

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.9 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv13-Dv26-Dv18-Dv49-Dv22 order is formed

<400>895

caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc aaatttcttc 60

ctccagtgat catacccata cgatttacct gaggaaatgc cggataatct gaagcaacat 120

ataaggggta tcaacaatac tagagagttg agtcaaacat aattatggca cattgtcctt 180

ggatgtagta tccatcacgc agaccgccga acaacacgac atagccaacg aaatcacaaa 240

cgctattagc aatcctgtcg gattcaccgt aatgcactcc tacgtcgttt ggttcgtatt 300

ggagtactag acgagaacag aatgaagctc gattacgtat tgggtctc 348

<210>896

<211>408

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.10 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv13-Dv26-Dv18-Dv49-Dv22-Dv20 order is formed

<400>896

caaaggatcc aggatgacgt tccctattga taactgtacc tacacgaccc aaatttcttc 60

ctccagtgat catacccata cgatttacct gaggaaatgc cggataatct gaagcaacat 120

ataaggggta tcaacaatac tagagagttg agtcaaacat aattatggca cattgtcctt 180

ggatgtagta tccatcacgc agaccgccga acaacacgac atagccaacg aaatcacaaa 240

cgctattagc aatcctgtcg gattcaccgt aatgcactcc tacgtcgttt ggttcgtatt 300

ggagtactag acgagaacag aatgaagctc gattacgtat tgggtctccg aagtaatgta 360

caacggccac actggacgta agataaatgc tcagattttc ttaggacc 408

<210>897

<211>130

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.11 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv26-Dv18 order is formed

<400>897

ccatacgatt tacctgagga aatgccggat aatctgaagc aacatataag gggtatcaac 60

aatactagag agttgagtca aacataatta tggcacattg tccttggatg tagtatccat 120

cacgcagacc 130

<210>898

<211>193

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.12 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv26-Dv18-Dv49 order is formed

<400>898

ccatacgatt tacctgagga aatgccggat aatctgaagc aacatataag gggtatcaac 60

aatactagag agttgagtca aacataatta tggcacattg tccttggatg tagtatccat 120

cacgcagacc gccgaacaac acgacatagc caacgaaatc acaaacgcta ttagcaatcc 180

tgtcggattc acc 193

<210>899

<211>273

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.13 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv26-Dv18-Dv49-Dv22 order is formed

<400>899

ccatacgatt tacctgagga aatgccggat aatctgaagc aacatataag gggtatcaac 60

aatactagag agttgagtca aacataatta tggcacattg tccttggatg tagtatccat 120

cacgcagacc gccgaacaac acgacatagc caacgaaatc acaaacgcta ttagcaatcc 180

tgtcggattc accgtaatgc actcctacgt cgtttggttc gtattggagt actagacgag 240

aacagaatga agctcgatta cgtattgggt ctc 273

<210>900

<211>333

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.14 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv26-Dv18-Dv49-Dv22-Dv20 order is formed

<400>900

ccatacgatt tacctgagga aatgccggat aatctgaagc aacatataag gggtatcaac 60

aatactagag agttgagtca aacataatta tggcacattg tccttggatg tagtatccat 120

cacgcagacc gccgaacaac acgacatagc caacgaaatc acaaacgcta ttagcaatcc 180

tgtcggattc accgtaatgc actcctacgt cgtttggttc gtattggagt actagacgag 240

aacagaatga agctcgatta cgtattgggt ctccgaagta atgtacaacg gccacactgg 300

acgtaagata aatgctcaga ttttcttagg acc 333

<210>901

<211>116

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.15 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv18-Dv49 order is formed

<400>901

tcaaacataa ttatggcaca ttgtccttgg atgtagtatc catcacgcag accgccgaac 60

aacacgacat agccaacgaa atcacaaacg ctattagcaa tcctgtcgga ttcacc 116

<210>902

<211>196

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.16 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv18-Dv49-Dv22 order is formed

<400>902

tcaaacataa ttatggcaca ttgtccttgg atgtagtatc catcacgcag accgccgaac 60

aacacgacat agccaacgaa atcacaaacg ctattagcaa tcctgtcgga ttcaccgtaa 120

tgcactccta cgtcgtttgg ttcgtattgg agtactagac gagaacagaa tgaagctcga 180

ttacgtattg ggtctc 196

<210> 903

<211>256

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.17 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv18-Dv49-Dv22-Dv20 order is formed

<400>903

tcaaacataa ttatggcaca ttgtccttgg atgtagtatc catcacgcag accgccgaac 60

aacacgacat agccaacgaa atcacaaacg ctattagcaa tcctgtcgga ttcaccgtaa 120

tgcactccta cgtcgtttgg ttcgtattgg agtactagac gagaacagaa tgaagctcga 180

ttacgtattg ggtctccgaa gtaatgtaca acggccacac tggacgtaag ataaatgctc 240

agattttctt aggacc 256

<210>904

<211>143

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.18 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv49-Dv22 order is formed

<400>904

gccgaacaac acgacatagc caacgaaatc acaaacgcta ttagcaatcc tgtcggattc 60

accgtaatgc actcctacgt cgtttggttc gtattggagt actagacgag aacagaatga 120

agctcgatta cgtattgggt ctc 143

<210>905

<211>203

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.19 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv49-Dv22-Dv20 order is formed

<400>905

gccgaacaac acgacatagc caacgaaatc acaaacgcta ttagcaatcc tgtcggattc 60

accgtaatgc actcctacgt cgtttggttc gtattggagt actagacgag aacagaatga 120

agctcgatta cgtattgggt ctccgaagta atgtacaacg gccacactgg acgtaagata 180

aatgctcaga ttttcttagg acc 203

<210>906

<211>140

<212>DNA

＜213〉artificial sequence

<220>

＜223〉artificial sequence note: the C12.20 concatermer of highly effective WCR target, about 50%GC standard is by 5 '-3 '

For the fragment of Dv22-Dv20 order is formed

<400>906

gtaatgcact cctacgtcgt ttggttcgta ttggagtact agacgagaac agaatgaagc 60

tcgattacgt attgggtctc cgaagtaatg tacaacggcc acactggacg taagataaat 120

gctcagattt tcttaggacc 140

Claims (34)

1. isolating polynucleotide are selected from down group:

(a) comprise the polynucleotide of the nucleotide sequence of SEQ ID NO:1-SEQ ID NO:906;

(b) at 5X SSC, under 50% methane amide and 42 ℃ of following 10 minutes the wash conditions with the polynucleotide of the nucleic acid array hybridizing of SEQ IDNO:1-SEQ ID NO:906;

(c) comprise polynucleotide with at least 70% sequence identity of the nucleotide sequence of SEQ ID NO:1-SEQ ID NO:906;

(d) fragment of at least 21 adjacent nucleotides of the nucleotide sequence of SEQ ID NO:1-SEQ ID NO:906, wherein the Coleoptera plant insect is taken in the double-stranded ribonucleoside acid sequence comprise with at least one chain of described fragment complementation, suppresses the growth of described insect; With

(e) complementary sequence of above-mentioned (a) and (b), (c) or sequence (d).

2. the isolating polynucleotide of claim 1 are selected from SEQ ID NO:697, SEQ IDNOs:813-819, SEQ ID NO:841 and SEQ ID NO:874.

3. the isolating polynucleotide of claim 1, it is operably connected with allogeneic promoter.

4. the isolating polynucleotide of claim 1, it is included on the plant conversion carrier.

5. the double-stranded ribonucleoside acid sequence that is produced by the expression of the polynucleotide of claim 1, wherein the Coleoptera plant insect is taken in described ribonucleoside acid sequence, suppresses the growth of described insect.

6. the double-stranded ribonucleoside acid sequence of claim 5, comprise first by preparation, the recombination of polynucleotide sequence of the second and the 3rd polynucleotide sequence produces, wherein said first polynucleotide sequence comprises the isolating polynucleotide of claim 1, wherein the 3rd polynucleotide sequence is connected with first polynucleotide sequence by second polynucleotide sequence, and wherein the 3rd polynucleotide sequence is the reverse complementary sequence of first polynucleotide sequence substantially, make when being transcribed into Yeast Nucleic Acid, the hybridization of the described first and the 3rd polynucleotide sequence forms the double-stranded ribonucleoside acid sequence by the second ribonucleotide sequence stabilization that connects.

7. the double-stranded ribonucleoside acid sequence of claim 5, wherein insect is taken in polynucleotide sequence, suppresses the expression with the basic complementary nucleotide sequence of described polynucleotide sequence.

8. use the polynucleotide cell transformed of claim 1.

9. the cell of claim 8, it is a prokaryotic cell prokaryocyte.

10. the cell of claim 8, it is an eukaryotic cell.

11. the cell of claim 8, it is plant or bacterial cell.

12. polynucleotide plant transformed with claim 1.

13. the seed of the plant of claim 12, wherein said seed comprises polynucleotide.

14. polynucleotide plant transformed with claim 2.

15. the plant of claim 12, wherein said polynucleotide are expressed as double-stranded ribonucleoside acid sequence in vegetable cell, and, take in the described double-stranded ribonucleoside acid sequence be present in the insect pest amount of suppression in the food, suppress the insect described food of further ingesting.

16. the plant of claim 15, wherein said insect pest are selected from Diabrotica virgifera, Diabrotica virgifera virgifera, Diabrotica virgifera zea, Diabroticabalteata, Diabrotica barberi, Diabrotica viridula, Diabrotica speciosa and Diabrotica undecimpunctata.

17. the plant of claim 15 is wherein taken in the double-stranded ribonucleoside acid sequence of insect pest amount of suppression, and the insect growth is stopped.

18. Accessory Right requires the agricultural-food goods of 12 plant production, but wherein said agricultural-food goods comprise detection limit claim 1 polynucleotide or by the ribonucleotide of its expression.

19. the method for control coleopteran pest invasion and attack, be included in the food of coleopteran pest a kind of reagent is provided, this reagent comprises first polynucleotide sequence, described polynucleotide sequence works after insect is taken in, suppress the biological function in the described insect, wherein said polynucleotide sequence is along the nucleotide sequence homology that has about 95%-about 100% at least about about 25 adjacent nucleotides of 19-and the encoding sequence that derives from described insect, and hybridize in the described first polynucleotide sequence complementary, second polynucleotide sequence, and the described encoding sequence that wherein derives from described insect is selected from SEQ ID NO:1-SEQ ID NO:906 and complementary sequence thereof.

20. the method for claim 19, wherein said coleopteran pest is chrysomelid species, is selected from Diabrotica virgifera, Diabrotica virgifera virgifera, Diabrotica virgiferazea, Diabrotica balteata, Diabrotica barberi, Diabrotica viridula, Diabrotica speciosa and Diabrotica undecimpunctata.

21. the method for control coleopteran pest invasion and attack, be included in the vegetable cell that the polynucleotide sequence of expressing claim 1 is provided in the food of coleopteran pest, wherein express described polynucleotide, produce double-stranded ribonucleotide, it works after insect is taken in, suppress target sequence and express in described insect, and cause with respect to the food that lacks described vegetable cell, insect is to the minimizing of ingesting of described food.

22. the growth after taking in cell of the method for claim 21, wherein said insect reduces.

23. the method for claim 21, wherein said vegetable cell further comprise the polynucleotide sequence that coding is selected from down the sterilant of group: potato tuber storage protein, bacillus thuringiensis insecticidal albumen, Xenorhabdus insecticidal protein, polished rod bacterium insecticidal protein, bacillus laterosporus insecticidal protein and Bacillus sphaericus insecticidal protein.

24. the method for claim 23, wherein said bacillus thuringiensis insecticidal albumen is selected from: the combination of Cry1, Cry3, TIC851, CryET70, Cry22, TIC901, TIC1201, TIC407, TIC417, binary insecticidal protein CryET33 and CryET34, binary insecticidal protein CryET80 and CryET76, binary insecticidal protein TIC100 and TIC101, insecticidal protein ET29 or ET37 and insecticidal protein TIC810 or TIC812, and binary insecticidal protein PS149B1.

25. the method for claim 21, wherein said target sequence proteins encoded, described proteic forecast function is selected from: muscle forms, the formation of neotonin, the adjusting of neotonin, ion is regulated and transhipment, digestive ferment is synthetic, cell membrane potential keeps, amino acid bio is synthetic, amino acid degradation, sperm forms, pheromone is synthetic, the pheromone sensing, feeler forms, wing forms, leg forms, grow and differentiation, avette one-tenth, the larva maturation, digestive ferment forms, hemolymph is synthetic, the maintenance of hemolymph, neurotransmission, cell fission, energy metabolism, breathe, and apoptosis.

26. the method for claim 21, wherein said coleopteran pest is chrysomelid species, is selected from Diabrotica virgifera, Diabrotica virgifera virgifera, Diabrotica virgiferazea, Diabrotica balteata, Diabrotica barberi, Diabrotica viridula, Diabrotica speciosa and Diabrotica undecimpunctata.

27. the method for claim 21, described polynucleotide worked after wherein insect was taken in, prevent and exercise the survive gene of necessary function of insect, described function is selected from: insect ingests, the insect apoptosis, cytodifferentiation and growth, sexual propagation ability or needs, muscle forms, muscle twitch, Muscle contraction, the formation of neotonin, the adjusting of neotonin, ion is regulated and transhipment, the maintenance of cell membrane potential, amino acid bio is synthetic, amino acid degradation, sperm forms, pheromone is synthetic, the pheromone sensing, feeler forms, wing forms, leg forms, avette one-tenth, the larva maturation, digestive ferment forms, hemolymph is synthetic, the maintenance of hemolymph, neurotransmission, larval stage changes, pupate, broken pupa, cell fission, energy metabolism, the formation of breathing and cytoskeletal structure.

28. the method for the crop yield that the crop plants of improvement insect pest invasion and attack produces said method comprising the steps of:

A) polynucleotide of claim 1 are introduced described crop plants,

B) cultivate described crop plants, described polynucleotide are expressed, the expression inhibiting insect pest of wherein said polynucleotide is ingested and because the production loss that the insect invasion and attack cause.

29. the method for claim 28, the expression of wherein said polynucleotide produces the RNA molecule, described RNA molecule is prevented first target gene in the insect pest of the part of taking in described crop plants at least, wherein said target gene is exercised at least a household function that is selected from down group: insect ingests, the insect viability, the insect apoptosis, the differentiation of insect or any insect cell and growth, the sexual propagation of insect, muscle forms, muscle twitch, Muscle contraction, the formation of neotonin and/or minimizing, the adjusting of neotonin, ion is regulated and transhipment, the maintenance of cell membrane potential, amino acid bio is synthetic, amino acid degradation, sperm forms, pheromone is synthetic, the pheromone sensing, feeler forms, wing forms, leg forms, avette one-tenth, the larva maturation, digestive ferment forms, hemolymph is synthetic, hemolymph keeps, neurotransmission, larval stage changes, pupate, broken pupa, cell fission, energy metabolism, breathe, synthetic and the maintenance of cytoskeletal structure, nucleotide metabolism, nitrogen metabolism, water conservancy is used, water keeps, and sensory perception.

30. the method for claim 28, wherein said insect pest are the corn rootworm insect that is selected from down group: Diabrotica undecimpunctata howardi (southern corn rootworm (SCR)), Diabrotica virgifera virgifera (western corn rootworm (WCR)), Diabrotica barberi (northern corn rootworm (NCR)), Diabrotica virgifera zea (zea mexicana rootworm (MCR)), Diabrotica balteata (Brazilian corn rootworm (BZR)), Diabrotica viridula (Brazilian corn rootworm (BZR)) and Diabrotica spectosa (Brazilian corn rootworm (BZR)).

31. improvement be said method comprising the steps of by the method for the drought tolerance of the farm crop of the crop plants generation of insect pest invasion and attack:

A) polynucleotide of claim 1 are introduced described crop plants,

B) cultivate described crop plants, described polynucleotide are expressed, the expression inhibiting insect pest of wherein said polynucleotide is ingested and because the drought tolerance reduction that the insect invasion and attack cause.

32. produce the method for agricultural-food goods, comprise the plant or its part that obtain claim 12, and prepare the agricultural-food goods from described plant or its part.

33. produce the method for food or feed, comprise the plant or its part that obtain claim 12, and prepare food or feed from described plant or its part.

34. the method for claim 33, wherein said food or feed are oil, meal, albumen, starch, fine powder or silage.

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